CN115011479B - Preparation method of probiotics - Google Patents

Preparation method of probiotics Download PDF

Info

Publication number
CN115011479B
CN115011479B CN202210834892.XA CN202210834892A CN115011479B CN 115011479 B CN115011479 B CN 115011479B CN 202210834892 A CN202210834892 A CN 202210834892A CN 115011479 B CN115011479 B CN 115011479B
Authority
CN
China
Prior art keywords
collecting
sleeve
barrel
control
groove
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210834892.XA
Other languages
Chinese (zh)
Other versions
CN115011479A (en
Inventor
殷雷
王一凡
刘毅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Heilongjiang Baicai Agricultural Technology Co ltd
Original Assignee
Heilongjiang Baicai Agricultural Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Heilongjiang Baicai Agricultural Technology Co ltd filed Critical Heilongjiang Baicai Agricultural Technology Co ltd
Priority to CN202210834892.XA priority Critical patent/CN115011479B/en
Publication of CN115011479A publication Critical patent/CN115011479A/en
Application granted granted Critical
Publication of CN115011479B publication Critical patent/CN115011479B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/05—Means for pre-treatment of biological substances by centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a preparation method of probiotics. According to the preparation method of the probiotics, at least two tubes are arranged, the tubes are switched between the centrifugal position and the collecting position by utilizing the sleeve, so that the centrifugal separation operation of the probiotics on the tubes at the centrifugal position can be continuously carried out in the process of collecting the probiotics mud adsorbed on the inner wall of the collecting position tube through centrifugal separation, and the preparation efficiency of the probiotics is improved.

