CN114949040A - Total flavonoids of nymphaea hybrid, and extraction method and application thereof - Google Patents
Total flavonoids of nymphaea hybrid, and extraction method and application thereof Download PDFInfo
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- CN114949040A CN114949040A CN202210179538.8A CN202210179538A CN114949040A CN 114949040 A CN114949040 A CN 114949040A CN 202210179538 A CN202210179538 A CN 202210179538A CN 114949040 A CN114949040 A CN 114949040A
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- nymphaea hybrid
- nymphaea
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Abstract
The invention discloses a nymphaea hybrid total flavone as well as an extraction method and application thereof, belonging to the technical field of plant extraction. The extraction method of the invention comprises the following steps: (1) adding cellulase and ethanol into the nymphaea hybrid powder, performing ultrasonic extraction, filtering, and taking supernatant to obtain a crude extract of nymphaea hybrid total flavonoids; (2) regulating the concentration and pH of the crude extract of the nymphaea hybrid general flavone to obtain a sample liquid, loading the sample liquid on a macroporous resin adsorption column, eluting, and collecting the eluent to obtain the nymphaea hybrid general flavone; the mass ratio of the nymphaea hybrid powder to the cellulase is 1: 0.01-0.05; the mass volume ratio of the nymphaea hybrid powder to the ethanol is 1g: 20-60 mL. The method can extract the total flavonoids of the nymphaea hybrid, has high yield, can improve the antioxidant and anti-inflammatory activities of the total flavonoids after purifying the total flavonoids, can be added into antioxidant and anti-inflammatory cosmetics or foods, and has good application prospect.
Description
Technical Field
The invention relates to a nymphaea hybrid total flavone, and an extraction method and application thereof, and belongs to the technical field of natural product extraction.
Background
Nymphaea hybrid is a herbal plant of hydrophytic perennial root of Nymphaeaceae (Nymphaeaceae) Nymphaeaceae, has aromatic flavor, bright color and large flower, and is a good ornamental plant. The main active ingredients of the nymphaea hybrid comprise flavonoids, phenolic acids, alkaloids, lignans, polysaccharides and other substances, and the nymphaea hybrid has the effects of resisting oxidation, resisting bacteria, resisting inflammation, resisting radiation, reducing blood sugar and blood pressure, and has wide application prospects when being applied to industries such as functional foods, medicines, cosmetics and the like. In the prior art, when the nymphaea hybrid general flavone is extracted, the time is long, the extraction rate is low, the pharmacological effect of the obtained nymphaea hybrid general flavone is not obvious, and an extraction method which has high extraction efficiency and better antioxidant and anti-inflammatory effects is not found.
Disclosure of Invention
The technical problem solved by the invention is as follows: the prior art has the problems of long time consumption, low extraction rate, unobvious pharmacological action of the obtained nymphaea hybrid general flavone and the like when the nymphaea hybrid general flavone is extracted.
In order to solve the technical problems, the invention provides a method for extracting total flavonoids in nymphaea hybrid, which comprises the following steps:
step 1: mixing the nymphaea hybrid pollen, the cellulase and the ethanol, carrying out ultrasonic extraction, filtering, and taking the supernatant to obtain a crude extract of the nymphaea hybrid total flavonoids; wherein the mass ratio of the nymphaea hybrid pollen to the cellulase is 1: 0.01-0.05; the mass volume ratio of the nymphaea hybrid powder to the ethanol is 1g: 20-60 mL;
step 2: adjusting the concentration of the obtained crude extract of the nymphaea hybrid general flavone to 0.35-1.72 mg/mL, adjusting the pH to 2-6 to obtain a sample liquid, loading the sample liquid on a macroporous resin adsorption column, eluting, and collecting the eluate to obtain the nymphaea hybrid general flavone.
Preferably, the nymphaea hybrid powder in the step 1 is the nymphaea hybrid powder collected after being sieved by a sieve of 40-60 meshes; the concentration of the ethanol is 40-60 vt%.
