CN115039886A - Preparation method and application of bifidobacterium microcapsules - Google Patents

Preparation method and application of bifidobacterium microcapsules Download PDF

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CN115039886A
CN115039886A CN202210648170.5A CN202210648170A CN115039886A CN 115039886 A CN115039886 A CN 115039886A CN 202210648170 A CN202210648170 A CN 202210648170A CN 115039886 A CN115039886 A CN 115039886A
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bifidobacterium
coating
solution
microcapsule
mass concentration
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刘良忠
朱哲
彭利娟
何莉萍
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Wuhan Longfengyuan Biotechnology Co ltd
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Wuhan Longfengyuan Biotechnology Co ltd
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract

The invention provides a preparation method and application of a bifidobacterium microcapsule. The preparation method of the bifidobacterium microcapsules takes the porous starch as the load and protective agent, and then adopts multilayer coating, so that the survival rate of the bifidobacterium can be effectively improved, the destruction of gastric acid on the bifidobacterium is avoided, and the survival rate of the bifidobacterium reaching the intestinal tract is improved.

Description

Preparation method and application of bifidobacterium microcapsules
Technical Field
The invention relates to the technical field of preparation of probiotic products, in particular to a preparation method and application of a bifidobacterium microcapsule.
Background
The intestinal tract of a normal human body contains a large number of microorganisms, and the balance of the flora plays an important role in the health of the human body. Normal flora exists in intestinal tract as a whole, mutual dependence and mutual restriction exist, dysbacteriosis in intestinal tract of organism generates a series of physiological dysfunction, and the clinical manifestations are diarrhea, infection and the like. In recent years, probiotics (probiotics) as a general term for a group of bacteria beneficial to human bodies has special efficacy for treatment and prevention of diseases such as diarrhea and infection, has become a research hotspot of microbiology, and is widely applied to the health food industry.
The bifidobacterium is one of the most popular probiotics developed and applied at present, and has the functions of treating diseases, preventing diseases and protecting health compared with other medicaments. Bifidobacteria are the main beneficial flora of the human intestinal tract. The beneficial bacteria not only have unique effects on preventing and treating intestinal dysbacteriosis and other senile diseases, but also have the effects of inhibiting the growth and invasion of pathogenic bacteria, improving digestive function, providing nutrition, reducing serum cholesterol, improving body immunity, resisting tumors and the like. The bifidobacterium is highly sensitive to environmental factors (heat, oxygen and the like), has higher requirements on storage and transportation, is not acid-resistant, and has greatly reduced viable count after passing through gastric juice and bile salt environment at the upper end of the digestive tract, thereby greatly influencing the curative effect of the product. After the bifidobacteria are orally taken, the number of the live bacteria which can live and enter intestinal tracts is small, and the product generally has the defects of poor storage property, reduction of the number of the live bacteria along with the prolonging of the storage time and the like, so that the application of the product is limited. Therefore, it has been a hot point to study the efficient embedding and use of bifidobacterium.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation method of a bifidobacterium microcapsule, which uses porous starch as a protective agent and obtains the bifidobacterium microcapsule after three-layer coating, the three-layer microcapsule is acid-resistant, the bile salt resistance is better than that of single-layer and double-layer microcapsules, and the viable count is reduced very slowly.
The technical scheme provided by the invention is as follows: a method for preparing a bifidobacterium microcapsule comprises the following steps:
(1) preparing bifidobacterium liquid: the probiotic freeze-dried powder of bifidobacterium is dissolved in sterile water or sterilized and cooled milk, and the number of bacteria is 1 multiplied by 10 8 ～10 11 CFU/ml to obtain bifidobacterium liquid;
(2) porous starch loaded with bifidobacteria: adding the bifidobacterium liquid into the porous starch according to the mass ratio of 0.5-5: 1 of the porous starch to the bifidobacterium liquid, and uniformly stirring to obtain a porous starch mixture loaded with the bifidobacterium, or separating to obtain a porous starch mixture loaded with the bifidobacterium;
(3) coating for the first time: the first coating material is a xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution with the mass concentration of 0.5% -3%, 0.05% -0.5% of sodium isoascorbate is added into the composite solution, the first coating material and the porous starch mixture loaded with the bifidobacteria prepared in the step (2) are uniformly mixed according to the mass ratio of 1: 1-6, and the mixture is subjected to freezing or cold air drying and crushing to obtain a first coating of the bifidobacteria;
(4) coating for the second time: uniformly mixing the first bifidobacterium coating with a pectin-sodium alginate composite colloidal solution with the mass concentration of 0.3-2% according to the mass ratio of 1-3: 1, performing secondary coating, freezing or cold air drying, and crushing to obtain a second bifidobacterium coating;
(5) and (3) coating for the third time: and carrying out third coating on the second coating of the bifidobacterium and the zein ethanol solution according to the mass ratio of 1-8: 1, and freezing or cold air drying to obtain the bifidobacterium microcapsule.
Further, the bifidobacterium in the step (1) is selected from one or a mixture of more of bifidobacterium adolescentis, bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis and bifidobacterium longum.
Further, the xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution in the step (3) is prepared by mixing a xanthan gum solution with the mass concentration of 0.5-1%, a trehalose solution with the mass concentration of 0.5-2%, sodium caseinate with the mass concentration of 0.1-0.5% and a sodium alginate solution with the mass concentration of 0.5-1.5% according to the proportion of 1-5:0.2-2:0.3-3: 1-5.
Further, the pectin-sodium alginate composite colloidal solution in the step (4) is prepared by mixing a pectin solution with the mass concentration of 0.5-1.5% and a sodium alginate solution with the mass concentration of 0.5% -1.5% according to the proportion of 0.3-2: 1-5.
Further, when the third coating is carried out in the step (5), adding the zein ethanol solution into the second coating of the bifidobacteria, and uniformly mixing; or spraying zein ethanol solution on the surface of the bifidobacterium secondary coating, uniformly spraying, and blow-drying with cold air to obtain the bifidobacterium microcapsule.
Furthermore, the zein ethanol solution is obtained by dissolving zein in ethanol with the volume fraction of 70-90% according to the mass concentration of 0.5-6%.
The bifidobacterium microcapsule prepared by the invention can be applied to products such as probiotic powder, granules, solid beverages, instant foods, tablets and the like.
The bifidobacterium microcapsules prepared by the invention are applied to probiotics and prebiotics and/or dietary fiber composite solid beverage/granules, wherein the dietary fiber and/or prebiotics comprise any one or more of inulin, fructo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, trehalose, resistant dextrin, resistant starch, stachyose, xylo-oligosaccharide, oat-beta-glucan, arabinogalactan, psyllium husk powder, pectin, polydextrose and konjac glucomannan.
Further, the composite solid beverage/granule is a bifidobacterium microcapsule: the prebiotics and/or the dietary fibers are compounded according to the mass ratio of 0.1-0.5: 1-5. The prebiotics and/or the dietary fibers can be one or more of the prebiotics and/or the dietary fibers which are prepared in any proportion.
The invention has the beneficial effects that:
trehalose is a safe and reliable natural saccharide. Trehalose is a non-reducing sugar composed of two glucose molecules with 1, 1-glycosidic bonds, has 3 isomers, namely trehalose (alpha ), isophyiosaccharide (beta ) and neotrehalose (alpha, beta), and has non-specific protective effects on various bioactive substances.
The sodium caseinate is used as food additive, has high safety, and has good emulsifying and thickening effects, and can be added into various foods as required. As a coating agent, the coating effect can be improved.
The invention takes the porous starch as the protective agent, adopts multilayer coating, and the three-layer embedded bifidobacterium microcapsules are more stable than a single layer and are not easy to leak, can reduce the influence of external environmental factors (heat, oxygen and the like) and the bile salt environment at the upper end of gastric juice and digestive tract, meet the requirements of normal-temperature storage and transportation, can effectively improve the survival rate of the bifidobacterium, avoid the damage of gastric acid to the bifidobacterium, and improve the survival rate of the bifidobacterium reaching the intestinal tract. The microcapsule prepared by the preparation method has uniform particle size, smooth and seamless surface, good acid resistance and enteric solubility, and fully improves the number of viable bacteria.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1
A method for preparing a bifidobacterium microcapsule comprises the following specific steps:
(1) preparing bifidobacterium liquid: dissolving Bifidobacterium bifidum and Bifidobacterium infantis lyophilized powder in sterilized and cooled cow milk at ratio of 1:1 to obtain Bifidobacterium liquid with bacterial count of 1 × 10 10 CFU/ml；
(2) Porous starch loaded with bifidobacteria: adding the bifidobacterium liquid into the porous starch according to the mass ratio of the porous starch to the bifidobacterium liquid of 2:1, and uniformly stirring to obtain a porous starch mixture loaded with the bifidobacterium;
(3) coating for the first time: uniformly mixing a first coating material xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution, adding sodium isoascorbate with the mass fraction of 0.1% into the composite solution, and grinding the mixture of the first coating material and the porous starch-loaded bifidobacterium prepared in the step (2) at a low speed by a grinder to obtain a first coating of the bifidobacterium, wherein the mass ratio of the first coating material to the mixture is 1: 2;
the xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution is prepared by mixing a xanthan gum solution with the mass concentration of 0.5%, a trehalose solution with the mass concentration of 0.5%, sodium caseinate with the mass concentration of 0.1% and a sodium alginate solution with the mass concentration of 0.5% according to the proportion of 1:1:1: 1.
(4) Coating for the second time: uniformly mixing the first bifidobacterium coating with a pectin-sodium alginate composite colloidal solution according to the mass ratio of 1:1, performing secondary coating, freeze-drying, and dispersing at a low speed by a pulverizer to obtain a second bifidobacterium coating; the pectin-sodium alginate composite colloidal solution is prepared by mixing a pectin solution with the mass concentration of 0.5% and a sodium alginate solution with the mass concentration of 0.5% according to the proportion of 1:1.
(5) And (3) coating for the third time: and (3) coating for the third time by using a zein ethanol solution, and dissolving the zein in 80% ethanol according to the mass fraction of 1% to obtain the zein ethanol solution.
And (3) coating the bifidobacterium second coating and the zein ethanol solution for the third time according to the mass ratio of 3:1, spraying the zein ethanol solution on the surface of the bifidobacterium second coating, uniformly spraying, and drying by cold air to obtain the bifidobacterium microcapsule.
Example 2
A method for preparing a bifidobacterium microcapsule comprises the following specific steps:
(1) preparing bifidobacterium liquid: the compound Bifidobacterium includes Bifidobacterium adolescentis, Bifidobacterium animalis and Bifidobacterium infantis at ratio of 1:1:2, and is dissolved in sterile water with bacteria count of 1 × 10 9 CFU/ml；
(2) Porous starch loaded with bifidobacteria: adding the bifidobacterium liquid into the porous starch according to the mass ratio of 1:1 of the porous starch to the bifidobacterium liquid, and uniformly stirring to obtain a porous starch mixture loaded with the composite bifidobacterium;
(3) coating for the first time: the first coating material is xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution, 0.5% of sodium isoascorbate is added into the composite solution, the mass ratio of the first coating material to the porous starch-loaded bifidobacterium mixture prepared in the step (2) is 1:1.5, the first coating material and the porous starch-loaded bifidobacterium mixture are uniformly mixed, and the mixture is dried by cold air and crushed at low speed to obtain a first coating of the composite bifidobacterium;
the xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution is prepared by mixing a xanthan gum solution with the mass concentration of 0.7%, a trehalose solution with the mass concentration of 1%, sodium caseinate with the mass concentration of 0.3% and a sodium alginate solution with the mass concentration of 0.9% according to the proportion of 1.5:1.5:1: 1.5.
(4) Coating for the second time: uniformly mixing the first composite bifidobacterium coating with the pectin-sodium alginate composite colloidal solution according to the mass ratio of 1.5:1, performing secondary coating, freeze-drying, and crushing at low speed to obtain a second composite bifidobacterium coating; the pectin-sodium alginate composite colloidal solution is prepared by mixing a pectin solution with the mass concentration of 0.8% and a sodium alginate solution with the mass concentration of 1.0% in a ratio of 1: 1.5.
(5) And (3) coating for the third time: and (3) coating for the third time by using a zein ethanol solution, wherein the zein is dissolved in ethanol with the volume fraction of 90% according to the mass fraction of 1.5% to obtain the zein ethanol solution.
And (3) coating the composite bifidobacterium secondary coating and the zein ethanol solution for the third time according to the mass ratio of 3:1, adding the zein ethanol solution into the bifidobacterium secondary coating, uniformly mixing, drying by cold air, and drying at low speed to obtain the composite bifidobacterium microcapsule.
Example 3
A method for preparing a bifidobacterium microcapsule comprises the following specific steps:
(1) preparing bifidobacterium liquid: mixing Bifidobacterium breve, Bifidobacterium infantis and Bifidobacterium longum at a ratio of 1:1:1.5, and dissolving in sterilized and cooled cattleObtaining a composite bifidobacterium liquid in milk, wherein the bacterial count is 1 multiplied by 10 11 CFU/ml；
(2) Porous starch loaded with bifidobacteria: adding the bifidobacterium liquid into the porous starch according to the mass ratio of 1.5:1 of the porous starch to the composite bifidobacterium liquid, and uniformly stirring to obtain a porous starch mixture loaded with the composite bifidobacterium;
(3) coating for the first time:
the first coating material is xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution, 0.25% of sodium isoascorbate is added into the composite solution, the mass ratio of the first coating material to the mixture of the porous starch loaded composite bifidobacterium prepared in the step (2) is 1:2, the first coating material and the mixture are uniformly mixed, and freeze-dried and crushed to obtain a first coating of the bifidobacterium;
the xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution is prepared by mixing a xanthan gum solution with the mass concentration of 1%, a trehalose solution with the mass concentration of 2%, sodium caseinate with the mass concentration of 0.15% and a sodium alginate solution with the mass concentration of 1.5% in a ratio of 2:1:1: 2.
(4) Coating for the second time: uniformly mixing the first bifidobacterium coating with the pectin-sodium alginate composite colloidal solution according to the mass ratio of 1.5:1, performing secondary coating, freeze-drying and crushing to obtain a second bifidobacterium coating; the pectin-sodium alginate composite colloidal solution is prepared by mixing a pectin solution with the mass concentration of 1.5% and a sodium alginate solution with the mass concentration of 0.5% according to the proportion of 1: 2.
(5) And (3) coating for the third time: and (3) coating for the third time by using a zein ethanol solution, wherein the zein is dissolved in ethanol with the volume fraction of 85% according to the mass fraction of 5% to obtain the zein ethanol solution.
And (3) coating the second composite bifidobacterium coating and the zein ethanol solution for the third time according to the mass ratio of 3:1, adding the zein ethanol solution into the second composite bifidobacterium coating, mixing uniformly, and drying by cold air to obtain the composite bifidobacterium microcapsules.
The composite bifidobacterium microcapsule is applied to solid beverage containing probiotics, and the product formula comprises 2 g of bifidobacterium microcapsule powder, 4 g of inulin, 3 g of fructo-oligosaccharide and 1 g of stachyose.
The above description is only a detailed description of specific embodiments of the present invention, and should not be taken as limiting the invention, and any modifications, equivalents, improvements, etc. made on the design concept of the present invention should be included in the protection scope of the present invention.

Claims (8)

1. A preparation method of a bifidobacterium microcapsule is characterized by comprising the following steps:
(1) preparing bifidobacterium liquid: the probiotic freeze-dried powder of bifidobacterium is dissolved in sterile water or sterilized and cooled milk, and the number of bacteria is 1 multiplied by 10 8 ～10 11 CFU/ml to obtain bifidobacterium liquid;
(2) porous starch loaded with bifidobacteria: adding the bifidobacterium liquid into the porous starch according to the mass ratio of 0.5-5: 1 of the porous starch to the bifidobacterium liquid, and uniformly stirring to obtain a porous starch mixture loaded with the bifidobacterium, or separating to obtain a porous starch mixture loaded with the bifidobacterium;
(3) coating for the first time: the first coating material is a xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution with the mass concentration of 0.5% -3%, 0.05% -0.5% of sodium isoascorbate is added into the composite solution, the first coating material and the porous starch mixture loaded with the bifidobacteria prepared in the step (2) are uniformly mixed according to the mass ratio of 1: 1-6, and the mixture is subjected to freezing or cold air drying and crushing to obtain a first coating of the bifidobacteria;
(4) coating for the second time: uniformly mixing the first bifidobacterium coating with a pectin-sodium alginate composite colloidal solution with the mass concentration of 0.3-2% according to the mass ratio of 1-3: 1, performing secondary coating, freezing or cold air drying, and crushing to obtain a second bifidobacterium coating;
(5) and (3) coating for the third time: and carrying out third coating on the second coating of the bifidobacterium and the zein ethanol solution according to the mass ratio of 1-8: 1, and freezing or cold air drying to obtain the bifidobacterium microcapsule.
2. A method of preparing a bifidobacterium microcapsule according to claim 1 wherein: the bifidobacterium in the step (1) is selected from one or a mixture of more of bifidobacterium adolescentis, bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis and bifidobacterium longum.
3. A method of preparing a bifidobacterium microcapsule according to claim 1 wherein: the xanthan gum-trehalose-sodium caseinate-sodium alginate composite solution in the step (3) is prepared by mixing a xanthan gum solution with the mass concentration of 0.5-1%, a trehalose solution with the mass concentration of 0.5-2%, sodium caseinate with the mass concentration of 0.1-0.5% and a sodium alginate solution with the mass concentration of 0.5-1.5% according to the mass ratio of 1-5:0.2-2:0.3-3: 1-5.
4. A method of preparing a bifidobacterium microcapsule according to claim 1 wherein: the pectin-sodium alginate composite colloidal solution in the step (4) is prepared by mixing a pectin solution with the mass concentration of 0.5-1.5% and a sodium alginate solution with the mass concentration of 0.5-1.5% according to the mass ratio of 0.3-2: 1-5.
5. A method of preparing a bifidobacterium microcapsule according to claim 1 wherein: when the third coating is carried out in the step (5), adding the zein ethanol solution into the second coating of the bifidobacteria, and uniformly mixing; or spraying zein ethanol solution on the surface of the bifidobacterium secondary coating, uniformly spraying, and blow-drying with cold air to obtain the bifidobacterium microcapsule.
6. A method of preparing a Bifidobacterium microcapsule according to claim 5 wherein: the zein ethanol solution is obtained by dissolving zein in ethanol with the volume fraction of 70-90% according to the mass concentration of 0.5-6%.
7. Use of a bifidobacterium microcapsule prepared according to any of claims 1 to 6 in which: the solid beverage/granule is applied to probiotics and prebiotics and/or dietary fiber composite solid beverage/granule, wherein the dietary fiber and/or prebiotics comprise one or more of inulin, fructo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, trehalose, resistant dextrin, resistant starch, stachyose, xylo-oligosaccharide, oat-beta-glucan, arabinogalactan, psyllium husk powder, pectin, polydextrose and konjac glucomannan.
8. Use of a bifidobacterium microcapsule according to claim 7 in which: the composite solid beverage/granule is a bifidobacterium microcapsule: the prebiotics and/or the dietary fibers are compounded according to the mass ratio of 0.1-0.5: 1-5.
CN202210648170.5A 2022-06-09 2022-06-09 Preparation method and application of bifidobacterium microcapsules Pending CN115039886A (en)

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CN113558246A (en) * 2021-07-21 2021-10-29 石河子大学 Symbiotic bifidobacterium composite microcapsule and preparation method thereof
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CN102960598A (en) * 2012-11-19 2013-03-13 陕西科技大学 Method for preparing three-layer embedded bifidobacterium microcapsules
CN103283975A (en) * 2013-03-27 2013-09-11 广州格拉姆生物科技有限公司 A method of using spray drying for preparing probiotics micro-capsules
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