CN114956342A - Method for restoring sulfadimidine polluted water body based on aquatic plant combination - Google Patents
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Abstract
The method for restoring the water body polluted by the sulfadimidine based on the aquatic plant combination comprises the steps of respectively exposing emergent aquatic plants and submerged plant seedlings to a sulfadimidine aqueous solution, continuously measuring SM2 concentration change, finally screening emergent aquatic plant recurrent flower and submerged plant tape grass crop restoration species with highest degradation efficiency, and pre-culturing; building a water tank, paving small stones at the bottom of the water tank to serve as a fixed matrix, fixing tape grass plants on the matrix at the bottom of the water tank, fixing the re-forcing flowers on a foam board and placing the re-forcing flowers and the tape grass in the water tank, and realizing the degradation of SM2 in the water body by exposing the re-forcing flowers and the tape grass in the SM2 polluted water body mode. The method comprehensively utilizes the water purification advantages of emergent aquatic plants and submerged plants, efficiently removes the antibiotic residues in the wastewater, has the degradation efficiency of SM2 reaching 61.47 percent, obviously reduces the Content of Oxygen Demand (COD), Total Nitrogen (TN) and Total Phosphorus (TP) in the wastewater, and has the advantages of simple operation method, wide application range, good applicability and environmental friendliness.
Description
Technical Field
The invention relates to a method for restoring sulfadimidine polluted water based on aquatic plant combination, belonging to the technical field of prevention and treatment of antibiotic polluted water.
Background
The antibiotic medicine is widely applied to livestock breeding and fish breeding industries in China, and can prevent and treat diseases of livestock and fish. However, more than 70% of applied antibiotics are discharged with the feces and urine into the environment. Antibiotic residual concentration between ng/L and mg/L can be detected in water and soil environment nationwide. The effluent and the reclaimed water of the urban sewage treatment plant are the first enrichment and distribution sources of environmental pollution factors, and the reclaimed water is ecologically supplemented for a long time, so that environmental and health risks are certainly generated. In particular, antibiotic concentrations in the regenerant water at ng/L to mg/L levels pose a significant threat to human health. However, conventional remediation techniques are not effective in removing such persistent drug contaminants. Therefore, it is imperative to develop new processes for efficient antibiotic removal.
Disclosure of Invention
Based on the background, the invention aims to construct a high-efficiency and easy-to-operate aquatic plant combination reactor and a wastewater treatment method, namely an antibiotic polluted water body remediation method based on aquatic plant combination and a reactor thereof, so that the stability of the traditional plant remediation technology is improved, meanwhile, an ideal process for efficiently removing inorganic nutrients and antibiotics in actual wastewater is realized, the dual targets of standard discharge of wastewater and reduction of environmental pollution are really realized, and a new platform for wastewater treatment of the next green revolution is developed.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
1. the method for restoring the sulfadimidine polluted water body based on the aquatic plant combination is characterized by comprising the following steps:
1) determination of dominant aquatic phytoremediation species
Respectively exposing emergent aquatic plants and submerged plant seedlings to a sulfadimidine aqueous solution, and continuously measuring the change of the concentration of the sulfadimidine in the aqueous solution, and finally screening emergent aquatic plant reliefs and submerged plant tape grass with the highest degradation efficiency as dominant aquatic plant repairing species;
2) preculture of dominant aquatic phytoremediation species
Cleaning the Thymus floribunda and the Sophora alopecuroides, and performing pre-culture respectively for the next step to construct an aquatic plant combined reactor;
3) construction of aquatic plant combination reactor
Building a water tank with the depth of 0.5-1.5m, paving small stones at the bottom of the water tank to be used as a fixed matrix, cutting leaves of the pre-cultured eel grass seedlings to 5 +/-1 cm, selecting 5 seedlings to form a cluster of eel grass plants, and controlling the biomass of the cluster of eel grass plants to be 10 +/-1 g; fixing the tape grass plants on the substrate at the bottom of the pool to make the number of plants per square meter not less than 200 plants/m 2 Injecting SM2 into the pool to pollute water body for at least 30cm and completely submerging the eel grass plants;
taking a foam board, digging a plurality of small holes on the foam board for fixing sponges according to the stem thickness of the respiring flower plants, and shearing a plurality of sponge strips with the width of 1cm and the length of 5 cm; selecting a re-stressed flower with the biomass of 10 +/-1 g, slightly winding a sponge strip at the position 1cm above the root of the re-stressed flower, and fixing the sponge strip at the hole digging position of a foam plate; placing the foam board with the immobilized Thalia dealbata plants in the water tank containing the SM2 polluted wastewater to ensure that the biomass per square meter is not lower than 2kg/m 2 And the roots of the Thalia dealbata plants are completely immersed in water;
4) repairing polluted water
Continuously injecting SM2 polluted water into the pool to a set water level, wherein the distance between the water level and the top of the pool is not more than 20 cm; the degradation of SM2 in the water body is realized by exposing the Thalia dealbata and the tape grass to the SM2 polluted water body;
monitoring the water quality by measuring the concentration of SM2 in the reactor wastewater every 2 days, and discharging the water in the water pool until the height of the water level is not lower than 30cm after the concentration of SM2 reaches a set mark; and re-injecting the SM2 contaminated water body for the next round of remediation.
