CN115104529A - Gene editing material selfing homozygous management method and information platform - Google Patents
Gene editing material selfing homozygous management method and information platform Download PDFInfo
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- CN115104529A CN115104529A CN202210888557.8A CN202210888557A CN115104529A CN 115104529 A CN115104529 A CN 115104529A CN 202210888557 A CN202210888557 A CN 202210888557A CN 115104529 A CN115104529 A CN 115104529A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q50/00—Systems or methods specially adapted for specific business sectors, e.g. utilities or tourism
- G06Q50/02—Agriculture; Fishing; Mining
Abstract
The invention discloses a gene editing material selfing homozygous management method and an information platform, wherein the method comprises the following steps: based on a gene editing and breeding coding system, utilizing an information management platform to carry out selfing homozygous point seed screening; according to the screening result of the inbred homozygous dibbling, based on a gene editing and breeding coding system, and by utilizing an information management platform, carrying out floating tray dibbling, floating tray seedling raising and molecular sampling in sequence; according to the molecular sampling result, tobacco seedlings edited at the target sites are screened out and transplanted to a greenhouse, and tobacco plant management and seed collection management are carried out by utilizing an information management platform based on a gene editing and breeding coding system. The gene editing material selfing homozygous management method and the information platform provided by the invention are based on a gene editing breeding coding system, and provide support for timely storage, accurate acquisition and efficient traceability of information and data such as seeds, materials and detection through informatization management of multiple operation flows of dibbling, seedling raising, detection screening and seed harvesting related to a selfing homozygous propagation process.
Description
Technical Field
The invention relates to the technical field of crop breeding digital management, in particular to a gene editing material selfing homozygous management method and an information platform.
Background
Genome editing technology is applied to tobacco genetic breeding, wherein a tobacco genome editing method is to transform a plant cell with a DNA encoding a sequence-specific nuclease (such as CRISPR/Cas9), a CRISPR vector is integrated in a plant chromosome, and a plant without CRISPR/Cas9DNA is obtained through progeny separation. The T0 generation editing material plants containing CRISPR vector labels are propagated for one generation, the probability of obtaining the unlabeled homozygote editing material is about 1/16, and for the plants which are not obtained the unlabeled homozygote material in the propagation for one generation, higher generation propagation is needed. The main purpose of the tobacco gene editing material selfing homozygous expanded propagation planting is to obtain a non-label homozygote material, and provide a material basis for gene function research and strain cultivation.
The inbred homozygous propagation process relates to a plurality of operation flows such as dibbling, seedling raising, detection screening, seed harvesting and seed screening, and a gene editing material inbred homozygous management method and an information platform are urgently needed to ensure timely storage, accurate acquisition and efficient traceability of information and data such as seeds, materials and detection, so as to solve the practical problems of large quantity, multiple and long flows and the like of inbred homozygous material propagation.
Disclosure of Invention
The invention aims to provide a gene editing material selfing homozygous management method and an information platform, which are used for solving the problems in the prior art and can provide technical support for efficient traceability and accurate management of tobacco gene editing material selfing homozygous.
The invention provides a gene editing material selfing homozygous management method, which comprises the following steps:
based on a tobacco gene editing and breeding coding system, utilizing an information management platform to carry out selfing homozygous dibbling screening;
according to the screening result of the inbred homozygous dibbling, based on a tobacco gene editing and breeding coding system, and by utilizing an information management platform, carrying out floating tray dibbling, floating tray seedling raising and molecular sampling in sequence;
according to the molecular sampling result, tobacco seedlings with target sites edited are screened out from floating tray tobacco seedlings, the screened tobacco seedlings are transplanted to a greenhouse, and tobacco plant management and seed collection management are carried out after the tobacco seedlings are transplanted by utilizing an information management platform based on a tobacco gene editing and breeding coding system.
The method for managing inbred homozygosity of gene editing material as described above, wherein preferably, the screening of inbred homozygosity seed selection is performed by using an information management platform based on the tobacco gene editing breeding coding system, and specifically comprises:
based on a pre-established tobacco gene editing and breeding coding system and a selfing homozygous dibbling screening principle, an information management platform is utilized to make and issue a selfing homozygous dibbling plan.
