CN115078312A - Sample detection light path box, light intensity adjusting method and storage medium - Google Patents
Sample detection light path box, light intensity adjusting method and storage medium Download PDFInfo
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Abstract
The embodiment of the invention provides a sample detection light path box, wherein a main control board is used for sending a starting instruction to a driving circuit when receiving a test instruction; the driving circuit is used for driving the excitation light source to emit light so as to excite the sample to be detected to emit emergent light; the photoelectric sensor is used for converting an optical signal of the emergent light into an electric signal and transmitting the electric signal to the main control board; the main control board is also used for judging whether the intensity of the electric signal is within a preset signal intensity range, and if not, generating a resistance adjusting instruction according to the signal intensity of the electric signal and sending the resistance adjusting instruction to the driving circuit; the driving circuit is also used for adjusting the resistance in the driving circuit according to the resistance adjusting instruction so as to adjust the light intensity of the excitation light source. The signal intensity of the emergent light emitted after the concentrations of the samples to be detected with different concentrations are excited by the same excitation light source is different, the light intensity of the excitation light source can be adapted to the concentration of the samples to be detected by changing the light intensity of the excitation light source, the electric signal is prevented from being too weak or too strong, and the accuracy of the detection result is improved.
Description
Technical Field
The invention relates to the technical field of sample detection, in particular to a sample detection light path box, a light intensity adjusting method and a storage medium.
Background
The immunoassay is a detection system based on the photoelectric detection principle, and the technology has the advantages of high sensitivity, simple operation method, rapidness, high efficiency and the like, and is widely applied to medical detection.
The light path box of the immunity detection analyzer is a device capable of emitting ultraviolet light, converging parallel light, reflecting light and receiving the amount of emitted light, and in the existing light path box, the detection result of a sample to be detected is greatly influenced by the light intensity, possibly causing the problem of low test accuracy.
Disclosure of Invention
The invention provides a sample detection light path box, which aims to solve the problem that the existing light path box-based detection result is greatly influenced by light intensity and possibly causes low test accuracy.
In a first aspect, the present invention provides a sample detection light path box, including a main control board, a driving circuit, an excitation light source, and a photoelectric sensor, where the main control board is connected to the driving circuit and the photoelectric sensor, respectively, and the driving circuit is electrically connected to the excitation light source;
the main control board is used for sending a starting instruction to the driving circuit when receiving a test instruction;
the driving circuit is used for driving the excitation light source to emit light so as to excite the sample to be detected to emit emergent light;
the photoelectric sensor is used for converting an optical signal of the emergent light into an electric signal and sending the electric signal to the main control board;
the main control board is also used for judging whether the intensity of the electric signal is within a preset signal intensity range, and if not, generating a resistance adjusting instruction according to the signal intensity of the electric signal and sending the resistance adjusting instruction to the driving circuit;
the driving circuit is further used for adjusting the resistance in the driving circuit according to the resistance adjusting instruction so as to adjust the light intensity of the excitation light source.
In a second aspect, the present invention provides a light intensity adjusting method applied to the sample detection optical path cartridge according to the first aspect, the light intensity adjusting method comprising:
when a test instruction is received, driving an excitation light source to emit light so as to excite a sample to be tested to emit emergent light;
converting the optical signal of the emergent light into an electrical signal;
and judging whether the signal intensity of the electric signal is within a preset signal intensity range, and if not, adjusting the resistance in the driving circuit according to the signal intensity of the electric signal so as to adjust the light intensity of the excitation light source.
In a third aspect, the present invention provides a computer-readable storage medium storing computer instructions for causing a processor to execute the method for adjusting light intensity according to the second aspect of the present invention.
The embodiment of the invention provides a sample detection light path box, which comprises a main control board, a driving circuit, an excitation light source and a photoelectric sensor, wherein the main control board is respectively connected with the driving circuit and the photoelectric sensor; the main control board is used for sending a starting instruction to the driving circuit when receiving the test instruction; the driving circuit is used for driving the excitation light source to emit light so as to excite the sample to be detected to emit emergent light; the photoelectric sensor is used for converting an optical signal of the emergent light into an electric signal and transmitting the electric signal to the main control board; the main control board is also used for judging whether the intensity of the electric signal is within a preset signal intensity range, and if not, generating a resistance adjusting instruction according to the signal intensity of the electric signal and sending the resistance adjusting instruction to the driving circuit; the driving circuit is also used for adjusting the resistance in the driving circuit according to the resistance adjusting instruction so as to adjust the light intensity of the excitation light source. The signal intensity of the emergent light emitted after the concentration of the sample to be detected with different concentrations is excited by the same excitation light source is different, the light intensity of the excitation light source can be adapted to the concentration of the sample to be detected by changing the light intensity of the excitation light source, the signal intensity of the electric signal emitted by the sample to be detected is within a preset intensity range, the phenomenon that the electric signal is difficult to detect when the electric signal is too weak or the detection error is larger or the electric signal is too strong, signal distortion occurs is avoided, and the accuracy of the detection result can be improved.
It should be understood that the statements in this section are not intended to identify key or critical features of the embodiments of the present invention, nor are they intended to limit the scope of the invention. Other features of the present invention will become apparent from the following description.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
Fig. 1 is a block diagram of a sample detection optical path box according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a driving circuit according to an embodiment of the present invention;
FIG. 3 is a block diagram of another sample detection optics box according to an embodiment of the present invention;
FIG. 4 is a block diagram of a sample detection optical path box according to an embodiment of the present invention;
FIG. 5 is a schematic diagram illustrating a comparison of signals with different amplification factors according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of a light intensity adjustment process provided by an embodiment of the present invention;
fig. 7 is a flowchart of a light intensity adjusting method according to a second embodiment of the present invention.
Detailed Description
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, shall fall within the protection scope of the present invention.
Example one
Fig. 1 is a block diagram of a sample detection optical path box according to an embodiment of the present invention, which is applicable to a case where light intensity of an excitation light source is adjusted during detection of a sample, as shown in fig. 1, the sample detection optical path box includes a main control board 10, a driving circuit 20, an excitation light source 30, and a photosensor 40, the main control board 10 is connected to the driving circuit 20 and the photosensor 40, respectively, and the driving circuit 20 is electrically connected to the excitation light source 30.
The main control board is used for sending a starting instruction to the driving circuit when receiving the test instruction.
The detection light path box can be provided with a start detection switch, when the start detection switch is triggered, the start detection switch generates a test instruction light and sends the test instruction light to the main control board, and the test instruction can also be an instruction sent to the main control board by other terminals, for example, a computer where a tester is located.
When the main control board receives the test instruction, a starting instruction is generated according to preset test parameters and is sent to the driving circuit, and the test parameters comprise resistance, current and the like of the driving circuit.
The driving circuit is used for driving the excitation light source to emit light so as to excite the sample to be detected to emit emergent light.
When the driving circuit receives the starting instruction, elements in the driving circuit can be set according to the testing parameters in the starting instruction, so that the electrical parameters of the driving circuit accord with the preset testing parameters, namely the light intensity of the excitation light source electrically connected with the driving circuit is the preset light intensity, when the excitation light source irradiates the sample to be tested, the sample to be tested is excited to emit emergent light, and the emergent light comprises reflected light and emitted light. Generally, the wavelength band of the excitation light source can be selected according to the type of the sample to be detected, for example, for a fluorescence sample, the wavelength band of the excitation light source is commonly 365nm, 470nm, 685nm, and then, according to the wavelength band of the emitted light, for example, under the irradiation of the excitation light source with the 365nm wavelength band, the fluorescence sample is excited to generate fluorescence with the wavelength band of 615 nm.
The photoelectric sensor is used for converting the optical signal of the emergent light into an electrical signal and sending the electrical signal to the main control board. The main control board is also used for judging whether the intensity of the electric signal is within a preset signal intensity range, and if not, generating a resistance adjusting instruction according to the signal intensity of the electric signal and sending the resistance adjusting instruction to the driving circuit.
The photoelectric sensor is provided with a photoresistor which can sense the light and shade change of light so as to output an electric signal. The photoelectric sensor can be a photoelectric tube, a photoresistor or a phototriode and the like. The intensity of the electric signal indicates the concentration of the sample to be detected, and/or the light intensity of the excitation light source, if the electric signal is too weak, the intensity of the electric signal detected by the photoelectric sensor is very unstable due to the limited sensitivity of the photoelectric sensor, that is, the obtained detection result of the sample to be detected is low in accuracy, if the electric signal is too strong, the phenomena of waveform saturation, distortion and the like may occur during processing of the electric signal, and the obtained detection result of the sample to be detected is also low in accuracy. For example, when the minimum voltage signal value detected by the photosensor is 0.1mV and the electrical signal corresponding to the actual optical signal is only 0.03mV, the voltage signal value detected by the photosensor is 0mV, i.e., the electrical signal cannot be detected and a detection error occurs.
Therefore, if the electric signal is too weak or too strong, the intensity of the electric signal can be changed by changing the light intensity of the excitation light source under the condition that the concentration of the sample to be detected is set, so as to improve the accuracy of sample detection. Specifically, a preset signal intensity range and resistance adjustment information are stored in a storage unit in the main control board, when the main control board receives an electric signal, whether the intensity of the electric signal is within the preset signal intensity range can be judged, and if not, a resistance adjustment instruction is generated according to the signal intensity of the electric signal and the resistance adjustment information and is sent to the driving circuit.
The driving circuit is also used for adjusting the resistance in the driving circuit according to the resistance adjusting instruction so as to adjust the light intensity of the excitation light source.
The resistance adjusting instruction may include resistance values of resistors, the driving circuit may include variable resistors, when the drive circuit receives the resistance adjusting instruction, the variable resistor is adjusted according to the resistance value, to regulate the output current of the driving circuit, the light intensity of the excitation light source is in direct proportion to the current flowing through the excitation light source, further, the light intensity of the excitation light source can be adjusted, and further, different light intensities are irradiated on the sample to be detected with the set concentration, the emergent light of the sample is changed, the obtained electric signals are different, the brightness of emergent light emitted by the sample to be measured and the signal intensity of the electric signal collected by the photoelectric sensor are in direct proportion to the light intensity of the excitation light source, the signal intensity of the electric signal can be adjusted, so that the signal intensity of the electric signal is within a preset signal intensity range, subsequent data processing is facilitated, a detection result is obtained, and the detection accuracy is improved.
The embodiment of the invention provides a sample detection light path box, which comprises a main control board, a driving circuit, an excitation light source and a photoelectric sensor, wherein the main control board is respectively connected with the driving circuit and the photoelectric sensor, and the driving circuit is electrically connected with the excitation light source; the main control board is used for sending a starting instruction to the driving circuit when receiving the test instruction; the driving circuit is used for driving the excitation light source to emit light so as to excite the sample to be detected to emit emergent light; the photoelectric sensor is used for converting an optical signal of the emergent light into an electric signal and transmitting the electric signal to the main control board; the main control board is also used for judging whether the intensity of the electric signal is within a preset signal intensity range, and if not, generating a resistance adjusting instruction according to the signal intensity of the electric signal and sending the resistance adjusting instruction to the driving circuit; the driving circuit is also used for adjusting the resistance in the driving circuit according to the resistance adjusting instruction so as to adjust the light intensity of the excitation light source. The signal intensity of the emergent light that sends after it is aroused by same excitation light source of the sample concentration that awaits measuring of different concentration is also different, through the light intensity to changing excitation light source, make excitation light source's light intensity can adapt to with the concentration of the sample that awaits measuring, the signal intensity of the signal of telecommunication that makes the sample that awaits measuring send is in predetermineeing intensity range, it is difficult to detected or detection error is great when avoiding the signal of telecommunication too weak, and the signal distortion appears when avoiding the signal of telecommunication too strong, can improve the accuracy nature of testing result.
