CN114966077B - Intelligent microorganism sampling detector for aquaculture waterline - Google Patents

Intelligent microorganism sampling detector for aquaculture waterline Download PDF

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Publication number
CN114966077B
CN114966077B CN202210913097.XA CN202210913097A CN114966077B CN 114966077 B CN114966077 B CN 114966077B CN 202210913097 A CN202210913097 A CN 202210913097A CN 114966077 B CN114966077 B CN 114966077B
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sampling
box
detection
electric
slide rail
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CN114966077A (en
Inventor
韩晓珺
张维秋
崔倡林
任巍峥
崔春祥
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Laizhou Animal Disease Prevention And Control Center
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Laizhou Animal Disease Prevention And Control Center
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention provides an intelligent microorganism sampling detector for a culture waterline, which relates to the technical field of sampling detection devices and comprises a sampling vehicle and a control system, wherein the sampling vehicle is provided with a three-dimensional adjusting frame, a conveying platform, a storage box and a detection box, a first material pushing mechanism is arranged in the storage box, a conveyor is arranged on the conveying platform, the conveying tail end of the conveyor is provided with a clamping mechanism, a detection mechanism, a bearing plate and a second material pushing mechanism are arranged in the detection box, the bearing plate is connected with a lifting mechanism, the control system comprises a processor, a communication module, electronic tags and a card reading module, and each electronic tag is arranged at a corresponding water fountain on the culture waterline. Meanwhile, the accuracy of the detection data is also ensured.

Description

Intelligent microorganism sampling detector for aquaculture waterline
Technical Field
The invention relates to the technical field of sampling detection devices, in particular to an intelligent microorganism sampling detector for a culture waterline.
Background
Farms typically use deep well, surface or tap water to supply water to the poultry through water supply towers, water delivery pipes, aquaculture lines, water fountains, and the like. However, the water supply tower, the water delivery pipeline, the breeding waterline and the water fountain are not cleaned and disinfected for a long time, so that moss grows and sludge accumulates in the water supply tower, the water delivery pipeline and the breeding waterline, a large amount of microorganisms breed in water, water quality is poor, and the morbidity and mortality of poultry can be increased by drinking water with the microorganisms exceeding standards for a long time, so that the water in the breeding waterline needs to be sampled and detected regularly.
At present, the most common sampling and detection mode is manual sampling and detection, sampling personnel need to sample and detect sampling points one by one, so that the labor intensity of the sampling personnel is very high, and the sampling and detection efficiency is very low.
Therefore, an intelligent microorganism sampling detector for a aquaculture water line is needed to solve the problems.
Disclosure of Invention
The invention provides an intelligent microorganism sampling detector for a culture waterline, which realizes batch automatic sampling and detection of the culture waterline, solves the problem of low manual sampling and detection efficiency, reduces the labor intensity of sampling personnel, improves the sampling and detection efficiency, and simultaneously ensures the accuracy of detection data.
The technical scheme of the invention is realized as follows:
the intelligent microorganism sampling detector for the culture waterline comprises a sampling vehicle, wherein a three-dimensional adjusting frame is arranged on the sampling vehicle, a horizontally arranged conveying platform is arranged on the three-dimensional adjusting frame, one end of the conveying platform is provided with a storage box for placing an unused sampling box, and the other end of the conveying platform is provided with a detection box for placing a used sampling box;
a sampling box outlet is formed in the side wall of the upper end of the storage box, and a first pushing mechanism is installed inside the storage box;
a conveyor is arranged on the conveying platform, the conveying front end of the conveyor is connected with the outlet of the sampling box, and a clamping mechanism is arranged at the conveying tail end of the conveyor;
the top of the detection box is provided with a sampling box inlet, the upper end of the inner side of the detection box is provided with a detection mechanism, one side of the detection mechanism is provided with a bearing plate, the bearing plate is vertically and slidably installed in the detection box, the bearing plate is connected with a lifting mechanism for driving the bearing plate to lift, and one side of the bearing plate is provided with a second material pushing mechanism;
the sampling box comprises a box body, a reagent groove and a sampling groove are arranged at the top of the box body, a reagent bottle is placed in the reagent groove, a push block is arranged on the three-dimensional adjusting frame and arranged above the clamping mechanism, a detection cavity is arranged below the reagent groove and the sampling groove, the reagent groove is communicated with the detection cavity through an inclined opening pipe, a sealing film covers an opening at the top of the sampling groove, the sampling groove is communicated with the detection cavity through a water inlet, and a condenser lens is arranged on one side wall of the detection cavity;
also includes a control system.
As a preferred technical scheme, the control system comprises a processor, a communication module and a plurality of electronic tags, each electronic tag is installed at a corresponding water fountain on the breeding waterline, a card reading module is installed on the conveying platform and adapted to the electronic tag, and the card reading module, the electronic tags and the communication module are electrically connected with the processor.
