CN114984309A - Preparation method and application of amphoteric ion polymer modified nanosheet - Google Patents

Preparation method and application of amphoteric ion polymer modified nanosheet Download PDF

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CN114984309A
CN114984309A CN202210597817.6A CN202210597817A CN114984309A CN 114984309 A CN114984309 A CN 114984309A CN 202210597817 A CN202210597817 A CN 202210597817A CN 114984309 A CN114984309 A CN 114984309A
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psbma
mos
pda
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谢婧
俞鹏
李建树
丁春梅
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Sichuan University
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Abstract

The invention discloses a preparation method of a nano sheet modified by a zwitterionic polymer. The invention also discloses the zwitterionic polymer modified nanosheet MoS2@ PDA @ PSBMA prepared by the preparation method and application thereof in osteoarthritis treatment. The invention prepares MoS by using an ultrasonic method 2 Nano-sheets, and then carrying out dopamine modification on the nano-sheets; preparing a block PSBMA-b-PNHSMA copolymer by a RAFT method; combining PSBMA-b-PNHSMA block polymer withThe dopamine-modified nanosheet is prepared by a one-pot method to obtain a zwitterionic polymer-modified nanosheet MoS2@ PDA @ PSBMA. In the preparation process, various functional components can be mutually promoted, so that the biological function can be better exerted, the prepared MoS2@ PDA @ PSBMA nano-sheet can eliminate the over-expressed active oxygen in the inflammatory environment, and the lubricating property is excellent to protect the articular cartilage; and the preparation process is simple, the reaction condition is mild, no special equipment is needed, and the method is suitable for industrial production and application and popularization.

Description

Preparation method and application of zwitterionic polymer modified nanosheet
Technical Field
The invention relates to the field of high polymer materials, in particular to a preparation method and application of a nano sheet modified by a zwitterionic polymer.
Background
Degenerative Osteoarthritis (OA) is primarily characterized by loss of joint lubricating function and by inflammatory overexpression. Loss of joint lubrication can cause articular cartilage to act on high friction for a long time, causing articular cartilage damage and cartilage matrix degradation; inflammatory overexpression, which is mainly manifested by increased levels of inflammatory factors, degradative enzymes, and Reactive Oxygen Species (ROS), also results in decreased chondrocyte activity, affecting extracellular matrix secretion. ROS mainly comprise hydrogen peroxide (H) 2 O 2 ) Hydroxyl radical (. OH) and superoxide anion (O) 2 · -) etc., their overexpression causes oxidative modification of amino acids, which in turn destroys the structure of the protein and ultimately causes cartilage damage. Therefore, achieving both remodeling of joint lubrication function and scavenging of active oxygen is of great significance for the treatment of OA.
Since the joint space is a closed system, joint space injection is one of the main means of treating OA. Currently, the joint cavity injection preparations are mainly high molecular weight and ultrahigh molecular weight hyaluronic acid. Although these agents have some joint lubrication and inflammatory regulation functions, they are not able to scavenge active oxygen that is overexpressed in the joint cavity. In addition, OA also promotes hyaluronidase production, and the therapeutic effect of hyaluronic acid injection into the joint cavity generally lasts only about 3 months, and can be sustained only by repeated injection. Therefore, injecting a material that is lubricious, resistant to oxidation (active oxygen scavenging) and not affected by degrading enzymes into the joint cavity can more effectively achieve OA treatment.
Disclosure of Invention
The invention aims to solve the problems of poor antioxidant capacity, easy degradation and short curative effect maintaining time of the existing joint cavity injection preparation for treating osteoarthritis, provides a preparation method of a nano sheet modified by a zwitterionic polymer, and the prepared nano sheet modified by the zwitterionic polymer has a very wide application prospect in osteoarthritis treatment.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a preparation method of a zwitterionic polymer modified nanosheet comprises the following steps:
s1, taking molybdenum disulfide (MoS) 2 ) Dissolving the powder in mixed solvent of ethanol and water, and ultrasonically preparing MoS 2 Nanosheets;
s2, dissolving macroinitiators PSBMA macro CTA, Azobisisobutyronitrile (AIBN) and methacrylic acid (N-hydroxysuccinimide) ester (NHSMA) in Trifluoroethanol (TFE) and carrying out polymerization reaction to obtain a PSBMA-b-PNHSMA block polymer;
s3, MoS in S1 2 Reacting the nanosheets and dopamine hydrochloride (DA-HCl) in a Tris-HCl buffer solution to obtain MoS 2 @ PDA nano sheet;
s4, MoS in S3 2 The @ PDA nano sheet and the PSBMA-b-PNHSMA block polymer in the S2 react in a PBS buffer solution to prepare the zwitterionic polymer modified nano sheet MoS2@ PDA @ PSBMA.
Preferably, in S2, sulfobetaine methacrylate (SBMA), Azobisisobutyronitrile (AIBN) and 4-cyano-4- (thiobenzoyl) pentanoic acid (CPAD) are dissolved in Trifluoroethanol (TFE) to obtain PSBMA macrocta, a macroinitiator, by polymerization.
Preferably, the sulfobetaine methacrylate (SBMA), Azobisisobutyronitrile (AIBN) and 4-cyano-4- (thiobenzoyl) pentanoic acid (CPAD) are in a molar ratio of (200-20): 2: 1.
Further preferably, the molar ratio of the macroinitiator PSBMA macroCTA and methacrylic acid (N-hydroxysuccinimide) ester (NHSMA) in S2 is (10-1): 1.
more preferably, MoS in S3 2 The reaction concentration of the nano-sheets is 1 mg/mL; said dopaThe reaction concentration of amine hydrochloride (DA-HCl) was 1mg/mL or 2 mg/mL.
More preferably, MoS in S4 2 The reaction concentration of the @ PDA nano-sheet is 1 mg/mL; the reaction concentration of the PSBMA-b-PNHSMA block polymer is 1 mg/mL-10 mg/mL; the PBS buffer concentration was 0.1M, pH 8.0.
Still more preferably, the reaction conditions in S3 and S4 are temperature 40 ℃ and time 24 h.
The invention also provides the zwitterionic polymer modified nanosheet MoS2@ PDA @ PSBMA prepared by the preparation method and application thereof in osteoarthritis treatment.
The invention has the following beneficial effects:
firstly) the invention prepares MoS by an ultrasonic method 2 Nano-sheets, and then carrying out dopamine modification on the nano-sheets; preparing a block PSBMA-b-PNHSMA copolymer by a RAFT method; the PSBMA-b-PNHSMA segmented polymer and the nano sheet modified by dopamine are prepared by a one-pot method to obtain a nano sheet MoS2@ PDA @ PSBMA modified by a zwitterionic polymer. In the preparation process, various functional components can be mutually promoted, so that the biological function can be better exerted, the prepared MoS2@ PDA @ PSBMA nano-sheet can eliminate over-expressed active oxygen in an inflammatory environment, the oxidation resistance is strong, the nano-sheet is not easily influenced by degrading enzyme, and the lubricating property is excellent to protect articular cartilage;
and secondly), the preparation process is simple, the reaction condition is mild, special equipment is not needed, and the method is suitable for industrial production and application and popularization.
Drawings
FIG. 1 shows MoS in example 1 2 (1-A) and MoS 2 Scanning Electron micrographs of @ PDA (1-B);
FIG. 2 is an IR spectrum of a PSBMA-b-PNHSMA block polymer of example 1;
FIG. 3 shows MoS in example 1 2 A transmission electron micrograph of @ PDA @ PSBMA (MPP) nanosheet;
FIG. 4 shows MoS in example 4 2 Graph of @ PDA @ PSBMA (MPP) nano-sheet catalyzing hydrogen peroxide to generate oxygen;
FIG. 5 shows MoS in example 5 2 A test result graph of the cleaning performance of @ PDA @ PSBMA (MPP) nano-sheets to hydroxyl free radicals (. OH);
FIG. 6 shows MoS in examples 6 and 7 2 A test result graph of the @ PDA @ PSBMA (MPP) nanosheet on scavenging performance of hydroxyl radicals and superoxide anions in cells;
FIG. 7 shows MoS in example 8 2 Graph of results of a cytocompatibility test of @ PDA @ PSBMA (MPP) nanosheets;
FIG. 8 shows MoS in example 9 2 A lubricating performance test result graph of @ PDA @ PSBMA (MPP) nanosheets;
FIG. 9 shows MoS in example 10 2 And @ PDA @ PSBMA (MPP) nanosheet photothermal conversion performance experimental result graph.
Detailed Description
The invention is further described with reference to the following figures and specific embodiments.
Example 1
1. Preparation of MoS by ultrasonic method 2 Nano-sheet
1.5g of MoS are weighed 2 Dissolving the powder in 500mL of mixed solvent of ethanol and water (volume ratio of ethanol to water 113: 137), performing ultrasonic treatment for 12 hours, and centrifuging at low speed to remove MoS which is not made into nanosheets 2 And then centrifuging at high speed for 20 minutes, and discarding the supernatant to collect the nanosheets. Placing the obtained nano-sheets in a vacuum oven for 24 hours, and further removing the solvent to obtain MoS 2 Nanosheets. MoS 2 The scanning electron microscope image of the nanosheet is shown in figure 1-A.
2. Preparation of PSBMA-b-PNHSMA Block Polymer
Sulfobetaine methacrylate (SBMA, 18mmol), azobisisobutyronitrile (AIBN, 0.18mmol) and the chain transfer agent 4-cyano-4- (thiobenzoyl) pentanoic acid (CPAD, 0.09mmol) were dissolved in 5mL Trifluoroethanol (TFE) and oxygen in the reaction system was removed by triple freezing. Under the condition of keeping out of the light, the reaction tube is placed into an oil bath pot (65 ℃) to be polymerized for 24 hours, and the ice methanol is precipitated for 3 times to obtain the macromolecular initiator PSBMA macro CTA.
Dissolving macroinitiators PSBMA macro CTA, AIBN (0.03mmol) and methacrylic acid (N-hydroxysuccinimide) ester (NHSMA, the molar charge ratio of the macroinitiators is 1: 10) in 4mL of TFE, removing oxygen in a reaction system by triple freezing, reacting in an oil bath at 65 ℃ in a dark place for 24 hours, and precipitating for 3 times by using ice methanol after the polymerization is finished to obtain a product PSBMA-b-PNHSMA block polymer. The IR spectrum of the PSBMA-b-PNHSMA block polymer is shown in FIG. 2.
3. Preparation of MoS 2 @ PDA (MP) nano-sheet
The MoS prepared in the step 1 is added 2 Dissolving the nanosheet and dopamine hydrochloride (DA & HCl) in Tris-HCl buffer solution to obtain MoS 2 The concentration of the nano-sheet is 1mg/mL, and the concentration of dissolved DA & HCl is 1 mg/mL. Reacting at 40 ℃ for 24 hours, centrifuging, washing with deionized water for 3 times, and vacuum drying to obtain MoS 2 @ PDA (MP) nanoplate. MoS 2 Scanning electron micrographs of @ PDA (MP) nanosheets are shown in FIG. 1-B.
4. Preparation of MoS 2 @ PDA @ PSBMA (MPP) nanosheet
The MoS prepared in the step 3 is added 2 @ PDA nanosheet and PSBMA-b-PNHSMA block copolymer prepared in step 2 were dissolved in PBS buffer (0.1M, pH 8.0), and MoS was added 2 The concentration of the dissolved @ PDA nano-sheet is 1 mg/mL; the concentration of the dissolved PSBMA-b-PNHSMA block copolymer is respectively 10mg/mL, after the reaction is carried out for 24 hours at the temperature of 40 ℃, the solution is washed for 3 times by deionized water after centrifugation, and the MoS is obtained by vacuum drying 2 @ PDA @ PSBMA (MPP) nanoplatelets. MoS 2 FIG. 3 shows a SEM image of @ PDA @ PSBMA (MPP) nanosheets.
Example 2
1. Preparation of MoS by ultrasonic method 2 Nano-sheet
1.5g of MoS are weighed 2 Dissolving the powder in 500mL of mixed solvent of ethanol and water (volume ratio of ethanol to water 113: 137), performing ultrasonic treatment for 12 hours, and centrifuging at low speed to remove MoS which is not made into nanosheets 2 And then centrifuging at high speed for 20 minutes, and discarding the supernatant to collect the nanosheets. Placing the obtained nanosheets in a vacuum oven for 24 hours, and further removing the solvent to obtain MoS 2 Nanosheets.
2. Preparation of PSBMA-b-PNHSMA Block Polymer
Sulfobetaine methacrylate (SBMA, 9mmol), azobisisobutyronitrile (AIBN, 0.18mmol) and the chain transfer agent 4-cyano-4- (thiobenzoyl) pentanoic acid (CPAD, 0.09mmol) were dissolved in 5mL Trifluoroethanol (TFE) and oxygen in the reaction system was removed by triple freezing. Under the condition of keeping out of the light, the reaction tube is placed into an oil bath pot (65 ℃) to be polymerized for 24 hours, and the ice methanol is precipitated for 3 times to obtain the macromolecular initiator PSBMA macro CTA.
Dissolving macroinitiators PSBMA macro CTA, AIBN (0.03mmol) and methacrylic acid (N-hydroxysuccinimide) ester (NHSMA, the molar charge ratio of the macroinitiators is 1: 5) in 4mL TFE, removing oxygen in a reaction system through triple freezing, reacting in an oil bath at 65 ℃ in a dark place for 24 hours, and precipitating for 3 times by using ice methanol after polymerization to obtain a product PSBMA-b-PNHSMA block polymer.
3. Preparation of MoS 2 @ PDA (MP) nano-sheet
The MoS prepared in the step 1 is added 2 Dissolving the nanosheet and dopamine hydrochloride (DA & HCl) in Tris-HCl buffer solution to obtain MoS 2 The concentration of the nano-sheet is 1mg/mL, and the concentration of dissolved DA & HCl is 2 mg/mL. Reacting at 40 ℃ for 24 hours, centrifuging, washing with deionized water for 3 times, and vacuum drying to obtain MoS 2 @ PDA (MP) nanoplate.
4. Preparation of MoS 2 @ PDA @ PSBMA (MPP) nanosheet
The MoS prepared in the step 3 is added 2 The @ PDA nano-sheet and the PSBMA-b-PNHSMA block copolymer prepared in step 2 were dissolved in PBS buffer (0.1M, pH 8.0), and MoS 2 The concentration of the dissolved @ PDA nano-sheet is 1 mg/mL; the dissolved concentration of the PSBMA-b-PNHSMA block copolymer was 5 mg/mL at 40 ℃ After 24 hours of reaction, the mixture is centrifuged, washed by deionized water for 3 times and dried in vacuum to obtain MoS 2 @ PDA @ PSBMA (MPP) nanoplatelets.
Example 3
1. Preparation of MoS by ultrasonic method 2 Nano-sheet
1.5g of MoS are weighed 2 Dissolving the powder in 500mL of mixed solvent of ethanol and water (volume ratio of ethanol to water 113: 137), performing ultrasonic treatment for 12 hours, and centrifuging at low speed to remove MoS which is not made into nanosheets 2 And then centrifuging at high speed for 20 minutes, and discarding the supernatant to collect the nanosheets. Placing the obtained nano-sheets in a vacuum oven for 24 hours, and further removing the solvent to obtain MoS 2 Nanosheets.
2. Preparation of PSBMA-b-PNHSMA Block Polymer
Sulfobetaine methacrylate (SBMA, 1.8mmol), azobisisobutyronitrile (AIBN, 0.18mmol) and the chain transfer agent 4-cyano-4- (thiobenzoyl) pentanoic acid (CPAD, 0.09mmol) were dissolved in 5mL Trifluoroethanol (TFE), and oxygen in the reaction system was removed by triple freezing. Under the condition of keeping out of the light, the reaction tube is placed into an oil bath pot (65 ℃) to be polymerized for 24 hours, and the ice methanol is precipitated for 3 times to obtain the macromolecular initiator PSBMA macro CTA.
Dissolving macroinitiators PSBMA macro CTA, AIBN (0.03mmol) and methacrylic acid (N-hydroxysuccinimide) ester (NHSMA, the molar charge ratio of the macroinitiators is 1: 1) in 4mL of TFE, removing oxygen in a reaction system by triple freezing, reacting in an oil bath at 65 ℃ in a dark place for 24 hours, and precipitating for 3 times by using ice methanol after polymerization to obtain a product PSBMA-b-PNHSMA block polymer.
3. Preparation of MoS 2 @ PDA (MP) nano-sheet
The MoS prepared in the step 1 is added 2 Dissolving the nanosheet and dopamine hydrochloride (DA & HCl) in Tris-HCl buffer solution to obtain MoS 2 The concentration of the nanosheet is 1mg/mL, and the concentration of dissolved DA & HCl is 1 mg/mL. Reacting at 40 ℃ for 24 hours, centrifuging, washing with deionized water for 3 times, and vacuum drying to obtain MoS 2 @ PDA (MP) nanoplate.
4. Preparation of MoS 2 @ PDA @ PSBMA (MPP) nanosheet
The MoS prepared in the step 3 is added 2 The @ PDA nano-sheet and the PSBMA-b-PNHSMA block copolymer prepared in step 2 were dissolved in PBS buffer (0.1M, pH 8.0), and MoS 2 The concentrations of the dissolved @ PDA nano sheet and the dissolved PSBMA-b-PNHSMA block copolymer are both 1 mg/mL; after 24 hours of reaction at 40 ℃, centrifugation, deionized water washing for 3 times and vacuum drying are carried out to obtain MoS 2 @ PDA @ PSBMA (MPP) nanoplatelets.
Example 4 MoS 2 @ PDA @ PSBMA (MPP) nanosheet catalysisHydrogen peroxide (H) 2 O 2 ) Test for generating oxygen
Adding H in normal saline 2 O 2 The solution was diluted to 1.0 mmol/L. MoS prepared in example 1 2 @ PDA @ PSBMA (MPP) nanosheet and control material MoS thereof 2 (M) nanosheet, MoS 2 @ PDA (MP) nanosheets were dispersed in physiological saline at a concentration of 200. mu.g/mL. It is reacted with H 2 O 2 The solutions were mixed in equal volumes and incubated in a 37 ℃ incubator for 2 hours. The incubated solution was taken out and the resulting oxygen bubbles were recorded by photography. Experimental results as shown in fig. 4, the MPP nanosheet co-incubated solution generated more oxygen bubbles.
Example 5 MoS 2 Test for scavenging of hydroxyl radical (. OH) by @ PDA @ PSBMA (MPP) nanosheet
MoS prepared in example 1 at various concentrations 2 @ PDA @ PSBMA (MPP) nanosheet and control material MoS thereof 2 (M) nanosheet, MoS 2 @ PDA (MP) nano-sheet dispersed in 200 mu M H 2 O 2 In the solution, a terephthalic acid solution with a concentration of 1.0mM is added according to the equal volume, and the mixture is placed in a constant temperature water bath at 37 ℃ in a dark place for incubation for 12 hours, and then fluorescence spectrum scanning is carried out. The excitation wavelength was set to 312nm and the scanning range of the emission wavelength was set to 350-600 mn. The test result is shown in figure 5, compared with M and MP nano-sheets, the MPP nano-sheet has better OH removing effect, and the removing efficiency can reach 93.42% under the concentration of 100 mug/mL.
Example 6 MoS 2 Test for detecting scavenging performance of @ PDA @ PSBMA (MPP) nanosheet on hydroxyl free radicals in cells
RAW 264.7 cells were seeded at a density of 50000 cells/well in 24-well plates. After the cells were completely adherent, the cells were stimulated with a medium containing lipopolysaccharide (LPS, 1. mu.g/mL) for 12 hours, and then continued with a medium containing MoS prepared in example 1 2 @ PDA @ PSBMA (MPP) nanosheet and control material MoS thereof 2 (M) nanosheet, MoS 2 The culture medium of @ PDA (MP) nanosheets was continued for 24 hours. And finally, labeling by a DCFH-DA fluorescent probe and observing the expression condition of hydroxyl radicals in the cells by a fluorescence microscope. Test results are as followsFigure 6 shows that under the induction of LPS, hydroxyl free radicals generated in cells are obviously increased, and when the nano-sheets are added into a culture medium, the hydroxyl free radicals expressed in the cells are eliminated, wherein the elimination effect of the MPP nano-sheets is the best.
Example 7 MoS 2 Test for detecting scavenging performance of @ PDA @ PSBMA (MPP) nanosheet on intracellular superoxide anions
This example is essentially identical to example 6, except that the cells were labeled with DHE fluorescent probe and observed for intracellular superoxide anion expression.
The experimental results are shown in figure 6, under the induction of LPS, the superoxide anion produced in the cells is obviously increased, and MoS 2 The nano-sheet has no superoxide anion scavenging performance, and MP and MPP can scavenge hydroxyl radicals expressed in cells, wherein the scavenging effect of the MPP nano-sheet is the best.
Example 8 MoS 2 Cytocompatibility of @ PDA @ PSBMA (MPP) nanosheet
The cells selected in this example were rat chondrocytes. First, rat chondrocytes were cultured to logarithmic growth phase in DMEM/F12 medium containing 10% FBS and 1% double antibody. Next, chondrocytes were seeded in 96-well plates at a density of 5000 cells/well. After the cells are completely attached to the wall, the cell culture medium is replaced by a culture medium containing MPP nano sheets with different concentrations (the MPP nano sheets are prepared in example 1), CCK-8 reagent is added into each hole after 1 day of culture, and after continuous culture is carried out for 3 hours, the absorbance at 450nm is tested by using an enzyme labeling instrument. The experimental result is shown in figure 7, and the cell survival rate is over 80% under the co-culture of the low-concentration MPP nano-sheets, so that good cell compatibility is reflected.
Example 9 MoS 2 Test for detecting lubricating property of @ PDA @ PSBMA (MPP) nanosheet
The base material selected in the embodiment is a sheet cut from bovine cartilage, the test frequencies are 1Hz, 3Hz and 5Hz respectively, and the test time is 10 min; the pressures were set to 1N, 2N and 5N, respectively. The lubricating properties of the different nanosheet solutions were compared. The experimental results are shown in FIG. 8, comparing MPP to MoS 2 And MP nanosheet, with better lubricationPerformance, its coefficient of friction can be as low as 0.05.
Example 10 MoS 2 Photothermal conversion test of @ PDA @ PSBMA (MPP) nanosheet
MoS prepared in example 1 2 @ PDA @ PSBMA (MPP) nanosheet is configured into a nanosheet solution of 0.1mg/mL, and then a 808 laser is set with lasers (0.2, 0.5 and 1.0W-cm) of different powers -2 ) The solution was irradiated and the temperature change recorded using an infrared thermal imager. The test results are shown in FIG. 9, and the MPP has the light-heat conversion performance of 1W/cm 2 Under the laser power, the temperature can be raised to 40 ℃, and the temperature is favorable for treating arthritis.
In conclusion, the test data of the embodiments 4 to 10 fully show that the MoS2@ PDA @ PSBMA nanosheet prepared by the present invention can remove the active oxygen overexpressed in the inflammatory environment, has strong oxidation resistance, good cell compatibility, excellent lubricating property and photothermal conversion property, can effectively protect the articular cartilage, and is suitable for the treatment of arthritis.
The present specification and figures are to be regarded as illustrative rather than restrictive, and it is intended that all such alterations and modifications that fall within the true spirit and scope of the invention, and that all such modifications and variations are included within the scope of the invention as determined by the appended claims without the use of inventive faculty.

Claims (9)

1. A preparation method of a zwitterionic polymer modified nanosheet is characterized by comprising the following steps:
s1, taking molybdenum disulfide (MoS) 2 ) Dissolving the powder in mixed solvent of ethanol and water, and ultrasonically preparing MoS 2 Nanosheets;
s2, dissolving macroinitiator PSBMA macro CTA, Azodiisobutyronitrile (AIBN) and methacrylic acid (N-hydroxysuccinimide) ester (NHSMA) in Trifluoroethanol (TFE) and carrying out polymerization reaction to obtain PSBMA-b-PNHSMA block polymer;
s3, MoS in S1 2 Reacting the nanosheets and dopamine hydrochloride (DA-HCl) in a Tris-HCl buffer solution to obtain MoS2@ PDA nanosheets;
S4、MoS in S3 2 And reacting the @ PDA nanosheet with the PSBMA-b-PNHSMA block polymer in the S2 in a PBS buffer solution to prepare the zwitter-ion polymer modified nanosheet MoS2@ PDA @ PSBMA.
2. The method of claim 1, wherein sulfobetaine methacrylate (SBMA), Azobisisobutyronitrile (AIBN) and 4-cyano-4- (thiobenzoyl) pentanoic acid (CPAD) are dissolved in Trifluoroethanol (TFE) in S2 to obtain PSBMA macrocta by polymerization.
3. The method according to claim 2, wherein the sulfobetaine methacrylate (SBMA), Azobisisobutyronitrile (AIBN), and 4-cyano-4- (thiobenzoyl) pentanoic acid (CPAD) are present in a molar ratio of (200-20): 2: 1.
4. The preparation method of claim 1, wherein the molar ratio of the macroinitiator PSBMA macroCTA and (N-hydroxysuccinimide) methacrylate (NHSMA) in S2 is (10-1): 1.
5. the method according to claim 1, wherein MoS in S3 2 The reaction concentration of the nano-sheets is 1 mg/mL; the reaction concentration of the dopamine hydrochloride (DA-HCl) is 1mg/mL or 2 mg/mL.
6. The method according to claim 1, wherein MoS in S4 2 The reaction concentration of the @ PDA nano-sheet is 1 mg/mL; the reaction concentration of the PSBMA-b-PNHSMA block polymer is 1 mg/mL-10 mg/mL; the PBS buffer concentration was 0.1M, pH 8.0.
7. The method of claim 1, wherein the reaction conditions in S3 and S4 are a temperature of 40 ℃ and a time of 24 h.
8. As claimed in any one of claims 1 to 7The nano-sheet MoS modified by the zwitterionic polymer prepared by the preparation method 2 @PDA@PSBMA。
9. Zwitterionic polymer-modified nanosheet MoS of claim 8 2 Use of @ PDA @ PSBMA in the treatment of osteoarthritis.
CN202210597817.6A 2022-05-30 2022-05-30 Preparation method and application of amphoteric ion polymer modified nanosheet Pending CN114984309A (en)

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