Preferably, the power of ultrasonic extraction in the step 1 is 140-160W, the temperature is 45-55 ℃, and the time is 25-35 min.
Preferably, the filtration in step 1 is performed by suction filtration through a buchner funnel.
Preferably, the solvent used for adjusting the concentration of the crude extract of the total flavonoids in the nymphaea hybrid in the step 2 is distilled water; the reagent for adjusting the pH value of the crude extraction liquid of the nymphaea hybrid general flavone is dilute hydrochloric acid.
Preferably, the model of the macroporous resin filled in the macroporous resin adsorption column in the step 2 is D-101, AB-8, HP-20 or NKA-9.
Preferably, the mass volume ratio of the macroporous resin in the macroporous resin adsorption column to the sample loading liquid in the sample loading in the step 2 is 1g: 14-24 mL; the flow rate of the sample liquid is 0.5-3 mL/min.
Preferably, the solvent eluted in the step 2 is ethanol; the concentration of the ethanol is 50-90 vt%; the dosage of the ethanol is 0.5-1.5 times of the volume of the sample loading liquid; the flow rate of elution is 0.5-3 mL/min.
The invention also provides the nymphaea hybrid general flavone extracted by the extraction method of the nymphaea hybrid general flavone.
The invention also provides application of the nymphaea hybrid total flavonoids in preparation of cosmetics or foods with oxidation resistance and/or anti-inflammation.
Compared with the prior art, the invention has the following beneficial effects:
1. the method combines ultrasonic extraction and enzymolysis extraction to extract the total flavonoids in the nymphaea hybrid, and has the advantages of high efficiency, high extraction rate, greenness, safety and sustainability;
2. the nymphaea hybrid general flavone obtained by the extraction method disclosed by the invention is high in yield, and the extracted nymphaea hybrid general flavone is high in physiological activity after being purified, can effectively remove DPPH free radicals and ABTS free radicals, has an obvious inhibition effect on hyaluronidase, can be added into antioxidant and anti-inflammatory cosmetics or foods, and has a good application prospect.
Drawings
FIG. 1 shows the DPPH free radical scavenging ability of total flavones of nymphaea hybrid; wherein NHE represents the crude extraction liquid of unpurified nymphaea hybrid total flavonoids, NHEP represents the purified nymphaea hybrid total flavonoids, and Vc represents the scavenging capacity of vitamin C on DPPH free radicals;
FIG. 2 shows the scavenging ability of total flavonoids of nymphaea hybrid to ABTS free radicals; wherein NHE represents the crude extraction liquid of unpurified nymphaea hybrid total flavonoids, NHEP represents the purified nymphaea hybrid total flavonoids, and Vc represents the scavenging capacity of vitamin C on ABTS free radicals;
FIG. 3 shows the hyaluronidase inhibition rate of total flavones of nymphaea hybrid; wherein NHE represents the crude extraction liquid of unpurified nymphaea hybrid total flavonoids, NHEP represents the purified nymphaea hybrid total flavonoids, and Dg represents the inhibition capacity of dipotassium glycyrrhizinate on transparent phytase.
Detailed Description
In order to make the invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.
The invention provides an extraction method of total flavonoids in nymphaea hybrid, which comprises the following steps:
(1) mixing the nymphaea hybrid pollen, the cellulase and the ethanol, carrying out ultrasonic extraction, filtering, and taking the supernatant to obtain a crude extract of the nymphaea hybrid total flavonoids;
(2) adjusting the concentration and pH of the crude extract of the nymphaea hybrid general flavone to obtain a sample liquid, loading the sample liquid on a macroporous resin adsorption column, eluting, and collecting eluent to obtain the nymphaea hybrid general flavone;
the mass ratio of the nymphaea hybrid powder to the cellulase is 1: 0.01-0.05;
the mass volume ratio of the nymphaea hybrid powder to the ethanol is 1g: 20-60 mL;
adjusting the concentration of the crude extract of the total flavonoids of the nymphaea hybrid to 0.35-1.72 mg/mL;
and adjusting the pH value of the crude extraction liquid of the nymphaea hybrid general flavone to 2-6.