As a preferred technical scheme, a limiting ring is arranged in the reagent tank in a sliding mode, the limiting ring slides along the vertical direction, and a first compression spring is arranged between the bottom of the limiting ring and the bottom wall of the reagent tank.
As a preferred technical scheme, detection mechanism includes the casing, the casing is close to be equipped with mounting groove on the lateral wall of accepting the board, fixed mounting has the laser emitter of a vertical setting in the mounting groove, spherical lens, aspheric surface lens and dichroic mirror have set gradually under laser emitter, spherical lens and aspheric surface lens set up for the level, dichroic mirror sets up for the slope, dichroic mirror's inboard is provided with a filtering module, filtering module keeps away from one side of dichroic mirror is provided with photoelectric detection module, laser emitter and photoelectric detection module all with treater electric connection.
As a preferred technical scheme, first pushing equipment include along vertical slidable mounting in first push pedal in the storage box, the sample box place in on the first push pedal, the bottom of first push pedal with install second compression spring between the storage box diapire jointly, the inside upper end slidable mounting of storage box has the second push pedal, the position of second push pedal with the position of sample box export is corresponding, the second push pedal is kept away from the one end of sample box export is connected with first electric putter, first electric putter with treater electric connection.
As a preferred technical scheme, fixture includes the clamping jaw of a pair of relative setting, conveying platform is close to the one end slidable mounting of detection case has a pair of mount pad, the mount pad is followed the direction of delivery of sampling box slides, the mount pad is connected with second electric putter, each the equal slidable mounting of clamping jaw is in corresponding on the mount pad, each the clamping jaw all is connected with a third electric putter, second electric putter and third electric putter all with treater electric connection.
As a preferred technical scheme, the second pushing mechanism comprises a vertically arranged third pushing plate, the third pushing plate is slidably mounted in the detection box along the horizontal direction, the third pushing plate is arranged on one side, close to the detection mechanism, of the receiving plate, the third pushing plate is in transmission connection with a fourth electric push rod, and the fourth electric push rod is electrically connected with the processor.
As a preferable technical proposal, the three-dimensional adjusting bracket comprises a first electric sliding rail which is horizontally arranged, the first electric slide rail is fixedly arranged at the top of the sampling vehicle, a first sliding seat is arranged on the first electric slide rail, the first sliding seat is provided with a second electric sliding rail which is vertical to the first electric sliding rail, a second sliding seat is arranged on the second electric sliding rail, a vertical bracket is fixed on the second sliding seat, the push block is arranged on the vertical bracket, a third electric slide rail is arranged on the vertical bracket, the third electric slide rail is vertical to the first electric slide rail and the second electric slide rail, the conveying platform is arranged on the third electric slide rail, the first electric slide rail, the second electric slide rail and the third electric slide rail are all electrically connected with the processor.
As a preferred technical scheme, elevating system include along the horizontal direction install in the transmission shaft at detection box top, install a servo motor on the detection box, servo motor's motor shaft with the transmission shaft transmission is connected, a drive gear is all installed at the both ends of transmission shaft, each drive gear's one side all is provided with a driven gear, each driven gear all through the spool rotation install in on the detection box, each driven gear all with drive gear meshes mutually, the both ends of accepting the board respectively fixedly connected with haulage rope, each haulage rope all twine in on the spool, servo motor with treater electric connection.
By adopting the technical scheme, the invention has the beneficial effects that:
according to the invention, a sampling box is pushed onto the conveying platform by using a first pushing mechanism in the storage box, the sampling box is conveyed by using a conveyor on the conveying platform, is clamped by using a clamping mechanism on the conveying platform, is placed between a water fountain and a suspension cup through the sampling vehicle and the three-dimensional adjusting frame, and is finally sampled by upwards pressing the water fountain by using the sampling box, so that the automatic sampling of the culture waterline is realized; after the sampling work is completed, the sampling box is placed into the detection box, and the water sample in the sampling box is automatically detected, so that the automatic sampling and detection of the batch of the aquaculture waterline are realized, the manual sampling and detection work of sampling personnel is not needed, the problem of slow manual sampling and detection efficiency is solved, the labor intensity of the sampling personnel is greatly reduced, and the efficiency of the sampling and detection work is improved.
According to the invention, the receiving plate is used for receiving the sampling boxes entering the detection box, the lifting mechanism is used for driving the receiving plate to move up and down, the sampling boxes can be aligned with the detection mechanism one by one in the process that the receiving plate drives the sampling boxes to move downwards, the detection mechanism is used for detecting the content of microorganisms in the water samples, automatic detection of the water samples in the sampling boxes is realized, the second pushing mechanism is used for pushing the sampling boxes on the receiving plate to the positions on the rear side of the receiving plate, so that the receiving plate can be lifted to the initial positions to continue to receive the sampling boxes, subsequent sampling boxes can enter the detection box for detection, further batch detection of the sampling boxes is realized, and the sampling and detection work efficiency is improved.
Because the sampling box comprises a box body, a reagent groove, a sampling groove and a detection cavity are arranged in the box body, a reagent bottle is placed in the reagent groove, a push block is arranged on the three-dimensional adjusting frame, in the invention, when the sampling box is used for sampling, the bottom of the water fountain punctures the sealing film at the top of the sampling groove, the bottom wall of the sampling groove is used for pushing the water fountain, water in the waterline flows into the sampling groove and the detection cavity through the water fountain, meanwhile, the pushing block has a limiting effect on the reagent bottle, so that the reagent bottle moves downwards in the reagent tank, the sealing film paper at the position of the reagent bottle mouth is punctured by the beveled pipe at the bottom of the reagent tank, and a detection reagent in the reagent bottle flows into the detection cavity through the beveled pipe, so that the detection reagent and a water sample are automatically mixed, the detection mechanism can conveniently and automatically detect the water sample in the sampling box in the subsequent process, and the detection efficiency is improved; and before sampling, sampling groove and reagent bottle all are in encapsulated situation, have avoided influencing the condition emergence of testing result because of sampling groove, detection chamber and detect reagent are contaminated to the accuracy of testing data has been guaranteed.
According to the invention, the electronic tags and the card reading modules are utilized to realize the automatic positioning work of the sampling vehicle, each electronic tag corresponds to a position number, and after the card reading module reads the position number to be sampled, the sampling vehicle automatically stops and performs sampling work at the corresponding water fountain, so that the automatic sampling of the aquaculture waterline is realized.
Because the limiting ring and the first compression spring are arranged in the reagent groove, in the invention, the limiting ring and the first compression spring are arranged, on one hand, the bottle mouth of the reagent bottle is separated from the inclined mouth pipe, the sealing performance of the reagent bottle is ensured, the condition that a detection result is influenced because a detection reagent is polluted is avoided, on the other hand, the reagent bottle has a certain movement amount, when sampling is carried out, the reagent bottle can move downwards under the limiting effect of the push block, the sealing membrane paper at the bottle mouth of the reagent bottle is punctured by using the inclined mouth pipe, the detection reagent enters the detection cavity to be mixed with a water sample, and the smooth proceeding of detection work and the accuracy of detection data are ensured.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without inventive exercise.
FIG. 1 is a sample schematic of the present invention;
FIG. 2 is a schematic structural view of the present invention;
FIG. 3 is a schematic sectional view taken along line A-A of FIG. 2;
FIG. 4 is a schematic cross-sectional view taken along line B-B of FIG. 2;
FIG. 5 is a schematic view of a cartridge and detection mechanism according to the present invention;
fig. 6 is a schematic structural diagram of a control system according to the present invention.
Wherein: 1. sampling vehicles; 2. a three-dimensional adjusting frame; 3. a conveying platform; 4. a sampling box; 5. a storage box; 6. a detection box; 7. an outlet of the sample cartridge; 8. a conveyor; 9. an inlet of the sampling box; 10. a bearing plate; 11. a culture water line; 12. a water fountain; 13. a box body; 14. a reagent tank; 15. a sampling slot; 16. a reagent bottle; 17. a push block; 18. a detection chamber; 19. a beveled pipe; 20. a sealing film; 21. a water inlet; 22. a condenser lens; 23. a processor; 24. a communication module; 25. an electronic tag; 26. a card reading module; 27. a control terminal; 28. a limiting ring; 29. a first compression spring; 30. a housing; 31. a dichroic mirror; 32. a light filtering module; 33. a photoelectric detection module; 34. a first push plate; 35. a second compression spring; 36. a second push plate; 37. a first electric push rod; 38. a clamping jaw; 39. a mounting seat; 40. a second electric push rod; 41. a third electric push rod; 42. a third push plate; 43. a fourth electric push rod; 44. a first electric slide rail; 45. a first sliding seat; 46. a second electric slide rail; 47. a second sliding seat; 48. a vertical support; 49. a third electric slide rail; 50. a drive shaft; 51. a servo motor; 52. a drive gear; 53. a driven gear; 54. a spool; 55. a hauling rope; 56. installing a groove; 57. a laser transmitter; 58. a spherical lens; 59. an aspherical lens; 60. and (4) hanging the cup.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-6, the intelligent microorganism sampling detector for the aquaculture waterline comprises a sampling vehicle 1, wherein a three-dimensional adjusting frame 2 is mounted on the sampling vehicle 1, a conveying platform 3 horizontally arranged is mounted on the three-dimensional adjusting frame 2, one end of the conveying platform 3 is provided with a storage box 5 for storing an unused sampling box 4, the other end of the conveying platform 3 is provided with a detection box 6 for storing a used sampling box 4, and in this embodiment, the sampling vehicle 1 can adopt a commercially available crawler flat car.
A sampling box outlet 7 is formed in the side wall of the upper end of the storage box 5, a first pushing mechanism is mounted inside the storage box 5, and the sampling box 4 is pushed out of the storage box 5 through the sampling box outlet 7 by the first pushing mechanism.
Install a conveyer 8 on conveying platform 3, conveyer 8 is used for carrying sampling box 4, and conveyer 8's transport front end links up with sampling box export 7 mutually, and conveyer 8's transport end is provided with a fixture, and fixture is used for carrying out the centre gripping to sampling box 4, and in this embodiment, conveyer 8 can adopt the miniature belt conveyor who founds the ssj7811 model that magnesium mechanical equipment limited made.
The top of the detection box 6 is provided with a sampling box inlet 9, the sampling box 4 enters the detection box 6 through the sampling box inlet 9, a detection mechanism is installed at the upper end of the inner side of the detection box 6, one side of the detection mechanism is provided with a bearing plate 10, the bearing plate 10 is installed in the detection box 6 along the vertical sliding direction, the bearing plate 10 is connected with a lifting mechanism used for driving the bearing plate 10 to lift, and a second material pushing mechanism is installed on one side of the bearing plate 10.
In the invention, the sampling box 4 is used for automatic sampling at the corresponding water fountain 12 on the culture waterline 11, and the sampling box 4 is conveyed into the detection box 6 for automatic detection and storage after sampling is finished, thereby realizing batch automatic sampling and detection of the culture waterline 11.
The sampling box 4 comprises a box body 13, a reagent tank 14 and a sampling tank 15 are arranged at the top of the box body 13, a reagent bottle 16 is placed in the reagent tank 14, the reagent bottle 16 is placed in the reagent tank 14 in an inverted mode, a push block 17 is installed on the three-dimensional adjusting frame 2, the push block 17 is arranged above the clamping mechanism, and the push block 17 is used for pressing the corresponding reagent bottle 16; a detection cavity 18 is arranged below the reagent tank 14 and the sampling tank 15, the reagent tank 14 is communicated with the detection cavity 18 through a bevel pipe 19, a sealing film 20 covers the top opening of the sampling tank 15, the sampling tank 15 is communicated with the detection cavity 18 through a water inlet 21, a condenser lens 22 is installed on one side wall of the detection cavity 18, and in the embodiment, the condenser lens 22 can be a spherical lens supported by plastics.
In the invention, before the sampling box 4 is used, the sampling groove 15 and the reagent bottle 16 are in a sealed state, so that the condition that the detection result is influenced because the sampling box 4 and the detection reagent are polluted is avoided, the accuracy of detection data is ensured, meanwhile, the automatic mixing of a water sample and the detection reagent is realized, and the efficiency of sampling and detection work is improved.
The invention also comprises a control system, the control system comprises a processor 23, a communication module 24 and a plurality of electronic tags 25, each electronic tag 25 is arranged at the corresponding water fountain 12 on the aquaculture water line 11, a card reading module 26 is arranged on the conveying platform 3, the card reading module 26 is matched with the electronic tag 25, the card reading module 26, the electronic tag 25 and the communication module 24 are electrically connected with the processor 23, in the embodiment, the processor 23 can adopt an ARM9 series S3C2440 type main control chip, the communication module 24 can adopt an HF-LPT220-S88 type WIFI communication module, the processor 23 is in communication connection with a control terminal 27 in the hand of a sampling person through the communication module 24, the electronic tag 25 can adopt a 915 type C116301 ultra-high frequency ISO RFID tag card produced by high-tech electronics, the card reading module 26 can adopt a C216022 type Gen 960MHz four-port Gen-plus 960MHz RFID reader-writer produced by high-tech electronics, in the invention, each water fountain 12 of the aquaculture waterline 11 is provided with an electronic tag 25, each electronic tag 25 corresponds to a position number, the electronic tag 25 and the card reading module 26 are used for automatically positioning the sampling position, and after the positioning is finished, the sampling box 4 is used for sampling at the water fountain 12 corresponding to the sampling position, thereby realizing the automatic sampling of the aquaculture waterline 11.
As shown in fig. 1-5, a limiting ring 28 is slidably mounted in the reagent tank 14, the limiting ring 28 slides in a vertical direction, and a first compression spring 29 is mounted between the bottom of the limiting ring 28 and the bottom wall of the reagent tank 14.
Wherein, the detection mechanism includes a housing 30, a mounting groove 56 is provided on a side wall of the housing 30 close to the receiving plate 10, a vertically arranged laser emitter 57 is fixedly mounted in the mounting groove 56, a spherical lens 58, an aspheric lens 59 and a dichroic mirror 31 are sequentially provided under the laser emitter 57, the spherical lens 58 and the aspheric lens 59 are horizontally arranged, the dichroic mirror 31 is obliquely arranged, a filter module 32 is provided on an inner side of the dichroic mirror 31, a photoelectric detection module 33 is provided on a side of the filter module 32 away from the dichroic mirror 31, the laser emitter 57 and the photoelectric detection module 33 are both electrically connected with the processor 23, in this embodiment, the laser emitter 57 may be a laser diode, a laser color emitted by the laser diode is selected according to a fluorescent substance used in the detection reagent, the dichroic mirror 31 is a glass lens with a surface coating, dichroic mirror 31 and filtering module 32 can be selected according to the color of the fluorescence generated after the excitation of the fluorescent substance, and photo-detector module 33 is a photo-detector composed of a plurality of photodiodes.
As shown in fig. 1-4, the first pushing mechanism includes a first pushing plate 34 installed in the storage box 5 in a sliding manner along the vertical direction, the sample cartridge 4 is placed on the first pushing plate 34, a second compression spring 35 is installed between the bottom of the first pushing plate 34 and the bottom wall of the storage box 5, a second pushing plate 36 is installed in the storage box 5 in a sliding manner at the upper end, the position of the second pushing plate 36 corresponds to the position of the outlet 7 of the sample cartridge, a first electric push rod 37 is connected to one end of the second pushing plate 36 away from the outlet 7 of the sample cartridge, the first electric push rod 37 is electrically connected to the processor 23, in this embodiment, the first electric push rod 37 may be a UT series electric push rod produced by wuxielitt linear motion mechanical limited, in the present invention, the second pushing plate 36 is used for pushing out the storage box 5 of the sample cartridge 4 located at the uppermost layer, when the second pushing plate 36 is retracted to one side, the first push plate 34 pushes the sample cartridge 4 to move upward under the elastic force of the second compression spring 35, and then the second push plate 36 pushes the sample cartridge 4 located at the uppermost layer out of the storage box 5.
Furthermore, the clamping mechanism comprises a pair of clamping jaws 38 which are oppositely arranged, one end of the conveying platform 3 close to the detection box 6 is provided with a pair of mounting seats 39 in a sliding manner, the mounting seats 39 slide along the conveying direction of the sampling box 4, the mounting seats 39 are connected with a second electric push rod 40, the second electric push rod 40 is arranged on the outer side of the conveying platform 3, the second electric push rod 40 extends along the length direction of the conveying platform 3, each clamping jaw 38 is arranged on the corresponding mounting seat 39 in a sliding manner, each clamping jaw 38 slides along the width direction of the conveying platform 3, each clamping jaw 38 is connected with a third electric push rod 41, the second electric push rod 40 and the third electric push rod 41 are both electrically connected with the processor 23, in the present embodiment, the UT-series electric push rods manufactured by wuxist elter linear motion machine ltd may be used as both the second electric push rod 40 and the third electric push rod 41.
The second pushing mechanism comprises a vertically arranged third pushing plate 42, the third pushing plate 42 is slidably mounted in the detection box 6 along the horizontal direction, the third pushing plate 42 is arranged on one side of the bearing plate 10 close to the detection mechanism, the third pushing plate 42 is driven by a fourth electric push rod 43 to slide, the fourth electric push rod 43 is electrically connected with the processor 23, and in this embodiment, the fourth electric push rod 43 can adopt a UT-series electric push rod produced by the stannless einter linear motion mechanical limited company.
In addition, the three-dimensional adjusting frame 2 includes a first electric slide rail 44 horizontally arranged, the first electric slide rail 44 is fixedly installed at the top of the sampling vehicle 1, a first sliding seat 45 is installed on the first electric slide rail 44, the first sliding seat 45 slides along the extending direction of the first electric slide rail 44, a second electric slide rail 46 is installed on the first sliding seat 45, the second electric slide rail 46 is perpendicular to the first electric slide rail 44, a second sliding seat 47 is installed on the second electric slide rail 46, the second sliding seat 47 slides along the extending direction of the second electric slide rail 46, a vertical support 48 is fixed on the second sliding seat 47, the push block 17 is installed on the vertical support 48, a third electric slide rail 49 is installed on the vertical support 48, the third electric slide rail 49 is perpendicular to the first electric slide rail 44 and the second electric slide rail 46, the conveying platform 3 is installed on the third electric slide rail 49, the first electric slide rail 44, the second electric slide rail 46, and the third electric slide rail 46 are installed on the sampling vehicle 1, and the third electric slide rail 49 is installed on the vertical support 48, the first electric slide rail 44, Second electronic slide rail 46 and third electronic slide rail 49 all with treater 23 electric connection, in this embodiment, first electronic slide rail 44, second electronic slide rail 46 and third electronic slide rail 49 all can adopt the roller type linear slideway of RGH25CA model that HIWIN made.
Elevating system includes and installs in the transmission shaft 50 at detection case 6 top along the horizontal direction, install a servo motor 51 on the detection case 6, servo motor 51's motor shaft and transmission shaft 50 transmission are connected, a drive gear 52 is all installed at the both ends of transmission shaft 50, one side of each drive gear 52 all is provided with a driven gear 53, each driven gear 53 all through the spool 54 rotation mounting on detection case 6, each driven gear 53 all meshes with drive gear 52 mutually, the both ends of accepting board 10 are a fixedly connected with haulage rope 55 respectively, each haulage rope 55 all twines on spool 54, servo motor 51 and treater 23 electric connection.
The method for sampling the culture water line 11 by using the invention comprises the following steps:
firstly, a sampling person sets a position number to be sampled through the control terminal 27, the control terminal 27 transmits the set position number to the processor 23, the sampling vehicle 1 is placed at a proper position in a poultry house, the sampling vehicle 1 is controlled to start, and the sampling vehicle 1 moves forwards;
secondly, in the moving process of the sampling vehicle 1, reading the electronic tag 25 at the position of the water dispenser 12 by the card reading module 26, and when the read position number of the electronic tag 25 belongs to the preset position number, controlling the sampling vehicle 1 to stop by the processor 23 to enable the sampling vehicle 1 to stop beside the corresponding water dispenser 12, wherein the conveying platform 3 is aligned with the corresponding water dispenser 12;
thirdly, the processor 23 controls the first electric push rod 37 to start, the first electric push rod 37 drives the second push plate 36 to move towards the direction far away from the outlet 7 of the sampling box, so that the second push plate 36 is no longer pressed on the top of the sampling box 4 on the uppermost layer, under the action of the elastic force of the second compression spring 35, the first push plate 34 moves upwards to drive the sampling box 4 to move upwards, and the top of the sampling box 4 on the uppermost layer is abutted against the top wall of the storage box 5, then the first electric push rod 37 is started again, the first electric push rod 37 drives the second push plate 36 to move towards the direction close to the outlet 7 of the sampling box, and in the moving process of the second push plate 36, the sampling box 4 on the uppermost layer is pushed to move, so that the sampling box 4 closest to the outlet 7 of the sampling box leaves the storage box 5 through the outlet 7 of the sampling box, and the sampling box 4 is pushed onto the conveyor 8;
fourthly, the processor 23 controls the first electric push rod 37 to stop and the conveyor 8 to start, the conveyor 8 drives the sampling box 4 to move towards the direction close to the water fountain 12, when the sampling box 4 moves between the two clamping jaws 38, the processor 23 controls the conveyor 8 to stop and the third electric push rod 41 to start, and the third electric push rod 41 drives the two clamping jaws 38 to clamp the sampling box 4;
fifthly, after the sampling box 4 is clamped, the processor 23 controls the first electric slide rail 44 to start, the first electric slide rail 44 drives the conveying platform 3 to horizontally slide, and the sampling box 4 finally extends into a position between the water fountain 12 and the hanging cup 60, and at the moment, the sampling groove 15 of the sampling box 4 is aligned with the water fountain 12;
sixthly, the processor 23 controls the first electric slide rail 44 to stop, the third electric slide rail 49 is started, the third electric slide rail 49 drives the conveying platform 3 and the sampling box 4 to move upwards, the bottom of the water fountain 12 is inserted into the sampling groove 15 after puncturing the sealing film 20, the bottom wall of the sampling groove 15 is used for pushing the bottom of the water fountain 12, water in the aquaculture water line 11 flows into the sampling groove 15 through the water fountain 12 and enters the detection cavity 18 through the water inlet 21, meanwhile, the pushing block 17 on the vertical support 48 limits the reagent bottle 16 and pushes the reagent bottle 16 to slide downwards in the reagent groove 14, the top of the oblique mouth pipe 19 is inserted into the reagent bottle 16 after puncturing the sealing film paper at the bottle mouth of the reagent bottle 16, and the detection reagent in the reagent bottle 16 flows into the detection cavity 18 through the oblique mouth pipe 19 and is mixed with the water sample in the detection cavity 18;
seventhly, after the sampling work is finished, the processor 23 controls the first electric slide rail 44, the third electric slide rail 49 and the second electric push rod 40 to start, the first electric slide rail 44 and the third electric slide rail 49 drive the conveying platform 3 to return, the second electric push rod 40 pushes the mounting seat 39, the clamping jaw 38 and the sampling box 4 to move, so that the sampling box 4 is aligned with the sampling box outlet 7 at the top of the detection box 6, then the processor 23 controls the second electric push rod 40 to stop, the third electric push rod 41 to start, the third electric push rod 41 drives the clamping jaw 38 to move outwards to loosen the sampling box 4, the sampling box 4 falls under the action of gravity and falls onto the bearing plate 10 in the detection box 6 through the sampling box inlet 9, meanwhile, the processor 23 controls the third electric push rod 41 to stop, the second electric push rod 40 to open, and the second electric push rod 40 drives the clamping jaw 38 to return;
eighthly, after the sampling box 4 falls on the receiving plate 10, the processor 23 controls the servo motor 51 to start, the servo motor 51 drives the winding shaft 54 to rotate through the power transmission of the driving gear 52 and the driven gear 53, the traction rope 55 on the winding shaft 54 is released, the receiving plate 10 moves downwards under the action of gravity, and after the receiving plate 10 falls for a certain distance, the processor 23 controls the servo motor 51 to stop, so that the automatic sampling work at the water fountain 12 is completed;
ninthly, after the sampling work of the waterer 12 is finished, the processor 23 controls the sampling vehicle 1 to start, the sampling vehicle 1 continues to advance, and the operations are continuously repeated to finish the sampling work at other sampling positions;
tenth, in the process that the carrier plate 10 moves downward, the collecting mirror 22 on the sampling box 4 is aligned with the dichroic mirror 31, the processor 23 controls the laser emitter 57 and the photoelectric detection module 33 to start, the laser emitted by the laser emitter 57 is collimated by the spherical lens 58 and the aspherical lens 59 and then irradiated onto the dichroic mirror 31, the collimated laser is reflected by the dichroic mirror 31 to horizontally irradiate the collecting mirror 22, the collecting mirror 22 collects the laser, the collected laser is projected into the detection cavity 18 to excite the water sample in the detection cavity 18, the water sample emits fluorescence after being excited, a part of the excited fluorescence returns along the original path of the laser and reaches the filter module 32 after sequentially passing through the collecting mirror 22 and the dichroic mirror 31, stray light contained in the fluorescence is filtered by the filter module 32, and then the fluorescence irradiates the photoelectric detection module 33, the photoelectric detection module 33 is used for detecting the intensity of the fluorescence, the detected light intensity data is transmitted to the processor 23, the processor 23 is used for calculating, and the calculated data is transmitted to the control terminal 27 of the sampling personnel through the communication module 24, so that the sampling personnel can analyze the content of the microorganisms in the water sample by using the obtained data;
step eleven, after the bearing plate 10 falls to the lowest position, the processor 23 controls the fourth electric push rod 43 to start, the fourth electric push rod 43 drives the third push plate 42 to move towards the direction close to the sampling box 4 and pushes the sampling box 4 to move backwards to push the sampling box 4 to a proper position, after the material pushing action is completed, the fourth electric push rod 43 drives the third push plate 42 to move backwards and return to the position, then the processor 23 controls the fourth electric push rod 43 to stop, the servo motor 51 to start reversely, and the servo motor 51 drives the winding shaft 54 to rotate reversely to wind the traction rope 55 on the winding shaft 54, so that the bearing plate 10 is pulled to rise, and a subsequent sampling box 4 is borne.
In summary, the intelligent microorganism sampling detector for the aquaculture waterline provided by the invention realizes batch automatic sampling and detection of the aquaculture waterline 11, solves the problem of slow manual sampling and detection efficiency, reduces the labor intensity of sampling personnel, improves the sampling and detection efficiency, and simultaneously ensures the accuracy of detection data.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (8)

1. The intelligent microorganism sampling detector for the aquaculture waterline comprises a sampling vehicle (1) and is characterized in that a three-dimensional adjusting frame (2) is mounted on the sampling vehicle (1), a conveying platform (3) which is horizontally arranged is mounted on the three-dimensional adjusting frame (2), a storage box (5) for storing an unused sampling box (4) is arranged at one end of the conveying platform (3), and a detection box (6) for storing the used sampling box (4) is arranged at the other end of the conveying platform (3);
a sampling box outlet (7) is formed in the side wall of the upper end of the storage box (5), and a first pushing mechanism is mounted inside the storage box (5);
a conveyor (8) is mounted on the conveying platform (3), the conveying front end of the conveyor (8) is connected with the sampling box outlet (7), and a clamping mechanism is arranged at the conveying tail end of the conveyor (8);
a sampling box inlet (9) is formed in the top of the detection box (6), a detection mechanism is installed at the upper end of the inner side of the detection box (6), a bearing plate (10) is arranged on one side of the detection mechanism, the bearing plate (10) is vertically and slidably installed in the detection box (6), the bearing plate (10) is connected with a lifting mechanism used for driving the bearing plate (10) to lift, and a second material pushing mechanism is installed on one side of the bearing plate (10);
the sampling box (4) comprises a box body (13), a reagent groove (14) and a sampling groove (15) are arranged at the top of the box body (13), a reagent bottle (16) is placed in the reagent groove (14), a push block (17) is installed on the three-dimensional adjusting frame (2), the push block (17) is arranged above the clamping mechanism, a detection cavity (18) is arranged below the reagent groove (14) and the sampling groove (15), the reagent groove (14) is communicated with the detection cavity (18) through an inclined opening pipe (19), a sealing film (20) covers an opening at the top of the sampling groove (15), the sampling groove (15) is communicated with the detection cavity (18) through a water inlet (21), and a condenser lens (22) is installed on one side wall of the detection cavity (18);
the water fountain device is characterized by further comprising a control system, wherein the control system comprises a processor (23), a communication module (24) and a plurality of electronic tags (25), each electronic tag (25) is installed at a corresponding water fountain (12) on the cultivation waterline (11), a card reading module (26) is installed on the conveying platform (3), the card reading module (26) is matched with the electronic tags (25), and the card reading module (26), the electronic tags (25) and the communication module (24) are electrically connected with the processor (23).
2. The intelligent sampling detector for the microorganisms in the aquaculture water line according to claim 1, characterized in that a limiting ring (28) is slidably mounted in the reagent tank (14), the limiting ring (28) slides in the vertical direction, and a first compression spring (29) is commonly mounted between the bottom of the limiting ring (28) and the bottom wall of the reagent tank (14).
3. The intelligent microorganism sampling detector for aquaculture waterlines of claim 2, the detection mechanism comprises a shell (30), a mounting groove (56) is arranged on one side wall of the shell (30) close to the bearing plate (10), a vertically arranged laser emitter (57) is fixedly arranged in the mounting groove (56), a spherical lens (58), an aspherical lens (59) and a dichroic mirror (31) are sequentially arranged right below the laser emitter (57), the spherical lens (58) and the aspherical lens (59) are horizontally arranged, the dichroic mirror (31) is obliquely arranged, a light filtering module (32) is arranged on the inner side of the dichroic mirror (31), a photoelectric detection module (33) is arranged on one side of the light filtering module (32) far away from the dichroic mirror (31), the laser emitter (57) and the photoelectric detection module (33) are electrically connected with the processor (23).
4. The intelligent sampling detector for microorganisms in aquaculture water lines according to claim 3, wherein the first pushing mechanism comprises a first push plate (34) which is vertically slidably mounted in the storage box (5), the sample box (4) is placed on the first push plate (34), a second compression spring (35) is commonly mounted between the bottom of the first push plate (34) and the bottom wall of the storage box (5), a second push plate (36) is slidably mounted at the upper end of the interior of the storage box (5), the position of the second push plate (36) corresponds to the position of the outlet (7) of the sample box, one end, away from the outlet (7) of the sample box, of the second push plate (36) is connected with a first electric push rod (37), and the first electric push rod (37) is electrically connected with the processor (23).
5. The intelligent aquaculture water line microorganism sampling detector as claimed in claim 4, wherein the clamping mechanism comprises a pair of oppositely arranged clamping jaws (38), a pair of mounting seats (39) is slidably mounted at one end, close to the detection box (6), of the conveying platform (3), the mounting seats (39) slide along the conveying direction of the sampling boxes (4), second electric push rods (40) are connected to the mounting seats (39), each clamping jaw (38) is slidably mounted on the corresponding mounting seat (39), a third electric push rod (41) is connected to each clamping jaw (38), and the second electric push rod (40) and the third electric push rod (41) are electrically connected with the processor (23).
6. The intelligent sampling detector for the aquaculture water line microorganisms, as recited in claim 5, wherein the second pushing mechanism comprises a vertically arranged third pushing plate (42), the third pushing plate (42) is installed in the detection box (6) in a sliding manner along a horizontal direction, the third pushing plate (42) is arranged on one side of the receiving plate (10) close to the detection mechanism, the third pushing plate (42) is in transmission connection with a fourth electric push rod (43), and the fourth electric push rod (43) is electrically connected with the processor (23).
7. The intelligent sampling detector for microorganisms in aquaculture water lines according to claim 6, wherein the three-dimensional adjusting frame (2) comprises a first electric slide rail (44) horizontally arranged, the first electric slide rail (44) is fixedly installed at the top of the sampling vehicle (1), a first sliding seat (45) is installed on the first electric slide rail (44), a second electric slide rail (46) is installed on the first sliding seat (45), the second electric slide rail (46) is perpendicular to the first electric slide rail (44), a second sliding seat (47) is installed on the second electric slide rail (46), a vertical support (48) is fixed on the second sliding seat (47), the pushing block (17) is installed on the vertical support (48), a third electric slide rail (49) is installed on the vertical support (48), and the third electric slide rail (49) is perpendicular to the first electric slide rail (44) and the second electric slide rail (46) The conveying platform (3) is installed on the third electric sliding rail (49), and the first electric sliding rail (44), the second electric sliding rail (46) and the third electric sliding rail (49) are electrically connected with the processor (23).
8. The intelligent sampling detector for microorganisms in aquaculture water lines according to claim 7, wherein the lifting mechanism comprises a transmission shaft (50) installed at the top of the detection box (6) along the horizontal direction, a servo motor (51) is installed on the detection box (6), a motor shaft of the servo motor (51) is in transmission connection with the transmission shaft (50), a driving gear (52) is installed at both ends of the transmission shaft (50), a driven gear (53) is arranged at one side of each driving gear (52), each driven gear (53) is rotatably installed on the detection box (6) through a winding shaft (54), each driven gear (53) is meshed with the driving gear (52), a pulling rope (55) is fixedly connected to both ends of the bearing plate (10), and each pulling rope (55) is wound on the winding shaft (54), the servo motor (51) is electrically connected with the processor (23).
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