CN114956909A - Soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization - Google Patents
Soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization Download PDFInfo
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- CN114956909A CN114956909A CN202210809198.2A CN202210809198A CN114956909A CN 114956909 A CN114956909 A CN 114956909A CN 202210809198 A CN202210809198 A CN 202210809198A CN 114956909 A CN114956909 A CN 114956909A
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- 239000003516 soil conditioner Substances 0.000 title claims abstract description 56
- 230000012010 growth Effects 0.000 title claims abstract description 35
- 244000005700 microbiome Species 0.000 title claims abstract description 31
- 238000005457 optimization Methods 0.000 title claims abstract description 28
- 230000001737 promoting effect Effects 0.000 title claims abstract description 27
- 241000976738 Bacillus aryabhattai Species 0.000 claims abstract description 60
- 241000223250 Metarhizium anisopliae Species 0.000 claims abstract description 45
- 241000223259 Trichoderma Species 0.000 claims abstract description 44
- 238000000855 fermentation Methods 0.000 claims abstract description 43
- 230000004151 fermentation Effects 0.000 claims abstract description 43
- 230000000694 effects Effects 0.000 claims abstract description 35
- 239000010871 livestock manure Substances 0.000 claims abstract description 33
- 239000002361 compost Substances 0.000 claims abstract description 30
- 239000000843 powder Substances 0.000 claims abstract description 23
- 230000006872 improvement Effects 0.000 claims abstract description 22
- 230000000813 microbial effect Effects 0.000 claims abstract description 15
- 241000233866 Fungi Species 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims description 30
- 238000012258 culturing Methods 0.000 claims description 27
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 20
- 210000003608 fece Anatomy 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 19
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- 239000007788 liquid Substances 0.000 claims description 13
- 238000009630 liquid culture Methods 0.000 claims description 12
- 239000011248 coating agent Substances 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 10
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 9
- 238000007865 diluting Methods 0.000 claims description 9
- 239000004021 humic acid Substances 0.000 claims description 9
- 230000003213 activating effect Effects 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 7
- 240000000528 Ricinus communis Species 0.000 claims description 7
- 235000004443 Ricinus communis Nutrition 0.000 claims description 7
- 229930013930 alkaloid Natural products 0.000 claims description 7
- 150000003797 alkaloid derivatives Chemical class 0.000 claims description 7
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 7
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 7
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000010009 beating Methods 0.000 claims description 6
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- 239000000203 mixture Substances 0.000 claims description 6
- 241000361919 Metaphire sieboldi Species 0.000 claims description 5
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- 235000000340 Solanum pseudocapsicum Nutrition 0.000 claims description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
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- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
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- 235000015278 beef Nutrition 0.000 claims description 3
- 244000144972 livestock Species 0.000 claims description 3
- 238000000643 oven drying Methods 0.000 claims description 3
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- 239000012137 tryptone Substances 0.000 claims description 3
- 241001494479 Pecora Species 0.000 claims description 2
- 241001465382 Physalis alkekengi Species 0.000 claims description 2
- 239000002364 soil amendment Substances 0.000 claims 8
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- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 102000016938 Catalase Human genes 0.000 description 3
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- 108010046334 Urease Proteins 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- 241000223260 Trichoderma harzianum Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
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- 239000000047 product Substances 0.000 description 2
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- 238000011160 research Methods 0.000 description 2
- VRZJGENLTNRAIG-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]iminonaphthalen-1-one Chemical compound C1=CC(N(C)C)=CC=C1N=C1C2=CC=CC=C2C(=O)C=C1 VRZJGENLTNRAIG-UHFFFAOYSA-N 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
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- 235000015097 nutrients Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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- 239000002881 soil fertilizer Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Organic Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
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- Tropical Medicine & Parasitology (AREA)
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- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization, which comprises the following components in parts by weight: 40-100 parts of livestock manure compost, 1-10 parts of Bacillus aryabhattai active spore powder, 10-40 parts of trichoderma fermentation liquor, 10-40 parts of Metarrhizium anisopliae fermentation liquor and 10-30 parts of activity improvement synergist. The invention belongs to the technical field of soil conditioners, and provides a method for combining biocontrol fungi with beneficial soil bacteria to realize synergistic interaction, so that the technical effects of enhancing the biological control effect and improving the physical and chemical properties of soil are realized, and meanwhile, the growth speed and the adaptability of microbial flora are improved due to the arrangement of an active improvement synergist, so that the soil conditioner can be suitable for the soil in various different environments, and the application universality of the soil conditioner is improved.
