CN115109725B - Method for enriching homoacetogenic bacteria at normal temperature and application thereof - Google Patents
Method for enriching homoacetogenic bacteria at normal temperature and application thereof Download PDFInfo
- Publication number
- CN115109725B CN115109725B CN202210792160.9A CN202210792160A CN115109725B CN 115109725 B CN115109725 B CN 115109725B CN 202210792160 A CN202210792160 A CN 202210792160A CN 115109725 B CN115109725 B CN 115109725B
- Authority
- CN
- China
- Prior art keywords
- stage
- sludge
- sodium formate
- culture
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 81
- 230000003403 homoacetogenic effect Effects 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 46
- 239000010802 sludge Substances 0.000 claims abstract description 107
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 63
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 60
- 239000004280 Sodium formate Substances 0.000 claims abstract description 56
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 claims abstract description 56
- 235000019254 sodium formate Nutrition 0.000 claims abstract description 56
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 39
- 239000008103 glucose Substances 0.000 claims abstract description 39
- 230000007704 transition Effects 0.000 claims abstract description 33
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 8
- 239000003112 inhibitor Substances 0.000 claims abstract description 7
- 230000007423 decrease Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000007781 pre-processing Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 11
- 238000004064 recycling Methods 0.000 abstract description 11
- 239000010865 sewage Substances 0.000 abstract description 11
- 230000000696 methanogenic effect Effects 0.000 abstract description 6
- 230000000789 acetogenic effect Effects 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 66
- 238000000855 fermentation Methods 0.000 description 37
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 10
- 235000019253 formic acid Nutrition 0.000 description 10
- 239000000758 substrate Substances 0.000 description 9
- 230000004151 fermentation Effects 0.000 description 7
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 235000013619 trace mineral Nutrition 0.000 description 6
- 239000011573 trace mineral Substances 0.000 description 6
- 239000002028 Biomass Substances 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000037361 pathway Effects 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 241000589220 Acetobacter Species 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 238000005265 energy consumption Methods 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
- 230000002572 peristaltic effect Effects 0.000 description 4
- 150000004666 short chain fatty acids Chemical class 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 230000020477 pH reduction Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 235000021391 short chain fatty acids Nutrition 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 229910003208 (NH4)6Mo7O24·4H2O Inorganic materials 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 229910021591 Copper(I) chloride Inorganic materials 0.000 description 2
- 241000589651 Zoogloea Species 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229940005605 valeric acid Drugs 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 1
- 230000002053 acidogenic effect Effects 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- ZFRVFUWCVYLUIH-UHFFFAOYSA-M sodium vanillate Chemical compound [Na+].COC1=CC(C([O-])=O)=CC=C1O ZFRVFUWCVYLUIH-UHFFFAOYSA-M 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/02—Acetobacter
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/145—Clostridium
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Clinical Laboratory Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention relates to a method for enriching homoacetogenic bacteria at normal temperature and application thereof, belonging to the technical field of biological treatment and recycling of sludge. The method for enriching homoacetogenic bacteria at normal temperature comprises an initial stage, a transition stage and an enrichment stage; the initial stage utilizes glucose; glucose and sodium formate are used as carbon sources in the transition stage, and the adding amount of the sodium formate gradually increases along with the increase of the culture days; the enrichment stage utilizes sodium formate as a carbon source to enrich homoacetogenic bacteria in inoculated sludge; the culture temperature of the initial stage, the transition stage and the enrichment stage is 23-25 ℃. The invention has the advantages that no BES methanogenesis inhibitor is required to be additionally added in the process of enriching the acetogenic bacteria to inhibit the methanogenic bacteria in the sludge and external temperature control measures, and the invention can be widely applied to recycling of excess sludge and sewage treatment in reality and has strong applicability.
Description
Technical Field
The invention relates to the technical field of biological treatment and recycling of sludge, in particular to a method for enriching homoacetogenic bacteria at normal temperature and application thereof.
Background
At present, a large amount of surplus sludge is generated in the sewage treatment process, and the surplus sludge is an aggregate formed by zoogloea formed by various microorganisms and organic matters and inorganic matters adsorbed by the zoogloea, so that the organic content is high; the residual sludge is utilized as a resource, and the residual sludge is developed to be used as an organic carbon source in the biological dephosphorization and biological denitrification processes in sewage treatment and a raw material in industrial synthesis, so that the residual sludge becomes a new research hot spot.