In an alternative embodiment of the present invention, as shown in fig. 1, a phototransistor 50 is disposed beside the excitation light source 30, and the phototransistor 50 is connected to the main control board 10.
The phototriode is used for detecting the light intensity of the excitation light source and sending the light intensity to the main control board after the drive circuit drives the excitation light source to emit light, and the main control board is used for generating a resistance adjusting instruction according to the light intensity difference and sending the resistance adjusting instruction to the drive circuit when the light intensity of the excitation light source has the light intensity difference with the preset initial light intensity. Accordingly, the driving circuit can adjust the resistance in the driving circuit according to the resistance adjusting instruction to adjust the light intensity of the excitation light source.
The preset initial light intensity is the standard light intensity when the excitation light source is started to work, and can be set to be the light intensity suitable for most types of samples to be detected. Because the light intensity of excitation light source receives external detection environment, temperature, voltage supply and the ageing influence of lamp pearl, even same machine sets up the excitation light source with the initial setting parameter that corresponds with initial light intensity, the light intensity of excitation light source also need not conform to with initial light intensity. Therefore, when the light path box is started to work, the light intensity of the excitation light source can be detected through the phototriode and sent to the main control board, the main control board compares the light intensity of the excitation light source with the preset initial light intensity, if the light intensity difference exists, the driving circuit is controlled to adjust the light intensity of the excitation light source, and the adjusting process is circulated until the light intensity of the excitation light source is consistent with the preset initial light intensity. The above processes can be circulated within a preset time period of the opening work of the light path box to correct the light intensity of the excitation light source. In addition, the condition for generating the resistance adjustment instruction may be further set to generate the resistance adjustment instruction according to a light intensity difference when the light intensity of the excitation light source has a light intensity difference from a preset initial light intensity and the light intensity difference is outside a preset light intensity difference range.
Wherein, through opening the during operation at the light path box and calibrating excitation light source's light intensity, can reduce external detection environment, temperature, voltage supply and the ageing influence to excitation light source of lamp pearl, improve detection accuracy and stability.
In an alternative embodiment of the present invention, as shown in fig. 2, the driving circuit 20 includes a constant current source chip 201, a first digital potentiometer 202, and a resistor 203, the constant current source chip 202 is provided with a plurality of pins, the pins are respectively connected to the (input) power supply, the resistor 203, the excitation light source 30, and the ground, and the first digital potentiometer 202 is further respectively connected to the resistor 203 and the main control board 10.
The first digital potentiometer is used for adjusting the current output from the constant current source chip to the excitation light source by adjusting the resistance value of the resistor when receiving a resistance adjustment instruction sent by the main control board, and further adjusting the light intensity of the excitation light source, for example, the first digital potentiometer may be an MCP4162, and is connected to the main control board through an SPI interface on the MCP 4162.
The resistance is a variable resistance, the digital potentiometer can receive digital codes in various formats and establish a corresponding resistance value, the resistance adjusting instruction can include the digital codes in various formats, and the first digital potentiometer can set the resistance value of the resistance according to the resistance adjusting instruction. The input power of the constant current source chip is unchanged, the output current of the constant current source chip is inversely proportional to the resistance value, the current output to the excitation light source can be changed by changing the resistance value of the resistor, the larger the current passing through the excitation light source is, the larger the light intensity of the excitation light source is, and conversely, the smaller the current is, the smaller the light intensity of the excitation light source is, so that the adjustment of the light intensity of the excitation light source is realized.
The constant current source chip realizes constant current by reducing inductor ripple current and fixing inductor peak current, for example, the constant current source chip CN 5711. Since an inductive ripple current may be generated during resistance adjustment, the present embodiment uses a constant current source chip to ensure the stability of the circuit, and of course, the driving circuit may further include a voltage reduction chip, a photo isolator, and the like to enhance the anti-interference capability and stability of the driving circuit.
In an optional embodiment of the present invention, the main control board pre-stores resistance adjustment information, where the resistance adjustment information includes a corresponding relationship between signal strength and a resistance value, and the main control board is further configured to search for a target resistance value corresponding to the signal strength from the resistance adjustment information when the signal strength of the electrical signal is not within a preset signal strength range, and generate the resistance adjustment instruction according to the target resistance value.
In the detection process, the electrical signal of the sample to be detected is too weak or too strong to be beneficial to signal processing, so that the obtained detection result has larger error. Therefore, the memory of the main control board is pre-stored with the resistance adjustment information including the corresponding relationship between the signal strength and the resistance value, and the corresponding resistance value can be determined according to the signal strength, wherein the main control board can store the signal strength and the resistance value in the history adjustment process to obtain history data, and learn, update and the like on the history data to obtain the corresponding relationship between the signal strength and the resistance value. Through prestoring resistance adjustment information in the main control panel, the resistance in the driving circuit can be conveniently and quickly adjusted, and then the light intensity of the excitation light source can be efficiently adjusted.
In an alternative embodiment of the present invention, as shown in fig. 3, the sample detection optical path box further includes an analog switch 60 and a signal processing module 70, and the analog switch 60 is connected to the photoelectric sensor 40, the main control board 10 and the signal processing module 70 respectively.
The main control board is also used for controlling the analog switch to be closed when the signal intensity of the electric signal is within a preset signal intensity range, the photoelectric sensor is also used for sending the electric signal to the signal processing module when the analog switch is closed, and the signal processing module is used for amplifying the electric signal and then converting the electric signal into a digital signal.
The analog switch is equivalent to a signal switch, when the analog switch is closed, elements connected to two ends of the analog switch can transmit signals mutually, otherwise, the signal transmission is disconnected, and the on-off of the analog switch is controlled by the main control panel. When the signal intensity of the electric signal of the sample to be detected, which is detected by the photoelectric sensor, is within the preset signal intensity range, the signal intensity is indicated to be used for carrying out signal processing to obtain a detection result, and the error is small and the accuracy is high. Therefore, the main control board can control the analog switch to be closed so as to form a signal transmission channel between the photoelectric sensor and the signal processing module, otherwise, the analog switch is kept in an off state, the situation that the detection data with larger errors is obtained by sending the over-weak or over-strong electric signals to the signal processing module is avoided, and the calculation resource waste caused by multiple times of data processing due to the fact that multiple times of tests with larger detection errors is reduced.
In an alternative embodiment of the present invention, as shown in fig. 4, the signal processing module 70 includes a post-amplifier 701 and an AD converter 702, and the post-amplifier 701 is connected to the main control board 10 and the AD converter 702 respectively.
The main control board is also used for generating a signal amplification instruction according to the signal intensity and sending the signal amplification instruction to the rear operational amplifier, the rear operational amplifier is used for amplifying the electric signal according to the signal amplification instruction to obtain a target electric signal, and the AD converter is used for converting the target electric signal into a digital signal. The post operational amplifier comprises an operational amplifier and a digital potentiometer, wherein the digital potentiometer is used for adjusting the feedback input of the post operational amplifier, adjusting the electric signal to be within a required range and realizing controllable signal amplification.
