CN114965796A - Method for analyzing irradiated mutton metabonomics by ultra-high performance liquid chromatography tandem mass spectrometry - Google Patents
Method for analyzing irradiated mutton metabonomics by ultra-high performance liquid chromatography tandem mass spectrometry Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/50—Conditioning of the sorbent material or stationary liquid
- G01N30/52—Physical parameters
- G01N30/54—Temperature
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
Abstract
The invention discloses a method for analyzing metabonomics of irradiated mutton by ultra-high performance liquid chromatography-tandem mass spectrometry, which comprises the steps of extracting mutton samples with different storage times and different irradiation doses by using an organic solvent, homogenizing, grinding, performing ultrasonic treatment, incubating, centrifuging and drying to obtain a dried substance, and performing redissolution, ultrasonic treatment and centrifugation on the dried substance to obtain supernatants of the mutton samples with different storage times and different irradiation doses; respectively measuring the supernatants of the mutton samples with different storage times and different irradiation doses by using a liquid chromatography-tandem mass spectrometry method, processing the obtained chromatographic data, adopting multivariate statistical analysis, namely unsupervised principal component analysis and supervised partial least square-discriminant analysis, and screening by taking the variable importance value greater than 1 as a standard to obtain the differential metabolic compounds of the mutton samples with different storage times and different irradiation doses. The method is simple and convenient to operate, time-saving and labor-saving, has high detection sensitivity, and provides a new idea for researching meat quality change.
Description
Technical Field
The invention belongs to the technical field of meat quality change supervision, and particularly relates to a method for analyzing irradiated mutton metabonomics by ultra-high performance liquid chromatography-tandem mass spectrometry.
Background
The quality of meat is a major characteristic that determines consumer acceptance and buying intent. The quality of meat can be further understood by analyzing the components of protein, lipid, peptide, small molecule metabolite and the like in the meat product. Non-volatile substances such as nucleotides and amino acids in fresh meat are critical to flavor precursors and affect the mouthfeel, tenderness and water retention capacity of meat and meat products. Metabolomics is a high-throughput method for analyzing endogenous metabolites in complex biological systems, and can identify and classify a large number of metabolites, pay attention to significant metabolic pathways involved by the metabolites, establish metabolic networks and speculate functions of the metabolites. LC-MS based metabolomics can explore the quality changes of meat by analyzing metabolites and metabolic profiles in meat.
Mutton is an important source for protein and other nutrient intake, and microbial pollution is easily caused in the slaughtering process. Irradiation is a green sterilization technology, the food safety can be guaranteed by irradiation dose treatment of less than 10kGy, the quality guarantee period of meat and meat products is prolonged, but the physicochemical properties of the meat are changed to different degrees while the irradiation sterilization is carried out, and the nutritional value and the sensory properties of the meat are influenced. At present, the research on the influence of irradiation on mutton mainly focuses on the measurement of physical and chemical indexes, and the research on the comprehensive metabolic spectrum difference and the metabolite change of the mutton in the irradiation process is neglected.
Therefore, it is necessary to develop a method for analyzing irradiated mutton by ultra performance liquid chromatography-tandem mass spectrometry.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a method for identifying irradiated mutton by using ultra-high performance liquid chromatography-tandem mass spectrometry, so as to solve the problems that the research on the influence of irradiation on mutton is mainly focused on the measurement of physicochemical indexes, and the comprehensive metabolic spectrum difference and the metabolite change of the mutton in the irradiation process are ignored.
In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose:
the invention discloses a method for analyzing irradiated mutton metabonomics by ultra-high performance liquid chromatography-tandem mass spectrometry, which comprises the following steps:
1) extracting an irradiated mutton sample to be detected by using an organic solvent, homogenizing, grinding, ultrasonically treating, incubating, centrifuging and drying to obtain a dried substance, and then re-dissolving, ultrasonically treating and centrifuging the dried substance to obtain a supernatant of the irradiated mutton sample to be detected;
2) determining the supernatant of the irradiated mutton sample to be detected by adopting a liquid chromatogram-tandem mass spectrometry, carrying out retention time comparison, peak extraction and integration, characteristic peak detection, background drift correction, database matching and data visualization processing on the obtained chromatographic data, carrying out unsupervised principal component analysis and supervised partial least square-discriminant analysis on the obtained result data set, screening to obtain a difference variable, obtaining a difference metabolite of the irradiated mutton sample to be detected, and establishing an integral metabolic spectrum of the irradiated mutton.
