CN114982754B - Methyl heptenone black beard fly attractant, preparation method and application thereof - Google Patents
Methyl heptenone black beard fly attractant, preparation method and application thereof Download PDFInfo
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- CN114982754B CN114982754B CN202210670689.3A CN202210670689A CN114982754B CN 114982754 B CN114982754 B CN 114982754B CN 202210670689 A CN202210670689 A CN 202210670689A CN 114982754 B CN114982754 B CN 114982754B
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- JPTOCTSNXXKSSN-UHFFFAOYSA-N methylheptenone Chemical compound CCCC=CC(=O)CC JPTOCTSNXXKSSN-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 239000005667 attractant Substances 0.000 title claims abstract description 10
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 10
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 241000282828 Camelus bactrianus Species 0.000 claims abstract description 12
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical group CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 14
- 239000002904 solvent Substances 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 230000002265 prevention Effects 0.000 claims description 2
- 230000006698 induction Effects 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 18
- 238000002474 experimental method Methods 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 abstract description 4
- 206010046914 Vaginal infection Diseases 0.000 abstract description 3
- 230000004044 response Effects 0.000 description 17
- 241000589902 Leptospira Species 0.000 description 15
- 230000006399 behavior Effects 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 241001599018 Melanogaster Species 0.000 description 9
- 230000000638 stimulation Effects 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 241000282836 Camelus dromedarius Species 0.000 description 4
- 241000255925 Diptera Species 0.000 description 4
- 208000006123 Myiasis Diseases 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 241001425390 Aphis fabae Species 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 241001116415 Balanophora Species 0.000 description 2
- 241000255601 Drosophila melanogaster Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 206010061217 Infestation Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000256103 Simuliidae Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 230000000472 traumatic effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 201000002481 Myositis Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000257185 Sarcophagidae Species 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 241000609108 Wohlfahrtia Species 0.000 description 1
- 208000010608 Wound myiasis Diseases 0.000 description 1
- 210000003790 arthropod antennae Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 244000309465 heifer Species 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 210000003905 vulva Anatomy 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Toxicology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a methylheptenone black beard fly attractant, a preparation method and application thereof. The invention determines the feeler potential and behavior reaction of the Bactrian camel vaginal volatile substance-methyl heptenone by using a gas chromatography-feeler potential combination technology (GC-EAD), a feeler potential technology (EAG) and a behavior experiment, and knows the influence of methyl heptenone on the attractive behavior of the Bactrian camel. Experiments prove that the methyl heptenone used by the invention can attract the black hair fly, thereby reducing the contact opportunity of the black hair fly and the Bactrian camel and effectively preventing and controlling the colpitis caused by the black hair fly.
Description
Technical Field
The invention belongs to the technical field of biological prevention and control, and particularly relates to a methylheptenone black beard fly attractant, a preparation method and application thereof.
Background
The fly is belonging to Diptera (Diptera), rotifidae (Sarcophagidae), dermata (Subfamily Paramacronychinae), dermata (Genus Wohlfahrtia Brauer), and is an important pathogen causing traumatic myiasis of human and livestock. The Bactrian camel vaginalis is the only pathogen causing Bactrian camel vaginosis and is widely distributed in areas such as south Europe, middle east, north Africa and Xinjiang and inner Mongolia of China. The extent of infestation by the black fly varies from region to region, depending on the composition of the parasite flora, the host, animal husbandry and control practices, climate and geography-related factors. There are related reports in europe that the infection rate of sheep infested with black hair fly is as high as 90%. However, bactrian camels are the main infestations of the black flies in other areas. In one report of iran, it was found that there is about 60% of traumatic diseases caused by larvae of the black hair fly. On both farms in north morocco, dogs were examined for traumatic myositis and all larvae and adults found and cultivated from them were identified as black hair flies. It has been reported that still other animals are also infected with the larvae of the fly larvae of the black hair. In addition, the larvae of the fly, which is a black hair, can cause various diseases of human beings, such as diseases of ears, eyes, vulva, skin and the like.
In China, the black hair fly mainly causes vaginal myiasis of the mother camel, seriously affects the growth and development performance of the diseased camel and the development of the camel industry, and becomes a primary hazard disease of the mother camel. At present, the control of myiasis mainly based on pesticides and drug expelling treatments, except killing fly maggots at parasitic positions, the myiasis can not play a role in the environment, and after the drug effects, the myiasis can be continuously produced and repeatedly infected. In order to change the current situation of pesticide-based control, biological control measures mainly comprising the black hair fly in artificial interference environment are needed to be explored.
Disclosure of Invention
The invention aims to solve the existing technical problems and provides a methylheptenone black hair fly attractant which can attract adult black hair fly to reduce the contact opportunity with a host, thereby influencing mating and maggot production behaviors and providing a new thought for treating Bactrian camel vaginal fly maggot disease.
The invention is realized by the following technical scheme:
the methylheptenone black beard fly attractant is prepared by the following steps:
methyl heptenone is dissolved in a solvent.
In the above technical scheme, the solvent is n-hexane.
In the technical scheme, the concentration of the methyl heptenone is 10 -4 Mu g/mu L to 1 mu g/mu L.
The preparation method of the methylheptenone black beard fly attractant comprises the following steps:
methyl heptenone is dissolved in a solvent.
In the above technical scheme, the solvent is n-hexane.
In the technical scheme, the concentration of the methyl heptenone is 10 -4 Mu g/mu L to 1 mu g/mu L.
The application of the methylheptenone black beard fly attractant is as follows: the methyl heptenone attracts the black hair fly, reduces the contact opportunity of the black hair fly and the Bactrian camel, and prevents and controls the colpitis caused by the black hair fly.
The beneficial effects of the invention are as follows:
the invention determines the feeler potential and behavior reaction of the Bactrian camel vaginal volatile substance-methyl heptenone by using a gas chromatography-feeler potential combination technology (GC-EAD), a feeler potential technology (EAG) and a behavior experiment, and knows the influence of methyl heptenone on the attractive behavior of the Bactrian camel. Experiments prove that the methyl heptenone used by the invention can attract the black hair fly, thereby reducing the contact opportunity of the black hair fly and the Bactrian camel and effectively preventing and controlling the colpitis caused by the black hair fly.
Drawings
FIG. 1 shows the GC-EAD reaction of 1 day old Balanophora nigra on methyl heptenone.
FIG. 2 shows the GC-EAD reaction of 7 day old Balanophora nigra on methyl heptenone.
FIG. 3 shows EAG response of male Leptospira melanogaster to various concentrations of methyl heptenone.
FIG. 4 shows EAG response of female Leptospira melanogaster to various concentrations of methyl heptenone.
FIG. 5 is a comparison of EAG reactions of Drosophila melanogaster to methyl heptenone.
FIG. 6 shows the behavioral response of male Leptospira melanogaster to methyl heptenone.
FIG. 7 shows the behavioral response of female Leptospira melanogaster to methyl heptenone.
FIG. 8 is a comparison of the behavioral response of fly Leptospira nigricans to methyl heptenone.
Other relevant drawings may be made by those of ordinary skill in the art from the above figures without undue burden.
Detailed Description
In order to make the actual technical means, the creation characteristics, the achievement of the purposes and the effects of the present invention easy to understand, the technical scheme of the present invention is further described below with reference to the specific embodiments.
Examples
The electrophysiological and behavioral response of methyl heptenone to the fly of the black hair comprises the following steps:
1. GC-EAD reaction of Leptospira nigrum on methyl heptenone
(1) Sample preparation: the purchased methylheptenone (. Gtoreq.97%) was formulated as 10 using HPLC grade n-hexane -2 Mu g/. Mu.L of sample to be tested.
(2) Preparing a glass capillary tube: (1) automatically preparing by using an electrode drawing instrument; (2) and under the alcohol lamp, the drawing is carried out manually, and the drawing process is kept balanced as much as possible. The drawn capillary tip can be just as well placed into the insect antenna. After the preparation of the glass capillary tube, 0.9% physiological saline is filled.
(3) Preparing and connecting antennae: the tentacle of the black hair fly is sheared off from the base by using an ophthalmic surgical scissors under a microscope, the tentacle is adsorbed into the capillary by using the surface tension of physiological saline in the capillary, the tentacle is lapped on two glass electrodes of an tentacle potentiometer, the base of the tentacle is connected with a reference electrode, and the top of the tentacle is connected with a recording electrode. After the EAD baseline is basically stable, sample injection is started, and an electrode is recorded. The recorded signals are amplified and converted by an IDAC-2 signal collector, and then data acquisition and analysis are carried out by GC-EAD software. Selecting black hair fly of about 1 day old and 7 days old, and measuring 10 pairs -2 The GC-EAD reaction of mu g/. Mu.L methyl heptenone was repeated 3 times for each sample, once for each antenna.
(4) GC conditions: the initial temperature is 120℃for 1min, and the temperature is raised to 280℃at a rate of 20℃per min for 15 min. Split sample injection is carried out at a ratio of 5:1, and 1 mu L of sample is manually injected each time.
2. EAG reaction of Leptospira nigrum on methyl heptenone
(1) Sample preparation: preparation of methyl heptenone with chromatography grade n-hexane to 10 -4 μg/μL、10 -3 μg/μL、10 -2 μg/μL、10 -1 The samples to be tested of μg/μl and 1 μg/μl were stored in a-20deg.C refrigerator for use.
(2) Selecting adult black beard fly of about 5-7 days old, cutting off the antenna from the base, and connecting the antenna. Taking 10 mu L of sample to be tested, uniformly dripping the sample on a filter paper strip with the thickness of 30mm multiplied by 10mm, and putting the sample into a clean 1mL blue gunIn the head, after volatilizing the solvent for about 5min, stimulation was performed. The EAG reaction values of the samples were corrected by blank EAG measurement with n-hexane (HPLC grade) as a blank control, before and after each antenna EAG measurement. Samples were tested in a random fashion and the assay was performed at room temperature. The tail end of the dropper is connected with a stimulation gas control device, the stimulation time is 0.2s each time, the stimulation interval is 60s, the stimulation airflow speed is set to 300mL/min, and the continuous airflow is set to 1000mL/min. Each sample was repeated 6 times to distinguish between male and female. The EAG stimulation sequence for each sample was: blank Control (CK) →10 -4 μg/μL→10 -3 μg/μL→10 -2 μg/μL→10 -1 Mu g/. Mu.L → 1. Mu.g/. Mu.L → blank Control (CK).
The EAG relative reaction value is calculated as follows:
sample antenna potential response relative value = 2× (antenna potential response value for test sample)/(antenna potential response value for solvent control before test sample + antenna potential response value for solvent control after test sample)
(3) Statistical analysis: the EAG response data were collated using Excel 16.0, analyzed and plotted using GraphPad prism8.0.1, and both the same gender EAG response to different test samples and the different gender EAG response to the same test sample were analyzed using One-Way ANOVA (P < 0.05).
3. Behavioral response of fly Leptospira nigricans to methyl heptenone
(1) The device is sequentially connected with an atmospheric sampling instrument (air outlet), a drying tower filled with active carbon, a wetting device, an odor bottle and a Y-shaped test tube by using a water purifier tube, and the air flow of two side arms is maintained at 1L/min. And selecting black hair fly of about 5-7 days old, and performing behavior experiment measurement. Only one black hair fly is placed at the straight arm opening of the Y-shaped test tube each time, the reaction time is 5min, and when the insect enters more than 1/2 of the side arm and stays for at least 30s as a selection result, and recording is carried out. If no selection is made within 5 minutes, the behavioral determination of the insect is stopped and no record is made. The female and male worms are respectively recorded for 60, 3 groups are repeated, each group is 20, each worm with the same concentration is tested only once, each test is tested for 5 worms, the Y-shaped test tube is horizontally rotated for 180 degrees, and the positions of the two arms are exchanged, so that errors are reduced. After testing 10 insects, cleaning the inner arms of the Y-shaped tube with 75% alcohol and distilled water respectively, putting the inner arms into a drying box for drying, and replacing a clean Y-shaped test tube. The experiment was continued. The black hair fly active period is mainly carried out in the daytime, so the test time is 9:00-17:00, the whole experiment was performed at room temperature and fasted for 12 hours before the experiment. The test was also performed from low to high concentration, with n-hexane as a blank.
(2) And (3) statistics: behavioral response data were collated using Excel 16.0, and the black fly was analyzed for 3 compounds using GraphPad prism8.0.1 with t-test and plotted using Origin PRO.
Analysis of results
As can be seen from FIGS. 1 and 2, the male and female adult pair 10 of Drosophila melanogaster of different ages -2 Mu.g/. Mu.L of methylheptenone were all GC-EAD reacted.
The EAG relative response values of male Leptospira melanogaster to various concentrations of methyl heptenone are shown in FIG. 3. Analysis shows that the EAG relative reaction values of male black fly to methyl heptenone with different concentrations are different, and the EAG relative reaction value is increased firstly and then decreased and then increased along with the increase of the methyl heptenone concentration, and the relative reaction value reaches the maximum when the concentration is maximum. As the concentration increases, the relative response value of EAG changes less and less. 10 -4 μg/μL、10 -3 μg/μL、10 -2 μg/μL、10 -1 EAG relative reaction values of. Mu.g/. Mu.L and 1. Mu.g/. Mu.L were 1.167mV, 1.313mV, 1.688mV, 1.654mV, 1.803mV, respectively. The EAG relative response values of male Leptospira melanogaster caused by different concentrations of methyl heptenone were not significantly different (P > 0.05).
The EAG relative response values of female Leptospira to various concentrations of methyl heptenone are shown in FIG. 4. The results show that female black flies have certain EAG reactions on methyl heptenone with different concentrations, and the relative reaction value changes with the change of the concentration. The EAG relative response value of female Leptospira melanogaster increased with increasing methyl heptenone concentration. At a concentration of 1. Mu.g/. Mu.L, the EAG relative reaction value was 2.138mv, significantly higher than 10 -4 Mu g/. Mu.L and 10 -3 EAG relative response value (P < 0.05), 10 in μg/μl concentration -4 μg/μL、10 -3 μg/μL、10 -2 Mu g/. Mu.L and 10 -1 The relative reaction values of μg/μl EAG were 1.157mv, 1.263mv, 1.284 mv, 1.841mv, respectively, with the remainder not significant (P > 0.05).
The EAG response pair of different sexes of Leptospira nigrum to methyl heptenone is shown in FIG. 5. Analysis shows that the EAG relative response values of female and male adults to methyl heptenone are not significantly different (P > 0.05).
The results of the behavioral response of male Leptospira melanogaster to methyl heptenone (FIG. 6), 10 -2 Mu g/mu L has obvious attractiveness (P is less than 0.05) to male black hair fly; when the concentration is 10 -1 Mu g/mu L and 1 mu g/mu L, has very obvious attraction to male black hair flyMale Heifer did not respond significantly to low concentrations of methyl heptenone (P > 0.05).
The results of the behavioral response of female Leptospira melanogaster to methyl heptenone (FIG. 7), 10 -2 μg/μL、10 -1 Mu g/mu L and 1 mu g/mu L of methyl heptenone have extremely remarkable attractiveness (P is less than 0.01) to female black hair fly. Female black hair fly did not respond significantly to other concentrations of methyl heptenone (P > 0.05).
Experiments on the behavior of fly with different sexes on methyl heptenone (FIG. 8) show that when the concentration is 10 -1 At mug/mug, the attractiveness to female black hair fly is significantly higher than that to male (P < 0.05); at a concentration of 1 μg/μl, the attractiveness to females is very pronounced relative to male black-hair flies. The rest concentrations were not significantly different (P > 0.05).
The foregoing has described exemplary embodiments of the invention, it being understood that any simple variations, modifications, or other equivalent arrangements which would not unduly obscure the invention may be made by those skilled in the art without departing from the spirit of the invention.
Claims (1)
1. The application of the methylheptenone black beard fly attractant is characterized in that: induction of black hair by methyl heptenoneThe fly is polluted, the contact opportunity of the black hair fly and the Bactrian camel is reduced, so that the biological prevention and control effect on the vaginosis caused by the black hair fly is achieved, and the used attractant is prepared by the following method: dissolving methyl heptenone in a solvent, wherein the solvent is n-hexane, and the concentration of the methyl heptenone is 10 -2 Mu g/mu L to 1 mu g/mu L.
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| CN109042663A (en) * | 2018-09-10 | 2018-12-21 | 柳州市华博有害生物防治有限公司 | Fruit fly trapping bait and its application |
| CN110720460A (en) * | 2019-11-12 | 2020-01-24 | 山西农业大学 | Plant source pollution-free active substance for attracting green gram, attractant and method |
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| CN109042663A (en) * | 2018-09-10 | 2018-12-21 | 柳州市华博有害生物防治有限公司 | Fruit fly trapping bait and its application |
| CN110720460A (en) * | 2019-11-12 | 2020-01-24 | 山西农业大学 | Plant source pollution-free active substance for attracting green gram, attractant and method |
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| Title |
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| "铜绿丽金龟对植物源挥发物的触角电位和行为反应";谢明惠等;《植物保护》(第1期);33-38 * |
| M. A. BIRKETT et.al."The role of volatile semiochemicals in mediating host location and selection by nuisance and disease-transmitting cattle flies".《Medical and Veterinary Entomology》.2004,(第18期),313-322. * |
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