CN115011491A

CN115011491A - Culture process of candida utilis

Info

Publication number: CN115011491A
Application number: CN202210846706.4A
Authority: CN
Inventors: 黄贞胜
Original assignee: Xiamen Dachang Biotechnology Service Co ltd
Current assignee: Xiamen Dachang Biotechnology Service Co ltd
Priority date: 2022-07-06
Filing date: 2022-07-06
Publication date: 2022-09-06
Anticipated expiration: 2042-07-06
Also published as: CN115011491B

Abstract

The invention discloses a culture process of candida utilis, which comprises the following steps of S1: preparing solid culture medium, sterilizing with high pressure steam, and hot filling the upper part of the test tube on a glass rod to make the slant 1/2 of the culture medium. The optimized conditions of the invention are that the culture time is 13h, the temperature is 30 ℃, the rotating speed of a shaking table is 180r/min, the thallus density is highest when the carbon-nitrogen ratio (the mass ratio of glucose to peptone) is 3:1, and the long-acting effect is best, so the culture process guarantees the flora activity and the strain reproduction efficiency of the candida utilis on the basis of the parameters.

Description

Culture process of candida utilis

Technical Field

The invention relates to the technical field of strain culture, in particular to a culture process of candida utilis.

Background

Candida utilis (Candida utilis) is also called torula utilis or torula diet yeast. The contents of protein and vitamin B are higher than those of beer yeast, urea and nitric acid can be used as nitrogen sources, and the beer yeast can grow without adding any growth factor into a culture medium. It can utilize pentose and hexose, and can utilize waste sulfurous acid liquor of paper-making industry, molasses and wood hydrolysate to produce protein edible for human and animal.

The requirement of the activity of the strain and the number propagation of the strain on the culture process is high, the limitation on various parameters of the strain in the conventional culture is inaccurate, the activity of the strain and the propagation of the strain are not optimal, and the application of the strain is influenced.

Disclosure of Invention

Based on the technical problems in the background art, the invention provides a culture process of candida utilis.

The invention provides a culture process of candida utilis, which comprises the following steps:

s1: preparing a solid culture medium, sterilizing by high-pressure steam, and after sterilization, placing the upper part of a test tube on a glass rod while the solid culture medium to be prepared into an inclined plane to ensure that the length of the inclined plane of the culture medium is 1/2 of the length of the test tube;

s2: selecting a ring thallus from the candida utilis strain, placing the ring thallus into a prepared liquid culture medium, shaking uniformly, wherein the culture condition is 30-32 ℃, the rotating speed is 160-180r/min, and the culture time is 12-13 hours;

s3: performing plate pouring operation on the solid culture medium subjected to high-pressure steam sterilization treatment, taking a certain amount of bacterial liquid with a certain concentration from the liquid culture medium, injecting the bacterial liquid into the surface of the solid culture medium, and then uniformly coating the inoculated bacterial liquid on the surface of the culture medium by using a coating rod;

s4: the culture time is 36-48 hours, and after the operation, the taken plate can have the colony which is mistakenly fallen, so that the colony can be used for the later culture and seed-taking operation.

Preferably, the solid culture medium comprises 2-5% of glucose, 2-3% of peptone, 1-2% of yeast extract, 2-3% of agar and distilled water.

Preferably, the candida utilis is cultured in the culture medium for 13h at the temperature of 30 ℃, the rotating speed of a shaking table is 160r/min, and the thallus density is highest when the carbon-nitrogen ratio (the mass ratio of glucose to peptone) is about 3: 1.

Preferably, in the later stage of the candida utilis culture process, a yeast culture and proliferation device is further adopted for culture and proliferation, the device comprises a plurality of annular pipeline type culture cavities which are connected in series, and the opening and closing of a passage between every two adjacent culture cavities are controlled by a first baffle which can be opened and closed; the culture cavity comprises an outer wall and an inner wall sleeved in the outer wall; a first temperature adjusting layer is arranged between the outer wall and the inner wall; the outer wall and the inner wall are both made of heat conducting materials; the first temperature adjusting layer comprises a plurality of first semiconductor refrigerating sheets which are densely arranged; a hollow internal temperature adjusting rod is arranged at the center of the interior of the culture cavity along the length direction of the culture cavity; the inner temperature adjusting rod is filled with a solid-liquid phase change material; the internal temperature adjusting rod can be communicated with solid-liquid phase change materials circulating in the internal temperature adjusting rod from the outside; the phase transition temperature of the solid-liquid phase change material is within the safe temperature range of the yeast; a second temperature adjusting layer is arranged on the outer surface of the inner temperature adjusting rod; the second temperature adjusting layer also comprises a plurality of second semiconductor refrigerating sheets which are densely arranged; the rod body of the internal temperature adjusting rod is made of heat conducting materials; the outer wall of the culture cavity is provided with an air inlet pipe which can be opened and closed respectively and is used for supplementing required sterile gas and a feeding pipe which is used for supplementing required culture solution and thalli respectively along the tangential direction of the outer wall.

The two adjacent culture cavities are communicated through a connecting pipe, and the connecting pipe is tangent to the outer walls of the two culture cavities at the same time; the first baffle is hinged to the discharge port of the previous culture cavity, the previous culture cavity is kept in a circulation path while the connecting pipe is closed through rotation, and the circulation path of the previous culture cavity is closed while the connecting pipe is opened.

Wherein, a second baffle is hinged at the feed inlet of the next culture cavity; the second baffle realizes that the circulation path of the next culture cavity is kept while the connecting pipe is closed through rotation, and the circulation path of the next culture cavity is closed while the connecting pipe is opened.

The first baffle is of a porous structure, and a waterproof breathable film is covered on the surface of the first baffle; an air exhaust pipe is communicated with the connecting pipe to exhaust air outwards so as to keep negative pressure in the connecting pipe.

Wherein, a plurality of annular heat conducting fins are uniformly arranged on the outer surface of the outer wall; the annular heat conducting fins are perpendicular to the length direction of the outer wall.

Wherein, a plurality of culture cavities are integrally distributed up and down.

Wherein, the inner surface of the inner wall is provided with a spiral chamber line or a smooth bulge;

wherein the yeast culture proliferation method comprises the following steps:

injecting initial saccharomycete mixed culture solution into a culture cavity through a feed pipe at a certain speed, and injecting compressed sterile gas beneficial to saccharomycete culture into the culture cavity through an air inlet pipe in a continuous or pulse mode; the excess gas and waste in the culture cavity pass through the waterproof breathable film and the first baffle plate and are pumped out through the exhaust pipe, so that the stability of air pressure and the freshness of the gas in the culture cavity are guaranteed;

the temperature of the saccharomycete mixed culture solution in the culture cavity is accurately controlled from the inner direction and the outer direction through the first semiconductor refrigerating sheet and the second semiconductor refrigerating sheet to ensure efficient culture and proliferation of the saccharomycete, and the solid-liquid phase change material can fast eliminate redundant heat through circular flow and large specific heat capacity of the solid-liquid phase change material when the semiconductor refrigerating sheet breaks down or fails in emergency, can automatically balance the temperature of the saccharomycete mixed culture solution through the solid-liquid phase change characteristic of the solid-liquid phase change material, and can prevent the saccharomycete from dying in a large scale in a period of time;

after the set culture time is reached, the first baffle and the second baffle respectively rotate in the previous culture cavity and the next culture cavity, so that flowing yeast culture solution enters the next culture cavity from the previous culture cavity through the connecting pipe under the action of inertia, after the culture is completed, the first baffle and the second baffle are closed again, the previous culture cavity is continuously injected with new yeast mixed culture solution or the previous yeast mixed culture solution in the previous culture cavity, and the next culture cavity continuously cultures the previous yeast culture solution until the culture and proliferation targets are reached after the N-channel culture and then the culture is discharged.

The beneficial effects of the invention are as follows:

1. the invention analyzes that the influence of each factor on the growth of the yeast is not obvious (p is more than 0.05) through statistics, the final optimized conditions are that the culture time is 13h, the temperature is 30 ℃, the rotating speed of a shaking table is 180r/min, the thallus density is highest when the carbon-nitrogen ratio (the mass ratio of glucose to peptone) is 3:1, namely, the long-acting effect is best, so the culture process guarantees the flora activity and the strain reproduction efficiency of the candida utilis based on the parameters.

2. The invention is provided with the multi-stage series-connection culture cavity capable of circularly and dynamically culturing, compared with the traditional method of static culture proliferation by adopting the tank body, the invention can realize continuous pulsation and dynamic culture proliferation, obviously improve the production efficiency and the production continuity, reduce the retention time of thalli between the completion of culture proliferation and the next procedure, and improve the survival rate and the culture efficiency of thalli finished products; the culture and proliferation process of the thalli is divided into a plurality of sections to be continuously carried out, thereby realizing continuity and controllability, reducing the influence of partial unqualified thalli on the whole and reducing the abandonment rate.

3. The culture cavity provided by the invention adopts the semiconductor refrigerating sheets arranged inside and outside, so that the contact area with the yeast mixed culture solution can be increased, the thermal conductivity and the conduction uniformity are improved, the temperature can be accurately and bidirectionally controlled by controlling the current magnitude and the direction, the yeast is ensured to be at the optimum culture temperature, the efficient culture proliferation of the yeast is ensured, meanwhile, the solid-liquid phase change material can rapidly remove redundant heat when the semiconductor refrigerating sheet breaks down or fails under emergency conditions through circulating flow and larger specific heat capacity per se, the temperature of the yeast mixed culture solution can be automatically balanced through the self solid-liquid phase change characteristic, and the large-scale death of the yeast can be avoided within a period of time.

4. According to the invention, by utilizing the characteristic that the air inlet pipe and the feed pipe are both tangent to the culture cavity, the yeast and the culture solution can be fed into the culture cavity and the gas required by the yeast can be supplemented according to the design and calculation structure, and by utilizing the impacted liquid inlet and air inlet, the power of circular rotation can be provided for the mixed culture solution in the culture cavity, so that the yeast mixed culture solution can circularly flow in the culture cavity, meanwhile, the spiral chamber lines or the smooth bulges on the inner surface of the inner wall can enable the mixed culture solution to have stronger turbulent flow, promote the substance exchange and gas dissolution of the yeast mixed culture solution, and facilitate the further update of the culture solution and the gas and the uniformity of the temperature; and redundant gas and waste in the culture cavity penetrate through the waterproof breathable film and the first baffle plate and are pumped out through the exhaust tube, so that the stability of air pressure and the freshness of the gas in the culture cavity are guaranteed.

Drawings

FIG. 1 is a schematic process diagram of the present invention;

FIG. 2 is a schematic view showing the construction of a yeast culturing and proliferating device of the present invention;

FIG. 3 is a schematic radial cross-sectional view of a temperature adjustment rod in the present invention;

FIG. 4 is a schematic cross-sectional view of a second baffle of the present invention.

In the figure: 1. a culture cavity; 11. an outer wall; 12. an inner wall; 13. a first temperature adjustment layer; 14. a first semiconductor refrigeration chip; 15. an internal temperature adjusting rod; 16. a solid-liquid phase change material; 17. a second temperature adjustment layer; 18. a second semiconductor refrigeration chip; 2. a first baffle plate; 21. a waterproof breathable film; 22. an exhaust pipe; 3. an air inlet pipe; 4. a feed pipe; 6. a connecting pipe; 7. a second baffle.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1, a process for culturing candida utilis comprises the following steps:

Wherein, the solid culture medium comprises 2-5% of glucose, 2-3% of peptone, 1-2% of yeast extract powder, 2-3% of agar and distilled water.

Wherein the candida utilis is cultured in a culture medium for 13h at the temperature of 30 ℃, the rotating speed of a shaking table is 160r/min, and the thallus density is highest when the carbon-nitrogen ratio (the mass ratio of glucose to peptone) is about 3: 1.

Specifically, in the present invention, the yeast extract mature glucose medium (also referred to as YPD medium) is prepared from the following raw materials: 2-5% of glucose, 2-3% of peptone, 1-2% of yeast extract powder and distilled water; the solid culture medium is prepared by adding agar 2-3% based on liquid culture medium.

The amount of the culture medium to be dispensed is determined as the case may be, and it is preferable that the solid culture medium in the test tube is 1/5 having a tube height, and that the volume of the culture medium to be filled in the triangular flask is 1/2.

Then the prepared culture medium is sterilized by high pressure steam, the method is that the object to be sterilized is put in a high pressure steam sterilizing pot, the boiling point of water rises under high pressure, thereby causing the temperature of steam to rise, and high temperature is generated to achieve the aim of killing the mixed bacteria.

Since the culture medium contains saccharides, 7X 10 is generally used ⁴ Pa (gauge pressure) (115 deg.C) for 20-30 min to avoid high temperature of saccharideAnd decomposed.

Since autoclaving kills microorganisms by raising the temperature of the steam by increasing the pressure of the steam, the air in the pan should be exhausted as much as possible before pressurization.

After the sterilization is finished, the upper part of the test tube is padded on a glass rod when the solid culture medium needing to be made into the inclined plane is hot, so that the length of the inclined plane of the culture medium is 1/2 of the length of the test tube.

Shake cultivation: selecting a ring of thallus from the purchased strains, placing the thallus into the prepared liquid culture medium, and shaking up.

Placing the triangular flask containing the liquid culture medium in a shaking incubator for shake cultivation under the conditions of 30-32 ℃, the rotation speed of 160-180r/min and the cultivation time of 12-13 hours.

Under the culture conditions, the thalli can grow rapidly and are in logarithmic growth phase, and a foundation is laid for later culture.

Constant temperature and humidity culture: and (3) performing plate pouring operation on the solid culture medium subjected to the high-pressure steam sterilization treatment, pouring the prepared solid culture medium into the plate while the prepared solid culture medium is hot, and then cooling all the culture mediums in the plate to room temperature and solidifying the culture mediums.

At this time, an inoculation operation is performed, a certain amount of bacterial liquid with a certain concentration is taken out from the liquid culture medium and injected to the surface of the solid culture medium, and then the inoculated bacterial liquid is uniformly spread on the surface of the culture medium by using a coating rod.

And finally, putting the plate into a constant-temperature constant-humidity incubator for dark culture for 36-48 hours, and taking out the plate to obtain the bacterial colony which is mistakenly dropped on the plate for later culture and seed taking operation.

After the culture, 1mL of the bacterial solution was taken out, diluted 5-fold with distilled water, and the absorbance at 600nm was measured with an ultraviolet-visible spectrophotometer as an initial value.

And placing the triangular flask containing the liquid culture medium in a constant-temperature shaking incubator at 30 ℃ and 160r/min for shaking culture, measuring the absorbance value (OD value) of the bacterial liquid every 1 hour by the same method, and continuously measuring for 24 hours.

From the data, yeast growth curves (absorbance/time) were made and log phase was found.

In the aspect of carbon source, three raw materials are selected, namely glucose, maltose and sucrose; three raw materials, namely peptone, ammonium sulfate and ammonium chloride are also selected from the aspect of nitrogen sources.

The yeast has a certain sequence for utilizing different carbon sources.

In the intermittent fermentation, glucose and fructose are utilized firstly, the yeast can ferment maltose only when the culture medium does not contain glucose and fructose, and the yeast can utilize the carbon source firstly when the carbon source can ensure the maximum growth and reproduction speed of the yeast.

And nitrogen source, yeast can only utilize two forms of nitrogen: ammonia and organic nitrogen.

Among the components of the microbial culture medium, the most commonly used organic ammonia sources are yeast extract and various peptones obtained by enzymatic digestion of animal and plant proteins.

According to the optimal growth temperature of Candida virginiana, the culture temperature was set to three groups of conditions, 28 ℃, 30 ℃ and 32 ℃ respectively, according to the previous optimized conditions.

In the research of the dissolved oxygen condition in the culture, the strain Candida virgata is facultative anaerobe and is more suitable for the culture under aerobic conditions.

According to the determination of the influence conditions of the rotating speed of the shaking table, the rotating speed of the shaking table incubator is set to three groups of conditions of 140r/min, 160r/min and 180 r/min.

And respectively carrying out single factor and orthogonal treatment on the three factors and the three levels, and comparing and analyzing the growth condition of the saccharomycetes by each influencing factor so as to obtain the optimal condition for regrowth after mutagenesis.

The final optimized conditions are that the thallus density is highest when the culture time is 13h, the temperature is 30 ℃, the rotating speed of a shaking table is 160r/min and the carbon-nitrogen ratio (the mass ratio of glucose to peptone) is about 3:1, namely the best long-acting effect is generated.

Further, referring to fig. 2 to 4, in order to enable candida utilis to proliferate rapidly, a yeast culture proliferation device is designed, the device comprises a plurality of annular pipeline type culture cavities 1 which are connected in series, and the opening and closing of a passage between two adjacent culture cavities 1 are controlled by an openable and closable first baffle plate 2; the culture cavity 1 comprises an outer wall 11 and an inner wall 12 sleeved in the outer wall 11; a first temperature adjusting layer 13 is arranged between the outer wall 11 and the inner wall 12; the outer wall 11 and the inner wall 12 are both made of heat conducting materials; the first temperature regulation layer 13 includes a plurality of first semiconductor chilling plates 14 arranged densely; a hollow internal temperature adjusting rod 15 is arranged at the center of the interior of the culture cavity 1 along the length direction; the inside of the inner temperature adjusting rod 15 is filled with a solid-liquid phase change material 16; the internal temperature adjusting rod 15 can be communicated with the solid-liquid phase change material 16 circulating in the internal part from the outside; the phase transition temperature of the solid-liquid phase-change material 16 is within the safe temperature range of yeast; the outer surface of the inner temperature adjusting rod 15 is provided with a second temperature adjusting layer 17; the second temperature regulation layer 17 also includes a plurality of second semiconductor chilling plates 18 arranged densely; the rod body of the inner temperature adjusting rod 15 is made of heat conducting material; the outer wall 11 of the culture chamber 1 is provided with an air inlet pipe 3 and a feeding pipe 4, which are respectively openable and closable and are used for supplementing the required sterile gas and supplementing the required culture solution and thalli, along the tangential direction.

Further, two adjacent culture cavities 1 are communicated through a connecting pipe 6, and the connecting pipe 6 is tangent to the outer walls 11 of the two culture cavities 1; first baffle 2 articulates the discharge gate department of one preceding cultivation cavity 1, realizes making one preceding cultivation cavity 1 keep circulation path when closed connecting pipe 6 through rotating, and makes one preceding cultivation cavity 1's circulation path closed when opening connecting pipe 6.

Further, a second baffle 7 is hinged at the feed inlet of the subsequent culture cavity 1; the second shutter 7 is rotated to close the connection pipe 6 and maintain the circulation path of the succeeding culture chamber 1, and to open the connection pipe 6 and close the circulation path of the succeeding culture chamber 1.

Further, the first baffle 2 is of a porous structure, and the surface of the first baffle is covered with a waterproof breathable film 21; an air exhaust tube 22 is connected with the connecting tube 6 to exhaust air outwards so as to keep negative pressure in the connecting tube 6.

Furthermore, a plurality of annular heat conducting fins are uniformly arranged on the outer surface of the outer wall 11; the annular heat conducting fins are perpendicular to the length direction of the outer wall 11.

Further, a plurality of culture chambers 1 are integrally distributed up and down.

Further, the inner surface of the inner wall 12 is provided with a spiral chamber line or a smooth bulge;

the yeast culture proliferation method comprises the following steps:

firstly, injecting initial yeast mixed culture solution into a culture cavity 1 through a feed pipe 4 at a certain speed, simultaneously injecting compressed sterile gas beneficial to yeast culture into the culture cavity 1 through an air inlet pipe 3 in a continuous or pulse mode, wherein the air inlet pipe 3 and the feed pipe 4 are both tangent to the culture cavity 1, so that the yeast mixed culture solution can circularly flow in the culture cavity 1, and meanwhile, a spiral chamber line or smooth bulge on the inner surface of an inner wall 12 can enable the mixed culture solution to have stronger turbulence, promote the material exchange and gas dissolution of the yeast mixed culture solution, and facilitate further culture solution and gas renewal and temperature uniformity; the excess gas and waste in the culture cavity 1 pass through the waterproof breathable film 21 and the first baffle 2 and are pumped out through the exhaust tube 22, so that the stability of the air pressure and the freshness of the gas in the culture cavity 1 are guaranteed;

the temperature of the yeast mixed culture solution in the culture cavity 1 is accurately controlled from the inner direction and the outer direction through the first semiconductor refrigerating sheet 14 and the second semiconductor refrigerating sheet 18, so that efficient culture and proliferation of the yeast are guaranteed, the solid-liquid phase change material 16 can quickly remove redundant heat when the semiconductor refrigerating sheet breaks down or fails in emergency due to circular flow and large specific heat capacity of the solid-liquid phase change material, the temperature of the yeast mixed culture solution can be automatically balanced through the solid-liquid phase change characteristic of the solid-liquid phase change material, and large-scale death of the yeast can be avoided within a period of time;

after setting for the cultivation time and reaching, first baffle 2 and second baffle 7 cultivate cavity 1 and one back respectively and cultivate the cavity 1 internal rotation forward, make flowing yeast culture solution under inertial action from last one cultivate cavity 1 through connecting pipe 6 get into one down cultivate in the cavity 1, after accomplishing, first baffle 2 and second baffle 7 are closed again, one is cultivateed cavity 1 and continues to inject new yeast mixed culture solution or one more preceding yeast mixed culture solution of cultivateing cavity 1, one is cultivateed cavity 1 and continues to cultivate the yeast culture solution before one before, discharge after reaching the cultivation proliferation target after carrying out N cultivateings.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A culture process of candida utilis is characterized by comprising the following steps of:

s1: preparing a solid culture medium, sterilizing the solid culture medium by high-pressure steam, and after the solid culture medium is sterilized, placing the upper part of a test tube on a glass rod while the solid culture medium is hot so that the length of the inclined plane of the culture medium is 1/2 of the length of the test tube;

2. The process of claim 1, wherein the solid medium comprises glucose 2-5 wt%, peptone 2-3 wt%, yeast extract 1-2 wt%, agar 2-3 wt%, and distilled water.

3. The Candida utilis culture process according to claim 1, wherein the Candida utilis is cultured in the culture medium for 13 hours at a temperature of 30 ℃, at a table rotation speed of 160r/min and at a carbon-nitrogen ratio (glucose to peptone mass ratio) of about 3:1, and the density of the cells is highest.

4. The Candida utilis culture process according to any one of claims 1 to 3, further comprising a yeast culture proliferation process, wherein the culture proliferation process is carried out by using a yeast culture proliferation device, the device comprises a plurality of annular pipeline type culture cavities (1) which are connected in series, and the opening and closing of a passage between two adjacent culture cavities (1) are controlled by an openable and closable first baffle plate (2); the culture cavity (1) comprises an outer wall (11) and an inner wall (12) sleeved in the outer wall (11); a first temperature adjusting layer (13) is arranged between the outer wall (11) and the inner wall (12); the outer wall (11) and the inner wall (12) are both made of heat conducting materials; the first temperature adjusting layer (13) comprises a plurality of first semiconductor refrigeration sheets (14) which are densely arranged; a hollow internal temperature adjusting rod (15) is arranged at the center of the interior of the culture cavity (1) along the length direction of the culture cavity; the inner temperature adjusting rod (15) is filled with a solid-liquid phase change material (16); the inner temperature adjusting rod (15) can be communicated with a solid-liquid phase change material (16) circulating in the inner part from the outside; the phase transition temperature of the solid-liquid phase-change material (16) is within the safe temperature range of yeast; a second temperature adjusting layer (17) is arranged on the outer surface of the inner temperature adjusting rod (15); the second temperature adjusting layer (17) also comprises a plurality of second semiconductor chilling plates (18) which are densely arranged; the rod body of the inner temperature adjusting rod (15) is made of heat conducting materials; the outer wall (11) of the culture cavity body (1) is provided with an air inlet pipe (3) which can be opened and closed respectively and is used for supplementing the required sterile gas and a feeding pipe (4) which is used for supplementing the required culture solution and the thallus along the tangential direction of the outer wall.

5. The Candida utilis culture process according to claim 4, wherein two adjacent culture chambers (1) are communicated through a connecting pipe (6), and the connecting pipe (6) is tangential to the outer walls (11) of the two culture chambers (1); first baffle (2) hinge the discharge gate department of one preceding cultivation cavity (1), realize through rotating that make one preceding cultivation cavity (1) keep circulation route when closed connecting pipe (6), and make the circulation route closure of one preceding cultivation cavity (1) when opening connecting pipe (6).

6. The Candida utilis culture process according to claim 5, wherein the second baffle (7) is hinged to the feed inlet of the subsequent culture chamber (1); the second baffle (7) enables the subsequent culture cavity (1) to keep a circulation path while closing the connecting pipe (6) through rotation, and enables the circulation path of the subsequent culture cavity (1) to be closed while opening the connecting pipe (6).

7. The Candida utilis culture process according to claim 6, wherein said first baffle (2) is a porous structure and covered with a waterproof and breathable film (21); an air extraction pipe (22) is communicated with the connecting pipe (6) to extract air outwards so as to keep negative pressure in the connecting pipe (6).

8. The Candida utilis culture process according to claim 7, wherein the external surface of said outer wall (11) has a plurality of annular heat-conducting fins uniformly; the annular heat conducting fins are vertical to the length direction of the outer wall (11); the inner surface of the inner wall (12) is provided with a spiral chamber line or a smooth bulge.

9. The Candida utilis culture process according to claim 8, wherein a plurality of culture chambers (1) are integrally distributed in the vertical direction.

10. The Candida utilis culture process according to claim 9, wherein the yeast culture proliferation method is:

injecting initial yeast mixed culture solution into a culture cavity (1) through a feed pipe (4) at a certain speed, and simultaneously injecting compressed sterile gas beneficial to yeast culture into the culture cavity (1) through an air inlet pipe (3) in a continuous or pulse mode, wherein the air inlet pipe (3) and the feed pipe (4) are tangent to the culture cavity (1), so that the yeast mixed culture solution can circularly flow in the culture cavity (1), and meanwhile, a spiral chamber line or smooth bulge on the inner surface of an inner wall (12) can enable the mixed culture solution to have stronger turbulence, promote the material exchange and gas dissolution of the yeast mixed culture solution, and facilitate the update of the culture solution and the gas in the culture solution and the uniformity of the temperature; redundant gas and waste in the culture cavity (1) pass through the waterproof breathable film (21) and the first baffle (2) and are pumped out through the exhaust pipe (22), so that the stability of air pressure and the freshness of the gas in the culture cavity (1) are guaranteed;

the temperature of the yeast mixed culture solution in the culture cavity (1) is accurately controlled from the inner direction and the outer direction through the first semiconductor refrigerating piece (14) and the second semiconductor refrigerating piece (18) to ensure efficient culture and proliferation of yeast, the solid-liquid phase change material (16) can rapidly remove redundant heat when the semiconductor refrigerating piece fails or fails under emergency conditions through circulating flow and large specific heat capacity of the solid-liquid phase change material, the temperature of the yeast mixed culture solution can be automatically balanced through the solid-liquid phase change characteristic of the solid-liquid phase change material, and large-scale death of the yeast can be avoided within a period of time;

after the set culture time is reached, the first baffle (2) and the second baffle (7) respectively rotate in the previous culture cavity (1) and the next culture cavity (1), so that flowing yeast culture solution enters the next culture cavity (1) from the previous culture cavity (1) through the connecting pipe (6) under the action of inertia, after the culture is completed, the first baffle (2) and the second baffle (7) are closed again, the previous culture cavity (1) continues to be filled with new yeast mixed culture solution or the previous yeast mixed culture solution of the previous culture cavity (1), the next culture cavity (1) continues to culture the previous yeast culture solution, and the previous yeast culture solution is discharged until a culture proliferation target is reached after N-channel culture.