Preferably, in step 1, the organic solvent is a mixed solvent of methanol/acetonitrile/water in a volume ratio of 2:2: 1.
Preferably, in step 1, the storage time of the irradiated mutton sample to be detected is 0, 30 and 60 days, and the irradiation dose is 0, 1, 2, 4 and 6 kGy.
Preferably, in step 1, the grinding time is 5min, vortexing for 30s, sonication time is 10min, incubation at-20 ℃ for 20min, centrifugation at 10000 Xg for 20min, and centrifugation at 10000 Xg for 15min after reconstitution.
Preferably, in step 2, the supernatant of the irradiated mutton sample to be detected is filtered by a 0.22 μm membrane before being measured.
Preferably, in step 2, the liquid chromatography conditions are: hypersil Gold chromatographic column, mobile phase A is 0.1% formic acid in water, mobile phase B is 0.1% formic acid in acetonitrile; the column temperature is 30 ℃, the sample injection volume is 5 mu L, and the flow rate is 0.3 mL/min; the elution gradient was: 0-1 min, 15% B, 1-7 min, 15-40% B, 7-9 min, 40-70% B, 9-14 min, 70-100% B, 14-17 min, 100% B, 17-17.1 min, 100-15% B, 17.1-20 min, 15% B.
Preferably, in step 2, the mass spectrometry conditions are: the ion source is an electrospray ionization source and adopts a positive ion and negative ion detection mode; the flow rate of the sheath gas is 48-50 arb; the flow rate of the auxiliary gas is 11-15 arb; the temperature of the capillary tube is 300-350 ℃; the heating temperature of the auxiliary gas is 360-400 ℃.
Preferably, the mass spectrum uses Full MS and dd-MS 2 In the scanning mode, in the Full MS scanning mode, the scanning range m/z is 100-1500, the Full MS scanning resolution is 70000FWHM, and the AGC is 1e 6 (ii) a At dd-MS 2 In the scanning mode, the mass resolution is 17500FWHM and the AGC is 2e 5 。
Preferably, screening to obtain a difference variable by taking the variable importance value greater than 1 as a standard, and obtaining the difference metabolite of the mutton sample to be detected.
Preferably, the resulting differential metabolite data are subjected to analysis of variance at the 0.05 probability level.
Compared with the prior art, the invention has the following beneficial effects:
the invention discloses a method for analyzing irradiated mutton metabonomics by ultra-high performance liquid chromatography-tandem mass spectrometry, which utilizes the characteristics of ultra-high performance liquid chromatography, such as high separation degree, high sensitivity and easy combination with high resolution mass spectrometry, establishing an integral metabolic spectrum of mutton by using a metabonomics technology of a liquid chromatography-tandem mass spectrometry, screening differential metabolites contributing to differences among samples by combining multivariate statistical analysis, monitoring changes of key metabolites related to irradiation, explaining the change trend of the metabolites of the irradiated mutton, and determining key metabolic pathways influencing the quality of the irradiated mutton, meanwhile, the trend change of the storage time after irradiation treatment on the metabolites is analyzed, a powerful guarantee is provided for meat quality change supervision, a basis is provided for further research on the influence of irradiation on mutton quality, and a new visual angle is provided for research on irradiated mutton metabolic spectra. The method is simple and convenient to operate, time-saving and labor-saving, has high detection sensitivity, and provides a new idea for researching meat quality change.
Furthermore, under the condition of liquid chromatography, the separation degree and the peak type of each compound in the supernatant can be good, and the method is suitable for the subsequent metabonomics research of the irradiated mutton; under the condition of mass spectrum, good spectrum peak intensity and mass spectrum can be obtained, and qualitative and quantitative analysis of subsequent characteristic compounds is facilitated.
Further, variance analysis on the obtained characteristic compound data on the 0.05 probability level can be carried out, so that the different metabolic compounds of the irradiated mutton sample to be detected with obvious difference can be obtained, and the analysis of the irradiated mutton metabonomics can be better carried out.
Drawings
FIG. 1 is a flow chart of a meat quality change study method in a mutton irradiation process based on metabonomics according to the present invention;
FIG. 2 is a chart showing the influence of the content change of the differential metabolic compounds in irradiated mutton under positive and negative modes;
FIG. 3 is a graph showing the variation of the metabolites affecting meat quality according to the present invention over different storage times.
Detailed Description
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, shall fall within the protection scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or described herein. Moreover, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
1. Instrument for measuring the position of a moving object
An ultramate 3000 series liquid chromatography-Q-Orbitrap mass spectrometer (Thermo fisher scientific, usa), a vacuum nitrogen blow dryer (Caliper, usa), an Allegra-64R type high-speed refrigerated centrifuge (Beckman Coulter, usa), a Milli-Q Advantage type pure water instrument (Millipore, usa), a Vortex mixer (maritime, linbel instruments, inc.), a KQ5200E ultrasonic cleaner (jiangsu kunshan ultrasonic instruments, bas), a Tiss-12 type tissue disruptor (tuo he electromechanical technology, inc.).
2. Sample (I)
Mutton samples were irradiated at doses of 0, 1, 2, 4, 6kGy for different storage times (0, 30, 60 days).
3. Reagent
Formic acid, methanol and acetonitrile (Thermo fisher scientific, USA, pure chromatography)
4. Sample pretreatment
Weighing 0.1g mutton samples with different storage time and different irradiation doses, adding 1.5mL precooled mixed extract of methanol/acetonitrile/water with the ratio of 2:2:1, homogenizing, and grinding for 5 min. The mixture was vortexed for 30s and sonicated in an ice bath for 10 min. Thereafter, the sample was incubated at-20 ℃ for 20 minutes to precipitate the protein, and centrifuged at 10000 Xg for 20min at 4 ℃. The supernatant was transferred to a new sample tube and blown near dry using a nitrogen blow dryer. Then, the dried sample was redissolved by adding a 2:2:1 mixed extract of methanol/acetonitrile/water, sonicated in an ice bath for 10min, and centrifuged at 10000 Xg for 15 min. Finally, the supernatant was filtered through a 0.22 μm membrane and subjected to liquid chromatography.
5. Ultra-high performance liquid chromatography-quadrupole rod electrostatic field orbit ion trap mass spectrometry conditions
Non-targeted metabolomics analysis was performed by a UHPLC Q-Orbitrap system equipped with a heated electrospray ionization (HESI) source, with data acquisition in positive and negative ion mode. Chromatographic separation was done on a Hypersil Gold column (100 mm. times.2.1 mm, 5 μm) using an Ultimate3000UHPLC system. Mobile phase a was 0.1% formic acid in water and mobile phase B was 0.1% formic acid in acetonitrile. The column temperature was 30 ℃, the injection volume was 5 μ L, and the flow rate was 0.3 mL/min. The elution gradient was: 0-1 min, 15% B, 1-7 min, 15-40% B, 7-9 min, 40-70% B, 9-14 min, 70-100% B, 14-17 min, 100% B, 17-17.1 min, 100-15% B, 17.1-20 min, 15% B. Quality control sample (QC) random arrays were then prepared to monitor instrument stability throughout the analysis.
A Q-Orbitrap system with a heating electrospray ionization (HESI) source is used for data acquisition in a positive ion mode and a negative ion mode, and the capillary voltages in the acquisition modes are respectively 3.5kV and 3.2 kV. The detailed ion source parameters are as follows: the flow rate of the sheath gas is 48-50 arb; the flow rate of the auxiliary gas is 11-15 arb; the temperature of the capillary tube is 300-350 ℃; the heating temperature of the auxiliary gas is 360-400 ℃. By Full MS and dd-MS 2 And (5) analyzing the acquisition mode. In Full MS/dd-MS 2 In the mode, the scanning range (m/z) is 100-1500. Full MS scanning resolution is 70000FWHM and AGC is 1e 6 。dd-MS 2 Scan, Mass resolution 17500FWHM, AGC 2e 5 。
6. Data processing
Screening and establishing a detection result, and respectively detecting the supernatants of the mutton samples with different storage times and different irradiation doses by adopting a liquid chromatography-tandem mass spectrometry method to obtain total ion current chromatograms of the mutton samples with different storage times and different irradiation doses; and comparing the retention time of the obtained chromatographic data, extracting and integrating peaks, detecting characteristic peaks, correcting background drift, matching a database and visualizing the data to obtain a characteristic substance table containing mass-to-charge ratio, retention time, peak area information, compound names and molecular formulas. And (3) performing primary filtering on the data, reserving the characteristic substances with the Relative Standard Deviation (RSD) of less than 30% in the QC sample for further univariate and multivariate statistical analysis, and screening by taking the Variable Importance (VIP) value of more than 1 as a standard to obtain a difference variable, namely obtaining the characteristic compounds of the mutton samples with different storage times and different irradiation doses.
The normalization procedure was performed using auto-scaling and logarithmic transformation and the obtained data of the characteristic compounds of mutton samples at different storage times and irradiation doses were subjected to analysis of variance at the 0.05 probability level using the Metabioanalyst 4.0 analysis tool, followed by post-hoc examination of Fisher's LSD. Meanwhile, the clustering of the samples and the relative peak area change of the characteristic substances are visually displayed by utilizing the heat map. In addition, multivariate statistical analysis was performed on the data using SIMCA14.1, which mainly included unsupervised Principal Component Analysis (PCA) and supervised partial least squares-discriminant analysis (PLS-DA).
7. Analysis results
1) Effect of irradiation on Compound Change
A total of 103 key metabolites screened in positive ion mode (table 1) and negative ion mode (table 2) were responsible for metabolic changes between the five irradiated sample sets. Figure 2 is a heat map of the effect of the change in the content of differentially metabolized compounds in irradiated mutton in positive and negative modes. The right column in the figure lists the characteristic compounds corresponding to tables 1 and 2. The color difference of the 1.5 to-1.5 bands changes, indicating the change in the concentration of the compound in the sample. The rightmost Class band represents a different sample Class. The difference in color exhibited under the different treatment conditions in the graph indicates the magnitude of the change in the degree of the compound during ripening. As can be seen from FIG. 2, the levels of essential amino acids (such as L-phenylalanine, lysine, L-isoleucine and L-histidine) providing nutritive values in the mutton under irradiation treatment significantly changed, and the contents of L-phenylalanine and L-isoleucine in the irradiation group were higher, which was beneficial to meeting the human body requirements and maintaining nitrogen balance. Meanwhile, free amino acid and nucleotide are used as precursors influencing the flavor, and play an important role in the evaluation of the nutritional quality of the irradiated mutton.
2) Effect of storage time after irradiation treatment on Compound Change
Changes in compound content in mutton after irradiation treatment are shown in figure 3, and these related metabolites are significantly down-regulated during storage, except for L-phenylalanine and guanine. It is demonstrated that even if irradiation is an effective preservation method, long-term storage can cause the change of irradiated mutton metabolites, thereby causing the change of mutton quality. The results show that the irradiation dose and the storage time have obvious influence on the metabolites in the mutton.
3) Effect on metabolic pathways after irradiation treatment
And obtaining the total path of mutton metabolism under the influence of irradiation according to the content change of different endogenous metabolites of mutton and the path reported in the KEGG database. The result shows that the irradiation treatment obviously influences the change of mutton metabolites, and further influences the quality of mutton. Metabolic products of 5 irradiated samples were subjected to pathway enrichment analysis and topology analysis using metabolic analysis tool Metabioanalyser 4.0. The results show that 3 of the 20 predicted metabolic pathways are most significant, and the pathways mainly comprise the biosynthesis of phenylalanine, tyrosine and tryptophan, purine metabolism and phenylalanine metabolism, so that some understanding of mutton metabolite change in the irradiation process is clear.
Table 1 differential compounds in irradiated mutton identified in positive mode
Table 2 differential compounds in irradiated mutton identified in negative mode
The above-mentioned contents are only for illustrating the technical idea of the present invention, and the protection scope of the present invention is not limited thereby, and any modification made on the basis of the technical idea of the present invention falls within the protection scope of the claims of the present invention.
Claims (10)
1. A method for analyzing irradiated mutton metabonomics by ultra-high performance liquid chromatography-tandem mass spectrometry is characterized by comprising the following steps:
1) extracting an irradiated mutton sample to be detected by using an organic solvent, homogenizing, grinding, ultrasonically treating, incubating, centrifuging and drying to obtain a dried substance, and then re-dissolving, ultrasonically treating and centrifuging the dried substance to obtain a supernatant of the irradiated mutton sample to be detected;
2) determining the supernatant of the irradiated mutton sample to be detected by adopting a liquid chromatogram-tandem mass spectrometry, carrying out retention time comparison, peak extraction and integration, characteristic peak detection, background drift correction, database matching and data visualization processing on the obtained chromatographic data, carrying out unsupervised principal component analysis and supervised partial least square-discriminant analysis on the obtained result data set, screening to obtain a difference variable, obtaining a difference metabolite of the irradiated mutton sample to be detected, and establishing an integral metabolic spectrum of the irradiated mutton.
2. The method for analyzing the radiated mutton metabonomics by the ultra performance liquid chromatography-tandem mass spectrometry according to claim 1, wherein in the step 1, the organic solvent is a mixed solvent of methanol/acetonitrile/water with a volume ratio of 2:2: 1.
3. The method for analyzing the metabonomics of irradiated mutton by ultra performance liquid chromatography-tandem mass spectrometry according to claim 1, wherein in the step 1, the storage time of the irradiated mutton sample to be detected is 0, 30 and 60 days, and the irradiation dose is 0, 1, 2, 4 and 6 kGy.
4. The method for ultra performance liquid chromatography tandem mass spectrometry analysis of irradiated mutton metabonomics according to claim 1, wherein in step 1, the grinding time is 5min, the vortex is 30s, the ultrasound time is 10min, the incubation is performed at-20 ℃ for 20min, the centrifugation is performed at 10000 Xg for 20min, and the centrifugation is performed at 10000 Xg for 15min after the redissolution.
5. The method for analyzing the metabonomics of irradiated mutton by ultra performance liquid chromatography-tandem mass spectrometry according to claim 1, wherein in the step 2, the supernatant of the irradiated mutton sample to be detected is filtered by a 0.22 μm membrane before being measured.
6. The method for ultra performance liquid chromatography tandem mass spectrometry analysis of irradiated mutton metabonomics according to claim 1, wherein in the step 2, the liquid chromatography conditions are as follows: hypersil Gold chromatographic column, mobile phase A is 0.1% formic acid in water, mobile phase B is 0.1% formic acid in acetonitrile; the column temperature is 30 ℃, the sample injection volume is 5 mu L, and the flow rate is 0.3 mL/min; the elution gradient was: 0-1 min, 15% B, 1-7 min, 15-40% B, 7-9 min, 40-70% B, 9-14 min, 70-100% B, 14-17 min, 100% B, 17-17.1 min, 100-15% B, 17.1-20 min, 15% B.
7. The method for ultra performance liquid chromatography tandem mass spectrometry analysis of irradiated mutton metabonomics according to claim 1, wherein in step 2, the mass spectrometry conditions are as follows: the ion source is an electrospray ionization source and adopts a positive ion and negative ion detection mode; the flow rate of the sheath gas is 48-50 arb; the flow rate of the auxiliary gas is 11-15 arb; the temperature of the capillary tube is 300-350 ℃; the heating temperature of the auxiliary gas is 360-400 ℃.
8. The method for ultra performance liquid chromatography tandem mass spectrometry analysis of irradiated mutton metabonomics according to claim 1, wherein the mass spectrometry adopts Full MS and dd-MS 2 In the scanning mode, in the Full MS scanning mode, the scanning range m/z is 100-1500, the Full MS scanning resolution is 70000FWHM, and the AGC is 1e 6 (ii) a At dd-MS 2 In scan mode, the mass resolution is 17500FWHM, AGCIs 2e 5 。
9. The method for analyzing the radiated mutton metabonomics by the ultra performance liquid chromatography-tandem mass spectrometry according to claim 1, wherein the difference variables are obtained by screening with the variable importance value larger than 1 as a standard, so as to obtain the difference metabolites of the mutton sample to be detected.
10. The method for analyzing irradiated mutton metabonomics according to claim 1, wherein the variance analysis on the 0.05 probability level is performed on the obtained differential metabolite data.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108333270A (en) * | 2018-02-08 | 2018-07-27 | 中央民族大学 | A kind of analysis method of Artemisia rupestris Plant Metabolome |
| CN111413436A (en) * | 2020-04-23 | 2020-07-14 | 中国农业科学院农业质量标准与检测技术研究所 | Method for identifying lamb mutton and adult mutton |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108333270A (en) * | 2018-02-08 | 2018-07-27 | 中央民族大学 | A kind of analysis method of Artemisia rupestris Plant Metabolome |
| CN111413436A (en) * | 2020-04-23 | 2020-07-14 | 中国农业科学院农业质量标准与检测技术研究所 | Method for identifying lamb mutton and adult mutton |
Non-Patent Citations (1)
| Title |
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| 刘春泉等: "辐照处理对冷冻羊肉品质的影响", 《核农学报》, vol. 28, no. 6, pages 1018 * |
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