CN1149722A

CN1149722A - Multiple liquid quality control pig serum and preparing method

Info

Publication number: CN1149722A
Application number: CN 95117435
Authority: CN
Inventors: 祁进龙; 赵海春; 吴健翎; 郑家齐
Original assignee: Individual
Current assignee: Individual
Priority date: 1995-11-09
Filing date: 1995-11-09
Publication date: 1997-05-14
Anticipated expiration: 2015-11-09
Also published as: CN1054205C

Abstract

The present invention uses fresh pig's blood as raw material and the pig serum is made up through separating, freezing, component regulating and adding glycol as stabilizer. The invented product contains multiple components for quality control of serum and overcomes the error for subpackage and redissolving of frozen dried serum.

Description

Multiple liquid quality control pig serum and preparation method

The technical field of the invention is the medical test quality control, specifically multiple liquid quality control pig serum and preparation method.

The employed quality controling serum overwhelming majority is the freeze-drying porcine blood serum on the present domestic medical test.This serum exists and redissolves error when the packing sum of errors uses before the inevitable freeze-drying.Stable inadequately to some enzyme preservation, but also exist production equipment costliness, shortcomings such as complex process.The composition of present employed freeze-drying porcine blood serum of while is ten remainders only, can not satisfy the needs of medical test development far away.

The objective of the invention is to replace present freeze-dry blood serum for society provides multiple liquid Quality Control porcine blood serum.

Technical scheme of the present invention is, uses wide material sources, and the higher pig blood of enzyme content is raw material, adds various required compositions.Make the multiple liquid Quality Control porcine blood serum that meets the Quality Control requirement with analyzing pure ethylene glycol used as stabilizers and antiseptic.

Concrete technology is:

1, gathers pig blood;

2, separation of serum;

3, each Quality Control component content in the serum analysis;

4 adjustment are respectively analyzed composition and are made into quality controlled serum;

5, filtration, packing, label.

The multiple liquid quality control pig serum for preparing contains following composition and content:

GLU1-30mmol/L, BUN2-40mmol/L, CRE50-1000 μ mol/L, UA50-2000 μ mol/L, TBIL5-3000 μ mol/L, CHO2-60mmol/L, Ca (Cacl ₂, 2H ₂O) 1-5mmol/L, P (NaH ₂PO ₄, 2H ₂O) 0.5-3mmol/L, Mg (Mgcl ₂, 6H ₂O) 0.3-5mmol/L, CL (Nacl) 50-150mmol/L, K (Kcl) 2-10mmol/L, Na (CH ₃CooNa) 50-200mmol/L, Co ₂(NaHco ₃) 10-50mmol/L, TG0.2-5mmol/L, GOT20-1000U/L, GPT20-1000U/L, LDH50-1500U/L, HBDH50-1500U/L, CK50-1500U/L, AMY50-2000U/L, AKP30-1000U/L, γ-GT20-1000U/L, ALB20-80g/L, TP20-100g/L, DBIL1-50umol/L, Fe2-50 μ mol/L, bile acid 3-50umol/L, IgG4-40g/L, IgA0.3-6g/L, IgM0.2-5g/L, C ₃0.1-3g/L, C ₄0.02-1g/L, APOA ₁0.5-5g/L, APOB0.5-5g/L, HDL-C0.5-13mmol/L, lithium 1-5mmol/L, other blood medicines of theophylline 50-500 μ mol/L: 10.

Advantage of the present invention is:

1, compares with the freeze-dry blood serum of present use, do not have the error of packing and redissolution.

2, owing to contain stabilizing agent in the serum, various analysis ingredient stabilities especially are easy to the enzyme that changes in the serum.

3, production technology is simple, does not need expensive specialized equipment.

Embodiment:

With preparation intermediate value multiple liquid quality control pig serum is example.

1, gathers pig blood: collect 5000 milliliters of free of contamination fresh pig blood in the slaughterhouse, treat to transport the laboratory back after the natural coagulation.

2, separation of serum: collect and solidified pig blood supernatant, separate, discarded the type composition through hydro-extractor.The serum of separator well is reserved detection sample postposition-30 ℃ low temperature refrigerator 24 hours, take out the room temperature fusing, freeze repeatedly molten twice.

3, each Quality Control component content in the serum analysis: analytical approach is measured the content of each Quality Control composition in the serum routinely.

4, adjusting is respectively analyzed composition and is made into the multiple liquid quality controling serum: according to the quality controlled serum requirement, on the basis of former each composition of serum, add respective substance, make it to reach requirement concentration, add the stabilizing agent-ethylene glycol (analyzing pure) of quality controlled serum total amount 1/3rd, make satisfactory quality controlled serum.

It is as follows to add step:

(1) adding of enzyme:

Enzyme content is very high in the porcine blood serum, does not generally need to add in addition the enzyme composition, only just need add the enzyme composition when preparation high value quality controlled serum.Can prepare various enzymes voluntarily, just can add after slightly carrying, needn't purifying.Wherein, GOT and GPT can extract from Pigs Hearts; It is the easiest that AMY adds people's saliva; CK can extract from muscle; AKP can extract from people's placenta; γ-GT can extract from the pig kidney.The said extracted technology is known technology.

(2) adding of albumen:

From unnecessary porcine blood serum, extract albuminous and globulin respectively with the sulfate of ammoniac salting out method, join in the quality controlled serum.

(3) adding of other compositions:

Enzyme and the adjusted serum of albumen are remeasured volume, unnecessary part is sopped up with the dried glue of polyacrylamide that contains propionyl acid 70g/L.According to the content that determines other compositions, calculate the amount that needs adding, join in the serum one by one.Wherein:

The adding method of a, TBIL (cholerythrin): the cholerythrin powder after will weighing is poured the band glass stopper in vitro, adds 0.1MNaOH5.5 milliliter vibration dissolving, mixes with serum immediately, pours in the total serum then.

The adding method of b, UA (uric acid): the uric acid after will weighing adds in 10 ml waters of 60 ℃ of pre-temperature, adds IMNaOH0.7ml, and the limit edged is shake, and this lysate is added in the total serum immediately,

(4) adding of ethylene glycol:

In above-mentioned 1 liter of serum, add 0.5 liter and analyze pure ethylene glycol, be mixed, just obtain 1.5 liters of quality controlled serums.

(5) filtration, packing, label:

The quality controlled serum that the prepares filter membrane device with 0.2 μ m is filtered, be divided in then in 10 milliliters the brown vial, adorn 3 milliliters for every.Post label.

The intermediate value multiple liquid quality control pig serum for preparing contains following composition and content: GLU5.6mmol/L, 3UN6.0mmol/L, CRE110 μ mol/L, UA400 μ mol/L, TBIL34 μ mol/L, CHO6.0mmol/L, Ca (Cacl ₂2H ₂O) 2.5mmol/L, P1.5mmol/L, Mg1.0mmol/L, CL105mmol/L, K5.0mmol/L, Na (CH ₃CooNa) 140mmol/L, Co ₂(NaHCO ₃28mmol/L, TG0.54mmol/L, GOT132u/L, GPT52u/L, LDH750u/L, HBDH552u/L, CK373u/L, AMY832u/L, AKP56u/L, γ-GT30u/L, ALB45g/L, TP70g/L, DBIL6umol/L, Fe25 μ mol/L, bile acid 10umol/L, IgG8g/L, IgA1g/L, IgM1g/L, C ₃1g/L, C ₄0.2g/L, APOA ₁1.5g/L, APOB1.0g/L, HDL-C1.5mmol/L, lithium 2.0mmol/L, other blood medicines of theophylline 110 μ mol/L: 10.

Claims

1, a kind of multiple liquid quality control pig serum, it is characterized by: it contains ethylene glycol, also contains routine composition and content down.

GLU1-30mmol/L, BUN2-40mmol/L, CRE50-1000 μ mol/L, UA50-2000 μ mol/L, TBIL5-3000 μ mol/L, CHO2-60mmol/L, Ca1-5mmol/L, P0.5-3mmol/L, Mg0.3-5mmol/L, CL50-150mmol/L, K2-10mmol/L, Na50-200mmol/L, Co ₂10-50mmol/L, TG0.2-5mmol/L, GOT20-1000U/L, GPT20-1000U/L, LDH50-1500U/L, HBDH50-1500U/L, CK50-1500U/L, AMY50-2000U/L, AKP30-1000U/L, γ-GT20-1000U/L, ALB20-80g/L, TP20-100g/L, DBIL1-50umol/L, Fe2-50 μ mol/L, bile acid 3-50umol/L, IgG4-40g/L, IgA0.3-6g/L, IgM0.2-5g/L, C ₃0.1-3g/L, C ₄0.02-1g/L, APOA ₁0.5-5g/L, APOB0.5-5g/L, HDL-C0.5-13mmol/L, lithium 1-5mmol/L, other blood medicines of theophylline 50-500 μ mol/L: 10.

2, a kind of multiple liquid quality control pig serum as claimed in claim 1 is characterized by, and glycol content is 1/3rd of a quality controlled serum total amount.

3, a kind of multiple liquid quality control pig serum as claimed in claim 1 is characterized by, and the content of each composition of intermediate value multiple liquid quality controling serum is:

GLU5.6mmol/L, BUN6.0mmol/L, CRE110 μ mol/L, UA400 μ mol/L, TBIL34 μ mol/L, CBO6.0mmol/L, Ca2.5mmol/L, P1.5mmol/L, Mg1.0mmol/L, CL105mmol/L, K5.0mmol/L, Na140mmol/L, Co ₂28mmol/L, TG0.54mmol/L, GOT132u/L, GPT52u/L, LDH750u/L, HBDH552u/L, CK373u/L, AMY832u/L, AKP56u/L, γ-GT30u/L, ALB45g/L, TP70g/L, DBIL6umol/L, Fe25 μ mol/L, bile acid 10umol/L, IgG8g/L, IgAlg/L, IgMlg/L, C ₃Lg/L, C ₄0.2g/L, APOA ₁1.5g/L, APOB1.0g/L, HDL-C1.5mmol/L, lithium 2.0mmol/L, other blood medicines of theophylline 110 μ mol/L: 10.

4, a kind of method for preparing the multiple liquid quality controling serum comprises and gathers pig blood, precipitation, separation, freezing, dissolving, it is characterized by, and needs to add ethylene glycol and each special quality control composition in preparation.