Due to the limited detection sensitivity of the photoelectric sensor, even if the signal intensity of the electrical signal is within the preset signal intensity range, a small error may occur, for example, the actually measured electrical signal value is 3.45mV, and since the sensitivity of the photoelectric sensor can only measure 0.1mV, but cannot measure 0.01mV, the final output is 3.4mV, i.e., an error of 0.05mV is generated. Therefore, after the photoelectric sensor transmits the collected electric signal to the signal processing module, the signal processing module may first amplify the electric signal, and the specific amplification factor may be set by the main control board according to the signal intensity of the electric signal, for example, when the signal intensity of the electric signal is less than 10mV, the amplification factor is set to 100 times, and when the signal intensity of the electric signal is greater than 10mV, the amplification factor is set to 100 times. In addition, the electric signal collected by the photoelectric sensor is an analog signal, and the electric signal is also converted into a digital signal after being amplified so as to be convenient for signal processing. As shown in fig. 5, which is a schematic diagram of a graph of digital signals corresponding to electrical signals with different amplification factors, the amplification factors of the curves sequentially increase from bottom to top, and as can be seen, the larger the amplification factor is, the larger the AD value (value after AD conversion) is, the more obvious the peak shape is, and the smaller the data variance and the dispersion degree are, the easier the feature and analysis data are observed, for example, the larger the amplification factor is, the more obvious the peak value of the curve is, and the more favorable the analysis of the components of the sample to be measured is.
In an optional embodiment of the present invention, the signal processing module is further configured to send the digital signal to an upper computer, and the upper computer is configured to process the digital signal according to pre-stored detection item information to obtain detection data of the sample to be detected. The signal processing module is connected with an upper computer, the upper computer can be a computer and the like, and the upper computer displays a result value after receiving data and performing algorithm processing (including fitting, filtering, denoising and the like), so that data transmission and storage are realized.
In an optional embodiment of the present invention, the sample to be measured includes a colloidal gold sample and a fluorescent sample, and the excitation light source of the colloidal gold sample is a laser, and the excitation light source of the fluorescent sample is an LED lamp bead. The main control board outputs corresponding signals according to the test instruction so as to control the starting of different excitation light sources. For example, the test instruction includes signals such as LED _ PWM and LASER _ C, and when the LED _ PWM is set to a high level, the LED lamp bead is turned on; when the LED _ PWM is set to be at a low level, the LED lamp bead is turned off, and similarly, when the LASER _ C is set to be at a high level, the LASER is turned on; when LASER _ C is set to low level, the LASER is turned off. The upper computer can also control the adjustment of the digital potentiometer by sending instructions to the main control panel, and when the resistance value of the digital potentiometer needs to be adjusted, signals such as SCLK and CNV can be used, and the digital potentiometer can be controlled through programming.
When the sample to be detected is a fluorescence sample, the fluorescence detection device further comprises a preposed transporting and placing device, the preposed transporting and placing device is connected with the photoelectric sensor and the main control board, and the preposed transporting and placing device is used for amplifying the electric signal by preset times. Because the post-amplification operation in the current colloidal gold sample detection meets the requirement of colloidal gold signal amplification, pre-amplification is not needed. The pre-amplification is a relatively small and generally fixed value compared to the post-amplification.
To clearly explain the process of adjusting the light intensity of the sample detection light path box, the process will now be described in detail with reference to the flowchart shown in fig. 6:
s1, starting to send a test instruction to the main control board;
s2, the main control board inputs control laser driving circuit/lamp bead driving circuit to start through the terminal;
s3, lighting a laser or a LED lamp bead, and exciting the sample to be detected to emit emergent light;
s4, collecting colloidal gold signals or fluorescent signals (pre-amplifying can also be carried out), and sending the signals to a main control board;
s5, the main control board judges, when the signal intensity is out of the preset signal intensity range, the resistance in the driving circuit is adjusted, signal acquisition is carried out again, and when the signal intensity is in the preset signal intensity range, the analog switch is controlled to be turned on, and the signal is sent to the post-positioned operational amplifier;
s6, amplifying the signal by the post operational amplifier;
s7, converting the amplified signals into digital signals and sending the digital signals to an upper computer;
and S8, processing the digital signal by the upper computer to obtain detection data.
The signal intensity of the emergent light that sends after it is aroused by same excitation light source of the sample concentration that awaits measuring of different concentration is also different, through the light intensity to changing excitation light source, make excitation light source's light intensity can adapt to with the concentration of the sample that awaits measuring, the signal intensity of the signal of telecommunication that makes the sample that awaits measuring send is in predetermineeing intensity range, be difficult to when avoiding the signal of telecommunication too weak detected, detection error is great to and the signal distortion appears when the signal of telecommunication is too strong, can improve the accuracy nature of testing result.
Example two
Fig. 7 is a flowchart of a light intensity adjusting method according to a second embodiment of the present invention, which can be applied to adjusting the light intensity of an excitation light source in a sample detection light path box, as shown in fig. 7, the light intensity adjusting method includes:
and S701, driving an excitation light source to emit light when a test instruction is received so as to excite the sample to be tested to emit emergent light.
And S702, converting the optical signal of the emergent light into an electric signal.
And S703, judging whether the signal intensity of the electric signal is within a preset signal intensity range, and if not, adjusting a resistor in the driving circuit according to the signal intensity of the electric signal so as to adjust the light intensity of the excitation light source.
The light intensity adjusting method of the embodiment can be applied to the sample detection light path box provided by the first embodiment, and has the corresponding beneficial effects with the sample detection light path box. It should be noted that, as for the method embodiment, since it is basically similar to the apparatus embodiment, the description is relatively simple, and for the relevant points, reference may be made to the partial description of the apparatus embodiment.
EXAMPLE III
The third embodiment of the present invention further provides a computer-readable storage medium, on which a computer program is stored, and when the computer program is executed by a processor, the method for adjusting light intensity in any embodiment of the present invention can be implemented.
Of course, the storage medium containing the computer-executable instructions provided by the embodiments of the present invention is not limited to the method operations described above, and may also execute the light intensity adjusting method provided by any embodiment of the present invention applied to the device.
It should be noted that, as for the method and storage medium embodiments, since they are basically similar to the device embodiments, the description is relatively simple, and for the relevant points, reference may be made to the partial description of the device embodiments.
From the above description of the embodiments, it is obvious for those skilled in the art that the present invention can be implemented by software and necessary general hardware, and certainly, can also be implemented by hardware, but the former is a better embodiment in many cases. Based on such understanding, the technical solutions of the present invention may be embodied in the form of a software product, which may be stored in a computer-readable storage medium, such as a floppy disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a FLASH Memory (FLASH), a hard disk or an optical disk of a computer, and includes several instructions for enabling a computer device (which may be a personal computer, a computer device, a server, or a network device) to execute the light intensity adjusting method according to the embodiments of the present invention.
It should be noted that, in the embodiment of the sample detection optical path box, the units and modules included in the embodiment are only divided according to functional logic, but are not limited to the above division, as long as the corresponding functions can be implemented; in addition, specific names of the functional units are only for convenience of distinguishing from each other, and are not used for limiting the protection scope of the present invention.
The above-described embodiments should not be construed as limiting the scope of the invention. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions may be made in accordance with design requirements and other factors. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A sample detection light path box is characterized by comprising a main control board, a driving circuit, an excitation light source and a photoelectric sensor, wherein the main control board is respectively connected with the driving circuit and the photoelectric sensor, and the driving circuit is electrically connected with the excitation light source;
the main control board is used for sending a starting instruction to the driving circuit when receiving a test instruction;
the driving circuit is used for driving the excitation light source to emit light so as to excite the sample to be detected to emit emergent light;
the photoelectric sensor is used for converting an optical signal of the emergent light into an electric signal and sending the electric signal to the main control board;
the main control board is also used for judging whether the intensity of the electric signal is within a preset signal intensity range, and if not, generating a resistance adjusting instruction according to the signal intensity of the electric signal and sending the resistance adjusting instruction to the driving circuit;
the driving circuit is further used for adjusting the resistance in the driving circuit according to the resistance adjusting instruction so as to adjust the light intensity of the excitation light source.
2. The sample detection optical path cartridge according to claim 1, wherein a photo transistor is disposed beside the excitation light source, the photo transistor is connected to the main control board,
the phototriode is used for detecting the light intensity of the excitation light source and sending the light intensity to the main control board after the drive circuit drives the excitation light source to emit light;
the main control board is used for generating a resistance adjusting instruction according to the light intensity difference and sending the resistance adjusting instruction to the driving circuit when the light intensity of the excitation light source has the light intensity difference with the preset initial light intensity.