The step 2) is to pre-culture the dominant aquatic phytoremediation species, and specifically comprises the following steps:
repeatedly cleaning the redroot seedlings with clear water until no soil is left at the roots; putting the cleaned redrawn seedlings into a container filled with distilled water, immersing the roots below the water surface, pre-culturing for 7-10 days, and replacing water every 2 days; the temperature is 30 +/-5 ℃ during the culture period, and the natural illumination condition is adopted;
removing impurities from the seedlings of the tape grass, repeatedly cleaning the seedlings by using tap water, fixing the roots by using clean small stones in a beaker as a root fixing matrix, slowly pouring distilled water into the beaker until the whole plant is immersed below the water surface, pre-culturing for 7-10 days, and replacing water every 2 days; the temperature during the culture period is 30 +/-5 ℃, and the natural illumination condition is adopted.
In the invention, the relish is known asThalia dealbata FraserPerennial emergent aquatic herbaceous plants of the arrowroot family and the taro genus. Is an emergent flower with extremely high ornamental value introduced into China. Besides being used for appreciation, the flowers also have the function of purifying water quality, and are usually planted in a water pool or a wetland in pieces, or potted plants for appreciation or planted in water landscape of a courtyard. The advantages of the Thalia dealbata on the repair of antibiotic-polluted wastewater are as follows:
1) stable and efficient removal efficiency;
2) the root system is especially developed, adventitious roots are densely distributed on the root stem, the length of the adventitious roots is 50-90 cm, lateral roots are arranged on the roots, and lateral roots on the upper layer are especially developed. The space of the underground roots and rhizomes of the Thalia dealbata is huge and is equivalent to that of the overground part.
In the invention, the tape grass is named asVallisneria natans (Lour.) HaraBianzimniao and Biancao. Is perennial non-stem submerged herb with stolons. Living in the environment of a brook, a river, etc. Widely distributed in freshwater of China. Has various values such as medical, ornamental, economic and the like. The eel grass has the following advantages for remedying the antibiotic-polluted wastewater:
1) the propagation speed of the tape grass is fast, and usually only one year is needed, one tape grass can form a length of 3m 2 Left and right clusters;
2) tape grass plays an important role in releasing oxygen, providing food and housing for animals, and in maintaining the health of aquatic ecosystems through the decomposition of organic pollutants.
Aiming at the problems of serious antibiotic pollution condition of urban wastewater, large limitation of the traditional restoration process, poor stability of a single plant bed restoration system and the like, the invention organically combines different types of aquatic plants, comprehensively utilizes different water body purification advantages of emergent aquatic plants and submerged aquatic plants, efficiently removes antibiotic residues in wastewater, and simultaneously reduces nutrient salts such as Chemical Oxygen Demand (COD), Total Nitrogen (TN), Total Phosphorus (TP) and the like in the wastewater, thereby achieving the purposes of no secondary pollution, water body purification and standard wastewater discharge.
The plant bed antibiotic degradation method adopted by the invention utilizes the plant root system to absorb and transfer pollutants so as to reduce the concentration of the antibiotic in the water body, the system is convenient to maintain, flexible to move and lower in construction and operation cost, and the plant biomass can be used for energy production, does not generate secondary pollution, and is a biological treatment technology for changing waste into valuable. Traditional plant bed systems are mostly limited to selecting single plant types, have limited degradation efficiency on antibiotics, and are not beneficial to the balance of a water body ecosystem after a long time.
In the invention, the degradation efficiency of the combined plant reactor of the religious flower and the tape grass to SM2 reaches 61.47 percent, which is obviously higher than that of a single plant reactor of the religious flower and the tape grass. Meanwhile, the combined plant reactor obviously reduces the Content of Oxygen Demand (COD), Total Nitrogen (TN) and Total Phosphorus (TP) in the wastewater. Therefore, the method has the advantages of strong practical applicability, simple operation method and wide application range, and simultaneously promotes the high efficiency, economy and environmental friendliness of the wastewater antibiotic pollution remediation work.
Drawings
FIG. 1 is a schematic diagram of a Reynaud flower + tape grass aquatic plant combination reactor of the present invention.
FIG. 2 is a graph of the power of the Reynaldia flower, tape grass single plant reactor and combined plant reactor of the present invention to remove SM2 from wastewater.
In the figure: 1. then, strengthening the flowers; 2. a sponge strip; 3. a foam board; 4. herba Swertiae Dilutae; 5. small stones.
Detailed Description
As shown in figure 1, the aquatic plant combined reactor for repairing sulfadimidine polluted water body related by the method comprises a water pool with the depth of 0.5-1.5m, a Thalictrum flower 1, a bitter grass 4, sea noodles 2, a foam board 3 and small pebbles 5; spreading small pebbles 5 as fixed matrix at the bottom of the pond, cutting the leaves of 4 seedling of the pre-cultured tape grass to 5 + -1 cm, and selecting5 seedlings are grouped into a cluster of tape grass plants, and the biomass of the cluster of tape grass plants is controlled to be 10 +/-1 g; fixing herba Swertiae Dilutae plants on the matrix at the bottom of the pool, wherein the number of plants per square meter is not less than 200 plants/m 2 ；
A plurality of small holes for fixing the sponge strips 2 are drilled in the foam plate 3, and a plurality of sponge strips 2 with the width of 1cm and the length of 5cm are cut; selecting a re-forcing flower 1 with biomass of 10 +/-1 g, slightly winding a sponge strip 2 at the position 1cm above the root of the re-forcing flower 1, and then fixing the re-forcing flower 1 at the hole digging position of a foam plate 3; placing the foam board 3 fixed with the Reli flower 1 plant in a pool containing SM2 polluted wastewater, wherein the biomass per square meter is not less than 2kg/m 2 The SM2 injected into the pool contaminated the water and fully submerged the tape grass plants, and then the roots of the tape grass plants were fully submerged in the water.
The invention specifically adopts the following steps:
1) determination of dominant aquatic phytoremediation species
1.1) selecting seedlings from existing emergent aquatic plants and submerged plants;
1.2) pre-culturing different aquatic plant seedlings in water for 7-10 days;
1.3) taking seedlings with the same biomass, exposing the seedlings to an antibiotic aqueous solution, measuring the concentration change of the antibiotic in an exposure solution every day, and predicting the antibiotic repair capacity of purchased aquatic plants;
1.4) selecting emergent aquatic plants and submerged plants with highest degradation efficiency as dominant restoration plants according to the measured antibiotic degradation efficiency data; finally, selecting emergent aquatic plants, religious flowers and submerged plants, tape grass as dominant repairing plants;
2) constructing a plant reactor:
2.1) collecting a water sample of effluent of a primary sedimentation tank of a sewage treatment plant, and determining the contents of Chemical Oxygen Demand (COD), Total Nitrogen (TN) and Total Phosphorus (TP) in the water sample;
2.2) constructing a re-forced flower plant reactor, a bitter grass plant reactor and a re-forced flower and bitter grass combined plant reactor with the same biomass after pre-mixed culture;
2.3) exposing 3 groups of reactors to wastewater of a sewage treatment plant containing 0.1mg/L sulfadimidine (SM 2) for a test period of 10 days;
2.4) measuring the concentration of SM2 in the wastewater of each group of plant reactors at intervals of 2 days;
2.5) determining the content of Chemical Oxygen Demand (COD), Total Nitrogen (TN) and Total Phosphorus (TP) in the wastewater on the 10 th day.
In the step 2.2), the pre-culture is specifically carried out in the following way:
repeatedly cleaning the redroot seedlings with clear water until no soil is left at the roots; putting the cleaned redroot seedlings into a beaker filled with tap water, immersing roots below the water surface, pre-culturing for 7-10 days, and replacing water every 2 days;
removing impurities from the seedlings of the tape grass, repeatedly cleaning the seedlings by using tap water, fixing the roots by using clean small stones as root fixing substrates, slowly pouring the tap water into a beaker until the whole plant is immersed below the water surface, pre-culturing for 7-10 days, and replacing the water every 2 days; the temperature during the culture period is 30 +/-5 ℃, and the natural illumination condition is adopted.
3) Dynamically detecting the antibiotic concentration of the wastewater and the Contents of Oxygen Demand (COD), Total Nitrogen (TN) and Total Phosphorus (TP)
And detecting the antibiotic concentration change of the wastewater in 3 groups of plant reactors 1 time every two days, and supplementing distilled water to the original liquid level height in time. 1ml of water sample to be tested is taken and filtered through a 0.45 mu m filter membrane. The concentration of antibiotics in the wastewater was determined using high performance liquid chromatography (HPLC, Alliance e2695 system, Waters, USA). The mobile phase ratio is 0.1% formic acid water solution, acetonitrile = 8: 2, ultraviolet detection wavelength 271nm, flow rate of 1ml/min and column temperature of 30 ℃.
In the step 2.2), a plant reactor is constructed as follows:
the bitter grass plant reactor: a pool is constructed, and a layer of small clean small stone fixing matrix with the size of about 2cm is paved at the bottom of the pool. Cutting the leaves of the pre-cultured tape grass seedlings to 5 +/-1 cm, selecting 5 seedlings as a cluster of tape grass plants, and controlling the biomass of the cluster of tape grass plants to be 10 +/-1 g. Fixing 12 clusters of tape grass plants at the bottom of the container, slowly pouring 8L of SM2 antibiotic-polluted wastewater of 1mg/L, completely immersing the tape grass plants, and exposing for 10 days.
Constructing a water pool with the same size as the bitter herb plant reactor, taking a plurality of rectangular foam plates, and digging a plurality of small holes on the foam plates for fixing the sponge according to the stem thickness of the bitter herb plant; cut several sponge strips with width of about 1cm and length of about 5 cm. Selecting a re-forcing flower with the biomass of 10 +/-1 g, lightly winding a sponge strip at the position 1cm above the root of the re-forcing flower, and then fixing the sponge strip at the hole digging position of a foam plate. Slowly pouring 8L of SM2 antibiotic polluted wastewater with the concentration of 1mg/L into a pool, placing the foam board on which the Reli flower plants are fixed into the SM2 antibiotic polluted wastewater with the concentration of 1mg/L with the concentration of 8L, and determining that the roots of the plants are completely immersed in the exposure liquid and exposed for 10 days.
The Relihua + tape grass plant reactor: constructing a water pool with the same size as the bitter grass plant reactor, and paving small pebbles to fix the substrate; cutting leaves of the pre-prepared and cultured tape grass seedlings to 5 +/-1 cm, selecting 5 seedlings as a cluster of tape grass plants, and controlling the biomass of the cluster of tape grass plants to be 10 +/-1 g; fixing 6 clusters of tape grass plants at the bottom of the container, slowly pouring 8L of SM2 antibiotic polluted wastewater of 1mg/L, and completely immersing the tape grass plants;
taking a plurality of rectangular foam boards as described above, digging a plurality of small holes on the foam boards for fixing sponges according to the stem thicknesses of the respirotic plants, and cutting a plurality of sponge strips with the width of 1cm and the length of 5 cm; selecting a religious flower with the biomass of about 10g, lightly winding a sponge strip at the position 1cm above the root of the religious flower, and fixing the sponge strip at the hole digging position of a foam plate; the foam plate with the replenisher plant fixed is placed in the SM2 antibiotic polluted wastewater with 1mg/L of the tape grass plant, and the root of the plant is determined to be completely immersed in the exposure liquid (shown in figure 1) and exposed for 10 days.
In the step 2.3), the method for preparing the antibiotic-polluted wastewater comprises the following steps:
about 30L of primary sedimentation tank effluent is collected at the primary sedimentation tank effluent position of a sewage treatment plant, and the collected wastewater is configured into 1mg/L of SM2 antibiotic polluted wastewater for standby application.
The results show that after 10 days, the removal rate of the antibiotics in the wastewater in the Reynaudian plus tape grass combined plant reactor reaches 61.47 percent, which is obviously higher than the removal efficiency of the antibiotics in the wastewater in a single kind of plant reactor (figure 2). The Content of Oxygen Demand (COD), Total Nitrogen (TN) and Total Phosphorus (TP) is determined by adopting a national standard method, and the result shows that the contents of COD, TN and TP are obviously reduced by the aid of the reserpine flower and tape grass combined plant reactor.
The above description is only an embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, improvement, extension, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.
Claims (3)
1. The method for restoring the water body polluted by the sulfadimidine based on the aquatic plant combination is characterized by comprising the following steps:
1) determination of dominant aquatic phytoremediation species
Respectively exposing emergent aquatic plants and submerged plant seedlings to a sulfadimidine aqueous solution, and continuously measuring the change of the concentration of the sulfadimidine in the aqueous solution, and finally screening emergent aquatic plant reliefs and submerged plant tape grass with the highest degradation efficiency as dominant aquatic plant repairing species;
2) preculture of dominant aquatic phytoremediation species
Cleaning the Thymus floribunda and the Sophora alopecuroides, and performing pre-culture respectively for the next step to construct an aquatic plant combined reactor;
3) construction of aquatic plant combination reactor
Building a water tank with the depth of 0.5-1.5m, paving small stones at the bottom of the water tank to be used as a fixed matrix, cutting leaves of the pre-cultured eel grass seedlings to 5 +/-1 cm, selecting 5 seedlings to form a cluster of eel grass plants, and controlling the biomass of the cluster of eel grass plants to be 10 +/-1 g; fixing tape grass plants on the substrate at the bottom of the pool to make the number of plants per square meter not less than 200 plants/m 2 Injecting SM2 into the pool to pollute water body for at least 30cm and completely submerging the eel grass plants;
taking a foam board, digging a plurality of small holes on the foam board for fixing sponges according to the stem thickness of the respiring flower plants, and shearing a plurality of sponge strips with the width of 1cm and the length of 5 cm; selecting Zanthoxylum schinifolium with biomass of 10 + -1 g, and culturingThe sponge strip is gently wound at the position 1cm above the root of the resurgent flower and then fixed at the hole digging position of the foam board; placing the foam board with the immobilized Thalia dealbata plants in the water tank containing the SM2 polluted wastewater to ensure that the biomass per square meter is not lower than 2kg/m 2 And the roots of the Thalia dealbata plants are completely immersed in water;
4) repairing polluted water
Continuously injecting SM2 polluted water into the pool to a set water level, wherein the distance between the water level and the top of the pool is not more than 20 cm; the degradation of SM2 in the water body is realized by exposing the Thalia dealbata and the tape grass to the SM2 polluted water body;
monitoring the water quality by measuring the concentration of SM2 in the reactor wastewater every 2 days, and discharging the water in the water pool until the height of the water level is not lower than 30cm after the concentration of SM2 reaches a set mark; and re-injecting the SM2 contaminated water body for the next round of remediation.
2. The method for remediating a water body polluted by sulfadimidine based on aquatic plant combination as claimed in claim 1, wherein the step 2) is to pre-culture the dominant aquatic plant remediation species as follows:
repeatedly cleaning the redroot seedlings with clear water until no soil is left at the roots; putting the cleaned redrawn seedlings into a container filled with distilled water, immersing the roots below the water surface, pre-culturing for 7-10 days, and replacing water every 2 days; the temperature is 30 +/-5 ℃ during the culture period, and the natural illumination condition is adopted;
removing impurities from tape grass seedlings, repeatedly cleaning with tap water, fixing roots by using clean cobblestones in a beaker as a root fixing matrix, slowly pouring distilled water into the beaker until the whole plant is immersed below the water surface, pre-culturing for 7-10 days, and replacing water every 2 days; the temperature during the culture period is 30 +/-5 ℃, and the natural illumination condition is adopted.
3. An aquatic plant combined reactor for repairing sulfadimidine polluted water body is characterized by comprising a water pool with the depth of 0.5-1.5m, religious flower (1), eel grass (4), sea noodle (2) and foamThe plate (3) is small stones (5); small stones (5) serving as a fixed matrix are paved at the bottom of the pool, seedling leaves of the pre-prepared tape grass (4) are cut to 5 +/-1 cm, 5 seedlings are selected to form a tape grass plant, and the biomass of the tape grass plant is controlled to be 10 +/-1 g; fixing herba Swertiae Dilutae plants on the matrix at the bottom of the pool, wherein the number of plants per square meter is not less than 200 plants/m 2 ；
A plurality of small holes for fixing the sponge (2) are drilled in the foam plate (3), and a plurality of sponge strips (2) with the width of 1cm and the length of 5cm are cut; selecting a re-forcing flower (1) with the biomass of 10 +/-1 g, slightly winding a sponge strip (2) at the position 1cm above the root of the re-forcing flower (1), and then fixing the re-forcing flower (1) at the hole digging position of a foam plate (3); placing the foam board (3) with the immobilized Thalia dealbata (1) plant in a pool containing SM2 polluted wastewater, wherein the biomass per square meter is not less than 2kg/m 2 The SM2 injected into the pool contaminated the water and fully submerged the tape grass plants, and then the roots of the tape grass plants were fully submerged in the water.
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| CN116605995A (en) * | 2023-03-31 | 2023-08-18 | 北京首创生态环保集团股份有限公司 | Method for degrading sulfonamide antibiotics by using constructed wetland |
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