The method for managing inbred homozygosity of gene editing material as described above, wherein preferably, the screening of inbred homozygosity seed points by using an information management platform specifically comprises:
based on a pre-established tobacco gene editing and breeding coding system and a selfing homozygous dibbling screening principle, an information management platform is utilized to make and issue a selfing homozygous dibbling plan;
and carrying out selfing homozygous point seed screening according to the selfing homozygous point seed plan.
The method for managing inbred homozygosity of gene editing materials as described above, wherein preferably, the floating tray dibbling, floating tray seedling raising and molecular sampling are sequentially performed by using an information management platform based on a tobacco gene editing breeding coding system according to screening results of inbred homozygosity dibbling, and specifically include:
based on a pre-established tobacco gene editing and breeding coding system, utilizing an information management platform, a mobile operation terminal and a portable printer to sequentially perform floating plate seed counting and floating plate seedling raising;
based on a pre-established tobacco gene editing and breeding coding system, molecular sampling management is carried out by utilizing an information management platform, a mobile operation terminal and a portable printer.
The method for managing inbred homozygosity of gene editing materials as described above, wherein preferably, the floating-tray seed counting and floating-tray seedling raising are performed in sequence by using an information management platform, a mobile operation terminal and a portable printer based on a pre-established tobacco gene editing breeding coding system, and specifically include:
an information management platform is utilized to formulate and issue a floating plate seedling raising task;
selecting a two-dimensional code label of a seed to be dibbled by using a mobile operation terminal;
printing the two-dimensional code label of the selected seed to be dibbled by using a portable printer;
sticking the printed two-dimensional code label of the seeds to be dibbled to a floating plate inserting plate;
and performing floating plate seed counting and floating plate seedling raising by using the two-dimension code label on the floating plate inserting plate.
The method for managing inbred homozygosity of gene editing materials as described above, wherein preferably, the molecular sampling management is performed by using an information management platform, a mobile operation terminal and a portable printer based on a pre-established tobacco gene editing breeding coding system, and specifically comprises:
utilizing an information management platform to formulate and issue a molecular detection sampling task;
generating a sampling table according to the molecular detection sampling task;
manufacturing a sampling tube according to the sampling table;
scanning a two-dimensional code label of the tobacco seedling to be sampled by using a mobile operation terminal;
printing a scanning result of the mobile operation terminal by using a portable printer;
sticking the printed two-dimensional code label of the tobacco seedling to be sampled to the sampling tube;
collecting tobacco seedling leaves corresponding to the two-dimensional codes and placing the tobacco seedling leaves in the sampling pipe;
and (3) sending the sampling tube to carry out molecular detection, collecting molecular detection data in the molecular detection process, and uploading the collected molecular detection data to an information management platform for management.
The method for managing inbreeding and homozygosis of the gene editing material, preferably, the method comprises the following steps of screening out tobacco seedlings edited at target sites from floating tray tobacco seedlings according to molecular sampling results, transplanting the screened tobacco seedlings to a greenhouse, editing breeding coding systems based on tobacco genes, and managing tobacco plants and harvesting by using an information management platform after transplanting, and specifically comprises the following steps:
according to the molecular detection data result on the information management platform, scanning the two-dimensional code of the floating tray tobacco seedling by using the mobile operation terminal, selecting the molecular detection result to display the tobacco seedling edited at the target site, and transplanting the edited tobacco seedling to a greenhouse;
and (4) utilizing the information management platform to manage the tobacco plants after transplanting and to manage the seeds after seed collection.