In the invention, the nymphaea hybrid powder is collected after being sieved by a sieve of 40-60 meshes; the mesh number of the screening is preferably 50 meshes.
In the invention, the preparation method of the nymphaea hybrid powder comprises the following steps: drying, crushing and sieving the nymphaea hybrid to obtain the nymphaea hybrid powder.
In the present invention, the drying is preferably performed by freeze-drying in a freeze dryer, and the pulverization is performed by a 50-mesh sieve.
In the invention, the concentration of the ethanol is 40-60 vt%, preferably 50 vt%;
the mass-volume ratio of the nymphaea hybrid powder to the ethanol is preferably 1 g/30-50 mL, and more preferably 1 g/40 mL.
In the invention, the mass ratio of the nymphaea hybrid powder to the cellulase is preferably 1: 0.02-0.04, and further preferably 1: 0.03.
In the invention, the power of ultrasonic extraction is 140-160W, preferably 150W; the ultrasonic extraction temperature is 45-55 ℃, and preferably 50 ℃; the ultrasonic extraction time is 25-35 min, preferably 30 min.
The filtering mode of the invention is suction filtration through a Buchner funnel.
In the invention, the solvent used for adjusting the concentration of the crude extraction liquid of the total flavonoids in the nymphaea hybrid is distilled water;
the reagent for adjusting the pH value of the crude extraction liquid of the nymphaea hybrid general flavone is dilute hydrochloric acid.
In the invention, the concentration and the pH value of the nymphaea hybrid general flavone in the sample liquid are adjusted to increase the adsorption capacity of the macroporous resin to the nymphaea hybrid general flavone.
In the invention, the macroporous resin filled in the macroporous resin adsorption column is D-101, AB-8, HP-20 or NKA-9 in type.
In the invention, the mass-volume ratio of the macroporous resin in the macroporous resin adsorption column to the sample loading liquid during sample loading is 1g: 14-24 mL, preferably 1g:19 mL;
the flow rate of the sample liquid is 0.5-3 mL/min, preferably 1-2.5 mL/min, and more preferably 1.75 mL/min.
In the present invention, the eluting solvent is ethanol;
the concentration of the ethanol is 50-90 vt%, preferably 60-80 vt%, and further preferably 70 vt%;
the dosage of the ethanol is 0.5-1.5 times of the volume of the sample loading liquid;
the flow rate of elution is 0.5-3 mL/min.
The invention also provides the total flavonoids of the nymphaea hybrid extracted by the extraction method.
The invention also provides application of the nymphaea hybrid general flavone in preparation of cosmetics or foods with antioxidant and/or anti-inflammatory effects.
The method for determining the total flavonoids of the nymphaea hybrid in the following examples and application examples comprises the following steps of: detecting the content of the total flavone by an aluminum trichloride chromogenic method. The absorbance at 425nm was measured using 75 vt% ethanol as a blank.
The standard substance is a rutin standard substance, and a standard regression equation obtained according to the absorbance (Y) and the concentration (X) is as follows: y is 0.0078X-0.0008, R 2 ＝0.9992。
The content of the total flavone is the mass (mg) of the total flavone/the mass (g) of the nymphaea hybrid powder.
Example 1
Drying and crushing the nymphaea hybrid, sieving the nymphaea hybrid with a 50-mesh pharmacopeia sieve, and collecting the components below the sieve to obtain the nymphaea hybrid powder.