Description
Technical Field
The invention belongs to the technical field of soil conditioners, and particularly relates to a soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization.
Background
The soil conditioner is mainly used for improving the physical, chemical and biological properties of soil, so that the soil conditioner is more suitable for plant growth, usually comprises a chemical soil conditioner and a biological soil conditioner, and because the content of soil exceeds the standard due to long-term use of chemical substances, the green development of agriculture is promoted, and the reduction and the synergism of chemical fertilizers and pesticides become important.
In the development of microbial fertilizers and microbial pesticides, because the spore-free bacterial fertilizers are not resistant to high temperature and drying in production, most of prepared products are not resistant to storage, so that the selection of bacillus with strong stress resistance and capability of being stored for a long time is a key direction for the development of the bacterial fertilizers in the future. Compared with bacterial trophosome, the spore has stronger tolerance, so the product has more advantages in the aspects of production, processing, storage, transportation, application range and the like, and is more biased to the bacillus in the selection of strains in the development of microbial pesticides, so that the bacillus with strong stress resistance and long-term storage is always the key point of concern in the research of microbial pesticides and fertilizers, for example, the bacillus thuringiensis can be prepared into an insecticide, and the bacillus megaterium can be prepared into a phosphate solubilizing bacterial fertilizer, but the research core is still the screening of strains with excellent performance, and the following problems mainly exist in the prior art at present: the existing microbial soil conditioner has low control effect; the existing soil conditioner and biological fertilizer are mostly used separately, and the degradation effect of beneficial microorganisms in the soil conditioner on the fertilizer cannot be fully utilized; secondly, due to the diversity of microbial floras, the prior art does not fully realize the action relationship between fungi and bacteria in the soil conditioner so as to realize the function of synergy.
Disclosure of Invention
Aiming at the situation and overcoming the defects of the prior art, the invention provides a soil conditioner for promoting the growth of microorganisms and realizing the biological control and rational optimization, in order to solve the problem of low control effect of the existing microbial soil conditioner, the invention provides a method for realizing synergistic interaction by combining biocontrol fungi and beneficial bacteria in soil, thereby realizing the technical effects of enhancing the biological control effect and improving the physical and chemical properties of soil, improving the growth speed and the adaptability of microbial flora by the arrangement of the activity improvement synergist, the soil conditioner provided by the invention can not only improve the number of floras of beneficial organisms in the soil, but also improve the physicochemical property, and has important reference value and application value for improving the soil improved by microorganisms.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: the invention provides a soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization, which comprises the following components in parts by weight:
40-100 parts of livestock manure compost;
1-10 parts of live spore powder of Bacillus aryabhattai;
10-40 parts of trichoderma fermentation liquor;
10-40 parts of Metarrhizium anisopliae fermentation liquor;
10-30 parts of activity improvement synergist.
Preferably, the soil conditioner comprises the following components in parts by weight:
50-100 parts of livestock manure compost;
2-8 parts of Bacillus aryabhattai live spore powder;
10-20 parts of trichoderma fermentation liquor;
10-20 parts of Metarrhizium anisopliae fermentation liquor;
10-25 parts of activity improvement synergist.
Further, the livestock manure compost comprises one or more of cow manure compost, sheep manure compost, horse manure compost and earthworm manure compost.
Further, the activity improvement synergist comprises the following components in parts by weight: 15-40 parts of oyster shell, 5-10 parts of physalis alkekengi, 1-8 parts of calcium superphosphate, 10-30 parts of humic acid, 1-10 parts of alkaloid, 1-8 parts of magnesium sulfate and 5-15 parts of castor bean.
Preferably, the activity improvement synergist comprises the following components in parts by weight: 20-30 parts of oyster shell, 8-10 parts of wintercherry, 5-8 parts of calcium superphosphate, 15-30 parts of humic acid, 5-10 parts of alkaloid, 1-5 parts of magnesium sulfate and 10-15 parts of castor bean.
The invention also provides a preparation method of the soil conditioner for promoting the growth of microorganisms to realize the biological control and rational optimization, which specifically comprises the following steps:
(1) preparing live spore powder of Bacillus aryabhattai, trichoderma fermentation liquor and Metarrhizium anisopliae fermentation liquor;
(2) fully mixing the live spore powder of the bacillus aryabhattai, the trichoderma fermentation liquid and the green muscardine fermentation liquid with the livestock and poultry manure compost to obtain a biological fertilizer composition;
(3) and fully mixing the activity improvement synergist with the biological fertilizer composition to obtain the soil conditioner.
Preferably, the preparation method of the live spore powder of the bacillus aryabhattai comprises the following steps:
(1) activating bacillus aryabhattai: diluting Bacillus aryabhattai by 5-10 times, coating on an activated solid culture medium, and culturing at 37 ℃ for 12 h to obtain activated Bacillus aryabhattai;
(2) subculturing of Bacillus aryabhattai: selecting a single colony of the activated Bacillus aryabhattai in 500 mL of propagation liquid culture medium by using an inoculating needle, and culturing at 37 ℃ and 180 rpm for 12 h to obtain first-grade Bacillus aryabhattai;
(3) b, bacillus aryabhattai proliferation culture: 5 mL of the first-grade Bacillus aryabhattai in the second step is sucked into a propagation liquid culture medium, and is cultured for 24-48 h at 37 ℃ and 180 rpm to obtain a Bacillus aryabhattai active liquid;
(4) preparing live Bacillus aryabhattai powder: centrifuging the Bacillus aryabhattai active liquid at 150 rpm for 1 min, removing supernatant to obtain Bacillus aryabhattai thallus, and oven drying at 30-37 deg.C for 30-60 min to obtain Bacillus aryabhattai active spore powder.
Preferably, the activated solid medium is prepared from the following components in percentage by weight: : 6-10% of potato dregs, 0.1-0.4% of beef extract, 0.1-1.5% of soytone, 0.1-1.5% of tryptone, 0.2-0.6% of sodium chloride, 0.2-0.5% of magnesium sulfate, 2.5-4.5% of agar and the balance of water, wherein the pH value is 7.1-7.5.
Preferably, the preparation method of the trichoderma fermentation liquor comprises the following steps:
(1) activating trichoderma: diluting conidia of trichoderma harzianum by 5-10 times, coating on PDA solid culture medium, and culturing at 28 deg.C for 7 d to obtain activated trichoderma harzianum;
(2) subculturing trichoderma: picking the fungus block of the activated trichoderma by using an inoculating needle, and culturing for 7 d at 28 ℃ in a PDA solid culture medium to obtain first-grade trichoderma;
(3) and (3) fermenting and culturing trichoderma: and (4) beating the fungus block of the primary trichoderma in the step two in a PDB liquid culture medium, and culturing at 28 ℃ and 150 rpm for 56-72 h to obtain trichoderma fermentation liquor.
Preferably, the preparation method of the metarhizium anisopliae fermentation liquor comprises the following steps:
(1) activating metarhizium anisopliae: diluting conidia of Metarrhizium anisopliae by 5-10 times, coating on PDA solid culture medium, and culturing at 28 deg.C for 7 d to obtain activated Metarrhizium anisopliae;
(2) subculturing Metarrhizium anisopliae: selecting activated metarhizium anisopliae in a PDA solid culture medium by using an inoculating needle, and culturing at 28 ℃ for 7 d to obtain first-grade metarhizium anisopliae;
(3) and (3) fermentation culture of Metarrhizium anisopliae: and (4) beating the fungus block of the first-level metarhizium anisopliae in the step two in a PDB liquid culture medium, and culturing at 28 ℃ and 150 rpm for 56-72 h to obtain metarhizium anisopliae fermentation liquor.
The invention with the structure has the following beneficial effects:
(1) in order to solve the problem of low control effect of the existing microbial soil conditioner, the invention provides a technical effect of enhancing the biological control effect and improving the physical and chemical properties of soil by combining the biocontrol fungi with beneficial bacteria in the soil;
(2) the secretion of the Bacillus aryabhattai can promote the growth of the fungi trichoderma and the fungi metarhizium anisopliae, and the fungi trichoderma and the fungi metarhizium anisopliae have the function of preventing and treating various pathogenic microorganisms, so that the function of synergy is realized;
(3) meanwhile, the growth speed and the adaptability of microbial flora are improved due to the arrangement of the activity improvement synergist, so that the soil conditioner can be suitable for soil in various environments, and the application universality of the soil conditioner is improved;
(4) humic acid is a common soil fertilizer, and through further degradation of Bacillus aryabhattai, the nutrient required by growth and development of the humic acid is provided, the propagation of soil microorganisms in an applied soil environment is promoted, and the diversity of the microorganisms in the soil is improved;
(5) the soil conditioner provided by the invention can improve the number of floras of beneficial organisms in soil and improve physicochemical properties, and has important reference value and application value for improving soil by microorganisms.
Drawings
FIG. 1 is a result diagram of plant height after treatment with a soil conditioner for promoting growth of microorganisms to achieve both biological control and rational optimization;
FIG. 2 is a result graph of the volume weight of soil after treatment with a soil conditioner for promoting growth of microorganisms to achieve rational optimization of biological control;
FIG. 3 is a result graph of soil urease size after treatment with soil conditioner for microbial growth promotion and physiological optimization of biocontrol;
FIG. 4 is a graph of results of soil catalase treatment with soil conditioner for microbial growth promotion for rational optimization of biocontrol;
FIG. 5 is a result chart of the number of pathogenic bacteria after treatment with a soil conditioner for microbial growth promotion and rational optimization of biocontrol.
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The experimental methods in the following examples are all conventional methods unless otherwise specified; the test materials and test strains used in the following examples were all commercially available unless otherwise specified, and specifically, the metarhizium anisopliae used in the examples of the present invention was commercially available metarhizium anisopliae ACCC30194, the Bacillus aryabhattai was commercially available Bacillus aryabhattai CICC 2474, and the xylanase was commercially available Trichoderma viride CGMCC 3.2942.
Example 1
Soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization
The soil conditioner comprises the following components in parts by weight:
100 parts of livestock manure compost;
8 parts of live spore powder of Bacillus aryabhattai;
15 parts of trichoderma fermentation liquor;
15 parts of Metarrhizium anisopliae fermentation liquor;
25 parts of activity improvement synergist.
Wherein the livestock manure compost comprises cow manure compost, horse manure compost and earthworm manure according to the proportion of 1: 2: 1, carrying out combined preparation.
Wherein the activity improvement synergist comprises the following components in parts by weight: 20-30 parts of oyster shell, 8-10 parts of wintercherry, 5-8 parts of calcium superphosphate, 15-30 parts of humic acid, 5-10 parts of alkaloid, 1-5 parts of magnesium sulfate and 10-15 parts of castor bean.
The invention also provides a preparation method of the soil conditioner for promoting the growth of microorganisms to realize the biological control and rational optimization, which specifically comprises the following steps:
(1) preparing live spore powder of Bacillus aryabhattai, trichoderma fermentation liquor and Metarrhizium anisopliae fermentation liquor;
(2) fully mixing the live spore powder of the bacillus aryabhattai, the trichoderma fermentation liquid and the green muscardine fermentation liquid with the livestock and poultry manure compost to obtain a biological fertilizer composition;
(3) and fully mixing the activity improvement synergist with the biological fertilizer composition to obtain the soil conditioner.
Preferably, the preparation method of the live spore powder of the bacillus aryabhattai comprises the following steps:
(1) activating bacillus aryabhattai: diluting Bacillus aryabhattai by 5-10 times, coating on an activated solid culture medium, and culturing at 37 ℃ for 12 h to obtain activated Bacillus aryabhattai;
(2) subculturing of Bacillus aryabhattai: selecting a single colony of activated Bacillus aryabhattai in a 500 mL propagation liquid culture medium by using an inoculating needle, and culturing at 37 ℃ and 180 rpm for 12 h to obtain first-grade Bacillus aryabhattai;
(3) b, bacillus aryabhattai proliferation culture: 5 mL of the first-grade Bacillus aryabhattai in the second step is sucked into a propagation liquid culture medium, and is cultured for 24-48 h at 37 ℃ and 180 rpm to obtain a Bacillus aryabhattai active liquid;
(4) preparing live bacillus aryabhattai powder: centrifuging the Bacillus aryabhattai active liquid at 150 rpm for 1 min, removing supernatant to obtain Bacillus aryabhattai thallus, and oven drying at 30-37 deg.C for 30-60 min to obtain Bacillus aryabhattai active spore powder.
Wherein the activated solid culture medium is prepared from the following components in percentage by weight: : 6-10% of potato dregs, 0.1-0.4% of beef extract, 0.1-1.5% of soytone, 0.1-1.5% of tryptone, 0.2-0.6% of sodium chloride, 0.2-0.5% of magnesium sulfate, 2.5-4.5% of agar and the balance of water, wherein the pH value is 7.1-7.5.
In addition, the preparation method of the trichoderma fermentation liquor comprises the following steps:
(1) activating trichoderma: diluting trichoderma conidium by 5-10 times, coating on PDA solid culture medium, and culturing at 28 deg.C for 7 days to obtain activated trichoderma;
(2) subculturing trichoderma: picking the fungus block of the activated trichoderma by using an inoculating needle, and culturing for 7 d at 28 ℃ in a PDA solid culture medium to obtain first-grade trichoderma;
(3) and (3) fermenting and culturing trichoderma: and (4) beating the fungus block of the primary trichoderma in the step two in a PDB liquid culture medium, and culturing at 28 ℃ and 150 rpm for 56-72 h to obtain trichoderma fermentation liquor.
The preparation method of the Metarrhizium anisopliae fermentation liquid comprises the following steps:
(1) activating metarhizium anisopliae: diluting conidia of Metarrhizium anisopliae by 5-10 times, coating on PDA solid culture medium, and culturing at 28 deg.C for 7 d to obtain activated Metarrhizium anisopliae;
(2) carrying out subculture on metarhizium anisopliae: selecting activated metarhizium anisopliae in a PDA solid culture medium by using an inoculating needle, and culturing at 28 ℃ for 7 d to obtain first-grade metarhizium anisopliae;
(3) and (3) fermentation culture of Metarrhizium anisopliae: and (3) beating the fungus block of the first-level metarhizium anisopliae in the step two into a PDB liquid culture medium, and culturing at 28 ℃ and 150 rpm for 56-72 h to obtain metarhizium anisopliae fermentation liquor.
Example 2
Soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization
The soil conditioner comprises the following components in parts by weight:
100 parts of livestock manure compost;
6 parts of Bacillus aryabhattai live spore powder;
15 parts of trichoderma fermentation liquor;
15 parts of Metarrhizium anisopliae fermentation liquor;
30 parts of an activity improvement synergist.
Wherein the livestock manure compost comprises cow manure compost, horse manure compost and earthworm manure according to the proportion of 1: 2: 1, carrying out combined preparation.
Wherein the activity improvement synergist comprises the following components in parts by weight: 20-30 parts of oyster shell, 8-10 parts of wintercherry, 5-8 parts of calcium superphosphate, 15-30 parts of humic acid, 5-10 parts of alkaloid, 1-5 parts of magnesium sulfate and 10-15 parts of castor bean.
In addition, the invention also provides a preparation method of the soil conditioner for promoting the growth of microorganisms to realize the physiological optimization of biological control, and the preparation method is implemented according to the embodiment 1
Example 3
Soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization
The soil conditioner comprises the following components in parts by weight:
100 parts of livestock manure compost;
8 parts of live spore powder of Bacillus aryabhattai;
20 parts of trichoderma fermentation liquor;
20 parts of Metarrhizium anisopliae fermentation liquor;
20 parts of activity improvement synergist.
Wherein the livestock manure compost comprises cow manure compost, horse manure compost and earthworm manure according to the proportion of 1: 2: 1, carrying out combined preparation.
Wherein the activity improvement synergist comprises the following components in parts by weight: 20-30 parts of oyster shell, 8-10 parts of wintercherry, 5-8 parts of calcium superphosphate, 15-30 parts of humic acid, 5-10 parts of alkaloid, 1-5 parts of magnesium sulfate and 10-15 parts of castor bean.
In addition, the invention also provides a preparation method of the soil conditioner for promoting the growth of microorganisms to realize the physiological optimization of biological control, and the preparation method is implemented according to the embodiment 1
Comparative example 1
The present comparative example provides a soil conditioner for promoting growth of microorganisms to achieve rational optimization of biocontrol and a method for preparing the same, which is different from example 1 only in that all components do not contain an active improvement synergist and the rest components and the content of the components are the same as example 1.
Examples of the experiments
Soil conditioner for improving cucumber planting field by using soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization
In the experimental example, the cucumber planting field is selected as the test field, the soil is poor due to continuous cropping of the cucumber, the water storage property is poor, the accumulation amount of pathogenic bacteria is large, and the test specifically comprises the following steps in order to improve the soil of the test field:
(1) a soil conditioner was prepared with reference to example 1, example 2, example 3 and comparative example 1;
(2) the method comprises the following steps that after soil conditioner is applied to a test group, the soil is turned over and stirred, cucumber seedlings are planted after furrows are made smoothly, the soil conditioner is not applied to the test group and used as a blank control, other planting methods, environments and management methods of the test group and the control group are kept the same, and the test group and the control group are respectively provided with 3 repetitions;
(3) counting the plant heights of the test group and the control group after 30 days, and testing indexes such as soil volume weight, soil enzyme activity and the like, wherein the soil urease activity is measured by an indophenol blue colorimetric method, and the soil catalase activity is measured by an ultraviolet spectrophotometry;
(4) collecting soil of the test group and the control group by adopting a five-point sampling method, counting cucumber fusarium wilt bacteria of cucumber pathogenic bacteria by adopting a flat plate coating counting method, and identifying the number of the cucumber fusarium wilt bacteria by using a Komada selective culture medium.
Analysis of results
(1) Influence of soil conditioner on soil physicochemical property
As shown in figure 1, figure 2, figure 3 and figure 4, the soil conditioner provided by the invention can reduce the soil capacity and improve the growth of plants, and can improve the soil urease activity and reduce the soil catalase activity.
(2) Influence of soil conditioner on cucumber pathogenic bacteria
As shown in fig. 5, the soil conditioner can reduce the number of cucumber pathogenic bacteria, thereby reducing the cucumber morbidity.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The present invention and its embodiments have been described above, and the description is not intended to be limiting, and what is shown in the drawings is only one embodiment of the present invention, and the practical application is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A soil conditioner for promoting growth of microorganisms to realize biological control and rational optimization is characterized in that: the soil conditioner comprises the following components in parts by weight:
40-100 parts of livestock manure compost;
1-10 parts of live spore powder of Bacillus aryabhattai;
10-40 parts of trichoderma fermentation liquor;
10-40 parts of Metarrhizium anisopliae fermentation liquor;
10-30 parts of activity improvement synergist.
2. The soil conditioner for promoting microbial growth and achieving biological control and rational optimization according to claim 1, is characterized in that: the soil conditioner comprises the following components in parts by weight:
50-100 parts of livestock manure compost;
2-8 parts of Bacillus aryabhattai live spore powder;
10-20 parts of trichoderma fermentation liquor;
10-20 parts of Metarrhizium anisopliae fermentation liquor;
10-25 parts of activity improvement synergist.
3. A soil amendment according to claim 2 for promoting growth of microorganisms for achieving a combination of biological control and rational optimisation, the soil amendment comprising: the livestock manure compost comprises one or more of cow manure compost, sheep manure compost, horse manure compost and earthworm manure compost.
4. A soil amendment according to claim 3 for promoting growth of microorganisms for achieving simultaneous optimization of biological control and rationality, characterized in that: the activity improvement synergist comprises the following components in parts by weight: 15-40 parts of oyster shell, 5-10 parts of physalis alkekengi, 1-8 parts of calcium superphosphate, 10-30 parts of humic acid, 1-10 parts of alkaloid, 1-8 parts of magnesium sulfate and 5-15 parts of castor bean.
5. A soil amendment according to claim 4 for promoting growth of microorganisms for achieving simultaneous optimization of biological control and rationality, characterized in that: the activity improvement synergist comprises the following components in parts by weight: 20-30 parts of oyster shell, 8-10 parts of wintercherry, 5-8 parts of calcium superphosphate, 15-30 parts of humic acid, 5-10 parts of alkaloid, 1-5 parts of magnesium sulfate and 10-15 parts of castor bean.
6. A method for preparing a soil conditioner for promoting the growth of microorganisms to realize the rational optimization of biological control according to claim 1, which is characterized in that: the preparation method comprises the following steps:
(1) preparing live spore powder of Bacillus aryabhattai, trichoderma fermentation liquor and Metarrhizium anisopliae fermentation liquor;
(2) fully mixing the live spore powder of the bacillus aryabhattai, the trichoderma fermentation liquid and the green muscardine fermentation liquid with the livestock and poultry manure compost to obtain a biological fertilizer composition;
(3) and fully mixing the activity improvement synergist with the biological fertilizer composition to obtain the soil conditioner.
7. A soil amendment according to claim 6 for promoting growth of microorganisms for achieving simultaneous optimization of biological control and rationality, characterized in that: the preparation method of the live spore powder of the Bacillus aryabhattai comprises the following steps:
(1) activating bacillus aryabhattai: diluting Bacillus aryabhattai by 5-10 times, coating on an activated solid culture medium, and culturing at 37 ℃ for 12 h to obtain activated Bacillus aryabhattai;
(2) subculturing of Bacillus aryabhattai: selecting a single colony of activated Bacillus aryabhattai in a 500 mL propagation liquid culture medium by using an inoculating needle, and culturing at 37 ℃ and 180 rpm for 12 h to obtain first-grade Bacillus aryabhattai;
(3) b, bacillus aryabhattai propagation culture: 5 mL of the first-grade Bacillus aryabhattai in the second step is sucked into a propagation liquid culture medium, and is cultured for 24-48 h at 37 ℃ and 180 rpm to obtain a Bacillus aryabhattai active liquid;
(4) preparing live bacillus aryabhattai powder: centrifuging the Bacillus aryabhattai active liquid at 150 rpm for 1 min, removing supernatant to obtain Bacillus aryabhattai thallus, and oven drying at 30-37 deg.C for 30-60 min to obtain Bacillus aryabhattai active spore powder.
8. A soil amendment according to claim 7 for promoting growth of microorganisms for achieving simultaneous optimization of biological control and rationality, characterized in that: the activated solid culture medium is prepared from the following components in percentage by weight: 6-10% of potato dregs, 0.1-0.4% of beef extract, 0.1-1.5% of soytone, 0.1-1.5% of tryptone, 0.2-0.6% of sodium chloride, 0.2-0.5% of magnesium sulfate, 2.5-4.5% of agar and the balance of water, wherein the pH value is 7.1-7.5.
9. A soil amendment according to claim 8 for promoting growth of microorganisms for achieving simultaneous optimization of biological control and rationality, characterized in that: the preparation method of the trichoderma fermentation liquor comprises the following steps:
(1) and (3) activation of trichoderma: diluting trichoderma conidium by 5-10 times, coating on PDA solid culture medium, and culturing at 28 deg.C for 7 days to obtain activated trichoderma;
(2) subculturing trichoderma: picking the fungus block of the activated trichoderma by using an inoculating needle, and culturing for 7 d at 28 ℃ in a PDA solid culture medium to obtain first-grade trichoderma;
(3) and (3) fermenting and culturing trichoderma: and (4) beating the fungus block of the first-level trichoderma in the step two in a PDB liquid culture medium, and culturing at 28 ℃ and 150 rpm for 56-72 h to obtain trichoderma fermentation liquor.
10. A soil amendment according to claim 9 for promoting growth of microorganisms for achieving a combination of biological control and rational optimisation, wherein: the preparation method of the Metarrhizium anisopliae fermentation liquor comprises the following steps:
(1) activating metarhizium anisopliae: diluting conidia of Metarrhizium anisopliae by 5-10 times, coating on PDA solid culture medium, and culturing at 28 deg.C for 7 d to obtain activated Metarrhizium anisopliae;
(2) subculturing Metarrhizium anisopliae: selecting activated metarhizium anisopliae in a PDA solid culture medium by using an inoculating needle, and culturing at 28 ℃ for 7 d to obtain first-grade metarhizium anisopliae;
(3) and (3) fermentation culture of Metarrhizium anisopliae: and (3) beating the fungus block of the first-level metarhizium anisopliae in the step two into a PDB liquid culture medium, and culturing at 28 ℃ and 150 rpm for 56-72 h to obtain metarhizium anisopliae fermentation liquor.
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