CN115107131A - Bamboo mould-proof treatment method coupling white rot fungus pretreatment and bactericide impregnation - Google Patents
Bamboo mould-proof treatment method coupling white rot fungus pretreatment and bactericide impregnation Download PDFInfo
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- CN115107131A CN115107131A CN202210851490.0A CN202210851490A CN115107131A CN 115107131 A CN115107131 A CN 115107131A CN 202210851490 A CN202210851490 A CN 202210851490A CN 115107131 A CN115107131 A CN 115107131A
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K9/00—Chemical or physical treatment of reed, straw, or similar material
- B27K9/002—Cane, bamboo
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K3/00—Impregnating wood, e.g. impregnation pretreatment, for example puncturing; Wood impregnation aids not directly involved in the impregnation process
- B27K3/002—Impregnating wood, e.g. impregnation pretreatment, for example puncturing; Wood impregnation aids not directly involved in the impregnation process employing compositions comprising microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K3/00—Impregnating wood, e.g. impregnation pretreatment, for example puncturing; Wood impregnation aids not directly involved in the impregnation process
- B27K3/02—Processes; Apparatus
- B27K3/0228—Spraying apparatus, e.g. tunnels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K3/00—Impregnating wood, e.g. impregnation pretreatment, for example puncturing; Wood impregnation aids not directly involved in the impregnation process
- B27K3/02—Processes; Apparatus
- B27K3/08—Impregnating by pressure, e.g. vacuum impregnation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K3/00—Impregnating wood, e.g. impregnation pretreatment, for example puncturing; Wood impregnation aids not directly involved in the impregnation process
- B27K3/34—Organic impregnating agents
- B27K3/343—Heterocyclic compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K3/00—Impregnating wood, e.g. impregnation pretreatment, for example puncturing; Wood impregnation aids not directly involved in the impregnation process
- B27K3/34—Organic impregnating agents
- B27K3/36—Aliphatic compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K3/00—Impregnating wood, e.g. impregnation pretreatment, for example puncturing; Wood impregnation aids not directly involved in the impregnation process
- B27K3/34—Organic impregnating agents
- B27K3/50—Mixtures of different organic impregnating agents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K5/00—Treating of wood not provided for in groups B27K1/00, B27K3/00
- B27K5/0085—Thermal treatments, i.e. involving chemical modification of wood at temperatures well over 100°C
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K5/00—Treating of wood not provided for in groups B27K1/00, B27K3/00
- B27K5/04—Combined bleaching or impregnating and drying of wood
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K2240/00—Purpose of the treatment
- B27K2240/20—Removing fungi, molds or insects
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Forests & Forestry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Chemical And Physical Treatments For Wood And The Like (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a bamboo mildew-proof treatment method coupling white rot fungus pretreatment and bactericide impregnation, which comprises the following steps: s1, preparing a white rot fungus suspension; s2, pretreating bamboo wood by adopting white rot fungus suspension; s3, performing water bath treatment and drying treatment on the pretreated bamboo wood; s4, preparing an organic bactericide; s5, pressurizing and dipping the organic bactericide; and S6, post-processing the bamboo.
Description
Technical Field
The invention relates to the technical field of bamboo biochemical synergistic mildew-proof treatment protection, in particular to a bamboo mildew-proof treatment method coupling white rot fungus pretreatment and bactericide impregnation.
Background
The bamboo resources in China are very rich, the area of the bamboo forest and the yield of the bamboo are at the top of the world, the variety of products exceeds 10000, and the industrial output value is about 2000 hundred million yuan. The bamboo has the characteristics of reproducibility, high strength, high yield, easy processing and the like, so that the replacement of wood by bamboo becomes one of effective ways for making up the shortage of high-quality wood resources. Bamboo contains more nutrients than wood, about 2-5% starch, 1.5-6% protein and 2% glucose, wherein starch is the main food source for fungi, so that bamboo and bamboo products are more easily damaged by fungi and pests. The mildew of the bamboo wood not only affects the use value and the economic value of the bamboo wood and bamboo products, but also has certain harm to human bodies. Therefore, developing the study of the bamboo wood mildew-proof treatment is one of the important measures for realizing the efficient utilization of the bamboo wood.
