CN115044467A - Production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase - Google Patents
Production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase Download PDFInfo
- Publication number
- CN115044467A CN115044467A CN202210800092.6A CN202210800092A CN115044467A CN 115044467 A CN115044467 A CN 115044467A CN 202210800092 A CN202210800092 A CN 202210800092A CN 115044467 A CN115044467 A CN 115044467A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- activity
- fermentation production
- specificity
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Medicinal Chemistry (AREA)
- Computer Hardware Design (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of fructosyltransferase production, and discloses a production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase. The invention can control the temperature in the fermentation process in real time and greatly improve the efficiency of production and fermentation.
Description
Technical Field
The invention relates to the technical field of fructosyltransferase production, in particular to a production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase.
Background
Fructosyltransferase can catalyze the intramolecular transfer of aqueous solution sucrose to generate fructo-oligosaccharide, and industrially, the fructo-oligosaccharide is a key enzyme for preparing fructo-oligosaccharide by enzymatic conversion of sucrose. Whether the enzyme can be recovered and reused is the key research focus of various research organizations and enterprises, and the fructosyltransferase is immobilized on a specific carrier as the most effective way.
According to the preparation method of the ion exchange fiber immobilized fructosyltransferase disclosed by the Chinese patent No. CN201410847613.9, liquid free fructosyltransferase is immobilized on a carrier by using weak-base anion exchange fibers as the carrier, and the activity of the immobilized fructosyltransferase can be stored for a long time and can be repeatedly used for a long time. The invention comprises two technical contents, namely preparing liquid free fructosyl transferase by using biological lysozyme and immobilizing the fructosyl transferase by using ion exchange fiber; aspergillus oryzae is fermented and cultured to obtain a large amount of thallus, washing, using cellulase to dissolve the thallus, extracting free enzyme liquid, and using ion exchange fiber to immobilize fructosyltransferase, so that the activity of the immobilized enzyme is more than or equal to 180U/g, the half-life period is more than or equal to 40 times, the conversion rate of the converted sucrose is more than or equal to 90%, and the content of fructo-oligosaccharide generated by converting the sucrose is 55-60%. Compared with the prior art, the method has the advantages of simple enzyme extraction, short operation time, easy regeneration and utilization, no cross-linking agent used in the immobilization process, high product quality safety and the like.
According to Chinese patent No. CN201110249360.1, the invention relates to a primary purification method of fructosyltransferase with macroporous anion resin as a filler, which comprises the steps of loading pretreated macroporous anion resin into a chromatographic column, flushing the chromatographic column with phosphate buffer, balancing the chromatographic column, loading a crude enzyme solution containing the fructosyltransferase, eluting with phosphate buffer with twice volume, performing gradient elution with NaC l solution of 1 mol/mL prepared from phosphate buffer with twice volume, performing online monitoring with UV280nm, collecting a peak with enzyme activity, and concentrating the collected enzyme solution with enzyme activity to obtain the fructosyltransferase. According to the invention, the enzyme liquid with high enzyme activity is obtained by a method of ball milling and ultrahigh pressure homogenization, and the fructosyltransferase is primarily purified by using the macroporous anion resin D290 as a filling material to obtain the fructosyltransferase with certain purity, so that the fructosyltransferase has very important practical significance and industrial value for producing fructo-oligosaccharide by utilizing fructose with transferase.
However, in the existing intelligent production equipment for high-activity and high-specificity fructosyl transferase, some strains required by production and processing cannot be subjected to sufficient fermentation treatment in the using process, the internal temperature in the fermentation process cannot be monitored in real time, and real-time adjustment and temperature control cannot be performed according to the temperature change in the fermentation process, the production and preparation efficiency in the using process of the existing production equipment is low, and the strains in the fermentation device cannot be subjected to sufficient and uniform heating fermentation due to the partition, so that a device is urgently needed to solve the problems.
