CN115006608A

CN115006608A - Cerebrovascular coating stent and preparation method thereof

Info

Publication number: CN115006608A
Application number: CN202210084729.6A
Authority: CN
Inventors: 贺迎坤; 段林; 何艳艳; 李天晓
Original assignee: Henan Provincial Peoples Hospital
Current assignee: Henan Provincial Peoples Hospital
Priority date: 2022-01-25
Filing date: 2022-01-25
Publication date: 2022-09-06

Abstract

The invention relates to a cerebrovascular coating stent and a preparation method thereof, and the method comprises the following steps: firstly, cleaning and drying a bracket body; secondly, soaking the stent body in a soaking box filled with a soaking solution for 60 minutes, wherein the soaking solution comprises matrigel, sodium alginate, adhesive protein and VEGF (vascular endothelial growth factor) plasmid, the concentration of the matrigel is 2-5 mg/ml, the concentration of the sodium alginate is 2-4 mg/ml, the concentration of the adhesive protein is 35-45 ug/ml, and the concentration of the VEGF plasmid is 45-55 ng/ul; step three, drying the bracket body soaked in the second step, and soaking the bracket body in a calcium chloride solution for 4-10 minutes; and fourthly, placing the support body soaked in the third step into a vacuum drying oven for drying. The invention provides a cerebrovascular coating stent beneficial to angiogenesis and a preparation method of the cerebrovascular coating stent.

Description

Cerebrovascular coating stent and preparation method thereof

Technical Field

The invention relates to the technical field of auxiliary instruments for treating aneurysm, in particular to a cerebrovascular coating stent and a preparation method thereof.

Background

The cerebrovascular coating stent is also called a blood flow guiding device as an important means for treating aneurysm, and for a parent blood vessel, the problem of vessel rupture easily occurs due to the existence of the aneurysm.

The coating stent comprises a stent body and a functional coating coated on the surface of the stent body, wherein the stent body is usually made of a nickel-titanium alloy material, when the coating stent is sent to an aneurysm position, complications such as inflammatory reaction, thrombosis and the like easily occur, and the functional coating is used for reducing the complications.

However, the functional coating in the prior art has poor functional effect, and after the coating stent is placed in a tumor-carrying blood vessel, the blood vessel is slow to regenerate, which is not beneficial to the treatment and recovery of diseases.

Disclosure of Invention

The invention aims to provide a cerebrovascular coating stent which is beneficial to angiogenesis; the invention also aims to provide a preparation method of the covered cerebrovascular coating stent.

In order to solve the technical problems, the technical scheme of the preparation method of the cerebrovascular coating stent is as follows:

a preparation method of a cerebrovascular coating stent comprises the following steps: firstly, cleaning and drying a bracket body;

secondly, soaking the stent body in a soaking box filled with a soaking solution for 60 minutes, wherein the soaking solution comprises matrigel, sodium alginate, fibronectin and VEGF (vascular endothelial growth factor) plasmids, the concentration of the matrigel is 2-5 mg/ml, the concentration of the sodium alginate is 2-4 mg/ml, the concentration of the fibronectin is 35-45 ug/ml, and the concentration of the VEGF plasmids is 45-55 ng/ul;

step three, drying the bracket body after the soaking in the second step, and soaking in a calcium chloride solution for 4-10 minutes;

and fourthly, placing the support body soaked in the third step into a vacuum drying oven for drying.

The invention has the beneficial effects that: the soaking solution of the invention comprises matrigel, sodium alginate, adhesive protein and VEGF plasmid, after the stent body is soaked in the soaking solution, the surface of the stent body obtains a functional coating containing the matrigel, the sodium alginate, the adhesive protein and the VEGF plasmid, the VEGF has the main function of angiogenesis and can stimulate angiogenesis and angiogenesis, when the blood circulation is insufficient, the VEGF can participate in restoring tissues and an oxygen supply system, the coating of the VEGF on the surface of the stent body is very important for reducing the complication after the stent is implanted, and the application potential of combining the stent with gene therapy is improved by matching the matrigel and the sodium alginate which have strong coating capability and release.

Further, in the first step, the washing to the support body is accomplished through following mode, originally carries out ultrasonic cleaning 10min to the support through acetone, anhydrous alcohol and deionized water, weathers through nitrogen gas to the support body and weathers the realization.

Furthermore, in the second step, the concentration of matrigel is 5mg/ml, the concentration of sodium alginate is 3 mg/ml, the concentration of fibronectin is 40ug/ml, and the concentration of VEGF plasmid is 50 ng/ul.

Further, the VEGF plasmid is a VEGF-A type plasmid.

Furthermore, the vacuum drying oven comprises a drying oven box body, a positioning ball table is arranged at the bottom of the drying oven box body, a plurality of ball table support positioning grooves for supporting the body to be inserted along the radial positioning are evenly distributed on the outer peripheral surface of the positioning ball table, a blocking ball cover capable of moving up and down under the driving of a ball cover driving mechanism is arranged at the top of the drying oven box body, and ball cover support positioning grooves which correspond to the ball table support positioning grooves and are used for inserting the other end of the supporting body are formed in the inner wall of the blocking ball cover.

Furthermore, the ball cover driving mechanism is connected with a blocking ball cover through a ball cover seat, the blocking ball cover is composed of a central ball cover and four edge ball covers sequentially arranged along the circumferential direction of the central ball cover, the edge ball covers are hinged with the central ball cover, the upper end of the central ball cover is fixed on the ball cover seat, and ball cover driving cylinders for driving the edge ball covers to overturn up and down relative to the central ball cover are arranged between the ball cover seat and the edge ball covers.

Furthermore, the diameter of the positioning groove of the spherical cover support is 1.2-1.3 times of the outer diameter of the support body.

Furthermore, the ball cover seat is in running fit with a power output end of the ball cover driving mechanism, a rotating axis of the ball cover seat extends along the vertical direction, the positioning ball table is in running fit with the drying box body, the rotating axis of the positioning ball table and the rotating axis of the ball cover seat are arranged coaxially, a positioning table driving motor for driving the positioning ball table to rotate is arranged at the bottom of the drying box body, at least two transmission rods are fixed on the central ball cover, and a transmission rod slot used for being in positioning insertion fit with the lower end of the corresponding transmission rod to drive the positioning ball table to block synchronous rotation of the ball cover is formed in the positioning ball table.

As a further technical effect of the invention: in order to reduce the size of the vacuum drying oven, more cerebrovascular coating supports can be dried in a small enough space at one time, the invention is provided with a positioning ball table, a plurality of ball table support positioning grooves for radially positioning and inserting the support bodies are uniformly distributed on the positioning ball table, namely, when in use, each support body is respectively positioned and inserted into the corresponding ball table support positioning groove along the radial direction of the positioning ball table, and each support body is radially distributed by taking the spherical shape of the positioning ball table as the center, so that the simultaneous drying of a plurality of cerebrovascular coating supports can be realized.

Furthermore, in order to ensure that each cerebrovascular coating support is uniformly heated and dried in the vacuum drying box, in the invention, the positioning table is driven by the positioning table driving motor to rotate, each cerebrovascular coating support is dried along with the rotation process of the positioning table, and in order to ensure that each cerebrovascular coating support does not fly out of the positioning table under the action of centrifugal force in the rotation process of the positioning table, the blocking ball cover is arranged in the invention, and one end of each cerebrovascular coating support, which is far away from the positioning table, extends into the positioning groove of the corresponding ball cover support and is blocked by the blocking ball cover.

Drawings

The above and other objects, features and advantages of exemplary embodiments of the present disclosure will become readily apparent from the following detailed description read in conjunction with the accompanying drawings. Several embodiments of the present disclosure are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which like reference numerals refer to like or corresponding parts and in which:

FIG. 1 is a process flow diagram of a method for preparing a coated stent for cerebral blood vessels according to the present invention;

FIG. 2 is a schematic view showing the structure of a vacuum drying oven in an embodiment of the production method of the present invention;

FIG. 3 is a view showing a state of use of the vacuum drying oven of FIG. 2;

FIG. 4 is a top view of the blocker ball cover of FIG. 3;

FIG. 5 is a schematic view of the positioning table of FIG. 3 in cooperation with a coated stent for cerebral vessels;

FIG. 6 is a schematic view of the engagement of the cerebrovascular coating support with the table support detent and the dome cover support detent of the present invention;

FIG. 7 is a schematic view of the structure of a soaking tank in the embodiment of the preparation method of the present invention;

FIG. 8 is an enlarged view taken at A in FIG. 7;

FIG. 9 is a schematic view of the positioning post of FIG. 7;

FIG. 10 is a schematic view of the support arm of the lower positioning support of FIG. 9 in an extended position;

FIG. 11 is a schematic view of the support arm of the upper positioning support of FIG. 9 in a deployed position;

FIG. 12 is a schematic view of the positioning post of FIG. 7 engaged with the upper and lower positioning covers;

fig. 13 is an enlarged view at B in fig. 10;

FIG. 14 is an enlarged view at C of FIG. 11;

description of reference numerals: 1. a liquid level; 2. a thrust bearing; 3. mounting holes; 4. an upper positioning cover; 5. a box top plate; 6. a positioning column; 7. a drive shaft; 8. a stirring blade; 9. a soaking box; 10. a box side plate; 11. a box bottom plate; 12. a lower positioning cover; 13. a support leg; 14. a drive sprocket; 15. a drive chain; 16. a servo motor; 17. a motor shaft gear; 18. a positioning cover gear; 19. an outer sleeve; 20. a drive block push rod; 21. jacking the screw tightly; 22. the upper side is positioned and supported; 23. the lower side is positioned and supported; 24. supporting the driving block; 25. a force transfer spring; 26. a driving block limiting structure; 27. a support arm; 28. a cerebrovascular coating stent; 29. an upper positioning cover positioning hole; 30. a lower positioning cover positioning hole; 31. a rotation stopping square hole; 32. a stent body; 33. a vacuum drying oven; 34. a drying box body; 35. a barrier ball cover; 36. a positioning table driving motor; 37. positioning a ball table; 38. a ball cover driving cylinder; 39. a rim ball cover; 40. a central ball cover; 41. a transmission rod; 42. a ball cover support locating slot; 43. a ball cover seat; 44. a ball cage drive mechanism; 45. a table support positioning slot; 46. a transmission rod slot; 47. a ball cover bracket positioning groove.

Detailed Description

In order to facilitate an understanding of the invention, reference will now be made in detail to the present embodiments of the invention, examples of which are illustrated in the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It is to be noted that, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

The embodiment of the preparation method of the cerebrovascular coating stent is shown in figures 1 to 14:

the preparation method comprises the following steps: firstly, cleaning and drying the bracket body 32;

secondly, soaking the bracket body 32 in a soaking box 9 filled with a soaking solution for 60 minutes, wherein the soaking solution comprises matrigel, sodium alginate, adhesive protein and VEGF (vascular endothelial growth factor) plasmids, the concentration of the matrigel is 2-5 mg/ml, the concentration of the sodium alginate is 2-4 mg/ml, the concentration of the adhesive protein is 35-45 ug/ml, and the concentration of the VEGF plasmids is 45-55 ng/ul; the abbreviation of VEGF vascular endothelial growth factor in English is used in the present invention.

Step three, drying the bracket body soaked in the second step, and soaking the bracket body in a calcium chloride solution for 4-10 minutes;

and fourthly, placing the stent body soaked in the third step into a vacuum drying oven 33 for drying. The preparation of the cerebrovascular coating stent is completed.

In the first step, the cleaning of the bracket body 32 is completed in the following mode, the bracket is ultrasonically cleaned for 10min through acetone, absolute ethyl alcohol and deionized water, and the blow-drying of the bracket body is realized through nitrogen blow-drying.

In the second step, the concentration of matrigel is 5mg/ml, the concentration of sodium alginate is 3 mg/ml, the concentration of fibronectin is 40ug/ml, and the concentration of VEGF plasmid is 50 ng/ul.

The VEGF plasmid is a VEGF-A type plasmid.

The vacuum drying oven 33 comprises a drying oven box 34 internally provided with a heating device, a positioning ball table is arranged at the bottom of the drying oven box, a plurality of ball table support positioning grooves 45 used for inserting the support body 32 along the radial positioning are uniformly distributed on the outer spherical surface of the positioning ball table, the diameter of each ball table support positioning groove 45 is matched with the outer diameter of the support body, and the cerebrovascular coating support 28 comprises the support body 32 and a functional coating coated on the periphery of the support body through a second step.

The top of drying cabinet box is provided with the ball cover 35 that blocks that can reciprocate under the drive of ball cover actuating mechanism 44, blocks to be provided with on the inner wall of ball cover 35 in the table of ball constant head tank correspond the setting be used for support body other one end male ball cover support constant head tank 47. In this embodiment, the ball cover driving mechanism 44 is a vertically arranged height adjusting cylinder, a piston rod of the height adjusting cylinder forms a power output end of the ball cover driving mechanism, the diameter of the ball cover support positioning groove is 1.2 times of the outer diameter of the support body, and the ball cover support positioning groove 47 is used for preventing the cerebrovascular coating support from flying out of the positioning table when the positioning table rotates.

The ball cover driving mechanism is connected with a blocking ball cover 35 through a ball cover seat 43, the blocking ball cover 35 is composed of a central ball cover 40 and four edge ball covers 39 sequentially arranged along the circumferential direction of the central ball cover, the edge ball covers 39 are hinged with the central ball cover, the upper end of the central ball cover 40 is fixed on the ball cover seat, and a ball cover driving cylinder 38 for driving each edge ball cover to overturn up and down relative to the central ball cover is arranged between the ball cover seat 43 and each edge ball cover 39.

The ball cover seat 43 is matched with the power output end of the ball cover driving mechanism, namely, a piston rod of the height adjusting cylinder in a rotating mode, the rotating axis of the ball cover seat 43 extends in the vertical direction, the positioning ball table 37 is matched with the bottom of the drying box body in a rotating mode, the rotating axis of the positioning ball table and the rotating axis of the ball cover seat are arranged coaxially, a positioning table driving motor 36 for driving the positioning ball table to rotate is arranged at the bottom of the drying box body, three transmission rods 41 which are arranged at intervals along the circumferential direction are fixed on the central ball cover, and a transmission rod slot 46 which is used for being matched with the lower end of the corresponding transmission rod in a positioning mode to drive the positioning ball table to block the synchronous rotation of the ball cover is arranged on the positioning ball table 37.

In drying the cerebrovascular coating stent 28, the stop ball cage is first moved to a higher position, meanwhile, the edge ball cover is driven by the corresponding ball cover driving cylinder to turn upwards, as shown in figure 2, the bottom of each cerebrovascular coating bracket to be dried is inserted into the corresponding ball table bracket positioning groove on the positioning ball table along the radial direction, then the ball cover is prevented from going downwards, the edge ball cover is turned downwards at the corresponding driving cylinder, the diameter of the positioning groove of the ball cover support is larger than the outer diameter of the support body, can ensure that the edge ball cover turns downwards smoothly, the ball cover bracket positioning groove is smoothly sleeved at one end of the bracket body far away from the positioning ball table, the transmission rod on the central ball cover is also smoothly inserted into the transmission rod slot on the ball positioning sleeve, the positioning table driving motor drives the positioning table to rotate, the transmission rod drives the blocking ball cover and the positioning table to synchronously rotate, and each cerebrovascular coating support is uniformly heated and dried in the rotating process.

The bottom of the soaking tank is provided with supporting legs 13, the soaking tank 9 comprises a tank bottom plate 11, a tank top plate 5 and a tank side plate 10 arranged between the tank top plate 5 and the tank bottom plate 11, the tank top plate 5 and the tank side plate 10 enclose a cavity for filling coating liquid.

Set up four mounting holes 3 along left right direction interval arrangement on the roof board, all be provided with upside location lid in each mounting hole 3, be provided with thrust bearing 4 that the axis extends along upper and lower direction on the roof board corresponding to the below of upside location lid, the lower extreme and the thrust bearing 2 contact cooperation of upside location lid 4. The upper side positioning cover can be taken out of the mounting hole, and the upper side positioning cover can rotate relative to the box top plate through the arrangement of the thrust bearing.

The box bottom plate is provided with a lower side positioning cover 12 which is correspondingly arranged with the upper side positioning cover, the lower side positioning cover 12 is rotatably assembled on the box bottom plate 11, the upper side positioning cover 4 and the lower side positioning cover 12 are coaxially arranged, the lower side positioning cover 12 is provided with a lower side positioning cover positioning hole 30, the upper side positioning cover is provided with an upper side positioning cover positioning hole 29 which is coaxially arranged with the lower side positioning cover positioning hole, and the axes of the lower side positioning cover positioning hole and the upper side positioning cover positioning hole are both extended and arranged along the up-down direction.

The preparation device also comprises positioning columns 6 which are positioned and inserted in the positioning holes of the upper positioning cover and the positioning holes of the lower positioning cover and are used for being inserted in the inner holes of the at least two dense net supports 28 along the vertical sequence, and positioning supports are arranged on the positioning columns 6 corresponding to the bottoms of the dense net supports 28. In the present embodiment, a plurality of positioning posts 6 are arranged at intervals along the circumferential direction between each upper positioning cover and each lower positioning cover.

In this embodiment, the locating support on each locating column has two, the locating support that the position leaned on is called upside locating support 22, the locating support that the position leaned on down is called downside locating support 23, the locating column includes outer sleeve 19, each locating support includes three support arm 27 along outer sleeve circumference interval arrangement, the upper end of support arm 27 links to each other with outer sleeve 19 is articulated, it corresponds the support driving block 24 that sets up with each locating support to be equipped with along the upper and lower direction removal in the outer sleeve 19, be provided with between two adjacent support driving blocks 24 and pass power spring 25, it is the pressure spring to pass power the spring, it is concrete, the upper end and the support driving block lower extreme fixed connection of upside that pass power spring, the lower extreme and the support driving block upper end fixed connection of downside that pass power spring. The length of the force transmission spring is longer than the distance between two adjacent positioning supports.

The upper end of the supporting arm is provided with a stress part extending into the inner side of the outer sleeve, and when the supporting driving block does not apply pressure to the stress part of the supporting arm, the supporting arm 27 is in a retracting position where the lower end turns towards the sleeve under the action of self gravity and the dense net support vertically passes through; when the support driving block applies downward acting force to the stressed part, the support arm 27 is in an expansion position with the lower end turning towards the direction far away from the outer sleeve and used for blocking the dense net support from vertically passing through.

The inner wall of the outer sleeve is provided with a driving block limiting structure 26 corresponding to the lower side of the force bearing part for limiting the downward movement limit of the corresponding supporting driving block, and the driving block limiting structure 26 is an annular limiting disc fixed on the inner wall of the outer sleeve in the embodiment.

The apparatus further comprises a drive block push rod 20 for connection to the uppermost support drive block, in this embodiment the drive block push rod 20 is in guided movable engagement with the upper end of the outer sleeve 19 and the uppermost support drive block is secured to the lower end of the drive block push rod 20. The upper end of the outer sleeve is in threaded connection with a jacking screw 21, the axis of the jacking screw extends along the radial direction of the outer sleeve, the jacking screw 21 is screwed, the jacking screw 21 jacks the driving block push rod 20, and the position of the driving block push rod can be fixed. The upper end of the driving block push rod extends out of the upper end of the upper side positioning cover.

In the process that the driving block push rod pushes down towards the supporting driving block, the lower side positioning support is in contact with the corresponding supporting driving block before the upper side positioning support; in the process of pulling up the driving block by the driving block push rod to support the driving block, the upper side positioning support is separated from the corresponding support driving block before the lower side positioning support.

The bottom of the soaking box is provided with a positioning cover driving mechanism for driving the corresponding lower positioning cover to rotate forwards and reversely, in the embodiment, each lower positioning cover 12 is linearly arranged along the left-right direction, a transmission chain wheel 14 is coaxially fixed on each lower positioning cover 12, the same transmission chain 15 is wound on the transmission chain wheel 14 on each lower positioning cover, and the positioning cover driving mechanism comprises a servo motor 16 in transmission connection with one of the transmission chain wheels. A motor shaft gear 17 is fixed on a motor shaft of the servo motor 16, a positioning cover gear 18 is coaxially fixed on one of the two chain wheels for driving, and the motor shaft gear 17 is meshed with the positioning cover gear 18 for driving.

The coaxial line is fixed with vertical arrangement's transmission shaft 7 on the downside location is covered, offers opening down on the upside location lid 4 with the transmission shaft upper end spline complex spline square hole 31, when needs upside location lid 4 upwards to move away from, spline square hole 31 and transmission shaft break away from, when the upside location lid is placed in upside location lid locating hole, spline square hole and transmission shaft upper end spline the cooperation.

Be provided with stirring vane 8 corresponding with the support number on the transmission shaft, can cluster two dense net supports on every location axle in this embodiment, consequently be provided with two stirring vane who arranges from top to bottom on each transmission shaft.

When the device is used, the dense net supports are required to be sleeved on corresponding positioning columns outside the soaking box 9, the sleeving process is that the driving block push rod is pushed downwards, the driving block push rod drives the upper supporting driving block to move downwards, the force transmission spring is not compressed when the lower supporting driving block does not bear resistance, the upper supporting driving block and the lower supporting driving block synchronously move, the lower supporting driving block presses the stress part at the upper end of the lower positioning supporting arm, the lower positioning supporting arm of the chamber is turned to the unfolding position as shown in figure 4, at the moment, the upper positioning supporting arm is still in the folding position, the first dense net supports can smoothly pass through the upper positioning supporting and are supported on the lower positioning supporting arm, and then the driving block push rod is continuously pushed downwards, because the lower supporting driving block is blocked, the lower supporting driving block can not continuously move downwards, the force transmission spring is compressed, the supporting driving block on the upper side applies pressure to the supporting arm of the positioning support on the upper side, the supporting arm of the positioning support on the upper side is turned up to the unfolding position, then the second dense net support is sleeved on the positioning column, and the dense net support on the upper side is supported by the positioning support on the upper side.

The lower end of a positioning column which is provided with a dense net support in series is inserted into a lower positioning cover, an upper positioning cover is sleeved on the upper end of the positioning column from top to bottom, a rotation stopping square hole at the bottom of the upper positioning cover is sleeved on the upper end of a transmission shaft, and meanwhile, the bottom of the upper positioning cover is supported on a thrust bearing.

The dense net support needs to be soaked in the soaking box for 60 minutes, the first 20 minutes, the servo motor does not act, each dense net support is soaked statically, after 20 minutes of soaking, a certain coating foundation is already arranged on the dense net support, in order to ensure the uniform component and the uniform temperature of the soaking liquid, the servo motor drives each lower side positioning cover to rotate forwards at a constant speed for 10 minutes, then drives each lower side positioning cover to rotate backwards at a constant speed for 10 minutes, the rotating speed of each lower side positioning cover is maintained at 20-30 revolutions per minute, the lower side positioning cover rotates, the transmission shaft is driven to rotate at a constant speed, the stirring blades stir the soaking liquid at a low speed, stir at a low speed, can ensure the uniform component and the uniform temperature of the soaking liquid, ensure the coating effect on the surface of the dense net support, and meanwhile, under the low-speed stirring effect, the liquid resistance is received, the dense net support can incline, one side of the bottom of the dense net support tilts, so that the bottom of the dense net support originally contacted with the support arm is not contacted with the support arm any more, the bottom of the dense mesh support can also be uniformly coated with a coating; and then reversing for 10 minutes to enable the other side of the bottom of the dense mesh support to be tilted so as to realize uniform coating of the coating, finally stopping the servo motor, and standing and soaking the dense mesh support for 20 minutes to finish coating of the coating.

The support arms are unfolded correspondingly through the push rods of the driving blocks in the process that the support frames are sequentially sleeved on the positioning columns, for supporting the bottom of the bracket, for example, for the upper side positioning support and the lower side positioning support, the downward acting force of the push rod of the driving block is transferred to the supporting driving block positioned at the lower part through the force transmission spring, when the supporting driving block at the lower side is not blocked, the force transmission spring is not compressed, the force transmission spring drives the supporting driving block at the lower side to move downwards until the supporting driving block at the lower side is contacted with the stress part of the supporting arm of the positioning support at the lower side to jack the supporting arm at the lower side to the unfolding position, at the moment, because the length of the force transmission spring is longer than the distance between two adjacent positioning supports, the support arm on the upper position rest is still in a retracting position, and the support can smoothly pass through the positioning support on the upper position rest and is supported on the positioning support on the lower position rest.

In the above description of the present specification, the terms "fixed," "mounted," "connected," or "connected," and the like, are to be construed broadly unless otherwise expressly specified or limited. For example, with the term "coupled", it can be fixed, removable, or integral; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship. Therefore, unless the specification explicitly defines otherwise, those skilled in the art can understand the specific meaning of the above terms in the present invention according to specific situations.

From the above description of the present specification, those skilled in the art will also understand the terms used, terms indicating orientation or positional relationship, such as "upper", "lower", "front", "rear", "left", "right", "length", "width", "thickness", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", "central", "longitudinal", "transverse", "clockwise" or "counterclockwise", etc., are based on the orientation or positional relationship as shown in the drawings of the present specification, it is for the purpose of facilitating the explanation of the invention and simplifying the description, and it is not intended to state or imply that the devices or elements involved must be in the particular orientation described, constructed and operated, therefore, the above terms of orientation or positional relationship should not be construed or interpreted as limiting the present invention.

In addition, the terms "first" or "second", etc. used in this specification are used to refer to numbers or ordinal terms for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present specification, "a plurality" means at least two, for example, two, three or more, and the like, unless specifically defined otherwise.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A preparation method of a cerebrovascular coating stent is characterized by comprising the following steps: the method comprises the following steps: firstly, cleaning and drying a bracket body;

2. The method of claim 1, wherein: in the first step, the washing to the support body is accomplished through following mode, originally carries out ultrasonic cleaning 10min to the support through acetone, anhydrous alcohol and deionized water, weathers through nitrogen gas to the weathering realization of support body.

3. The method of claim 1, wherein: in the second step, the concentration of matrigel is 5mg/ml, the concentration of sodium alginate is 3 mg/ml, the concentration of fibronectin is 40ug/ml, and the concentration of VEGF plasmid is 50 ng/ul.

4. The method of claim 1, wherein: the VEGF plasmid is a VEGF-A type plasmid.

5. The production method according to any one of claims 1 to 4, characterized in that: the vacuum drying box comprises a drying box body, the bottom of the drying box body is provided with a positioning ball table, and the outer peripheral surface of the positioning ball table is uniformly provided with a plurality of ball table support positioning grooves for the support body to be inserted along the radial positioning.

6. The method of claim 5, wherein: the top of drying cabinet box is provided with the ball cover that blocks that can reciprocate under the drive of ball cover actuating mechanism, blocks to be equipped with on the inner wall of ball cover and is used for the inserted ball cover support constant head tank of the other one end of support body that corresponds the setting with ball platform support constant head tank.

7. The method of claim 6, wherein: the ball cover driving mechanism is connected with the blocking ball cover through the ball cover seat, the blocking ball cover is composed of a central ball cover and four edge ball covers sequentially arranged along the circumferential direction of the central ball cover, the edge ball covers are hinged with the central ball cover, the upper end of the central ball cover is fixed on the ball cover seat, and a ball cover driving cylinder for driving each edge ball cover to overturn up and down relative to the central ball cover is arranged between the ball cover seat and each edge ball cover.

8. The method of claim 7, wherein: the diameter of the spherical cover support positioning groove is 1.2-1.3 times of the outer diameter of the support.

9. The method of claim 7, wherein: the ball cover seat is in running fit with a power output end of the ball cover driving mechanism, a rotating axis of the ball cover seat extends in the vertical direction, the positioning ball table is in running fit with the drying box body, the rotating axis of the positioning ball table and the rotating axis of the ball cover seat are arranged coaxially, a positioning table driving motor for driving the positioning ball table to rotate is arranged at the bottom of the drying box body, at least two transmission rods are fixed on the central ball cover, and a transmission rod slot used for being in positioning insertion fit with the lower end of the corresponding transmission rod to drive the positioning ball table to block synchronous rotation of the ball cover is formed in the positioning ball table.

10. Cerebrovascular coats the support, its characterized in that: the cerebrovascular coating stent is prepared by the preparation method of any one of claims 1 to 9.