CN114966079A

CN114966079A - Automatic biochemical immunoassay device and method

Info

Publication number: CN114966079A
Application number: CN202210446374.0A
Authority: CN
Inventors: 朱海; 袁旭军; 王振煜
Original assignee: Suzhou Yike Xinchuang Scientific Instrument Co ltd
Current assignee: Suzhou Yike Xinchuang Scientific Instrument Co ltd
Priority date: 2022-04-26
Filing date: 2022-04-26
Publication date: 2022-08-30
Also published as: WO2023207115A1

Abstract

The invention provides an automatic biochemical immunoassay device and a detection method, comprising a basic detection unit, wherein the basic detection unit comprises a flow path module, a valve assembly, a push rod assembly and a driving assembly. The valve assembly is connected with the flow path module and is used for controlling the on-off of the flow path; the push rod assembly is connected with the flow path module and is used for driving liquid in the flow path module to flow; the driving assembly is connected with the valve assembly and the push rod assembly and used for controlling the on-off of the valve assembly and the movement of the push rod assembly. The invention can realize the automatic quantitative distribution of the sample microliter liquid, and realizes the automatic preparation and analysis of the biochemical immunoreaction liquid and improves the analysis and detection efficiency by presetting different biochemical reaction reagents or immunoassay reagents in the chamber.

Description

Automatic biochemical immunoassay device and method

Technical Field

The invention relates to the technical field of automatic quantitative liquid adding, in particular to an automatic biochemical immunoassay device and an immunoassay method.

Background

The biochemical immunoassay method has wide clinical detection application in various markers, and has more and more detectable items in sex hormone, thyroid hormone, tumor markers, metabolic substances, infectious disease markers, cardiovascular and cerebrovascular disease markers and the like. The biochemical immunoassay method has the advantages of sensitive detection method, good target specificity, wide linear range of various analysis target concentrations and the like.

At present, biochemical immunoassay widely applied in clinic is mostly detected in a manual or semi-automatic mode, and used equipment is mostly a discrete biochemical analyzer which comprises a sample tray, a reagent rack, a sample adding device, a reagent adding device, a reaction tray, a detector, a reaction device cleaning device, a stirring device and other main parts. The detection devices need a large amount of manual operation intervention, but the manual operation has uncertainty such as individual difference, manual error and irregular operation, and the defects are revealed under the condition of multiple sample numbers and test projects.

In order to solve the problems, a few companies, such as Beckmann Coulter biochemical immune flow line, Roche Cobas biochemical immune flow line and the like, have introduced biochemical immune flow lines at home and abroad, and the essence of the systems is that a plurality of full-automatic analyzers are connected together through a sample pipe conveying system, so that the whole system is complex and expensive, is only applied to a few large hospitals at present, and is not convenient to popularize to primary medical institutions and families.

Therefore, there is a strong need in the market for a biochemical immunoassay device that can be used in basic medical institutions and homes for testing, and further, it is required to improve the degree of automation and miniaturize it for easy movement and use.

Disclosure of Invention

The invention provides an automatic biochemical immunoassay device and a detection method, which are used for solving the problems of low sample distribution efficiency, large equipment, difficult automatic quantification of trace liquid and the like in the prior art and realizing the automatic quantitative distribution of microliter liquid of a sample.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

according to a first aspect of the present application, the present invention provides an automated biochemical immunoassay device comprising a basic detection unit including a flow path module, a valve assembly, a push rod assembly, and a driving assembly.

The valve assembly is connected with the flow path module and is used for controlling the on-off of the flow path; the push rod assembly is connected with the flow path module and is used for driving liquid in the flow path module to flow; the driving assembly is connected with the valve assembly and the push rod assembly and used for controlling the on-off of the valve assembly and the movement of the push rod assembly.

Further, the flow path module comprises a liquid inlet cavity, an inflow channel, a quantitative cavity, an outflow channel and a liquid storage detection cavity, the liquid inlet cavity is used for storing liquid to be detected, the inflow channel is connected with the liquid inlet cavity and the quantitative cavity, and the outflow channel is connected with the quantitative cavity and the liquid storage detection cavity.

