CN114982770B - 一种防治芝麻枯萎病的组合物及应用 - Google Patents
一种防治芝麻枯萎病的组合物及应用 Download PDFInfo
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Abstract
本发明提供了一种防治芝麻枯萎病的组合物及应用,属于作物病害防治技术领域。本发明防治芝麻枯萎病的组合物,有效成分为中生菌素和蛇床子素,所述中生菌素和蛇床子素的质量比为4:1‑1:4。中生菌素和蛇床子素联用,具有增效作用;两种有效成分均为生物源农药,对人畜、环境无害;两种有效成分抑菌机制不同,致使芝麻枯萎病不易产生抗药性,便于应用推广。
Description
技术领域
本发明属于作物病害防治技术领域,尤其涉及一种防治芝麻枯萎病的组合物及应用。
背景技术
芝麻,隶属于胡麻科胡麻属,是世界上最古老的油料作物之一,也是我国重要的优质油料作物。我国芝麻年种植面积1200万亩左右,总产量约75万吨,在国际油料贸易中具有重要的地位(张海洋,2007)。近几年,芝麻主产区枯菱病等病害、渍害的发生导致我国芝麻产量不稳,年份间产量差异较大进而影响到我国芝麻种植、加工和贸易等产业的稳定和正常发展。
芝麻枯萎病,主要在我国河南、湖北、江西等主要产区发生较重,其他地区也有零星发生。芝麻枯萎病是一种维管束病害,整株表现症状。幼苗受害根部腐烂、枯死;成株受害时,叶片自下而上逐渐变黄枯萎,最后变褐枯死,有整株枯死的,也有仅茎杆半边枝叶枯死。茎上病斑褐色、长条形,潮湿时病斑上出现粉红色霉层,即病菌的分生孢子。横切病茎,可见维管束变褐色。病株上蒴果早熟而裂,种子瘦瘪。
针对于芝麻枯萎病,当前主要有种植抗病品种,病田轮作等。而采用药剂防治时,则使用大量化学药剂,如多菌灵等,容易对环境造成污染,致使芝麻残留大量农药制剂成分。中生菌素、蛇床子素作为生物源农药,具有环保、绿色、无残留的优点,但联合应用于芝麻枯萎病防治未有报道。
发明内容
有鉴于此,本发明的目的在于提供一种防治芝麻枯萎病的组合物及应用,通过中生菌素和蛇床子素按质量比为4:1-1:4联用,有效抑制芝麻枯萎病病原菌繁殖生长,降低芝麻枯萎病发病率。
为了实现上述发明目的,本发明提供了以下技术方案:
一种防治芝麻枯萎病的组合物,有效成分为中生菌素和蛇床子素,所述中生菌素和蛇床子素的质量比为4:1-1:4。
优选的是,所述中生菌素和蛇床子素的质量比为1:1。
优选的是,所述有效成分中生菌素和蛇床子素在所述组合物中占重量比为8-12%,其余为辅料。
更优选的是,所述有效成分中生菌素和蛇床子素在所述组合物中占重量比为10%。
优选的是,所述组合物剂型包括可湿性粉剂、粉剂、可湿性粉剂、乳油、悬浮剂、浓乳剂、缓释剂。
本发明还提供了一种防治芝麻枯萎病的组合物在防治芝麻枯萎病中的应用。
优选的是,将8-12%中生菌素·蛇床子素组合物稀释成400-800倍液,喷施防治芝麻枯萎病。
更优选的是,所述喷施量为:喷施至芝麻叶片呈水珠但不滴下。
相对于现有技术,本发明具有如下有益效果:
本发明提供了一种防治芝麻枯萎病的组合物,以中生菌素和蛇床子素联合应用作为有效成分。
通过室内毒力测定与复配比例筛选,当中生菌素和蛇床子素按质量比1:4-4:1配比时,具有明显的增效作用,其中中生菌素:蛇床子素=1:1增效作用最为明显,对芝麻枯萎病防治效果达到88%以上,优于中生菌素、蛇床子素单剂防治效果。
中生菌素和蛇床子素均为生物源农药,对人畜毒性低,对环境、人体健康均未产生负面影响。
此外,中生菌素与蛇床子素的抑菌机制不同,两者联用,芝麻枯萎病病原菌不易产生抗药性,便于应用推广,长时间应用。
具体实施方式
本发明提供了一种防治芝麻枯萎病的组合物,有效成分为中生菌素和蛇床子素,所述中生菌素和蛇床子素的质量比为4:1-1:4。
中生菌素,广谱农用杀菌抗生素。通过抑制细菌的菌体蛋白质合成和使真菌菌丝畸形,从而抑制孢子萌发和杀死孢子而致效,对革兰氏阳性和阴性菌、分支杆菌、酵母菌及真菌有效。其被用于防治水稻白叶枯病、大白菜软腐病和柑橘溃疡病等。本发明将其应用于芝麻枯萎病的防治,亦产生了明显防效。
蛇床子素,蛇床子素具有农药活性基因—异戊烯结构,表现为独特的杀虫抑菌活性。研究表明蛇床子素不仅对菜青虫、小菜蛾、蚜虫等害虫和夜蛾卵块有作用,而且对植物病原真菌如黄瓜白粉病菌、葡萄霜霉病菌,辣椒疫霉病菌、小麦赤霉病菌等都具有显著的抑制作用。本发明将其应用于芝麻枯萎病的防治,亦产生了明显防效。
本发明优选中生菌素和蛇床子素的质量比为2:1-1:2;更优选中生菌素和蛇床子素的质量比为1:1。
本发明优选有效成分中生菌素和蛇床子素在所述组合物中占重量比为8-12%,其余为辅料;进一步优选有效成分在组合物中占重量比为10%。
本发明优选所述组合物剂型包括可湿性粉剂、粉剂、可湿性粉剂、乳油、悬浮剂、浓乳剂、缓释剂。本发明对上述剂型的辅料没有特殊限定,根据各剂型需要选择本领域常用辅料即可。
本发明还提供了一种防治芝麻枯萎病的组合物在防治芝麻枯萎病中的应用。
本发明优选将8-12%中生菌素·蛇床子素组合物稀释成400-800倍液,喷施防治芝麻枯萎病;进一步优选稀释倍数为500-700倍;更优选为600倍。
本发明优选防治芝麻枯萎病时,所述药剂喷施量为:喷施至芝麻叶片呈水珠但不滴下。
下面结合实施例对本发明提供的技术方案进行详细的说明,但是不能把它们理解为对本发明保护范围的限定。
实施例1
一种防治芝麻枯萎病的组合物,有效成分中生菌素5%,蛇床子素5%。
经上述组合物用水稀释600倍,喷施至芝麻植株叶面呈水滴但不滴下,以防治芝麻枯萎病。
实施例2
一种防治芝麻枯萎病的组合物,有效成分中生菌素4%,蛇床子素4%。
经上述组合物用水稀释400倍,喷施至芝麻植株叶面呈水滴但不滴下,以防治芝麻枯萎病。
实施例3
一种防治芝麻枯萎病的组合物,有效成分中生菌素6%,蛇床子素6%。
经上述组合物用水稀释800倍,喷施至芝麻植株叶面呈水滴但不滴下,以防治芝麻枯萎病。
实施例4
一种防治芝麻枯萎病的组合物,有效成分中生菌素8%,蛇床子素2%。
经上述组合物用水稀释600倍,喷施至芝麻植株叶面呈水滴但不滴下,以防治芝麻枯萎病。
实施例5
一种防治芝麻枯萎病的组合物,有效成分中生菌素4%,蛇床子素8%。
经上述组合物用水稀释700倍,喷施至芝麻植株叶面呈水滴但不滴下,以防治芝麻枯萎病。
实施例6
一种防治芝麻枯萎病的组合物,有效成分中生菌素2.4%,蛇床子素9.6%。
经上述组合物用水稀释500倍,喷施至芝麻植株叶面呈水滴但不滴下,以防治芝麻枯萎病。
实施例7
室内毒力测定与复配比例筛选
1.供试药剂
3%中生菌素可湿性粉剂和1%蛇床子素乳油。
2.供试菌株
分离于江西省农业科学院芝麻种植试验田,经鉴定,为尖孢镰刀菌芝麻专化型(Fusarium oxysporum Schi.F.sp.sesami(Zap.)Cast),4℃冰箱内保存备用。
3.1实验方法
采用FAO推荐的菌体生长速率法。
表1中生菌素和蛇床子素复配比例
表2中生菌素和蛇床子素稀释浓度
3.2含药平板的制备
按照3.1设定的药剂浓度梯度,将药剂加入定量溶化的PDA培养基中,制成含药平板,以不含药平板为空白对照,每浓度重复3次。
3.3接菌培养
用直径6mm打孔器沿培养好的菌落边缘打取菌盘,用消毒接种针将菌盘反转移植到平板上,置于23℃条件下恒温培养7天。
3.4结果调查
以直尺交叉测量法测量菌落扩展直径,与不施药对照菌盘扩展直径相比,计算出药剂对菌丝生长的抑制率。
抑制率(%)=(对照菌落扩展直径-处理菌落扩展直径)/对照菌落扩展直径×100
4.统计分析
根据各药剂浓度对数值及对应的菌丝生长抑制率几率值作回归分析,计算各药剂毒力回归方程、相关系数(r)和EC50。进行药剂联合毒力测定时,根据Wadley法计算复配组合物的增效系数(SR),评价复配组合物的联合作用类型。根据增效系数(SR)来评价药剂复配的增效作用,即SR<0.5为拮抗作用,0.5≤SR≤1.5为相加作用,SR>1.5为增效作用。增效系数(SR)按以下公式计算:
式中:
X1:复配组合物EC50理论值,单位为mg/L;
PA:复配组合物中A的百分含量,单位为百分率(%);
PB:复配组合物中B的百分含量,单位为百分率(%);
A:A的EC50值,单位为mg/L;
B:B的EC50值,单位为mg/L。
式中:
SR:复配组合物的增效系数;
X1:复配组合物EC50理论值,单位为mg/L;
X2:复配组合物EC50实测值,单位为mg/L。
5.结果分析
通过室内毒力实验,表3结果表明两种单剂室内对芝麻枯萎病病原菌均表现出良好的抑制效果,EC50分别为3.3631mg/L和4.0255mg/L。中生菌素和蛇床子素两者有效成分比例为1:4-4:1时均表现出增效作用,尤其1:1配比增效最为显著,其EC50为0.9615mg/L,增效系数为3.42。说明,中生菌素·蛇床子素1:1复配杀菌剂对芝麻枯萎病具有很高的杀菌活性。
表3中生菌素与蛇床子素最佳复配比例筛选
实施例8
中生菌素与蛇床子素最佳复配比例室内防治效果试验
取种植于蛭石与土壤按2:1混合的灭菌基质上生长的健康芝麻幼苗(5×5=25穴)。设置中生菌素药剂、蛇床子素药剂、中生菌素·蛇床子素1:1复配药剂和CK共4个处理,如表4,每个处理3个重复。
表4各药剂有效成分含量及稀释倍数
药剂 | 有效成分含量 | 稀释倍数 |
CK | —— | —— |
中生菌素药剂 | 3% | 360倍 |
蛇床子素药剂 | 1% | 120倍 |
中生菌素·蛇床子素1:1复配药剂 | 5%中生菌素:5%蛇床子素 | 600倍 |
幼苗缓苗后以每个育苗盘(100株)喷200ml病原菌孢子悬浮液(106CFU/ml)于芝麻幼苗上。1d后分别喷洒上述药剂及水(CK)。在喷施后,每3天观察发病情况,3周后统计发病率及防治效果。杀菌剂对病害的防治效果按公式计算。
防治效果=(对照区CK发病率-处理区发病率)/对照区CK发病率×100%
通过室内防治效果试验,表5试验结果表明两种单剂室内对芝麻枯萎病病原菌均表现出良好的抑制效果,防效分别为67.4%和60.5%。在有效成分喷适量相同的情况下,中生菌素和蛇床子素两者有效成分比例为1:1时防效更加明显,发病率仅有9.4%,防治效果达到88.6%。说明,中生菌素·蛇床子素1:1复配杀菌剂对芝麻枯萎病具有很高的杀菌活性。
表5中生菌素与蛇床子素最佳复配比例对芝麻枯萎病防治效果
以上所述仅是本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。
Claims (7)
1.一种防治芝麻枯萎病的组合物,其特征在于,有效成分为中生菌素和蛇床子素,所述中生菌素和蛇床子素的质量比为4:1-1:4;所述有效成分中生菌素和蛇床子素在所述组合物中占重量比为8-12%,其余为辅料。
2.根据权利要求1所述的防治芝麻枯萎病的组合物,其特征在于,所述中生菌素和蛇床子素的质量比为1:1。
3.根据权利要求1所述的防治芝麻枯萎病的组合物,其特征在于,所述有效成分中生菌素和蛇床子素在所述组合物中占重量比为10%。
4.根据权利要求1-3任一项所述的防治芝麻枯萎病的组合物,其特征在于,所述组合物剂型为可湿性粉剂、粉剂、乳油、悬浮剂、浓乳剂、缓释剂中的任意一种。
5.权利要求1-3所述任一项防治芝麻枯萎病的组合物在防治芝麻枯萎病中的应用。
6.根据权利要求5所述的应用,其特征在于,将8-12%中生菌素·蛇床子素组合物稀释成400-800倍液,喷施防治芝麻枯萎病。
7.根据权利要求6所述的应用,其特征在于,所述喷施量为:喷施至芝麻叶片呈水珠但不滴下。
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