Disclosure of Invention
The invention aims to provide a production process and intelligent production equipment of high-activity and high-specificity fructosyl transferase, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a high-activity high-specificity fructosyltransferase intelligent production equipment, includes fermentation production main part, support ring, display screen, rotates snap ring and supports the base, it is in to rotate the snap ring the joint support the upper end of base.
Furthermore, six fermentation production main bodies are uniformly arranged and distributed in a circular shape.
Furthermore, the fermentation production main body is fixedly arranged at the upper end of the rotary clamping ring, the supporting ring is fixedly connected at the upper end of the fermentation production main body, and the display screen is fixedly arranged in the middle of the supporting ring.
Furthermore, the fermentation production main body comprises a protective outer frame and a fermentation production part, and the fermentation production part is slidably mounted inside the protective outer frame.
Further, the fermentation production part comprises a sliding frame, a side protection frame, an electric heater, a rotation adjusting device, a drawing handle, a first motor, a synchronous rotating belt and a synchronous rotating wheel.
Furthermore, the side protection frames are fixedly connected to two sides of the sliding frame, the electric heaters are fixedly installed in the side protection frames, and the number of the electric heaters fixedly installed in the side protection frames on each side is three.
Compared with the prior art, the invention has the following beneficial effects:
the device comprises a supporting base, a rotary clamping ring, a display screen, a rotary clamping ring, a rotary control device and a control device, wherein the rotary clamping ring is arranged on the supporting base, the rotary control device is arranged on the display screen, the rotary control device is arranged on the rotary control device, and the rotary control device is arranged on the rotary control device.
The electric heaters are arranged, the temperature sensors can detect the temperature of the corresponding positions, and therefore the controller can control the electric heaters at the specified positions to run and stop, the fermentation temperature around each culture dish placing frame can be automatically and accurately controlled, and fermentation meets requirements.
In the process of strain fermentation treatment, the second motor is started to drive the driving bevel gear to rotate, the rotating driving bevel gear can synchronously rotate in a forward direction and a reverse direction through the synchronous rotating belt and the synchronous rotating wheel, the first rotating adjusting rod and the second rotating adjusting rod can swing in a specified amplitude through the forward rotating driving bevel gear and the reverse rotating driving bevel gear, so that the supporting and mounting table swings, the third motor is started to drive the driving gear to rotate, the rotating driving gear can drive the rotating adjusting fluted disc and the culture dish placing frame to rotate, so that strains in the culture dish placing frame can swing and rotate in the fermentation process, the strains in the culture dish placing frame can uniformly receive temperature, and uneven heating caused by blocking is avoided.
The method for evaluating the production process of the high-activity high-specificity fructosyl transferase introduces a multilayer deep neural network and a brand new loss function to evaluate the production process, assists a user to analyze the current production process through a visualization technology, and adjusts the production process when an evaluation result does not meet a preset condition, so that the production efficiency is improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the main structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the main body of the present invention;
FIG. 3 is a schematic view of the structure of a fermentation production section according to the present invention;
FIG. 4 is a schematic view of the end structure of the fermentation production section of the present invention;
FIG. 5 is a schematic view of the structure of a fermentation production section of the present invention;
FIG. 6 is a schematic view of the end structure of the fermentation production section of the present invention;
FIG. 7 is a schematic view of the rotation adjustment device of the present invention;
FIG. 8 is a side view of the rotational adjustment apparatus of the present invention;
FIG. 9 is a schematic structural view of a second embodiment of a fermentation production section according to the present invention;
fig. 10 is a flow chart of the temperature control operation of the present invention.
In the figure: 1-fermentation production main body, 2-support ring, 3-display screen, 4-rotary snap ring, 5-support base, 6-protective outer frame, 7-fermentation production part, 8-sliding frame, 9-side protective frame, 10-electric heater, 11-rotary adjusting device, 12-pull handle, 13-first motor, 14-synchronous rotating belt, 15-synchronous rotating wheel, 16-first rotary adjusting rod, 17-second motor, 18-driving bevel gear, 19-adjusting bevel gear, 20-second rotary adjusting rod, 21-third motor, 22-driving gear, 23-support mounting table, 24-culture dish placing frame, 25-rotary adjusting fluted disc, 26-controller, etc, 27-temperature sensor.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the application described herein may be used. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The invention is further described below with reference to the accompanying drawings.
Example 1
Referring to fig. 1 and fig. 2, an embodiment of the present invention includes: a production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase comprise a fermentation production main body 1, a support ring 2, a display screen 3, a rotating clamp ring 4 and a support base 5, wherein the rotating clamp ring 4 is rotationally clamped at the upper end of the support base 5, six fermentation production main bodies 1 are uniformly arranged, the six fermentation production main bodies 1 are circularly distributed, the fermentation production main bodies 1 are fixedly arranged at the upper end of the rotating clamp ring 4, the support ring 2 is fixedly connected at the upper end of the fermentation production main body 1, and the display screen 3 is fixedly arranged in the middle of the support ring 2;
referring to fig. 3 and 4, the fermentation production main body 1 comprises a protective outer frame 6 and a fermentation production part 7, the fermentation production part 7 is slidably mounted inside the protective outer frame 6, six fermentation production main bodies 1 are rotatably mounted on a support base 5 through rotating snap rings 4, and the six fermentation production main bodies 1 are circularly distributed, so that the device can complete the fermentation treatment of a large number of strains at the later stage at one time, and can observe the fermentation information and data inside each fermentation production main body 1 through a display screen 3, and after the fermentation of the strains inside the fermentation production main body 1 at the designated position is finished, the fermentation production main body 1 can rotate by adjusting the rotating snap rings 4 to take out the strains which are fermented at the corresponding position for use;
referring to fig. 5 and 6, the fermentation production part 7 includes a sliding frame 8, a side protection frame 9, electric heaters 10, a rotation adjusting device 11, a drawing handle 12, a first motor 13, a synchronous rotation belt 14 and a synchronous rotation wheel 15, the side protection frame 9 is fixedly connected to two sides of the sliding frame 8, the electric heaters 10 are fixedly installed inside the side protection frame 9, the number of the electric heaters 10 fixedly installed inside each side protection frame 9 is three, the drawing handle 12 is fixedly connected to the middle of the front end face of the side protection frame 9, the electric heaters 10 are fixedly installed inside the sliding frame 8, and the sliding frame 8 can be drawn out by the drawing handle 12;
referring to fig. 6, the synchronous rotating wheels 15 are uniformly rotatably installed on the inner end surface of the sliding frame 8, two synchronous rotating wheels 15 are provided at the middle part, the synchronous rotating belts 14 are alternately rotated and wound between the synchronous rotating wheels 15, and the front end of the first motor 13 is fixedly connected to the middle part of the outer end surface of the uppermost synchronous rotating wheel 15;
referring to fig. 7 and 8, the rotation adjusting device 11 includes a first rotation adjusting lever 16, a second motor 17, a driving bevel gear 18, an adjusting bevel gear 19, a second rotation adjusting lever 20, a third motor 21, a driving gear 22, a support mounting table 23, a culture dish placing frame 24 and a rotation adjusting fluted disc 25, the number of the first rotation adjusting lever 16 and the second rotation adjusting lever 20 is three, the first rotation adjusting lever 16 and the second rotation adjusting lever 20 are distributed in mirror symmetry, the electric heater 10 is fixedly connected to the opposite end heads of the first rotation adjusting lever 16 and the second rotation adjusting lever 20, the driving bevel gear 18 is rotatably mounted on the outer side of the adjusting bevel gear 19, the driving bevel gear 18 is engaged with the adjusting bevel gear 19 connected to the end heads of the first rotation adjusting lever 16 and the second rotation adjusting lever 20, the front end of the second motor 17 is fixedly connected to the middle portion of the driving bevel gear 18, the supporting and mounting platform 23 is fixedly connected to the front ends of the first rotating and adjusting rod 16 and the second rotating and adjusting rod 20, the driving gear 22 and the rotating and adjusting fluted disc 25 are rotatably mounted on the supporting and mounting platform 23, the driving gear 22 is meshed with the rotating and adjusting fluted disc 25, the front end of the third motor 21 is fixedly connected to the middle part of the upper end of the driving