CN114957026A - Method for extracting natural betaine from yeast wastewater - Google Patents
Method for extracting natural betaine from yeast wastewater Download PDFInfo
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- CN114957026A CN114957026A CN202210619990.1A CN202210619990A CN114957026A CN 114957026 A CN114957026 A CN 114957026A CN 202210619990 A CN202210619990 A CN 202210619990A CN 114957026 A CN114957026 A CN 114957026A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
- C07C227/42—Crystallisation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/02—Treatment of water, waste water, or sewage by heating
- C02F1/04—Treatment of water, waste water, or sewage by heating by distillation or evaporation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/22—Treatment of water, waste water, or sewage by freezing
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/38—Treatment of water, waste water, or sewage by centrifugal separation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/42—Treatment of water, waste water, or sewage by ion-exchange
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/444—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by ultrafiltration or microfiltration
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/42—Treatment of water, waste water, or sewage by ion-exchange
- C02F2001/425—Treatment of water, waste water, or sewage by ion-exchange using cation exchangers
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
A method for extracting natural betaine from yeast wastewater comprises the following steps: step 1: carrying out impurity removal pretreatment on the yeast wastewater; step 2: introducing the pretreated liquid obtained in the step 2 into an ultrafiltration membrane system, and carrying out cross-flow filtration under the pressure of 1-5 kg to obtain a concentrated solution which is concentrated by 3-10 times; and step 3: introducing the filtrate obtained in the step 2 into a continuous chromatography system to obtain an extract component rich in betaine and a raffinate component rich in salt and other impurities; and 4, step 4: decoloring and concentrating the extracting solution rich in betaine to obtain a betaine liquid product with the mass fraction of 50-85%; or cooling and crystallizing or evaporating the betaine-rich extract to obtain high-purity natural betaine crystal product with purity of 95-99.9%. After the pretreatment process, the invention adopts the continuous chromatography technology to separate the betaine, does not need to add any chemical in the process, belongs to an efficient physical separation process and has low operation cost.
Description
Technical Field
The invention relates to a method for extracting natural betaine from yeast wastewater, belonging to the technical field of wastewater recovery treatment.
Background
At present, yeast is widely applied to food processing, wine brewing,The food is closely related to human life and cannot be separated. The yeast is produced by mainly using molasses as a raw material, wherein cane molasses, beet molasses and starch sugar are mainly used. The molasses has complex components and contains a large amount of inorganic salts and macromolecular organic saccharides. The sugar cane and the beet are easy to form furfural in the sugar heating process, further to form dark caramel compounds, the caramel compounds exist in the yeast wastewater to darken the color of the yeast wastewater, and meanwhile, macromolecular organic matters and inorganic salts in the wastewater, especially K + and SO 4 2- The concentration of (A) is higher, which all increase the difficulty of treating the yeast wastewater.
The waste water produced in the yeast production process is mainly produced in the processes of molasses pretreatment, yeast fermentation liquor separation and yeast vacuum filtration, the COD content produced by the waste water accounts for more than 90% of the total COD discharged in the yeast production process, and then the waste water produced in the yeast production process is cleaned. The pollutants discharged by yeast production mainly come from molasses per se, the yeast fermentation production utilizes sugar substances which can be utilized by fermentation in the molasses, and substances which are not absorbed and utilized by the yeast and metabolites produced by metabolism of the yeast are finally discharged with waste water. Therefore, the yeast fermentation waste liquid contains abundant inorganic trace elements such as nitrogen, phosphorus, potassium, calcium, iron and the like, and organic substances such as humic acid, fulvic acid and the like.
The yeast wastewater treatment method comprises the following steps:
(1) and the publication No. CN 104787823 discloses a yeast wastewater zero discharge treatment method, the yeast wastewater is further concentrated and dried, and then spray-dried to prepare the organic compound fertilizer.
(2) CN102874987 discloses a pretreatment method of yeast wastewater, which comprises the steps of adjusting pH value of raw water of yeast wastewater to be acidic, then carrying out aeration treatment, finally adjusting pH value to 7-8, and then entering a conventional biological treatment system.
(3) CN102776239B discloses a microbial flocculant produced by using yeast wastewater and beer wastewater and a method thereof, the yeast wastewater and the beer wastewater are mixed, phosphate is added to obtain a mixed wastewater culture medium, the mixed wastewater culture medium is inoculated with aspergillus fumigatus original variety, and the microbial flocculant is produced by fermentation
(4) The yeast wastewater treatment method disclosed by the publication No. CN101734827A comprises the steps of firstly carrying out coagulating sedimentation pretreatment on wastewater, then carrying out anaerobic treatment on the pretreated wastewater, then carrying out aerobic treatment on the wastewater after the anaerobic treatment, and finally carrying out deep oxidation treatment on the wastewater after the aerobic treatment by adopting a Fenton advanced oxidation method and air flotation.
(5) The publication No. CN102060341B discloses a yeast wastewater treatment method, and a feed additive and a feed product obtained by the method, and provides a yeast high-concentration organic wastewater treatment method, a feed additive obtained by the method, and a feed product containing the feed additive. The yeast high-concentration organic wastewater can be treated to be simultaneously changed into fertilizer and feed additive for utilization, so that the economic benefit of the yeast wastewater is improved, and the cost for treating the yeast wastewater is greatly reduced, thereby achieving the optimal comprehensive utilization of resources; in addition, the content of potassium in the fertilizer can be increased, so that high-grade potassium salt fertilizer can be obtained.
(5) The process for extracting betaine from the beet molasses fermentation waste liquid disclosed in the publication No. CN10493333 comprises the processes of raw material pretreatment, ion exchange, concentration crystallization and refining, and has the characteristics of short flow, less auxiliary materials, low cost, no pollution and suitability for industrialization because the processes of active carbon decolorization and anion resin exchange are omitted and ammonium bicarbonate is used as an eluent instead of ammonium hydroxide before cation exchange.
The traditional method for treating the yeast wastewater basically treats the yeast wastewater as a tail end, or produces fertilizer or feed by evaporating and concentrating all components of the yeast wastewater, and does not fully utilize valuable resources in the yeast wastewater; in the yeast wastewater produced based on the beet molasses, sugar is fully utilized to produce yeast, but betaine, salt and other impurities cannot be fully utilized by the yeast, so that the yeast wastewater is enriched; the betaine content of the yeast wastewater is 10-15% of the dry basis. The traditional yeast wastewater treatment process is undoubtedly a waste of resources for the natural betaine contained therein.
CN10493333 extracts betaine from beet molasses fermentation waste liquid, the process core is to exchange the betaine to an ion exchange column by means of ion exchange, and then replace the betaine by chemicals and regenerate the betaine; when the method is used for adsorbing and exchanging the betaine, other ions are exchanged in a considerable amount, and a large amount of salt is eluted while the betaine is eluted, so that the purity of the betaine is low; because the purity of the betaine produced by the method is low, only equivalent hydrochloric acid can be additionally used for producing betaine hydrochloride products, but natural betaine products cannot be produced; the method has high chemical consumption, and the obtained betaine exists in the form of hydrochloride, and is mainly applied to low-end feed markets, but not applied to high-end markets such as food additives, cosmetics or medicine fields.
Disclosure of Invention
The invention aims to provide a method for extracting natural betaine from yeast wastewater.
In order to achieve the above objects and other related objects, the present invention provides the following technical solutions: a method for extracting natural betaine from yeast wastewater comprises the following steps:
step 1: carrying out impurity removal pretreatment on the yeast wastewater;
step 2: introducing the pretreated liquid obtained in the step 2 into an ultrafiltration membrane system, and performing cross-flow filtration under the pressure of 1-5 kg to obtain a filtrate;
and step 3: introducing the filtrate obtained in the step 2 into a continuous chromatography system to obtain an extract component rich in betaine and a raffinate component rich in salt and other impurities;
and 4, step 4: decoloring and concentrating the extracting solution rich in betaine to obtain a betaine liquid product with the mass fraction of 50-85%; or cooling and crystallizing or evaporating the betaine-rich extract to obtain high-purity natural betaine crystal product with purity of 95-99.9%.
The preferable technical scheme is as follows: the impurity removal pretreatment comprises at least one of centrifugal separation, chemical flocculation or chemical precipitation reaction.
The preferable technical scheme is as follows: the ultrafiltration membrane system adopts a ceramic membrane or an organic membrane, the filtration precision is 1000 daltons-50 ten thousand daltons, and the filtration temperature is 30-85 ℃.
The preferable technical scheme is as follows: the continuous chromatography system consists of 4-10 chromatographic columns.
The preferable technical scheme is as follows: the chromatographic column is filled with cation exchange resin which is in a sodium type, a potassium type, a calcium type or a hydrogen type; the particle size of the cation exchange resin is 200-500 microns.
The preferable technical scheme is as follows: in the step 3, the mass concentration of the feeding material during chromatography is 20-60%, the working temperature is 40-80 ℃, and the feeding flow rate is 1-10 m/h.
Due to the application of the technical scheme, compared with the prior art, the invention has the advantages that:
1. the invention uses the yeast factory production wastewater containing betaine as raw material to extract natural betaine, thereby changing waste into valuable.
2. The invention adopts physical or chemical pretreatment, effectively prevents protein and polysaccharide colloids from polluting the downstream process, and ensures that the downstream purification process can be continuously carried out.
3. The advanced pretreatment of the invention adopts an ultrafiltration membrane system, thereby further ensuring that the resin of the chromatography system is not polluted.
4. The invention adopts continuous chromatography, does not need to add any chemical in the process of separating the betaine, belongs to a high-efficiency physical separation process and has low operation cost.
5. The crystallization mother liquor of the invention is circulated to the chromatography feeding, further improving the product yield.
Drawings
FIG. 1 is a flow chart of the process of the present invention.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will be apparent to those skilled in the art from the present disclosure.
Please refer to fig. 1. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only for the purpose of understanding and reading the disclosure, and are not to be construed as limiting the scope of the present invention, which is defined by the claims and the appended claims. The following examples are provided for a better understanding of the present invention, and are not intended to limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The experimental materials used in the following examples were all purchased from a conventional biochemical reagent store unless otherwise specified.
Example 1: method for extracting natural betaine from yeast wastewater
Collecting fermented liquor containing betaine, i.e. concentrated wastewater after yeast production, and performing impurity removal pretreatment by filtering to remove impurities, centrifuging to remove impurities or adding flocculant into the wastewater, standing for precipitation, and filtering for separation. Or after adding the flocculating agent, removing impurities by a centrifugal mode. Obtaining the pretreated liquid through centrifugal treatment, chemical flocculation treatment or precipitation reaction treatment and the combination of the above treatment processes.
And carrying out advanced treatment after pretreatment, introducing the pretreated crude product into a molecular-level ultrafiltration membrane system (a ceramic membrane or an organic membrane, preferably a ceramic membrane), carrying out ultrafiltration with the filtration precision of 1000-50 ten thousand daltons, carrying out cross-flow filtration at the filtration temperature of 30-85 ℃ under the pressure of 1-5 kg, concentrating by 3-10 times, then carrying out dialysis treatment on the filtrate, and further recovering the product.
Removing impurities from a material by using an ultrafiltration membrane, introducing the material into a continuous chromatography system, wherein the continuous chromatography system consists of 4-10 columns, a simulated moving bed or an improved simulated moving or sequential simulated moving working mode can be adopted, cation exchange chromatography resin is filled in the chromatography columns, typical resin ion forms include but are not limited to a sodium type, a potassium type, a calcium type or a hydrogen type, a preferred type is a sodium type or a potassium type, the particle size of the resin is 200-500 micrometers, the feeding concentration is 20-60%, the temperature is 40-80 ℃, and the average feeding speed is 1-10 m/h; the eluent is deionized water or evaporated condensed water. After continuous chromatography, the feed is separated into two different materials, namely an extract component (with the content of 50-85%) rich in betaine and a raffinate component rich in salt and other impurities.
Decolorizing the betaine-rich component with activated carbon or resin or nanofiltration membrane, selecting powdered carbon or granular carbon as the activated carbon according to local resources, and concentrating to obtain 50-85% purity betaine liquid product; or cooling crystallization or evaporative crystallization is carried out to obtain a high-purity natural betaine crystalline product with the purity of 95-99.9%, and then the product is dried and packaged to obtain a commercial finished product.
The crystallization mother liquor can be circulated to the front of the continuous chromatographic system, and the clear liquid filtered by the mixed membrane is used as the feeding material of the chromatographic system, so that the overall yield is further improved.
The process adopts yeast wastewater containing betaine as a raw material, usually yeast wastewater concentrate based on beet molasses, wherein the dry basis content of natural betaine in the wastewater reaches 10-15%, the wastewater is waste in environmental protection treatment, the raw material cost is low, a series of separation technologies are combined, the process typically comprises a physical or chemical primary pretreatment, ultrafiltration membrane deep pretreatment, one-step or multi-step chromatographic separation technology, active decolorization and crystallization coupling process, the betaine is effectively recycled, as the fermentation broth wastewater contains a large amount of viscous substances such as polysaccharides, proteins and other impurities and colloidal substances, the fermentation broth wastewater needs to be fully pretreated firstly to be further separated and purified, the pretreatment process can be centrifuge or chemical flocculation or chemical precipitation reaction treatment, and the combination of the above treatment processes, the deep pretreatment uses a molecular-level ultrafiltration membrane for pre-impurity removal, and introducing a chromatography process, wherein the purification and impurity removal are carried out by one-step or multi-step chromatography depending on the quality of the final betaine, then activated carbon or resin or a nanofiltration membrane is used for decolorization, and finally, a high-purity natural betaine crystal product with the purity of 95-99.9% is obtained by evaporation concentration and crystallization.
The adopted ceramic membrane ultrafiltration, continuous chromatographic separation and activated carbon decoloration are physical separation, chromatographic resin separation utilizes the small molecular size of betaine and has long retention time in resin, while salt in yeast wastewater has short retention time in resin through an ion exclusion mechanism (which is just opposite to ion exchange), and macromolecules such as residual sugar in the yeast wastewater are separated through a size exclusion mechanism and have short retention time in resin; the chromatographic separation process only uses pure water, and the mode retrieval and utilization of the pure water accessible evaporation condensation that this part added, whole chromatographic process need not additionally consume any chemicals, high-efficient separation through chromatography process, can directly obtain natural betaine product, and the use of chemicals has been reduced by a wide margin, with the technology of patent CN10493333 a large amount of chemicals production betaine hydrochloride, there is essential difference, the waste liquid of production can not bring extra burden to downstream sewage treatment, can effectual active ingredient in the recycle waste liquid, the burden of sewage treatment has still been alleviateed.
Meanwhile, potassium ions in the wastewater are also reserved in the raffinate after the extraction of the betaine, so that the method brings a beneficial value to factories, also brings other benefits to downstream environmental protection, and can be further used for producing high-quality organic fertilizers or extracting potassium salts (usually potassium sulfate).
Example 2: method for extracting natural betaine from yeast wastewater
A method for extracting natural betaine from yeast wastewater comprises the following steps:
step 1: carrying out impurity removal pretreatment on the yeast wastewater;
step 2: introducing the pretreated liquid obtained in the step 2 into an ultrafiltration membrane system, and performing cross-flow filtration under the pressure of 3 kilograms to obtain filtrate and concentrated solution which is 5 times concentrated;
and step 3: introducing the filtrate obtained in the step 2 into a continuous chromatography system to obtain an extract component rich in betaine and a raffinate component rich in salt and other impurities;
and 4, step 4: and (3) decoloring and concentrating the extracting solution rich in betaine to obtain a betaine liquid product with the purity of 65% by mass fraction.
The preferred embodiment is: the impurity removal pretreatment comprises centrifugal separation, wherein the centrifugal rotation speed is 3500 r/min.
The preferred embodiment is: the ultrafiltration membrane system adopts a ceramic membrane or an organic membrane, the filtration precision is 1 ten thousand daltons, and the filtration temperature is 55 ℃.
The preferred embodiment is: the continuous chromatography system consists of 6 chromatography columns. Continuous chromatography systems may also be referred to as continuous flow chromatography systems.
The preferred embodiment is: the chromatographic column is filled with cation exchange resin which is in a sodium type, a potassium type, a calcium type or a hydrogen type; the particle size of the cation exchange resin is 200-500 microns. The cation exchange resin can be specifically strong acid styrene series cation exchange resin, strong acid acrylic series cation exchange resin, such as 001x7 type cation exchange resin, MDS1368 series or CR99 series.
The preferred embodiment is: in the step 3, the mass concentration of the feeding material during chromatography is 40%, the working temperature is 60 ℃, and the feeding flow rate is 4 m/h.
Example 3: method for extracting natural betaine from yeast wastewater
A method for extracting natural betaine from yeast wastewater comprises the following steps:
step 1: carrying out impurity removal pretreatment on the yeast wastewater;
step 2: introducing the pretreated liquid obtained in the step 2 into an ultrafiltration membrane system, and performing cross-flow filtration under the pressure of 1 kg to obtain concentrated solution and filtrate which are concentrated by 3 times;
and step 3: introducing the filtrate obtained in the step (2) into a continuous chromatography system to obtain an extract component rich in betaine and a raffinate component rich in salt and other impurities;
and 4, step 4: cooling and crystallizing or evaporating and crystallizing the extracting solution rich in betaine to obtain a high-purity natural betaine crystal product with the purity of 95%.
The preferred embodiment is: the impurity removal pretreatment comprises a mode of combining chemical flocculation and centrifugal separation. The flocculant may be polyacrylamide or polyaluminium sulfate.
The preferred embodiment is: the ultrafiltration membrane system adopts a ceramic membrane or an organic membrane, the filtration precision is 1000 daltons, and the filtration temperature is 30 ℃.
The preferred embodiment is: the continuous chromatography system consists of 4 chromatography columns.
The preferred embodiment is: the chromatographic column is filled with cation exchange resin which is in a sodium type, a potassium type, a calcium type or a hydrogen type; the particle size of the cation exchange resin was 220 microns.
The preferred embodiment is: in the step 3, the mass concentration of the feeding material during chromatography is 20%, the working temperature is 40 ℃, and the feeding flow rate is 1 m/h.
Example 4: method for extracting natural betaine from yeast wastewater
A method for extracting natural betaine from yeast wastewater comprises the following steps:
step 1: carrying out impurity removal pretreatment on the yeast wastewater;
step 2: introducing the pretreated liquid obtained in the step 2 into an ultrafiltration membrane system, and performing cross-flow filtration under the pressure of 5 kilograms to obtain concentrated solution and filtrate which are concentrated by 10 times;
and step 3: introducing the filtrate obtained in the step 2 into a continuous chromatography system to obtain an extract component rich in betaine and a raffinate component rich in salt and other impurities;
and 4, step 4: decoloring and concentrating the extracting solution rich in betaine to obtain a betaine liquid product with the purity of 85% by mass; or cooling and crystallizing or evaporating and crystallizing the extracting solution rich in betaine to obtain a high-purity natural betaine crystal product with the purity of 99%.
The preferred embodiment is: the impurity removal pretreatment comprises at least one of centrifugal separation, chemical flocculation or chemical precipitation reaction.
The preferred embodiment is: the ultrafiltration membrane system adopts a ceramic membrane or an organic membrane, the filtration precision is 50 ten thousand daltons, and the filtration temperature is 85 ℃.
The preferred embodiment is: the continuous chromatography system consists of 10 chromatography columns.
The preferred embodiment is: the chromatographic column is filled with cation exchange resin which is in a sodium type, a potassium type, a calcium type or a hydrogen type; the particle size of the cation exchange resin was 350. mu.m.
The preferred embodiment is: in the step 3, the mass concentration of the feeding material during chromatography is 60%, the working temperature is 80 ℃, and the feeding flow rate is 8 m/h.
The foregoing is illustrative of the preferred embodiment of the present invention and is not to be construed as limiting thereof in any way, and any modifications or variations thereof that fall within the spirit of the invention are intended to be included within the scope thereof.
Claims (6)
1. A method for extracting natural betaine from yeast wastewater is characterized by comprising the following steps: comprises the following steps:
step 1: carrying out impurity removal pretreatment on the yeast wastewater;
step 2: introducing the liquid obtained after pretreatment in the step 2 into an ultrafiltration membrane system, and performing cross-flow filtration under the pressure of 1-5 kg to obtain a filtrate;
and step 3: introducing the filtrate obtained in the step 2 into a continuous chromatography system to obtain an extract component rich in betaine and a raffinate component rich in salt and other impurities;
and 4, step 4: decoloring and concentrating the extracting solution rich in betaine to obtain a betaine liquid product with the mass fraction of 50-85%; or cooling and crystallizing or evaporating the extract rich in betaine to obtain high-purity natural betaine crystal product with purity of 95-99.9%.
2. The method for extracting natural betaine from yeast wastewater as claimed in claim 1, wherein the method comprises the following steps: the impurity removal pretreatment comprises at least one of centrifugal separation, chemical flocculation or chemical precipitation reaction.
3. The method for extracting natural betaine from yeast wastewater as claimed in claim 1, wherein the method comprises the following steps: the ultrafiltration membrane system adopts ceramic membrane or organic membrane, the filtration precision is 1000 Dalton-50 ten thousand Dalton, and the filtration temperature is 30-85 deg.C.
4. The method for extracting natural betaine from yeast wastewater as claimed in claim 1, wherein the method comprises the following steps: the continuous chromatography system consists of 4-10 chromatography columns.
5. The method for extracting natural betaine from yeast wastewater as claimed in claim 4, wherein the method comprises the following steps: the chromatographic column is filled with cation exchange resin which is in a sodium type, a potassium type, a calcium type or a hydrogen type; the particle size of the cation exchange resin is 200-500 microns.
6. The method for extracting natural betaine from yeast wastewater as claimed in claim 1, wherein the method comprises the following steps: in the step 3, the mass concentration of the feeding material during chromatography is 20-60%, the working temperature is 40-80 ℃, and the feeding flow rate is 1-10 m/h.
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| CN202210619990.1A CN114957026A (en) | 2022-06-02 | 2022-06-02 | Method for extracting natural betaine from yeast wastewater |
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| CN202210619990.1A CN114957026A (en) | 2022-06-02 | 2022-06-02 | Method for extracting natural betaine from yeast wastewater |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1117967A (en) * | 1994-08-27 | 1996-03-06 | 房有树 | Technology for extracting betaine hydrochloride |
| CN103570569A (en) * | 2013-10-17 | 2014-02-12 | 华南理工大学 | Method for preparing glycine betaine by using beet ethyl alcohol waste liquor |
| CN105566137A (en) * | 2015-12-25 | 2016-05-11 | 安徽丰原发酵技术工程研究有限公司 | Method for separation and purification of betaine from beet molasses |
| CN107602404A (en) * | 2017-09-25 | 2018-01-19 | 新疆绿原糖业有限公司 | A kind of method from molasses alcohol waste liquid extraction high-purity betaine |
| CN112679372A (en) * | 2020-12-18 | 2021-04-20 | 新疆冠农果茸股份有限公司 | Process for recovering betaine from beet waste honey |
-
2022
- 2022-06-02 CN CN202210619990.1A patent/CN114957026A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1117967A (en) * | 1994-08-27 | 1996-03-06 | 房有树 | Technology for extracting betaine hydrochloride |
| CN103570569A (en) * | 2013-10-17 | 2014-02-12 | 华南理工大学 | Method for preparing glycine betaine by using beet ethyl alcohol waste liquor |
| CN105566137A (en) * | 2015-12-25 | 2016-05-11 | 安徽丰原发酵技术工程研究有限公司 | Method for separation and purification of betaine from beet molasses |
| CN107602404A (en) * | 2017-09-25 | 2018-01-19 | 新疆绿原糖业有限公司 | A kind of method from molasses alcohol waste liquid extraction high-purity betaine |
| CN112679372A (en) * | 2020-12-18 | 2021-04-20 | 新疆冠农果茸股份有限公司 | Process for recovering betaine from beet waste honey |
Non-Patent Citations (1)
| Title |
|---|
| 蔡春林等: "膜技术在糖蜜酒精废液处理中的应用", 《广州化工》, vol. 38, no. 10, pages 2 - 3 * |
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