The method for managing inbred homozygosity of gene editing material as described above, wherein preferably, the tobacco plant management is performed after transplanting and the seed harvest management is performed after seed harvest by using an information management platform, specifically comprising:
bagging the tobacco plant when the tobacco plant is in the bud stage;
after the capsule is mature, placing the two-dimension code label of the tobacco plant and the collected capsule into a seed collecting bag;
drying the capsule in the seed bag to dry the capsule;
scanning the two-dimensional code label in the seed receiving bag by using the mobile operation terminal, and automatically uploading seed scanning information to the information management platform by using the mobile operation terminal;
printing a scanning result of the mobile operation terminal by using a portable printer;
sticking the printed two-dimensional code label of the plant corresponding to the capsule to the seed collecting pipe;
and taking out the seeds in the capsule and putting the seeds into a seed receiving tube with a two-dimensional code label.
The method for managing inbred homozygosity of gene editing material as described above, preferably, the method for managing inbred homozygosity of gene editing material further comprises:
and adjusting the selfing homozygous dibbling plan according to the molecular sampling result and/or the harvest management result.
The invention also provides a gene editing material selfing homozygous management information platform adopting the method, which comprises the following steps:
the information management platform is respectively connected with the coding system management module and the information acquisition module, wherein:
the coding system management module is used for managing the two-dimensional codes of seeds, tobacco seedlings or samples;
the information acquisition module is used for sampling and managing seeds, tobacco seedlings or samples according to the coding management result of the coding system management module in a seed sorting and screening stage, a floating tray seed sorting stage, a floating tray seedling stage and a molecular sampling stage to obtain sampling information corresponding to the seeds, the tobacco seedlings or the samples in different stages, wherein the sampling information comprises at least one of two-dimensional code information, operation information and data information;
the information management platform is used for issuing corresponding selfing homozygous operation instructions to the information acquisition module in a dibbling screening stage, a floating plate dibbling stage, a floating plate seedling raising stage and a molecular sampling stage of selfing homozygous operation according to the coding management result of the coding system management module, and storing and managing sampling information obtained by the information acquisition module in the dibbling screening stage, the floating plate dibbling stage, the floating plate seedling raising stage and the molecular sampling stage of the selfing homozygous operation.
The gene editing material selfing homozygous management information platform as described above, wherein preferably, the gene editing material selfing homozygous management information platform further includes a communication module disposed between the information management platform and the information acquisition module, and the communication module is configured to transmit the sampling information acquired by the information acquisition module to the information management platform, and to issue the work instruction issued by the information management platform to the information acquisition module.
The invention provides a gene editing material selfing homozygous management method and an information platform, aiming at the key step of selfing homozygous in the tobacco gene editing breeding process, the method provides support for timely storage, accurate acquisition and efficient traceability of information and data of seeds, materials, detection and the like through information management of a plurality of operation flows such as dibbling, seedling raising, detection screening, seed harvesting and the like related to the selfing homozygous expanding breeding process based on a tobacco gene editing breeding coding system.
Drawings
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described with reference to the accompanying drawings, in which:
FIG. 1 is a flowchart of an embodiment of a method for selfing homozygous management of gene editing material according to the present invention;
FIG. 2 is a logic diagram of an embodiment of the method for selfing homozygous management of gene editing material according to the present invention;
fig. 3 is a structural block diagram of an embodiment of the gene editing material selfing homozygous management information platform provided by the present invention.
Detailed Description
Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative and is in no way intended to limit the disclosure, its application, or uses. The present disclosure may be embodied in many different forms and is not limited to the embodiments described herein. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art. It should be noted that: the relative arrangement of parts and steps, the composition of materials, numerical expressions and numerical values set forth in these embodiments are to be construed as merely illustrative, and not as limitative, unless specifically stated otherwise.
As used in this disclosure, "first", "second": and the like, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element preceding the word covers the element listed after the word, and does not exclude the possibility that other elements are also covered. "upper", "lower", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
In the present disclosure, when a specific component is described as being located between a first component and a second component, there may or may not be intervening components between the specific component and the first component or the second component. When it is described that a specific component is connected to other components, the specific component may be directly connected to the other components without having an intervening component, or may be directly connected to the other components without having an intervening component.
All terms (including technical or scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs unless specifically defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate.
As shown in fig. 1 and fig. 2, the method for managing inbred homozygosis of a gene editing material provided in this embodiment specifically includes the following steps in an actual execution process:
and S1, editing a breeding coding system based on the tobacco gene, and screening the inbred homozygous dibbling by using an information management platform.
