CN114957429A - Broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs 60-81 And uses thereof - Google Patents
Broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs 60-81 And uses thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43509—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from crustaceans
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs 60‑81 And the application thereof, the molecular formula of which is C 109 H 192 N 42 O 26 S 1 The amino acid sequence is shown in SEQ ID NO. 01. The antibacterial peptide has broad-spectrum antibacterial activity, strong antibacterial effect, high sterilization efficiency, no cytotoxicity and high safety, can be used as an effective component and applied to antibacterial compositions, antifungal compositions and aquatic feedsAnd has wide application prospect.
Description
Technical Field
The invention belongs to the technical field of marine molecular biology, and particularly relates to a scyrephein broad-spectrum antibacterial polypeptide of blue crabs 60-81 And applications thereof.
Background
The overuse of antibiotics leads to the continuous enhancement of the drug resistance of microorganisms, and brings serious threat to human health. The problem of antibiotic resistance is a major scientific and technological problem which affects the sustainable development of livestock husbandry and aquaculture industries and human health at present.
Antibacterial Peptides (antibiotics Peptides) are a class of micromolecular polypeptides widely existing in nature, are important components of the innate immune system of organisms, play an important role in defending pathogenic microorganisms, and are praised as 'natural antibiotics' by scientists. A variety of antimicrobial peptides have been found to have antibacterial, antifungal, viral, parasitic, etc. activity, as well as biological functions such as antitumor, neutralizing toxins, immunomodulating, and wound repair. Compared with the traditional antibiotics, the antibacterial peptide has the advantages of heat stability, difficult generation of drug resistance, no harmful residue and the like, so the antibacterial peptide is taken as an effective substitute of the antibiotics and has important potential application value in a plurality of fields such as medicine, livestock raising and aquaculture industry, food and the like. Since the 80 s of the 20 th century, Boman et al discovered cecropins (hyatophora cecropia) with alpha-helix structure from the ancient ratio silkworm (hyatophora cecropin), and then discovered and isolated several antibacterial peptides in succession from bacteria, fungi, amphibians, mammals, plants, etc., and now three thousand more antibacterial peptides were collected in the APD database 3 (https:// aps. unmac. edu /).
The sea has about 80% of biological resources on earth, the number of marine invertebrates is large, blue crabs belong to the invertebrates, and the blue crabs mainly rely on various innate immune systems to resist the attack of pathogenic microorganisms or harmful substances in the marine environment. The antibacterial peptides found in the Scylla paramamosain comprise anti-lipopolysaccharide factors (ALFs), chitin (crustin), SpHyastatin, SpPR-AMP1, arasin-like Sp and GRPSp, scygonadin, sphistin, scyreplacin and the like, in recent years, scholars at home and abroad highly realize that abundant antibacterial active substances exist in marine organisms, and the discovery and utilization of the antibacterial peptides derived from the marine organisms play irreplaceable important roles in the aspects of future breeding industry, food, medicine and the like and have great development and utilization values.
Disclosure of Invention
The invention aims to provide a broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs 60-81 。
Another objective of the invention is to provide the Scyrephemin broad-spectrum antibacterial polypeptide of blue crab 60-81 The use of (1).
The technical scheme of the invention is as follows:
broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs 60-81 The molecular formula is C 109 H 192 N 42 O 26 S 1 The amino acid sequence is shown in SEQ ID NO. 01.
The broad-spectrum antibacterial polypeptide Scyrephemin of the blue crab 60-81 Use in the preparation of an antibacterial composition.
In a preferred embodiment of the invention, it has an inhibiting and killing effect on Staphylococcus aureus, Corynebacterium glutamicum, Micrococcus muralyticus, enterococcus faecium, enterococcus faecalis, Escherichia coli, Acinetobacter baumannii, Shigella flexneri, Pseudomonas aeruginosa, Pseudomonas stutzeri, Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio fluvialis and Vibrio harveyi.
An antifungal composition contains Scyrephemin as effective component 60-81 。
The broad-spectrum antibacterial polypeptide Scyrephemin of the blue crab 60-81 Application in the preparation of antimycotic compositions.
In a preferred embodiment of the invention, it has inhibitory and killing effects on Aspergillus niger, Aspergillus fumigatus and Aspergillus ochraceus.
An aquatic feed additive contains the broad-spectrum antibacterial polypeptide Scyrephemin as effective component 60-81 。
The broad-spectrum antibacterial polypeptide Scyrephemin of the blue crab 60-81 Application in preparing aquatic feed additive.
In a preferred embodiment of the present invention, it has an inhibiting and killing effect on Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio fluvialis and Vibrio harveyi.
The invention has the beneficial effects that:
1. the invention consists of 22 amino acids, and the molecular formula is C 109 H 192 N 42 O 26 S 1 The molecular weight is 2539.05 daltons, HeliQuest predicts the charge number of the antibacterial peptide to be +6, predicts the isoelectric point to be 12.30 and predicts the average coefficient of hydrophilicity to be-0.123, and the antibacterial peptide is a cationic polypeptide with positive charges.
2. The invention has obvious antibacterial effect on bacteria and mould, and simultaneously has no cytotoxicity on normal mammal cells, human kidney epithelial cells and scylla paramamosain blood cells under high concentration.
3. The invention has broad-spectrum antibacterial activity, strong antibacterial effect, quick sterilization efficiency and good antibacterial effect on common aquatic pathogenic bacteria; the compound is derived from crustacean, has no cytotoxicity and high safety, can be used as an effective component, is applied to antibacterial compositions, antifungal compositions and aquatic feeds, and has wide application prospect.
Drawings
FIG. 1 shows a scy broad-spectrum antibacterial polypeptide Scyrephemin of blue crab in example 3 of the present invention 60-81 Graph of bactericidal kinetics for staphylococcus aureus and acinetobacter baumannii with time (min) on the abscissa and bactericidal index (%) on the ordinate.
FIG. 2 shows the scyrphemin as a broad-spectrum antimicrobial polypeptide of blue crab in example 4 of the present invention 60-81 Thermal stability plots of antibacterial activity against Staphylococcus aureus and Acinetobacter baumannii, where the abscissa is time (h) and the ordinate is OD 600 The value is obtained.
FIG. 3 shows the scyrphemin as the broad-spectrum antimicrobial polypeptide of blue crab in example 5 of the present invention 60-81 Scanning electron microscope observation images of treated staphylococcus aureus and acinetobacter baumannii. Wherein A is Staphylococcus aureus, and B is goldStaphylococcus aureus + 48. mu. MScyrephein 60-81 C is Acinetobacter baumannii, D is Acinetobacter baumannii +48 mu M Scyrephemin 60-81 。
FIG. 4 shows the scyrphemin as the broad-spectrum antimicrobial polypeptide of blue crab in example 6 of the present invention 60-81 Experimental diagram for inhibiting spore germination of Aspergillus niger, Aspergillus fumigatus and Aspergillus ochraceus (from left to right), wherein Scyrephemin 60-81 The final concentrations from top to bottom were: 0. mu.M, 3. mu.M, 6. mu.M, 12. mu.M and 24. mu.M.
FIG. 5 shows that the MTS-PMS method for detecting the Scyrephemin, a broad-spectrum antibacterial polypeptide of blue crab in example 7 of the invention 60-81 Cytotoxicity assay graphs of (a); wherein the abscissa represents antibacterial polypeptide Scyrephemin 60-81 The concentration (. mu.M) of (C) in the ordinate represents the cell growth rate (%).
Detailed Description
The technical solution of the present invention will be further illustrated and described below with reference to the accompanying drawings by means of specific embodiments.
Example 1 Scyrephemin, a broad-spectrum antimicrobial polypeptide of blue crab 60-81 Preparation of
The broad-spectrum antibacterial polypeptide Scyrephemin of the blue crab 60-81 The amino acid sequence of (a) is:
Arg-Cys-Ile-Leu-Gly-Ala-His-Phe-Arg-Thr-Leu-Arg-Ala-Thr-Arg-Thr-Ala-Gly-Leu-Arg-Arg-Leu(SEQ ID NO.01)
the Scyrephemin antibacterial polypeptide with the purity of more than 95 percent can be obtained by adopting the existing solid-phase chemical synthesis method 60-81 . The Scyrephemin serving as the antibacterial polypeptide of Scyrephemin 60-81 The molecular weight and HPLC detection information of the polypeptide are obtained and provided by entrusting Kingsry Biotechnology Co., Ltd through solid phase synthesis. Scyrephemin 60-81 The physicochemical parameters are shown in table 1:
TABLE 1 Scyrephemin 60-81 Physical and chemical parameters of
From Table 1, Scyrephemin is known 60-81 Small molecular weight and stabilityThe polypeptide is preferably a cationic polypeptide with positive charges.
Example 2 antimicrobial polypeptide Scyrephemin 60-81 Determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)
The strains involved in this example were: staphylococcus aureus (Staphylococcus aureus), Micrococcus lysodeikticus (Micrococcus lysodeikticus), Enterococcus faecium (Enterococcus faecium), Enterococcus faecalis (Enterococcus faecium), Corynebacterium glutamicum (Corynebacterium glutamicum), Escherichia coli (Escherichia coli), Shigella flexneri (Shigella flexneri), Acinetobacter baumannii (Acinetobacter baumannii), Pseudomonas Aeruginosa (Pseudomonas Aeruginosa), Pseudomonas stutzeri (Pseudomonas stutzeri), Aeromonas hydrophila (Aeromonas hydrophila), Pseudomonas fluorescens (Pseudomonas fluorescens), Vibrio fluvialis), Vibrio harveyi (Vibrio harveyi), Vibrio bacteriolyticus (Vibrio bacteriolyticus), Aspergillus niger (Aspergillus niger) and Aspergillus niger (Aspergillus niger). The strains are purchased from the strain preservation center of the institute of microbiology, China academy of sciences, and are preserved and stored in the laboratory.
The specific method comprises the following steps:
(1) coating the preserved staphylococcus aureus, micrococcus muralis, enterococcus faecium, enterococcus faecalis, corynebacterium glutamicum, escherichia coli, shigella flexneri, acinetobacter baumannii, pseudomonas aeruginosa, pseudomonas stutzeri, aeromonas hydrophila and pseudomonas fluorescens on a nutrient broth plate, and performing inverted culture at each appropriate temperature for 18-24 h; spreading Vibrio fluvialis, Vibrio harveyi and Vibrio alginolyticus on 2216 plate, and inversely culturing at 28 deg.C for 18-24 h; aspergillus niger, Aspergillus fumigatus and Aspergillus ochraceus were spread on potato dextrose plates and cultured in an inverted culture at 28 ℃ for 1-7 days.
(2) Colonies from each plate were picked and inoculated on the corresponding media slant and the bacteria were cultured for a further 18-24 h. Bacteria were washed off the slant with 10mM sodium phosphate buffer (pH 7.4) to adjust the concentration of the bacterial suspension. Dilution of bacteria with MH liquid MediumThe final concentration of the cells was 5X 10 by diluting the Vibrio with a seawater medium 5 CFU/mL. The fungal spores were washed off the slant with 10mM sodium phosphate buffer (pH 7.4), diluted with potato dextrose broth and sodium phosphate buffer mixture, counted under an optical microscope using a hemocytometer, and the spore concentration was adjusted so that the final fungal spore concentration was 5 × 10 4 one/mL.
(3) Synthesized Scyrephemin 60-81 The powders were dissolved in sterile MilliQ water, filtered through 0.22. mu.M filter, and then diluted to 3. mu.M, 6. mu.M, 12. mu.M, 24. mu.M, 48. mu.M, and 96. mu.M protein concentrations by fold, and placed on ice for use.
(4) On a 96-well cell culture plate, each bacterium to be tested is provided with a blank control group, a negative control group and an experimental group to be tested, and each group is provided with three parallels:
a blank control group: 50 mu L of protein sample to be detected and 50 mu L of culture medium;
b negative control group: 50 μ L of sterile MilliQ water and 50 μ L of bacterial suspension;
c test group: 50 μ L of the protein sample to be tested and 50 μ L of the bacterial suspension.
(5) Placing the 96-hole cell culture plate in an incubator at 28 ℃, culturing for 1-2d, and observing the MIC result in the experimental group to be tested; and blowing and uniformly mixing the experimental group to be detected, sucking a proper amount of bacterial liquid, dripping the bacterial liquid on a corresponding solid culture medium flat plate, carrying out inverted culture for 1-2 days at a proper temperature, and observing an MBC result.
Scyrephemin as an antibacterial polypeptide of Scyrephemin 60-81 Has broad-spectrum antibacterial activity, and has strong antibacterial and bactericidal effects on Micrococcus muralis, Corynebacterium glutamicum, enterococcus faecium, Shigella flexneri and Pseudomonas stutzeri, and the minimum bactericidal concentration is less than 12 μ M; the bactericidal composition has good inhibition and killing effects on staphylococcus aureus, escherichia coli, acinetobacter baumannii, pseudomonas aeruginosa, pseudomonas fluorescens, aspergillus niger, aspergillus fumigatus and aspergillus ochraceus, and the minimum bactericidal concentration is 12-24 mu M; has certain inhibiting and killing effects on enterococcus faecalis, aeromonas hydrophila, vibrio fluvialis and vibrio harveyi, and the minimum bactericidal concentration is 24-48 mu M. The results are shown in table 2:
TABLE 2 antimicrobial polypeptide Scyrephemin 60-81 Antibacterial activity of
Note: MIC: the minimum inhibitory concentration (. mu.M), indicated as a-b. a: the highest protein concentration of the thallus growth can be seen by naked eyes; b: the lowest protein concentration for the growth of the cells was not seen visually. MBC: minimum bactericidal concentration (. mu.M), indicated as a-b. a: the highest protein concentration for the visible colony growth on the plate; b: the lowest protein concentration at which colonies grew was not seen on the plates.
Example 3 antimicrobial polypeptide Scyrephemin 60-81 Kinetics curve of sterilization
In the embodiment, staphylococcus aureus and acinetobacter baumannii are selected as bacteria to be detected, and the broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs is obtained 60-81 The bactericidal kinetics of (a) were determined.
The specific procedure was similar to the antimicrobial activity assay described in example 2. Adjustment of Scyrephemin 60-81 To a final concentration of 1-fold MBC (Staphylococcus aureus: 24. mu.M; Acinetobacter baumannii: 24. mu.M), Scyrephemin 60-81 After incubating with the tested bacteria for a certain time, taking 6 mu L of bacterial suspension for dilution, taking a proper amount of the diluted bacterial suspension to coat on a nutrient broth plate, and culturing at 37 ℃ for 1-2d for clone counting. The samples incubated with sterile MilliQ water and bacterial suspension for 0h were used as positive control, 6. mu.L of clones plated on nutrient broth plates at the same dilution was defined as 100%, and the bactericidal index was expressed as the percentage of the number of clones in the experimental group incubated for a certain period of time relative to the number of clones in the positive control, as shown in FIG. 1, Scyrephemin 60-81 Has rapid bactericidal effect, and the bactericidal index of staphylococcus aureus and acinetobacter baumannii reaches 100% within 10 min.
Example 4 antimicrobial polypeptide Scyrephemin 60-81 Antimicrobial active thermal stability
In the embodiment, staphylococcus aureus and acinetobacter baumannii are selected as bacteria to be detected, and the broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs is obtained 60-81 The heat stability of the antibacterial activity was measured.
The specific procedure was similar to the antimicrobial activity assay described in example 2. Adjustment of Scyrephemin 60-81 To a final concentration of 1-fold MBC (Staphylococcus aureus: 24. mu.M; Acinetobacter schbaumannii: 24. mu.M), the antimicrobial polypeptide Scyrephemin was added 60-81 Heating in 100 deg.C boiling water for 10min, 20min, 30min, and placing on ice for use. Mixing Scyrephemin 60-81 Incubating with bacteria to be tested, and measuring OD with enzyme labeling instrument at 0h, 12h, 24h, 36h, and 48h 600 Value of (c), Scyrephemin as shown in FIG. 2 60-81 After 30min of boiling water bath, the antibacterial activity is still kept.
Example 5 SEM Observation of Scyrephemin 60-81 Morphological structural change of bacteria after treatment
In the embodiment, staphylococcus aureus and acinetobacter baumannii are selected as strains to be detected, and the preparation of the scanning electron microscope sample is carried out according to the following steps:
(1) activating strains, randomly selecting 3-5 clones to corresponding nutrient broth liquid culture medium when the clones grow to a proper size, shaking to logarithmic growth phase, measuring OD, centrifuging to remove supernatant, resuspending the strains by using MH culture medium, adjusting OD to 0.1, and placing on ice for later use.
(2) The polypeptide powder was solubilized using sterile MilliQ and filtered through a 0.22 μ M filter to adjust the final polypeptide concentration to 2-fold MBC. Respectively setting an experimental group and a control group, uniformly mixing, and then incubating for 1h at 37 ℃. The supernatant was centrifuged off, and the cells were collected after washing once with PBS.
(2) The collected cells were resuspended with 2.5% glutaraldehyde and fixed in a freezer at 4 ℃ for 1.5h or more. After the fixed thalli is washed for three times by PBS, high-concentration suspension is prepared, the suspension is dripped on a cut glass slide, the adhesion is carried out for 30min, and ethanol gradient dehydration is carried out after the filtration is carried out by using filter paper.
(3) 30% -50% -70% -80% -90% -95% -100% (v/v) ethanol is dehydrated step by step, and each step of dehydration lasts for 10-15 min.
(4) Drying the critical point, then spraying gold for 60s at 10mA current; observing by a scanning electron microscope and shooting and recording.
The results are shown in FIG. 3, where the control group had normal morphology, intact structure, smooth surface and no shrinkage. Antimicrobial polypeptide Scyrephemin 60-81 The treated bacteria are gathered, collapse and fold appear on the surface, the thalli are broken, and substances in the cells leak out.
Example 6 antimicrobial polypeptide Scyrephemin 60-81 Optical microscope observation of post-action fungal spore germination
In the embodiment, aspergillus fumigatus, aspergillus ochraceus and aspergillus niger are selected as bacteria to be detected, and the broad-spectrum antibacterial polypeptide Scyrephemin of the blue crabs is observed 60-81 Influence on the germination of fungal spores.
The specific procedure was similar to the antimicrobial activity assay described in example 2. Adjustment of Scyrephemin 60-81 The concentration is 6 μ M, 12 μ M, 24 μ M and 48 μ M, and the mixture is placed on ice for standby; adjusting the final concentration of each fungal spore to 5 × 10 4 one/mL. Equal volume of each concentration of Scyrephemin 60-81 Mixing with each fungal spore in 96-well cell culture plate, setting blank control group, placing in 28 deg.C incubator, standing for 24 hr, and observing fungal spore germination under optical microscope. Scyrephemin as shown in FIG. 4 60-81 At a concentration of 12 μ M, germination of spores of Aspergillus fumigatus and Aspergillus ochraceus was inhibited, and at a concentration of 24 μ M, Aspergillus niger, Aspergillus fumigatus and Aspergillus ochraceus were completely killed.
Example 7 antimicrobial polypeptide Scyrephemin 60-81 Cytotoxicity assays
In the embodiment, human kidney epithelial cells (HEK-293T) and normal blood cells of Scyrephemin, which is a broad-spectrum antibacterial polypeptide for blue crabs, are selected 60-81 Cytotoxicity was measured.
(1) Collecting Eriocheir sinensis blood cells and human renal epithelial cells with good growth state, counting with cell counting plate, and adjusting cell concentration to 1 × 10 with culture medium 5 Mixing the cell suspension uniformly, adding 100 mu L of cell suspension into each hole of a 96-hole cell culture plate, and placing the cell suspension in an incubator at a proper temperature to culture more than 80% of cells attached to the wall.
(2) Carefully aspirate the medium, add 100 μ L of the antimicrobial polypeptide diluted with medium gradient to concentrations of 3, 6, 12, 24, 48, 96 μ M, and incubate at the appropriate temperature for 24 h.
(3) Adding 20 mu LMTS-PMS solution into each well, incubating for 2h in dark place, and measuring OD with microplate reader 492 Value, evaluation of Scyrephemin 60-81 The cytotoxicity of (a).
The results are shown in FIG. 5, Scyrephemin concentrations as high as 96. mu.M 60-81 Has no cytotoxicity to scylla paramamosain blood cells and human kidney epithelial cells.
The above description is only a preferred embodiment of the present invention, and therefore should not be taken as limiting the scope of the invention, which is defined by the appended claims.
Sequence listing
<110> university of mansion
Xiamen Jiacheng Biotechnology Co.,Ltd.
<120> broad-spectrum antimicrobial polypeptide Scyrephemin 60-81 of blue crab and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 22
<212> PRT
<213> Scylla Paramosain)
<400> 1
Ala Cys Ile Leu Gly Ala His Pro Ala Thr Leu Ala Ala Thr Ala Thr
1 5 10 15
Ala Gly Leu Ala Ala Leu
20
Claims (10)
1. Broad-spectrum antimicrobial polypeptide Scyrephemin for blue crabs 60-81 The method is characterized in that: the molecular formula is C 109 H 192 N 42 O 26 S 1 The amino acid sequence is shown as SEQ ID NO. 01.
2. RightsThe broad-spectrum antimicrobial polypeptide Scyrephemin of claim 1 60-81 Use in the preparation of an antibacterial composition.
3. An antibacterial composition characterized by: the effective components of the scylla serrata antibacterial polypeptide Scyrephein comprise the scylla serrata antibacterial polypeptide of claim 1 60-81 。
4. An antibacterial composition according to claim 3, wherein: it has inhibiting and killing effects on Staphylococcus aureus, Corynebacterium glutamicum, Micrococcus muralyticus, enterococcus faecium, enterococcus faecalis, Escherichia coli, Acinetobacter baumannii, Shigella flexneri, Pseudomonas aeruginosa, Pseudomonas stutzeri, Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio fluvialis and Vibrio harveyi.
5. The scylla serrata broad-spectrum antibacterial polypeptide Scyrephein of claim 1 60-81 Use in the preparation of an anti-mould composition.
6. An antifungal composition, comprising: the effective components of the scylla serrata antibacterial polypeptide Scyrephein comprise the scylla serrata antibacterial polypeptide of claim 1 60-81 。
7. An anti-mold composition according to claim 6, wherein: it has inhibiting and killing effects on Aspergillus niger, Aspergillus fumigatus and Aspergillus ochraceus.
8. The scylla serrata broad-spectrum antibacterial polypeptide Scyrephein of claim 1 60-81 Application in preparing aquatic feed additive.
9. An aquatic feed additive, which is characterized in that: the effective components of the scylla serrata antibacterial polypeptide Scyrephein comprise the scylla serrata antibacterial polypeptide of claim 1 60-81 。
10. An aquaculture feed additive according to claim 8 wherein: it has inhibiting and killing effects on Aeromonas hydrophila, Pseudomonas fluorescens, Vibrio fluvialis and Vibrio harveyi.
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| CN110028568A (en) * | 2019-03-11 | 2019-07-19 | 厦门大学 | A kind of Scylla paramamosain antibacterial polypeptide Sp-NPFin and its application |
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