3. The sample detection light path cartridge according to claim 1, wherein the driving circuit comprises a constant current source chip, a first digital potentiometer and a resistor, the constant current source chip is provided with a plurality of pins, and the pins are respectively connected with the resistor, the excitation light source, a power supply and a ground; the first digital potentiometer is also connected with the resistor and the main control board respectively;
the first digital potentiometer is used for adjusting the current output to the excitation light source by the constant current source chip by adjusting the resistance value of the resistor when receiving the resistance adjusting instruction sent by the main control panel, so as to adjust the light intensity of the excitation light source.
4. The light path cassette for sample detection according to claim 1, wherein the main control board is pre-stored with resistance adjustment information, the resistance adjustment information includes a corresponding relationship between signal intensity and resistance value,
the main control board is further used for searching a target resistance value corresponding to the signal intensity from the resistance adjusting information when the signal intensity of the electric signal is not within a preset signal intensity range, and generating a resistance adjusting instruction according to the target resistance value.
5. The sample detection light path cartridge of claim 1, further comprising an analog switch and a signal processing module, wherein the analog switch is respectively connected with the photoelectric sensor, the main control board and the signal processing module,
the main control board is further used for controlling the analog switch to be closed when the signal intensity of the electric signal is within a preset signal intensity range;
the photoelectric sensor is also used for sending the electric signal to the signal processing module when the analog switch is closed;
the signal processing module is used for amplifying the electric signals and then converting the electric signals into digital signals.
6. The sample detection light path box according to claim 5, wherein the signal processing module comprises a post-loading unit and an AD converter, the post-loading unit is respectively connected with the AD converter and the main control board,
the main control board is also used for generating a signal amplification instruction according to the signal intensity and sending the signal amplification instruction to the rear transport amplifier;
the rear operational amplifier is used for amplifying the electric signal according to the signal amplification instruction to obtain a target electric signal;
the AD converter is used for converting the target electric signal into a digital signal.
7. The sample detection light path cartridge of claim 5,
the signal processing module is also used for sending the digital signal to an upper computer;
and the upper computer is used for processing the digital signals according to pre-stored detection project information to obtain detection data of the sample to be detected.
8. The sample detection optical path box according to claim 1, wherein the sample to be detected comprises a colloidal gold sample and a fluorescence sample, and when the sample to be detected is a fluorescence sample, the sample to be detected further comprises a front transport device, the front transport device is connected with the photoelectric sensor and the main control board,
the preposed operational amplifier is used for amplifying the electric signal by preset times.
9. An optical intensity adjusting method applied to the optical path box according to any one of claims 1 to 8, comprising:
when a test instruction is received, driving an excitation light source to emit light so as to excite a sample to be tested to emit emergent light;
converting the optical signal of the emergent light into an electrical signal;
and judging whether the signal intensity of the electric signal is within a preset signal intensity range, and if not, adjusting the resistance in the driving circuit according to the signal intensity of the electric signal so as to adjust the light intensity of the excitation light source.
10. A computer-readable storage medium storing computer instructions for causing a processor to implement the light intensity adjusting method of claim 9 when executed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210705111.7A CN115078312A (en) | 2022-06-21 | 2022-06-21 | Sample detection light path box, light intensity adjusting method and storage medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210705111.7A CN115078312A (en) | 2022-06-21 | 2022-06-21 | Sample detection light path box, light intensity adjusting method and storage medium |
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| Publication Number | Publication Date |
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| CN115078312A true CN115078312A (en) | 2022-09-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210705111.7A Pending CN115078312A (en) | 2022-06-21 | 2022-06-21 | Sample detection light path box, light intensity adjusting method and storage medium |
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| Country | Link |
|---|---|
| CN (1) | CN115078312A (en) |
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2022
- 2022-06-21 CN CN202210705111.7A patent/CN115078312A/en active Pending
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