Further, the liquid inlet chamber is located higher than the quantitative chamber in the gravity direction, so that the liquid to be detected can flow into the quantitative chamber from the liquid inlet chamber by utilizing the gravity.

Further, the flow path module is made of a material which is corrosion resistant and has good chemical stability, and preferably, the flow path module is made of one or a combination of a plurality of materials selected from Polycarbonate (PC), Polystyrene (PS), polypropylene (PP), polymethyl methacrylate (PMMA), polylactic acid (PLA), acrylonitrile-butadiene-styrene (ABS), photosensitive resin, carbon steel, stainless steel, aluminum and copper.

Further, the valve assembly comprises a fluid inlet valve and a fluid outlet valve, wherein the fluid inlet valve is arranged on the inflow channel and used for controlling the on-off of the inflow channel; the fluid push-out valve is arranged on the outflow channel and used for controlling the on-off of the outflow channel.

Preferably, the valve cores of the fluid introducing valve and the fluid pushing-out valve are both rotary valve cores, and the rotary valve cores have fluid microstructures, so that the transferred fluid is more stable.

Further, the junction of the fluid push-out valve and the outflow channel is set to be in a necking shape, so that the quantified sample is discharged from the outflow channel to the liquid storage detection chamber through the fluid discharge port.

Further, the push rod assembly comprises a rod body, a first limiting plate, a second limiting plate, a sealing element, a locking nut and a return spring; the two end parts of the rod body are provided with the first limiting plate and the second limiting plate, the first limiting plate extends into the quantitative cavity, and the second limiting plate is located outside the quantitative cavity.

Further, the sealing element is fixedly connected with the first limiting plate; preferably, the first limiting plate is matched with a screw nut to fasten the sealing element at one end of the rod body. The sealing member cooperates with an inner wall of the dosing chamber to divide the dosing chamber into two portions which are not in communication with each other. The sealing element is made of soft sealing material, preferably, the sealing element is made of one of silica gel, rubber or PP.

Furthermore, a push-out positioning block and a push-in positioning block are arranged on the inner wall of the quantitative cavity, and the rod body moves between the push-out positioning block and the push-in positioning block. The push-out positioning block and the push-in positioning block can limit the movement of the rod body within a certain range, so that the volume of fluid in the quantitative cavity can be ensured, and the rod body can be prevented from being separated from the quantitative cavity to cause the fluid to overflow from the quantitative cavity.

Furthermore, the pushing positioning block is detachably connected with the quantitative cavity, and the pushing positioning block is detached when the rod body and the first limiting plate are rotated into the quantitative cavity.

Furthermore, a position feedback sensor is arranged at one end, extending into the quantitative cavity, of the rod body, and the position feedback sensor is used for feeding back the propelling stroke of the first driving piece to the rod body. The position feedback sensor can realize the sensing and distance detection of the liquid sample under the non-contact condition. The position feedback sensor is connected with the driving assembly and used for feeding back position information of the push rod assembly.

Preferably, the position feedback sensor is one of a sliding resistor, a time-of-flight (ToF) laser sensor, a high precision ultrasonic sensor, an image sensor with an optical system, and an image sensor without an optical system.

Further, when an image sensor with an optical system is selected and the image sensor without the optical system is used as the position feedback sensor, a light source is arranged in the quantitative cavity and used for illuminating the quantitative cavity.

Further, when a sliding resistor is selected, an electrode is arranged on the first limiting plate, a resistance device which can be communicated with the electrode is arranged on the side wall of the quantitative chamber, the electrode is in conductive connection with the resistance period, and preferably, the resistance period is made of a precise resistor.

Further, the driving assembly comprises a first driving device and a second driving device, and the first driving device is connected with the second limiting plate and used for driving the push rod assembly to move, so that quantitative distribution of the liquid to be detected is completed.

Specifically, the first driving member may be an electric cylinder, an air cylinder or other external power device to precisely control the position of the quantitative push rod, so that the quantitative push rod may move within a specified range to control the volume of the fluid in the quantitative chamber.

The second driving device comprises a first motor and a second motor, and the first motor is connected with the fluid introducing valve and used for driving the fluid introducing valve to rotate between on-off states; the second motor is connected with the fluid push-out valve and used for driving the fluid push-out valve to rotate between on-off.