Description

Preparation method of probiotics
Technical Field
The invention relates to the technical field of probiotics, in particular to a preparation method of probiotics.
Background
Probiotics are microorganisms that have medical and health care effects on the human and animal body by improving the balance of microorganisms and enzymes of the body, stimulating specific or non-specific immune mechanisms. At present, when probiotics are prepared, generally, a probiotic bacterial strain is placed into a culture medium for culture fermentation, the temperature is reduced after fermentation for a period of time to obtain a probiotic fermentation liquid, then the fermentation liquid is taken out, centrifugal separation treatment is carried out on the fermentation liquid, supernatant fluid is discarded to obtain probiotic bacterial mud, then the probiotic bacterial mud and a protective agent are mixed to prepare an emulsion, and then vacuum freeze-drying treatment is carried out on the emulsion to support freeze-dried probiotic powder.
In the process of preparing probiotics, since fermentation broth is required to be subjected to centrifugal treatment, a centrifugal machine is generally used for centrifugal work, but an intermediate rotary tube barrel is required to be taken down after the centrifugation is completed by the conventional centrifugal machine, and then probiotic bacterial mud adsorbed in the tube barrel is scraped by a manual scraping mode, so that the process is completed manually, the workload is large, the efficiency is low, and the process is specifically shown as follows: (1) because the centrifugal machine has a complex structure, the manual removal of the pipe barrel is complicated, and a lot of manpower is needed; (2) when the pipe barrel is taken down, the centrifuge must be stopped, and the production cycle is delayed; (3) the workload of manually scraping the probiotic bacterial sludge is large, and the probiotic bacterial sludge is required to be collected after scraping; (4) cleaning of the scraping tool is required to prevent contamination of the subsequently scraped probiotic bacterial sludge.
Disclosure of Invention
In view of the above, the present invention discloses a method for preparing probiotics to overcome or at least partially solve the above problems.
The preparation method of the probiotics adopts probiotic processing equipment to carry out preparation operation of the probiotics, wherein the probiotic processing equipment comprises a sleeve, a tube barrel, an upper plug and a lower plug; the sleeve is provided with at least two tubes, and can drive the tubes to switch positions between a centrifugal position and a collecting position, and the tubes positioned in the centrifugal position can rotate; the upper plug and the lower plug are positioned at the centrifugal position and can be detachably connected with the upper end and the lower end of the pipe barrel positioned at the centrifugal position respectively; the upper plug is provided with an upper runner, one end of the upper runner is communicated with the upper end of the pipe barrel, and the other end of the upper runner is communicated with the waste barrel; the lower plug is provided with a lower runner, one end of the lower runner is communicated with the lower end of the pipe barrel, and the other end of the lower runner is selectively communicated with a fermentation liquid source and a collecting barrel;
the preparation method of the probiotics specifically comprises the following steps:
step S1, preparing: the upper plug and the lower plug are respectively connected with the upper end and the lower end of the pipe barrel of the centrifugal position, the waste material barrel is communicated with the upper end of the pipe barrel through an upper flow passage in the upper plug, and a fermentation liquid source is communicated with the lower end of the pipe barrel through a lower flow passage in the lower plug;
step S2, feeding and centrifuging: the tube barrel positioned at the centrifugal position is driven to rotate, meanwhile, fermentation liquor of a fermentation liquor source is conveyed into the tube barrel through the lower flow passage and rotates along with the tube barrel, so that the fermentation liquor is centrifugally separated, probiotic bacterial sludge in the fermentation liquor is adsorbed on the inner wall of the tube barrel, and supernatant produced after the fermentation liquor is subjected to centrifugal action flows into the waste material barrel through the upper flow passage;
step S3, exchanging the tube barrel: stopping the rotation of the pipe barrel after the feeding centrifugation in the step S2 is completed, controlling the upper plug and the lower plug to release the connection with the pipe barrel, controlling the sleeve to move the pipe barrel with the completed feeding centrifugation to a collecting position and move the other pipe barrel to a centrifugation position, controlling the upper plug and the lower plug to be connected with the upper end and the lower end of the pipe barrel in the centrifugation position again, and controlling the pipe barrel in the centrifugation position to carry out feeding centrifugation operation according to the step S2;
s4, collecting probiotic bacterial sludge; and collecting probiotic bacterial sludge from the tube at the collecting position.
Preferably, the probiotic processing device comprises an intermediate sleeve and a spool; the middle shaft sleeve is connected with the sleeve, the middle shaft sleeve can drive the sleeve to move along the height direction of the pipe barrel, and the sleeve can rotate relative to the middle shaft sleeve; the spool is in sliding connection with the middle shaft sleeve, and can synchronously move along with the middle shaft sleeve and move relative to the middle shaft sleeve; the lower plug and the upper plug are respectively connected with the pipe barrel in an inserting way, and the upper plug is connected with the spool.
Preferably, the probiotic processing device further comprises a main support shaft and a replacement telescopic rod; the middle shaft sleeve is in sliding connection with the main support shaft along the vertical direction, one end of the exchange telescopic rod is connected with the main support shaft, and the other end of the exchange telescopic rod is connected with the middle shaft sleeve so as to drive the middle shaft sleeve to reciprocate linearly relative to the main support shaft.
Preferably, the sleeve is rotatably connected with the middle shaft sleeve, and the main support shaft is arranged outside the sleeve; the outer surface of the sleeve is provided with at least two axial step grooves and at least two spiral grooves, the step grooves are straight grooves and are arranged at intervals along the circumferential direction of the sleeve, the upper half part of each step groove is a deep groove along the axial direction of the sleeve, and the lower half part of each step groove is a shallow groove; one spiral groove is arranged between two adjacent step grooves, the upper end of the spiral groove is communicated with the lower end of a deep groove in one step groove, and the lower end of the spiral groove is communicated with the lower end of a shallow groove in the other step groove; the main support shaft is provided with a guide pin, one end of the guide pin is connected with the main support shaft, and the other end of the guide pin extends out to the step groove or the spiral groove and can relatively move along the step groove and the spiral groove;
in the process that the sleeve moves relative to the main support shaft, when the guide pin moves from the upper end of the deep groove to the lower end of the deep groove, the pipe barrel moves to a position which is disconnected with the upper plug and the lower plug, and when the guide pin moves to the lower end of the shallow groove in the adjacent step groove along the spiral groove, the pipe barrel completes the transfer between the centrifugal position and the collecting position.
Preferably, the probiotic processing device further comprises a first rack, a second rack and a gear; the first rack is fixed on the spool, the second rack is fixed on the main support shaft, the gear is rotatably arranged on the middle shaft sleeve, and the gear is simultaneously meshed with the first rack and the second rack.
Preferably, the probiotic processing device further comprises a collecting telescopic rod and a collecting piston; the collecting telescopic rod is positioned at a collecting position, the collecting piston is positioned at the extending end of the collecting telescopic rod, and the outer diameter size of the collecting piston corresponds to the inner diameter size of the pipe barrel; the collection telescoping rod is capable of driving the collection piston through the tube in a collection position.
Preferably, the probiotic processing device further comprises a spray head; the spray head is positioned at the lower end of the tube in the collecting position and can spray high-pressure gas to the collecting piston;
the preparation method of the probiotics further comprises the step S5 of cleaning the collecting piston: after the collecting piston passes through the pipe barrel, the spray head is controlled to output high-pressure gas to the collecting piston, so that surface cleaning of the collecting piston is formed.
Preferably, the probiotic processing device further comprises a collection chamber, an air bag and a control valve; the collecting cavity is positioned at the collecting position, the air bag is provided with an air inlet and an air outlet, the spray head is positioned in the collecting cavity, and the control valve is positioned between the collecting cavity and the air bag; in the process that the collecting piston moves along the pipe barrel to the direction close to the collecting cavity, the control valve is switched to the collecting cavity to be communicated with the air inlet, and the gas in the collecting cavity is stored to the air bag in a boosting mode; when the collecting piston moves to a position close to the spray head, the control valve is switched to the air outlet to be communicated with the spray head, and high-pressure gas in the air bag is output to the spray head.
Preferably, the control valve comprises a control plate, a control rod and a control spring, wherein a first control hole, a second control hole and a control groove are formed in the control plate; the control panel is arranged between the collecting cavity and the air bag in a sliding manner; the control spring is connected with the control board to drive the control board to move to the state that the first control hole is blocked, the second control hole is communicated with the collecting cavity and the air inlet, and the control groove is communicated with the outside atmosphere; one end of the control rod is connected with the control plate, the other end of the control rod extends into the collecting cavity and is positioned at the lower end of the pipe barrel, the collecting piston penetrates through the pipe barrel and then can contact with the control rod to drive the control rod to overcome the acting force of the control spring so as to drive the control plate to move to the first control hole to be communicated with the spray head and the air outlet, the second control hole is in a blocking state, and the collecting cavity is communicated with the outside atmosphere by the control groove.
Preferably, the probiotic processing device further comprises a door panel; the door plate is movably connected with the collecting cavity and used for controlling the opening and closing of the collecting cavity.
The preparation method of the probiotics is adopted for preparing the probiotics, and has the following beneficial technical effects:
1. in the preparation method of the probiotics, at least two tubes are arranged, the tubes are switched between the centrifugal position and the collecting position by utilizing the sleeve, so that the centrifugal separation of the probiotics in the other tube can be continuously carried out in the process of collecting the probiotics mud adsorbed in the tubes through the centrifugal separation, and the preparation efficiency of the probiotics is improved.
2. In the preparation method of the probiotics, under the condition that the upper plug and the lower plug are connected with the pipe barrel in an inserting way, the first rack, the second rack and the gear are arranged between the middle shaft sleeve and the spool, so that the spool can move in the same direction relative to the middle shaft sleeve in the vertical direction relative to the main supporting shaft when the middle shaft sleeve is driven by the telescopic rod in a changing way, and the switching efficiency of the pipe barrel between the centrifugal position and the collecting position is improved.
3. According to the preparation method of the probiotics, the guide pin, the step groove and the spiral groove are arranged between the main support shaft and the sleeve, so that the sleeve is rotated relative to the main support shaft in the process of changing the telescopic rod to drive the middle shaft sleeve to drive the spool to move relative to the main support shaft in the vertical direction, and automatic rotation switching of the tube between the centrifugal position and the collecting position is realized, manual disassembly and assembly transfer is omitted, labor is saved, and switching efficiency is improved.
4. According to the preparation method of the probiotics, disclosed by the invention, the collection piston is driven by the collection telescopic rod to pass through the tube barrel at the collection position, so that the pushing-out of the probiotics mud on the inner wall of the tube barrel can be rapidly completed instead of manpower, and the collection speed of the probiotics mud is improved.
5. According to the preparation method of the probiotics, the spray head is arranged in the collecting cavity, and the air bag communicated with the collecting cavity is controlled by the control valve, so that high-pressure gas can be filled into the air bag in the process that the collecting piston pushes out the probiotics mud, after the collecting piston completes pushing out the probiotics mud, the automatic control valve is switched to the air bag to output the high-pressure gas to the collecting piston through the spray head, automatic cleaning operation of the collecting piston is achieved, labor is saved, pollution to the probiotics mud is prevented, and the preparation quality of the probiotics mud is improved.
Drawings
FIG. 1 is a schematic cross-sectional view of a probiotic processing device according to the present embodiment;
FIG. 2 is a schematic view of the partial structure at I in FIG. 1;
FIG. 3 is a schematic view of the partial structure at II in FIG. 1;
FIG. 4 is a schematic view of the partial structure at III in FIG. 1;
FIG. 5 is a schematic view along A-A in FIG. 4;
FIG. 6 is a schematic view of a first view of a sleeve according to the present embodiment;
fig. 7 is a schematic partial structure of the sleeve according to the second view angle of the present embodiment.
Detailed Description
The technical scheme of the invention is further described in detail below with reference to the accompanying drawings and the examples.
As shown in fig. 1 to 7, this embodiment discloses a probiotic processing device, which includes a sleeve 1, a tube 2, an upper plug 3 and a lower plug 4. Wherein, be equipped with two tubes 2 on sleeve 1 to can drive two tubes 2 and carry out the position between centrifugation position and collection position and switch, the tube 2 that is located the centrifugation position then can carry out the rotation. The upper and lower plugs 3 and 4 are located at the centrifugal position and can be detachably connected with the upper and lower ends of the tube 2 located at the centrifugal position, respectively. Meanwhile, an upper runner 5 is arranged on the upper plug 3, one end of the upper runner 5 is communicated with the upper end of the pipe barrel 2, and the other end of the upper runner is communicated with an externally connected waste barrel; the lower plug 4 is provided with a lower runner 6, one end of the lower runner 6 is communicated with the lower end of the pipe barrel 2, and the other end is selectively communicated with an external fermentation liquid source and a collecting barrel.
At this time, when the probiotic processing device of this embodiment is adopted to carry out the preparation of probiotic, just can accomplish the centrifugation to the probiotic at the bobbin of centrifugation position after, shift the switching with the help of the sleeve with the bobbin between centrifugation position and collection position to can be in the in-process that carries out the collection operation through the probiotic mud of centrifugation absorption at the inner wall to collecting position bobbin, continue to carry out the centrifugation operation to the bobbin of centrifugation position to the probiotic, thereby realize the continuous preparation to the probiotic, improve the preparation efficiency to the probiotic.
As shown in connection with fig. 1 and 2, the probiotic processing device in this embodiment comprises an intermediate sleeve 7 and a spool 8. The middle shaft sleeve 7 is connected with the sleeve 1, the middle shaft sleeve 7 can drive the sleeve 1 to move along the height direction of the pipe barrel 2, and the driving sleeve 1 can rotate relative to the middle shaft sleeve 7. The spool 8 is slidably connected to the intermediate sleeve 7 in the vertical direction through a slider, and can move synchronously with the intermediate sleeve 7 and in the same direction relative to the intermediate sleeve 7. The upper plug 3 and the lower plug 4 are respectively connected with the pipe barrel 2 in a plug-in way, and the upper plug 3 is connected with the spool 8.
At the moment, when the pipe barrel is required to be switched between the centrifugal position and the collecting position, the middle shaft sleeve is controlled to drive the sleeve to move upwards along the height direction of the pipe barrel, so that the pipe barrel can be driven to be separated from the plug-in connection with the lower plug on one hand, and meanwhile, the spool is controlled to move upwards relative to the middle shaft sleeve, so that the spool can drive the upper plug to be separated from the plug-in connection with the pipe barrel, and the quick disconnection between the pipe barrel and the upper plug and the quick disconnection between the pipe barrel and the lower plug are realized; on the contrary, when the pipe barrel is required to be connected with the upper plug and the lower plug, the middle shaft sleeve is controlled to drive the sleeve to move downwards along the height direction of the pipe barrel after the pipe barrel is moved to the centrifugal position, the pipe barrel can be driven to form plug connection with the lower plug, meanwhile, the spool is controlled to move downwards relative to the middle shaft sleeve, and the spool can be used for driving the upper plug to form plug connection with the pipe barrel, so that the quick connection between the pipe barrel and the upper plug and the quick connection between the pipe barrel and the lower plug are realized.
In the probiotic processing device of this embodiment, as shown in fig. 1 and 2, a main support shaft 9 and a replacement telescopic rod 10 are further provided. Wherein the main support shaft 9 is fixedly placed on the ground as a support main structure of the whole processing equipment. The middle shaft sleeve 7 is connected to the main support shaft 9 in a sliding manner along the vertical direction through a sliding block, one end of the telescopic rod 10 is connected with the main support shaft 9, and the other end of the telescopic rod is connected with the middle shaft sleeve 7, so that the middle shaft sleeve 7 is driven to reciprocate linearly relative to the main support shaft 9, and the control tube 2 is connected with the upper plug 3 and the lower plug 4 in a disassembling and assembling manner in a centrifugal position.
Further, as shown in fig. 1, 2, 6 and 7, a rotational connection is adopted between the sleeve 1 and the intermediate sleeve 7, and the main support shaft 9 is sleeved outside the sleeve 1, i.e. the outer surface of the sleeve 1 is directly connected with the main support shaft 9. Meanwhile, a first step groove 11 and a second step groove 12 along the axial direction and a first spiral groove 13 and a second spiral groove 14 are arranged on the outer surface of the sleeve 1, the first step groove 11 and the second step groove 12 are all straight grooves and are symmetrically arranged along the circumferential direction of the sleeve 1, the upper half parts of the first step groove 11 and the second step groove 12 along the axial direction of the sleeve 1 are deep grooves, and the lower half parts of the first step groove 11 and the second step groove 12 are shallow grooves. The two spiral grooves are respectively arranged between the two step grooves, the upper end of the first spiral groove 13 is communicated with the lower end of the deep groove in the first step groove 11, the lower end of the first spiral groove 13 is communicated with the lower end of the shallow groove in the second step groove 12, the upper end of the second spiral groove 14 is communicated with the lower end of the deep groove in the second step groove 12, and the lower end of the second spiral groove 14 is communicated with the lower end of the shallow groove in the first step groove 11. The main support shaft 9 is further provided with a guide pin 15 with a spring, one end of the guide pin 15 is connected with the main support shaft 9, and the other end of the guide pin extends to the first step groove 11, the second step groove 12, the first spiral groove 13 or the second spiral groove 14 and can relatively move along the first step groove 11 and the second step groove 12 and the first spiral groove 13 and the second spiral groove 14.
At this time, in the process of driving the sleeve 1 to move upward relative to the main support shaft 9 by exchanging the telescopic rod 10, when the guide pin 15 moves to the lower end of the deep groove in the first step groove 11 along the upper end of the deep groove in the first step groove 11, the sleeve 1 and the spool 8 just drive the tube 2 to move to the position of disconnecting the upper stopper 3 and the lower stopper 4, then when the sleeve 1 is driven to move upward relative to the main support shaft 9 by exchanging the telescopic rod 10, the guide pin 15 enters the first spiral groove 13 and moves to the lower end of the shallow groove in the second step groove 12 along the first spiral groove 13, thereby forming 180 ° rotation of the sleeve 1 relative to the main support shaft 9, and completing the transfer of the two tubes 2 between the centrifugal position and the collecting position. Then, in the process of replacing the telescopic rod 10 to drive the sleeve 1 to move downwards relative to the main support shaft 9, when the guide pin 15 moves to the upper end position of the deep groove along the second step groove 12, the plug-in connection of the tube 2 at the centrifugal position with the upper plug 3 and the lower plug 4 is completed, and the switching operation of the tube 2 between the centrifugal position and the collecting position is completed.
In this embodiment, utilize the cooperation between change telescopic link, guide pin, step groove and the helicla flute, through the flexible motion of control change telescopic link, can realize the quick, convenient switching operation of tube between centrifugal position and collection position. Of course, in other embodiments, the cartridge may be switched between the off-center position and the collection position in other ways, such as by using a robotic arm instead of a sleeve to control the positional movement of the cartridge.
In addition, in this embodiment two tubes are provided and switched back and forth between a centrifugal position and a collecting position in a 180 ° rotation angle relationship, so that two symmetrically arranged stepped grooves and two spiral grooves between the two stepped grooves are provided on the outer surface of the sleeve. Of course, in other embodiments, according to different usage requirements, a greater number of tubes may be disposed on the sleeve, and the number of the centrifugal positions and the collection positions is correspondingly increased, or the transition positions are disposed between the centrifugal positions and the collection positions, and meanwhile, the number and the arrangement positions of the step grooves and the spiral grooves on the sleeve are correspondingly adjusted, so that the switching operation of the tube positions may be achieved.
As shown in connection with fig. 1 and 3, in the probiotic processing device of the present embodiment, a first rack 16, a second rack 17, and a gear 18 are further included. Wherein the first rack 16 is fixed on the spool 8 in the vertical direction, the second rack 17 is fixed on the main support shaft 9 in the vertical direction, and the gear 18 is rotatably provided on the intermediate shaft sleeve 7, and the gear 18 is simultaneously engaged with the first rack 16 and the second rack 17.
At this time, when the telescopic rod is exchanged to drive the middle shaft sleeve to move up and down relative to the main supporting shaft, the gear moves relatively along the second rack to rotate, and then the gear moves relatively with the first rack to enable the first rack to drive the spool to move up and down relative to the middle shaft sleeve. Therefore, under the cooperation of the telescopic rod, the first rack, the second rack and the gear, the spool can move synchronously along with the middle shaft sleeve and then move relative to the middle shaft sleeve in the same direction, and the dismounting connection of the pipe barrel and the upper and lower plugs is achieved. Of course, in other embodiments, an independent telescopic rod may be further disposed between the spool and the middle shaft sleeve, so as to achieve the effect of controlling the spool to reciprocate relative to the middle shaft sleeve.
In addition, as shown in connection with fig. 1, in the present embodiment, the lower block 4 and the tube 2 are connected in a synchronous rotation by means of a slider in the vertical direction, so that the lower block 4 is connected in a rotation to the main support shaft 9, and the lower flow channel 6 is distributed between the lower block 4 and the main support shaft 9, while a three-way valve 19 is provided on the main support shaft 9. The three-way valve 19 is fixedly connected with the main supporting shaft 9, one valve port is communicated with the lower runner 6, the other valve port is communicated with a fermentation liquid source, and the other valve port is communicated with the collecting barrel. Therefore, the selective communication switching of the lower runner, the fermentation liquid source and the collecting barrel can be rapidly realized by controlling the switching of the on-off relation among the three valve ports in the three-way valve.
In addition, as shown in fig. 1, in this embodiment, the upper plug 3 and the tube 2 are connected in a synchronous rotation manner by a slider along the vertical direction, and the upper plug 3 and the spool 8 are connected in a relative rotation manner, so that the motor 20 for driving the tube 2 to rotate is disposed on the spool 8, and is directly connected with the upper plug 3 by a belt and a pulley, so as to drive the upper plug 3 to rotate relative to the spool 8, and further drive the tube 2 to rotate relative to the sleeve 1 to form a centrifuge. Meanwhile, a waste cavity 21 is further arranged on the spool 8, the waste cavity 21 is sleeved outside the upper plug 3 and is communicated with the upper runner 5, so that waste liquid flowing out through the upper runner 5 when the upper plug 3 rotates synchronously is collected, and then the waste liquid is discharged to an external waste barrel.
Of course, in other embodiments, the tube and the upper plug and the lower plug may be rotationally connected, so that the movable sealing member is used to seal the tube and the upper plug and the lower plug during the plugging connection, and other driving structures are used to directly rotationally drive the tube, such as magnetic driving or worm-and-gear.
As shown in fig. 1, in the fungus processing apparatus of the present embodiment, there are further provided a collecting telescopic rod 22 and a collecting piston 23. The collecting telescopic rod 22 is connected to the main supporting shaft 9 and is located at a collecting position, the collecting piston 23 is located at the protruding end of the collecting telescopic rod 22, and the outer diameter size of the collecting piston 23 corresponds to the inner diameter size of the tube 2.
At this moment, the telescopic rod is collected in the control and stretches out the action, just can drive to collect the piston and pass the bobbin that is located the collection position along vertical direction to carry out quick direct release with the probiotic fungus mud that adsorbs at the inner wall of bobbin, replace the manual work to the clearance of the probiotic fungus mud of the inner wall of bobbin, improve collection efficiency and the convenience to the probiotic fungus mud.
As shown in fig. 1 and 4, the apparatus for processing a raw fungus according to the present embodiment further includes a nozzle 24. The nozzle 24 is located at the lower end of the collecting position middle tube 2 and is capable of injecting high pressure gas to the collecting piston 23.
At this moment, collect telescopic link drive and collect the piston and pass the pipe barrel and accomplish the release to the probiotic bacterial mud after, just can spray high-pressure gas to collecting the piston through the shower nozzle to replace the manual work to collect the surface of piston and clear up the operation, improve the cleaning efficiency and the convenience to collecting the piston.
As shown in connection with fig. 1 and 4, the probiotic processing device of the present embodiment further comprises a collecting chamber 25, an air bag 26 and a control plate 27 constituting a control valve, a control lever 28 and a control spring 29. Wherein the collecting chamber 25 is located at the collecting position for directly receiving the probiotic bacterial sludge pushed out of the tube. The air bag 26 is located on one side of the collection chamber 25 and is provided with an air inlet 30 and an air outlet 31. The spray head 24 is located in the collection chamber 25. The control plate 27 is slidably disposed between the collection chamber 25 and the air bag 26 in the vertical direction, and a first control hole 32, a second control hole 33, and a control groove 34 are provided on the control plate 27. The control spring 29 is connected with the control board 27 to drive the control board 27 to move upwards until the first control hole 32 is in a blocking state, the second control hole 33 is communicated with the collecting cavity 25 and the air inlet 30, the control groove 34 is communicated with the outside atmosphere, wherein an auxiliary air passage 35 is arranged on the main support shaft 9 for communicating the collecting cavity 25 with the second control hole 33, and an auxiliary hole 36 is also arranged for communicating the control groove 34 with the outside atmosphere. One end of the control rod 28 is connected with the control plate 27, the other end extends into the collection cavity 25 and is positioned at the lower end of the tube 2, the collection piston 23 can contact with the control rod 28 after penetrating through the tube 2 and drive the control rod 28 to overcome the acting force of the control spring 29 to drive the control plate 27 to move downwards until the first control hole 32 is communicated with the spray head 24 and the air outlet 31, the second control hole 33 is in a blocking state, and the control groove 34 is communicated with the auxiliary air passage 35 and the auxiliary hole 36, namely, the collection cavity 25 is communicated with the outside atmosphere. Meanwhile, when the tube barrel 2 at the centrifugal position, the upper plug 3 and the lower plug 4 form plug-in sealing connection, the tube barrel 2 at the collecting position synchronously completes plug-in sealing connection with the collecting cavity 25.
At this moment, in the in-process that collecting piston pushed out the probiotic bacterial mud, the volume that collecting chamber and tube constitute diminishes gradually, the gas pressure in the collecting chamber risees, and the control panel makes the air inlet communicate with the collecting chamber through second control hole and auxiliary air flue under control spring's effect, thereby store the high-pressure gas in the collecting chamber to the gasbag, when waiting to collect the piston and stretching into the collecting chamber through the tube, form the contact with the control lever and drive the control panel and overcome the effort of control spring and remove to the position that first control hole and gas outlet communicate, make the high-pressure gas in the gasbag carry to shower nozzle department through gas outlet and first control hole, thereby form the high-pressure jet cleaning to collecting piston, and the unnecessary gas in the collecting chamber then discharges to external atmosphere through auxiliary air flue and control groove, realize the automatic cleaning operation to collecting piston.
In this embodiment, the control formed by the control board, the control rod and the control spring is selected to realize the automatic cleaning operation after the collecting piston moves in place, and of course, in other embodiments, other control valves with structures, such as electromagnetic control valves, can be adopted to actively control the gas storage and cleaning operations.
As shown in connection with fig. 1, the probiotic processing device of this embodiment is further provided with a door panel 37. The door plate 37 is movably connected with the collecting cavity 25, and is used for controlling the opening and closing of the collecting cavity 25 to serve as a channel for collecting the probiotic bacterial sludge.
As shown in fig. 1 to 7, the preparation process of the probiotics by adopting the probiotic processing device of the embodiment specifically comprises the following steps:
step S1, preparing: the upper plug and the lower plug are respectively connected with the upper end and the lower end of the tube barrel of the centrifugal position, the waste material barrel is communicated with the upper end of the tube barrel through an upper runner in the upper plug, and the fermentation liquid source is communicated with the lower end of the tube barrel through a lower runner in the lower plug.
For the specific operation of the probiotic processing device of this embodiment, under the condition that the upper plug 3 and the lower plug 4 are respectively in plug-in sealing connection with the upper end and the lower end of the tube 2 in the centrifugal position, the waste barrel is communicated with the waste cavity 21 through a hose, and two valve ports of the three-way valve 19 are respectively communicated with the fermentation liquid source and the collecting barrel through a hose.
Step S2, feeding and centrifuging: the tube barrel at the centrifugal position is driven to rotate, and meanwhile fermentation liquor of a fermentation liquor source is conveyed into the tube barrel through the lower flow passage and rotates along with the tube barrel, so that the fermentation liquor forms centrifugal separation, wherein probiotic bacterial mud in the fermentation liquor is adsorbed on the inner wall of the tube barrel, and supernatant produced after the fermentation liquor is subjected to centrifugal action flows into the waste material barrel through the upper flow passage.
For the specific operation of the probiotic processing device of this embodiment, the starter motor 20 drives the tube 2 and the lower plug 4 of the centrifugal position to synchronously rotate through the upper plug 3, and meanwhile, the three-way valve 19 is switched to the fermentation liquid source to be communicated with the lower flow channel 6, so that the fermentation liquid is gradually conveyed into the tube 2 through the lower flow channel 6, and the fermentation liquid is centrifugally separated along with the rotation of the tube 2. The probiotic bacterial sludge generated by centrifugal separation of the fermentation liquor is adsorbed on the inner wall of the tube barrel 2, and the supernatant enters the waste cavity 21 through the upper flow passage 5 and is discharged into the waste barrel. Wherein, according to the different use environment, a delivery pump can be arranged between the fermentation liquid source and the three-way valve 19 to improve the delivery pressure of the fermentation liquid, thereby ensuring that the fermentation liquid can be smoothly delivered into the tube barrel 2 for centrifugal separation.
Step S3, exchanging the tube barrel: after the feeding centrifugation in the step S2 is completed, the rotation of the pipe barrel is stopped, the upper blocking and the lower blocking are controlled to be released from being connected with the pipe barrel, the control sleeve moves the pipe barrel with the feeding centrifugation completed to the collecting position and moves the other pipe barrel to the centrifugation position, the upper blocking and the lower blocking are controlled to be connected with the upper end and the lower end of the pipe barrel with the centrifugation position again, and the pipe barrel with the centrifugation position is controlled to carry out feeding centrifugation operation according to the step S2.
For the specific operation of the probiotic processing device in this embodiment, after a large amount of probiotic bacterial sludge is adsorbed on the inner wall of the tube 2 by rotating the tube 2 at the centrifugal position for a period of time, that is, after the staged centrifugal processing of the fermentation broth is completed, the rotation of the motor 20 is stopped, and the three-way valve 19 is controlled to switch to the lower flow channel 6 to be communicated with the collecting barrel, so that the liquid in the tube 2 flows into the collecting barrel through the lower flow channel 6 and the three-way valve 19.
After that, the telescopic rod 10 is controlled to be replaced to extend, so as to drive the middle shaft sleeve 7 to drive the sleeve 1 to move upwards relative to the main support shaft 9, wherein the sleeve 1 directly drives the pipe barrel 2 to move relative to the lower plug 4 to be disconnected with the lower plug, and meanwhile, the spool 8 moves upwards relative to the middle shaft sleeve 7 under the transmission connection of the first rack 16, the second rack 17 and the gear 18, so that the upper plug 3 is driven to move upwards relative to the pipe barrel 2 to be disconnected with the middle sleeve, and in the process, the guide pin 15 connected with the main support shaft 9 just moves relatively to the lower end of the deep groove in the first step groove 11 along the upper end of the deep groove in the first step groove 11. And then, the telescopic rod 10 is controlled to be replaced continuously to extend, at the moment, the guide pin 15 starts to move relatively along the first spiral groove 13, so that the sleeve 1 is driven to rotate relative to the main support shaft 9, and when the guide pin 15 moves relatively to the tail end of the first spiral groove 13 and enters the lower end of the shallow groove in the second step groove 12, the sleeve 1 just rotates 180 degrees, and the transfer of the tube 2 between the centrifugal position and the collecting position is completed.
And then, the telescopic rod 10 is controlled to be exchanged for recovery action, so that the middle shaft sleeve 7 is driven to drive the sleeve 1 to move downwards relative to the main support shaft 9, the sleeve 1 directly drives the pipe barrel 2 to move relative to the lower plug 4 to form plug sealing connection with the sleeve, meanwhile, the spool 8 moves downwards relative to the middle shaft sleeve 7 under the transmission connection of the first rack 16, the second rack 17 and the gear 18, so that the upper plug 3 is driven to move downwards relative to the pipe barrel 2 to form plug sealing connection with the sleeve, and in the process, the pipe barrel 2 at the collecting position moves towards the direction close to the collecting cavity 25 and forms plug sealing connection with the collecting cavity 25, and on the other hand, the guide pin 15 connected with the main support shaft 9 just moves relatively along the second step groove 12 to the upper end position of the deep groove in the second step groove 12.
The exchange operation of the tube between the centrifugal position and the collecting position is completed. And when the exchanging operation is performed again, the exchanging telescopic rod performs telescopic movement again, and the guide pin sequentially moves relative to the deep groove in the second step groove, the second thread groove and the first step groove according to the moving process, so that the exchanging operation is completed.
S4, collecting probiotic bacterial sludge; and (5) carrying out the collection operation of the probiotic bacterial sludge on the pipe barrel positioned at the collection position.
For the specific operation of the probiotic processing device in this embodiment, the collecting telescopic rod 22 is controlled to extend to drive the collecting piston 23 to enter the tube 2 located at the collecting position, so as to push the probiotic bacterial mud located at the inner wall of the tube 2 into the collecting cavity 25, and complete the collecting operation of the probiotic bacterial mud.
Preferably, the preparation process of the probiotics further comprises step S5, cleaning the collecting piston: after the collecting piston passes through the pipe barrel, the spray head is controlled to output high-pressure gas to the collecting piston, so that surface cleaning of the collecting piston is formed. The specific process is as follows:
during the process of extending the collecting telescopic rod 22, the control board 27 is located at the first control hole 32 to be blocked under the action of the control spring 29, the second control hole 33 is communicated with the collecting cavity 25 and the air inlet 30 through the auxiliary air passage 35, the control groove 34 is communicated with the outside atmosphere through the auxiliary hole 36, so that the volume formed by the collecting cavity 25 and the tube 2 is gradually reduced to gradually increase the air pressure in the process of gradually approaching the collecting cavity 25 by the collecting telescopic rod 22 to the collecting piston 23, and then the air is stored into the air bag 26 through the auxiliary air passage 35, the second control hole 33 and the air inlet 30. When the collecting telescopic rod 22 drives the collecting piston 23 to pass through the pipe barrel 2 and extend into the collecting cavity 25, the collecting piston 23 is contacted with the control rod 28, the control rod 28 drives the control plate 27 to move downwards to the first control hole 32 to be communicated with the air outlet 31 against the acting force of the control spring 29, the second control hole 33 is in a blocking state, the control groove 34 moves to be communicated with the auxiliary air passage 35 to communicate the collecting cavity 25 with the outside atmosphere, so that high pressure in the air bag 26 is lifted to be conveyed to the spray head 24 through the air outlet 31 and the first control hole 32, and the spray head 24 outputs high pressure gas to form a cleaning operation on the surface of the collecting piston 23. At the same time, the surplus gas in the collection chamber 25 is discharged to the outside atmosphere through the control groove 34, thereby ensuring a smooth cleaning operation of the collection piston 23.
Thereafter, steps S2-S5 may be repeated for continuous preparation of the probiotic and continuous collection of the probiotic bacterial sludge into the collection chamber 25. And then, the collected probiotic bacterial sludge can be subjected to a blanking operation, namely, the collecting cavity 25 is opened through the door plate 37, and the probiotic bacterial sludge collected in the collecting cavity 25 can be taken out to complete the blanking operation.

Claims (10)

1. The preparation method of the probiotics is characterized in that a probiotic processing device is adopted for carrying out preparation operation of the probiotics, and the probiotic processing device comprises a sleeve, a tube barrel, an upper plug and a lower plug; the sleeve is provided with at least two tubes, and can drive the tubes to switch positions between a centrifugal position and a collecting position, and the tubes positioned in the centrifugal position can rotate; the upper plug and the lower plug are positioned at the centrifugal position and can be detachably connected with the upper end and the lower end of the pipe barrel positioned at the centrifugal position respectively; the upper plug is provided with an upper runner, one end of the upper runner is communicated with the upper end of the pipe barrel, and the other end of the upper runner is communicated with the waste barrel; the lower plug is provided with a lower runner, one end of the lower runner is communicated with the lower end of the pipe barrel, and the other end of the lower runner is selectively communicated with a fermentation liquid source and a collecting barrel;
the preparation method of the probiotics specifically comprises the following steps:
step S1, preparing: the upper plug and the lower plug are respectively connected with the upper end and the lower end of the pipe barrel of the centrifugal position, the waste material barrel is communicated with the upper end of the pipe barrel through an upper flow passage in the upper plug, and a fermentation liquid source is communicated with the lower end of the pipe barrel through a lower flow passage in the lower plug;
step S2, feeding and centrifuging: the tube barrel positioned at the centrifugal position is driven to rotate, meanwhile, fermentation liquor of a fermentation liquor source is conveyed into the tube barrel through the lower flow passage and rotates along with the tube barrel, so that the fermentation liquor is centrifugally separated, probiotic bacterial sludge in the fermentation liquor is adsorbed on the inner wall of the tube barrel, and supernatant produced after the fermentation liquor is subjected to centrifugal action flows into the waste material barrel through the upper flow passage;
step S3, exchanging the tube barrel: stopping the rotation of the pipe barrel after the feeding centrifugation in the step S2 is completed, controlling the upper plug and the lower plug to release the connection with the pipe barrel, controlling the sleeve to move the pipe barrel with the completed feeding centrifugation to a collecting position and move the other pipe barrel to a centrifugation position, controlling the upper plug and the lower plug to be connected with the upper end and the lower end of the pipe barrel in the centrifugation position again, and controlling the pipe barrel in the centrifugation position to carry out feeding centrifugation operation according to the step S2;
s4, collecting probiotic bacterial sludge; and collecting probiotic bacterial sludge from the tube at the collecting position.
2. The method of claim 1, wherein the probiotic processing device comprises an intermediate sleeve and a spool; the middle shaft sleeve is connected with the sleeve, the middle shaft sleeve can drive the sleeve to move along the height direction of the pipe barrel, and the sleeve can rotate relative to the middle shaft sleeve; the spool is in sliding connection with the middle shaft sleeve, and can synchronously move along with the middle shaft sleeve and move relative to the middle shaft sleeve; the lower plug and the upper plug are respectively connected with the pipe barrel in an inserting way, and the upper plug is connected with the spool.
3. The method of claim 2, wherein the probiotic processing device further comprises a main support shaft and a replacement telescopic rod; the middle shaft sleeve is in sliding connection with the main support shaft along the vertical direction, one end of the exchange telescopic rod is connected with the main support shaft, and the other end of the exchange telescopic rod is connected with the middle shaft sleeve so as to drive the middle shaft sleeve to reciprocate linearly relative to the main support shaft.
4. A method of preparing a probiotic according to claim 3, wherein the sleeve is rotatably connected to the intermediate sleeve and the main support shaft is provided outside the sleeve; the outer surface of the sleeve is provided with at least two axial step grooves and at least two spiral grooves, the step grooves are straight grooves and are arranged at intervals along the circumferential direction of the sleeve, the upper half part of each step groove is a deep groove along the axial direction of the sleeve, and the lower half part of each step groove is a shallow groove; one spiral groove is arranged between two adjacent step grooves, the upper end of the spiral groove is communicated with the lower end of a deep groove in one step groove, and the lower end of the spiral groove is communicated with the lower end of a shallow groove in the other step groove; the main support shaft is provided with a guide pin, one end of the guide pin is connected with the main support shaft, and the other end of the guide pin extends out to the step groove or the spiral groove and can relatively move along the step groove and the spiral groove;
in the process that the sleeve moves relative to the main support shaft, when the guide pin moves from the upper end of the deep groove to the lower end of the deep groove, the pipe barrel moves to a position which is disconnected with the upper plug and the lower plug, and when the guide pin moves to the lower end of the shallow groove in the adjacent step groove along the spiral groove, the pipe barrel completes the transfer between the centrifugal position and the collecting position.
5. A method of preparing a probiotic according to claim 3, wherein the probiotic processing device further comprises a first rack, a second rack and a gear; the first rack is fixed on the spool, the second rack is fixed on the main support shaft, the gear is rotatably arranged on the middle shaft sleeve, and the gear is simultaneously meshed with the first rack and the second rack.
6. The method of claim 1, wherein the probiotic processing device further comprises a collection telescoping rod and a collection piston; the collecting telescopic rod is positioned at a collecting position, the collecting piston is positioned at the extending end of the collecting telescopic rod, and the outer diameter size of the collecting piston corresponds to the inner diameter size of the pipe barrel; the collection telescoping rod is capable of driving the collection piston through the tube in a collection position.
7. The method of claim 6, wherein the probiotic processing device further comprises a spray head; the spray head is positioned at the lower end of the tube in the collecting position and can spray high-pressure gas to the collecting piston;
the preparation method of the probiotics further comprises the step S5 of cleaning the collecting piston: after the collecting piston passes through the pipe barrel, the spray head is controlled to output high-pressure gas to the collecting piston, so that surface cleaning of the collecting piston is formed.
8. The method of claim 7, wherein the probiotic processing device further comprises a collection chamber, an air bladder, and a control valve; the collecting cavity is positioned at the collecting position, the air bag is provided with an air inlet and an air outlet, the spray head is positioned in the collecting cavity, and the control valve is positioned between the collecting cavity and the air bag; in the process that the collecting piston moves along the pipe barrel to the direction close to the collecting cavity, the control valve is switched to the collecting cavity to be communicated with the air inlet, and the gas in the collecting cavity is stored to the air bag in a boosting mode; when the collecting piston moves to a position close to the spray head, the control valve is switched to the air outlet to be communicated with the spray head, and high-pressure gas in the air bag is output to the spray head.
9. The method of claim 8, wherein the control valve comprises a control plate, a control rod and a control spring, and the control plate is provided with a first control hole, a second control hole and a control groove; the control panel is arranged between the collecting cavity and the air bag in a sliding manner; the control spring is connected with the control board to drive the control board to move to the state that the first control hole is blocked, the second control hole is communicated with the collecting cavity and the air inlet, and the control groove is communicated with the outside atmosphere; one end of the control rod is connected with the control plate, the other end of the control rod extends into the collecting cavity and is positioned at the lower end of the pipe barrel, the collecting piston penetrates through the pipe barrel and then can contact with the control rod to drive the control rod to overcome the acting force of the control spring so as to drive the control plate to move to the first control hole to be communicated with the spray head and the air outlet, the second control hole is in a blocking state, and the collecting cavity is communicated with the outside atmosphere by the control groove.
10. The method of claim 8, wherein the probiotic processing device further comprises a door panel; the door plate is movably connected with the collecting cavity and used for controlling the opening and closing of the collecting cavity.
CN202210834892.XA 2022-07-16 2022-07-16 Preparation method of probiotics Active CN115011479B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210834892.XA CN115011479B (en) 2022-07-16 2022-07-16 Preparation method of probiotics

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210834892.XA CN115011479B (en) 2022-07-16 2022-07-16 Preparation method of probiotics

Publications (2)

Publication Number Publication Date
CN115011479A CN115011479A (en) 2022-09-06
CN115011479B true CN115011479B (en) 2023-10-20

Family

ID=83082336

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210834892.XA Active CN115011479B (en) 2022-07-16 2022-07-16 Preparation method of probiotics

Country Status (1)

Country Link
CN (1) CN115011479B (en)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102274655A (en) * 2011-08-04 2011-12-14 天津大学 Tower-type filtering device for biomass-based fermented mash
CN203741315U (en) * 2013-12-20 2014-07-30 宁波市镇海海德生化科技有限公司 Collection and separation device for fermentation liquid fungous materials
CN204299549U (en) * 2014-12-10 2015-04-29 陈敬华 The drilling cuttings of drilling wastes dries separator
WO2016044780A1 (en) * 2014-09-19 2016-03-24 Flex Partners Stromal vascular fraction processing devices and methods
CN111279855A (en) * 2018-12-07 2020-06-16 西安九度信息科技有限公司 Seedling growing and planting mechanism unit
CN112321408A (en) * 2020-11-16 2021-02-05 内蒙古金达威药业有限公司 Equipment and method for extracting vitamin K2 from microbial fermentation liquor
JPWO2021054001A1 (en) * 2019-09-17 2021-03-25
CN114146491A (en) * 2021-11-24 2022-03-08 枣庄学院 Filter material belt cleaning device for sewage treatment
CN114561283A (en) * 2022-03-23 2022-05-31 苏州卫生职业技术学院 Full-automatic nucleic acid extraction, constant temperature amplification detect all-in-one
CN115261188A (en) * 2022-09-07 2022-11-01 康菌研生物技术(南京)有限公司 Fermentation process of probiotics

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6869792B2 (en) * 2001-03-16 2005-03-22 Irm, Llc Method and apparatus for performing multiple processing steps on a sample in a single vessel
US7226778B2 (en) * 2003-06-30 2007-06-05 Naturally Recycled Proteins , Llc Apparatus for natural recycling of protein waste

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102274655A (en) * 2011-08-04 2011-12-14 天津大学 Tower-type filtering device for biomass-based fermented mash
CN203741315U (en) * 2013-12-20 2014-07-30 宁波市镇海海德生化科技有限公司 Collection and separation device for fermentation liquid fungous materials
WO2016044780A1 (en) * 2014-09-19 2016-03-24 Flex Partners Stromal vascular fraction processing devices and methods
CN204299549U (en) * 2014-12-10 2015-04-29 陈敬华 The drilling cuttings of drilling wastes dries separator
CN111279855A (en) * 2018-12-07 2020-06-16 西安九度信息科技有限公司 Seedling growing and planting mechanism unit
JPWO2021054001A1 (en) * 2019-09-17 2021-03-25
CN112321408A (en) * 2020-11-16 2021-02-05 内蒙古金达威药业有限公司 Equipment and method for extracting vitamin K2 from microbial fermentation liquor
CN114146491A (en) * 2021-11-24 2022-03-08 枣庄学院 Filter material belt cleaning device for sewage treatment
CN114561283A (en) * 2022-03-23 2022-05-31 苏州卫生职业技术学院 Full-automatic nucleic acid extraction, constant temperature amplification detect all-in-one
CN115261188A (en) * 2022-09-07 2022-11-01 康菌研生物技术(南京)有限公司 Fermentation process of probiotics

Also Published As

Publication number Publication date
CN115011479A (en) 2022-09-06

Similar Documents

Publication Publication Date Title
CN110630885A (en) Automatic filling device for bearing lubricating oil
CN113245324B (en) Tank body oil stain cleaning device for liquefied petroleum gas storage tank
CN115011479B (en) Preparation method of probiotics
CN115350823A (en) Gas backflushing filter material removing device of bag pulling centrifugal machine
CN109731703A (en) The pulling bag of centrifuge stretches pull-up structure and stretches the bag-pulling centrifuge of pull-up structure using this
CN209501992U (en) The pulling bag of centrifuge stretches pull-up structure and stretches the bag-pulling centrifuge of pull-up structure using this
CN116747578A (en) Liquid filtering system and method for biological reaction culture
CN110193792A (en) A kind of hydraulic elastic grip device of phase difference
CN210890991U (en) Automatic filling device for bearing lubricating oil
CN116751712A (en) Preparation method and device of functional probiotics for reducing cholesterol
CN102151677A (en) Three-wash bottle washing machine for wild-mouth bottles
CN108905877A (en) A kind of preparation method of the reactive dye of automation
CN112123777A (en) Three-dimensional printing equipment capable of automatically replacing and cleaning waste materials in spray head
CN114918755A (en) Processing system of stainless steel product
CN110450433B (en) Automatic assembling machine for syringe
CN208185862U (en) A kind of hydraulic pump lubricating arrangement with solenoid directional control valve
CN115487954B (en) Energy-saving indoor intelligent spraying and sterilizing equipment
CN216542632U (en) Coal mine drilling machine clamp disc spring inner sleeve end face machining device
CN220228244U (en) Solenoid valve coupling assembling
CN116899471B (en) Chlorophyll paste dissolving device
CN117548244B (en) Residual filter cake scraping structure applied to centrifugal machine
CN221560599U (en) Double-disc tool magazine of numerical control machine tool
CN118681706A (en) High-tightness sterile separator for biological vaccine engineering
CN115327116B (en) Tumor cell intelligent detector
CN113753432B (en) Intelligent garbage can

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20230922

Address after: Room 518 and 520, building A8, China Cloud valley software park, No.9, Songhua Road, concentrated area, haping Road, economic development zone, Harbin City, Heilongjiang Province

Applicant after: Heilongjiang Baicai Agricultural Technology Co.,Ltd.

Address before: Changjiang Science and Technology Park, No. 40 Nanchang Road, Gulou District, Nanjing City, Jiangsu Province, 210000

Applicant before: Kangjunyan Biotechnology (Nanjing) Co.,Ltd.

GR01 Patent grant
GR01 Patent grant