CN114984664B - Method for filtering viscous konjaku flour enzymatic hydrolysate - Google Patents
Method for filtering viscous konjaku flour enzymatic hydrolysate Download PDFInfo
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- 229920002752 Konjac Polymers 0.000 title claims abstract description 82
- 238000001914 filtration Methods 0.000 title claims abstract description 63
- 235000013312 flour Nutrition 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000002255 enzymatic effect Effects 0.000 title claims abstract description 29
- 239000000413 hydrolysate Substances 0.000 title claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 86
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 235000010485 konjac Nutrition 0.000 claims description 33
- 239000011259 mixed solution Substances 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 15
- 108010055059 beta-Mannosidase Proteins 0.000 claims description 13
- 102100032487 Beta-mannosidase Human genes 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 10
- 238000000967 suction filtration Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 10
- 235000005822 corn Nutrition 0.000 claims 10
- 230000008901 benefit Effects 0.000 abstract description 6
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 6
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 6
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 abstract description 2
- 238000011085 pressure filtration Methods 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 7
- 238000002156 mixing Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 description 3
- 229920002581 Glucomannan Polymers 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 229940046240 glucomannan Drugs 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 235000019362 perlite Nutrition 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001278826 Amorphophallus Species 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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- 238000007796 conventional method Methods 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D37/00—Processes of filtration
- B01D37/02—Precoating the filter medium; Addition of filter aids to the liquid being filtered
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
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- A61L2/04—Heat
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
- B01D35/18—Heating or cooling the filters
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D37/00—Processes of filtration
- B01D37/02—Precoating the filter medium; Addition of filter aids to the liquid being filtered
- B01D37/025—Precoating the filter medium; Addition of filter aids to the liquid being filtered additives incorporated in the filter
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- C12P19/00—Preparation of compounds containing saccharide radicals
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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Abstract
The invention discloses a method for filtering viscous konjaku flour enzymatic hydrolysate, and belongs to the technical field of turbid liquid treatment. The filtering method comprises enzymolysis of konjaku flour, pretreatment of filtering and filtering. According to the method for filtering the viscous konjaku flour enzymatic hydrolysate, the corncob powder is mixed with the enzymatic hydrolysate after konjaku flour enzymatic hydrolysis and is pre-paved on the surface of the filtering container, and the viscous solution after konjaku flour enzymatic hydrolysis is subjected to pressure filtration under the actions of physical adsorption, elastic aperture and temperature, so that the viscous konjaku enzymatic hydrolysate can be rapidly separated, impurities can be effectively removed, the corncob powder is food, and the edible safety is higher. Compared with the prior art, the method has the advantages of high filtering speed, no blockage, obvious improvement of filtering efficiency, reduction of production cost, simple operation and economy and effectiveness.
Description
Technical Field
The invention belongs to the technical field of turbid liquid treatment, and particularly relates to a method for filtering viscous konjaku flour enzymatic hydrolysate.
Background
Konjak (Amorphophallus konjak) is a special cash crop in the southern area of China, and the main component of konjak glucomannan (konjak glucomannan, KGM). The konjak glucomannan mainly comprises mannose and glucose, and the molar ratio is 1.5:1, it has been shown by studies that it can be hydrolysed by beta-endoglucanases and beta-mannanases. At present, konjaku flour is mainly eaten as crude fiber or used as a simple food additive in China, and the added value is not high. The konjak flour is hydrolyzed to prepare the mannose oligomer, so that the application range of the konjak flour can be greatly widened, the added value of raw materials is improved, and the konjak flour has great economic and social benefits.
At present, the preparation of the mannose oligomer generally utilizes high-temperature hydrolysis, acid hydrolysis or alkali hydrolysis and enzymatic hydrolysis, and the enzymatic hydrolysis is the most common method, namely, after the konjak flour is subjected to mannase enzymolysis, the mannose oligomer can be obtained through processes such as separation, purification and the like, and the mannose oligomer can be used as functional food, additive, even medicines and the like, and has wide application. However, the solution of konjak powder after enzymolysis by mannanase is very viscous and is easy to form a solidified film, and the enzymolysis liquid is difficult to effectively separate due to the viscous characteristic of the konjak powder, so that the high-efficiency filtration separation has a critical influence on the quality of the prepared mannose oligomer, the existing solid-liquid separation technology usually adopts methods such as plate-frame filter pressing, vacuum drum suction filtration, centrifugal machine centrifugation and the like, and filter aids are mainly diatomite, perlite, lignocellulose and the like in the process, but the combination of the methods and the filter aids has low filtration efficiency and unsatisfactory separation effect on the enzymolysis liquid of the konjak powder, and even cannot realize solid-liquid separation.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for filtering viscous konjak powder enzymatic hydrolysate, which has the advantages of low cost, easy operation and good separation effect.
The aim of the invention is achieved by the following technical scheme: a method for filtering viscous konjak powder enzymatic hydrolysate comprises the following steps:
s1, enzymolysis of konjaku flour: adding mannase into konjaku flour, stirring uniformly, adding water with the temperature of 45-55 ℃ for enzymolysis for at least 4 hours, and controlling the pH value of the solution to be 5-6 in the enzymolysis process to obtain viscous konjaku flour enzymolysis liquid;
since konjak flour swells and forms a viscous liquid having a viscosity of 10000CPS or more when mixed with water, it becomes a gel-like substance easily at pH > 7. Therefore, the pH value of the solution is required to be kept between 5 and 6 during enzymolysis, the gelation process of konjak and water after mixing is prevented, the viscosity of konjak is greatly reduced, and the enzymolysis effect and the filterability of subsequent solutions are improved.
S2, filtering pretreatment: adding corncob powder into the viscous konjaku flour enzymolysis liquid obtained in the step S1, uniformly stirring to obtain a mixed solution, heating the mixed solution to 80-85 ℃, and preserving heat for 25-35 min; the treatment ensures that the corncob powder is fully expanded, and particles in the solution can be effectively adsorbed; can complete the disinfection of corncob powder, and simultaneously, the active enzyme used by enzymolysis is eliminated, thereby improving the stability of the product.
S3, filtering: the temperature of the mixed solution is kept at 75-80 ℃, the mixed solution is poured into a filtering container with the surface pre-paved with corncob powder for filtering, and the clarified filtrate is the filtered konjaku flour enzymatic hydrolysate.
The mixed solution contains a plurality of components such as products (oligomannose), starch, fiber, impurities and the like after enzymolysis, and the mixed solution is water-soluble or water-insoluble, and the solution is required to be filtered to remove the water-insoluble components, so that the main product, the oligomannose after enzymolysis is obtained. In the filtering process, along with the decrease of the temperature, the solution containing konjak can form a film to obstruct the circulation of liquid, so that the filtering speed is slow, the temperature of the solution in the filter is kept at 75-80 ℃, the continuous disinfection effect can be effectively achieved at the temperature, meanwhile, the Maillard reaction of sugar contained in the solution has the smallest influence on the color of the solution at the temperature, the filtering speed of the solution is fast at the temperature, the surface of the filter medium is not easy to form a film, and the filter medium keeps good permeability.
Further, the amount ratio of the mannanase to the konjaku flour in the step S1 is 300U-600 U:1g.
Further, in the step S1, the weight ratio of the water to the konjaku flour is 8-9:1.
Further, in the step S2, the corncob powder is coarse corncob powder, and the mesh number of the coarse corncob powder is 60-80 meshes.
Further, in the step S2, the volume ratio of the mass of the corncob powder to the viscous konjaku flour enzymatic hydrolysate is 1g:90 mL-110 mL.
Further, the corncob powder pre-paved in the filtering container in the step S3 is coarse corncob powder and fine corncob powder according to the weight ratio of 1:8-11, the mesh number of the coarse corncob powder is 60-80 meshes, and the mesh number of the fine corncob powder is 80-100 meshes.
Further, the mesh number of the filter container is 350-450 mesh.
Further, in step S3, the specific operation of pre-paving the corncob powder in the filtering container is as follows: adding water into the corncob powder to prepare a suspension with the mass percentage concentration of 2.5-3.5%, uniformly stirring, heating to 80-85 ℃, maintaining for at least 30min, pouring into a filter container to intercept the corncob powder, and obtaining a filter container pre-coated with the corncob powder.
Further, the filtering is performed by suction filtration or pressurization.
The invention has the following advantages: according to the method for filtering the viscous konjaku flour enzymatic hydrolysate, the corncob powder is mixed with the enzymatic hydrolysate after konjaku flour enzymatic hydrolysis and is pre-paved on the surface of the filtering container, and the viscous solution after konjaku flour enzymatic hydrolysis is subjected to pressure filtration under the actions of physical adsorption, elastic aperture and temperature, so that the viscous konjaku enzymatic hydrolysate can be rapidly separated, impurities can be effectively removed, the corncob powder is plant crude fiber, and the edible safety is higher. Compared with the prior art, the method has the advantages of high filtering speed, no blockage, obvious improvement of filtering efficiency, reduction of production cost, simple operation and economy and effectiveness.
Detailed Description
The invention will be further described with reference to examples, but the scope of the invention is not limited to the following:
example 1: a method for filtering viscous konjak powder enzymatic hydrolysate comprises the following steps:
s1, enzymolysis of konjaku flour: adding mannase into konjak powder, stirring uniformly, adding water at 45 ℃ for enzymolysis for at least 4 hours, and controlling the pH value of the solution to be 5 in the enzymolysis process to obtain viscous konjak powder enzymolysis liquid; wherein the weight ratio of the mannanase to the konjaku flour is 300U to 1g, and the weight ratio of the water to the konjaku flour is 8 to 1.
S2, filtering pretreatment: adding 60-mesh crude corncob powder into the viscous konjaku flour enzymolysis liquid in the step S1, wherein the volume ratio of the mass of the corncob powder to the viscous konjaku flour enzymolysis liquid is 1g:90mL, stirring uniformly to obtain a mixed solution, heating the mixed solution to 80 ℃, and preserving heat for 25min;
s3, filtering: the temperature of the mixed solution is kept at 75 ℃, the mixed solution is poured into a 350-mesh filter container with the corncob powder pre-paved on the surface and is filtered by suction filtration, and the clarified filtrate is the filtered konjaku flour enzymatic hydrolysate.
The specific operation of pre-paving corncob powder in the filtering container is as follows: mixing coarse corncob powder and fine corncob powder according to a weight ratio of 1:8, adding water after mixing to prepare a suspension with a mass percentage concentration of 2.5%, wherein the mesh number of the coarse corncob powder is 60 meshes, the mesh number of the fine corncob powder is 80 meshes, heating to 80 ℃ after uniformly stirring, maintaining for at least 30min, pouring into a filter container to intercept the corncob powder, and obtaining a filter container pre-coated with the corncob powder.
Example 2: a method for filtering viscous konjak powder enzymatic hydrolysate comprises the following steps:
s1, enzymolysis of konjaku flour: adding mannase into konjak powder, stirring uniformly, adding water with the temperature of 55 ℃ for enzymolysis for at least 4 hours, and controlling the pH value of the solution to be 6 in the enzymolysis process to obtain viscous konjak powder enzymolysis liquid; wherein the weight ratio of mannanase to konjaku flour is 600U to 1g, and the weight ratio of water to konjaku flour is 9 to 1.
S2, filtering pretreatment: adding 80-mesh crude corncob powder into the viscous konjaku flour enzymolysis liquid in the step S1, wherein the volume ratio of the mass of the corncob powder to the viscous konjaku flour enzymolysis liquid is 1g to 110mL, uniformly stirring to obtain a mixed solution, heating the mixed solution to 85 ℃, and preserving heat for 35min;
s3, filtering: the temperature of the mixed solution is kept at 80 ℃, the mixed solution is poured into a 450-mesh filter container with the corncob powder pre-paved on the surface, and the mixed solution is filtered through suction filtration or pressurization, and the clarified filtrate is the filtered konjaku flour enzymatic hydrolysate.
The specific operation of pre-paving corncob powder in the filtering container is as follows: mixing coarse corncob powder and fine corncob powder according to a weight ratio of 1:11, adding water after mixing to prepare a suspension with a mass percentage concentration of 3.5%, wherein the mesh number of the coarse corncob powder is 80 meshes, the mesh number of the fine corncob powder is 100 meshes, heating to 85 ℃ after uniformly stirring, maintaining for at least 30min, pouring into a filter container to intercept the corncob powder, and obtaining a filter container pre-coated with the corncob powder.
Example 3: a method for filtering viscous konjak powder enzymatic hydrolysate comprises the following steps:
s1, enzymolysis of konjaku flour: adding mannase into konjak powder, stirring uniformly, adding water with the temperature of 50 ℃ for enzymolysis for at least 4 hours, and controlling the pH value of the solution to be 5.5 in the enzymolysis process to obtain viscous konjak powder enzymolysis liquid; wherein the weight ratio of mannanase to konjaku flour is 400U to 1g, and the weight ratio of water to konjaku flour is 8.5 to 1.
S2, filtering pretreatment: adding 70-mesh crude corncob powder into the viscous konjaku flour enzymolysis liquid in the step S1, wherein the volume ratio of the mass of the corncob powder to the viscous konjaku flour enzymolysis liquid is 1g to 100mL, stirring uniformly to obtain a mixed solution, heating the mixed solution to 82 ℃, and preserving heat for 30min;
s3, filtering: the temperature of the mixed solution is kept at 78 ℃, the mixed solution is poured into a 400-mesh filter container with the corncob powder pre-paved on the surface, and the mixture is filtered by suction filtration or pressurization, and the clarified filtrate is the filtered konjaku flour enzymatic hydrolysate.
The specific operation of pre-paving corncob powder in the filtering container is as follows: mixing coarse corncob powder and fine corncob powder according to a weight ratio of 1:10, adding water after mixing to prepare a suspension with a mass percentage concentration of 3.0%, wherein the mesh number of the coarse corncob powder is 70 meshes, the mesh number of the fine corncob powder is 100 meshes, heating to 83 ℃ after uniformly stirring, maintaining for at least 30min, pouring into a filter container to intercept the corncob powder, and obtaining a filter container pre-coated with the corncob powder.
Because of the characteristics of konjaku flour and mannose oligomer, the solution after enzymolysis is very viscous, and gel films are easy to form to block filter cloth. The diatomite precoat is easy to be blocked by adopting the traditional filter aid diatomite, perlite and the like for filtration, and the filtration operation can not be completed. The novel filter aid medium lignocellulose is adopted for filtering, the stability of the precoat is poor, the precoat is easy to break up in the filtering process, the filtering effect is poor, the subsequent treatment is difficult, the medium price is high, and the quality of raw materials cannot be controlled. The specific filtering time, raw material price, source, consumption, filtering effect and the like are shown in table 1:
TABLE 1 comparison of different filter aids
As shown in Table 1, compared with the conventional method, the method of the invention has the advantages of 1) wide sources of corncob powder, effective impurity removal, 2) improved filtration efficiency, low production cost, 3) no chemical treatment of the corncob powder and higher edible safety.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art who is skilled in the art to which the present invention pertains will appreciate that the technical scheme and the inventive concept according to the present invention are equally substituted or changed within the scope of the present invention.
Claims (6)
1. The method for filtering the viscous konjak powder enzymatic hydrolysate is characterized by comprising the following steps of:
s1, enzymolysis of konjaku flour: adding mannase into konjaku flour, stirring uniformly, adding water with the temperature of 45-55 ℃ for enzymolysis for at least 4 hours, and controlling the pH value of the solution to be 5-6 in the enzymolysis process to obtain viscous konjaku flour enzymolysis liquid;
s2, pretreatment of filtration: taking corn cob powder, wherein the corn cob powder is coarse corn cob powder, the mesh number of the coarse corn cob powder is 60-80 meshes, adding the coarse corn cob powder into the viscous konjak powder enzymatic hydrolysate obtained in the step S1, uniformly stirring to obtain a mixed solution, heating the mixed solution to 80-85 ℃, and preserving heat for 25-35 min;
s3, filtering: maintaining the temperature of the mixed solution at 75-80 ℃, pouring the mixed solution into a filtering container with the surface pre-paved with corncob powder for filtering, wherein the clarified filtrate is filtered konjaku flour enzymatic hydrolysate; the corn cob powder pre-paved in the filtering container is coarse corn cob powder and fine corn cob powder according to the weight ratio of 1:8-11, the mesh number of the coarse corn cob powder is 60-80 meshes, and the mesh number of the fine corn cob powder is 80-100 meshes; the specific operation of pre-paving corncob powder in the filtering container is as follows: adding water into the corncob powder to prepare a suspension with the mass percentage concentration of 2.5-3.5%, uniformly stirring, heating to 80-85 ℃, maintaining for at least 30min, pouring into a filter container to intercept the corncob powder, and obtaining a filter container pre-coated with the corncob powder.
2. The method for filtering an enzymatic hydrolysate of viscous konjaku flour according to claim 1, characterized in that the amount ratio of mannanase to konjaku flour in step S1 is 300U-600 u:1g.
3. The method for filtering an enzymatic hydrolysate of viscous konjaku flour according to claim 1, characterized in that the weight ratio of water to konjaku flour in step S1 is 8-9:1.
4. The method for filtering an enzymatic hydrolysate of viscous konjak flour according to claim 1, characterized in that the volume ratio of the mass of the corncob powder to the enzymatic hydrolysate of viscous konjak flour in step S2 is 1g:90 mL-110 mL.
5. The method for filtering an enzymatic hydrolysate of viscous konjak powder according to claim 1, characterized in that the mesh number of the filtering container is 350-450 mesh.
6. The method for filtering an enzymatic hydrolysate of viscous konjak flour as claimed in claim 1, wherein the filtering is performed by suction filtration or pressurization.
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