CN115093955A - PCR amplification detection device based on micro-fluidic chip - Google Patents
PCR amplification detection device based on micro-fluidic chip Download PDFInfo
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The invention relates to a PCR amplification detection device based on a microfluidic chip, which comprises the microfluidic chip integrated with a PCR amplification module and a detection mechanism, wherein the microfluidic chip is provided with a plurality of detection micro-pipelines; the detection mechanism comprises a centrifugal motor module, a temperature control module, a light path switching control module and a fluorescence detection control module; the centrifugal motor module comprises a chip fixing piece, a centrifugal motor, a moving bottom plate, a moving toothed belt and a left-right movement motor, the micro-fluidic chip is fixedly installed on the chip fixing piece, the centrifugal motor is installed at the bottom end of the micro-fluidic chip, the chip fixing piece is installed on the moving bottom plate, the moving toothed belt is installed on one side of the moving bottom plate and drives the moving toothed belt through the left-right movement motor to drive the micro-fluidic chip to move to the positions of the temperature control module, the light path switching control module and the fluorescence detection control module. The detection device can realize multi-item detection of a single sample by applying the microfluidic chip technology, can improve the detection efficiency and reduce the detection time.
Description
Technical Field
The invention relates to the technical field of biomedical detection and diagnosis, in particular to a PCR amplification detection device based on a microfluidic chip.
Background
The microfluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes on a micron-scale chip to automatically complete the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine and the like, the method has been developed into a new research field crossing the disciplines of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like.
Due to unique technical characteristics and advantages, the micro-fluidic chip technology is increasingly paid attention by researchers in various countries around the world, and has potential application prospects in various fields such as life science, medical diagnosis, food safety, animal and plant inspection and quarantine and the like. For the biomedical diagnosis field, the microfluidic chip has the following significant advantages: through the automatic and running-type working mode, the sample processing time is greatly shortened, the detection efficiency is improved, the consumption of reaction reagents and samples is reduced, and finally, the automatic, low-cost and intelligent rapid medical detection is realized.
Polymerase Chain Reaction (PCR) is a method of amplifying a DNA fragment as a template to a sufficient amount for structural and functional analysis in the presence of DNA polymerase and nucleotide substrates. The PCR detection method has very important significance in clinically and rapidly diagnosing bacterial infectious diseases and the like. As a detection method with high sensitivity and high specificity, a nucleic acid diagnostic analysis method based on the PCR technology is one of the core technologies in molecular diagnosis. In recent years, nucleic acid diagnostic analysis methods based on microfluidic chips have also received much attention. The development of the PCR amplification detection system based on the microfluidic chip can improve the PCR amplification efficiency, reduce the detection time, and reduce the detection cost and risk by means of reducing the reagent dosage, reducing manual operation and the like.
The microfluidic technology is combined with PCR amplification detection to construct an integrated nucleic acid amplification detection system, which has important practical significance for further improving the nucleic acid diagnosis and analysis efficiency and realizing the automation of the whole nucleic acid diagnosis and analysis process.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides the PCR amplification detection device based on the microfluidic chip, and the detection device can realize multi-item detection of a single sample by applying the microfluidic chip technology, can improve the detection efficiency, reduce the detection time, and is beneficial to popularization and application of the detection device in the technical fields of biomedical detection, diagnosis and the like.
A further aim of this application is through setting the heating module to independent temperature control module, to the detection project of difference, can realize the control of different temperatures and make each detection project not influenced each other on same micro-fluidic chip, guarantee detection effect.
In order to realize the purpose of the invention, the invention adopts the following technical scheme: a PCR amplification detection device based on a microfluidic chip comprises the microfluidic chip integrated with a PCR amplification module and a detection mechanism, wherein the microfluidic chip is provided with a plurality of detection micro-pipelines; the detection mechanism comprises a centrifugal motor module for realizing sample introduction of the sample of the microfluidic chip, a temperature control module for controlling the detection temperature of the microfluidic chip, and a light path switching control module and a fluorescence detection control module which can be matched with each other to finish fluorescence detection of the microfluidic chip at different wave bands; the centrifugal motor module comprises a chip fixing piece, a centrifugal motor, a movable bottom plate, a movable toothed belt and a left-right movement motor, wherein the micro-fluidic chip is fixedly installed on the chip fixing piece, the centrifugal motor is installed at the bottom end of the micro-fluidic chip and used for controlling the realization of sample introduction of the micro-fluidic chip, the chip fixing piece is installed on the movable bottom plate, the movable toothed belt is installed on one side of the movable bottom plate and driven by the left-right movement motor to move the toothed belt and further drive the micro-fluidic chip to the temperature control module, the light path switching control module and the fluorescence detection control module move at the position.
As a preferable aspect of the present invention, the microfluidic chip is disposed in a disc shape, and the plurality of detection microchannels are disposed equidistantly along a circumferential direction of the microfluidic chip.
As a preferable aspect of the present invention, the centrifugal motor module further includes a chip clamping member, the chip clamping member is mounted on a periphery of the microfluidic chip, and the chip clamping member is matched with the chip fixing member to fix the microfluidic chip.
As a preferred scheme of the present invention, the temperature control module includes an up-and-down movement support, a heat sink, a heating and cooling plate and an up-and-down movement motor, the heating and cooling plate is disposed on a side of the heat sink, the up-and-down movement support is mounted below the heat sink and drives the up-and-down movement support through the up-and-down movement motor to complete up-and-down movement control of the detection mechanism and a circular reaction of the PCR amplification module.
As a preferable aspect of the present invention, the temperature control module further includes a cooling fan, and the cooling fan is mounted above the heat sink.
As a preferable embodiment of the present invention, the fluorescence detection control module is mounted on the top of the optical path switching control module.
As a preferred scheme of the present invention, the optical path switching control module includes an optical path switching motor for switching an optical path to implement optical path detection of different wave bands.
As a preferable aspect of the present invention, the detection device further includes a detection optical fiber, and the detection optical fiber is mounted below the heat sink.
As a preferable scheme of the present invention, the detection apparatus further includes a bottom plate, and the up-and-down moving pillar, the left-and-right moving motor, the up-and-down moving motor, and the optical path switching motor are all mounted on the bottom plate.
In a preferred embodiment of the present invention, the bottom plate has a planar surface.
Compared with the prior art, the invention has the beneficial effects that: the PCR amplification detection device based on the microfluidic chip can realize multi-item detection of a single sample by arranging the microfluidic chip comprising a plurality of detection microchannels and the detection mechanism and applying the microfluidic technology, can improve the detection efficiency and reduce the detection time, and the internal PCR amplification reaction is only carried out in the microfluidic chip and cannot pollute the external environment.
Furthermore, the sample injection of the microfluidic chip is realized by utilizing the centrifugal motor module and controlling the rotation of the centrifugal motor in the centrifugal motor module, so that the detection effect is ensured.
Furthermore, the invention utilizes the independently arranged temperature control modules aiming at different detection items to realize the control of different temperatures on the same microfluidic chip without influencing each other.
Furthermore, the invention can complete the fluorescence detection of different wave bands by utilizing the matching of the light path switching control module and the fluorescence detection control module, the whole process is safe and pollution-free, and the use experience is enhanced.
Drawings
FIG. 1 is a schematic diagram of a PCR amplification detection apparatus based on a microfluidic chip according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a PCR amplification detection apparatus based on a microfluidic chip in an embodiment of the present invention.
Reference numerals: 1. a chip fixing member; 2. a microfluidic chip; 3. a centrifugal motor; 4. moving the base plate; 5. moving the toothed belt; 6. a vertical movement pillar; 7. a base plate; 8. a fluorescence detection control module; 9. an optical path switching control module; 10. detecting the optical fiber; 11. an optical path switching motor; 12. a left-right movement motor; 13. a cooling fan; 14. a heat sink; 15. heating the refrigeration sheet; 16. a vertical movement motor; 17. a chip clamp.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
As shown in fig. 1 to 2, a PCR amplification detection apparatus based on a microfluidic chip includes a microfluidic chip 2 integrated with a PCR amplification module and a detection mechanism, and in order to realize multi-item detection of a single sample, the microfluidic chip 2 is provided with a plurality of detection microchannels. The detection mechanism comprises a centrifugal motor module for realizing sample introduction of the sample of the microfluidic chip 2, a temperature control module for controlling the detection temperature of the microfluidic chip 2, and a light path switching control module 9 and a fluorescence detection control module 8 which can be matched with the microfluidic chip 2 to complete fluorescence detection at different wave bands. The centrifugal motor module comprises a chip fixing piece 1, a centrifugal motor 3, a movable bottom plate 4, a movable toothed belt 5 and a left-right movement motor 12, in order to ensure the stability of the microfluidic chip 2 in the detection process and avoid the sample from spilling, the microfluidic chip 2 is fixedly installed on the chip fixing part 1, the centrifugal motor 3 is installed at the bottom end of the microfluidic chip 2, the centrifugal motor 3 drives the chip fixing part 1 and drives the micro-fluidic chip 2 to rotate so as to realize sample introduction of the micro-fluidic chip 2, the chip fixing member 1 is mounted on the movable bottom plate 4 capable of moving left and right, the movable cog belt 5 is mounted on one side of the movable bottom plate 4 and drives the movable cog belt 5 through the left and right movement motor 12 so as to drive the micro-fluidic chip 2 to move to the positions of the temperature control module, the optical path switching control module 9 and the fluorescence detection control module 8.
The PCR amplification detection device based on the microfluidic chip can realize multi-item detection of a single sample by arranging the microfluidic chip 2 comprising a plurality of detection micro-pipelines and the detection mechanism and applying the microfluidic technology, can improve the detection efficiency and reduce the detection time, and the internal PCR amplification reaction is only carried out in the microfluidic chip and cannot pollute the external environment.
In order to facilitate the detection sample on the micro-fluidic chip 2 to enter the detection micro-pipeline under the action of the centrifugal motor 3, the micro-fluidic chip 2 is arranged in a disc shape, and the detection micro-pipelines are arranged along the circumferential direction of the micro-fluidic chip 2 at equal intervals, so that the detection sample on the micro-fluidic chip 2 is equally divided, and the accuracy of the detection result of each detection item is ensured.
In order to further ensure the stability of the microfluidic chip 2, a chip clamping member 17 is further disposed in the centrifugal motor module, and the chip clamping member 17 is mounted on the periphery of the microfluidic chip 2 and cooperates with the chip fixing member 1 to fix the microfluidic chip 2. The chip clamping members 17 can be arranged in a plurality of numbers, and are equidistantly distributed along the periphery of the disc-shaped micro-fluidic chip 2, so that the stress uniformity of the micro-fluidic chip 2 is ensured, and the probability of uneven distribution of the detection samples in the detection micro-pipeline is reduced, namely, the detection samples in some detection micro-pipelines are more or overflow, but the detection samples in some detection micro-pipelines are few or even none, so that the purpose of multi-item detection of a single sample in the invention is difficult to realize.
According to the invention, aiming at different detection items, the independent temperature control modules are arranged, so that different temperatures can be controlled on the same microfluidic chip 2 without mutual influence. The temperature control module comprises an up-and-down movement support 6, a radiating fin 14, a heating and refrigerating piece 15 and an up-and-down movement motor 16, wherein the heating and refrigerating piece 15 is arranged on the side edge of the radiating fin 14, the up-and-down movement support 6 is arranged below the radiating fin 14, and the up-and-down movement support 6 is driven by the up-and-down movement motor 16 to complete up-and-down movement control of the detection mechanism and a circular reaction of the PCR amplification module.
In order to further reduce the influence of the temperature on the microfluidic chip 2, the temperature control module further includes a cooling fan 13, and the cooling fan 13 is installed above the heat sink 14.
The fluorescence detection control module 8 is installed on the top of the optical path switching control module 9, and the optical path switching control module 9 includes an optical path switching motor 11 for switching optical paths to realize optical path detection of different wave bands. The detection device can complete the fluorescence detection of different wave bands by utilizing the matching of the light path switching control module 9 and the fluorescence detection control module 8, the whole process is safe and pollution-free, and the use experience is enhanced. The detection device also comprises a detection optical fiber 10, and the detection optical fiber 10 is arranged below the heat sink 14.
In order to ensure the stability of the whole detection device and further ensure the detection effect, the detection device of the invention is further provided with a bottom plate 7, and the up-and-down movement support 6, the left-and-right movement motor 12, the up-and-down movement motor 16 and the light path switching motor 11 are all arranged on the bottom plate 7. Further, the surface of the bottom plate 7 is flat for enhancing stability.
The PCR amplification detection device based on the microfluidic chip in the embodiment of the invention can realize multi-item detection of a single sample by arranging the microfluidic chip 2 comprising a plurality of detection micro-pipelines and a detection mechanism and applying the microfluidic technology, can improve the detection efficiency and reduce the detection time, and can only carry out the internal PCR amplification reaction in the microfluidic chip without polluting the external environment; the sample injection of the microfluidic chip 2 is realized by utilizing the centrifugal motor module and controlling the rotation of the centrifugal motor 3 in the centrifugal motor module, so that the detection effect is ensured; aiming at different detection items, the independently arranged temperature control modules are utilized to realize the control of different temperatures on the same microfluidic chip 2 without influencing each other; fluorescence detection of different wave bands can be completed by matching the light path switching control module 9 with the fluorescence detection control module 8, the whole process is safe and pollution-free, and the use experience is enhanced.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention; thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Although the reference numerals in the figures are used more here: 1. a chip fixing member; 2. a microfluidic chip; 3. a centrifugal motor; 4. moving the base plate; 5. moving the toothed belt; 6. an up-and-down movement support; 7. a base plate; 8. a fluorescence detection control module; 9. an optical path switching control module; 10. detecting the optical fiber; 11. an optical path switching motor; 12. a left-right movement motor; 13. a cooling fan; 14. a heat sink; 15. heating the refrigeration sheet; 16. an up-and-down movement motor; 17. chip clamping, etc., without excluding the possibility of using other terms. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed as being without limitation to any additional limitations that may be imposed by the spirit of the present invention.
Claims (10)
1. The PCR amplification detection device based on the microfluidic chip is characterized by comprising the microfluidic chip (2) integrated with a PCR amplification module and a detection mechanism, wherein the microfluidic chip (2) is provided with a plurality of detection micro-pipelines; the detection mechanism comprises a centrifugal motor module for realizing sample introduction of the micro-fluidic chip (2), a temperature control module for controlling the detection temperature of the micro-fluidic chip (2), and a light path switching control module (9) and a fluorescence detection control module (8) which can be matched with each other to complete fluorescence detection of the micro-fluidic chip (2) at different wave bands; the centrifugal motor module comprises a chip fixing piece (1), a centrifugal motor (3), a moving bottom plate (4), a moving toothed belt (5) and a left-right movement motor (12), wherein the micro-fluidic chip (2) is fixedly installed on the chip fixing piece (1), the centrifugal motor (3) is installed at the bottom end of the micro-fluidic chip (2) and used for controlling and realizing the sample introduction of the micro-fluidic chip (2), the chip fixing piece (1) is installed on the moving bottom plate (4), the moving toothed belt (5) is installed on one side of the moving bottom plate (4) and passes through the left-right movement motor (12) for driving the moving toothed belt (5) and then driving the micro-fluidic chip (2) to the temperature control module, the light path switching control module (9) and the fluorescence detection control module (8) are moved at the position.
2. The microfluidic chip-based PCR amplification detection apparatus according to claim 1, wherein the microfluidic chip (2) is disposed in a disc shape, and a plurality of detection microchannels are disposed at equal intervals along a circumference of the microfluidic chip (2).
3. The PCR amplification detecting device based on the micro-fluidic chip as claimed in claim 2, wherein the centrifugal motor module further comprises a chip clamping member (17), the chip clamping member (17) is mounted on the periphery of the micro-fluidic chip (2) and is matched with the chip fixing member (1) to fix the micro-fluidic chip (2).
4. The PCR amplification detection device based on the microfluidic chip according to claim 3, wherein the temperature control module comprises an up-and-down movement pillar (6), a heat sink (14), a heating and cooling plate (15) and an up-and-down movement motor (16), the heating and cooling plate (15) is arranged on a side of the heat sink (14), the up-and-down movement pillar (6) is installed below the heat sink (14) and the up-and-down movement pillar (6) is driven by the up-and-down movement motor (16) to complete up-and-down movement control of the detection mechanism and cyclic reaction of the PCR amplification module.
5. The PCR amplification detection device based on the microfluidic chip according to claim 4, wherein the temperature control module further comprises a cooling fan (13), and the cooling fan (13) is installed above the heat sink (14).
6. The microfluidic chip-based PCR amplification detection device according to claim 5, wherein the fluorescence detection control module (8) is mounted on top of the optical path switching control module (9).
7. The PCR amplification detection device based on the microfluidic chip according to claim 6, wherein the optical path switching control module (9) comprises an optical path switching motor (11) for switching optical paths to realize detection of optical paths of different wave bands.
8. The microfluidic chip-based PCR amplification detection device according to claim 7, further comprising a detection optical fiber (10), wherein the detection optical fiber (10) is mounted below the heat sink (14).
9. The PCR amplification detecting device based on the microfluidic chip according to claim 8, further comprising a bottom plate (7), wherein the up-and-down moving pillar (6), the left-and-right moving motor (12), the up-and-down moving motor (16) and the light path switching motor (11) are all mounted on the bottom plate (7).
10. The microfluidic chip-based PCR amplification detection device according to claim 9, wherein the surface of the bottom plate (7) is planar.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111312747.7A CN115093955A (en) | 2021-11-08 | 2021-11-08 | PCR amplification detection device based on micro-fluidic chip |
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| CN202111312747.7A CN115093955A (en) | 2021-11-08 | 2021-11-08 | PCR amplification detection device based on micro-fluidic chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116286337A (en) * | 2023-05-09 | 2023-06-23 | 苏州思迈德生物科技有限公司 | Molecular diagnosis equipment suitable for micro-fluidic chip |
| TWI831393B (en) * | 2022-09-27 | 2024-02-01 | 國立臺灣大學 | Pcr detection device and system |
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2021
- 2021-11-08 CN CN202111312747.7A patent/CN115093955A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI831393B (en) * | 2022-09-27 | 2024-02-01 | 國立臺灣大學 | Pcr detection device and system |
| CN116286337A (en) * | 2023-05-09 | 2023-06-23 | 苏州思迈德生物科技有限公司 | Molecular diagnosis equipment suitable for micro-fluidic chip |
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