Specifically, the first motor and the second motor may be one of a stepping motor, a dc brushless motor, a servo motor, or a lead screw motor.

Further, automatic biochemical immunodetection device can include more than two sets of basic detecting element, a feed liquor cavity is used in the basic detecting element sharing of multiunit, also the multiunit flows into the inflow passageway and communicates with same feed liquor cavity promptly.

Further, any reagent related to biochemical analysis or immunoassay can be preset in any chamber or flow channel of the device, including but not limited to sample lysis solution, enzyme, coenzyme, antibody, substrate, indicator, color reagent, protective agent, blocking solution, reaction stopping solution, etc.

According to a second aspect of the application, an automated biochemical immunoassay device detection method comprises the following steps:

s1, before the quantitative operation is performed by using the immunoassay device, the first motor is controlled to drive the fluid introducing valve to the off state, and the second motor is controlled to drive the fluid ejecting valve to the on state.

S2, controlling the first driving piece to drive the push rod assembly to move into the quantitative cavity and discharging air in the quantitative cavity; and when the rod body moves to the pushing positioning block, controlling the first driving part to stop driving the push rod assembly.

And S3, controlling the first motor to drive the fluid introducing valve to be in a conducting state, and controlling the second motor to drive the fluid ejecting valve to be in a closing state.

S4, controlling the first driving piece to drive the push rod assembly to move out of the quantitative cavity, and enabling the liquid to be detected to flow into the quantitative cavity from the liquid inlet cavity under the action of gravity; when the rod body moves to the pushing positioning block or the preset position of the position feedback sensor, the first driving part is controlled to stop driving the push rod assembly.

And S5, controlling the first motor to drive the fluid introducing valve to be in a closed state, and controlling the second motor to drive the fluid ejecting valve to be in a conducting state.

S6, controlling the first driving piece to drive the push rod assembly to move towards the quantitative cavity, so that the liquid to be detected in the quantitative cavity is driven to be discharged into the liquid storage detection cavity through the fluid push-out valve and the outflow channel; and when the rod body moves to the pushing positioning block, controlling the first driving part to stop driving the push rod assembly.

The automatic biochemical immunoassay device and the detection method provided by the invention realize the automatic quantitative distribution of the liquid sample, and can greatly improve the distribution efficiency of the sample; and through setting up multiunit basic detection unit, can once realize the multiunit ration to waiting to detect liquid, follow-up can carry out different detections to quantitative sample, promoted the efficiency of the automatic ration of liquid sample and the efficiency of follow-up detection greatly.

Drawings

Fig. 1 is a schematic layout of a basic detection unit of the present invention.

Fig. 2 is a schematic view of another basic detection unit arrangement of the present invention.

FIG. 3 is a schematic view of a driving assembly of the present invention.

Fig. 4 is a schematic diagram of the working process of the present invention.

FIG. 5 is a schematic diagram of the basic detection unit arrangement of the present invention with additional reagent chambers.

Fig. 6 is a flowchart of a detection method according to a second embodiment of the invention.

Fig. 7 is a flowchart of a detection method according to a fourth embodiment of the present invention.

Fig. 8 is a flowchart of a detection method according to a seventh embodiment of the present invention.

Reference numerals: 1. a basic detection unit; 10. a flow path module; 11. a liquid inlet chamber; 12. an inflow channel; 13. a dosing chamber; 14. an outflow channel; 15. a reservoir detection chamber; 20. a valve assembly; 21. a fluid introduction valve; 22. a fluid push-out valve; 30. a push rod assembly; 31. a rod body; 32. a first limit plate; 33. a second limiting plate; 34. a seal member; 35. locking the nut; 36. a return spring; 37. a position feedback sensor; 371. a light source; 372. an electrode; 40. a drive assembly; 41. a first driving device; 42. a second driving device; 421. a first motor; 422. a second motor; 50. a dosing chamber; 51. pushing out the positioning block; 52. pushing the positioning block.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example one

Referring to fig. 1-3, an embodiment of the present invention is shown: an automatic biochemical immunoassay device includes a basic test unit 1, the basic test unit 1 includes a flow path module 10, a valve assembly 20, a push rod assembly 30 and a driving assembly 40. The valve assembly 20 is connected with the flow path module 10 and is used for controlling the on-off of the flow path; the push rod assembly 30 is connected to the flow path module 10 and is used for driving the liquid in the flow path module 10 to flow; the driving assembly 40 is connected to the valve assembly 20 and the push rod assembly 30, and is configured to control on/off of the valve assembly 20 and movement of the push rod assembly 30.

In this embodiment, the flow path module 10 includes an inlet chamber 11, an inflow channel 12, a quantifying chamber 5013, an outflow channel 14 and a reservoir detection chamber 15, the inlet chamber 11 is used for storing a liquid to be detected, the inflow channel 12 is connected to the inlet chamber 11 and the quantifying chamber 5013, and the outflow channel 14 is connected to the quantifying chamber 5013 and the reservoir detection chamber 15.

Further, the liquid inlet chamber 11 is located higher than the quantifying chamber 5013 in the gravity direction, so that the liquid to be detected can flow into the quantifying chamber 5013 from the liquid inlet chamber 11 by using gravity.

In addition, the flow path module 10 is made of a material with corrosion resistance and good chemical stability, and preferably, the flow path module 10 is made of one or a combination of a plurality of materials selected from Polycarbonate (PC), Polystyrene (PS), polypropylene (PP), polymethyl methacrylate (PMMA), polylactic acid (PLA), acrylonitrile-butadiene-styrene (ABS), photosensitive resin, carbon steel, stainless steel, aluminum, and copper.

In the present embodiment, the valve assembly 20 includes a fluid inlet valve 21 and a fluid outlet valve 22, the fluid inlet valve 21 is disposed on the inflow channel 12 and is used for controlling the on-off of the inflow channel 12; the fluid push-out valve 22 is arranged on the outflow channel 14 and used for controlling the on-off of the outflow channel 14.

Preferably, the spools of the fluid inlet valve 21 and the fluid outlet valve 22 are both rotary spools, and the rotary spools have fluid microstructures to make the transferred fluid more stable.

Further, the junction of the fluid push-out valve 22 and the outflow channel 14 is set to be in a constricted shape, so that the quantified sample is discharged from the outflow channel 14 to the reservoir detection chamber 15 through the fluid discharge port.

In this embodiment, the push rod assembly 30 includes a rod body 31, a first limit plate 32, a second limit plate 33, a sealing member 34, a lock nut 35, and a return spring 36; the first limiting plate 32 and the second limiting plate 33 are arranged at two end portions of the rod body 31, the first limiting plate 32 extends into the quantitative cavity 5013, and the second limiting plate 33 is located outside the quantitative cavity 5013.

Further, the sealing element 34 is fixedly connected with the first limit plate 32; preferably, the first limit plate 32 is engaged with a screw nut to fasten the sealing member 34 to one end of the rod 31. The seal 34 cooperates with the inner wall of the dosing chamber 5013 to divide the dosing chamber 5013 into two parts which are not in communication with each other. The sealing member 34 is made of a soft sealing material, and preferably, the material of the sealing member 34 is one of silicone, rubber or PP.

In addition, a push-out positioning block 51 and a push-in positioning block 52 are provided on an inner wall of the metering chamber 5013, and the rod 31 is movable between the push-out positioning block 51 and the push-in positioning block 52. The push-out positioning block 51 and the push-in positioning block 52 can limit the movement of the rod 31 within a certain range, i.e., can ensure the volume of the fluid in the dosing chamber 5013, and can prevent the rod 31 from separating from the dosing chamber 5013 to cause the fluid to overflow from the dosing chamber 5013.

Further, the push-out positioning block 51 is detachably connected to the quantitative cavity 5013, and when the rod 31 and the first limiting plate 32 are rotated into the quantitative cavity 5013, the push-out positioning block 51 is detached.

In this embodiment, a position feedback sensor 37 is disposed at an end of the rod 31 extending into the metering chamber 5013, and the position feedback sensor 37 is used for feeding back the advancing stroke of the first driving member to the rod 31. The position feedback sensor 37 can perform sensing and distance detection with or without contact with the liquid sample. The position feedback sensor 37 is connected to the driving assembly 40 for feeding back the position information of the push rod assembly 40.

Preferably, the position feedback sensor 37 is one of a sliding resistor, a time-of-flight (ToF) laser sensor, a high precision ultrasonic sensor, an image sensor with an optical system, and an image sensor without an optical system.

When an image sensor with an optical system or an image sensor without an optical system is used as the position feedback sensor 37, a light source 371 is also disposed in the quantitative chamber 5013 to illuminate the quantitative chamber 5013.

When a sliding resistor is selected, an electrode 372 is arranged on the first limiting plate 32, a resistance device which can be communicated with the electrode 372 is arranged on the side wall of the quantitative cavity 5013, the electrode 372 is in conductive connection with the resistance period, and the resistance period is preferably made of a precise resistor.

The driving assembly 40 includes a first driving device 41 and a second driving device 42, and the first driving device 41 is connected to the second limiting plate 33 and is configured to drive the push rod assembly 30 to move, so as to complete the quantitative dispensing of the liquid to be detected.

Specifically, the first driving member may be an electric cylinder or an air cylinder, which is used as an external power device to precisely control the position of the quantitative push rod, so that the quantitative push rod can move within a predetermined range, thereby controlling the volume of the fluid in the quantitative chamber 5013.

The second driving device 42 includes a first motor 421 and a second motor 422, the first motor 421 is connected to the fluid introducing valve 21, and is configured to drive the fluid introducing valve 21 to rotate between on and off states; the second motor 422 is connected to the fluid push-out valve 22, and is configured to drive the fluid push-out valve 22 to rotate between on and off states.

Specifically, the first motor 421 and the second motor 422 may be one of a stepping motor, a dc brushless motor, a servo motor, or a lead screw motor.

Further, the automatic biochemical immunoassay device may include more than two sets of basic detecting units 1, and the multiple sets of basic detecting units 1 share one liquid inlet chamber 11, that is, the multiple sets of inflow channels 12 are communicated with the same liquid inlet chamber 11. So set up, can once realize the multiunit ration to waiting to detect liquid.

Example two

The detection method of the automatic biochemical immunoassay device provided based on the embodiment comprises the following steps:

and S1, controlling the first motor to drive the fluid leading-in valve to be in a closed state, and controlling the second motor to drive the fluid leading-out valve to be in a conducting state.

Specifically, before the quantitative operation is performed by using the automatic biochemical immunoassay device, the first motor 421 is controlled to drive the fluid introducing valve 21 to be in the off state, and the second motor 422 is controlled to drive the fluid ejecting valve 22 to be in the on state.

S2, controlling the first driving piece to drive the push rod assembly to move in the quantitative cavity, and controlling the first driving piece to stop driving the push rod assembly when the rod body moves to the position of the push positioning block.

Specifically, the first driving member is controlled to drive the push rod assembly 30 to move towards the quantitative chamber 5013, so as to exhaust air in the quantitative chamber 5013; when the rod body 31 moves to the push-in positioning block 52, the first driving part is controlled to stop driving the push rod assembly 30.

Specifically, the first motor 421 is controlled to drive the fluid introducing valve 21 to be in an on state, and the second motor 422 is controlled to drive the fluid ejecting valve 22 to be in an off state.

S4, controlling the first driving piece to drive the push rod assembly to move outside the quantitative cavity, and controlling the first driving piece to stop driving the push rod assembly when the rod body moves to the position where the positioning block is pushed or the position feedback sensor is preset.

Specifically, the first driving member is controlled to drive the push rod assembly 30 to move out of the quantitative chamber 5013, and at this time, the liquid to be detected flows into the quantitative chamber 5013 from the liquid inlet chamber 11 under the action of gravity; when the rod 31 moves to the position of the push-in positioning block 52 or a preset position of the position feedback sensor 37, the first driving part is controlled to stop driving the push rod assembly 30.

And S5, controlling the first motor to drive the fluid introducing valve to be in a closed state, and controlling the second motor to drive the fluid pushing-out valve to be in a conducting state.

Specifically, the first motor 421 is controlled to drive the fluid introducing valve 21 to be in the off state, and the second motor 422 is controlled to drive the fluid ejecting valve 22 to be in the on state.

S6, controlling the first driving piece to drive the push rod assembly to move in the quantitative cavity, and controlling the first driving piece to stop driving the push rod assembly when the rod body moves to the position of the push positioning block.

Specifically, the first driving member is controlled to drive the push rod assembly 30 to move towards the quantitative chamber 5013, so as to drive the liquid to be detected in the quantitative chamber 5013 to be discharged into the liquid storage detection chamber 15 through the fluid push-out valve 22 and the outflow channel 14; when the rod body 31 moves to the push-in positioning block 52, the first driving part is controlled to stop driving the push rod assembly 30.

By the detection method of the automatic biochemical immunodetection device, automatic quantitative distribution of the liquid sample can be realized, and the distribution efficiency of the sample can be greatly improved; and through setting up multiunit basic detecting element 1, can once realize the multiunit ration to waiting to detect liquid, follow-up can carry out different detections to quantitative sample, promoted the efficiency of the automatic ration of liquid sample and the efficiency of follow-up detection greatly.

EXAMPLE III

Referring to fig. 4, the present invention further provides a linkage mechanism, wherein gear structures are further disposed on the valve cores of the fluid inlet valve 21 and the fluid outlet valve 22, and a thread structure matched with the gear is disposed on the rod 31, wherein the length of the thread structure can drive the rotation angle of the valve core of the fluid inlet valve 21 and the valve core of the fluid outlet valve 22 to be greater than 90 °.

Further, the valve core of the fluid introducing valve 21 and the valve core of the fluid pushing valve 22 are further provided with a gear structure, and the matching relationship between the gear structure and the thread structure on the rod body 31 is set as follows: when the gate of the fluid introduction valve 21 is in the on state, the fluid push-out valve 22 is in the off state.

Through the arrangement of the linkage mechanism, the basic detection unit 1 can realize quantitative distribution of the liquid to be detected only by arranging one group of the first driving devices 41, so that the mechanism is simplified, the equipment cost is reduced, and the control difficulty is also reduced.

Example four

The detection method of the automatic biochemical immunoassay device based on the third embodiment comprises the following steps:

and S10, controlling the first driving device to push the push rod assembly into the quantitative chamber, so that the fluid introducing valve is driven to be in a conducting state, and the fluid pushing valve is linked to be in a closing state.

Specifically, as shown in fig. 4a), the first driving device 41 is controlled to push the push rod assembly 30 into the quantitative chamber 13, so as to drive the fluid introducing valve 21 to be in a conducting state, and to interlock the fluid ejecting valve 22 to be in a closing state.

And S20, driving the push rod assembly to move in the direction away from the quantitative chamber by the first driving device, closing the fluid introducing valve and opening the fluid pushing valve.

Specifically, as shown in fig. 4b), when the fluid introducing valve 21 is opened, the first driving device 41 is controlled to drive the push rod assembly 30 to move away from the quantitative chamber 13, during which the strip detection liquid is sucked into the quantitative chamber 5013 by the action of air pressure, and during which the fluid introducing valve 21 is closed and the fluid pushing-out valve 22 is opened.

And S30, controlling the first driving device to drive the push rod assembly to move towards the quantitative chamber.

Specifically, as shown in fig. 4c), after the above operation is completed, the first driving device 41 is controlled to drive the push rod assembly 30 to move into the quantitative chamber 5013, and at this time, the liquid in the quantitative chamber 5013 enters the liquid storage detection chamber 15 through the fluid outflow channel 14.

EXAMPLE five

Referring to fig. 5, the basic detection unit 1 is further provided with an analysis reagent chamber, the storage detection chamber 15 of the analysis reagent chamber is connected through an analysis reagent control valve 23, the analysis reagent control valve 23 is connected with the driving assembly 40, and the driving assembly 40 drives the analysis reagent control valve 23 to switch between on and off through a third motor.

By arranging the analysis reagent chamber and the analysis reagent control valve 23, a large amount of analysis reagents can be accommodated, and the addition of the analysis reagents can be automatically controlled, so that the labor is saved, and the reaction analysis efficiency is improved.

Example six

On the basis of the first embodiment, the present invention further provides a linkage mechanism, wherein a valve core of the fluid push-out valve 22 is further provided with a stirring structure, and an output gear is arranged on an output shaft of the second motor 422.

The stirring mechanism is an incomplete gear, a part of tooth profile of the incomplete gear extends into the liquid storage detection chamber 15, and a part of tooth profile of the incomplete gear is meshed with the output gear of the second motor 422.

Wherein the tooth profile extending into the reservoir detection chamber 15 is immersed in the liquid to be detected in the reservoir detection chamber 15.

EXAMPLE seven

Based on the sixth embodiment and the second embodiment, the following steps are further included after the step S6:

s100, adding a detection reagent into the liquid storage detection chamber 15, wherein the detection reagent comprises one or more of sample lysate, enzyme, coenzyme, antibody, substrate, indicator, color developing agent, protective agent, confining liquid and reaction stopping liquid.

S200, controlling the driving assembly 40 to drive the second motor 422 to repeatedly swing within a preset angle range, so as to drive the incomplete gear to stir the liquid to be detected and the detection reagent in the liquid storage detection chamber 15, and thus accelerating the reaction.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. An automatic biochemical immunoassay device, comprising a basic detection unit, wherein the basic detection unit comprises a flow path module, a valve assembly, a push rod assembly and a driving assembly, wherein:

the flow path module includes the feed liquor cavity, inflow channel, ration cavity, outflow passageway and stock solution detection cavity, the inflow channel with the feed liquor cavity with the ration cavity links to each other, the outflow channel with ration cavity and stock solution detection cavity link to each other.

The valve assembly is connected with the inflow channel and the outflow channel, and the push rod assembly is connected with the quantitative chamber; the push rod the driving assembly is connected with the valve assembly and the push rod assembly and used for controlling the on-off of the valve assembly and the movement of the push rod assembly.

2. The automated biochemical immunoassay device of claim 1, wherein the inlet chamber is located higher than the quantifying chamber in the gravity direction.

3. The automated biochemical immunoassay device according to claim 1, wherein the push rod assembly comprises a rod body, a first limiting plate, a second limiting plate, a sealing member, a lock nut and a return spring, the first limiting plate and the second limiting plate are disposed at two ends of the rod body, the first limiting plate extends into the quantitative chamber, and the second limiting plate is located outside the quantitative chamber; and a position feedback sensor is arranged at one end of the rod body, which extends into the quantitative chamber, and is connected with the driving component.

4. An automated biochemical immunoassay device according to claim 3, wherein the position feedback sensor is a slide resistor, an electrode is disposed on the first limiting plate, a resistance means is disposed on a side wall of the quantifying chamber and is in electrical connection with the electrode during the resistance.

5. An automated biochemical immunoassay device according to claim 3, wherein said position feedback sensor is an image sensor, and a light source is further disposed in said quantitative chamber.

6. The automated biochemical immunoassay device of claim 1, wherein the automated biochemical immunoassay device comprises more than two sets of basic assay units, and a plurality of sets of the inflow channels are communicated with the same inlet chamber.

7. The automated biochemical immunoassay device according to claim 3, wherein the valve assembly comprises a fluid introduction valve and a fluid push-out valve, the fluid introduction valve being disposed on the inflow channel for controlling the opening and closing of the inflow channel; the fluid push-out valve is arranged on the outflow channel and used for controlling the on-off of the outflow channel; the valve cores of the fluid introducing valve and the fluid pushing valve are both rotary valve cores.

8. The automated biochemical immunoassay device according to claim 7, wherein a gear structure is further provided on the valve cores of the fluid inlet valve and the fluid outlet valve, and a thread structure matching with the gear is provided on the rod body, wherein the length of the thread structure can drive the rotation angle of the valve cores of the fluid inlet valve and the fluid outlet valve to be greater than 90 °; the matching relation between the gear structure arranged on the valve core of the fluid leading-in valve and the valve core of the fluid pushing-out valve and the thread structure arranged on the rod body is as follows: when the fluid introduction valve is in the on state, the fluid push-out valve is in the off state.

9. The detection method of the automatic biochemical immunoassay device is characterized by comprising the following steps:

and S1, controlling the first motor to drive the fluid introducing valve to be in a closed state, and controlling the second motor to drive the fluid ejecting valve to be in a conducting state.

10. The detection method of the automatic biochemical immunoassay device is characterized by comprising the following steps: