CN114993765B - Large-scale zooplankton environment DNA field sample collection system - Google Patents
Large-scale zooplankton environment DNA field sample collection system Download PDFInfo
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- CN114993765B CN114993765B CN202210671387.8A CN202210671387A CN114993765B CN 114993765 B CN114993765 B CN 114993765B CN 202210671387 A CN202210671387 A CN 202210671387A CN 114993765 B CN114993765 B CN 114993765B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
A large-scale zooplankton environment DNA field sample collection device relates to a sample collection device. The invention aims to solve the problems of low acquisition efficiency and waste of physical strength of acquisition personnel in the existing large-scale zooplankton environment DNA field sample acquisition. A sample bottle (1) is vertically arranged on the ground, a container (3), a quantitative container (4) and a vacuumizing connecting piece (5) are sequentially connected from bottom to top and are vertically arranged right above the sample bottle (1), a conical net (2) is detachably arranged on the lower end face of the container (3), a vacuum pump (6) is connected with the vacuumizing connecting piece (5), and a collecting pipe assembly is arranged on the vacuumizing connecting piece (5); one end of a telescopic hard tube (7) of the collecting tube assembly is detachably connected with the vacuumizing connecting piece (5), and the other end of the telescopic hard tube (7) is used for wading collection or is not waded collection after being connected with a telescopic soft tube (8). The invention is used for collecting DNA field samples of large zooplankton environment.
Description
Technical Field
The invention relates to a sample collecting device, in particular to a large-scale zooplankton environment DNA field sample collecting device.
Background
At present, the traditional water sampler (commonly used 5L) is used for collecting water samples in the field of environmental DNA sample collection, and then a plankton net is used for filtering the water samples so as to collect samples. The acquisition method is more suitable for acquiring traditional resource samples. However, the environmental DNA sample collection amount is several times of the traditional resource sample collection amount, and the field manual operation time can also be several times, so that the daily field sample collection amount is reduced, meanwhile, the manual collection wastes physical strength and is not scientific, and the tool and the collection method are not beneficial to carrying out the field collection work of the environmental DNA sample.
In conclusion, the problems of low collection efficiency and waste of physical strength of collection personnel exist in the existing large-scale zooplankton environment DNA field sample collection.
Disclosure of Invention
The invention aims to solve the problems of low acquisition efficiency and waste of physical strength of acquisition personnel in the existing large-scale zooplankton environment DNA field sample acquisition. Further provides a large zooplankton environment DNA field sample collecting device.
The technical scheme of the invention is as follows: a large-scale zooplankton environment DNA field sample collection device comprises a sample bottle, a conical net, a container, a quantitative container, a vacuumizing connecting piece, a vacuum pump and a collection pipe assembly, wherein the sample bottle is vertically installed on the ground, the container, the quantitative container and the vacuumizing connecting piece are sequentially connected from bottom to top and are vertically installed right above the sample bottle, the conical net is detachably installed on the lower end face of the container, the vacuum pump is connected with the vacuumizing connecting piece, and the collection pipe assembly is installed on the vacuumizing connecting piece; the collection pipe assembly comprises a telescopic hard pipe and a soft pipe, one end of the telescopic hard pipe is detachably connected with the vacuumizing connecting piece, and the other end of the telescopic hard pipe is waded with water to be collected or is not waded with water to be collected after being connected with the soft pipe.
Furthermore, flexible hard pipe includes first elbow, telescopic link and second elbow, and the one end and the evacuation connecting piece of first elbow are connected, and the other end and the one end of telescopic link of first elbow are connected, and the other end and the second elbow of telescopic link are connected, and the second elbow is scalable with the side of being connected of soft pipe.
Further, it also includes the counter weight subassembly, and the terminal installation of counter weight subassembly is at the hose.
Further, the counter weight subassembly includes that the platform absorbs water the cavity, the transition absorbs water the chamber, a plurality of counter weight units and the pipe that absorbs water of absorbing water of a plurality of counter weight units install on the lower terminal surface of the cavity that absorbs water of platform and with the platform chamber intercommunication that absorbs water, the transition absorbs water the chamber install on the up end of the cavity that absorbs water of platform and with the platform chamber intercommunication that absorbs water, a plurality of levels absorb water tub circumference and horizontal installation on the lateral wall in the transition chamber that absorbs water.
Further, each water absorption weight unit and each horizontal suction pipe A-3 are provided with valves.
Furthermore, every counter weight unit that absorbs water all includes the counter weight barrel, supports buffer spring, base and vertical pipe that absorbs water, and the base is embedded and clamps in the lower part of counter weight barrel, and the lower extreme that supports buffer spring supports and establishes on the base, and the upper end that supports buffer spring is connected and supports to establish on the lower terminal surface of platform chamber that absorbs water along with counter weight barrel and platform chamber that absorbs water's lower terminal surface, and vertical pipe that absorbs water wears to establish and stretches out the counter weight barrel in supporting buffer spring.
Further, the end of the horizontal suction pipe is in a horn shape.
Compared with the prior art, the invention has the following effects:
1. the invention can be suitable for different sampling requirements of depths of lakes, rivers, streams, reservoirs and the like, and is realized by the following technical means:
the telescopic hard tube and the telescopic soft tube with the length set according to the actual situation are adopted, and when sampling is required in a lake, generally 2m, 3m or 5m, the flexible hard tube is provided with the soft tube; when the sampling device is used in streams, the sampling depth of 0.5m can be only required to be set at the telescopic hard tube; no matter under which kind of sampling environment, the homoenergetic is suitable for, especially under the effect of vacuum pump 6, with sample collection fast, need not the workman and gather for a long time, has improved the efficiency of DNA sampling, also the effectual intensity of labour who alleviates workman's sampling.
2. The balance weight component A not only can play a role of balancing the end part of the collecting pipe component (the balance weight component A is suitable for lakes, and the collected water sample is the water sample in the middle of the water layer of the lakes), but also can absorb water at the bottom, so that the water absorption area is increased, and the water absorption efficiency is further improved.
In addition, according to the invention, when the whole collecting pipe assembly needs to be supported, a plurality of water absorption counterweight units B are arranged at the bottom of the water body to support the end part of the whole collecting pipe assembly (the condition is suitable for wading situations such as creeks and the like), and in the water absorption process, a plurality of horizontal water absorption pipes A-3 which are horizontally arranged are used for increasing the water absorption area, so that silt can not be absorbed on the premise of ensuring the support, and the sampling quality is further ensured.
Drawings
FIG. 1 is a schematic view of the present invention with the weight assembly A removed; FIG. 2 is a schematic structural diagram of the lake sampling device according to the present invention; fig. 3 is an enlarged view of the weight assembly a.
Detailed Description
The first embodiment is as follows: the embodiment is described by combining fig. 1 to fig. 2, and comprises a sample bottle 1, a conical net 2, a container 3, a quantitative container 4, a vacuumizing connecting piece 5, a vacuum pump 6 and a collecting pipe assembly, wherein the sample bottle 1 is vertically arranged on the ground, the container 3, the quantitative container 4 and the vacuumizing connecting piece 5 are sequentially connected from bottom to top and vertically arranged right above the sample bottle 1, the conical net 2 is detachably arranged on the lower end face of the container 3, the vacuum pump 6 is connected with the vacuumizing connecting piece 5, and the collecting pipe assembly is arranged on the vacuumizing connecting piece 5; the collection pipe assembly comprises a telescopic hard pipe 7 and a soft pipe 8, one end of the telescopic hard pipe 7 is detachably connected with the vacuumizing connecting piece 5, and the other end of the telescopic hard pipe 7 is waded with water to be collected or is connected with the soft pipe 8 to be collected in a non-wading mode.
The container 3 of the invention is a conical small-volume container, generally 30-60ml, the conical net 2 is clamped at the bottom of the conical small-volume container and is detachably clamped, and large planktons enter the sample bottle 1 after passing through the conical net 2.
This embodiment adopts ration container 4, even when sampling at a plurality of different sampling points, is convenient for carry out the experiment contrast. Meanwhile, the problem that no way for quantifying is available when the existing water pump pumps water can be solved. Even if the prior art adopts a flowmeter and a flow valve to carry out quantitative measurement, the problem of high cost still exists. Therefore, the quantitative container adopted by the invention not only can realize the quantitative, but also has low cost and high extraction efficiency.
The second embodiment is as follows: the embodiment is described with reference to fig. 1 and 2, the telescopic hard tube 7 of the embodiment comprises a first elbow 7-1, a telescopic rod 7-2 and a second elbow 7-3, one end of the first elbow 7-1 is connected with the vacuumizing connecting piece 5, the other end of the first elbow 7-1 is connected with one end of the telescopic rod 7-2, the other end of the telescopic rod 7-2 is connected with the second elbow 7-3, and the connecting side of the second elbow 7-3 and the soft tube 8 is telescopic. So set up, this embodiment has adopted two elbows, can satisfy the collection needs of various types of water. Other components and connections are the same as in the first embodiment.
In practical use, the telescopic rod 7-2 is mainly used for adjusting the distance of sampling, and the connecting side of the second elbow 7-3 and the soft tube 8 is telescopic for adjusting the depth of sampling water.
The third concrete implementation mode: the present embodiment is described with reference to fig. 2 and 3, and further includes a weight member a, which is mounted on the end of the flexible tube 8. According to the arrangement, the counterweight is particularly suitable for counterweight in a water depth environment, and in the actual use process, the counterweight component A can play a role in counterweight, supporting and increasing the water absorption area. Other components and connection relationships are the same as those in the second embodiment.
The fourth concrete implementation mode: the embodiment is described with reference to fig. 3, the counterweight assembly a of the embodiment comprises a platform water-absorbing chamber a-1, a transition water-absorbing chamber a-2, a plurality of water-absorbing counterweight units B and a plurality of horizontal water-absorbing pipes a-3, wherein the plurality of water-absorbing counterweight units B are installed on the lower end face of the platform water-absorbing chamber a-1 and are communicated with the platform water-absorbing chamber a-1, the transition water-absorbing chamber a-2 is installed on the upper end face of the platform water-absorbing chamber a-1 and is communicated with the platform water-absorbing chamber a-1, and the plurality of horizontal water-absorbing pipes a-3 are circumferentially and horizontally installed on the outer side wall of the transition water-absorbing chamber a-2. So set up, when the area that absorbs water of increase, be convenient for realize the counter weight. Other compositions and connection relations are the same as those of the third embodiment.
The fifth concrete implementation mode is as follows: referring to fig. 3, the present embodiment will be described, in which each of the water absorption weight units B and each of the horizontal suction pipes a-3 is provided with a valve a-4. By the arrangement, water can be conveniently absorbed at the water absorption weight unit B or the horizontal water absorption pipe A-3 according to actual conditions, and the use is more flexible. Other compositions and connection relations are the same as those of any one of the first to fourth embodiments.
The sixth specific implementation mode: the embodiment is described with reference to fig. 1 to 3, each water-absorbing counterweight unit B of the embodiment comprises a counterweight cylinder B-1, a supporting buffer spring B-2, a base B-3 and a vertical water-absorbing pipe B-4, wherein the base B-3 is embedded and clamped at the lower part of the counterweight cylinder B-1, the lower end of the supporting buffer spring B-2 is abutted against the base B-3, the upper end of the supporting buffer spring B-2 is abutted against the lower end face of the platform water-absorbing chamber A-1 along with the connection of the counterweight cylinder B-1 and the lower end face of the platform water-absorbing chamber A-1, and the vertical water-absorbing pipe B-4 penetrates through the supporting buffer spring B-2 and extends out of the counterweight cylinder B-1. By the arrangement, a water absorption space is provided for the vertical water absorption pipe B-4 while balancing weight, and the water absorption area of the hose is increased. Other compositions and connection relations are the same as those of any one of the first to the fifth embodiments.
The seventh embodiment: referring to fig. 3, the horizontal suction pipe a-3 of the present embodiment is described as having a flared end. So set up, tubaeform suction tube can effectively increase the area of absorbing water. Other compositions and connection relationships are the same as any one of the first to sixth embodiments.
The depth water detection front end can be freely arranged by adjusting the telescopic hard tube 7 and the soft tube 8; the vacuum pump provides water absorption power; the sample bottle is used for collecting samples. The invention mainly solves the problem that the method can be suitable for different sampling requirements of depths of lakes, rivers, streams, reservoirs and the like; and (4) collecting samples of different substrate types such as sand bottom, mud bottom, stone bottom and the like.
The working principle of the invention is as follows:
and connecting the devices before collection. The telescopic rod is adjusted to a proper position according to the specific sampling requirement of the collected water body, and if the water sample is collected on a ship or collected under ice, the water sample is connected with the hose with the balance weight to a proper position. The middle-end folding container is unfolded to meet the quantitative requirement, the water flow can enter the folding container by opening a vacuum pump switch, when the container is full, a relief valve is opened to allow 5L of water to flow into the conical net, and the sample is collected from the plankton net to the sample bottle when the water quantity to be collected meets the requirement.
In practical use, the valve A-4 of the whole water absorption weight unit B can be closed, and in any case, the horizontal suction pipe A-3 is adopted for sampling. Thereby increasing the water absorption area.
Claims (3)
1. The utility model provides a field sample collection system of large-scale zooplankton environment DNA, it includes sample bottle (1), its characterized in that: the quantitative vacuum collection device is characterized by further comprising a counterweight assembly (A), a conical net (2), a container (3), a quantitative container (4), a vacuumizing connecting piece (5), a vacuum pump (6) and a collection pipe assembly, wherein the sample bottle (1) is vertically arranged on the ground, the container (3), the quantitative container (4) and the vacuumizing connecting piece (5) are sequentially connected from bottom to top and are vertically arranged right above the sample bottle (1), the conical net (2) is detachably arranged on the lower end face of the container (3), the vacuum pump (6) is connected with the vacuumizing connecting piece (5), and the collection pipe assembly is arranged on the vacuumizing connecting piece (5);
the collecting pipe assembly comprises a telescopic hard pipe (7) and a soft pipe (8), one end of the telescopic hard pipe (7) is detachably connected with the vacuumizing connecting piece (5), and the other end of the telescopic hard pipe (7) is used for wading collection or is not waded collection after being connected with the soft pipe (8);
the balance weight component (A) is arranged at the tail end of the flexible pipe (8) and comprises a platform water absorption cavity (A-1), a transition water absorption cavity (A-2), a plurality of water absorption balance weight units (B) and a plurality of horizontal water absorption pipes (A-3), the plurality of water absorption balance weight units (B) are arranged on the lower end face of the platform water absorption cavity (A-1) and communicated with the platform water absorption cavity (A-1), the transition water absorption cavity (A-2) is arranged on the upper end face of the platform water absorption cavity (A-1) and communicated with the platform water absorption cavity (A-1), and the plurality of horizontal water absorption pipes (A-3) are circumferentially and horizontally arranged on the outer side wall of the transition water absorption cavity (A-2);
each water absorption weight unit (B) and each horizontal water absorption pipe (A-3) are provided with a valve (A-4);
each water absorption counterweight unit (B) comprises a counterweight cylinder (B-1), a supporting buffer spring (B-2), a base (B-3) and a vertical water absorption pipe (B-4), wherein the base (B-3) is embedded and clamped at the lower part of the counterweight cylinder (B-1), the lower end of the supporting buffer spring (B-2) is abutted against the base (B-3), the upper end of the supporting buffer spring (B-2) is abutted against the lower end face of the platform water absorption chamber (A-1) along with the connection of the counterweight cylinder (B-1) and the lower end face of the platform water absorption chamber (A-1), and the vertical water absorption pipe (B-4) penetrates through the supporting buffer spring (B-2) and extends out of the counterweight cylinder (B-1).
2. The large zooplankton environment DNA field sample collection device of claim 1, characterized in that: the telescopic hard pipe (7) comprises a first elbow (7-1), a telescopic rod (7-2) and a second elbow (7-3), one end of the first elbow (7-1) is connected with the vacuumizing connecting piece (5), the other end of the first elbow (7-1) is connected with one end of the telescopic rod (7-2), the other end of the telescopic rod (7-2) is connected with the second elbow (7-3), and the connecting side of the second elbow (7-3) and the soft pipe (8) is telescopic.
3. The large zooplankton environment DNA field sample collection device of claim 2, characterized in that: the end part of the horizontal suction pipe (A-3) is in a horn shape.
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| CN202210671387.8A CN114993765B (en) | 2022-06-14 | 2022-06-14 | Large-scale zooplankton environment DNA field sample collection system |
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| CN202210671387.8A CN114993765B (en) | 2022-06-14 | 2022-06-14 | Large-scale zooplankton environment DNA field sample collection system |
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| CN114993765B true CN114993765B (en) | 2022-11-29 |
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