CN114988576B - Method for rapidly realizing short-cut denitrification by taking domestic sewage as carbon source through anoxic starvation - Google Patents

Method for rapidly realizing short-cut denitrification by taking domestic sewage as carbon source through anoxic starvation Download PDF

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CN114988576B
CN114988576B CN202210720040.8A CN202210720040A CN114988576B CN 114988576 B CN114988576 B CN 114988576B CN 202210720040 A CN202210720040 A CN 202210720040A CN 114988576 B CN114988576 B CN 114988576B
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starvation
anoxic
sludge
reactor
treatment
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CN114988576A (en
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彭永臻
王峥嵘
李翔晨
张静雯
李夕耀
王淑莹
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Beijing University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/30—Aerobic and anaerobic processes
    • C02F3/302—Nitrification and denitrification treatment
    • C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/06—Controlling or monitoring parameters in water treatment pH
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/22—O2
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

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  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
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  • Environmental & Geological Engineering (AREA)
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Abstract

A method for rapidly realizing short-cut denitrification by taking domestic sewage as a carbon source through oxygen-deficient starvation belongs to the field of biological sewage treatment. The method comprises the following steps: 1. closing the water inlet and outlet system, continuously stirring, and maintaining an anoxic starvation state; 2. starving for 8-20 days under oxygen deficiency; 3. and recovering the sludge activity. The method inhibits the expression level of functional genes of nitrite reductase through starvation treatment, the expression of the functional genes of nitrate reductase is basically not influenced, short-range denitrification can be realized by using organic matters in domestic sewage as a carbon source in a short period, and the short-range denitrification can be stably maintained for a long time by controlling the retention time of a water body and the sludge concentration in the operation period. The method is simple to operate, convenient and fast to control, capable of quickly realizing short-range denitrification and maintaining a high nitrite accumulation rate for a long time, and wide in application prospect.

Description

Method for rapidly realizing short-cut denitrification by taking domestic sewage as carbon source through anoxic starvation
The technical field is as follows:
the invention relates to a method for quickly realizing short-cut denitrification by taking domestic sewage as a carbon source through oxygen-poor starvation, belonging to the field of biological sewage treatment.
Background art:
with the rapid development of human activities and society, the ecological environment situation of the world is more severe nowadays. The water environment pollution problem in China is particularly prominent, water eutrophication caused by nitrogen and phosphorus and other nutrient elements is increasingly aggravated in recent years, and the pollutant discharge standard in China is increasingly strict. The traditional nitrification and denitrification process needs a large amount of aeration, and simultaneously needs to add a large amount of external carbon sources in order to meet the requirement of effluent, thereby obviously increasing the cost of sewage treatment.
As an efficient autotrophic nitrogen removal process, the anaerobic ammonia oxidation process has the advantages of carbon source saving, no need of aeration, low sludge yield and the like, but the application of the anaerobic ammonia oxidation process in mainstream municipal domestic sewage faces the limitation of nitrite substrate deficiency. At present, two processes of short-cut nitrification and short-cut denitrification are mainly used for providing a nitrite substrate for anaerobic ammonia oxidation, the short-cut nitrification has the limitations of difficult start, harsh maintenance conditions, difficult thorough elutriation of NOB and the like in application, and the short-cut denitrification has the advantages of simple start, stable operation, synchronous removal of organic matters in sewage and the like and has wide application prospect.
At present, the main control strategies for realizing the short-cut denitrification include adding biodegradable organic matters, maintaining higher pH, adding hydroxylamine and the like, and the control strategies are more complicated and increase the operation cost for realizing the short-cut denitrification.
The anaerobic starvation treatment is very convenient and fast in operation, anaerobic starvation can be directly realized through an idle reactor, the functional gene expression level of the nitrite reductase is inhibited through the anaerobic starvation, and the functional gene expression of the nitrate reductase is basically not influenced, so that higher nitrite accumulation is quickly realized. In subsequent operation, the short sludge age is controlled to maintain the low sludge concentration, the ratio of the influent domestic sewage to the nitrate wastewater is adjusted, the influent C/N is controlled to be 3, and the short-cut denitrification can be realized in a short time.
The invention content is as follows:
the invention aims to provide a method for quickly realizing short-cut denitrification by taking domestic sewage as a carbon source. The invention utilizes the function gene expression level of inhibiting nitrite reductase under the condition of oxygen deficiency and the function gene expression of nitrate reductase is basically not influenced, thereby enlarging the difference between the reduction rate of nitrate and the reduction rate of nitrite, further realizing higher nitrite accumulation, assisting in controlling sludge age and C/N, and quickly realizing short-cut denitrification by taking domestic sewage as a carbon source.
A method for rapidly realizing short-cut denitrification by taking domestic sewage as a carbon source through oxygen-deficient starvation adopts a device mainly comprising the following steps: an SBR reactor (11) and online pH and DO detection equipment; as shown in figure 1, the SBR reactor (11) enters domestic sewage and nitrate wastewater through a water inlet system (1), and a stirrer (2) is arranged for stirring to uniformly mix activated sludge in the reactor; water is discharged through a water outlet valve (5); a drain valve I (3), a drain valve II (4), a drain valve III (6) and a drain valve IV (7) are arranged on the side wall of the SBR reactor (11); the pH probe (9) and the DO probe (10) are connected with the WTW host (8) through data lines, and can reflect the sludge property in the reactor.
A method for rapidly realizing short-cut denitrification by taking domestic sewage as a carbon source through anoxic starvation is characterized by comprising the following steps:
(1) Before anoxic starvation treatment, inoculating excess sludge of a sewage treatment plant, controlling the sludge concentration to be 1500-2500 mg/L, and debugging a reactor to ensure the normal operation of each device;
(2) Performing anoxic starvation treatment, closing a water inlet system and a water outlet system of the reactor, adding no matrix, and controlling the stirrer to be in a normally open state to enable the reactor to be in an anoxic starvation state for a long time; sampling periodically to determine the state and material change of sludge in the reactor, determining anoxic starvation time according to the property of the sludge, determining the nitrite accumulation rate of the system after starvation treatment by a batch experimental method, stopping anoxic starvation treatment when batch experiments show that the nitrite accumulation rate of the system reaches more than 80%, and performing active recovery and stable operation stages;
(3) Restarting the reactor to quickly recover the activity of the denitrifying bacteria: firstly, performing centrifugal treatment on sludge subjected to starvation treatment to remove substances such as nitrogen and phosphorus generated by fermentation in an anoxic starvation process; opening a water inlet system and a water drainage system, operating the SBR system according to a preset program, and operating four periods every day, wherein each period is four hours, and the four periods mainly comprise water inlet 10min, anoxic stirring 180min, sedimentation 30min and water drainage 20min, and the water drainage ratio is 50%; in the stable operation stage, keeping the sludge age of 10-15 days; adjusting the proportion of the influent domestic sewage to the nitrate wastewater, and controlling the influent C/N to be 3; when the concentration of nitrate nitrogen at the end of the reaction reaches below 5mg/L before starvation treatment, the reactor is considered to be restored to activity and begins to enter a stable operation stage;
the technical principle of the invention is as follows: the functional gene expression level of the nitrite reductase is inhibited under the condition of oxygen deficiency starvation, and the functional gene expression of the nitrate reductase is basically not influenced, so that the difference between the nitrate reduction rate and the nitrite reduction rate is enlarged, higher nitrite accumulation is realized, sludge age and C/N are controlled, and short-cut denitrification with domestic sewage as a carbon source is quickly realized.
Compared with the prior art for realizing the short-range denitrification, the method for quickly realizing the short-range denitrification by taking the domestic sewage as the carbon source through the anoxic starvation has the following advantages:
organic matters in the domestic sewage can be directly utilized to drive short-range denitrification, and the organic matters in the domestic sewage are synchronously removed;
the start-up time of the short-cut denitrification is short. By the anoxic starvation treatment, the expression level of the functional gene of the nitrite reductase is quickly inhibited, the expression of the functional gene of the nitrate reductase is basically not influenced, and the reactor can immediately show higher nitrite accumulation after being restarted;
the anoxic starvation treatment method is simple and does not need complex operation. In the anoxic starvation treatment process, only stirring is needed, and a complex control process is not needed, so that the anoxic starvation treatment process is convenient to realize;
the cost for realizing the short-range denitrification is lower. Short-range denitrification with domestic sewage as a carbon source is realized through anoxic starvation treatment, an external carbon source and other agents are not required to be added, and the economic cost is low;
the invention has stronger practicability and can quickly realize short-cut denitrification in an SBR reactor in a sewage treatment plant through idling for a certain time.
Description of the drawings:
FIG. 1 is a schematic structural diagram of a process for rapidly carrying out short-cut denitrification using domestic sewage as a carbon source by anoxic starvation treatment.
In the figure: 1 is a water inlet system, 2 is a stirrer, 3 is a drain valve I, 4 is a drain valve II, 5 is a water outlet valve, 6 is a drain valve III, 7 is a drain valve IV, 8 is a WTW host, 9 is a pH probe, 10 is a DO probe, and 11 is an SBR reactor.
FIG. 2 shows the results of the experiments performed eight days after the anaerobic starvation treatment.
The specific implementation mode is as follows:
the present invention will be described in further detail with reference to the drawings and technical solutions, but the present invention is not limited to the following embodiments.
Referring to fig. 1, a schematic diagram of a method and an apparatus for rapidly realizing short-cut denitrification by using domestic sewage as a carbon source through anoxic starvation, wherein an SBR reactor (11) enters the domestic sewage and nitrate wastewater through a water inlet system (1), and a stirrer (2) is arranged for stirring to uniformly mix activated sludge in the reactor; water is discharged through a water outlet valve (5); the side wall of the SBR reactor (11) is provided with a drain valve I (3), a drain valve II (4), a drain valve III (6) and a drain valve IV (7); the pH probe (9) and the DO probe (10) are connected with the WTW host (8) through data lines, and can reflect the sludge property in the reactor.
Referring to fig. 1, a schematic diagram of a method and an apparatus for rapidly realizing short-cut denitrification by using domestic sewage as a carbon source through anoxic starvation, the short-cut denitrification is realized according to the following steps:
(1) Before anoxic starvation treatment, inoculating excess sludge of a sewage treatment plant, controlling the sludge concentration to be 1500-2500 mg/L, and debugging a reactor to ensure the normal operation of each device;
(2) Performing anoxic starvation treatment, closing a water inlet system and a water outlet system of the reactor, adding no matrix, and controlling the stirrer to be in a normally open state to enable the reactor to be in an anoxic starvation state for a long time; sampling periodically to determine the state and material change of sludge in the reactor, determining anoxic starvation time according to the property of the sludge, determining the nitrite accumulation rate of the system after starvation treatment by a batch experimental method, stopping anoxic starvation treatment when batch experiments show that the nitrite accumulation rate of the system reaches more than 80%, and performing active recovery and stable operation stages;
(3) Restarting the reactor to quickly recover the activity of the denitrifying bacteria: firstly, performing centrifugal treatment on sludge subjected to starvation treatment to remove substances such as nitrogen, phosphorus and the like generated by fermentation in an anoxic starvation process; opening a water inlet system and a water drainage system, operating the SBR system according to a preset program, and operating four periods every day, wherein each period is four hours, and the four periods mainly comprise water inlet 10min, anoxic stirring 180min, sedimentation 30min and water drainage 20min, and the water drainage ratio is 50%; in the stable operation stage, keeping the sludge age for 10 to 15 days; adjusting the proportion of the influent domestic sewage to the nitrate wastewater, and controlling the influent C/N to be 3; when the concentration of nitrate nitrogen at the end of the reaction reaches below 5mg/L before starvation treatment, the reactor is considered to be restored to activity and begins to enter a stable operation stage;
the process of the present invention is further illustrated below with reference to batch experimental results:
the excess sludge of a certain sewage treatment plant is used as inoculation sludge, domestic sewage discharged by a certain university family area is diluted to be used as a carbon source, starvation treatment is carried out, and sludge is taken regularly to carry out batch experiments, so that the figure shows that anoxic starvation can obviously improve the nitrite accumulation rate in the denitrification process, and the NTR is improved from 46.55% to 61.02% after eight days of anoxic starvation; the nitrate reduction rate is not inhibited after the anoxic starvation treatment; the significant inhibition of nitrite reduction rate over the entire period of the anoxic starvation treatment is the main reason for the increase of NTR.
The device and the method for quickly starting the denitrification of the anaerobic ammonium oxidation coupled with the short-cut denitrification through the addition of the diatomite are described in detail, the principle and the implementation mode of the invention are explained by applying specific examples, and the explanation of the implementation examples is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and applications, and in summary, the content of the present specification should not be construed as a limitation to the present invention.

Claims (1)

1. A method for rapidly realizing short-cut denitrification by taking domestic sewage as a carbon source through anoxic starvation adopts a device comprising the following steps: an SBR reactor (11) and online pH and DO detection equipment; the SBR reactor (11) enters the domestic sewage and the nitrate wastewater through the water inlet system (1), and a stirrer (2) is arranged for stirring to uniformly mix the activated sludge in the reactor; water is discharged through a water outlet valve (5); a drain valve I (3), a drain valve II (4), a drain valve III (6) and a drain valve IV (7) are arranged on the side wall of the SBR reactor (11); the pH probe (9) and the DO probe (10) are connected with the WTW host (8) through data lines;
the method is characterized by comprising the following steps:
(1) Inoculating the excess sludge of the sewage treatment plant before the anoxic hunger treatment, and controlling the sludge concentration to be 1500-2500 mg/L;
(2) Performing anoxic starvation treatment, closing a water inlet system and a water outlet system of the reactor, adding no matrix, and controlling the stirrer to be in a normally open state to enable the reactor to be in an anoxic starvation state for a long time; sampling at regular intervals to determine the state and material change of sludge in the reactor, determining the anoxic starvation time according to the property of the sludge, determining the nitrite accumulation rate of the system after the starvation treatment by a batch experimental method, stopping performing the anoxic starvation treatment when the batch experiment shows that the nitrite accumulation rate of the system reaches more than 80%, and performing an active recovery and stable operation stage;
(3) Restarting the reactor to quickly recover the activity of the denitrifying bacteria: firstly, carrying out centrifugal treatment on the sludge subjected to starvation treatment to remove nitrogen and phosphorus generated by fermentation in the anoxic starvation process; opening a water inlet system and a water drainage system, operating the SBR system according to the following procedures, and operating four periods every day, wherein each period is four hours, and comprises water inlet 10min, anoxic stirring 180min, precipitation 30min and water drainage 20min, and the water drainage ratio is 50%; in the stable operation stage, keeping the sludge age of 10-15 days to maintain the sludge concentration at 1500-2500 mg/L; adjusting the proportion of the influent domestic sewage to the nitrate wastewater, and controlling the influent C/N to be 3; when the concentration of the nitrate nitrogen at the end of the reaction reaches below 5mg/L before starvation treatment, the SBR reactor is considered to be restored in activity and begins to enter a stable operation stage.
CN202210720040.8A 2022-06-24 2022-06-24 Method for rapidly realizing short-cut denitrification by taking domestic sewage as carbon source through anoxic starvation Active CN114988576B (en)

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