In recent years, more and more scholars research on bamboo modification, and common methods for bamboo mildew-proof treatment include high-temperature carbonization, chemical modification, bactericide impregnation, biological pretreatment and the like, wherein the method is still bactericide impregnation treatment after being most widely applied. Patent CN108908594B discloses a treatment method for prolonging the effective time of mildew-proof bamboo wood, which utilizes isothiazolinone bactericides to modify the bamboo wood. ZL201610631765.4 discloses a triazole wood and bamboo wood anticorrosion and antitarnish composition and a preparation method thereof to prepare a triazole and isothiazolinone bactericide composite preparation which has mildew-proof, anticorrosion and antitarnish performances. Although the method achieves certain effect, the modified bamboo still has the technical defects of low prevention and treatment effect, short time effect and the like when used outdoors, and long-acting mildew prevention of the bamboo for outdoor use cannot be realized all the time.
Therefore, aiming at the technical problems in the prior art, it is necessary to design a novel bamboo wood coupling mildew-proof biochemical treatment method.
Disclosure of Invention
In order to overcome the defects of low prevention and treatment effect and short prevention and treatment time efficiency of a bamboo mildew prevention technology in the prior art, the invention provides a bamboo mildew prevention treatment method by coupling white rot fungus pretreatment and bactericide impregnation. Pretreating bamboo wood by white rot fungi, and then performing immersion treatment by using a bactericide; the specific method comprises the following steps: inoculating white rot fungi on the surface of bamboo wood, placing the bamboo wood in a constant-temperature constant-humidity incubator with the temperature of 28 +/-0.2 ℃ and the relative humidity of 85% to culture for 10, 20 and 30 days and the like, and then adopting an organic bactericide to carry out pressure impregnation treatment on the fungus pretreatment modified bamboo wood, wherein the pressure is 0.5 MPa-1.5 MPa, and the time is 40-60 min. After the modified bamboo wood is subjected to 28-day mildew experiments, hypha and mildew points do not exist on the surface, the using amount of the bactericide is effectively reduced, the leaching resistance and the antibiotic degradability of the bactericide are improved, and green and long-acting mildew prevention of the bamboo wood is realized。The modification condition is mild, the cost is low, the method is green and environment-friendly, the application prospect is good, and a new method is provided for the mildew-proof treatment of the bamboo wood.
The invention aims to provide a bamboo wood mildew-proof treatment method for coupling white rot fungus pretreatment and bactericide impregnation, which comprises the following steps:
s1, preparing a white rot fungus suspension;
s2, pretreating the bamboo wood by adopting the white rot fungus suspension;
s3, performing water bath treatment and drying treatment on the pretreated bamboo wood;
s4, preparing an organic bactericide;
s5, carrying out pressure impregnation on the organic bactericide;
and S6, performing post-treatment on the bamboo wood.
In a preferred embodiment, the white rot fungi are Coriolus versicolor (trameteversicolor), Phanerochaete chrysosporium (Phanerochaethrichrysosporium), Pleurotus ostreatus (Pleurotusostreatus) or Phellinus baumii (Phellinus baumii).
As a preferred embodiment, the S1 includes:
s11, transferring the white rot fungi from the slant culture medium to a plate filled with a potato agar culture medium;
s12, activating at 28 + -0.2 deg.C and 85% relative humidity;
s13, putting the fungus cake with the diameter of 1.5cm into a beaker, adding 200mL of sterile water, and uniformly stirring by using a stirrer to prepare white rot fungus suspension for later use.
As a preferred embodiment, the S2 includes:
s21, uniformly inoculating the white rot fungus suspension to bamboo materials in a soaking or spraying mode, wherein the soaking time in the white rot fungus suspension is 2-20 min under the soaking condition; under the condition of adopting the spraying, the spraying amount of the white rot fungus suspension is 0.5 kg-2 kg/m 2 ；
S22, transferring the inoculated bamboo wood into a constant temperature and humidity incubator, and culturing at the temperature of 28 +/-0.2 ℃ and the relative humidity of 85% for 5-50 days.