In an embodiment of the method for managing inbred homozygosity of gene editing material according to the present invention, the step S1 may specifically include:
and S11, based on the pre-established tobacco gene editing and breeding coding system and the selfing homozygous dibbling screening principle, making and issuing a selfing homozygous dibbling plan by using an information management platform.
In some embodiments of the present invention, the inbred homozygous point program can be, for example:
firstly, under the condition that the screening condition is that the number of genes is 1, obtaining a seed list edited by a single gene;
then, under the condition that the screening condition is that the type of the gene editing is heterozygous or no editing, obtaining a seed list which is not homozygous for the single gene editing;
thirdly, under the condition that the screening condition is that the generation is T1, obtaining a list of seeds which are not homozygous at the T1 generation of single gene editing;
finally, in the case of the first 100 seeds under the screening conditions, a list of 100 seeds not homozygous for the single gene editing T1 generation was obtained. It should be noted that the present invention does not specifically limit the principle of selecting inbred homozygous dibbling and the corresponding dibbling plan.
And S12, performing inbred homozygous seed selection according to the inbred homozygous seed selection plan.
After a selfing homozygous dibbling plan is made, seeds in the plan are screened.
And S2, according to the screening result of the inbred homozygous dibbling, editing a breeding coding system based on tobacco genes, and sequentially performing tray floating dibbling, tray floating seedling raising and molecular sampling by using an information management platform.
After the seeds are screened through the step S1, the floating-tray seeding, the floating-tray seedling raising and the molecular sampling are sequentially performed using the screened seeds in the step S2. In an embodiment of the method for managing inbred homozygosity of gene editing material according to the present invention, the step S2 may specifically include:
and step S21, based on the pre-established tobacco gene editing and breeding coding system, utilizing an information management platform, a mobile operation terminal (PDA) and a portable printer to perform floating plate seed counting and floating plate seedling raising in sequence.
In an embodiment of the method for managing inbred homozygosity of gene editing material according to the present invention, the step S21 may specifically include:
and S211, utilizing the information management platform to formulate and issue a floating plate seedling raising task.
Wherein, the floating tray seedling raising task issued by the information management platform carries seed coding information,
and step S212, selecting the two-dimensional code label of the seed to be dibbled by using the mobile operation terminal (PDA).
In a particular implementation, the seed code to be printed may be clicked on the PDA.
And step S213, printing the two-dimensional code label of the selected seed to be dibbled by using the portable printer.
After the seed code to be printed is clicked on the PDA, the seed code is printed out on site through a portable printer.
And S214, sticking the printed two-dimensional code label of the seeds to be dibbled to the floating plate inserting plate.
And S215, performing floating plate seed counting and floating plate seedling raising by using the two-dimension code label on the floating plate inserting plate.
And step S22, performing molecular sampling management by using an information management platform, a mobile operation terminal and a portable printer based on a pre-established tobacco gene editing and breeding coding system.
In an embodiment of the method for managing inbred homozygosity of gene editing material according to the present invention, the step S22 may specifically include:
and step S221, utilizing the information management platform to formulate and issue a molecular detection sampling task.
And step S222, generating a sampling table according to the molecular detection sampling task.
And step S223, manufacturing a sampling tube according to the sampling table.
And S224, scanning the two-dimension code label of the tobacco seedling to be sampled by utilizing the mobile operation terminal.
Wherein, the two-dimension code of the tobacco seedling to be sampled corresponds to the two-dimension code label of the dibbling seed in the step S212 and the step S213.
And step S225, printing the scanning result of the mobile operation terminal by using a portable printer.
And step S226, sticking the printed two-dimensional code label of the tobacco seedling to be sampled to the sampling tube.
And S227, collecting tobacco seedling leaves corresponding to the two-dimensional codes and placing the tobacco seedling leaves in the sampling pipe.
Step S228, the sampling tube is sent to carry out molecular detection, molecular detection data are collected in the molecular detection process, and the collected molecular detection data are uploaded to an information management platform for management.
In specific implementation, the sampling tube is sent to the molecule detection mechanism for molecule detection, and the molecule detection data returned by the molecule detection mechanism is uploaded to the information management platform through the communication module.
And S3, screening out tobacco seedlings with target sites edited from the floating tray tobacco seedlings according to the molecular sampling result, transplanting the screened tobacco seedlings to a greenhouse, editing a breeding coding system based on tobacco genes, and performing tobacco plant management and seed collection management after transplanting by using an information management platform.
In an embodiment of the method for managing inbred homozygosity of gene editing material according to the present invention, the step S3 may specifically include:
and S31, scanning the two-dimensional code of the floating tray tobacco seedling by using the mobile operation terminal according to the molecular detection data result on the information management platform, selecting the tobacco seedling with the molecular detection result showing the target site edited, and transplanting the edited tobacco seedling to a greenhouse.
And if the molecular detection result shows editing, transplanting the tobacco seedling.
And step S32, using the information management platform to manage the tobacco plants after transplanting and to manage the seeds after seed collection.
In an embodiment of the method for managing inbred homozygosity of gene editing material according to the present invention, the step S32 may specifically include:
and S321, bagging the tobacco plant when the tobacco plant is in the bud stage.
And S322, after the capsule is mature, putting the two-dimension code label of the tobacco plant and the collected capsule into a seed collecting bag together.
And S323, airing the capsule in the seed receiving bag to dry the capsule.
Step S324, scanning the two-dimension code label in the seed receiving bag by using the mobile operation terminal, and simultaneously, automatically uploading the seed scanning information to the information management platform by using the mobile operation terminal.
And step S325, printing the scanning result of the mobile operation terminal by using a portable printer.
And S326, attaching the printed two-dimensional code label of the plant corresponding to the capsule to the seed collecting pipe.
And S327, taking out the seeds in the capsules and putting the seeds into a seed receiving tube with a two-dimensional code label.
In some embodiments of the present invention, the method for managing selfing homozygosity of gene editing material further comprises:
and S4, adjusting the inbred homozygous dibbling plan according to the molecular sampling result and/or the yield management result.
The gene editing material selfing homozygous management method provided by the embodiment of the invention aims at the key step of selfing homozygous in the tobacco gene editing breeding process, and provides support for timely storage, accurate acquisition and efficient traceability of information and data of seeds, materials, detection and the like through informatization management of a plurality of operation flows such as dibbling, seedling raising, detection screening, seed harvesting and the like related to the selfing homozygous expanding breeding process based on a tobacco gene editing breeding coding system.
Correspondingly, as shown in fig. 3, the present invention further provides a management information platform for inbred homozygosis of gene editing material, comprising:
coding system management module 1, information management platform 2 and information acquisition module 3, information management platform 2 respectively with coding system management module 1 with information acquisition module 3 is connected, wherein:
the coding system management module 1 is used for managing two-dimensional codes of seeds, tobacco seedlings or samples;
the information acquisition module 3 is used for sampling and managing seeds, tobacco seedlings or samples according to the coding management result of the coding system management module 1 in a seed counting screening stage, a floating tray seed counting stage, a floating tray seedling stage and a molecular sampling stage to obtain sampling information corresponding to the seeds, the tobacco seedlings or the samples in different stages, wherein the sampling information comprises at least one of two-dimensional code information, operation information and data information;
the information management platform 2 is used for issuing corresponding selfing homozygous operation instructions to the information acquisition module in a dibbling screening stage, a floating plate dibbling stage, a floating plate seedling stage and a molecular sampling stage of selfing homozygous operation according to the coding management result of the coding system management module, and storing and managing sampling information obtained by the information acquisition module 3 in the dibbling screening stage, the floating plate seedling stage and the molecular sampling stage of the selfing homozygous operation.
Furthermore, the gene editing material selfing homozygous management information platform further comprises a communication module 4 arranged between the information management platform 2 and the information acquisition module 3, wherein the communication module 4 is used for transmitting the sampling information acquired by the information acquisition module 3 to the information management platform 3 and issuing a working instruction issued by the information management platform 2 to the information acquisition module 3.
According to the gene editing material selfing homozygous management information platform provided by the embodiment of the invention, aiming at the key step of selfing homozygous in the tobacco gene editing breeding process, the information platform provides support for timely storage, accurate acquisition and efficient traceability of information and data of seeds, materials, detection and the like through information management of a plurality of operation flows such as dibbling, seedling raising, detection screening, seed harvesting and the like related to the selfing homozygous expanding breeding process based on a tobacco gene editing breeding coding system.
Thus, various embodiments of the present disclosure have been described in detail. Some details that are well known in the art have not been described in order to avoid obscuring the concepts of the present disclosure. It will be fully apparent to those skilled in the art from the foregoing description how to practice the presently disclosed embodiments.
Although some specific embodiments of the present disclosure have been described in detail by way of example, it should be understood by those skilled in the art that the foregoing examples are for purposes of illustration only and are not intended to limit the scope of the present disclosure. It will be understood by those skilled in the art that various changes may be made in the above embodiments or equivalents may be substituted for elements thereof without departing from the scope and spirit of the present disclosure. The scope of the present disclosure is defined by the appended claims.
Claims (10)
1. A gene editing material selfing homozygous management method is characterized by comprising the following steps:
based on a tobacco gene editing and breeding coding system, utilizing an information management platform to carry out selfing homozygous dibbling screening;
according to the screening result of the inbred homozygous dibbling, based on a tobacco gene editing and breeding coding system, and by utilizing an information management platform, carrying out floating tray dibbling, floating tray seedling raising and molecular sampling in sequence;
according to the molecular sampling result, tobacco seedlings with target sites edited are screened out from floating tray tobacco seedlings, the screened tobacco seedlings are transplanted to a greenhouse, and tobacco plant management and seed collection management are carried out after the tobacco seedlings are transplanted by using an information management platform based on a tobacco gene editing and breeding coding system.
2. The method for managing inbred homozygosity of gene editing material according to claim 1, wherein the screening of inbred homozygosity seed selection is performed by using an information management platform based on a tobacco gene editing breeding coding system, and specifically comprises:
based on a pre-established tobacco gene editing and breeding coding system and a selfing homozygous dibbling screening principle, an information management platform is utilized to make and issue a selfing homozygous dibbling plan;
and carrying out selfing homozygous point seed screening according to the selfing homozygous point seed plan.
3. The method for managing inbreeding homozygosity of a gene editing material according to claim 1, wherein the method for managing inbreeding homozygosity according to the screening result of the inbreeding homozygosity dibbling is characterized in that floating tray dibbling, floating tray seedling raising and molecular sampling are sequentially performed by using an information management platform based on a tobacco gene editing breeding coding system, and specifically comprises the following steps:
based on a pre-established tobacco gene editing and breeding coding system, utilizing an information management platform, a mobile operation terminal and a portable printer to sequentially perform floating plate seed counting and floating plate seedling raising;
and (3) performing molecular sampling management by using an information management platform, a mobile operation terminal and a portable printer based on a pre-established tobacco gene editing and breeding coding system.
4. The method for homozygous selfing of gene editing material according to claim 3, wherein the method for performing floating-tray seed-counting and floating-tray seedling-raising in sequence based on the pre-established tobacco gene editing breeding coding system by using the information management platform, the mobile operation terminal and the portable printer comprises:
an information management platform is utilized to formulate and issue a floating plate seedling raising task;
selecting a two-dimensional code label of a seed to be dibbled by using a mobile operation terminal;
printing the two-dimensional code label of the selected seed to be dibbled by a portable printer;
sticking the printed two-dimensional code label of the seeds to be dibbled to a floating plate inserting plate;
and performing floating plate seed counting and floating plate seedling raising by using the two-dimension code label on the floating plate inserting plate.
5. The method for homozygous self-crossing management of gene editing material according to claim 3, wherein the molecular sampling management is performed by using an information management platform, a mobile operation terminal and a portable printer based on a pre-established tobacco gene editing and breeding coding system, and specifically comprises:
utilizing an information management platform to formulate and issue a molecular detection sampling task;
generating a sampling table according to the molecular detection sampling task;
manufacturing a sampling tube according to the sampling table;
scanning a two-dimensional code label of the tobacco seedling to be sampled by using a mobile operation terminal;
printing a scanning result of the mobile operation terminal by using a portable printer;
sticking the printed two-dimensional code label of the tobacco seedling to be sampled to the sampling tube;
collecting tobacco seedling leaves corresponding to the two-dimensional codes and placing the tobacco seedling leaves in the sampling pipe;
and (3) sending the sampling tube to carry out molecular detection, collecting molecular detection data in the molecular detection process, and uploading the collected molecular detection data to an information management platform for management.
6. The method for homozygous self-crossing management of gene editing material according to claim 1, wherein the method comprises the steps of screening tobacco seedlings edited at target sites from floating tray tobacco seedlings according to molecular sampling results, transplanting the screened tobacco seedlings to a greenhouse, editing breeding coding systems based on tobacco genes, and performing tobacco plant management and seed collection management after transplanting by using an information management platform, and specifically comprises the steps of:
according to the molecular detection data result on the information management platform, scanning the two-dimensional code of the floating tray tobacco seedling by using the mobile operation terminal, selecting the molecular detection result to display the tobacco seedling edited at the target site, and transplanting the edited tobacco seedling to a greenhouse;
and (4) utilizing the information management platform to perform tobacco plant management after transplanting and perform seed collection management after seed collection.
7. The method for homozygous selfing of gene editing material according to claim 6, wherein said information management platform is used for tobacco plant management after transplanting and for seed harvest management after seed harvest, and the method specifically comprises:
bagging the tobacco plant when the tobacco plant is in the bud stage;
after the capsule is mature, placing the two-dimension code label of the tobacco plant and the collected capsule into a seed collecting bag;
drying the capsule in the seed bag to dry the capsule;
scanning the two-dimensional code label in the seed receiving bag by using the mobile operation terminal, and automatically uploading seed scanning information to the information management platform by using the mobile operation terminal;
printing a scanning result of the mobile operation terminal by using a portable printer;
sticking the printed two-dimensional code label of the plant corresponding to the capsule to the seed collecting pipe;
and taking out the seeds in the capsule and putting the seeds into a seed receiving tube with a two-dimensional code label.
8. The method for managing selfing homozygosity of gene editing material according to claim 1, further comprising:
and adjusting the selfing homozygous dibbling plan according to the molecular sampling result and/or the harvest management result.
9. A gene editing material selfing homozygous management information platform is characterized by comprising: the information management platform is respectively connected with the coding system management module and the information acquisition module, wherein:
the coding system management module is used for managing the two-dimensional codes of seeds, tobacco seedlings or samples;
the information acquisition module is used for sampling and managing seeds, tobacco seedlings or samples according to the coding management result of the coding system management module in a seed sorting and screening stage, a floating tray seed sorting stage, a floating tray seedling stage and a molecular sampling stage to obtain sampling information corresponding to the seeds, the tobacco seedlings or the samples in different stages, wherein the sampling information comprises at least one of two-dimensional code information, operation information and data information;
the information management platform is used for issuing corresponding selfing homozygous operation instructions to the information acquisition module in a dibbling screening stage, a floating plate dibbling stage, a floating plate seedling raising stage and a molecular sampling stage of selfing homozygous operation according to the coding management result of the coding system management module, and storing and managing sampling information obtained by the information acquisition module in the dibbling screening stage, the floating plate dibbling stage, the floating plate seedling raising stage and the molecular sampling stage of the selfing homozygous operation.
10. The gene editing material selfing homozygous management information platform of claim 9, further comprising a communication module disposed between the information management platform and the information collection module, wherein the communication module is configured to transmit the sampling information obtained by the information collection module to the information management platform, and to send work instructions sent by the information management platform to the information collection module.
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