And (3) performing anaerobic fermentation on the excess sludge to convert organic matters in the excess sludge into short-chain fatty acids serving as a carbon source for biological nitrogen and phosphorus removal. The anaerobic fermentation process of the sludge can be divided into a series of stages of hydrolysis, acidification, acetic acid production, methane production and the like; acetic acid is an important biosynthesis raw material and is also an important intermediate product in the anaerobic digestion process, so that the generation of methane is inhibited and the acetic acid is enriched in the anaerobic fermentation process, more carbon sources for biological nitrogen and phosphorus removal are generated, and better recycling utilization of residual sludge is realized.
Homoacetogens are a group of bacteria capable of producing CO by the anaerobic acetyl-coa pathway 2 The anaerobic microorganism which is used as a terminal electron acceptor to reduce the terminal electron acceptor to generate acetic acid is enriched, which is favorable for accumulation of short-chain fatty acid mainly comprising acetic acid in the anaerobic fermentation process, and has great significance for recycling of excess sludge.
At present, glucose, sodium formate, sodium vanillate and the like are mostly used as carbon sources for enrichment of homoacetogenic bacteria in the conventional method; meanwhile, BES methanogenesis inhibitor is needed to be added to inhibit methanogenesis in sludge in the process of enriching acetogenesis bacteria, and an external temperature control measure is needed to control the enrichment temperature to about 35 ℃, so that the enrichment cost is high, the energy consumption is high, the enrichment condition requirement is high, and the method is difficult to be applied to recycling of residual sludge and sewage treatment in reality on a large scale and has poor applicability.
Disclosure of Invention
The invention aims to overcome the defects that BES methanogenesis inhibitor is needed to be added to inhibit methanogenesis in sludge in the existing process of enriching acetogenesis, the enrichment temperature is controlled to 35 ℃ by external temperature control measures, the cost is high, the energy consumption is high, the enrichment condition requirement is high, and the method cannot be applied to recycling of residual sludge and sewage treatment in reality on a large scale, and the applicability is poor.
In order to solve the above-mentioned purpose, the invention provides the following technical scheme:
in a first aspect, the present application provides a method for enriching homoacetogenic bacteria at normal temperature, which adopts the following technical scheme:
a method for enriching homoacetogenic bacteria at normal temperature comprises three culture stages of an initial stage, a transition stage and an enrichment stage;
in the initial stage, glucose is used as a carbon source to carry out enlarged culture on seed sludge;
the transition stage uses glucose and sodium formate as carbon sources to selectively culture the inoculated sludge subjected to the expansion culture, and the adding amount of the sodium formate is gradually increased along with the increase of culture days;
the enrichment stage utilizes sodium formate as a carbon source, and further enriches and cultures the inoculated sludge subjected to the selective culture to obtain sludge enriched with homoacetogenic bacteria;
the culture temperature of the initial stage, the transition stage and the enrichment stage is 23-25 ℃.
By adopting the technical scheme, glucose is used as a carbon source for carrying out enlarged culture on all strains in the inoculated sludge in the initial stage, so that the number of homoacetogenic bacteria in the inoculated sludge is increased, and the subsequent selective and enrichment culture is facilitated;
glucose is still used as a part of carbon source in the transition stage, sodium formate is added as the carbon source, and the added amount of the sodium formate is increased, so that microorganisms in inoculated sludge are gradually adapted to the environment with the sodium formate as the carbon source, homoacetogenic bacteria can be selectively cultured, the competitiveness of the homoacetogenic bacteria in inoculated sludge is increased, and the subsequent enrichment of homoacetogenic bacteria by using only the sodium formate as the carbon source is facilitated;
sodium formate is used as a carbon source in the enrichment stage, and homoacetogenic bacteria in the inoculated sludge are enriched;
at present, homoacetogens are generally enriched under the conditions of inhibiting methanogenesis and medium temperature, the environmental adaptability of microorganisms to the carbon source of sodium formate is improved by gradually reducing the dosage of glucose while sodium formate is added, and then the homoacetogens are enriched by using only sodium formate as the carbon source; in the technical scheme, the dosage of sodium formate is gradually increased, and homoacetogenic bacteria can simultaneously take glucose and sodium formate as carbon sources, so that the degradation of glucose by homoacetogenic bacteria is beneficial to the degradation of sodium formate; the carbon source is put in at the temperature of 23-25 ℃ by using the method, so that homoacetogenic bacteria have larger competitiveness than methanogenic bacteria and other strains, and therefore, better enrichment effect of the homoacetogenic bacteria can be realized without adding additional methanogenic inhibitors;
in the technical scheme, the enrichment temperature is 23-25 ℃ and is close to the room temperature, so that no extra temperature control measures are needed, the energy consumption is reduced, no extra inhibitor is needed to be added to inhibit methanogens, the enrichment cost is low, the method can be widely applied to recycling of residual sludge and sewage treatment in reality, the applicability is high, and the method has good popularization and application prospects.
Preferably, the inoculated sludge is prepared by filtering and preprocessing the residual sludge by a 50-mesh sieve and heating and boiling the residual sludge.
By adopting the technical scheme, the excess sludge is filtered and boiled to prepare the inoculated sludge, so that the dissolution of organic matters in the sludge is quickened, the hydrolysis in the anaerobic fermentation process can be shortened, and the subsequent enrichment of homoacetogenic bacteria is facilitated.
Preferably, in the initial stage, the glucose administration amount gradually increases with the number of days of culture.
Preferably, in the transition stage, the initial administration amount of the sodium formate is 0.8-1.2g/L, and the administration amount of the sodium formate is increased by 1.0-2.0g/L every 15-20 days.
Preferably, in the transition stage, the initial administration amount of the sodium formate is 1.0g/L, and the administration amount of the sodium formate is increased by 1.0-1.3g/L every 18-20 days.
Preferably, in the transition stage, the dosage of glucose gradually decreases with the increase of the culture days, and the dosage of glucose is 3.0-4.0g/L.
By adopting the technical scheme, glucose gradually decreases along with the increase of culture days, but the dosage is still kept at 3.0-4.0g/L, the initial dosage of sodium formate is 0.8-1.2g/L, glucose and sodium formate are used as carbon sources in a transition stage, the dosage of sodium formate is increased according to the increasing amplitude of 1.0-2.0g/L every 15-20 days, the dosage of sodium formate in the transition stage is in gradient growth, so that homoacetogenic bacteria in inoculated sludge gradually adapt to the environment with sodium formate as a carbon source, the competitiveness of homoacetogenic bacteria in inoculated sludge strains is strong, the proportion of homoacetogenic bacteria in inoculated sludge is gradually increased, and the selective culture of homoacetogenic bacteria in inoculated sludge is realized, so that the homoacetogenic bacteria become dominant strains.
Preferably, in the enrichment phase, the amount of sodium formate added gradually increases with the number of days of culture.
By adopting the technical scheme, along with the increase of the culture time, the input amount of carbon sources in the system is correspondingly increased, and the normal life activities of homoacetogenic bacteria in inoculated sludge are ensured.
In a second aspect, the present application provides a method for enriching homoacetogenic bacteria at normal temperature according to any one of the above, wherein the sludge enriched with homoacetogenic bacteria is prepared by adopting the following technical scheme:
the sludge enriched with homoacetogenic bacteria has absolute abundance of 1.24×10 14 Copy number per gram of wet sludge, and the homoacetogenic flora accounts for 38-40%.
By adopting the technical scheme, the sludge prepared by the method is analyzed to obtain: the absolute abundance of the key functional gene fhs in homoacetogenic pathway is 1.24X10 14 Copy number per gram of wet sludge, compared with the enrichment1.09×10 before collection 12 The copy number per gram of wet sludge has good enrichment effect, the duty ratio of homoacetogenic bacteria is 39.32 percent, and the homoacetogenic bacteria are dominant in all bacteria of the sludge.
In a third aspect, the present application provides an anaerobic fermentation apparatus adopting the following technical scheme:
an anaerobic fermentation device comprises the sludge enriched with homoacetogenic bacteria.
By adopting the technical scheme, the inoculation sludge is treated by using a method for enriching homoacetogenic bacteria at normal temperature, so that enrichment of homoacetogenic bacteria in the inoculation sludge is realized, short chain fatty acids such as acetic acid and the like are biologically synthesized by homoacetogenic bacteria in an anaerobic fermentation device and serve as carbon sources for microbial nitrogen and phosphorus removal, and resource utilization of residual sludge is realized; the anaerobic fermentation device containing the inoculated sludge enriched with homoacetogenic bacteria is used for carrying out anaerobic fermentation on biomass, so that the yield of acetic acid is greatly improved, and the method has a great application prospect in the aspects of carbon dioxide recycling and carbon emission reduction.
In a fourth aspect, the present application provides an application of the sludge enriched with homoacetogenic bacteria in sewage treatment or biomass resource utilization.
The beneficial effects are that:
(1) Culturing the treated inoculated sludge in stages, and performing expansion culture on strains in the inoculated sludge by using glucose as a carbon source in an initial stage; then, in the transitional stage, under the condition that the glucose dosage is kept within a certain range, the dosage of sodium formate is gradually increased, so that the strain in the inoculated sludge is gradually adapted to the environment taking sodium formate as a carbon source, and homoacetogenic bacteria are selectively cultured at the same time, and the homoacetogenic bacteria have larger competitiveness than the strain such as methanogenic bacteria in the inoculated sludge under the culture condition, and become dominant strain in the inoculated sludge; finally, sodium formate is used as a carbon source in the enrichment stage, so that further enrichment culture of homomethanogens is realized, acetic acid in anaerobic fermentation is used as a main fermentation product, and the acetic acid can be used as a carbon source for biological nitrogen removal and oxygen removal and also can be used as a raw material for industrial synthesis, and the recycling utilization of residual sludge is realized;
(2) In the transition stage, a certain glucose throwing amount is kept in a certain range, the throwing amount of sodium formate is increased in a stage manner, and the homoacetogenic bacteria have larger competition force than methanogenic bacteria at 23-25 ℃ according to the feeding manner, so that no additional methanogenic inhibitor is needed to be added in the enrichment process, no additional temperature control measures are needed to heat and preserve the enrichment system, the energy consumption is lower, and the practical application prospect is wide.
Drawings
In order to more clearly illustrate the technical solutions of the present invention, the following brief description will be made on the accompanying drawings, which are given by way of illustration only and not limitation of the present invention.
FIG. 1 is a schematic diagram of an anaerobic fermentation apparatus according to embodiment 7 of the present invention;
FIG. 2 is a graph showing the results of the generation of volatile acids in the anaerobic fermentation apparatus according to the experimental example of the present invention;
FIG. 3 (a) is a graph showing the results of carbon flow in the P3 stage and the P10 stage of the anaerobic fermentation apparatus according to the embodiment of the present invention;
FIG. 3 (b) is a graph showing the results of the carbon flow in the P3 stage and the P10 stage of the anaerobic fermentation apparatus according to the embodiment of the present invention.
Detailed Description
Preferred embodiments of the present invention will be described in more detail below. While the preferred embodiments of the present invention are described below, it should be understood that the present invention may be embodied in various forms and should not be limited to the embodiments set forth herein. The specific techniques or conditions are not identified in the examples and are performed according to techniques or conditions described in the literature in this field or according to the product specifications. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention. In the examples below, "%" refers to weight percent, unless explicitly stated otherwise.
The application provides a method for enriching homoacetogenic bacteria at normal temperature, which comprises an initial stage, a transition stage and an enrichment stage;
in the initial stage, glucose is used as a carbon source to carry out enlarged culture on seed sludge;
the transition stage uses glucose and sodium formate as carbon sources to selectively culture the inoculated sludge subjected to the expansion culture, and the adding amount of the sodium formate is gradually increased along with the increase of culture days;
the enrichment stage further enriches the inoculated sludge subjected to the selective culture by using sodium formate as a carbon source to obtain sludge enriched with homoacetogenic bacteria;
the culture temperature of the initial stage, the transition stage and the enrichment stage is 23-25 ℃;
preferably, the culture temperature is 24 ℃.
In the invention, the Mixed Liquor Suspended Solids (MLSS) and the Mixed Liquor Volatile Suspended Solids (MLVSS) of the inoculated sludge are 42.4g/L and 13.9g/L respectively.
In the invention, trace elements and minerals are also added into the enrichment system to provide nutrients necessary for the growth and propagation of microorganisms, including the following substances: trace elements (mg/L): feCl 2 ·H 2 O,2；H 3 BO 3 ，0.05；ZnCl 2 ，0.05；CuCl 2 ·2H 2 O，0.038；MnCl 2 ·4H 2 O，0.05；(NH 4 ) 6 Mo 7 O 24 ·4H 2 O，0.05；AlCl 3 ,0.05；CoCl 2 ·6H 2 O，0.05；NiCl 2 ·6H 2 O，0.092；Na 2 WO 4 ·2H 2 O,0.05; mineral (mg/L): caCl (CaCl) 2 ，0.05；MgCl 2 ·6H 2 O，0.1；NaCl,0.1。
In one embodiment, the inoculated sludge is prepared by filtering the excess sludge with a 50 mesh sieve, and heating to boil.
In the invention, after filtering the surplus sludge by using a 50-mesh sieve to remove impurities, the filtered sludge is boiled for 2 hours at the temperature of 100 ℃. In one embodiment, in the initial stage, the glucose dosage gradually increases with the number of days of culture.
In one embodiment, the initial dosage of sodium formate is 0.8-1.2g/L and the dosage of sodium formate is increased by 1.0-2.0g/L every 15-20 days during the transition phase.
In one embodiment, the initial dosage of sodium formate is 1.0g/L and the dosage of sodium formate is increased by 1.0-1.3g/L every 18-20 days during the transition phase.
In one embodiment, in the transition stage, the dosage of glucose gradually decreases with the increase of the culture days, and the dosage of glucose is 3.0-4.0g/L.
In the invention, in the transition stage, although the dosage of glucose is gradually reduced, the dosage of glucose still keeps within the range of 3.0-4.0g/L, and the dosage of sodium formate is gradually increased so that the strain in the inoculated sludge is suitable for the environment with sodium formate as a carbon source, and homoacetogenic bacteria are selectively cultured.
In one embodiment, in the enrichment phase, the amount of sodium formate dosed increases with the number of days of culture.
In the invention, the number of the strains in the inoculated sludge is increased along with the increase of the culture days, so that the input amount of a carbon source is also required to be increased, and the normal life activities of the strains in the inoculated sludge are ensured.
The application also provides the sludge enriched with homoacetogenic bacteria, which is prepared by the method for enriching homoacetogenic bacteria at normal temperature, wherein the absolute abundance of homoacetogenic bacteria in the sludge enriched with homoacetogenic bacteria is 1.24 multiplied by 10 14 Copy number per gram of wet sludge, and the homoacetogenic flora accounts for 38-40%.
The application also provides an anaerobic fermentation device comprising the sludge enriched with homoacetogenic bacteria.
The application also provides application of the sludge enriched with homoacetogenic bacteria in sewage treatment or biomass resource utilization.
According to the invention, the anaerobic fermentation technology is used for treating biomass such as sewage treatment and organic waste, wherein sludge enriched with homoacetogenic bacteria is added into an anaerobic fermentation system to improve the hydrolysis and acidification efficiency of organic matters in the anaerobic fermentation process, so that more carbon flows to volatile short-chain fatty acids such as formic acid and acetic acid in the anaerobic fermentation, and the biomass can be used as a carbon source for biological nitrogen and phosphorus removal and can also be used as a raw material for industrial production.
Example 1 method for enriching homoacetogenic bacteria at Normal temperature
In the embodiment, after filtering and removing impurities from the residual sludge generated in the sewage treatment process by using a 50-mesh sieve, heating and boiling the filtered sludge at 100 ℃ for 2 hours to obtain inoculated sludge, wherein the Mixed Liquor Suspended Solids (MLSS) and the Mixed Liquor Volatile Suspended Solids (MLVSS) in the inoculated sludge are 42.4g/L and 13.9g/L respectively.
Glucose is used as a carbon source in the initial stage; adding sodium formate under the condition of keeping the adding amount of glucose unchanged in the transition stage, and taking glucose and sodium formate as carbon sources; the enrichment stage separately uses sodium formate as a carbon source; in the whole enrichment process, ammonium chloride and monopotassium phosphate/dipotassium phosphate are used as nitrogen sources and phosphorus sources, and trace elements and minerals are added to provide nutrients necessary for the growth and propagation of microorganisms, wherein the specific components and concentrations are as follows: trace elements (mg/L): feCl 2 ·H 2 O,2；H 3 BO 3 ，0.05；ZnCl 2 ，0.05；CuCl 2 ·2H 2 O，0.038；MnCl 2 ·4H 2 O，0.05；(NH 4 ) 6 Mo 7 O 24 ·4H 2 O，0.05；AlCl 3 ,0.05；CoCl 2 ·6H 2 O，0.05；NiCl 2 ·6H 2 O，0.092；Na 2 WO 4 ·2H 2 O,0.05; mineral (mg/L): caCl (CaCl) 2 ，0.05；MgCl 2 ·6H 2 O,0.1; naCl,0.1; and add a proper amount of NaHCO 3 So that the pH of the enrichment system is maintained between 6.8 and 7.2.
The substrates added in the initial stage, transition stage and enrichment stage are shown in Table 1:
TABLE 1 substrate addition Table of example 1
EXAMPLE 2 method for enriching homoacetogenic bacteria at Normal temperature
This example differs from example 1 in that the incubation temperature in the initial, transition and enrichment stages was 23 ℃.
Example 3 method for enriching homoacetogenic bacteria at Normal temperature
This example differs from example 1 in that the incubation temperature in the initial, transition and enrichment stages was 25 ℃.
EXAMPLE 4 method for Normal temperature enrichment of homoacetogenic bacteria
This example differs from example 1 in the amount of substrate added during the transition phase, as shown in Table 2:
TABLE 2 substrate addition Table of example 4
EXAMPLE 5 method for enriching homoacetogenic bacteria at Normal temperature
This example differs from example 1 in the amount of substrate added during the transition phase, as shown in Table 3:
TABLE 3 substrate addition Table of example 5
EXAMPLE 6 sludge enriched in homoacetogens
The sludge enriched with homoacetogenic bacteria provided by the embodiment is prepared by the method provided by the embodiment 1-5; wherein the absolute abundance of the key functional gene fhs in the homoacetogenic pathway in the sludge is 1.24X10 14 Copy number per gram of wet sludge, ratio before enrichment 1.09×10 12 The copy number per gram of wet sludge is obviously improved. The homoacetogenic flora accounts for 39.32 percent and is the dominant flora in the sludge.
Example 7 anaerobic fermentation apparatus
The anaerobic fermentation device provided by the embodiment has a structure shown in figure 1, and comprises a sample injection barrel, a reactor and a gas collection bag, wherein a peristaltic pump is further arranged between the sample injection barrel and the reactor, and the sample injection barrel is communicated with the peristaltic pump and the peristaltic pump is communicated with the reactor through pipelines in sequence; the reactor is filled with the sludge enriched with homoacetogenic bacteria provided in the embodiment 6, and is also internally provided with stirring paddles, the joint of the stirring paddles and the reactor is provided with a silica gel pad for preventing air leakage, and the air collecting bag is communicated with the upper part of the reactor through a pipeline.
Before the anaerobic fermentation device is formally operated, the method for enriching homoacetogenic bacteria at normal temperature provided in the examples 1-5 is used for treating inoculated sludge to obtain sludge enriched with homoacetogenic bacteria, or the sludge enriched with homoacetogenic bacteria provided in the example 6 is directly added into a reactor; the anaerobic fermentation substrates such as wastewater are placed in a sample injection barrel and enter the reactor through a peristaltic pump at a constant flow rate; when anaerobic fermentation is carried out in the reactor, the stirring paddle is used for mechanically stirring the wastewater and the sludge, so that more carbon flows to formic acid, acetic acid and other low-carbon volatile fatty acids in the anaerobic fermentation carried out in the anaerobic fermentation device, and the anaerobic fermentation device is used as an organic carbon source in the biological dephosphorization and biological denitrification processes in sewage treatment and as a raw material in industrial synthesis, and the recycling utilization of the residual sludge is better realized.
Experimental example
Performing normal temperature enrichment of homoacetogenic bacteria by using the anaerobic fermentation device provided in the embodiment 7 and the method for enriching homoacetogenic bacteria provided in the embodiment 1; wherein, the anaerobic fermentation device is fed water by artificial simulation, the pH of the fed water is regulated and controlled to 7, and the hydraulic retention time is 8h; after the enrichment culture of the homoacetogenic bacteria of the sludge is finished, continuously carrying out anaerobic fermentation by taking glucose as a carbon source, wherein the dosage of the glucose is 4.0g/L; and the oxidation-reduction potential (ORP), gas volume, gas composition, and effluent Volatile Fatty Acid (VFAs) composition and content were detected in the above process, and the results are described below.
1. Volatile acid generation
FIG. 2 is a graph showing the results of the generation of volatile acids in an anaerobic fermentation apparatus, from which it can be seen that the VFAs concentration in the apparatus gradually increases during the initial stage (P1-P3) and that the OLR is higher; in the transition stage (P4-P6), the concentration of the OLR and the total VFAs are close to those in the P3 stage, and along with the increase of the sodium formate input amount and the decrease of the glucose input amount, the concentration of acetic acid in the VFAs is increased, the yield of acetic acid is obviously improved, the selective culture of homoacetogenic bacteria is realized by adding the sodium formate, and the generation of acetic acid is promoted; in the enrichment stage (P7-P9), the yield of the acetic acid is better, and along with the increase of the sodium formate dosage, the yield of the acetic acid is obviously improved, and reaches about 1000mg HAc/L in the P8 stage, and reaches about 1500mg HAc/L in the P9 stage.
P10 is a stage of continuously carrying out anaerobic fermentation by taking glucose as a carbon source after enrichment culture of the sludge homoacetogenic bacteria is finished; at P10, acetic acid and n-butyric acid are the primary VFAs produced in the device, using only glucose as a carbon source; and, comparing the P3 stage with the P10 stage, the yield of acetic acid in the P10 stage reaches more than 700mg HAc/L, which is improved by about 21% compared with that of P3, and the yield of formic acid in the P10 stage is about 60-70mg HAc/L, the sludge treated by the method provided by the embodiment 1 is applied to anaerobic fermentation, so that more carbon flows to formic acid and acetic acid in the anaerobic fermentation process, the pressure of the volatile acid interaction degradation of more than two carbon is reduced, and the risk of systematic acidification caused by the accumulation of VFAs of more than two carbon is also reduced.