In a preferred embodiment, the soaking time is 2-10 min, or the spraying amount is 0.5-1.5 kg/m 2 The culture time is 10-30 days.
As a preferred embodiment, the S3 includes:
s31, scraping hypha on the surface of the pretreated bamboo wood;
and S32, placing the mixture into boiling water for hot bath for 0.5-5 hours, taking out the mixture, and drying the mixture at 63 ℃ to constant weight.
In a preferred embodiment, the organic bactericide is an aqueous composite organic bactericide with a total active ingredient of 0.1% to 0.5%, wherein the total active ingredient represents the total concentration of the organic bactericide.
In a preferred embodiment, the organic bactericide comprises 1 to 30 parts of strobilurin, 1 to 50 parts of isothiazolinone and 1 to 40 parts of quaternary ammonium salt.
As a preferred embodiment, the S5 includes: and (3) soaking the organic bactericide into the pretreated bamboo wood, and keeping the vacuum pressure at 0.5-1.5 MPa for 40-60 min.
As a preferred embodiment, the S6 includes: and taking out the bamboo wood subjected to the dipping modification in the S5, transferring the bamboo wood to a drying kiln for post-fixing treatment, wherein the temperature of the post-fixing treatment is 40-80 ℃.
The bamboo mildew-proof treatment method coupling the white rot fungus pretreatment and the bactericide impregnation has the following beneficial effects, and is mainly characterized in that:
(1) the control efficiency is high: the bamboo pretreated by the white rot fungi is dipped in the low-concentration bactericide, so that the surface of the bamboo is not mildewed.
(2) The prevention and treatment time is long: the anti-loss performance and the anti-biological degradability are improved, and the service life of the bamboo and bamboo products in outdoor high-humidity environment is prolonged.
(3) Green and environment-friendly: the method has the advantages of mild reaction conditions, high efficiency, low energy consumption, no pollutant generation and good environmental friendliness.
The above and other objects, advantages and features of the present invention will become more apparent to those skilled in the art from the following detailed description of specific embodiments thereof, taken in conjunction with the accompanying drawings.
Drawings
Some specific embodiments of the invention will be described in detail hereinafter, by way of illustration and not limitation, with reference to the accompanying drawings. The same reference numbers in the drawings identify the same or similar elements or components. Those skilled in the art will appreciate that the drawings are not necessarily drawn to scale. The objects and features of the present invention will become more apparent in view of the following description taken in conjunction with the accompanying drawings, in which:
fig. 1(a) is a schematic diagram illustrating the determination of active ingredients of a bactericide in bamboo in an experiment of influence of pretreatment of white rot fungi on the degradation resistance of the bactericide in bamboo according to a preferred embodiment of the invention;
fig. 1(b) is a schematic diagram illustrating the determination of the active ingredients of the bactericide in the bamboo in the experiment of the influence of the pretreatment of white rot fungi on the leaching resistance of the bactericide in the bamboo according to the preferred embodiment of the invention;
FIG. 2(a) is a schematic view of starch content of white rot fungi pretreated bamboo according to an embodiment of the present invention;
FIG. 2(b) is a schematic diagram illustrating a change in liquid absorption rate of white rot fungi pretreated bamboo according to an embodiment of the present invention;
fig. 3 is a bacteriostatic activity diagram of a white rot fungus metabolite provided according to an embodiment of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "vertical", "upper", "lower", "horizontal", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
The method for bamboo wood mildew-proof treatment by coupling white rot fungus pretreatment and bactericide impregnation comprises the following steps:
s1, preparing a white rot fungus suspension;
s2, pretreating the bamboo wood by adopting the white rot fungus suspension;
s3, performing water bath treatment and drying treatment on the pretreated bamboo wood;
s4, preparing an organic bactericide;
s5, carrying out pressure impregnation on the organic bactericide;
and S6, performing post-treatment on the bamboo wood.
In a preferred embodiment, the white rot fungi are Coriolus versicolor (trameteversicolor), Phanerochaete chrysosporium (Phanerochaethrichrysosporium), Pleurotus ostreatus (Pleurotusostreatus) or Phellinus baumii (Phellinus baumii), all of which are available from the institute of microbiology in the Central academy of sciences.
In this example, it was found that phellinus baumii (phellinus baumii) significantly deteriorates mechanical properties of bamboo materials at the early stage of decay, and thus, the strain of white rot fungi is selected from coriolus versicolor (Trametes versicolor), phanerochaetes chrysosporium (phanerochaetes chrysosporium) or pleurotus ostreatus (Pleurotusostreatus) in many cases.
As a preferred embodiment, the S1 includes:
s11, transferring the white rot fungi from the slant culture medium to a plate filled with a potato agar culture medium (PDA, purchased from Obotaxacum Biotechnology, Inc., Beijing);
s12, activating at 28 + -0.2 deg.C and 85% relative humidity;
s13, putting the fungus cake with the diameter of 1.5cm into a beaker, adding 200mL of sterile water, and uniformly stirring by using a stirrer to prepare white rot fungus suspension for later use.
Coriolus versicolor (trameteversicolor), Phanerochaete chrysosporium (Phanerochaete chrysosporium), Pleurotus ostreatus (Pleurotusostreatus) or Phellinus baumii (Phellinus baumii) can be prepared by the same method.
As a preferred embodiment, the S2 includes:
s21, suspending the white rot fungi by adopting a soaking or spraying modeUniformly inoculating the solution to bamboo (50mm multiplied by 20mm multiplied by 5mm), wherein the soaking time in the white rot fungus suspension is 2-20 min under the condition of soaking; under the condition of adopting the spraying, the spraying amount of the white rot fungus suspension is 0.5 kg-2 kg/m 2 ；
S22, transferring the inoculated bamboo wood into a constant temperature and humidity incubator, and culturing at the temperature of 28 +/-0.2 ℃ and the relative humidity of 85% for 5-50 days.
In a preferred embodiment, the soaking time is 2-10 min, or the spraying amount is 0.5-1.5 kg/m 2 And the culture time is 10-30 days.
As a preferred embodiment, the S3 includes:
s31, scraping hyphae on the surface of the pretreated bamboo wood by using an art designing knife;
and S32, placing the mixture into boiling water for hot bath for 0.5-5 hours, taking out the mixture, and drying the mixture at 63 ℃ to constant weight.
In a preferred embodiment, the organic bactericide is an aqueous composite organic bactericide with a total active ingredient of 0.1% to 0.5%, wherein the total active ingredient represents the total concentration of the organic bactericide.
In a preferred embodiment, the organic bactericide comprises 1 to 30 parts of strobilurin, 1 to 50 parts of isothiazolinone and 1 to 40 parts of quaternary ammonium salt.
As a preferred embodiment, the S5 includes: and (3) impregnating the organic bactericide into the pretreated bamboo wood, and keeping the vacuum pressure at 0.5-1.5 MPa for 40-60 min.
As a preferred embodiment, the S6 includes: and taking out the bamboo wood subjected to the dipping modification in the S5, transferring the bamboo wood to a drying kiln for post-fixing treatment, wherein the temperature of the post-fixing treatment is 40-80 ℃.
Example 1
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 10 days, taking out, and scraping surface hypha. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which consists of 25 parts of methoxy acrylate, 45 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.5MPa, and the negative pressure is kept for 60 min.
Example 2
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 20 days, taking out, and scraping surface mycelium. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which is composed of 25 parts of methoxy acrylate, 50 parts of isothiazolinone and 25 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.5MPa, and the negative pressure is kept for 60 min.
