CN115014912A

CN115014912A - Pathological staining machine

Info

Publication number: CN115014912A
Application number: CN202210578477.2A
Authority: CN
Inventors: 陶洁; 何子昕; 彭为生
Original assignee: Zhuhai Senlong Biotech Co ltd
Current assignee: Zhuhai Senlong Biotech Co ltd
Priority date: 2022-05-26
Filing date: 2022-05-26
Publication date: 2022-09-06

Abstract

The invention discloses and provides a pathological staining machine which can effectively avoid the influence of reagent exposure on staining quality and avoid potential safety hazard. The pathological staining machine comprises a machine body, a slide frame lifting device arranged in the machine body, a slide frame taking and placing device arranged on the slide frame lifting device, a plurality of closed type kits located below the slide frame taking and placing device and a kit cover opening device connected with the slide frame lifting device, wherein the slide frame lifting device controls the slide frame taking and placing device to downwards clamp a slide frame, the kit cover opening device opens a box cover of the closed type kit, when the slide frame lifting device upwards rises, the kit cover opening device is separated from contact with the closed type kits, and the closed type kit is reset to be in a closed type structure. The invention can be widely applied to the field of medical appliances.

Description

Pathological staining machine

Technical Field

The invention relates to a pathological staining machine.

Background

With the development of pathological techniques, a pathological staining machine is commonly used in clinical laboratories or pathology departments of some hospitals to stain blood pictures, bone marrow slices, and the like, and the staining technique is used to stain tissue cell slices to observe and judge whether or not the tissue cells of a subject have lesions or judge the lesion degree, so that the pathological staining machine is a widely applied technique in current pathological diagnosis. The common dyeing vat type automatic slide dyeing machine on the market is also a dyeing vat type automatic slide dyeing machine, a plurality of dyeing vats filled with reagents are adopted, the slide dyeing machine uses a mechanical arm to sequentially place slides into different dyeing vats and stay for a certain time, so that colored sample slides are obtained, the reagents in the dyeing vats are bound to be exposed in the air for a long time, such as xylene reagents which are toxic and volatile substances, flammable and explosive, and exposed in the air, the dyeing quality is influenced, potential safety hazards exist, moisture evaporation of the reagents is increased due to the room temperature effect and the dehumidifying function of an air conditioner, for hospitals with small scale and sample amount, the reagent replacement interval is prolonged when the machine is used, the dyeing quality is reduced, and the cost is increased if the machine is not used up for several times.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a pathological staining machine which can effectively avoid the influence of reagent exposure on staining quality and avoid potential safety hazards.

The technical scheme adopted by the invention is as follows: the pathological staining machine comprises a machine body, a slide frame lifting device arranged in the machine body, a slide frame taking and placing device arranged on the slide frame lifting device, a plurality of closed reagent boxes positioned below the slide frame taking and placing device and a reagent box uncovering device connected with the slide frame lifting device, the closed reagent kit comprises a reagent kit body, a reagent kit cover rotationally connected with the reagent kit body through a second shaft pin, and a second elastic pressure spring arranged on the second shaft pin, the slide rack lifting device controls the slide rack taking and placing device to place the slide rack downwards, the reagent box cover opening device opens the reagent box cover, when the slide frame lifting device ascends, the reagent box cover opening device is separated from contact with the reagent box cover, and the reagent box cover is reset to cover the reagent box body under the action of the second elastic compression spring.

Preferably, the slide frame lifting device comprises a lifting motor base, a lifting motor arranged on the lifting motor base, a driving transmission wheel arranged on a rotating shaft of the lifting motor, and a driven transmission wheel arranged on the lifting motor base and connected with the driving transmission wheel through a transmission belt, the slide frame taking and placing device is connected with one side of the transmission belt, the reagent box cover opening device is connected with the other side of the transmission belt, when the slide frame taking and placing device is placed to descend, the reagent box cover opening device opens the closed reagent box, and when the slide frame taking and placing device ascends, the closed reagent box recovers the closed state.

Preferably, the slide frame taking and placing device comprises a slide frame taking and placing seat, a slide frame clamping piece, a third elastic pressure spring, an elastic positioning pin and an attraction magnet, wherein the slide frame taking and placing seat is arranged on the lifting motor seat, is in sliding fit with the lifting motor seat and is connected with the transmission belt, the slide frame clamping piece is rotatably connected with the slide frame taking and placing seat through a third shaft pin, the third elastic pressure spring is arranged on the third shaft pin, the elastic positioning pin is vertically arranged on two sides of the slide frame taking and placing seat and is matched with the slide frame, the attraction magnet is arranged on the slide frame taking and placing seat and is opposite to the top of the slide frame clamping piece, and a buckling part on the slide frame clamping piece is clamped on the slide frame and is fixed to the slide frame under the auxiliary action of the elastic positioning pin.

Preferably, the reagent kit cover opening device comprises a transmission push block arranged on the transmission belt, a connecting rod arranged on the lifting motor base and in sliding fit with the lifting motor base, a cover opening slide block arranged on the lifting motor base in a sliding way through a guide rod and connected with the connecting rod, a guide rod fixing block arranged on the lifting motor base and fixedly connected with the bottom of the guide rod, a reset spring sleeved on the guide rod and positioned between the cover opening slide block and the guide rod fixing block, and a cover opening head arranged on the cover opening slide block, when the cover is opened, the driving belt drives the driving push block to move upwards, the cover opening slide block is jacked upwards after the return spring recovers the elasticity, the cover of the kit cover is gradually pushed open by the cover opening head, and the glass slide on the slide frame smoothly enters the closed kit for dyeing.

Preferably, the pathology slide dyeing machine further comprises a slide feeding area, a slide baking area and a slide dyeing area which are located at the bottom in the machine body, at least one slide frame bearing seat located at the slide feeding area, at least one direct-heating constant-temperature slide baking seat located at the slide baking area, and a mechanical arm arranged above the machine body and used for moving the slide frame from different areas to take and place, wherein the closed type kit is arranged at the slide dyeing area.

Preferably, the slide frame bearing seat is of a drawer type structure in sliding fit with the machine body, the slide frame bearing seat comprises a bearing seat body, a slide frame accommodating groove formed in the top of the bearing seat body, a sliding groove formed in the bottom of the bearing seat body, and a handle groove formed in one side, close to the outer side, of the bearing seat body, the sliding groove is in sliding fit with the guide portion in the bottom of the machine body, and the handle groove is located outside the machine body.

Preferably, the mechanical arm comprises a transverse transmission device and the slide rack lifting device, and the transverse transmission device drives the slide rack lifting device to move transversely.

Preferably, the transverse transmission device comprises a transverse transmission frame, a transverse driving wheel arranged on the transverse transmission frame, a transverse transmission belt connected with the transverse driving wheel, and a transverse driven wheel arranged on the transverse transmission frame and connected with the transverse transmission belt, and the transverse transmission belt is connected with the lifting motor base.

Preferably, the closed reagent kit further comprises a reagent cavity arranged in the reagent kit body, a reagent sample adding box cover arranged on the reagent kit body, and a liquid level sensor arranged in the reagent cavity.

Optimized, the reagent chamber divide into reagent application of sample district, reagent reaction zone and the reagent response district that communicates each other, first pressure head on the second elastic pressure spring is pressed the surface of kit box lid, second pressure head on the second elastic pressure spring then with the inboard of kit box body contacts, reagent application of sample lid pass through the pivot with kit box body normal running fit be provided with on the kit box body with the application of sample mouth of reagent application of sample district intercommunication, the liquid level inductor sets up in the reagent response district.

The invention has the beneficial effects that: because the pathological staining machine comprises a machine body, a slide frame lifting device arranged in the machine body, a slide frame taking and placing device arranged on the slide frame lifting device, a plurality of closed reagent kits positioned below the slide frame taking and placing device and a reagent kit cover opening device connected with the slide frame lifting device, wherein each closed reagent kit comprises a reagent kit box body, a reagent kit cover rotationally connected with the reagent kit box body through a second shaft pin and a second elastic pressure spring arranged on the second shaft pin, the slide frame lifting device controls the slide frame taking and placing device to downwards clamp a slide frame, the reagent kit cover opening device opens the reagent kit cover, when the slide frame lifting device ascends, the reagent kit cover opening device is separated from contact with the reagent kit cover, and the reagent kit cover resets to cover the reagent kit box body under the action of the second elastic pressure spring, through the structure, in a conventional state, a reagent is sealed in the closed reagent box, so that the reagent is not exposed for a long time and does not cause volatilization of the reagent, and when reagent dyeing is required, the reagent box cover is jacked open by the reagent box cover opening device, so that the slide frame can enter the closed reagent box to perform dyeing work in a short time, the dyeing quality can be effectively prevented from being influenced and potential safety hazards can be avoided due to reagent exposure.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic perspective view of the interior of the present invention;