Accurately weighing 1g of nymphaea hybrid pollen and 0.01g of cellulase, adding the nymphaea hybrid pollen and the cellulase into 40mL of 40 vt% ethanol, performing ultrasonic extraction for 25min under the conditions that the ultrasonic power is 140W and the ultrasonic temperature is 50 ℃, performing suction filtration by using a Buchner funnel, taking supernatant to obtain a nymphaea hybrid total flavone crude extract, and determining the content of total flavonoids in the nymphaea hybrid total flavone crude extract.
The content of the total flavonoids in the crude extract of the total flavonoids in the nymphaea hybrid obtained in the embodiment is 26.4 mg/g.
Example 2
Drying and crushing the nymphaea hybrid, sieving the nymphaea hybrid with a 40-mesh pharmacopeia sieve, and collecting the components below the sieve to obtain the nymphaea hybrid powder.
Accurately weighing 1g of nymphaea hybrid pollen and 0.05g of cellulase, adding the nymphaea hybrid pollen and the cellulase into 20mL of 60 vt% ethanol, performing ultrasonic extraction for 35min under the conditions that the ultrasonic power is 160W and the ultrasonic temperature is 45 ℃, performing suction filtration by using a Buchner funnel, taking supernatant to obtain a nymphaea hybrid total flavone crude extract, and determining the content of total flavonoids in the nymphaea hybrid total flavone crude extract.
The content of the total flavonoids in the crude extract of the total flavonoids in the nymphaea hybrid obtained in the embodiment is 27.06 mg/g.
Example 3
Drying and crushing the nymphaea hybrid, sieving the nymphaea hybrid with a 50-mesh pharmacopeia sieve, and collecting the components below the sieve to obtain the nymphaea hybrid powder.
Accurately weighing 1g of nymphaea hybrid pollen and 0.03g of cellulase, adding the nymphaea hybrid pollen and the cellulase into 60mL of 50 vt% ethanol, performing ultrasonic extraction for 30min under the conditions that the ultrasonic power is 150W and the ultrasonic temperature is 55 ℃, performing suction filtration by using a Buchner funnel, taking supernatant to obtain a nymphaea hybrid total flavone crude extract, and determining the content of total flavonoids in the nymphaea hybrid total flavone crude extract.
The content of the total flavonoids in the crude extract of the total flavonoids in the nymphaea hybrid obtained in the embodiment is 26.62 mg/g.
Example 4
Weighing 5g D-101 macroporous resin, and loading in glass packed column to obtain D-101 macroporous resin adsorption column. The concentration of the total flavonoids of the nymphaea hybrid obtained in example 1 was adjusted to 1.72mg/mL with distilled water to obtain an intermediate solution, and the pH of the intermediate solution was adjusted to 6.0 with diluted hydrochloric acid to obtain a loading solution. And (3) sampling 120mL of the sample liquid into a D-101 macroporous resin adsorption column, adjusting the flow rate of the sample to be 3mL/min, and after the sample is completely sampled, performing elution treatment by using 90 vt% ethanol, wherein the using amount of the ethanol is 120mL, and the flow rate of the elution is 3 mL/min. Collecting eluent after the elution is finished to obtain the nymphaea hybrid general flavone.
Example 5
And 5g of AB-8 macroporous resin is weighed and filled in a glass packed column to obtain an AB-8 macroporous resin adsorption column for later use.
The concentration of the total flavonoids of the nymphaea hybrid obtained in example 2 was adjusted to 0.35mg/mL with distilled water to obtain an intermediate solution, and the pH of the intermediate solution was adjusted to 2.0 with diluted hydrochloric acid to obtain a loading solution. And (3) sampling 100mL of the sample liquid in an AB-8 macroporous resin adsorption column, adjusting the flow rate of the sample to be 1mL/min, and after the sample is completely sampled, performing elution treatment by using 50 vt% ethanol, wherein the using amount of the ethanol is 50mL, and the flow rate of the elution is 1 mL/min. Collecting eluent after the elution is finished to obtain the nymphaea hybrid general flavone.
Example 6
And weighing 5g of NKA-9 macroporous resin, and filling the NKA-9 macroporous resin into a glass filled column to obtain the NKA-9 macroporous resin adsorption column for later use.
The concentration of the total flavonoids of the nymphaea hybrid obtained in example 3 was adjusted to 0.8mg/mL with distilled water to obtain an intermediate solution, and the pH of the intermediate solution was adjusted to 4.0 with diluted hydrochloric acid to obtain a sample solution. And (3) sampling 70mL of the sample solution into an NKA-9 macroporous resin adsorption column, adjusting the flow rate of the sample to be 0.5mL/min, and after the sample is completely sampled, performing elution treatment by using 60 vt% ethanol, wherein the using amount of the ethanol is 105mL, and the flow rate of the elution is 0.5 mL/min. And collecting the eluent after the elution is finished to obtain the nymphaea hybrid general flavone.
Application example 1 measurement of DPPH radical scavenging ability
Preparing the crude extraction solution of the total flavonoids of the nymphaea hybrid obtained in the example 1 and the total flavonoids of the nymphaea hybrid obtained in the example 4 into solutions with different concentrations, namely, the solutions with the concentrations of 1 mu g/mL, 5 mu g/mL, 10 mu g/mL, 15 mu g/mL, 20 mu g/mL, 25 mu g/mL, 30 mu g/mL and 60 mu g/mL, respectively mixing 2mL of the solutions with different concentrations with 2mL of DPPH solution with the concentration of 0.1mmol/L to obtain sample solutions with different concentrations, sealing the sample solutions with a preservative film, and carrying out dark reaction for 30min at the temperature of 25 ℃. The absorbance value was measured at 517nm and recorded as A. Mixing 2mL of absolute ethyl alcohol and 2mL of distilled water as a blank group for zero setting; taking a solution obtained by adding 2mL of sample solution into 2mL of absolute ethyl alcohol and reacting for 30min as a blank control group, wherein the absorbance value measured at 517nm is Ai, and the blank control group is used for removing the absorbance existing in the total flavone sample; a model control of 2mL of 0.1mmol/LDPPH solution plus 2mL of absolute ethanol was used, and the absorbance at 517nm was recorded as A0. The concentration is used as an abscissa and the clearance is used as an ordinate to draw a clearance effect graph, and the clearance of Vc to DPPH is used as a contrast. The results are shown in FIG. 1.
The solvent used for preparing the DPPH solution is absolute ethyl alcohol.
The clearance calculation formula is:
DPPH clearance ═ 1- (Ai-a)/a0 × 100%;
a is a light absorption value measured by adding 2mL of DPPH into 2mL of sample solution;
ai is a light absorption value measured by adding 2mL of absolute ethyl alcohol into 2mL of sample solution;
a0 is the absorbance measured for 2mL of DPPH solution with 2mL of absolute ethanol.
Fig. 1 shows that the crude extraction liquid of the total flavonoids of the nymphaea hybrid and the nymphaea hybrid have certain capacity of removing DPPH free radicals, but the removal effect of the purified nymphaea hybrid on the DPPH free radicals is better than that of the crude extraction liquid of the total flavonoids of the nymphaea hybrid which is not purified. The purification method of the invention has better purification effect on the total flavonoids in the nymphaea hybrid.
Application example 2 determination of ABTS radical scavenging ability
Mixing an ABTS solution with the concentration of 7mmol/L and a potassium persulfate solution with the concentration of 2.45mmol/L according to the volume ratio of 1:1 to prepare a cation working solution of an ABTS free radical, and keeping out of the sun for 12-14 h to excite the ABTS free radical. ABTS free radicals are diluted by PBS until the light absorption value is 0.7 +/-0.02 to prepare ABTS working solution.
Respectively preparing the crude extraction solution of the total flavonoids of the nymphaea hybrid obtained in the example 2 and the total flavonoids of the nymphaea hybrid obtained in the example 5 into solutions with different concentrations, namely solutions with the concentrations of 1 mu g/mL, 10 mu g/mL, 20 mu g/mL, 30 mu g/mL and 50 mu g/mL, respectively taking 0.09mL of the solution with each concentration, respectively adding 0.11mLABTS working solution, uniformly mixing, keeping out of the sun, reacting at 37 ℃ for 10min, and measuring the light absorption value A at 734 nm; respectively taking 0.09mL of each concentration solution, respectively adding 0.11mL of PBS solution, reacting for 10min at 37 ℃ in a dark place, and measuring a light absorption value Ai at 734 nm; then 0.11mL of the working solution of LABTS was reacted with 0.09mL of deionized water, and the absorbance A0 at 734nm was measured as a reference. The results are shown in FIG. 2, comparing the effect of Vc on ABTS clearance.
The calculation formula is as follows:
ABTS clearance ═ 1- (Ai-a)/a0] × 100%;
a is the light absorption value of 0.09mL of sample solution added with 0.11mL of ABTS working solution;
ai is the light absorption value of 0.09mL of sample solution added with 0.11mL of PBS;
a0 Absorbance of 0.11mL ABTS was added to 0.09mL deionized water.
Fig. 2 shows that the crude extraction liquid of the total flavonoids of the nymphaea hybrid and the nymphaea hybrid have certain capability of removing ABTS free radicals, but the removal effect of the purified nymphaea hybrid on the ABTS free radicals is better than that of the crude extraction liquid of the total flavonoids of the nymphaea hybrid which is not purified.
Application example 3 measurement of hyaluronidase inhibition
(1) Taking 0.1mL of 0.25mmol/L calcium chloride solution and 0.5mL of hyaluronidase solution, and carrying out incubation at 37 ℃ for 20 min;
(2) adding the crude extract of the total flavonoids of the nymphaea hybrid obtained in example 3 and 0.5mL of the total flavonoids of the nymphaea hybrid obtained in example 6 at different concentrations, and continuously culturing at 37 ℃ for 20 min;
(3) adding 0.5mL sodium hyaluronate solution, incubating at 37 deg.C for 30min, and standing at 25 deg.C for 5 min;
(4) adding 0.1mL of 0.4mol/L sodium hydroxide solution and 0.5mL of acetylacetone solution, heating in water at 100 ℃ for 15min, and immediately cooling with ice water for 5 min;
(5) adding 1.0mL of an Ellisib reagent, diluting with 3.0mL of absolute ethyl alcohol, standing at 25 ℃ for 20min for color development, and measuring the light absorption value of 585nm by using a spectrophotometer to obtain the light absorption value of a sample group.
The different concentrations are 0.16mg/mL, 0.32mg/mL, 0.62mg/mL, 1.25mg/mL, 2.5mg/mL, and 5mg/mL solutions.
And (3) carrying out reaction by using an acetic acid buffer solution with the concentration of 0.1mol/L and the pH value of 3.5 instead of the total flavonoid solution of the nymphaea hybrid, and taking the obtained light absorption value as a blank group to obtain the light absorption value of the blank group.
The reaction was carried out using an acetate buffer solution having a concentration of 0.1mol/L and a pH of 3.5 in place of hyaluronidase, and the absorbance obtained was used as the absorbance of the sample control.
The absorbance of the blank was measured using an acetate buffer solution at a concentration of 0.1mol/L pH 3.5.
The inhibitory effect of dipotassium glycyrrhizinate on hyaline phytase was used as a control and the results are shown in FIG. 3.
The hyaluronidase inhibition (%) is [ (A-B) - (C-D) ]/(A-B) × 100%,
a is the light absorption value of the blank group;
b is the light absorption value of the blank control group;
c is the light absorption value of the sample group;
d is the absorbance of the sample control group.
Fig. 3 shows that the crude extract of the total flavonoids of the nymphaea hybrid and the nymphaea hybrid can inhibit hyaluronidase, but the inhibition effect of the purified total flavonoids of the nymphaea hybrid is superior to that of the crude extract of the unpurified total flavonoids of the nymphaea hybrid.
While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (10)
1. A method for extracting total flavonoids in nymphaea hybrid is characterized by comprising the following steps:
step 1: mixing the nymphaea hybrid pollen, the cellulase and the ethanol, carrying out ultrasonic extraction, filtering, and taking the supernatant to obtain a crude extract of the nymphaea hybrid total flavonoids; wherein the mass ratio of the nymphaea hybrid powder to the cellulase is 1: 0.01-0.05; the mass volume ratio of the nymphaea hybrid powder to the ethanol is 1g: 20-60 mL;
step 2: adjusting the concentration of the obtained crude extract of the nymphaea hybrid general flavone to 0.35-1.72 mg/mL, adjusting the pH to 2-6 to obtain a sample liquid, loading the sample liquid on a macroporous resin adsorption column, eluting, and collecting the eluent to obtain the nymphaea hybrid general flavone.
2. The method for extracting the nymphaea hybrid general flavones according to claim 1, wherein the nymphaea hybrid pollen in the step 1 is the nymphaea hybrid pollen collected after being sieved by a sieve of 40-60 meshes; the concentration of the ethanol is 40-60 vt%.
3. The extraction method of total flavones of nymphaea hybrid according to claim 2, wherein the ultrasonic extraction power in the step 1 is 140-160W, the temperature is 45-55 ℃, and the time is 25-35 min.
4. The method for extracting total flavonoids from nymphaea hybrid according to claim 3, wherein the filtration in step 1 is performed by suction filtration through a Buchner funnel.
5. The extraction method of the total flavonoids in the nymphaea hybrid according to claim 4, wherein the solvent used for adjusting the concentration of the crude extract of the total flavonoids in the nymphaea hybrid in the step 2 is distilled water; the reagent for adjusting the pH value of the crude extraction liquid of the nymphaea hybrid general flavone is dilute hydrochloric acid.
6. The method for extracting total flavonoids in nymphaea hybrid according to claim 5, wherein the macroporous resin filled in the macroporous resin adsorption column in the step 2 is D-101, AB-8, HP-20 or NKA-9 in type.
7. The extraction method of the total flavonoids in the nymphaea hybrid according to claim 6, wherein the mass volume ratio of the macroporous resin in the macroporous resin adsorption column to the sample loading liquid in the step 2 is 1g: 14-24 mL; the flow rate of the sample liquid is 0.5-3 mL/min.
8. The method for extracting total flavonoids from nymphaea hybrid according to claim 7, wherein the solvent eluted in the step 2 is ethanol; the concentration of the ethanol is 50-90 vt%; the dosage of the ethanol is 0.5-1.5 times of the volume of the sample loading liquid; the flow rate of elution is 0.5-3 mL/min.
9. The nymphaea hybrid general flavone extracted by the extraction method of the nymphaea hybrid general flavone according to any one of claims 1 to 8.
10. Use of total flavonoids of nymphaea hybrid according to claim 9 for the preparation of cosmetics or foodstuffs with antioxidant and/or anti-inflammatory properties.
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| CN105106327A (en) * | 2015-09-10 | 2015-12-02 | 广西大学 | Process for extracting total flavones of red-knees herb by adopting enzymolysis-ultrasonic coupling method |
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| CN105106327A (en) * | 2015-09-10 | 2015-12-02 | 广西大学 | Process for extracting total flavones of red-knees herb by adopting enzymolysis-ultrasonic coupling method |
| CN107412431A (en) * | 2017-07-21 | 2017-12-01 | 锦州医科大学 | Ultrasonic assistant extracts Physalis pubescens L general flavone and purifying process |
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