Example 3
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 30 days, taking out, and scraping surface mycelium. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which consists of 30 parts of methoxy acrylic acid esters, 30 parts of isothiazolinone and 40 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Example 4
Sterilizing bamboo, soaking in white rot fungus (Phanerochaete chrysosporium) suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 30 days, taking out, and scraping surface hypha. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which consists of 30 parts of methoxy acrylic acid esters, 50 parts of isothiazolinone and 20 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Example 5
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and relative humidity of 80% for 30 days, taking out, and scraping surface hypha. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which consists of 30 parts of methoxy acrylic acid esters, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Example 6
Sterilizing bamboo, soaking in white rot fungus (Phellinus baumii) suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 30 days, taking out, and scraping surface hypha. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which consists of 30 parts of methoxy acrylic acid esters, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Example 7
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 30 days, taking out, and scraping surface mycelium. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.15% water-based composite organic bactericide, and the bactericide consists of 30 parts of methoxy acrylate, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Example 8
Sterilizing bamboo, soaking in white rot fungus (Phanerochaete chrysosporium) suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 30 days, taking out, and scraping surface hypha. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.15% water-based composite organic bactericide which consists of 30 parts of methoxy acrylic esters, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Example 9
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and relative humidity of 80% for 30 days, taking out, and scraping surface hypha. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.15% water-based composite organic bactericide, and the bactericide consists of 30 parts of methoxy acrylate, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Example 10
Sterilizing bamboo, soaking in white rot fungus (Phellinus baumii) suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 30 days, taking out, and scraping surface hypha. And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and then performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.15% water-based composite organic bactericide, and the bactericide consists of 30 parts of methoxy acrylate, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Comparative example 1
The bamboo is not treated.
Comparative example 2
And (3) putting the bamboo into boiling water for hot bath for 2 hours, taking out the bamboo, drying the bamboo, and performing pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which consists of 30 parts of methoxy acrylic acid esters, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Comparative example 3
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 30 days, taking out, and scraping surface hypha. And (3) carrying out hot bath on the bamboo in boiling water for 2 hours, taking out the bamboo, and drying to obtain the mildew-proof bamboo.
Comparative example 4
Sterilizing bamboo, soaking in white rot fungus suspension for 3min, taking out bamboo, culturing in PDA culture medium at 28 deg.C and 80% relative humidity for 50 days, taking out, and scraping surface hypha. And (3) carrying out hot bath on the bamboo wood in boiling water for 2 hours, taking out the bamboo wood, drying the bamboo wood, and then carrying out pressure impregnation treatment by using a bactericide to obtain the mildew-proof bamboo wood.
The formula of the bactericide is as follows: the total effective component is 0.3% aqueous composite organic bactericide, which consists of 30 parts of methoxy acrylic acid esters, 40 parts of isothiazolinone and 30 parts of quaternary ammonium salt.
The pressure impregnation process comprises the following steps: the negative pressure is kept for 10min, the positive pressure is 0.6MPa, and the negative pressure is kept for 60 min.
Experimental example 1 mildew resistance of modified bamboo
Aspergillus niger and mixed multi-bacterium environment mildew resistance tests are carried out in examples 3 to 10 and comparative examples 2 and 3 by referring to GB/T18261-2000 test method for preventing mildew and blue-stain bacteria of wood by mildew preventive and American Wood protection Association standard AWPAE 24-06. The control efficacy is calculated as follows:
wherein: RE is the prevention and treatment effect; dt is the average damage value of the medicament treatment sample; do is the average damage value of the control sample. The mildew resistance of the modified bamboo wood is shown in table 1.
TABLE 1 mildew resistance of modified bamboo
The results show that the bamboo wood which is only pretreated by white rot fungi (coriolus versicolor) or soaked by bactericide under pressure has certain improvement of mildew-proof effect, but the surface still has mildew growth after 4 weeks. After the white rot fungi are pretreated for 30 days, the bactericide is pressurized and impregnated, the control efficiency of the bamboo is 100 percent, and the surface is completely free of mould growth. The content of active ingredients of the bactericide is reduced, the control efficiency of bamboo pretreated by the coriolus versicolor is still 100%, and a small amount of mould grows on the surface of the bamboo pretreated by the phanerochaete sporotrichioides, the pleurotus ostreatus and the phellinus baumii after being soaked in the low-concentration bactericide. Namely, the white rot fungi pretreatment can improve the drug loading of the bactericide, wherein the coriolus versicolor pretreatment effect is optimal.
Experimental example 2 influence of pretreatment of white rot fungi on degradation resistance and loss resistance of bamboo fungicides
Taking examples 1-3 and comparative example 2, performing a mildew prevention experiment and an anti-loss experiment, slicing bamboo wood in the thickness direction for 1mm after the experiment is finished, and measuring the effective components of the bactericide in bamboo chips with the thickness of 1mm by using a high performance liquid chromatograph, as shown in fig. 1(a) and 1 (b).
The results show that after the white rot fungi (coriolus versicolor) are coupled with the organic bactericide modified bamboo wood, through a mildew-proof experiment and an anti-loss experiment, the drug-loading rate of effective components in the bamboo wood is obviously higher than that of the bamboo wood modified by only the organic bactericide, so that metabolites secreted by the white rot fungi (coriolus versicolor) can improve the anti-loss performance and the anti-biodegradability of the bactericide, and the mildew-proof aging of the organic bactericide is prolonged.
Experimental example 3 changes in starch content and liquid absorption rate of white rot fungi pretreated bamboo
Measuring the starch content by adopting a spectrophotometer, grinding the mildew-proof bamboo wood of examples 1-3 and comparative examples 1 and 2 into powder, filtering by using hydrochloric acid solution, and dropwise adding KI-I 2 And detecting the absorbance of the reagent at the wavelength of 580nm of an absorption peak to determine the content of the starch. Taking the mildew-proof bamboo wood obtained in the embodiments 1-3 and the comparative example 2, weighing the wet weight mass of the bamboo wood after pressure impregnation, and calculating the liquid absorption rate according to the following formula:
a is the liquid absorption rate, m 1 Is the wet weight, m 0 Is air-dried mass. The results are shown in FIGS. 2(a) and 2 (b).
The results show that the starch content in bamboo wood is gradually reduced along with the increase of the pretreatment time of white rot fungi (coriolus versicolor), and a part of the starch content can be reduced by pressure impregnation and water bath treatment. After the pretreatment of white rot fungi (coriolus versicolor) for 30 days, the starch in the bamboo is completely removed. The imbibition rate increases with the time of white rot fungus (coriolus versicolor) treatment. The pretreatment of the white rot fungi (coriolus versicolor) is proved to effectively reduce the starch content in the bamboo wood and improve the permeability of the bactericide to the bamboo wood.
Experimental example 4 bacteriostatic properties of metabolites of white rot fungi
Detecting metabolites secreted by white rot fungi (coriolus versicolor) and the bacteriostatic activity of the bactericide by a bacteriostatic circle filter paper method. And (3) taking the solution obtained by the hot bath in the comparative example 2, and carrying out an inhibition zone experiment. Taking the water-based composite organic bactericide with the total effective component of 0.15 percent, diluting by 30 times, and carrying out the bacteriostasis zone experiment. Mixing the two solutions in a volume ratio of 1:1, and carrying out a zone of inhibition experiment. The results are shown in FIG. 3.
The result shows that the metabolite secreted by the white rot fungi (coriolus versicolor) has certain bacteriostatic activity, and the bacteriostatic activity of the bactericide can be effectively improved by combining with the organic bactericide, so that the mildew-proof efficiency is enhanced.
Experimental example 5 mechanical properties of modified bamboo
The bending strength of the bamboo materials is tested in examples 3 and 6 and comparative examples 1 and 4 by referring to JGT199-2007 test method for physical and mechanical properties of bamboo materials for buildings. The mechanical properties of the modified bamboo wood are shown in table 2.
TABLE 2 mechanical Properties of modified bamboo
The result shows that the mechanical property of the bamboo material is not obviously changed after the bamboo material is treated by the coriolus versicolor bacteria for 30 days. After 50 days of pretreatment, the mechanical property of the bamboo wood is obviously reduced. The mechanical properties of the bamboos at the early decay stage of the phellinus baumii are obviously damaged, so that the phellinus baumii is not suitable to be used as white-rot fungi for pretreating the bamboos.
While the present invention has been described with reference to the particular illustrative embodiments, it is not to be restricted by the embodiments but only by the appended claims. It will be understood by those skilled in the art that variations and modifications of the embodiments of the present invention can be made without departing from the scope and spirit of the invention.
Claims (10)
1. A bamboo mildew-proof treatment method coupling white rot fungus pretreatment and bactericide impregnation is characterized by comprising the following steps:
s1, preparing a white rot fungus suspension;
s2, pretreating the bamboo wood by adopting the white rot fungus suspension;
s3, performing water bath treatment and drying treatment on the pretreated bamboo wood;
s4, preparing an organic bactericide;
s5, carrying out pressure impregnation on the organic bactericide;
and S6, performing post-treatment on the bamboo wood.
2. The method for mildew-proof bamboo wood by coupling the pretreatment of white rot fungi and the impregnation of a bactericide as claimed in claim 1, wherein the white rot fungi are coriolus versicolor (Trametes versicolor), Phanerochaete chrysosporium (Phanerochaete chrysosporium), Pleurotus ostreatus (Pleurotus ostreatus) or Phellinus baumii (Phellinus baumii).
3. The method for bamboo wood mildew-proof treatment by coupling white rot fungus pretreatment and bactericide impregnation according to claim 1, wherein the S1 comprises:
s11, transferring the white rot fungi from the slant culture medium to a plate filled with a potato agar culture medium;
s12, activating at 28 + -0.2 deg.C and 85% relative humidity;
s13, putting the fungus cake with the diameter of 1.5cm into a beaker, adding 200mL of sterile water, and uniformly stirring by using a stirrer to prepare white rot fungus suspension for later use.
4. The method for bamboo wood mildew-proof treatment by coupling white rot fungus pretreatment and bactericide impregnation according to claim 3, wherein the S2 comprises:
s21, uniformly inoculating the white rot fungi suspension onto bamboo wood in a soaking or spraying mode, wherein the soaking time in the white rot fungi suspension is 2-20 min under the soaking condition; under the condition of adopting the spraying, the spraying amount of the white rot fungus suspension is 0.5 kg-2 kg/m 2 ；
S22, transferring the inoculated bamboo wood into a constant temperature and humidity incubator, and culturing at the temperature of 28 +/-0.2 ℃ and the relative humidity of 85% for 5-50 days.
5. The bamboo mildew-proof treatment method by coupling white rot fungus pretreatment and bactericide impregnation according to claim 4, wherein the soaking time is 2-10 min, or the spraying amount is 0.5 kg-1.5 kg/m 2 And the culture time is 10-30 days.
6. The method for bamboo wood mildew-proof treatment by coupling white rot fungus pretreatment and bactericide impregnation according to claim 4, wherein the S3 comprises:
s31, scraping hypha on the surface of the pretreated bamboo wood;
and S32, placing the mixture into boiling water for hot bath for 0.5-5 hours, taking out the mixture, and drying the mixture at 63 ℃ to constant weight.
7. The bamboo wood mildew-proof treatment method by coupling white rot fungi pretreatment and bactericide impregnation according to claim 1, wherein the organic bactericide is an aqueous composite organic bactericide with a total effective component of 0.1-0.5%, wherein the total effective component represents the total concentration of the organic bactericide.
8. The bamboo mildew-proof treatment method by coupling white rot fungus pretreatment and bactericide impregnation according to claim 7, wherein the organic bactericide comprises 1-30 parts of strobilurins, 1-50 parts of isothiazolinones and 1-40 parts of quaternary ammonium salts.
9. The method for bamboo wood mildew-proof treatment by coupling white rot fungus pretreatment and bactericide impregnation according to claim 1, wherein the S5 comprises: and (3) soaking the organic bactericide into the pretreated bamboo wood, and keeping the vacuum pressure at 0.5-1.5 MPa for 40-60 min.
10. The method for bamboo wood mildew-proof treatment by coupling white rot fungus pretreatment and bactericide impregnation according to claim 1, wherein the S6 comprises: and taking out the bamboo wood subjected to the dipping modification in the S5, and transferring the bamboo wood to a drying kiln for post-fixing treatment, wherein the temperature of the post-fixing treatment is 40-80 ℃.
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