2. In-device carbon flow analysis
ResultsAs shown in fig. 3 (a), 3 (b), 0.02% of the carbon flowed to the biogas and 31.39% to the VFAs in the P3 stage, wherein the carbon flowed to the propionic acid, butyric acid and valeric acid accounted for 19.3% and the carbon flowed to the formic acid and acetic acid accounted for only 0.22% and 11.72%, indicating weak homoacetogenic effect in the initial stage. After enrichment, the carbon flowing to the biogas in the P10 stage is similar to that in the P1-P9 stages, and only accounts for 0.02 percent, but only 0.02 percent of CO is generated in the P10 2 The carbon flowing to the VFAs is obviously increased to 34.46 percent of the total VFAs, wherein the carbon six flowing to formic acid and acetic acid respectively accounts for 2.26 percent and 16.82 percent, and the carbon six flowing to propionic acid, butyric acid and valeric acid is reduced to 15.39 percent, which shows that the anaerobic fermentation is carried out by using the inoculated sludge enriched by homoacetogenic bacteria, so that more carbon flows to formic acid, acetic acid and other low-carbon volatile fatty acids.
3. Sludge colony analysis
With formic acid or H 2 /CO 2 The bacteria are enriched for the substrate, acetobacter homoacetogens and glucose substrate, clostridium_sensu_stricto_1 homoacetogens. During the P3 phase prior to enrichment, the relative abundances of Acetobacter and Clostridium_sendu_stricto_1 were 0.22% and 30.15%, respectively, and the absolute abundance of the critical functional gene fhs in the homoacetogenic pathway was 1.09×10 12 Copy number per gram of wet sludge. In the stage P7-P9, along with the continuous increase of the concentration of formic acid in the water, the relative abundance of Acetobacter is gradually increased to 37.52%, which shows that the addition of formic acid has obvious enrichment effect on homoacetogenic bacteria in normal temperature environment. At the P10 stage after enrichment was completed, the relative abundance of Acetobacter and Clostridium_sendu_stricto_1 was 13.38% and 25.93%, respectively. The absolute abundance of the key functional gene fhs in homoacetogenic pathway is 1.24X10 14 The copy number per gram of wet sludge shows that the homoacetogenic flora gradually becomes dominant flora in an acidogenic system after enrichment.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner without contradiction. The various possible combinations of the invention are not described in detail in order to avoid unnecessary repetition.
Moreover, any combination of the various embodiments of the invention can be made without departing from the spirit of the invention, which should also be considered as disclosed herein.
Claims (4)
1. A method for enriching homoacetogenic bacteria at normal temperature is characterized by comprising the following steps: comprises an initial stage, a transition stage and an enrichment stage;
in the initial stage, glucose is used as a carbon source to carry out enlarged culture on seed sludge;
the transition stage uses glucose and sodium formate as carbon sources to selectively culture the inoculated sludge subjected to the expansion culture, and the adding amount of the sodium formate is gradually increased along with the increase of culture days;
the enrichment stage further enriches the inoculated sludge subjected to the selective culture by using sodium formate as a carbon source to obtain sludge enriched with homoacetogenic bacteria;
the culture temperature of the initial stage, the transition stage and the enrichment stage is 23-25 ℃, and no additional inhibitor is needed to inhibit methanogens;
in the transition stage, the initial adding amount of the sodium formate is 0.8-1.2g/L, and the adding amount of the sodium formate is increased by 1.0-2.0g/L every 15-20 days;
in the transition stage, the dosage of glucose gradually decreases along with the increase of the culture days, and the dosage of glucose is 3.0-4.0g/L;
in the enrichment stage, the adding amount of the sodium formate is gradually increased along with the increase of the culture days.
2. The method for enriching homoacetogenic bacteria at normal temperature according to claim 1, wherein the method comprises the following steps: the inoculation sludge is prepared by filtering and preprocessing the residual sludge by a 50-mesh sieve, and heating and boiling the residual sludge.
3. The method for enriching homoacetogenic bacteria at normal temperature according to claim 1, wherein the method comprises the following steps: in the initial stage, the glucose administration amount gradually increases with the increase of the culture days.
