CN114966017A - New coronavirus antigen detection kit based on fluorescence immunochromatography - Google Patents
New coronavirus antigen detection kit based on fluorescence immunochromatography Download PDFInfo
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- CN114966017A CN114966017A CN202210582441.1A CN202210582441A CN114966017A CN 114966017 A CN114966017 A CN 114966017A CN 202210582441 A CN202210582441 A CN 202210582441A CN 114966017 A CN114966017 A CN 114966017A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a new coronavirus antigen detection kit, which relates to the technical field of medical supplies and comprises a kit main body, wherein a test board is arranged inside the kit main body, a fixed block is arranged on one side of the top of the kit main body, the inside of the fixed block is connected with the test board through a clamping component, an installation block is arranged on the top of the fixed block, one side of the installation block is connected with a support plate through an installation component, and the test board comprises a PVC (polyvinyl chloride) base pad, an NC (numerical control) membrane, a test wire, a control wire, a sample pad, a colloidal gold pad and a water absorption pad. The invention relates to a new coronavirus antigen detection kit, which avoids the condition that a test board deviates during detection, reduces other risks of detection, improves the quality and efficiency of antigen detection, can quickly detect antigens, and ensures the efficiency and quality of new coronavirus antigen detection.
Description
Technical Field
The invention relates to the technical field of medical supplies, in particular to a novel coronavirus antigen detection kit based on a fluorescence immunochromatography method.
Background
The spread of the new coronavirus is mainly the respiratory droplets in the close range, and other routes, such as contact, fecal inlet (digestive tract) and the like, have great possibility.
Reagents for detecting IgM and IgG antibodies of the novel coronavirus are also known at present, and IgM screening for suspected cases of the novel coronavirus is also called for by some doctors. After the novel coronavirus infects organism, the genetic material of the virus, RNA, is the first marker to be detected. Along with the development of the disease course after infection, the human immune system can generate specific antibodies aiming at structural proteins of viruses, wherein IgM (immunoglobulin m) is an early antibody generated after the body is infected and indicates the infected state at the present stage; while IgG suggests a past history of infection. However, both IgM and IgG have a window period from viral infection to antibody production, and therefore detection tends to lag behind nucleic acid detection, and it is generally considered that specific antibody detection is also less sensitive than nucleic acid detection.
In the existing market, when antigen detection is carried out, the test paper is singly placed, and needs to be manually taken and placed, but under the condition that the test paper is not fixed, the test paper is easy to deviate, so that samples are scattered, the dropping sample detection effect is reduced, the antigen detection is influenced by other factors, and the detection is inconvenient and rapid, so that the new coronavirus antigen detection kit is necessary to be provided.
Disclosure of Invention
The invention mainly aims to provide a novel coronaviruses antigen detection kit, which can effectively solve the problems that in the existing antigen detection kit in the background technology, when antigen detection is carried out, test paper is singly placed and needs to be manually taken and placed, but under the condition that the test paper is not fixed, the test paper is easy to deviate, samples are scattered, the drop sample detection effect is reduced, the antigen detection is influenced by other factors, and the rapid detection is inconvenient.
In order to achieve the purpose, the invention adopts the technical scheme that: a new coronavirus antigen detection kit comprises a kit main body, wherein a test board is arranged inside the kit main body, a fixed block is arranged on one side of the top of the kit main body, the inside of the fixed block is connected with the test board through a clamping component, an installation block is arranged on the top of the fixed block, and one side of the installation block is connected with a support plate through an installation component;
the test board comprises a PVC base pad, an NC membrane, a test wire, a control wire, a sample pad, a colloidal gold pad and a water absorption pad, wherein the PVC base pad is arranged at the bottom of the test board, the NC membrane is fixedly connected to the center of the top of the PVC base pad, the test wire and the control wire are sequentially arranged on the top of the NC membrane from left to right, the sample pad is arranged on one side of the top of the PVC base pad, the colloidal gold pad is connected to the bottom of one side of the sample pad, and the water absorption pad is arranged on the other side of the top of the PVC base pad;
the clamping assembly comprises a sleeve plate, a limiting plate, a fixing rod, a first spring, a second spring and a clamping plate, wherein an insertion groove is formed in one side of the fixing block, cavities are formed in two opposite inner walls in the insertion groove, the sleeve plate is movably arranged in the cavities, one end of the sleeve plate is fixedly connected with one side of the limiting plate, the fixing rod is fixedly connected in the cavities, the first spring is sleeved in the cavity on the outer peripheral surface of the fixing rod, the second spring is fixedly connected in the cavity of the sleeve plate at one end of the fixing rod, and one end of the sleeve plate is fixedly connected with one side of the clamping plate;
the mounting assembly comprises a hexagonal rod, a connecting block, a fixing pin and external threads, wherein a groove is formed in one side of the top of the mounting block, the hexagonal rod is fixedly connected with the inside bottom plate of the groove in the vertical direction, one end of the supporting plate is fixedly connected with one side of the connecting block, a hexagonal hole is formed in the center of the top of the connecting block in a penetrating mode, the fixing pin is movably arranged on one side of the mounting block, the external threads are formed in the outer peripheral face of one end of the fixing pin, and the connecting block is connected with the mounting block through the fixing pin.
Preferably, the sample pad all is the column structure of buckling with the cross section of colloidal gold pad, the one end bottom of sample pad and the top in close contact with of colloidal gold pad, one side in close contact with one side of NC membrane of colloidal gold pad.
Preferably, one side of the mounting block is provided with a through hole in a penetrating manner, one side of the connecting block is provided with a thread groove, the through hole and the thread groove are located on the same horizontal plane, the inner diameter of the through hole is larger than the diameter of the cross section of the fixing pin, and one end of the fixing pin is in threaded connection with the connecting block through an external thread.
Preferably, the number of the clamping plates is two, the two clamping plates are symmetrically arranged in the insertion groove, the height of each clamping plate is consistent with the depth of the inside of the insertion groove, and inclined plates are fixedly connected to the front end faces of the clamping plates.
Preferably, the top center department of testing panel has seted up the viewing aperture, the top of testing panel is kept away from one side of viewing aperture and is equipped with the dropping sample mouth, the dropping sample mouth is located the sample pad directly over.
Preferably, the top of backup pad is run through and is seted up the lofting hole, the lofting hole is located the dropping pattern mouth directly over, the lofting hole is located same vertical face with the dropping pattern mouth.
Preferably, one end of the test board is fixedly connected with a connecting plate, and the side-view cross-sectional dimension of the connecting plate is smaller than the inner dimension of the insertion groove.
Preferably, the test lines comprise a G test line and an M test line, the M test line and the G test line are positioned on the NC membrane, and the M test line and the G test line are sequentially arranged from left to right.
Preferably, the control line is coated with an anti-mouse IgG polyclonal antibody, the G detection line is coated with an anti-human IgG monoclonal antibody, and the M detection line is coated with an anti-human IgM-mu chain monoclonal antibody.
Preferably, the both ends of first spring are fixed in the inside of cavity and one side of limiting plate respectively, the both ends of second spring are fixed in the inner wall of lagging and the one end of dead lever respectively, the internal dimension of recess is greater than the cross sectional dimension of connecting block, the cross sectional dimension of hexagonal pole is less than the internal dimension of hexagonal hole.
Compared with the prior art, the invention has the following beneficial effects:
1. in the present invention, a fluorescence immunochromatography method is used through a test plate, and the principle of the chromatography is a separation technique established by using the differences in physical properties (such as adsorption force, molecular shape and size, molecular polarity, molecular affinity, partition coefficient, etc.) of each component in a mixture. The chromatographic system consists of a stationary phase and a mobile phase. The colloidal gold is filled up and is wrapped up novel coronavirus recombination antigen and mouse IgG of pre-coated gold mark, at NC membrane detection line and control line punishment do not wrap up anti human IgM-mu chain monoclonal antibody, anti human IgG antibody and anti mouse IgG antibody, use immunochromatography technique, detect human antigen qualitatively, can carry out the detection of antigen fast, guarantee the efficiency and the quality that new coronavirus antigen detected, the device can realize the popularization on basic level, can be applicable to the use of basic level middle and small hospital, and then promote the construction of whole novel coronavirus epidemic situation prevention and control system.
2. According to the invention, through the arranged clamping component, when antigen detection is required, the test board is taken out of the kit body and is placed into the insertion groove through the connecting plate, two sides of the connecting plate are in contact with the inclined plate to generate extrusion force to push the clamping plate to two sides, the sleeve plate extrudes the first spring to enable the first spring to be compressed on the outer peripheral surface of the fixed rod, meanwhile, the sleeve plate moves towards the inside of the cavity through the limiting plate, the end part of the fixed rod stretches out and extends from the inside of the sleeve plate, the second spring is compressed, then, after the connecting plate completely enters the insertion groove, the connecting plate is clamped and fixed through the restorability of the first spring and the second spring, so that the test board can be firmly clamped, the situation that the test board deviates during detection is avoided, and other risks of detection are reduced.
3. According to the invention, through the arranged installation assembly, the connecting block is firstly sleeved on the peripheral surface of the hexagonal rod through the hexagonal hole, the connecting block is completely placed at the bottom of the groove, then the fixing pin is rotated, the external thread arranged on the peripheral surface of one end of the fixing pin is rotated into the interior of the thread groove, and the fixing pin is screwed, so that the supporting plate is fixed on the installation block through the fixing pin, then the sample reagent tube is placed in the sample placing hole, the sample dropping hole and the sample dropping hole are positioned on the same vertical surface, the sample can be conveniently dropped for detection, excessive contact can be avoided, the sample detection is conveniently and quickly carried out, and the quality and the efficiency of antigen detection are improved.
Drawings
FIG. 1 is a schematic diagram of the overall three-dimensional structure of a detection kit for a new coronavirus antigen of the present invention;
FIG. 2 is a schematic view of a partial three-dimensional structure of a novel coronavirus antigen detection kit according to the present invention;
FIG. 3 is a schematic view of the overall three-dimensional structure of a test board of the reagent kit for detecting a new coronavirus antigen according to the present invention;
FIG. 4 is a schematic view of a partial three-dimensional structure of a test board of the reagent kit for detecting a new coronavirus antigen according to the present invention;
FIG. 5 is a schematic diagram of a side view and an internal cross-sectional structure of a fixed block of the reagent kit for detecting a new coronavirus antigen of the present invention;
FIG. 6 is an enlarged schematic view of the A position in FIG. 5 of the reagent kit for detecting a new coronavirus antigen according to the present invention;
FIG. 7 is a schematic view of a three-dimensional structure of a clamping plate of the reagent kit for detecting a new coronavirus antigen according to the present invention;
FIG. 8 is a schematic view of the three-dimensional structure of the mounting block of the reagent kit for detecting a new coronavirus antigen of the present invention;
FIG. 9 is a schematic diagram of a partial explosion structure of the detection kit for a new coronavirus antigen according to the present invention;
FIG. 10 is a schematic perspective view of a support plate of the detection kit for a new coronavirus antigen of the present invention.
In the figure: 1. a kit body; 2. a test board; 3. a fixed block; 4. mounting a block; 5. a support plate; 6. a PVC base pad; 7. a viewing port; 8. a sample dropping port; 9. NC film; 10. a test line; 11. a control line; 12. a sample pad; 13. a colloidal gold pad; 14. a water absorbent pad; 15. a connecting plate; 16. inserting the groove; 17. a cavity; 18. sheathing; 19. a limiting plate; 20. fixing the rod; 21. a first spring; 22. a second spring; 23. a clamping plate; 24. a sloping plate; 25. a groove; 26. a hexagonal bar; 27. connecting blocks; 28. a hexagonal hole; 29. a through hole; 30. a fixing pin; 31. an external thread; 32. a thread groove; 33. and (4) placing a sample hole.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-10, the invention is a new coronavirus antigen detection kit, comprising a kit main body 1, a test board 2 is arranged inside the kit main body 1, a fixed block 3 is arranged on one side of the top of the kit main body 1, the inside of the fixed block 3 is connected with the test board 2 through a clamping component, an installation block 4 is arranged on the top of the fixed block 3, and one side of the installation block 4 is connected with a support plate 5 through an installation component;
the test board 2 comprises a PVC base pad 6, an NC membrane 9, a test wire 10, a control wire 11, a sample pad 12, a colloidal gold pad 13 and a water absorption pad 14, the PVC base pad 6 is arranged at the bottom of the test board 2, the NC membrane 9 is fixedly connected to the center of the top of the PVC base pad 6, the test wire 10 and the control wire 11 are sequentially arranged at the top of the NC membrane 9 from left to right, the sample pad 12 is arranged on one side of the top of the PVC base pad 6, the colloidal gold pad 13 is connected to the bottom of one side of the sample pad 12, and the water absorption pad 14 is arranged on the other side of the top of the PVC base pad 6;
the clamping assembly comprises a sleeve plate 18, a limiting plate 19, a fixing rod 20, a first spring 21, a second spring 22 and a clamping plate 23, wherein an insertion groove 16 is formed in one side of the fixing block 3, two opposite inner walls in the insertion groove 16 are respectively provided with a cavity 17, the sleeve plate 18 is movably arranged in the cavity 17, one end of the sleeve plate 18 is fixedly connected with one side of the limiting plate 19, the fixing rod 20 is fixedly connected in the cavity 17, the first spring 21 is sleeved in the cavity 17 on the outer peripheral surface of the fixing rod 20, one end of the fixing rod 20 is fixedly connected in the sleeve plate 18, and one end of the sleeve plate 18 is fixedly connected with one side of the clamping plate 23;
the installation component comprises a hexagonal rod 26, a connecting block 27, a fixing pin 30 and external threads 31, a groove 25 is formed in one side of the top of the installation block 4, the hexagonal rod 26 is fixedly connected to the inside bottom plate of the groove 25 in the vertical direction, the one end of the supporting plate 5 is fixedly connected with one side of the connecting block 27, a hexagonal hole 28 is formed in the center of the top of the connecting block 27 in a penetrating mode, the fixing pin 30 is movably arranged on one side of the installation block 4, the external threads 31 are formed in the outer peripheral face of one end of the fixing pin 30, and the connecting block 27 is connected with the installation block 4 through the fixing pin 30.
The cross sections of the sample pad 12 and the colloidal gold pad 13 are both of a bent structure, the bottom of one end of the sample pad 12 is in close contact with the top of the colloidal gold pad 13, and one side of the colloidal gold pad 13 is in close contact with one side of the NC membrane 9.
One side of the mounting block 4 is provided with a through hole 29 in a penetrating manner, one side of the connecting block 27 is provided with a thread groove 32, the through hole 29 and the thread groove 32 are positioned on the same horizontal plane, the inner diameter of the through hole 29 is larger than the cross section diameter of the fixing pin 30, and one end of the fixing pin 30 is in threaded connection with the connecting block 27 through an external thread 31.
The number of the clamping plates 23 is two, the two clamping plates 23 are symmetrically arranged inside the insertion groove 16, the height of the clamping plates 23 is consistent with the depth inside the insertion groove 16, and the front end faces of the clamping plates 23 are fixedly connected with inclined plates 24.
The top of backup pad 5 is run through and has been seted up and has been put kind hole 33, puts kind hole 33 and is located dripping appearance mouth 8 directly over, and lofting hole 33 and dripping appearance mouth 8 are in same vertical face, avoid the contact too much, make things convenient for the supplementary drippage of sample to detect.
One end of the test board 2 is fixedly connected with a connection plate 15, and the side-view cross-sectional dimension of the connection plate 15 is smaller than the inner dimension of the insertion groove 16.
The test line 10 comprises a G test line and an M test line, the M test line and the G test line are located on the NC membrane 9, and the M test line and the G test line are sequentially arranged from left to right.
The control line 11 is coated with an anti-mouse IgG polyclonal antibody, the G detection line is coated with an anti-human IgG monoclonal antibody, and the M detection line is coated with an anti-human IgM-mu chain monoclonal antibody.
The two ends of the first spring 21 are respectively fixed inside the cavity 17 and one side of the limit plate 19, the two ends of the second spring 22 are respectively fixed on the inner wall of the sleeve plate 18 and one end of the fixing rod 20, the inner size of the groove 25 is larger than that of the connecting block 27, and the cross-sectional size of the hexagonal rod 26 is smaller than that of the hexagonal hole 28.
The working principle of the invention is as follows: when the test board 2 is used, the test board 2 is taken out of the kit body 1 and is placed into the insertion groove 16 through the connecting board 15, two sides of the connecting board 15 are in contact with the inclined board 24 to generate extrusion force to push the clamping board 23 to two sides, the sleeve board 18 extrudes the first spring 21 to compress the first spring 21 on the outer peripheral surface of the fixed rod 20, the sleeve board 18 moves towards the inside of the cavity 17 through the limiting board 19, the end part of the fixed rod 20 extends towards the inside of the sleeve board 18 to compress the second spring 22, then the connecting board 15 completely enters the insertion groove 16, the connecting board 15 is clamped and fixed through the restorability of the first spring 21 and the second spring 22, so that the test board 2 can be firmly clamped, then the connecting block 27 is sleeved on the outer peripheral surface of the hexagonal rod 26 through the hexagonal hole 28 and is completely placed at the bottom of the groove 25, then the fixing pin 30 is rotated, the external screw thread 31 that makes the one end peripheral face of fixed pin 30 set up changes the inside of thread groove 32, and screw up fixed pin 30, thereby make backup pad 5 be fixed in on installation piece 4 through fixed pin 30, then through putting into the inside of putting into appearance hole 33 with sample reagent pipe, be in same vertical face through putting out the appearance hole 33 and dropping out appearance mouth 8, can make things convenient for instiling into of sample to detect, the sample instils into through dropping out the appearance mouth 8, fall into on sample pad 12, and remove through the chromatography, novel coronavirus recombination antigen of gold mark and mouse IgG are wrapped up in advance on colloidal gold pad 13, test wire 10 and control line 11 punishment on NC membrane 9 are respectively peridium anti human IgM-mu chain monoclonal antibody, anti human IgG antibody and anti mouse IgG antibody, use immunochromatography, the new corona virus antigen in the qualitative detection human body.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A novel coronavirus antigen detection kit is characterized in that: the kit comprises a kit main body (1), wherein a test board (2) is arranged inside the kit main body (1), a fixed block (3) is arranged on one side of the top of the kit main body (1), the inside of the fixed block (3) is connected with the test board (2) through a clamping component, an installation block (4) is arranged on the top of the fixed block (3), and one side of the installation block (4) is connected with a support plate (5) through an installation component;
the test board (2) comprises a PVC base pad (6), an NC membrane (9), a test line (10), a control line (11), a sample pad (12), a colloidal gold pad (13) and a water absorption pad (14), the PVC base pad (6) is arranged at the bottom of the test board (2), the NC membrane (9) is fixedly connected to the center of the top of the PVC base pad (6), the test line (10) and the control line (11) are sequentially arranged at the top of the NC membrane (9) from left to right, the sample pad (12) is arranged on one side of the top of the PVC base pad (6), the colloidal gold pad (13) is connected to the bottom of one side of the sample pad (12), and the water absorption pad (14) is arranged on the other side of the top of the PVC base pad (6);
the clamping component comprises a sleeve plate (18), a limit plate (19), a fixed rod (20), a first spring (21), a second spring (22) and a clamping plate (23), one side of the fixed block (3) is provided with an insertion groove (16), two opposite inner walls inside the insertion groove (16) are both provided with a cavity (17), sleeve plates (18) are movably arranged in the cavities (17), one end of each sleeve plate (18) is fixedly connected with one side of a limiting plate (19), a fixed rod (20) is fixedly connected inside the cavity (17), a first spring (21) is sleeved on the outer peripheral surface of the fixed rod (20) and positioned inside the cavity (17), one end of the fixed rod (20) is positioned in the sleeve plate (18) and is fixedly connected with a second spring (22), one end of the sleeve plate (18) is fixedly connected with one side of the clamping plate (23);
the mounting assembly comprises a hexagonal rod (26), a connecting block (27), a fixing pin (30) and external threads (31), a groove (25) is formed in one side of the top of the mounting block (4), the hexagonal rod (26) is fixedly connected with the vertical direction of an internal bottom plate of the groove (25), one end of the supporting plate (5) is fixedly connected with one side of the connecting block (27), the top center of the connecting block (27) is provided with a hexagonal hole (28) in a penetrating mode, one side of the mounting block (4) is movably provided with the fixing pin (30), the external threads (31) are formed in the outer peripheral face of one end of the fixing pin (30), and the connecting block (27) is connected with the mounting block (4) through the fixing pin (30).
2. The kit for detecting a neocoronavirus antigen according to claim 1, wherein the kit comprises: the cross section of sample pad (12) and colloidal gold pad (13) all is the column structure of buckling, the top in close contact with of the one end bottom of sample pad (12) and colloidal gold pad (13), one side in close contact with of one side of colloidal gold pad (13) and NC membrane (9).
3. The kit for detecting a neocoronavirus antigen according to claim 1, wherein the kit comprises: one side of the mounting block (4) is penetrated and provided with a through hole (29), one side of the connecting block (27) is provided with a thread groove (32), the through hole (29) and the thread groove (32) are positioned on the same horizontal plane, the inner diameter of the through hole (29) is larger than the cross section diameter of the fixing pin (30), and one end of the fixing pin (30) is in threaded connection with the connecting block (27) through an external thread (31).
4. The kit for detecting a neocoronavirus antigen according to claim 1, wherein the kit comprises: the number of the clamping plates (23) is two, the two clamping plates (23) are symmetrically arranged inside the insertion groove (16), the height of the clamping plates (23) is consistent with the depth inside the insertion groove (16), and the front end faces of the clamping plates (23) are fixedly connected with inclined plates (24).
5. The kit for detecting a neocoronavirus antigen according to claim 1, wherein the kit comprises: survey the top center department of testing panel (2) and seted up viewing aperture (7), the top of testing panel (2) is kept away from one side of viewing aperture (7) and is equipped with and drips appearance mouth (8), drip appearance mouth (8) are located sample pad (12) directly over.
6. The kit for detecting the antigen of a new coronavirus according to claim 5, wherein: the top of backup pad (5) is run through and has been seted up and has been put appearance hole (33), put appearance hole (33) and be located directly over dripping appearance mouth (8), put appearance hole (33) and drip appearance mouth (8) and be in on the same vertical face.
7. The kit for detecting a neocoronavirus antigen according to claim 1, wherein the kit comprises: one end of the test board (2) is fixedly connected with a connecting plate (15), and the side-view cross section size of the connecting plate (15) is smaller than the inner size of the insertion groove (16).
8. The kit for detecting a neocoronavirus antigen according to claim 1, wherein the kit comprises: the test line (10) comprises a G test line and an M test line, the M test line and the G test line are located on the NC membrane (9), and the M test line and the G test line are sequentially arranged from left to right.
9. The kit for detecting the antigen of a new coronavirus according to claim 1, wherein: the control line (11) is coated with an anti-mouse IgG polyclonal antibody, the G detection line is coated with an anti-human IgG monoclonal antibody, and the M detection line is coated with an anti-human IgM-mu chain monoclonal antibody.
10. The kit for detecting a neocoronavirus antigen according to claim 1, wherein the kit comprises: the both ends of first spring (21) are fixed in the inside of cavity (17) and one side of limiting plate (19) respectively, the both ends of second spring (22) are fixed in the inner wall of lagging (18) and the one end of dead lever (20) respectively, the inside dimension of recess (25) is greater than the cross sectional dimension of connecting block (27), the cross sectional dimension of hexagonal pole (26) is less than the inside dimension of hexagonal hole (28).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210582441.1A CN114966017A (en) | 2022-05-26 | 2022-05-26 | New coronavirus antigen detection kit based on fluorescence immunochromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210582441.1A CN114966017A (en) | 2022-05-26 | 2022-05-26 | New coronavirus antigen detection kit based on fluorescence immunochromatography |
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| Publication Number | Publication Date |
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| CN114966017A true CN114966017A (en) | 2022-08-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210582441.1A Pending CN114966017A (en) | 2022-05-26 | 2022-05-26 | New coronavirus antigen detection kit based on fluorescence immunochromatography |
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| WO2021159703A1 (en) * | 2020-02-13 | 2021-08-19 | 北京华科泰生物技术股份有限公司 | Immunochromatographic kit for rapidly detecting novel coronavirus n protein, and preparation method and application thereof |
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