CN114965846A - Method for measuring nitrogen content of moso bamboo leaves in unit area by using quality of moso bamboo leaves - Google Patents
Method for measuring nitrogen content of moso bamboo leaves in unit area by using quality of moso bamboo leaves Download PDFInfo
- Publication number
- CN114965846A CN114965846A CN202210534352.XA CN202210534352A CN114965846A CN 114965846 A CN114965846 A CN 114965846A CN 202210534352 A CN202210534352 A CN 202210534352A CN 114965846 A CN114965846 A CN 114965846A
- Authority
- CN
- China
- Prior art keywords
- moso bamboo
- leaves
- bamboo leaves
- nitrogen content
- mass
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/002—Determining nitrogen by transformation into ammonia, e.g. KJELDAHL method
Abstract
The invention provides a method for measuring the nitrogen content of moso bamboo leaves in unit area by using the mass of the moso bamboo leaves, belonging to the technical field of plant element measurement. The method comprises the steps of measuring the dry weight and the leaf area of the moso bamboo leaves to obtain the specific leaf mass of the moso bamboo; measuring the nitrogen content of the moso bamboo leaves in unit mass, and substituting the nitrogen content into a formula to obtain the nitrogen content of the moso bamboo leaves in unit area; or substituting the mass of the specific leaves into a formula to obtain the nitrogen content of the moso bamboo leaves in unit area, wherein the formula is as follows: y 0.0223x + 0.0577. The method provided by the invention is environment-friendly, low in cost, simple to operate and capable of rapidly determining the nitrogen content of the moso bamboo leaves in unit area.
Description
Technical Field
The invention relates to the technical field of plant element determination, in particular to a method for determining the nitrogen content of moso bamboo leaves in unit area by using the mass of moso bamboo leaves.
Background
Phyllostachys pubescens (Phyllostachys pubescens) has the advantages of fast growth, early yield, high yield, wide application, high income and the like, is an important forest resource in southern China, and is an increasing carbon sink because the carbon fixing capability of the Phyllostachys pubescens is far superior to that of common forest trees. In recent years, with the implementation of beneficial forestry, about 40% of moso bamboo forests realize intensive operation, and the change of the operating mode of the moso bamboo forests can generate negative influence on the output process of soil carbon. How to more effectively and scientifically rationalize the existing bamboo forest, reduce the environmental pollution caused by the use of a large amount of nitrogen fertilizer and other compound fertilizers and reduce the degradation of the bamboo forest land caused by excessive investment, how to reduce the use of the nitrogen fertilizer on the premise of increasing the biomass accumulation and carbon assimilation of the bamboo forest, realize 'win-win' and is particularly important for further improving the carbon fixation and sink increase capacity of the ecological system of the bamboo forest. Due to the fact that global change is aggravated, the occurrence frequency and the intensity of extreme climatic events are increased, and the problem of how to solve the problem of cooperative development of the green environmental protection technology for intensive management of the moso bamboo forest and scientific use of nitrogen fertilizers under the background of climate change is particularly urgent.
Leaves, as an important photosynthetic organ, are the major site of plant photosynthetic product formation. The ratio of the dry matter of a blade to its blade area is defined as the specific blade mass (LMA), which can be understood as the cost of trapping light at the blade level. The specific leaf quality is one of the key leaf characters of the plant leaf, is an important characteristic of plant growth, and is also an important index of the growth strategy which is widely applied to plant ecology, agriculture and forestry and comprehensively reflects the resource utilization capability of the plant and adapts to the environment.
Nitrogen is an essential nutrient element for plant growth and development and an important limiting factor for the productivity of forest ecosystems. The vertical gradient distribution of the nitrogen content of the canopy leaves is one of the important and common characteristics of the forest canopy. The maximum photosynthetic rate of trees is closely related to leaf nitrogen content and light radiation, and the gradient change of the vertical distribution of leaf nitrogen in unit area is regarded as the plastic reaction for optimizing the nitrogen utilization of plants in the carbon assimilation process. In general, leaves under higher light conditions have a greater specific leaf mass, nitrogen per leaf area and photosynthetic capacity per area than leaves in a shaded environment.
The technical field of judging the nitrogen and nutrient contents of the leaves in the existing bamboo forest production and operation lacks a practice method which is environment-friendly, low in cost, convenient and easy to operate, and particularly lacks a method for quickly judging the nitrogen content of the leaves based on the key character variation of the leaves.
Disclosure of Invention
In view of this, the invention aims to provide a method for measuring the nitrogen content of the moso bamboo leaves in unit area by using the specific leaf mass.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for measuring the nitrogen content of moso bamboo leaves in unit area by using the mass of moso bamboo leaves, which comprises the following steps:
measuring the dry weight and the leaf area of the moso bamboo leaves to obtain the specific leaf mass of the moso bamboo; the unit of the specific leaf mass is g/m 2 ；
Substituting the mass of the specific leaves into a formula to obtain the nitrogen content of the moso bamboo leaves in unit area, wherein the formula is as follows: y is 0.0223x +0.0577, wherein y is the nitrogen content of the moso bamboo leaves in unit area and the unit is g/m 2 (ii) a And x is the specific leaf mass.
Preferably, 10-15 moso bamboo leaves are taken to measure dry weight and leaf area, and the average value is taken to calculate specific leaf mass.
Preferably, the leaf area of the moso bamboo is obtained by adopting WinRHIO software scanning processing.
Preferably, the moso bamboo leaves are dried to constant weight at 80 ℃ to obtain the dry weight of the moso bamboo leaves.
Preferably, the nitrogen content of the moso bamboo leaves in unit mass is determined by adopting a Kjeldahl method.
Preferably, the moso bamboo leaves are harvested in sunny, cloudless weather.
The invention has the beneficial effects that:
the method provided by the invention can be used for rapidly, environmentally and cheaply judging the nitrogen content of the moso bamboo leaves in unit area. And the nitrogen nutrition condition of the individual phyllostachys pubescens and the season and space distribution pattern thereof are evaluated through the nitrogen content of unit areas of 30-50 leaves, so that the forest stand fertilization management can be indirectly guided. The method provided by the invention does not need chemical reagents and is convenient and simple to operate.
In this application, nitrogen is an important limiting factor for the productivity of the phyllostachys edulis ecosystem. Nitrogen content per unit area of moso bamboo leaves (N) area ) Varying with canopy height and gradient distribution affected by the growing season. Nitrogen content (N) of Phyllostachys Pubescens leaves in spring and summer area ) Increasing with increasing canopy height. Existence of vertical distribution gradient of bamboo leaf nitrogen and variation of the distribution gradient along with growing seasons. Leaf of moso bambooMass (LMA) and N area The correlation of (A) is very obvious, the moso bamboo leaves N area The crown gradient distribution of the LMA shows similar change trend, which shows that the crown gradient distribution of the LMA of the phyllostachys pubescens leaf can indirectly reflect N thereof area Distribution tendency of (a). The method provided by the invention provides a theoretical basis for sustainable and efficient operation of the bamboo artificial forest under the global climate background.
Detailed Description
The invention provides a method for measuring the nitrogen content of moso bamboo leaves in unit area by using the mass of moso bamboo leaves, which comprises the following steps:
measuring the dry weight and the leaf area of the moso bamboo leaves to obtain the specific leaf mass of the moso bamboo; the unit of the specific leaf mass is g/m 2 ；
Substituting the mass of the specific leaves into a formula to obtain the nitrogen content of the moso bamboo leaves in unit area, wherein the formula is as follows: y is 0.0223x +0.0577, wherein y is the nitrogen content of the moso bamboo leaves in unit area and the unit is g/m 2 (ii) a x is the specific leaf mass.