4. The method for enriching homoacetogenic bacteria at normal temperature according to claim 1, wherein the initial dosage of sodium formate is 1.0g/L and the dosage of sodium formate is increased by 1.0-1.3g/L every 18-20 days in the transition stage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210792160.9A CN115109725B (en) | 2022-07-07 | 2022-07-07 | Method for enriching homoacetogenic bacteria at normal temperature and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210792160.9A CN115109725B (en) | 2022-07-07 | 2022-07-07 | Method for enriching homoacetogenic bacteria at normal temperature and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115109725A CN115109725A (en) | 2022-09-27 |
| CN115109725B true CN115109725B (en) | 2024-02-27 |
Family
ID=83332216
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210792160.9A Active CN115109725B (en) | 2022-07-07 | 2022-07-07 | Method for enriching homoacetogenic bacteria at normal temperature and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115109725B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433278A (en) * | 2011-12-13 | 2012-05-02 | 江南大学 | Enrichment culture method of homoacetogenic bacteria |
| CN104263764A (en) * | 2014-09-15 | 2015-01-07 | 常州大学 | Process for high-efficiency anaerobic production of acetic acid with homoacetogenic bacteria-rich seed sludge |
-
2022
- 2022-07-07 CN CN202210792160.9A patent/CN115109725B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433278A (en) * | 2011-12-13 | 2012-05-02 | 江南大学 | Enrichment culture method of homoacetogenic bacteria |
| CN104263764A (en) * | 2014-09-15 | 2015-01-07 | 常州大学 | Process for high-efficiency anaerobic production of acetic acid with homoacetogenic bacteria-rich seed sludge |
Non-Patent Citations (2)
| Title |
|---|
| pH值和产甲烷抑制剂对两相耦合系统污泥发酵定向产乙酸的影响;扶郡;刘和;王新华;李秀芬;陈坚;;工业微生物(第06期);全文 * |
| 污泥厌氧消化中同型产乙酸菌富集研究;王晋 等;工业微生物;第43卷(第1期);摘要，第1-2页 第1.1节，第3页 右栏 第1段，第3页 右栏 第1段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115109725A (en) | 2022-09-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20210115477A1 (en) | System for the production of methane from co2 | |
| Mamimin et al. | Two-stage thermophilic fermentation and mesophilic methanogen process for biohythane production from palm oil mill effluent | |
| Mohan et al. | Anaerobic biohydrogen production from dairy wastewater treatment in sequencing batch reactor (AnSBR): effect of organic loading rate | |
| EP2032709B1 (en) | System for the production of methane from co2 | |
| CN108314184B (en) | Method for promoting start of anaerobic reactor | |
| Chen et al. | Start‐up of anaerobic hydrogen producing reactors seeded with sewage sludge | |
| CN102557349B (en) | Process and device of low energy consumption sewage treatment based on carbon source recovery | |
| Lin et al. | Evaluation of the synergistic effects of biochar and biogas residue on CO2 and CH4 emission, functional genes, and enzyme activity during straw composting | |
| Li et al. | Chemically enhanced primary sedimentation and acidogenesis of organics in sludge for enhanced nitrogen removal in wastewater treatment | |
| Xie et al. | Biogas conditioning and digestate recycling by microalgae: Acclimation of Chlorella vulgaris to H2S-containing biogas and high NH4-N digestate and effect of biogas: Digestate ratio | |
| CN108383239B (en) | Integrated biological treatment process for shortcut nitrification anaerobic ammonia oxidation and phosphorus removal under intermittent aeration mode | |
| Guo et al. | Comparison of primary and secondary sludge carbon sources derived from hydrolysis or acidogenesis for nitrate reduction and denitrification kinetics: organics utilization and microbial community shift | |
| CN111484138A (en) | Control method for oxidation coupling denitrification process for synchronously producing methane and aerobic methane | |
| CN111484133A (en) | Reaction system and treatment method for enhancing micro-aerobic methane-producing granular sludge | |
| CN109401947B (en) | Sludge and kitchen waste co-digestion system and operation method thereof | |
| KR101990059B1 (en) | Apparatus and method of producing high purity methane gas using gas recycle | |
| CN107162214B (en) | Sewage treatment method for nitrogen and phosphorus removal by coupling composite microorganism with micron zero-valent iron | |
| KR100853287B1 (en) | High rate methanc production system using anaerobic archaea | |
| Yan et al. | Attaining superior nitrogen removal from integrated mature landfill leachate and kitchen waste digestion liquid via a two-stage partial nitrification/anammox (PN/A) process | |
| CN115109725B (en) | Method for enriching homoacetogenic bacteria at normal temperature and application thereof | |
| Wang et al. | Effect of the Ca2+ concentration on anaerobic digestion and microbial communities of granular sludge | |
| CN205024060U (en) | Sewage treatment plant excess sludge high temperature hydrolytic acidification device is reinforceed to aeration | |
| CN108249725B (en) | Method for producing methane by pre-fermenting and enhancing anaerobic digestion of excess sludge | |
| CN219860841U (en) | Device for using sludge from garbage leachate treatment process as denitrification additional carbon source | |
| CN107162314B (en) | Method for treating excess sludge and recycling resources and method for improving VFAs yield |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |