CN115067318A - Dental pulp mesenchymal stem cell preservation solution and application thereof - Google Patents
Dental pulp mesenchymal stem cell preservation solution and application thereof Download PDFInfo
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- CN115067318A CN115067318A CN202210163429.7A CN202210163429A CN115067318A CN 115067318 A CN115067318 A CN 115067318A CN 202210163429 A CN202210163429 A CN 202210163429A CN 115067318 A CN115067318 A CN 115067318A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
Abstract
The invention discloses a dental pulp mesenchymal stem cell preserving fluid which is prepared from the following raw materials in parts by weight: 90-100 parts of 0.9% sodium chloride solution, 1-5 parts of penicillin, 3-6 parts of sodium hyaluronate, 5-10 parts of dipotassium hydrogen phosphate, 1.5-3 parts of 2-hydroxybenzylamine, 1-3 parts of ethyl nicotinate, 1-5 parts of polyinosinic acid, vitamin K 4 5.5 to 8.5 portions. The 2-hydroxybenzylamine, the ethyl nicotinate, the polyinosinic acid and the vitamin K in the dental pulp mesenchymal stem cell preservation solution 4 The components effectively maintain the activity of the dental pulp mesenchymal stem cells under the preservation condition of 0-6 ℃, and the like, have important significance on the short-term preservation of the dental pulp mesenchymal stem cells in vitro. The preservation solution disclosed by the invention is clear and safe in components, effectively reduces the cost for preserving the dental pulp mesenchymal stem cells in vitro, and provides convenience for short-term preservation and transportation of the dental pulp mesenchymal stem cells. The invention also provides application of the preservation solution, and the preservation solution is used for preserving dental pulp mesenchymal stem cells, is simple and convenient in preservation process and easy to operate, and provides convenience for scientific research and clinical application of cells.
Description
Technical Field
The invention relates to the field of stem cells, in particular to dental pulp mesenchymal stem cell preservation solution and application thereof.
Background
Mesenchymal stem cells (mesenchymal stem cell MSCs) are pluripotent stem cells that can form a variety of cell types. MSCs were first found in bone marrow and subsequently found in many tissues during human development and development. With the increasing maturity of mesenchymal stem cells and their related technologies, clinical research has been carried out in many fields.
At present, mesenchymal stem cells have been isolated from tissues such as bone marrow, umbilical cord, cord blood, placenta, fat, etc., and most used are bone marrow-derived mesenchymal stem cells. However, the mesenchymal stem cells derived from bone marrow have problems of difficulty in cell collection, great harm to human body, age restriction, and the like. The mesenchymal stem cells separated from the umbilical cord blood, umbilical cord and placenta tissues not only keep the biological characteristics of the mesenchymal stem cells, but also have stronger proliferation and differentiation capacities, and are harmless to the body during collection, but the collection time of tissue materials has great limitation.
The dental pulp mesenchymal stem cells have all biological characteristics of the mesenchymal stem cells, and are convenient to obtain, for example, the dental pulp mesenchymal stem cells are rich in teeth naturally shed by children of 6-11 years old and wisdom teeth required to be pulled out by adults. The dental pulp mesenchymal stem cells not only have low immunogenicity, can be used without strict pairing, can not cause strong rejection reaction, but also have the function of immunoregulation, and can not cause rejection reaction in allograft transplantation.
In the prior art, a mode of directly preserving teeth is adopted for preserving dental pulp mesenchymal stem cells, but because the number of the dental pulp mesenchymal stem cells separated from the teeth is small, a sufficient number of cells can be obtained after long-time amplification culture, and the process takes a long time. The expanded cells are directly frozen in liquid nitrogen at low temperature, and the resuspension cells are a long-term storage method when being applied, but the adoption of liquid nitrogen for freezing the dental pulp mesenchymal stem cells to be used in a short period is complicated, and the storage cost is increased. The existing low-temperature preservation solution generally has the problems of incapability of ensuring cell activity, strict requirement on preservation conditions and the like, and is not beneficial to reducing the short-term preservation cost of dental pulp mesenchymal stem cells. Therefore, there is a need to develop a preservation solution for dental pulp mesenchymal stem cells, which can realize short-term preservation of dental pulp mesenchymal stem cells in vitro and reduce the preservation cost of cells while ensuring the cell activity.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the objects of the present invention is to provide a dental pulp mesenchymal stem cell preservation solution, which realizes short-term in vitro preservation of dental pulp mesenchymal stem cells and reduces preservation cost while ensuring cell activity.
The second object of the present invention is to provide an application of the above-mentioned preservative solution.
One of the purposes of the invention is realized by adopting the following technical scheme:
the dental pulp mesenchymal stem cell preservation solution is prepared from the following raw materials in parts by weight: 90-100 parts of 0.9% sodium chloride solution, 1-5 parts of penicillin, 3-6 parts of sodium hyaluronate, 5-10 parts of dipotassium hydrogen phosphate, 1.5-3 parts of 2-hydroxybenzylamine, 1-3 parts of ethyl nicotinate, 1-5 parts of polyinosinic acid, vitamin K 4 5.5 to 8.5 portions.
Further, the dental pulp mesenchymal stem cell preservation solution is prepared from the following raw materials in parts by weight: 95 parts of sodium chloride solution with mass concentration of 0.9%, 2.5 parts of penicillin, 5 parts of sodium hyaluronate, 8 parts of dipotassium hydrogen phosphate, 2 parts of 2-hydroxybenzylamine, 2 parts of ethyl nicotinate, 3.5 parts of polyinosinic acid, and vitamin K 4 7 parts.
The second purpose of the invention is realized by adopting the following technical scheme:
the preservation solution is applied to preservation of dental pulp mesenchymal stem cells.
Further, the storage temperature was 0 to 6 ℃.
Further, the density of cells in the preservation solution was 5 × 10 6 -1×10 7 one/mL.
Further, the application of the preservation solution for dental pulp mesenchymal stem cells comprises the following steps:
(1) adding penicillin, sodium hyaluronate, dipotassium hydrogen phosphate, 2-hydroxybenzylamine, ethyl nicotinate, polyinosinic acid and vitamin K into 0.9% sodium chloride solution 4 Mixing uniformly
(2) Placing the dental pulp mesenchymal stem cell preservation solution prepared in the step (1) into an environment of 0-6 ℃ for pre-cooling, then adding the dental pulp mesenchymal stem cells to be preserved into the preservation solution, and continuing to preserve at 0-6 ℃.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a dental pulp mesenchymal stem cell preserving fluid, wherein the ingredients such as 2-hydroxybenzylamine, ethyl nicotinate and polyinosinic acid in the preserving fluid effectively maintain the activity of dental pulp mesenchymal stem cells under the preserving condition of 0-6 ℃, and the preserving fluid has important significance for short-term in-vitro preservation of the dental pulp mesenchymal stem cells. The preservation solution disclosed by the invention is clear and safe in components, effectively reduces the cost for preserving the dental pulp mesenchymal stem cells in vitro, and provides convenience for short-term preservation and transportation of the dental pulp mesenchymal stem cells.
The invention also provides application of the preservation solution, and the preservation solution is used for preserving dental pulp mesenchymal stem cells, is simple and convenient in preservation process and easy to operate, and provides convenience for scientific research and clinical application of cells.
Drawings
Fig. 1 is a graph showing proliferation curves of dental pulp mesenchymal stem cells after 48 hours of preservation and dental pulp mesenchymal stem cells without preservation in example 1 of the present invention.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict.
The sources of dental pulp mesenchymal stem cells are as follows: collecting wisdom teeth removed by 25-30 years old adult, cleaning with PBS, clenching teeth, taking out dental pulp tissue with sterile forceps, adding type I collagenase for digestion, adding complete culture medium to stop digestion, centrifuging, adding 10% FBS-containing DMEM/F12 culture medium, and resuspending at cell density of 1 × 10 4 seed/mL, in 12-well plates at 37 ℃ 5% CO 2 Culturing in the culture box, digesting with 0.25% pancreatin after the cells grow to 80% confluence, subculturing, and preserving the subcultured P3, P4 and P5 as dental pulp mesenchymal stem cells.
Example 1
The dental pulp mesenchymal stem cell preservation solution is prepared from the following raw materials in parts by weight: 95 parts of sodium chloride solution with mass concentration of 0.9%, 2.5 parts of penicillin, 5 parts of sodium hyaluronate, 8 parts of dipotassium hydrogen phosphate, 2 parts of 2-hydroxybenzylamine, 2 parts of ethyl nicotinate, 3.5 parts of polyinosinic acid, and vitamin K 4 7 parts.
The application of the preservation solution comprises the following steps:
(1) adding cyan to 0.9% sodium chloride solutionMycin, sodium hyaluronate, dipotassium hydrogen phosphate, 2-hydroxybenzylamine, ethyl nicotinate, polyinosinic acid and vitamin K 4 Mixing uniformly
(2) Placing the dental pulp mesenchymal stem cell preservation solution prepared in the step (1) into an environment of 6 ℃ for pre-cooling, then adding the P3-generation dental pulp mesenchymal stem cells into the preservation solution, wherein the density of the cells in the preservation solution is 5 multiplied by 10 6 one/mL, and continuously preserving at 6 ℃.
Example 2
The dental pulp mesenchymal stem cell preservation solution is prepared from the following raw materials in parts by weight: 90 parts of 0.9% sodium chloride solution, 1 part of penicillin, 3 parts of sodium hyaluronate, 5 parts of dipotassium hydrogen phosphate, 1.5 parts of 2-hydroxybenzylamine, 1 part of ethyl nicotinate, 1 part of polyinosinic acid, and vitamin K 4 5.5 parts.
The application of the preservation solution comprises the following steps:
(1) adding penicillin, sodium hyaluronate, dipotassium hydrogen phosphate, 2-hydroxybenzylamine, ethyl nicotinate, polyinosinic acid and vitamin K into 0.9% sodium chloride solution 4 Mixing uniformly
(2) Placing the dental pulp mesenchymal stem cell preservation solution prepared in the step (1) into an environment with the temperature of 4 ℃ for pre-cooling, then adding the P4-substituted dental pulp mesenchymal stem cells into the preservation solution, wherein the density of the cells in the preservation solution is 8 multiplied by 10 6 8/mL, and the cells were kept at 4 ℃.
Example 3
The dental pulp mesenchymal stem cell preservation solution is prepared from the following raw materials in parts by weight: 100 parts of 0.9% sodium chloride solution, 5 parts of penicillin, 6 parts of sodium hyaluronate, 10 parts of dipotassium hydrogen phosphate, 3 parts of 2-hydroxybenzylamine, 3 parts of ethyl nicotinate, 5 parts of polyinosinic acid, and vitamin K 4 8.5 parts.
The application of the preservation solution comprises the following steps:
(1) adding penicillin, sodium hyaluronate, dipotassium hydrogen phosphate, 2-hydroxybenzylamine, ethyl nicotinate, polyinosinic acid and vitamin K into 0.9% sodium chloride solution 4 Mixing uniformly
(2) Placing the dental pulp mesenchymal stem cell preservation solution prepared in the step (1) into an environment of 0 ℃ for pre-cooling, and thenAdding P5-generation dental pulp mesenchymal stem cells into a preservation solution, wherein the density of the cells in the preservation solution is 1 × 10 7 one/mL, and continuously storing at 0 ℃.
Comparative example 1
Comparative example 1 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: the same procedure as in example 1 was repeated except that 2-hydroxybenzylamine was omitted.
Comparative example 2
Comparative example 2 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: ethyl nicotinate was omitted and the procedure was as in example 1.
Comparative example 3
Comparative example 3 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: ethyl nicotinate was replaced with nicotinic acid, and the rest was the same as in example 1.
Comparative example 4
Comparative example 4 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: the ethyl nicotinate was replaced with niacinamide and the rest was the same as in example 1.
Comparative example 5
Comparative example 5 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: polyinosinic acid was omitted and the same procedure as in example 1 was repeated.
Comparative example 6
Comparative example 6 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: polyinosinic acid was replaced with inosine, and the rest was the same as in example 1.
Comparative example 7
Comparative example 7 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: mixing vitamin K 4 Replacement with vitamin K 3 Otherwise, the same as in example 1 was repeated.
Comparative example 8
Comparative example 8 provides a preservation solution for dental pulp mesenchymal stem cells, which is different from example 1 in that: mixing vitamin K 4 Replacement with vitamin K 1 Otherwise, the same as in example 1 was repeated.
The dental pulp mesenchymal stem cells stored in examples 1 to 3 and comparative examples 1 to 8 were sampled at 0h, 24h and 48h, respectively, and the cell viability was counted by trypan blue staining method, and the results are shown in table 1.
TABLE 1
| Sample (I) | 0h cell viability rate | Cell viability rate of 24h | Cell viability rate of 48h |
| Example 1 | 98.03％ | 94.79％ | 85.61％ |
| Example 2 | 98.54％ | 93.37％ | 83.15％ |
| Example 3 | 97.27％ | 93.06％ | 84.38％ |
| Comparative example 1 | 97.83％ | 84.72％ | 72.47％ |
| Comparative example 2 | 98.02％ | 82.15％ | 71.96％ |
| Comparative example 3 | 98.19％ | 79.32％ | 65.49％ |
| Comparative example 4 | 97.33％ | 85.79％ | 72.07％ |
| Comparative example 5 | 98.04％ | 80.26％ | 61.48％ |
| Comparative example 6 | 97.63％ | 86.27％ | 73.99％ |
| Comparative example 7 | 96.97％ | 89.57％ | 79.38％ |
| Comparative example 8 | 97.66％ | 85.84％ | 71.25％ |
As can be seen from table 1, the survival rates of the dental pulp mesenchymal stem cells in examples 1 to 3 were 93% or more when the cells were preserved for 24 hours, 83% or more after 48 hours of preservation, which was higher than that in comparative examples 1 to 8, in which the compositions of the preservation solutions were adjusted in comparative examples 1 to 8, respectively.
In comparative example 1, 2-hydroxybenzylamine was omitted, and the survival rate of cells decreased to various degrees with the increase of the preservation time, and after 48 hours of preservation, the survival rate of cells was 72.47%, which was lower than 85.61% in example, indicating that the addition of 2-hydroxybenzylamine contributes to the cell maintenance activity in the preservation solution.
The ethyl nicotinate is omitted in comparative example 2, the ethyl nicotinate is replaced by the nicotinic acid and the nicotinamide in comparative example 3 and comparative example 4 respectively, and the survival rate of the cells after the ethyl nicotinate is replaced by the nicotinic acid in comparative example 3 is lower than that of the cells after the ethyl nicotinate is replaced by the nicotinic acid in comparative example 2, which shows that the nicotinic acid has no obvious effect on maintaining the activity of the dental pulp mesenchymal stem cells. After replacing ethyl nicotinate with nicotinamide in comparative example 4, the cell viability was higher than in comparative example 2, but lower than in comparative example 1, indicating that the effect of adding nicotinamide was not as good as ethyl nicotinate.
In comparative example 5, which omits polyinosinic acid, and in comparative example 6, which replaces polyinosinic acid with inosine, the survival rate of the cells was decreased to a different extent, and the survival rate in comparative example 6 was higher than that in comparative example 5, but it was inferior to example 1, indicating that inosine was less effective in maintaining the activity of the dental pulp mesenchymal stem cells than polyinosinic acid.
Vitamin K was added to comparative examples 7 and 8, respectively 4 Replacement with vitamin K 3 And vitamin K 1 The survival rate of the cells was also decreased to some extent as compared with example 1, in which vitamin K was added 1 The survival rate of the cells in the preservation solution is the lowest, which indicates that the vitamin K is 4 Has better effect on maintaining the activity of the dental pulp mesenchymal stem cells.
In conclusion, the 2-hydroxybenzylamine, the ethyl nicotinate, the polyinosinic acid and the vitamin K in the preserving fluid of the invention 4 The components can effectively maintain the activity of dental pulp mesenchymal stem cells under the preservation condition of 0-6 ℃, and the dental pulp mesenchymal stem cells can be treatedHas important significance in short-term in vitro preservation. The preservation solution disclosed by the invention is clear and safe in components, effectively reduces the cost for preserving the dental pulp mesenchymal stem cells in vitro, and provides convenience for short-term preservation and transportation of the dental pulp mesenchymal stem cells.
Dental pulp mesenchymal stem cells after 48 hours of storage in example 1 were taken and seeded in 10% FBS-containing DMEM/F12 medium at a cell density of 1X 10 4 one/mL, 5% CO at 37 ℃ 2 The culture box of (1) was used for 7 days, and the number of dental pulp mesenchymal stem cells was counted every day by using the non-preserved P3-generation dental pulp mesenchymal stem cells as a control group, and a cell growth curve was drawn, with the result shown in fig. 1.
As can be seen from fig. 1, compared with the control group, the proliferation rate of the cells after 48 hours of storage provided by the invention is equivalent to that of the control group, and the number of the cells is slightly reduced compared with that of the control group, which indicates that the storage solution provided by the invention has no influence on the proliferation activity of the dental pulp mesenchymal stem cells, and the cells after storage can still be used for normal proliferation culture.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (6)
1. The preservation solution for dental pulp mesenchymal stem cells is characterized by comprising the following raw materials in parts by weight: 90-100 parts of 0.9% sodium chloride solution, 1-5 parts of penicillin, 3-6 parts of sodium hyaluronate, 5-10 parts of dipotassium hydrogen phosphate, 1.5-3 parts of 2-hydroxybenzylamine, 1-3 parts of ethyl nicotinate, 1-5 parts of polyinosinic acid, vitamin K 4 5.5 to 8.5 portions.
2. The preservation solution for dental pulp mesenchymal stem cells according to claim 1, which is prepared from the following raw materials in parts by weight: 95 parts of sodium chloride solution with mass concentration of 0.9%, 2.5 parts of penicillin, 5 parts of sodium hyaluronate, 8 parts of dipotassium hydrogen phosphate, 2 parts of 2-hydroxybenzylamine, 2 parts of ethyl nicotinate, 3.5 parts of polyinosinic acid, and vitamin K 4 7 parts.
3. The use of the preservation solution according to claim 1 in preservation of dental pulp mesenchymal stem cells.
4. The use of the preservation solution for dental pulp mesenchymal stem cells according to claim 3, wherein the preservation temperature is 0-6 ℃.
5. The use of the preservation solution for dental pulp mesenchymal stem cells according to claim 3, wherein the density of the cells in the preservation solution is 5 x 10 6 -1×10 7 one/mL.
6. The application of the preservation solution for dental pulp mesenchymal stem cells according to claim 3, which comprises the following steps:
(1) adding penicillin, sodium hyaluronate, dipotassium hydrogen phosphate, 2-hydroxybenzylamine, ethyl nicotinate, polyinosinic acid and vitamin K into 0.9% sodium chloride solution 4 Mixing uniformly
(2) Placing the dental pulp mesenchymal stem cell preservation solution prepared in the step (1) into an environment of 0-6 ℃ for pre-cooling, then adding the dental pulp mesenchymal stem cells to be preserved into the preservation solution, and continuing to preserve at 0-6 ℃.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116965403A (en) * | 2023-09-22 | 2023-10-31 | 诺莱生物医学科技有限公司 | Stem cell preservation solution and preparation method and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4055595A (en) * | 1974-12-06 | 1977-10-25 | Imperial Chemical Industries Limited | Substituted aryloxy-3,3,3-trifluoro-2-propionic acids, esters and salts thereof |
| US5919464A (en) * | 1991-05-01 | 1999-07-06 | University Of New Mexico | Treatment of aberrant cellular states with biomodulators |
| US20040170615A1 (en) * | 2002-12-11 | 2004-09-02 | Chia Soo | Methods and compositions using compounds from fetal cells and tissues to improve condition of skin |
| CN103599071A (en) * | 2013-11-08 | 2014-02-26 | 杭州美亚药业有限公司 | Preparation method of polyinosinic-polycytidylic acid dry powder |
| WO2014112675A1 (en) * | 2013-01-21 | 2014-07-24 | 경북대학교병원 | Natural cryopreservative agent for bone marrow-derived stem cells and cryopreservation method for bone marrow-derived stem cells using same |
| CN104027349A (en) * | 2014-06-26 | 2014-09-10 | 南通大学 | Application of poloxamer188 in preparation of medicines for preventing and/or treating Parkinsonism |
| CN104602521A (en) * | 2012-09-03 | 2015-05-06 | 帝斯曼知识产权资产管理有限公司 | Novel compositions comprising p-hydroxybenzylamine |
| CN107912421A (en) * | 2017-11-20 | 2018-04-17 | 温州医科大学附属第医院 | A kind of stem cell preserving fluid for Bone Marrow Mesenchymal Stem Cells Transplantation |
| US10166248B1 (en) * | 2015-12-21 | 2019-01-01 | Vanderbilt University | Methods of preventing platelet activation |
| CN112167246A (en) * | 2020-10-31 | 2021-01-05 | 郑州贝贝生物科技有限公司 | Dental pulp mesenchymal stem cell cryopreservation liquid and cryopreservation method |
| CN113812401A (en) * | 2021-11-17 | 2021-12-21 | 胡平原 | Transportation preservation liquid and preservation method for umbilical cord mesenchymal stem cells |
-
2022
- 2022-02-22 CN CN202210163429.7A patent/CN115067318B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4055595A (en) * | 1974-12-06 | 1977-10-25 | Imperial Chemical Industries Limited | Substituted aryloxy-3,3,3-trifluoro-2-propionic acids, esters and salts thereof |
| US5919464A (en) * | 1991-05-01 | 1999-07-06 | University Of New Mexico | Treatment of aberrant cellular states with biomodulators |
| US20040170615A1 (en) * | 2002-12-11 | 2004-09-02 | Chia Soo | Methods and compositions using compounds from fetal cells and tissues to improve condition of skin |
| CN102166166A (en) * | 2002-12-11 | 2011-08-31 | 苏加珞 | Method and application using compounds from fetal cells and tissues to improve skin condition |
| CN104602521A (en) * | 2012-09-03 | 2015-05-06 | 帝斯曼知识产权资产管理有限公司 | Novel compositions comprising p-hydroxybenzylamine |
| WO2014112675A1 (en) * | 2013-01-21 | 2014-07-24 | 경북대학교병원 | Natural cryopreservative agent for bone marrow-derived stem cells and cryopreservation method for bone marrow-derived stem cells using same |
| CN103599071A (en) * | 2013-11-08 | 2014-02-26 | 杭州美亚药业有限公司 | Preparation method of polyinosinic-polycytidylic acid dry powder |
| CN104027349A (en) * | 2014-06-26 | 2014-09-10 | 南通大学 | Application of poloxamer188 in preparation of medicines for preventing and/or treating Parkinsonism |
| US10166248B1 (en) * | 2015-12-21 | 2019-01-01 | Vanderbilt University | Methods of preventing platelet activation |
| CN107912421A (en) * | 2017-11-20 | 2018-04-17 | 温州医科大学附属第医院 | A kind of stem cell preserving fluid for Bone Marrow Mesenchymal Stem Cells Transplantation |
| CN112167246A (en) * | 2020-10-31 | 2021-01-05 | 郑州贝贝生物科技有限公司 | Dental pulp mesenchymal stem cell cryopreservation liquid and cryopreservation method |
| CN113812401A (en) * | 2021-11-17 | 2021-12-21 | 胡平原 | Transportation preservation liquid and preservation method for umbilical cord mesenchymal stem cells |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116965403A (en) * | 2023-09-22 | 2023-10-31 | 诺莱生物医学科技有限公司 | Stem cell preservation solution and preparation method and application thereof |
| CN116965403B (en) * | 2023-09-22 | 2023-12-15 | 诺莱生物医学科技有限公司 | Stem cell preservation solution and preparation method and application thereof |
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