FIG. 3 is a schematic front perspective view of the slide rack lifting device of the present invention;

FIG. 4 is a schematic view of the front exploded view of the slide rack lifting device of the present invention;

FIG. 5 is a schematic view of the back side of the slide rack lift apparatus of the present invention;

FIG. 6 is a schematic perspective view of the slide rack pick-and-place apparatus of the present invention;

FIG. 7 is an exploded view of the slide rack pick and place apparatus of the present invention;

FIG. 8 is a schematic view of an exploded configuration of the slide rack of the present invention;

FIG. 9 is a schematic perspective view of a lid opening device for reagent kit according to the present invention;

FIG. 10 is a schematic view showing an explosive structure of the lid opening device of the reagent vessel of the present invention;

FIG. 11 is a schematic view showing an exploded structure of a closed type reagent kit of the present invention;

FIG. 12 is a schematic sectional structure view of a closed-type reagent cartridge of the present invention;

FIG. 13 is a schematic perspective view of a slide mount carrier of the present invention;

FIG. 14 is an exploded view of a direct-heating thermostatic baking sheet holder according to the present invention.

Detailed Description

The technical solution of the present application will be described in detail with reference to the specific embodiments.

As shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7, fig. 8, fig. 9, fig. 10, fig. 11, fig. 12, fig. 13, fig. 14, the present invention claims a fully automated pathology staining machine, which comprises a machine body 101, a slide feeding zone 102, a baking zone 103 and a staining zone 104 located at the bottom inside the machine body 101, at least one slide rack carrier 1 located at the slide feeding zone 102, at least one direct-heating thermostatic baking rack 2 located at the baking zone 103, a plurality of enclosed reagent boxes 3 located at the staining zone 104, a robot arm 105 disposed above the machine body 101 for moving the slide racks 4 from different areas to and from, a slide rack pick-and-place device 106 disposed on the robot arm 105, and a reagent box cover-open device 107 connected to the robot arm 105, the slide rack carrier 1 being a drawer type structure in sliding fit with the machine body 101, the slide rack 4 can slide out of the slide inlet area 102 to be supported by the outside and then be retracted into the slide inlet area, the mechanical arm 105 is used for placing the slide rack 4 into the baking area 103 or the slide inlet area 104 or the cleaning area to work according to different requirements, the reagent is sealed in the closed reagent box 3 under the normal state, the reagent is not exposed for a long time and can not volatilize, and when the reagent is required to be dyed, the reagent box cover 33 is pushed open through the reagent box cover opening device 107, so that the slide rack 4 can enter the closed reagent box 3 to be dyed for a short time, the invention can effectively avoid the reagent exposure from influencing the dyeing quality and avoid the potential safety hazard, most importantly, under the control of one set of the slide rack lifting device 109, meanwhile, the cover opening and the placement of the slide rack 4 can be completed, so that the parts are reduced, the structure is simple, the volume and the occupied space of the product are reduced, and the operation steps are saved to improve the efficiency.

The slide holder carrying seat 1 is mainly used for carrying the slide holder 4, the slide holder carrying seat 1 comprises a carrying seat body 11, a slide holder accommodating groove 12 arranged at the top of the carrying seat body 11, a sliding groove 13 arranged at the bottom of the carrying seat body 11 and a handle groove 14 arranged at one side of the carrying seat body 11 close to the outside, the sliding groove 13 is in sliding fit with a guide part at the top of the machine body 101, the handle groove 14 is positioned at the outside of the machine body 101 so that an operator can pull out the slide holder carrying seat 1, the slide holder accommodating groove 12 is used for accommodating the slide holder 4, a plurality of 4 slides can be clamped on the slide holder 4, the slide holder 4 comprises a slide holder body 41, a slide holding piece 43 rotatably connected with the slide holder body 41 through a first shaft pin 42, and a first elastic pressure spring 44 arranged on the first shaft pin 42, the top surface of the slide frame body 41 is provided with slide frame positioning holes 45. The direct-heating constant-temperature type baking sheet seat 2 comprises a baking sheet aluminum seat body 21, a glass sheet baking sheet cavity 22 arranged on the baking sheet aluminum seat body 21 and a baking sheet heating body 23 arranged in the glass sheet baking sheet cavity 22, wherein the heating of the baking sheet heating body 23 can enable the baking sheet aluminum seat body 21 to be directly heated and to be constant in temperature.

The closed reagent kit 3 comprises a reagent kit body 31, a reagent chamber 32 arranged in the reagent kit body 31, a reagent kit cover 33 and a reagent sample adding cover 35 which are arranged on the reagent kit body 31, and a liquid level sensor 34 arranged in the reagent chamber 32, wherein the reagent chamber 32 is divided into a reagent sample adding area 321, a reagent reaction area 322 and a reagent sensing area 323 which are mutually communicated, the reagent kit cover 33 is rotatably connected with the reagent kit body 31 through a second shaft pin 37, a second elastic pressure spring 36 is sleeved on the second shaft pin 37, a first pressure head 361 on the second elastic pressure spring 36 is pressed on the surface of the reagent kit cover 33, a second pressure head 362 on the second elastic pressure spring 36 is contacted with the inner side of the reagent kit body 31, the reagent chamber 32 is in a sealed state under a normal state, and the actions of opening and closing the cover can be completed by matching with the mechanical arm 105, and reagent application of sample lid 35 through the pivot pin with reagent box body 31 normal state, reagent application of sample lid 35 seals application of sample mouth 324 that communicates with reagent application of sample district 321 is provided with on the reagent box body 31, and under the conventional state, reagent application of sample lid 35 seals application of sample mouth 324, then when needing, through opening reagent application of sample lid 35 back through application of sample mouth 324 add reagent, liquid level inductor 34 then sets up in reagent induction zone 323, go to send information through the liquid level that senses in real time and remind whether need to add reagent.

As the key point of the present invention, the mechanical arm 5 comprises a transverse transmission device 108 and a slide rack lifting device 109, the slide rack lifting device 109 comprises a lifting motor base 5, a lifting motor 6 arranged on the lifting motor base 5, a driving transmission wheel 7 arranged on a rotating shaft of the lifting motor 6, and a driven transmission wheel 9 arranged on the lifting motor base 5 and connected with the driving transmission wheel 7 through a transmission belt 8, the slide rack taking and placing device 106 is connected with one side of the transmission belt 8, the reagent box opening device 107 is connected with the other side of the transmission belt 8, so that when the slide rack taking and placing device 106 takes and lowers the slide rack 4, the reagent box opening device 107 opens the closed reagent box 3, and when the slide rack taking and placing device 106 takes and raises the slide rack 4, the reagent box uncovering device 107 is disabled, and the closed reagent box 3 is restored to a closed state. Specifically, the slide rack taking and placing device 106 includes a slide rack taking and placing base 15 disposed on the lifting motor base 5 and slidably engaged with the lifting motor base 5 and connected with the transmission belt 8, slide rack clamping pieces 17 rotatably connected with the slide rack taking and placing base 15 through third shaft pins 16, third elastic compression springs 19 disposed on the third shaft pins 16, elastic positioning pins 18 vertically disposed on both sides of the slide rack taking and placing base 15 and engaged with the slide racks 4, and attracting magnets 20 disposed on the slide rack taking and placing base 15 at positions opposite to the tops of the slide rack clamping pieces 17, wherein the slide rack taking and placing base 15 moves downward under the driving of the transmission belt 8, the slide rack clamping pieces 17 rotate under the action of the attracting magnets 20 after overcoming the elastic action of the third elastic compression springs 19, so that a sufficient space is opened between the slide rack clamping pieces 17 and the slide rack taking and placing base 15, after the attraction magnet 20 is out of order, the third elastic compression spring 19 recovers the elastic force to return the slide holder holding piece 17, then the buckling part 171 on the slide holder holding piece 17 is buckled on the slide holder 4 and is fixed on the slide holder 4 under the auxiliary action of the elastic positioning pin 18, and at this time, the bottom of the elastic positioning pin 18 is positioned in the slide holder positioning hole 45. The reagent box cover opening device 107 comprises a transmission push block 24 arranged on the transmission belt 8, a connecting rod 25 arranged on the lifting motor base 5 and in sliding fit with the lifting motor base 5, a cover opening slide block 27 arranged on the lifting motor base 5 through a guide rod 26 and in sliding fit with the connecting rod 25, a guide rod fixing block 28 arranged on the lifting motor base 5 and fixedly connected with the bottom of the guide rod 26, a return spring 29 sleeved on the guide rod 26 and positioned between the cover opening slide block 27 and the guide rod fixing block 28, and a cover opening head 30 arranged on the cover opening slide block 27, and it should be specifically noted that the cover opening head 30 is positioned below the reagent box cover 33, the reagent box cover 33 can exceed the reagent box body 31 by a little distance so as to smoothly enable the cover opening head 30 to move transversely without interference, under the conventional state, the closed reagent kit 3 is in the closed state, the connecting rod 25 is in under the effect of the transmission push block 24, so that the cover opening slide block 27 is located at the lower end of the guide rod 26, at the moment, the guide rod 26 presses the return spring 29, the cover opening head 30 can move transversely, when the cover is required to be opened, the transmission belt 8 drives the transmission push block 24 to move upwards, at the moment, the return spring 29 can slowly recover the elasticity, so that the cover opening slide block 27 is pushed upwards, at the moment, the cover opening head 30 gradually pushes the reagent kit cover 33, so that the slide glass on the slide rack 4 conveniently and smoothly enters the reagent kit for dyeing, and on the contrary, after the return spring 29 is squeezed by the cover opening slide block 27, the reagent kit cover 33 recovers the sealing under the effect of the second elastic pressure spring 36. The transverse transmission device 108 mainly functions to transversely move the slide rack lifting device 109, and specifically includes a transverse transmission frame 37, a transverse driving wheel 38 disposed on the transverse transmission frame 37, a transverse transmission belt 39 connected to the transverse driving wheel 38, and a transverse driven wheel 40 disposed on the transverse transmission frame 37 and connected to the transverse transmission belt 39, wherein the transverse transmission belt 39 is connected to the lifting motor base 5.

The invention can be widely applied to the field of medical appliances.

Claims

1. A pathological staining machine is characterized in that: the pathological staining machine comprises a machine body (101), a slide frame lifting device (109) arranged in the machine body (101), a slide frame taking and placing device (106) arranged on the slide frame lifting device (109), a plurality of closed reagent kits (3) positioned below the slide frame taking and placing device (106), and a reagent kit cover opening device (107) connected with the slide frame lifting device (109), wherein the closed reagent kits (3) comprise a reagent kit box body (31), a reagent kit box cover (33) rotationally connected with the reagent kit box body (31) through a second shaft pin (37), and a second elastic pressure spring (36) arranged on the second shaft pin (37), the slide frame lifting device (109) controls the slide frame taking and placing device (106) to downwards place a slide frame (4), and the reagent kit cover opening device (107) opens the reagent kit box cover (33), when the slide frame lifting device (109) ascends, the reagent box cover opening device (107) is separated from contact with the reagent box cover (33), and the reagent box cover (33) resets under the action of the second elastic compression spring (36) to cover the reagent box body (31).

2. The pathology staining machine of claim 1, wherein: the slide rack lifting device (109) comprises a lifting motor base (5), a lifting motor (6) arranged on the lifting motor base (5), a driving transmission wheel (7) arranged on a rotating shaft of the lifting motor (6), and a driven transmission wheel (9) arranged on the lifting motor base (5) and connected with the driving transmission wheel (7) through a transmission belt (8), wherein the slide rack taking and placing device (106) is connected with one side of the transmission belt (8), the reagent box uncovering device (107) is connected with the other side of the transmission belt (8), when the slide rack taking and placing device (106) takes and descends, the reagent box uncovering device (107) opens the closed reagent box (3), and when the slide rack taking and placing device (106) ascends, the closed reagent box (3) restores to a closed state.

3. The pathology staining machine of claim 2, wherein: the slide rack taking and placing device (106) comprises a slide rack taking and placing seat (15) which is arranged on the lifting motor seat (5) and is in sliding fit with the lifting motor seat (5) and is connected with the transmission belt (8), a slide rack clamping sheet (17) which is rotationally connected with the slide rack taking and placing seat (15) through a third shaft pin (16), a third elastic pressure spring (19) which is arranged on the third shaft pin (16), elastic positioning pins (18) which are vertically arranged on two sides of the slide rack taking and placing seat (15) and are matched with the slide rack (4), and an attraction magnet (20) which is arranged on the slide rack taking and placing seat (15) and is opposite to the top of the slide rack clamping sheet (17), the buckling part (171) on the slide holder clamping piece (17) is clamped on the slide holder (4), and the glass slide frame (4) is fixed under the auxiliary action of the elastic positioning pin (18).

4. The pathology staining machine of claim 2, wherein: the reagent box cover opening device (107) comprises a transmission push block (24) arranged on the transmission belt (8), a connecting rod (25) arranged on the lifting motor base (5) and in sliding fit with the lifting motor base (5), a cover opening slide block (27) arranged on the lifting motor base (5) and connected with the connecting rod (25) through a guide rod (26), a guide rod fixing block (28) arranged on the lifting motor base (5) and fixedly connected with the bottom of the guide rod (26), a return spring (29) sleeved on the guide rod (26) and positioned between the cover opening slide block (27) and the guide rod fixing block (28) and a cover opening head (30) arranged on the cover opening slide block (27), wherein when the cover is opened, the transmission belt (8) drives the transmission push block (24) to move upwards, and the cover opening slide block (27) is pushed upwards after the return spring (29) recovers elasticity, the cover opening head (30) gradually pushes the kit cover (33) open, and the glass slide on the glass slide frame (4) smoothly enters the closed kit (3) for dyeing.

5. The pathology staining machine of any of claims 1-4, wherein: pathological staining machine is still including being located organism (101) bottom advance piece district (102), roast piece district (103) and stain piece district (104), be located advance at least one slide frame of piece district (102) department and bear seat (1), be located roast piece of district (103) department at least one directly hot thermostatic type roast piece seat (2), set up and be in organism (101) top is used for with slide frame (4) are from different regional removal arm (105) of getting and put, closed kit (3) set up stain piece district (104) department.

6. The pathology staining machine of claim 5, wherein: the slide frame bearing seat (1) is of a drawer type structure in sliding fit with the machine body (101), the slide frame bearing seat (1) comprises a bearing seat body (11), a slide frame accommodating groove (12) formed in the top of the bearing seat body (11), a sliding groove (13) formed in the bottom of the bearing seat body (11) and a handle groove (14) formed in one side, close to the outer side, of the bearing seat body (11), the sliding groove (13) is in sliding fit with a guide portion in the bottom of the machine body (101), and the handle groove (14) is located outside the machine body (101).

7. The pathological staining machine of claim 5, wherein: the mechanical arm (105) comprises a transverse transmission device (108) and a slide rack lifting device (109), and the transverse transmission device (108) drives the slide rack lifting device (109) to move transversely.

8. The pathology staining machine of claim 7, wherein: the transverse transmission device (108) comprises a transverse transmission frame (37), a transverse driving wheel (38) arranged on the transverse transmission frame (37), a transverse transmission belt (39) connected with the transverse driving wheel (38) and a transverse driven wheel (40) arranged on the transverse transmission frame (37) and connected with the transverse transmission belt (39), and the transverse transmission belt (39) is connected with the lifting motor base (5).

9. The pathology staining machine of any of claims 1-4, wherein: the closed reagent kit (3) further comprises a reagent cavity (32) arranged in the reagent kit body (31), a reagent sample adding box cover (35) arranged on the reagent kit body (31) and a liquid level sensor (34) arranged in the reagent cavity (32).

10. The pathology staining machine of claim 9, wherein: reagent chamber (32) divide into reagent that communicates each other and adds appearance district (321), reagent reaction district (322) and reagent induction zone (323), first pressure head (361) on second elasticity pressure spring (36) are pressed the surface of kit lid (33), second pressure head (362) on second elasticity pressure spring (36) then with the inboard of kit box body (31) contacts, reagent application of sample lid (35) through the pivot with kit box body (31) normal running fit be provided with on kit box body (31) with sample loading mouth (324) that reagent adds appearance district (321) intercommunication, liquid level inductor (34) set up in reagent induction zone (323).