gear 22, the culture dish placing frame 24 is fixedly connected to the middle part of the upper end face of the rotating and adjusting fluted disc 25 through bolts, the second motor 17 is started to drive the driving bevel gear 18 to rotate, the rotating driving bevel gear 18 can synchronously rotate forward and backward through the synchronous rotating belt 14 and the synchronous rotating wheel 15, the driving bevel gear 18 which rotates forward and backward can enable the first rotating and adjusting rod 16 and the second rotating and adjusting rod 20 to swing with a specified amplitude through the adjusting bevel gear 19, so that the supporting and mounting platform 23 swings, and the third motor 21 is started to drive the driving gear 22 to rotate, the rotating driving gear 22 can drive the rotating adjusting fluted disc 25 and the culture dish placing frame 24 to rotate, so that the strains in the culture dish placing frame 24 can swing and rotate in the fermentation process, the strains in the culture dish placing frame 24 can uniformly receive the temperature, and the strains cannot be heated unevenly due to blocking;
referring to fig. 5, the ends of the first rotation adjusting rod 16 and the second rotation adjusting rod 20, which are away from the second motor 17, are rotatably clamped inside the side protective frame 9, the electric heater 10 and the culture dish placing frame 24 are horizontally located on the same straight line, an included angle of 120 degrees is formed between the side protective frame 9 and the sliding frame 8, and the bottom end surface of the supporting base 5 is provided with anti-slip threads.
Referring to fig. 1 and 2, the bottom end of the protective outer frame 6 is fixedly connected to the upper end of the rotary snap ring 4, and the upper end of the protective outer frame 6 is fixedly connected to the bottom end of the support ring 2;
referring to fig. 1, the upper end and the bottom end of the sliding frame 8 are slidably connected to the inside of the protective outer frame 6, so that the fermentation production part 7 can be conveniently pulled out from the inside of the protective outer frame 6;
referring to fig. 7, the synchronous rotating wheel 15 is fixedly connected to the middle of the end of the driving bevel gear 18, and the synchronous rotating belt 14 and the synchronous rotating wheel 15 can drive the driving bevel gears 18 to synchronously rotate.
Referring to fig. 5, the front end of the electric heater 10 is facing the culture dish placing frame 24, so that the hot air blown by the electric heater 10 can be fully contacted with the culture dish placing frame 24 for temperature control.
Referring to FIG. 7, the radius ratio of the driving gear 22 to the rotation adjusting gear 25 is 1:3, and the driving gear 22 can drive the rotation adjusting gear 25 to rotate slowly.
In the embodiment, six fermentation production main bodies 1 are rotatably mounted on a supporting base 5 through a rotary clamping ring 4, and the six fermentation production main bodies 1 are circularly distributed, so that the device can complete fermentation treatment of a large number of strains at the later stage at one time, and can observe fermentation information and data inside each fermentation production main body 1 through a display screen 3, after the fermentation of the strains inside the fermentation production main body 1 at a specified position is finished, the fermentation production main body 1 can rotate by adjusting the rotary clamping ring 4 to take out and use the strains at the corresponding position after the fermentation is finished, a second motor 17 is started in the process of the strain fermentation treatment to drive a bevel gear 18 to rotate, the rotary drive bevel gear 18 can realize synchronous forward and reverse rotation through a synchronous rotating belt 14 and a synchronous rotating wheel 15, and the forward and reverse rotation drive bevel gear 18 can enable a first rotation adjusting rod 16 and a second rotation adjusting rod 20 to perform synchronous forward and reverse rotation through adjusting the bevel gear 19 Appointed range's swing to make support mount table 23 swing, start third motor 21 and drive gear 22 and rotate, pivoted drive gear 22 can drive and rotate regulation fluted disc 25 and culture dish and place frame 24 and rotate, thereby the culture dish is placed the inside bacterial strain of frame 24 and can be swung and rotate at the in-process of fermentation, makes the culture dish place the bacterial strain in frame 24 can even acceptance temperature, can not receive the partition and cause the uneven of heating.
Example 2
Based on embodiment 1, as shown in fig. 9 and 10, a controller 26 is fixedly installed on the inner side of the upper end of the sliding frame 8, a temperature sensor 27 is uniformly and fixedly installed on the bottom end of the inner side of the side protection frame 9, the temperature sensor 27 is located on the outer side of the culture dish placing frame 24, and the temperature sensor 27, the electric heater 10 and the display screen 3 are electrically connected with the controller 26 through wires.
In the embodiment, the electric heater 10 is arranged to detect the temperature of the corresponding position through the temperature sensor 27, so that the controller 26 can control the electric heater 10 at the specified position to operate and stop, the fermentation temperature around each culture dish placing frame 24 can be automatically and accurately controlled, the fermentation meets the requirement, and the display screen 3 can display various readings such as the temperature inside each fermentation production main body 1.
A production process of high-activity high-specificity fructosyl transferase comprises the following specific steps:
s1, firstly, rotating the fermentation production main body 1 through external force to enable the fermentation production main body 1 and the rotary clamping ring 4 to rotate at the upper end of the supporting base 5, and then sequentially putting strains required by production and processing into the culture dish placing frame 24 in the fermentation production main body 1;
s2, after the strains are sequentially placed in the culture dish placing frames 24, the temperature around the culture dish placing frames 24 at the corresponding positions can be detected in real time through the temperature sensors 27, the temperature sensors 27 detect the temperature and transmit signals to the controller 26, and the controller 26 controls the electric heaters 10 at the corresponding positions to operate or stop according to the temperature information detected in the temperature sensors 27, so that the temperature around the culture dish placing frames 24 can be automatically controlled in real time;
s3, in the process of strain fermentation treatment, the second motor 17 is started to drive the driving bevel gear 18 to rotate, the rotating driving bevel gear 18 can realize synchronous forward and reverse rotation through the synchronous rotating belt 14 and the synchronous rotating wheel 15, the forward and reverse rotating driving bevel gear 18 can enable the first rotating adjusting rod 16 and the second rotating adjusting rod 20 to swing in a specified amplitude through the adjusting bevel gear 19, so that the supporting and mounting table 23 swings, the third motor 21 is started to drive the driving gear 22 to rotate, the rotating driving gear 22 can drive the rotating adjusting fluted disc 25 and the culture dish placing frame 24 to rotate, so that strains in the culture dish placing frame 24 can swing and rotate in the process of fermentation, the strains in the culture dish placing frame 24 can uniformly receive temperature, and heating unevenness caused by barriers can be avoided;
s4, the fermentation condition in each fermentation production main body 1 can be known according to the display screen 3, the whole fermentation production part 7 can be pulled out from the inside of the protective outer frame 6 through the pull handle 12, and therefore the strains after fermentation can be conveniently taken out from the culture dish placing frame 24.
Example 3
The embodiment 3 of the invention provides a production process evaluation method of high-activity and high-specificity fructosyl transferase, which comprises the following specific contents:
s1, obtaining the gene sequence of the fructosyl transferase.
Wherein the fructosyltransferase is obtained by the production process of the previous example.
S2, inputting the gene sequence into the trained multilayer deep neural network to obtain the mutation point of the fructosyltransferase.
The multilayer deep neural network comprises a forward propagation algorithm and a backward propagation algorithm, and a transmission formula between layers in the forward propagation algorithm is as follows: a is l ＝s(W l a l-1 +b l ) Wherein a is l Is layer I neuron output, a l-1 Is layer l-1 neuron output, s (-) is the activation function, W l Is a weight matrix connecting layer l-1 and layer l neurons, b l Are bias parameters for layer I neurons.
Further, the training process of the multilayer deep neural network comprises the following steps:
splicing gene sequences representing fructosyltransferase to construct a multidimensional vector; and inputting the multi-dimensional vector serving as a training sample into the multilayer deep neural network, and performing iterative training according to the loss function to obtain the trained multilayer deep neural network. The loss function calculation formula is as follows:
where f (θ, X) represents a loss function, N represents N training samples, and h (θ, X) i ) The predicted mutation point, y, of the ith training sample i Representing the real mutation point of the ith training sample, and t representing the dimensionality of the training sample; h (θ, X) i+1 ) Represents the predicted mutation point, y, of the i +1 th training sample i+1 Representing the true mutation point of the (i + 1) th training sample.
And S3, evaluating the production process according to the mutation point to obtain an evaluation result.
Wherein the evaluation result may include an evaluation score, a mutation analysis, a process analysis, and an improvement point analysis.
Further, the evaluation score is calculated by the following formula:
wherein score represents the evaluation score, M represents M gene sequences, P j Indicates the number of mutation points, Q, of the jth gene sequence j,k The position of a mutation point of the jth gene sequence is shown, and K represents K mutation points of the jth gene sequence.
Further, the evaluation score, mutation analysis, process analysis, and improvement point analysis are displayed in the display screen 3 to assist the user in analysis and adjustment.
S4, if the evaluation result does not meet the preset condition, adjusting the production process of the fructosyl transferase.
For example, if the evaluation score is less than 30, the production process needs to be adjusted.
The method for evaluating the production process of the high-activity and high-specificity fructosyl transferase introduces a multilayer deep neural network and a brand-new loss function, evaluates the production process, assists a user to analyze the current production process through a visualization technology, and adjusts the production process when an evaluation result does not meet a preset condition, so that the production efficiency is improved.
The working principle is as follows: firstly, the fermentation production main body 1 is rotated by external force, so that the fermentation production main body 1 and the rotary clamping ring 4 rotate at the upper end of the supporting base 5, strains required for production and processing are sequentially placed in the culture dish placing frames 24 in the fermentation production main body 1, the strains are sequentially placed in the culture dish placing frames 24, the temperature around the culture dish placing frames 24 at corresponding positions can be detected in real time through the temperature sensors 27, the temperature sensors 27 detect the temperature and transmit signals to the controller 26, the controller 26 controls the electric heaters 10 at corresponding positions to operate or stop according to temperature information detected in the temperature sensors 27, so that real-time automatic temperature control can be carried out around the culture dish placing frames 24, the second motor 17 is started in the process of strain fermentation treatment to drive the conical gear 18 to rotate, and the rotary drive conical gear 18 can realize synchronous positive rotation through the synchronous rotating belt 14 and the synchronous rotating wheel 15 The driving bevel gear 18 which rotates reversely and positively can lead the first rotating adjusting rod 16 and the second rotating adjusting rod 20 to swing with appointed amplitude through adjusting the bevel gear 19, thereby leading the supporting mounting platform 23 to swing, starting the third motor 21 to drive the driving gear 22 to rotate, leading the rotating driving gear 22 to drive the rotating adjusting fluted disc 25 and the culture dish placing frame 24 to rotate, leading the strains inside the culture dish placing frame 24 to swing and rotate in the fermentation process, leading the strains in the culture dish placing frame 24 to receive uniform temperature, avoiding uneven heating caused by barriers, leading the fermentation condition inside each fermentation production main body 1 to be known according to the display screen 3, leading the whole fermentation production part 7 to be pulled out from the inside of the protective outer frame 6 through the pull handle 12, leading the strains which are fermented to be conveniently taken out from the culture dish placing frame 24, six fermentation production main parts 1 are rotatably arranged on a supporting base 5 through rotating a clamping ring 4 in the using process of the device, and the six fermentation production main parts 1 are circularly distributed, so that the device can complete fermentation treatment of a large number of strains at the later stage at one time, and can observe fermentation information and data inside each fermentation production main part 1 through a display screen 3, after the fermentation of the strains inside the fermentation production main part 1 at a specified position is finished, the fermentation production main part 1 can rotate by adjusting the rotating clamping ring 4 to take out and use the strains with the fermentation completion at the corresponding position, a second motor 17 is started in the strain fermentation treatment process to drive a driving bevel gear 18 to rotate, the rotating driving bevel gear 18 can realize synchronous forward and reverse rotation through a synchronous rotating belt 14 and a synchronous rotating wheel 15, the driving bevel gear 18 with forward and reverse rotation can enable a first rotating adjusting rod 16 and a second rotating adjusting rod 20 to rotate through adjusting a bevel gear 19 The supporting and mounting table 23 is swung by a predetermined swing amplitude, the third motor 21 is started to drive the driving gear 22 to rotate, the rotating driving gear 22 can drive the rotation adjusting fluted disc 25 and the culture dish placing frame 24 to rotate, so that the strains in the culture dish placing frame 24 can swing and rotate in the fermentation process, the strains in the culture dish placing frame 24 can uniformly receive the temperature, the strains are not heated unevenly due to the baffle, the electric heater 10 can detect the temperature of the corresponding position through the temperature sensor 27, so that the controller 26 can control the operation and stop of the electric heater 10 at a designated position, thereby automatically and precisely controlling the fermentation temperature around each culture dish placing frame 24, so that the fermentation meets the requirement, and the display screen 3 can display various indications such as the temperature inside each fermentation production main body 1. Meanwhile, a multilayer deep neural network and a brand-new loss function are introduced to evaluate the production process, a user is assisted in evaluating the current production process through a visualization technology, and when the evaluation result does not meet the preset condition, the production process is adjusted, so that the production efficiency is improved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a high activity high specificity fructosyltransferase intelligence production facility, includes fermentation production main part (1), support ring (2), display screen (3), rotates snap ring (4) and supports base (5), its characterized in that: the rotary clamping ring (4) is rotatably clamped at the upper end of the support base (5).
2. The intelligent production equipment of high-activity and high-specificity fructosyl transferase according to claim 1, wherein: six fermentation production main parts (1) are evenly arranged, and six fermentation production main parts (1) are in circular distribution.
3. The intelligent production equipment of high-activity and high-specificity fructosyl transferase according to claim 1, wherein: fermentation production main part (1) fixed mounting rotate the upper end of snap ring (4), support ring (2) fixed connection be in the upper end of fermentation production main part (1), display screen (3) fixed mounting be in the middle part of support ring (2).
4. The intelligent production equipment of high-activity and high-specificity fructosyl transferase according to claim 1, wherein: the fermentation production main body (1) comprises a protective outer frame (6) and a fermentation production part (7), wherein the fermentation production part (7) is slidably arranged inside the protective outer frame (6).
5. The intelligent production equipment of high-activity and high-specificity fructosyl transferase according to claim 1, wherein: the fermentation production part (7) comprises a sliding frame (8), a side protection frame (9), an electric heater (10), a rotation adjusting device (11), a drawing handle (12), a first motor (13), a synchronous rotating belt (14) and a synchronous rotating wheel (15).
6. The intelligent production equipment of high-activity and high-specificity fructosyl transferase according to claim 1, wherein: the side protection frame (9) is fixedly connected to the two sides of the sliding frame (8), the electric heaters (10) are fixedly installed in the side protection frame (9), and the number of the electric heaters (10) fixedly installed in the side protection frame (9) is three.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210800092.6A CN115044467B (en) | 2022-07-07 | 2022-07-07 | Production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210800092.6A CN115044467B (en) | 2022-07-07 | 2022-07-07 | Production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115044467A true CN115044467A (en) | 2022-09-13 |
| CN115044467B CN115044467B (en) | 2023-03-24 |
Family
ID=83165018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210800092.6A Active CN115044467B (en) | 2022-07-07 | 2022-07-07 | Production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115044467B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007037487A (en) * | 2005-08-04 | 2007-02-15 | Miike Iron Works Co Ltd | Fermentative bacterium-culturing facility using organic material |
| CN104450662A (en) * | 2014-12-29 | 2015-03-25 | 临沂市宏昱生物科技有限公司 | Method for preparing immobilized fructosyltransferase through ion exchange fibers |
| CN211367534U (en) * | 2019-11-23 | 2020-08-28 | 江苏友康生态科技有限公司 | Fermentation tank for edible yeast culture |
| CN112941046A (en) * | 2021-02-09 | 2021-06-11 | 珠海高新区维得力生物工程有限公司 | Production process and production equipment of high-activity and high-specificity fructosyltransferase |
| CN216445359U (en) * | 2021-12-30 | 2022-05-06 | 山佳经典(北京)科技有限公司 | Compound fungus fermentation enzyme activity detection device |
| CN114517155A (en) * | 2022-03-17 | 2022-05-20 | 中诺生物科技发展江苏有限公司 | Preparation method and device of immobilized dextranase |
-
2022
- 2022-07-07 CN CN202210800092.6A patent/CN115044467B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007037487A (en) * | 2005-08-04 | 2007-02-15 | Miike Iron Works Co Ltd | Fermentative bacterium-culturing facility using organic material |
| CN104450662A (en) * | 2014-12-29 | 2015-03-25 | 临沂市宏昱生物科技有限公司 | Method for preparing immobilized fructosyltransferase through ion exchange fibers |
| CN211367534U (en) * | 2019-11-23 | 2020-08-28 | 江苏友康生态科技有限公司 | Fermentation tank for edible yeast culture |
| CN112941046A (en) * | 2021-02-09 | 2021-06-11 | 珠海高新区维得力生物工程有限公司 | Production process and production equipment of high-activity and high-specificity fructosyltransferase |
| CN216445359U (en) * | 2021-12-30 | 2022-05-06 | 山佳经典(北京)科技有限公司 | Compound fungus fermentation enzyme activity detection device |
| CN114517155A (en) * | 2022-03-17 | 2022-05-20 | 中诺生物科技发展江苏有限公司 | Preparation method and device of immobilized dextranase |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115044467B (en) | 2023-03-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102757891B (en) | Automatic microbial inoculation instrument | |
| CN102889699B (en) | Self-adaptive torque compensation device and working method of trough-type solar collector | |
| CN115044467B (en) | Production process and intelligent production equipment of high-activity and high-specificity fructosyltransferase | |
| CN205952404U (en) | Automatic sticking film machine based on machine vision location | |
| CN206188853U (en) | Hobbing cutter cutter ring gradient hardness heat treatment device | |
| CN105035636A (en) | Single strand chain stainless steel wire belt transmission system | |
| CN208429310U (en) | A kind of gasket production rotating disc type heat sealing machine | |
| CN116467835B (en) | Beer fermentation tank monitoring system | |
| CN216350054U (en) | Full-automatic microcomputer control mechanical testing machine | |
| CN114517155B (en) | Preparation method and device of immobilized dextranase | |
| CN205684544U (en) | A kind of I-steel digital control cold bending machine of energy warpage preventing | |
| CN216560912U (en) | Test equipment capable of testing performance of multiple motors on whole electric vehicle | |
| CN201817732U (en) | On-line automatic control device for residual stress of steel cord | |
| CN208758919U (en) | Steel ball apparatus for shaping | |
| CN114264643A (en) | Method for rapidly predicting vinegar fermentation process based on single-cell Raman spectrum | |
| CN206289308U (en) | One kind removal wire surface iron scale device | |
| CN203085061U (en) | Integrated practical training device for pharmacy | |
| CN205915166U (en) | Nozzle burnishing machine | |
| CN206781063U (en) | A kind of carbon fiber composite core rod produces detecting system | |
| CN216226529U (en) | Mold heating table | |
| CN204834332U (en) | Horizontal rotation solidification equipment of dry -type air -core reactor | |
| CN218120904U (en) | Optical fiber perform crookedness check out test set | |
| CN213060827U (en) | A roll machine for cultivateing bacterial | |
| CN205556645U (en) | Flame inoculation protection device | |
| CN109444073A (en) | A kind of nutrient quality index near infrared spectrum detection modeling analysis platform |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |