CN114989546A - FEP film for producing cell therapy liquid storage bag - Google Patents
FEP film for producing cell therapy liquid storage bag Download PDFInfo
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Abstract
The invention discloses an FEP film for producing a cell therapy liquid storage bag, which relates to the technical field of composite materials and is a composite film material at least comprising FEP resin and modified polyimide; the modified polyimide comprises products of polyimide activation reaction and amidation reaction; the raw materials for the activation reaction comprise spermine or polyethyleneimine; the amidation reaction raw material comprises perfluorooctanoic acid; the polyimide is a transparent polyimide material. The FEP film provided by the invention has excellent light transmission and oxygen permeability, and meanwhile, the mechanical property of the FEP film is obviously improved, and the tensile strength is obviously enhanced; and the friction and wear resistance of the membrane material is effectively improved, the application field of the membrane material is expanded, and the membrane material has huge application prospect particularly in the production field of cell therapy liquid storage bags.
Description
Technical Field
The invention belongs to the technical field of composite materials, and particularly relates to an FEP film for producing a cell therapy liquid storage bag.
Background
Cell therapy is a method of disease treatment using autologous or allogeneic adult cells or stem cells. Generally, healthy cells or stem cells are transplanted into a patient, and the diseased cells are supplemented and replaced to activate organ functions, improve organ activities, improve organ quality, delay organ diseases and restore normal physiological functions of organs, so that the purpose of treating diseases or resisting aging is achieved. Cell therapy has been applied to delay aging, build up health, etc. in the field of health maintenance. In the process of cell therapy, cell fluid cultured outside needs to be input into a medical target body, and higher requirements are put forward on a liquid storage bag for bearing the cell fluid.
FEP material (perfluoroethylene propylene copolymer) is not cytotoxic and tissue toxic because it does not contain plasticizers and the like that may infiltrate and alter fragile cellular tissues, while also being suitable for storage of antibodies, cytokines and other genetic materials; the cell culture medium has good air permeability and can transmit high-degree oxygen to cells in culture; the water permeability is extremely low, the water loss can be ignored and a wet incubator does not need to be provided for the water loss; the light transmittance is higher than 95%, and the light can be directly observed through a microscope, so that the risk of polluting cells and tissues is avoided; and is also suitable for low-temperature storage. Therefore, the FEP material has wide application prospect in the field of cell therapy liquid storage bag production. However, while the FEP material has a series of excellent properties, its weak points are also obvious, especially its mechanical properties are poor and it is easy to break, which seriously affects its application.
Disclosure of Invention
The invention aims to provide an FEP film for producing a cell therapy liquid storage bag, which has excellent light transmission and oxygen permeability, obviously improved mechanical properties and obviously enhanced tensile strength; and the friction and wear resistance of the film material is effectively improved, and the application field of the film material is expanded.
The technical scheme adopted by the invention for realizing the purpose is as follows:
an FEP film which is a composite film material comprising at least an FEP resin, and,
modified polyimide; the modified polyimide comprises products of polyimide activation reaction and amidation reaction; the raw materials for the activation reaction comprise spermine or polyethyleneimine; the amidation reaction raw material comprises perfluorooctanoic acid;
the polyimide is a transparent polyimide material. The FEP film provided by the invention is a composite film material, polyimide and FEP resin are well compounded through chemical design to obtain the film material with excellent comprehensive performance, and the FEP film can be better applied to the production of cell therapy liquid storage bags. In the invention, spermine is adopted to perform ring opening activation on polyimide, and then a compound containing a carboxyl functional group is used for chemical modification, so that the polyimide can be well compounded with FEP resin; the modified polyimide has more excellent dispersing ability in an FEP resin matrix, the modified polyimide and the FEP resin are mixed more uniformly, the mechanical property of the composite film material is obviously improved, and the tensile strength of the composite film material is obviously enhanced; the formed membrane structure is more uniform, the network stability is higher, and the composite membrane material has better friction and wear resistance; meanwhile, the oxygen permeability of the membrane material is further improved, the high delivery of oxygen is better realized, and the requirement on oxygen in the cell culture process is met. Although the visible light transmittance of the FEP film prepared by the invention is reduced, the visible light transmittance is still maintained at a higher light transmission capability level, the visible light transmittance is more than 91 percent, and the observation of morphological characteristics and the like in the cell treatment process can be met.
In a specific embodiment, the mass ratio of the modified polyimide to the FEP resin is 0.01-0.02: 1.
in a particular embodiment, the tensile strength of the FEP film is > 24 MPa; more preferably, the tensile strength of the FEP film is > 27 MPa.
The preparation method of the FEP film comprises the following steps:
activating polyimide, namely impregnating a polyimide material by adopting spermine or a polyethylene imine aqueous solution, and performing an activation reaction to obtain activated polyimide;
modifying polyimide, namely chemically modifying the activated polyimide by adopting perfluorooctanoic acid through amidation reaction to obtain modified polyimide;
preparing the FEP composite film, namely compounding the modified polyimide with the FEP resin, and preparing the FEP film by adopting a tape casting method.
Further, the preparation method of the FEP film comprises the following specific steps:
activating polyimide, namely cutting a CPI film, soaking the cut CPI film into a spermine or polyethylene imine water solution for 5-8 hours or 20-24 hours, washing the CPI film with deionized water for multiple times, and drying the CPI film at 50-60 ℃ for 10-12 hours to obtain activated polyimide;
modifying polyimide, namely adding activated polyimide into a methanol solution of perfluorooctanoic acid, stirring and reacting for 20-24 hours, washing for 3-5 times with methanol, washing for multiple times with deionized water, and drying for 10-12 hours at 50-60 ℃ to obtain modified polyimide;
preparing the FEP composite film, namely compounding the modified polyimide with the FEP resin, and preparing the FEP film by adopting an extrusion casting method.
In a specific embodiment, the concentration of the spermine or polyethyleneimine aqueous solution is 9-12 wt%.
In one embodiment, the concentration of the methanol solution of perfluorooctanoic acid is 4 to 7 wt%.
In a specific embodiment, the mass ratio of the activated polyimide to the perfluorooctanoic acid is 1: 0.2 to 0.4.
It should be noted that the process steps of the extrusion casting method are prior art, and can be conventionally understood by those skilled in the art.
In a specific embodiment, the FEP film thickness is 0.1-0.3 mm.
More preferably, in the FEP film production method, 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid is used instead of perfluorooctanoic acid. According to the invention, in the polyimide modification process, 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid is grafted on the activated polyimide through amidation reaction, so that the mixing uniformity of the modified polyimide and FEP resin is further enhanced, the integral network structure of the composite material is beneficially influenced, the mechanical property of the membrane material is remarkably improved, and the tensile strength is further enhanced; the water vapor permeability of the membrane material is obviously reduced, and the loss of water is reduced; under the condition that polyimide is activated by polyethylene imine, the formed film structure is more stable, and the friction and wear resistance is obviously enhanced; meanwhile, the two are compounded in a synergistic manner, so that the oxygen permeability of the membrane material is improved better, and the level of pure FEP resin membrane is basically achieved.
The invention also aims to provide the application of the modified polyimide in enhancing the mechanical property of the FEP film.
The invention also discloses application of the FEP film in preparation of a liquid storage bag for cell therapy.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the polyimide is modified by adopting compounds such as spermine, so that the purpose of more uniform compounding of the polyimide and FEP resin is realized, the mechanical property of the composite film material is obviously improved, and the tensile strength is obviously enhanced; the composite film material has better friction and wear resistance; meanwhile, the oxygen permeability of the membrane material is further improved, and the high delivery of oxygen is better realized. In addition, the activated polyimide is further modified by adopting 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid, so that the mechanical property of the membrane material can be obviously improved, and the tensile strength is further enhanced; the water vapor permeability of the membrane material is reduced, and the loss of water is reduced; meanwhile, under the condition that polyimide is activated by polyethyleneimine, the friction and wear resistance of the membrane material is improved more obviously; and the effect of improving the oxygen permeability of the membrane material is better. Compared with a pure FEP film, the FEP film prepared by the invention has the advantages that although the oxygen permeability, the water vapor permeability and the visible light transmittance are reduced, the FEP film still maintains a higher light transmittance level, and meets the basic requirements of a cell treatment process on a liquid storage bag; on the basis, the mechanical property of the film material is greatly improved, the processing property is more excellent, and the application field is expanded.
Therefore, the FEP film for producing the cell therapy liquid storage bag has excellent light transmission and oxygen permeability, the mechanical property of the FEP film is obviously improved, and the tensile strength is obviously enhanced; and the friction and wear resistance of the membrane material is effectively improved, and the application field of the membrane material is expanded.
Drawings
FIG. 1 shows IR spectra of a polyimide, an activated polyimide and a modified polyimide in test example 1 of the present invention.
Detailed Description
The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:
the CPI film used in the embodiment of the invention is purchased from Huizhou Engineer high-tech New Material Co; the FEP resin used was purchased from Itelli plastics materials, Inc., Suzhou.
Example 1:
preparation of FEP film:
polyimide activation, namely cutting a CPI film, soaking the cut CPI film into a 10.5 wt% concentration spermine aqueous solution, soaking for 7 hours, washing with deionized water for multiple times, and drying at 60 ℃ for 12 hours to obtain activated polyimide;
modifying polyimide, namely adding a 5.6 wt% methanol solution of perfluorooctanoic acid into activated polyimide, stirring for reaction for 22 hours, washing with methanol for 4 times, washing with deionized water for multiple times, and drying at 60 ℃ for 12 hours to obtain modified polyimide; specifically, the mass ratio of the activated polyimide to the perfluorooctanoic acid is 1: 0.32;
the FEP composite film is prepared by compounding modified polyimide and FEP resin (the mass ratio of the modified polyimide to the FEP resin is 0.014: 1) and preparing the FEP film with the thickness of 0.18mm by adopting an extrusion casting method. Wherein, the extrusion casting method comprises the following steps: extruding by an extruder, casting by a T-shaped port die, cooling by an air knife and a cooling roller, carrying out corona treatment, cutting waste edges and coiling; the extrusion process of the extruder has the melting temperature of 280 ℃ and the extrusion temperature of 310 ℃.
Example 2:
the FEP film was produced as in example 1 except that:
the concentration of the spermine aqueous solution is 9.4 wt%;
the concentration of the methanol solution of perfluorooctanoic acid was 4.6 wt%;
the mass ratio of the activated polyimide to the perfluorooctanoic acid is 1: 0.23;
the mass ratio of the modified polyimide to the FEP resin is 0.01: 1.
example 3:
FEP film is produced as in example 1 except that:
the concentration of the spermine aqueous solution is 11.2 wt%;
the concentration of the methanol solution of perfluorooctanoic acid was 6.3 wt%;
the mass ratio of the activated polyimide to the perfluorooctanoic acid is 1: 0.34;
the mass ratio of the modified polyimide to the FEP resin is 0.016: 1.
example 4:
preparation of FEP film:
polyimide activation, namely cutting a CPI film, soaking the cut CPI film into 10.5 wt% of polyethylene imine water solution, soaking for 24 hours, washing the cut CPI film with deionized water for multiple times, and drying the cut CPI film at the temperature of 60 ℃ for 12 hours to obtain activated polyimide;
modifying polyimide, namely adding a methanol solution of perfluorooctanoic acid with the concentration of 5.6 wt% into activated polyimide, stirring for reaction for 22 hours, washing for 4 times by using methanol, washing for multiple times by using deionized water, and drying for 12 hours at the temperature of 60 ℃ to obtain modified polyimide; specifically, the mass ratio of the activated polyimide to the perfluorooctanoic acid is 1: 0.32 of;
the FEP composite film is prepared by compounding modified polyimide and FEP resin (the mass ratio of the modified polyimide to the FEP resin is 0.014: 1) and preparing the FEP film with the thickness of 0.18mm by adopting an extrusion casting method. Wherein, the extrusion casting method comprises the following steps: extruding by an extruder, casting by a T-shaped opening die, cooling by an air knife and a cooling roller, carrying out corona treatment, cutting waste edges and coiling; the extrusion process of the extruder has the melting temperature of 280 ℃ and the extrusion temperature of 310 ℃.
Example 5:
preparation of FEP film:
polyimide activation, namely cutting a CPI film, soaking the cut CPI film into a 10.5 wt% concentration spermine aqueous solution, soaking for 7 hours, washing with deionized water for multiple times, and drying at 60 ℃ for 12 hours to obtain activated polyimide;
modifying polyimide, namely adding a 5.6 wt% methanol solution of 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid into activated polyimide, stirring to react for 22H, washing for 4 times by using methanol, washing for multiple times by using deionized water, and drying for 12H at 60 ℃ to obtain modified polyimide; specifically, the mass ratio of the activated polyimide to the perfluorooctanoic acid is 1: 0.32 of;
the FEP composite film is prepared by compounding modified polyimide and FEP resin (the mass ratio of the modified polyimide to the FEP resin is 0.014: 1) and preparing the FEP film with the thickness of 0.18mm by adopting an extrusion casting method. Wherein, the extrusion casting method comprises the following steps: extruding by an extruder, casting by a T-shaped opening die, cooling by an air knife and a cooling roller, carrying out corona treatment, cutting waste edges and coiling; the extrusion process of the extruder has the melting temperature of 280 ℃ and the extrusion temperature of 310 ℃.
Example 6:
the FEP film was made as in example 5 except that:
the concentration of the spermine aqueous solution is 11.2 wt%;
the concentration of the methanol solution of 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid was 6 wt%;
the mass ratio of the activated polyimide to the 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid is 1: 0.28;
the mass ratio of the modified polyimide to the FEP resin is 0.017: 1.
example 7:
the FEP film was made as in example 5 except that:
the concentration of the spermine aqueous solution is 9.8 wt%;
the concentration of the methanol solution of 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid was 5 wt%;
the mass ratio of the activated polyimide to the 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid is 1: 0.22;
the mass ratio of the modified polyimide to the FEP resin is 0.015: 1.
example 8:
the FEP film was made as in example 5 except that: and (3) replacing spermine with polyethyleneimine, and soaking for 24 hours.
Example 9:
FEP film is produced as in example 8 except that:
the concentration of the polyethyleneimine aqueous solution is 11.5 wt%;
the concentration of the methanol solution of 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid was 7 wt%;
the mass ratio of the activated polyimide to the 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid is 1: 0.3;
the mass ratio of the modified polyimide to the FEP resin is 0.019: 1.
comparative example 1:
FEP film is produced as in example 1 except that: instead of the aqueous spermine solution, a solution of ethylenediamine in methanol was used.
Test example 1:
1. infrared testing
And (4) determining the structure of the sample by using a Fourier infrared spectrometer. Wherein the wave number is 4000-500 cm -1 Resolution of 4cm -1 。
The above tests were carried out on the polyimide, the activated polyimide prepared in example 1, and the modified polyimides prepared in examples 1 and 5, and the results are shown in fig. 1. From the analysis in the figure, it can be seen that 1773cm in the IR spectrum of the polyimide after activation with spermine is compared with the IR spectrum test result of the polyimide -1 And 1358cm -1 The intensity of the characteristic absorption peak of carbonyl symmetry vibration and carbon-nitrogen bond vibration in the nearby imide ring is obviously weakened, 1645cm -1 And 1540cm -1 Characteristic absorption peaks of carbonyl stretching vibration and N-H bending vibration in CONH group appear nearby, and are 3321cm -1 And 3040cm -1 The characteristic absorption peak of primary ammonia appears nearby, which indicates that the imide in the polyimide structure is successfully opened by the activation treatment of spermine. 3321cm in the infrared spectrum of the modified polyimide prepared in example 1 -1 And 3040cm -1 The characteristic absorption peak of nearby primary ammonia substantially disappears at 1322cm -1 A C-F bond characteristic absorption peak appears nearby, which indicates that the perfluorooctanoic acid and the activated polyimide successfully undergo amidation reaction. Similarly, 3321cm of the infrared spectrum of the modified polyimide prepared in example 5 -1 And 3040cm -1 The characteristic absorption peak of nearby primary ammonia also basically disappears, 1252cm -1 The characteristic absorption peak of the C-F bond appears nearby, which indicates that 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid and activated polyimide successfully undergo amidation reactionShould be used.
2. Measurement of tensile Strength
The test method was carried out with reference to the standard specified in ASTM-D638.
The above tests were performed on pure FEP resin films, the FEP films prepared in comparative example 1 and examples 1 to 9, and the results are shown in table 1:
table 1 tensile strength test results
From the analysis in table 1, the FEP film compounded with the modified polyimide has a significantly higher tensile strength than the pure FEP resin film. The tensile strength of the FEP film prepared in the embodiment 1 is obviously higher than that of the FEP film prepared in the comparative example 1, and the fact that the polyimide is activated by the spermine and then is subjected to chemical modification is shown, so that when the FEP film is applied to preparation of the FEP film material, the tensile strength of the film material can be effectively improved, and the mechanical property of the FEP film is enhanced. The effect of example 5 is obviously better than that of example 1, and the effect of example 8 is obviously better than that of example 4, which shows that the mechanical property of the membrane material can be further enhanced by using 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid to modify the activated polyimide and compounding the modified polyimide with FEP resin to prepare the membrane material.
3. Test for Friction Properties
The test method is carried out with reference to the standard specified in ASTM F735.
The above tests were performed on pure FEP resin films, the FEP films prepared in comparative example 1 and examples 1 to 9, and the results are shown in table 2:
TABLE 2 Friction Property test results
| Sample (I) | Wear Rate (. times.10) -5 mm 3 ·N -1 ·m -1 ) |
| Pure FEP resin film | 2.93 |
| Comparative example 1 | 2.82 |
| Example 1 | 2.75 |
| Example 2 | 2.76 |
| Example 3 | 2.74 |
| Example 4 | 2.68 |
| Example 5 | 2.73 |
| Example 6 | 2.74 |
| Example 7 | 2.73 |
| Example 8 | 2.55 |
| Example 9 | 2.53 |
From the analysis in table 2, it can be seen that the abrasion rate of the FEP film compounded with the modified polyimide is significantly lower than that of the pure FEP resin film. The wear rate of the FEP film prepared in the embodiment 1 is obviously lower than that of the FEP film prepared in the comparative example 1, and the application of the FEP film prepared in the embodiment 1 in the preparation of the FEP film material by activating the polyimide with spermine and then carrying out chemical modification can effectively improve the wear rate of the film material and enhance the wear performance of the film material. The effect of example 5 is equivalent to that of example 1, and the effect of example 8 is significantly better than that of example 4, which shows that the film material prepared by modifying the polyimide activated by spermine with 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid and compounding the modified polyimide with FEP resin does not negatively affect the friction performance of the film material. The polyimide activated by the 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid modified polyethyleneimine is compounded with FEP resin to prepare the membrane material, and the polyimide and the FEP resin have a synergistic effect, so that the friction performance of the membrane material can be further enhanced.
Test example 2:
1. oxygen permeability test
The tests were carried out according to the standards specified in ASTM D3985. The test instrument measures oxygen transmission rate using an OTR analyzer at 37 ℃.
The above tests were performed on pure FEP resin films, the FEP films prepared in comparative example 1 and examples 1 to 9, and the results are shown in Table 3:
TABLE 3 oxygen permeability test results
| Sample(s) | OTR(cc/(m 2 ·day·atm)) |
| Pure FEP resin film | 2900 |
| Comparative example 1 | 2680 |
| Example 1 | 2750 |
| Example 2 | 2742 |
| Example 3 | 2760 |
| Example 4 | 2820 |
| Example 5 | 2800 |
| Example 6 | 2809 |
| Example 7 | 2796 |
| Example 8 | 2890 |
| Example 9 | 2895 |
From the analysis in table 3, it can be seen that the oxygen permeability of the FEP film compounded with the modified polyimide is slightly reduced compared to the pure FEP resin film. The oxygen permeability of the FEP film prepared in example 1 is higher than that of the FEP film prepared in comparative example 1, which shows that the oxygen permeability of the FEP film material can be slightly improved by activating polyimide with spermine and then carrying out chemical modification. The effect of example 5 is better than that of example 1, and the effect of example 8 is obviously better than that of example 4, which shows that the film material prepared by modifying the polyimide activated by spermine with 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid and compounding the modified polyimide with FEP resin has a certain regulating effect on the oxygen permeability of the film material. The membrane material is prepared by compounding polyimide activated by 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid modified polyethyleneimine and FEP resin, and the polyimide and the FEP resin have a synergistic effect, so that the oxygen permeability of the membrane material can be remarkably improved, and the oxygen permeability of the pure FEP resin membrane is basically achieved.
2. Water vapor permeability test
The tests were carried out according to the standards specified in ASTM F1249. Experiment the water vapour transmission rate of the sample film was measured using a permantan W700 water vapour analyser from MOCON corporation, usa. The average water loss within 14d at 40 ℃ was measured.
The above tests were performed on pure FEP resin films, the FEP films prepared in comparative example 1 and examples 1 to 9, and the results are shown in Table 4:
table 4 water vapor permeability test results
| Sample (I) | Average water loss/%) |
| Pure FEP resin film | 0.23 |
| Comparative example 1 | 1.02 |
| Example 1 | 0.98 |
| Example 2 | 1.00 |
| Example 3 | 0.97 |
| Example 4 | 0.74 |
| Example 5 | 0.86 |
| Example 6 | 0.84 |
| Example 7 | 0.87 |
| Example 8 | 0.48 |
| Example 9 | 0.46 |
From the analysis in table 4, the average moisture loss of FEP film compounded with modified polyimide is increased compared to the pure FEP resin film. The average water loss of the FEP film prepared in example 1 is equivalent to that of comparative example 1, and shows that the polyimide is activated by spermine and then is subjected to chemical modification, so that when the FEP film is applied to preparation of FEP film materials, the water vapor permeability of the film materials is not negatively influenced. The effect of example 5 is better than that of example 1, and the effect of example 8 is obviously better than that of example 4, which shows that 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid is adopted to modify the activated polyimide, and then the polyimide is compounded with FEP resin to prepare the membrane material, so that the water vapor permeability of the membrane material can be obviously reduced, and the loss of water can be reduced.
Test example 3:
light transmission performance test
The light transmittance of the sample films was measured using a UV-Vis-NIR spectrophotometer.
The FEP films prepared in the pure FEP resin film, the comparative example 1, and the examples 1 to 9 were subjected to the above test, and the results are shown in table 5:
TABLE 5 light transmittance test results
| Sample (I) | Visible light transmittance/%) |
| Pure FEP resin film | 95.8 |
| Comparative example 1 | 91.3 |
| Example 1 | 92.0 |
| Example 2 | 91.8 |
| Example 3 | 92.2 |
| Example 4 | 92.5 |
| Example 5 | 92.3 |
| Example 6 | 92.1 |
| Example 7 | 92.2 |
| Example 8 | 92.7 |
| Example 9 | 93.0 |
From the analysis in table 5, the visible light transmittance of the FEP film compounded with the modified polyimide was reduced compared to that of the pure FEP resin film. The light transmittance of the FEP film prepared in example 1 is equivalent to that of comparative example 1, and shows that the polyimide is activated by spermine and then is subjected to chemical modification, so that when the FEP film is applied to preparation of FEP film materials, the light transmittance of the FEP film materials is not negatively influenced. The effect of example 5 is equivalent to that of example 1, and the effect of example 8 is equivalent to that of example 4, which shows that the film material prepared by modifying activated polyimide with 5-methyl-1- (3-trifluoromethylphenyl) -1H-pyrazole-3-carboxylic acid and compounding the modified activated polyimide with FEP resin does not negatively affect the light transmittance of the film material. Although the visible light transmittance of the FEP film prepared by the invention is reduced, the visible light transmittance is still maintained at a higher light transmittance level, and the observation of morphological characteristics and the like in the cell treatment process can be met.
Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. An FEP film which is a composite film material comprising at least an FEP resin, and,
modified polyimide; the modified polyimide comprises products of polyimide activation reaction and amidation reaction; the raw materials for the activation reaction comprise spermine or polyethyleneimine; the amidation reaction raw material comprises perfluorooctanoic acid;
the polyimide is a transparent polyimide material.
2. The FEP film as claimed in claim 1, wherein: the mass ratio of the modified polyimide to the FEP resin is 0.01-0.02: 1.
3. The FEP film as claimed in claim 1, wherein: the tensile strength of the FEP film is more than 24 MPa.
4. The method for producing the FEP film as claimed in claim 1, comprising:
activating polyimide, namely dipping a polyimide material by adopting a spermine or polyethylene imine water solution, and performing an activation reaction to obtain activated polyimide;
modifying polyimide, namely chemically modifying the activated polyimide by adopting perfluorooctanoic acid through amidation reaction to obtain modified polyimide;
preparing the FEP composite film, namely compounding the modified polyimide with the FEP resin, and preparing the FEP composite film by adopting a tape casting method.
5. The method for producing the FEP film as claimed in claim 4, wherein: the concentration of the spermine or polyethyleneimine aqueous solution is 9-12 wt%.
6. The method for producing the FEP film as claimed in claim 4, wherein: the mass ratio of the activated polyimide to the perfluorooctanoic acid is 1: 0.2 to 0.4.
7. Use of the modified polyimide as claimed in claim 1 for enhancing the mechanical properties of FEP films.
8. Use of the FEP film of claim 1 in the preparation of a bag for cell therapy stock solution.
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| US3770566A (en) * | 1971-06-08 | 1973-11-06 | Du Pont | Interdiffusionally bonded structures of polyimide and fluoropolymer |
| US20030070545A1 (en) * | 2001-08-15 | 2003-04-17 | Ye Liu | Chemical modification of polyimides |
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| US3770566A (en) * | 1971-06-08 | 1973-11-06 | Du Pont | Interdiffusionally bonded structures of polyimide and fluoropolymer |
| US20030070545A1 (en) * | 2001-08-15 | 2003-04-17 | Ye Liu | Chemical modification of polyimides |
| TW200831089A (en) * | 2006-11-17 | 2008-08-01 | Pfizer | Substituted bicyclocarboxyamide compounds |
| CN107208014A (en) * | 2014-12-22 | 2017-09-26 | 美国圣戈班性能塑料公司 | cell capture system and method |
| CN109337108A (en) * | 2018-10-26 | 2019-02-15 | 镇江龙成绝缘材料有限公司 | A kind of polyimides-perfluoroethylene-propylene preparation method of composite film |
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