CN115011645B - Application of picolinic acid in preparation of biopesticide for preventing and treating xanthomonas diseases - Google Patents

Application of picolinic acid in preparation of biopesticide for preventing and treating xanthomonas diseases Download PDF

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CN115011645B
CN115011645B CN202110702692.4A CN202110702692A CN115011645B CN 115011645 B CN115011645 B CN 115011645B CN 202110702692 A CN202110702692 A CN 202110702692A CN 115011645 B CN115011645 B CN 115011645B
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lysobacter
picolinic acid
xanthomonas
preventing
diseases
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CN115011645A (en
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刘凤权
佩德罗·拉沃尔达
赵杨扬
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Jiangsu Academy of Agricultural Sciences
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/78—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D213/79—Acids; Esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

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  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
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Abstract

The invention belongs to the technical field of biology, and particularly discloses application of picolinic acid in preparation of biopesticide for preventing and treating xanthomonas diseasesLysobactersp.) OH23, and separating and purifying picolinic acid from the culture solution of the strain, detecting the antagonistic activity of picolinic acid on specificity of xanthomonas by using a plate screening method, and in addition, the method for separating and purifying from the culture solution is simple, quick and efficient, is more friendly to the environment compared with chemical synthesis, has specific antibacterial activity on xanthomonas oryzae, and provides a favorable basis for developing novel biopesticide for preventing and treating xanthomonas oryzae diseases.

Description

Application of picolinic acid in preparation of biopesticide for preventing and treating xanthomonas diseases
Technical Field
The invention belongs to the technical field of biology, and particularly relates to application of picolinic acid in preparation of biological pesticides for preventing and treating xanthomonas diseases.
Background
Xanthomonas (Xanthomonas) is an important group of phytopathogenic bacteria that cause several tens of catastrophic diseases of crops worldwide. Rice is one of main grain crops in China, and rice bacterial leaf blight and leaf spot caused by two pathogenic varieties of Xanthomonas oryzae (X.oryzae pv. Oryzae) and leaf spot caused by leaf spot bacteria (X.oryzae pv. Oryzicola) severely restrict the yield and quality of rice. At present, chemical control of the two diseases is mainly performed by protective pesticides, and bacterial drug resistance caused by long-term application is a focus of food quality safety and ecological safety. Therefore, searching for new biocontrol microorganisms or metabolites thereof and developing the biocontrol microorganisms into ecologically safe and environment-friendly biopesticides will be the development direction of green disease control in the future.
Picolinic acid (Picolinic acid) is the end product of L-tryptophan in the kynurenine pathway, which is often found in a variety of biological media such as cell culture supernatants, serum and human milk. Picolinic acid may also be produced by microbial degradation of 2-aminophenol, nitrobenzene, catechol, anthranilic acid, 3-hydroxy-p-aminobenzoic acid, and the like. In addition, picolinic acid is an important intermediate for some organic synthetic drugs, herbicides and bactericides. Studies show that picolinic acid can inhibit the growth of kidney cells and the proliferation of T cells of mice and has antimicrobial activity, but no report that picolinic acid can inhibit the growth of Xanthomonas oryzae has been found yet.
Lysobacter (Lysobacter) belongs to the xanthomonas family of the γ -mutans class, and is receiving increasing attention as a new agricultural antibiotic source because it can produce a large amount of extracellular enzymes and active secondary metabolites, and is environmentally friendly. The applicant isolated a strain of Lysobacter (Lysobacter sp.) OH23 from rice rhizosphere soil and found that the bacterium had high-efficiency, specific antagonistic activity against xanthomonas sp. Therefore, how to use the lysobacter OH23 in biological pesticides is worth continuously exploring.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide the application of picolinic acid in preparing biopesticide for preventing and treating xanthomonas diseases, the picolinic acid is derived from Lysobacter (sp.) OH23, picolinic acid is separated and purified from a culture solution of the strain, the picolinic acid has specific antagonistic activity on xanthomonas by using a flat screen method, and in addition, the method for separating and purifying from the culture solution is simple, rapid and efficient, thereby providing a foundation for developing a novel biopesticide for preventing and treating the xanthomonas diseases of rice.
In order to solve the problems in the prior art, the invention adopts the following technical scheme:
picolinic acid, which is derived from a culture solution of Lysobacter (Lysobacter sp.) OH23, wherein the Lysobacter (Lysobacter sp.) OH23 is preserved in the China general microbiological culture collection center (CGMCC), with a preservation number: CGMCC No.13677, preservation date: address of deposit at 2 months 23 2017: the institute of microorganisms of national academy of sciences of China, no.1, no. 3, north Chen West Lu, the Korean region of Beijing.
The method for separating and purifying the culture solution of the picolinic acid from the Lysobacter (Lysobacter sp.) OH23 comprises the following steps:
step 1, fermenting Lysobacter (Lysobacter sp.) OH23 in NB culture medium culture, centrifuging and taking supernatant;
step 2, separating and purifying the supernatant by HPLC, wherein the mobile phase contains 0.06. 0.06N H 2 SO 4 The water with pH value of 5, flow rate of 0.8-1.2mL/min,30min,60 ℃, and ultraviolet absorption wavelength of 254nm to obtain picolinic acid.
As an improvement, the fermentation method in the step 1 is as follows: picking (Lysobacter sp.) OH23 colony, culturing in NB medium to obtain seed solution, transferring the seed solution into NB medium at 1% volume, culturing at 28deg.C and 180rpm for 48 hr, and centrifuging the fermentation broth at 10000rpm for 20min to obtain supernatant.
As an improvement, the chromatographic column used for separation and purification in the step 2 is C-18 (250X 9.4 mm),
the application of picolinic acid in preparing biopesticide for preventing and treating xanthomonas diseases.
The beneficial effects are that:
compared with the prior art, the application of picolinic acid in preparing biopesticide for preventing and treating xanthomonas diseases, disclosed by the invention, has the advantages that the function of producing picolinic acid is determined by culturing Lysobacter (Lysobacter sp.) OH23, and a method for separating and purifying picolinic acid by high-performance liquid chromatography is established. The antibacterial activity of picolinic acid is detected by a plate screening method, and the result shows that picolinic acid has specific antagonistic activity to xanthomonas and weaker inhibitory activity to other pathogenic bacteria. Moreover, the greenhouse test shows that the OH23 fermentation broth containing picolinic acid has good control effect on rice bacterial leaf blight, and can reduce the length of the lesion by 36.7%.
Drawings
FIG. 1 is a HPLC chromatogram of the supernatant of lysobacter OH 23;
FIG. 2 is a HPLC chromatogram of purified picolinic acid;
FIG. 3 is a mass spectrum of picolinic acid;
FIG. 4 shows the control effect of a Lysobacter (Lysobacter sp.) OH23 fermentation broth in a greenhouse on bacterial leaf blight of rice.
Detailed Description
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
EXAMPLE 1 activation of the lysobacter OH23 Strain and preparation of the fermentation supernatant
And taking out the lysobacter OH23 strain from the temperature of minus 80 ℃, streaking the strain on an NB solid culture medium, culturing the strain for 48 hours at the temperature of 28 ℃, picking a single colony on an NB liquid culture medium, and culturing the strain for 48 hours at the temperature of 28 ℃ and 180rpm to obtain seed liquid. The seed solution was inoculated to NB liquid medium at an inoculum size of 1%, and cultured at 28℃and 180rpm for 48 hours to give a fermentation broth. The fermentation broth was centrifuged at 10000rpm for 20min to obtain a fermentation broth supernatant.
EXAMPLE 2 isolation and purification of picolinic acid from the fermentation supernatant of lysobacter OH23
After the supernatant is filtered, the supernatant is separated and purified by HPLC, and the preparation conditions are as follows: the chromatographic column model is C-18 (250×9.4 mm), and the mobile phase contains 0.06. 0.06N H 2 SO 4 The flow rate of water with pH of 5 is 1.2mL/min, the column temperature is 60 ℃ and the ultraviolet detection wavelength is 254nm. The chromatographic peak with retention time of 17.2min is picolinic acid, the HPLC results are shown in FIG. 1, the HPLC detection of purified picolinic acid is shown in FIG. 2, molecular weight [ M+H ]] + The values are shown in figure 3.
EXAMPLE 3 antibacterial Activity of purified picolinic acid in the fermentation supernatant of Lysobacter OH23
Picolinic acid is added to NB medium, and then the picolinic acid-containing medium is poured into a petri dish to form picolinic acid with final concentrations of: 0. 50 μg/mL, 100 μg/mL, 250 μg/mL, 500 μg/mL, 750 μg/mL, 1000 μg/mL, 1250 μg/mL, 1500 μg/mL, 2000 μg/mL drug-containing petri dishes. After various bacteria were cultured to OD1.0, 4. Mu.L was dropped on a drug-containing petri dish and cultured at 28℃for 48 hours. The results were observed and recorded in table 1.
TABLE 1 bacteriostatic Effect of picolinic acid produced by Lysobacter OH23
As a result of observation, it was revealed that picolinic acid had MIC of 50. Mu.g/mL or less for Xanthomonas oryzae, MIC of 100. Mu.g/mL or less for other Xanthomonas oryzae, and antagonistic activity against other bacteria was weak (Table 1).
The method is simple, convenient and efficient, is more environment-friendly than chemical synthesis, and has important significance for preventing and controlling crop diseases caused by xanthomonas, and the specific antibacterial activity of the method for detecting the xanthomonas is more friendly than chemical synthesis. The antibacterial activity of picolinic acid is detected by a plate method, and the result shows that picolinic acid has specific antagonistic activity to xanthomonas and weaker inhibitory activity to other pathogenic bacteria.
Example 4 control Effect of OH23 fermentation liquor on bacterial leaf blight of Rice in greenhouse
Selecting rice plants in a 7-week growing period, and spraying 100mL of OH23 fermentation liquid containing picolinic acid on each 6-basin treated rice as a repetition. After one day, inoculation of bacterial leaf blight bacteria of rice is carried out by a leaf cutting method.
The specific method comprises the following steps: culturing bacterial leaf blight of rice to logarithmic phase, OD 600 Adjusting to 1.0, and cutting leaves and inoculating at the position 2cm away from the tip of the rice by using long-mouth surgical scissors to dip the bacterial liquid. After inoculation, the seeds are placed in an artificial greenhouse for cultivation, the set temperature is 28 ℃, and the relative humidity is 70%. After 14 days, the lesion length was measured and the results showed sprayingThe length of the lesion of the rice plant of the OH23 fermentation broth is reduced by 36.7% compared with the control (FIG. 4).
The results show that the OH23 fermentation liquor has better control effect on rice bacterial leaf blight in a greenhouse.
In the foregoing, the protection scope of the present invention is not limited to the preferred embodiments of the present invention, and any simple changes or equivalent substitutions of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention disclosed in the present invention fall within the protection scope of the present invention.

Claims (3)

1. The application of picolinic acid in preparing biopesticide for preventing and treating xanthomonas disease is characterized in that the picolinic acid is derived from lysobacterLysobactersp.) OH23, and the separation and purification method of picolinic acid comprises the following steps: step 1, lysobacterLysobactersp.) OH23 is fermented in NB culture medium, and supernatant is obtained by centrifugation; step 2, separating and purifying the supernatant by HPLC, wherein the mobile phase contains 0.06. 0.06N H 2 SO 4 Water with pH of 5, flow rate of 0.8-1.2mL/min,30min,60 deg.C, and ultraviolet absorption wavelength of 254nm to obtain picolinic acid; wherein the lysobacter is [ ]Lysobactersp.) OH23 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number: CGMCC No.13677, preservation date: address of deposit at 2 months 23 2017: the institute of microorganisms of national academy of sciences of China, no.1, no. 3, north Chen West Lu, the Korean region of Beijing.
2. The use according to claim 1, wherein the fermentation in step 1 is performed by: picking upLysobactersp.) OH23 colonies were cultured in NB medium to obtain seed solution, the seed solution was transferred to NB medium at 1% by volume, cultured at 28℃and 180rpm for 48 hours, and the fermentation broth was centrifuged at 10000rpm for 20 minutes to obtain supernatant.
3. The use according to claim 1, wherein the chromatographic column used for the separation and purification in step 2 is C-18.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2846610B2 (en) * 1995-11-02 1999-01-13 株式会社植物防御システム研究所 Screening method for elicitor that induces phytoalexin production in rice and rice disease controlling agent
KR20170054791A (en) * 2015-11-10 2017-05-18 대한민국(농촌진흥청장) Composition containing K252d derived from Streptomyces for rice bacterial blight suppressing activity
CN107400639A (en) * 2017-06-22 2017-11-28 江苏省农业科学院 A kind of molten bacillus of antagonism rice Xanthomonas and its separation method and application
CN108165498A (en) * 2018-01-23 2018-06-15 浙江师范大学 The Penicillium griseofulvum Pg-35 bacterial strains and its ferment filtrate of antagonism rice leaf spot bacteria and the application in the anti-smelting of plant disease
WO2019141980A1 (en) * 2018-01-17 2019-07-25 Globachem Nv Agricultural chemicals

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2846610B2 (en) * 1995-11-02 1999-01-13 株式会社植物防御システム研究所 Screening method for elicitor that induces phytoalexin production in rice and rice disease controlling agent
KR20170054791A (en) * 2015-11-10 2017-05-18 대한민국(농촌진흥청장) Composition containing K252d derived from Streptomyces for rice bacterial blight suppressing activity
CN107400639A (en) * 2017-06-22 2017-11-28 江苏省农业科学院 A kind of molten bacillus of antagonism rice Xanthomonas and its separation method and application
WO2019141980A1 (en) * 2018-01-17 2019-07-25 Globachem Nv Agricultural chemicals
CN108165498A (en) * 2018-01-23 2018-06-15 浙江师范大学 The Penicillium griseofulvum Pg-35 bacterial strains and its ferment filtrate of antagonism rice leaf spot bacteria and the application in the anti-smelting of plant disease

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