The dry weight and the leaf area of 10-15 moso bamboo leaves are preferably measured, and the average value is taken to calculate the specific leaf mass. The area of the moso bamboo leaves is preferably obtained by adopting WinRHIO (Pro2007) software scanning processing. According to the invention, the moso bamboo leaves are preferably dried to constant weight at 80 ℃ to obtain the dry weight of the moso bamboo leaves, and the dry weight of the moso bamboo leaves is preferably weighed by using an electronic balance (with the accuracy of 0.001 g). The moso bamboo leaves are preferably obtained in sunny and cloudy weather, the obtaining mode is not particularly limited, and the method can be conveniently used by a person skilled in the art.
In order to further illustrate the present invention, the following detailed description of the invention is given in conjunction with examples, which should not be construed as limiting the scope of the invention.
Example 1
The implementation place is as follows: the Temple mountain depressed forest farm mao bamboo forest in Huanggong forest park of Zhejiang province is located in the sunny area of the West outskirt of Hangzhou (119 degrees 56 '-120 degrees 02' E, 30 degrees 03 '-30 degrees 06' N).
Area of test area: 15 hectares;
topography: belonging to the afterpulse of Tianmu mountain system in the low hilly area of Zhejiang West;
climate: belonging to the subtropical monsoon climate zone;
the slope direction: a south slope, a south east slope, and an east slope;
gradient: 18-20 degrees;
altitude: 150m-220 m;
annual average rainfall: 1441.9 mm;
average annual temperature: 16.1 ℃ (the highest temperature is 40.2 ℃, and the lowest temperature is-14.4 ℃);
the soil belongs to acid red soil;
the area is clear in four seasons, the climate is humid, and the illumination is sufficient.
The phyllostachys pubescens forest in the test area is planted in the 60 th generation of the 20 th century and is a natural renewal forest in extensive operation. The density of the moso bamboo forest is 3875 strains per hm -2 The canopy density is 0.95, the breast diameter of the bamboo plants is distributed between 4.0 and 13.6cm, the average breast diameter is 9.8cm, and the average bamboo height is 13.2 m. Under forest there are almost no shrubs and herbs, but the ground surface is covered with litter of about 2.0cm in average thickness. The method is used for 1 time of mountain splitting every 2 years, no fertilizer is applied, no tillage is carried out, and only the spring bamboo shoots are harvested.
(1) Leaf collection and related index determination: and selecting clear and cloudless weather samples in middle and late days of each month in 2015 and 2016 respectively to continuously measure indexes such as leaf nitrogen, specific leaf quality and the like. 2 moso bamboos with the same growth vigor and similar breast diameter sizes and different ages are respectively selected in each sample land. With the help of a ladder temporarily built in the field, respectively aiming at the sunward positions of the upper, middle and lower canopy parts of moso bamboos of each bamboo age, hooking the sunward branches by using a sickle, rapidly picking all the leaves and taking the leaves back to a laboratory for measuring the leaf specific mass and the nutrient content of the leaves.
(2) Determining the specific leaf mass of the leaves: respectively taking 10-15 leaves from the bamboo age and the canopy to determine the specific leaf mass, repeatedly determining for 3 times, and finally taking an average value. The leaf area was obtained using a WinRHIZO (Pro2007) software scan process. The sampled leaves were baked at 80 ℃ for 24h to constant mass, their dry mass was weighed with an electronic balance (precision 0.001g), and the specific leaf mass was calculated: LMA (g.m) -2 ) Dry mass of the blade/area of the blade.
(3) Leaf nitrogen content determination: drying the residual leaf sample by using an oven(same manner as above) and ground through a 100 mesh sieve for leaf nitrogen content determination. The content of leaf nitrogen is determined by Foss Kjeldahl azotometer, and digestion solution obtained by digesting a sample with concentrated sulfuric acid-hydrogen peroxide solution is used for total nitrogen (N) mass ) And (4) measuring.
(4) Based on the data set, fitting and establishing the nitrogen content (y, g.m) of the moso bamboo leaves in unit area by adopting a linear regression analysis method -2 ) And the mass of leaf of Hebia (x, g.m) -2 ) Linear correlation equation of the two: 0.0223x +0.0577, R 2 ＝0.8575。
Example 2
The implementation place is as follows: mao bamboo forest in Jianglang mountain natural protection area of Zhejiang province;
area of test area: 15 hectare;
slope position: a north slope, a south-east slope;
gradient: 25-28 degrees;
altitude: 400m-1500 m;
annual average rainfall: 1820 mm;
annual average temperature: 17.1 ℃;
the area is clear in four seasons, the climate is humid, and the illumination is sufficient.
Vegetation composition conditions of the protected area: the forest coverage rate reaches 67.1%, and natural vegetation comprises 4 groups of evergreen broad-leaved forest, coniferous forest and shrub, 7 classes and 15 group systems.
Below the elevation of 900m, there are mostly woody lotus (Schima superba) forest, masson pine (Pinus massoniana) forest, Cunninghamia lanifolia forest, Phyllostachys pubescens (Phyllostachys pubescens) forest, secondary shrubs and other vegetation types.
The test determines the tree species: individuals of different ages in a Phyllostachys pubescens (Phyllostachys pubescens) forest;
the determination method comprises the following steps: and in 2018, 2020, 8 and 11 months, respectively selecting clear weather, and collecting 20-30 leaves at the middle and lower parts of the canopy of the individual moso bamboos of different ages of the naturally distributed moso bamboo forest in the natural protection area of the Jianlangshan mountain. The leaves were packed in plastic bags and quickly brought back to the room for determination of leaf ratio quality in the same manner as in example 1.
Obtaining specific leaf quality data sets of the individual leaves of the phyllostachys pubescens forest at different ages in the protection area, and adopting a linear correlation equation of the specific leaf quality data sets and the linear correlation equation of the specific leaf quality data sets: and y is 0.0223x +0.0577, the nitrogen content data set of the unit area of the individual leaf of the moso bamboo in the area is measured, the nutrient condition of the moso bamboo forest is known in time, and the production and management of the moso bamboo forest in the area are guided, namely tending management comprises nutrient management.
The average specific leaf mass of the moso bamboo forest leaves in the area is 38.6 g.m -2 The average unit area of the leaf blade nitrogen content is 0.92 g.m -2 。
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (6)
1. A method for measuring the nitrogen content of a moso bamboo leaf in unit area by using the mass of a leaf, which is characterized by comprising the following steps:
measuring the dry weight and the leaf area of the moso bamboo leaves to obtain the specific leaf mass of the moso bamboo; the unit of the specific leaf mass is g/m 2 ；
Substituting the mass of the specific leaves into a formula to obtain the nitrogen content of the moso bamboo leaves in unit area, wherein the formula is as follows: y is 0.0223x +0.0577, wherein y is the nitrogen content of the moso bamboo leaves in unit area and the unit is g/m 2 (ii) a x is the specific leaf mass.
2. The method according to claim 1, wherein the dry weight and the leaf area of 10 to 15 moso bamboo leaves are measured, and the average value is used to calculate the specific leaf mass.
3. The method of claim 1 or 2, wherein the area of the moso bamboo leaves is obtained by scanning with WinRHIO software.
4. The method according to claim 1 or 2, wherein the moso bamboo leaves are dried to a constant weight at 80 ℃ to obtain a dry weight of the moso bamboo leaves.
5. The method according to claim 1, wherein the nitrogen content per unit mass of the phyllostachys pubescens leaves is measured by kjeldahl method.
6. The method according to claim 1, wherein the moso bamboo leaves are harvested on a sunny and cloudless day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210534352.XA CN114965846A (en) | 2022-05-17 | 2022-05-17 | Method for measuring nitrogen content of moso bamboo leaves in unit area by using quality of moso bamboo leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210534352.XA CN114965846A (en) | 2022-05-17 | 2022-05-17 | Method for measuring nitrogen content of moso bamboo leaves in unit area by using quality of moso bamboo leaves |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114965846A true CN114965846A (en) | 2022-08-30 |
Family
ID=82984244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210534352.XA Pending CN114965846A (en) | 2022-05-17 | 2022-05-17 | Method for measuring nitrogen content of moso bamboo leaves in unit area by using quality of moso bamboo leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114965846A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080239293A1 (en) * | 2005-11-21 | 2008-10-02 | Fuchigami Leslie H | Portable Meter to Measure Chlorophyll, Nitrogen and Water and Methods |
| CN103868880A (en) * | 2014-01-24 | 2014-06-18 | 河南农业大学 | Wheat leaf nitrogen content monitoring method based on spectrum double-peak index and method for establishing monitoring model |
| CN114047139A (en) * | 2021-11-10 | 2022-02-15 | 四川省农业科学院园艺研究所 | Standard curve color card for representing different nitrogen concentrations of leaves and preparation method and application thereof |
-
2022
- 2022-05-17 CN CN202210534352.XA patent/CN114965846A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080239293A1 (en) * | 2005-11-21 | 2008-10-02 | Fuchigami Leslie H | Portable Meter to Measure Chlorophyll, Nitrogen and Water and Methods |
| CN103868880A (en) * | 2014-01-24 | 2014-06-18 | 河南农业大学 | Wheat leaf nitrogen content monitoring method based on spectrum double-peak index and method for establishing monitoring model |
| CN114047139A (en) * | 2021-11-10 | 2022-02-15 | 四川省农业科学院园艺研究所 | Standard curve color card for representing different nitrogen concentrations of leaves and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 黎莉等: "三江湿地典型植被的叶片氮含量与比叶面积关系的研究" * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101884278A (en) | Method for improving activity of protective enzyme of festuca arundinacea under drought conditions | |
| Devakumar et al. | Drought alters the canopy architecture and micro-climate of Hevea brasiliensis trees | |
| Mehta et al. | Socio-economic impact of protected cultivation on tomato growers of Himachal Pradesh | |
| Stephens | Productivity of red pine, 1. Foliage distribution in tree crown and stand canopy | |
| Naher et al. | Effect of long-term nutrient management on soil organic carbon sequestration in rice–rice–fallow rotation | |
| CN109479668B (en) | Light matrix for qiongzhuea tumidinoda container seedling raising | |
| CN106053447B (en) | Method for measuring soil carbon sequestration effect in rice growing season and biochar composition | |
| CN107056364A (en) | A kind of apple nursery stock nursery container for plant growth and fertilizer | |
| CN102165865B (en) | Method for determining decomposition degree of organic fertilizers in municipal sludge by utilizing mainland vegetable seeds | |
| Hjelm et al. | The demand of hybrid aspen (Populus tremula× P. tremuloides) on site conditions for a successful establishment on forest land | |
| MirseyedHosseini et al. | Evaluation of different growth media for tomato seedlings to optimize production and water use | |
| CN102511325A (en) | Method for sieving salt-tolerant varieties of osmanthus fragrans | |
| CN110683920A (en) | Rhododendron growth promoting carbon-based liquid fertilizer and preparation method and application thereof | |
| CN116784158A (en) | Method for screening drought-resistant arundo donax by drought rehydration technology | |
| Collins et al. | Intercropping switchgrass with hybrid poplar increased carbon sequestration on a sand soil | |
| CN106489630A (en) | Degradable biological base fluid body plastic film for agricultural use and its production method | |
| CN114965846A (en) | Method for measuring nitrogen content of moso bamboo leaves in unit area by using quality of moso bamboo leaves | |
| Hytönen et al. | Biomass equations for rubber tree (Hevea brasiliensis) components in Southern Thailand | |
| Gyuleva et al. | Five years growth of Paulownia on two sites in Bulgaria. | |
| CN113951135A (en) | Screening method of saline-alkali tolerant plants | |
| CN109601219B (en) | Method for comprehensively evaluating drought resistance of Minjiang juniper in seedling stage | |
| Cheng et al. | A photosynthesis continuous monitoring system for CAM plants | |
| Abdolahipour et al. | Effect of time and amount of supplemental irrigation at different distances from tree trunks on quantity and quality of rain-fed fig production | |
| Han et al. | Effects of nitrogen application on growth and yield of maize | |
| Woldeamanual et al. | Assessment of Eucalyptus Globulus Coppice Yield in the Highland Areas of North Shewa, Ethiopia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |