CN114933978A - Arbuscular mycorrhizal fungi culture medium - Google Patents
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Abstract
The invention provides an arbuscular mycorrhizal fungi culture medium, and belongs to the field of microbial culture. The culture medium comprises corn straw biochar, bentonite, zinc sulfite, magnesium sulfate, sodium alginate, vitamin B2, glutathione, glycylglycine, lignin, glucose and fructose, and the arbuscular mycorrhizal fungi are cultured by the culture medium and are co-cultured with plants, so that the plant root system and microbial environment can be effectively improved, the heavy metal tolerance of the plants is improved, and the culture medium has important significance for agricultural sustainable development and ecological environment protection.
Description
Technical Field
The invention belongs to the field of microbial culture, and particularly relates to an arbuscular mycorrhizal fungi culture medium.
Background
Arbuscular Mycorrhizal Fungi (AMF) are soil fungi abundantly present in nature that are capable of infecting plant roots and forming mycorrhizal symbionts with plant roots. Arbuscular mycorrhiza formed by symbiosis of AMF hypha and a host plant nutrient root system is a representative of a plant-fungus reciprocal symbiotic system widely distributed in an ecosystem, and plays an important role in formation of plant community productivity and maintenance of biological diversity. Research has shown that about 80% of terrestrial plants worldwide are able to form symbiotic systems with AMF, and some plants are highly dependent on AMF. In a symbiotic system formed by AMF and a host root system, one end of AMF hypha is invaded into epidermal cells of the host root system to form a arbuscular structure, inorganic compounds (nitrogen and phosphate) are used for exchanging with carbohydrates and fatty acids of the host cells and secreting mycorrhizal factors to activate the expression of host related genes, and the other end of AMF hypha forms extra-root hypha to enlarge the range of the host root system for absorbing nutrients. The AMF obtains carbon-containing organic matters from a host body to meet the growth and propagation of the AMF, and researches show that about 20 percent of photosynthetic products in the host body are used for the consumption of the AMF, one part of the photosynthetic products is used for the growth and metabolism of the AMF, and the other part of the photosynthetic products is released into the soil in the form of small molecular organic compounds to induce rhizosphere bacteria to colonize nearby or on the surface of hyphae, so that the soil microbial community structure is improved, and the rhizosphere microenvironment of host plants is further changed. The symbiosis of the AMF can provide more nutrients and moisture for the root system of the host, and the absorption and utilization efficiency of the nutrients by the host is increased. In addition, AMF can also regulate the synthesis and distribution of hormones in host plants by influencing relevant metabolic pathways and gene expression in the host plants and the like, promote the growth and development of the host plants, and play an important role in regulating and controlling the growth and propagation of the plants due to the reciprocal and mutualistic symbiosis relationship formed by the two in the long-term co-evolution process.
The existing culture method of arbuscular mycorrhizal fungi comprises a pot culture method, a culture medium culture method, a nutrient solution culture method, a steam fog culture method, a glass bead chamber culture method, an in vitro sterile pure culture method and the like, and most of the culture processes have high requirements on culture mediums, so that the culture cost is increased. For example, patent CN201811102296.2 discloses an arbuscular mycorrhizal fungi culture medium, which comprises modified bentonite ceramic sand, and a modification solution for modifying the bentonite ceramic sand comprises: ferrous sulfate, manganese sulfate, copper nitrate, zinc nitrate, vitamin B2, vitamin B3, vitamin B6, sucrose, glucose, lysine, disodium ethylene diamine tetraacetate, sodium molybdate and cobalt chloride, wherein the modification liquid is adopted to carry out ultrasonic oscillation on the bentonite ceramic sand under low pressure, and the conditions are harsh. Patent CN200910098981.7 discloses a synthetic culture medium of arbuscular mycorrhizal fungi, said culture medium comprising: the cotton bud is selected from magnesium sulfate, potassium nitrate, potassium chloride, calcium nitrate, ferric sodium EDTA, inositol, manganese sulfate, copper sulfate, boric acid, zinc sulfate, potassium iodide, sodium molybdate, cobalt chloride, vitamin B, pyridoxine, nicotinic acid, sucrose, glucose and plant gum, etc., and the culture medium is adopted to culture arbuscular mycorrhizal fungi with slow propagation speed. Therefore, the further optimization of the culture medium of the arbuscular mycorrhizal fungi is necessary for the large-scale production and application of the arbuscular mycorrhizal fungi, and has important significance for agricultural sustainable development and ecological environment protection.
Disclosure of Invention
In order to solve the above problems, the present invention provides an arbuscular mycorrhizal fungi culture medium.
The components of the medium of the present invention are generally recognized in the art unless otherwise specified.
In order to achieve the above object, the technical solution of the present invention is as follows:
on one hand, the invention provides an arbuscular mycorrhizal fungi culture medium which comprises corn straw biochar, bentonite, zinc sulfite, magnesium sulfate, sodium alginate, vitamin B2, glutathione, glycylglycine, lignin, glucose and fructose.
Specifically, the culture medium comprises 5-15 parts of corn straw biochar, 20-40 parts of bentonite, 0.05-0.5 part of zinc sulfite, 0.1-0.5 part of magnesium sulfate, 0.1-0.5 part of sodium alginate, 21-5 parts of vitamin B, 1-3 parts of glutathione, 1-3 parts of glycylglycine, 3-7 parts of lignin, 3-7 parts of glucose and 3-7 parts of fructose.
Further specifically, the culture medium comprises 10 parts of corn straw biochar, 30 parts of bentonite, 0.1 part of zinc sulfite, 0.3 part of magnesium sulfate, 0.3 part of sodium alginate, 23 parts of vitamin B, 2 parts of glutathione, 2 parts of glycylglycine, 5 parts of lignin, 5 parts of glucose and 5 parts of fructose.
More specifically, the concentration of the zinc sulfite is 0.05-0.15mg/L, the concentration of the magnesium sulfate is 0.01-0.1mg/L, the concentration of the sodium alginate is 0.01-0.1mg/L, the concentration of the vitamin B2 is 10-15mg/L, the concentration of the glutathione is 3-7mg/L, the concentration of the glycylglycine is 3-7mg/L, the concentration of the glucose is 1200-1800mg/L, and the concentration of the fructose is 800-1200 mg/L.
More specifically, the concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
Further specifically, the preparation method of the corn straw biochar comprises the following steps: and (3) carbonizing the corn straws at 400 ℃ for 5-7h to prepare the corn straw biochar.
More specifically, the specific surface area of the corn stalk biochar is 100-500m 2 ·g -1 Preferably 100-200m 2 ·g -1 。
More specifically, the ratio of the corn stalk biochar to the bentonite is 1:2-5, and preferably 1: 3.
More specifically, the ratio of the glutathione to the glycylglycine is 1:1-3, preferably 1: 1.
In another aspect, the invention provides the use of the culture substrate described above in the culture of arbuscular mycorrhizal fungi.
Specifically, the arbuscular mycorrhizal fungus is arbuscular mycorrhizal fungus strain Rhizophagus irregularis M1.
In a further aspect, the invention provides an arbuscular mycorrhizal fungi culture obtained by culturing an arbuscular mycorrhizal fungi with the culture medium.
Specifically, the arbuscular mycorrhizal fungus is an arbuscular mycorrhizal fungus strain Rhizophagus irregularis M1.
In another aspect, the invention provides the application of the arbuscular mycorrhizal fungi culture in agricultural production or ecological environment restoration.
Compared with the prior art, the invention has the beneficial effects that:
the culture medium comprises corn straw biochar, bentonite, zinc sulfite, magnesium sulfate, sodium alginate, vitamin B2, glutathione, glycylglycine, lignin, glucose and fructose, and the arbuscular mycorrhizal fungi are cultured by the culture medium and are co-cultured with plants, so that the plant root system and microbial environment can be effectively improved, the heavy metal tolerance of the plants is improved, and the culture medium has important significance for agricultural sustainable development and ecological environment protection.
Drawings
FIG. 1 is a diagram showing the results of mycorrhiza infection rate tests.
FIG. 2 is a diagram showing the result of plant P content detection.
FIG. 3 is a diagram showing the result of Cr content measurement of the aerial parts of plants.
FIG. 4 is a diagram showing the result of Cr content measurement of a plant root system part.
FIG. 5 is a diagram showing the result of Cr transport coefficient detection.
Detailed Description
The present invention will be further illustrated in detail with reference to the following specific examples, which are not intended to limit the present invention but are merely illustrative thereof. The experimental methods used in the following examples are not specifically described, and the materials, reagents and the like used in the following examples are generally commercially available under the usual conditions without specific descriptions.
First, experimental material
1. The arbuscular mycorrhizal fungi is arbuscular mycorrhizal fungi strain Rhizopagus irregularis M1, purchased from China arbuscular mycorrhizal fungi germplasm resource library of agriculture and forestry academy of sciences, Beijing.
2. Bentonite: purchased from Sigma-Aldrich, cat # 682659.
3. Zinc sulfite: from Shanghai Ji to Biochemical technology, Inc., cat # Z80930.
4. Magnesium sulfate: purchased from Sigma-Aldrich, cat # M7506.
5. Sodium alginate: purchased from Sigma-Aldrich, cat # W201502.
6. Vitamin B2: purchased from national pharmaceutical group chemical agents, Inc., having a product number CATO-CCFD 200001.
7. Glutathione: purchased from national pharmaceutical group chemical agents limited, cat # TG 00731G.
8. Glycylglycine: purchased from national pharmaceutical group chemical agents, ltd, cat cvd 200626.
9. Lignin: purchased from national pharmaceutical group chemical agents limited, cat # a 37095901.
10. Glucose: available from Kaisnlei technologies, Inc. of Beijing under the trade designation MFCD 00148913.
11. Fructose: purchased from national pharmaceutical group chemical agents limited, cat # 63003034.
Example 1 an arbuscular mycorrhizal fungi culture substrate
The culture medium comprises 10 parts of corn straw biochar, 30 parts of bentonite, 0.1 part of zinc sulfite, 0.3 part of magnesium sulfate, 0.3 part of sodium alginate, 23 parts of vitamin B, 2 parts of glutathione, 2 parts of glycylglycine, 5 parts of lignin, 5 parts of glucose and 5 parts of fructose.
The concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
The preparation method of the corn straw biochar comprises the following steps: cleaning corn stalks, naturally drying in the air, cutting into pieces, and crushing in a crusher. And (3) putting the crushed corn straws into an oven, and drying for 12 hours at 70-80 ℃ until the crushed corn straws are completely dried. Placing the dried corn stalks in a muffle furnace, heating for 6h at 400 ℃, taking out, and sieving by a 0.154mm sieve. The specific surface area of the prepared corn straw biochar is 109.3m 2 ·g -1 。
Example 2 an arbuscular mycorrhizal fungi culture substrate
The culture medium comprises 5 parts of corn straw biochar, 20 parts of bentonite, 0.5 part of zinc sulfite, 0.5 part of magnesium sulfate, 0.5 part of sodium alginate, 25 parts of vitamin B, 3 parts of glutathione, 3 parts of glycylglycine, 7 parts of lignin, 7 parts of glucose and 7 parts of fructose.
The concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
The preparation method of the corn stalk biochar is the same as that in example 1.
Example 3 an arbuscular mycorrhizal fungi culture substrate
The culture medium comprises 15 parts of corn straw biochar, 40 parts of bentonite, 0.05 part of zinc sulfite, 0.1 part of magnesium sulfate, 0.1 part of sodium alginate, 21 parts of vitamin B, 1 part of glutathione, 1 part of glycylglycine, 3 parts of lignin, 3 parts of glucose and 3 parts of fructose.
The concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
The preparation method of the corn stalk biochar is the same as that in example 1.
Comparative example 1. an arbuscular mycorrhizal fungi culture substrate
The culture medium comprises 10 parts of corn straw biochar, 30 parts of bentonite, 0.1 part of zinc sulfite, 0.3 part of magnesium sulfate, 0.3 part of sodium alginate, 23 parts of vitamin B, 2 parts of glutathione, 2 parts of glycylglycine, 5 parts of lignin, 5 parts of glucose and 5 parts of fructose.
The concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
The preparation method of the corn straw biochar comprises the following steps: cleaning corn stalks, naturally drying in the air, cutting into pieces, and crushing in a crusher. And (3) putting the crushed corn straws into an oven, and drying for 12 hours at 70-80 ℃ until the crushed corn straws are completely dried. Placing the dried corn stalks in a muffle furnace, heating for 6h at 500 ℃, taking out, and sieving by a 0.154mm sieve. The specific surface area of the prepared corn straw biochar is 482.5m 2 ·g -1 。
Comparative example 2. an arbuscular mycorrhizal fungi culture substrate
The culture medium comprises 10 parts of corn straw biochar, 30 parts of bentonite, 0.1 part of zinc sulfite, 0.3 part of magnesium sulfate, 0.3 part of sodium alginate, 23 parts of vitamin B, 4 parts of glutathione, 5 parts of lignin, 5 parts of glucose and 5 parts of fructose.
The concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
The preparation method of the corn stalk biochar is the same as that in example 1.
Comparative example 3 an arbuscular mycorrhizal fungi culture medium
The culture medium comprises 10 parts of corn straw biochar, 30 parts of bentonite, 0.1 part of zinc sulfite, 0.3 part of magnesium sulfate, 0.3 part of sodium alginate, 23 parts of vitamin B, 4 parts of glycylglycine, 5 parts of lignin, 5 parts of glucose and 5 parts of fructose.
Wherein the concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
The preparation method of the corn stalk biochar is the same as that in example 1.
Comparative example 4. arbuscular mycorrhizal fungi culture medium
The culture medium comprises 40 parts of bentonite, 0.1 part of zinc sulfite, 0.3 part of magnesium sulfate, 0.3 part of sodium alginate, 23 parts of vitamin B, 2 parts of glutathione, 2 parts of glycylglycine, 5 parts of lignin, 5 parts of glucose and 5 parts of fructose.
The concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
Experimental example 1.
1. Preparing a heavy metal soil matrix: and (5) taking the soil, naturally drying, and sieving by using a 4mm sieve to obtain the soil matrix. Heavy metal Cr in K 2 CrO 4 Adding the solution into the soil matrix in the form of solution, wherein the concentration of soil Cr is 20mg/kg, and preparing the heavy metal Cr soil matrix.
2. Preparing an arbuscular mycorrhizal fungal inoculant: respectively taking 400g of the heavy metal soil matrix prepared in the step 1, mixing the heavy metal soil matrix with the arbuscular mycorrhizal fungi culture matrixes of the examples 1-3 and the comparative examples 1-4 to obtain a mixed matrix, and inoculating arbuscular mycorrhizal fungi on the mixed matrix to ensure that the density of inoculated spores of the arbuscular mycorrhizal fungi is 10/g, wherein the densities are respectively marked as examples 1-3 and comparative examples 1-4; and meanwhile, setting a blank control group (400 g of the heavy metal soil matrix prepared in the step 1 is taken and inoculated with arbuscular mycorrhizal fungi, so that the density of inoculated spores is 10/g).
3. The experimental method comprises the following steps: and (3) taking 8 cultured pot plants, adding 600g of the heavy metal soil substrate prepared in the step (1) into each cultured pot plant, and putting the mixed substrate containing the arbuscular mycorrhizal fungi prepared in the step (2) in the upper layer of the pot plant and the mixed substrate containing the arbuscular mycorrhizal fungi prepared in the step (1-3) and the mixed substrate containing the arbuscular mycorrhizal fungi prepared in the step (1-4) in the upper layer of the pot plant. 10 alfalfa seedlings were planted in each pot, and after two weeks, thinning was performed, 3 plants with consistent growth were kept, and the rest were removed. The water was weighed daily to maintain the moisture content at 15%. The potted plants were placed in a climatic chamber with an indoor illumination intensity of 700. mu. mol/m 2 /s -1 And in the daytime: the black night is 25 ℃: 20 ℃ and 16 h: 8h, relative humidity 70%. The plants were harvested two months after growth.
4. And (3) harvesting a sample: harvesting the overground part and the root system of the alfalfa respectively, screening out the root system, washing the root system with tap water, and storing a small amount of the root system in 50% alcohol for determining the mycorrhiza infection rate. And (3) putting the rest samples in an oven for deactivation of enzymes at 105 ℃ for 30min, drying at 70 ℃ for 48h until the weight is constant, and weighing and recording. Meanwhile, the soil sample is stored and naturally dried to be tested.
5. And respectively measuring the mycorrhiza infection rate, the plant element content and the soil Cr form by referring to a detection method known in the prior art.
(1) Determination of mycorrhiza infection rate: 10 percent of KOH, water bath is carried out for 30min at 90 ℃ for transparent plant roots, water washing is carried out, 2 percent of HCl is acidified for 5min at normal temperature, then water bath dyeing is carried out for 30min at 90 ℃ by 0.05 percent of Tribenezene blue, and finally lactic acid glycerol is used for decoloring. After the decolorization is completed, root segments of 50 roots and 0.5cm in length are randomly selected and sliced, observation is carried out under a microscope of 200 times, infection grades are recorded according to mycorrhizal infection and arbuscular abundance grading standards, and mycorrhizal infection rate is calculated by adopting Mycocalc. The results of the detection are shown in FIG. 1.
As can be seen from FIG. 1, the arbuscular mycorrhizal fungi culture medium provided by the invention can significantly improve the mycorrhizal infection rate of alfalfa and promote the growth of plants and the symbiotic relationship between mycorrhizal fungi and plants.
(2) And (3) measuring the content of the plant P: weighing a certain amount of dry aerial parts of the plants, putting the dry aerial parts into a microwave digestion tube, adding 10mL of pure nitric acid, standing overnight for pre-digestion, and then digesting by using a microwave digestion instrument. Digestion program: climbing to 120 deg.C for 8min, and maintaining for 3 min; climbing to 160 ℃ for 11min, and keeping for 5 min; the temperature is raised to 180 ℃ for 7min and kept for 40 min. And (4) driving the acid of the digestion solution to the residual 1mL at 140 ℃, transferring the digestion solution to a centrifuge tube for constant volume, filtering the digestion solution and waiting for the determination on a machine. The plant sample P concentration was determined using an inductively coupled plasma emission spectrometer (ICP-OES). The results of the detection are shown in FIG. 2.
As shown in FIG. 2, the arbuscular mycorrhizal fungi culture medium provided by the invention can be used for remarkably increasing the P content of plants and promoting the P absorption of the plants.
(3) And (3) determination of Cr content of the plants: weighing a certain amount of dry samples of the overground parts and the root parts of the plants into a microwave digestion tube, adding 10mL of pure nitric acid, standing overnight for pre-digestion, and then digesting by a microwave digestion instrument. Digestion program: climbing to 120 deg.C for 8min, and maintaining for 3 min; climbing to 160 ℃ for 11min, and keeping for 5 min; the temperature is raised to 180 ℃ for 7min, and the temperature is kept for 40 min. And (4) driving the acid of the digestion solution to the residual 1mL at 140 ℃, transferring the digestion solution to a centrifuge tube for constant volume, filtering the digestion solution and waiting for the determination on a machine. And measuring the Cr concentration of the plant sample by using an inductively coupled plasma mass spectrometer (ICP-MS) and calculating the Cr transport coefficient (the Cr transport coefficient is the ratio of the Cr content of the overground part of the plant to the Cr content of the root part of the plant). The detection results are shown in fig. 3-5.
As can be seen from FIGS. 3-5, the arbuscular mycorrhizal fungi culture medium of the invention can significantly reduce the Cr content of the overground part and the root system of the plant, and simultaneously inhibit the transfer of Cr from the root system to the overground part, and alleviate the toxic action of chromium in the plant.
The above examples are only illustrative of several embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the spirit of the invention, which falls within the scope of the invention as defined in the appended claims.
Claims (16)
1. An arbuscular mycorrhizal fungi culture medium, which is characterized in that: the culture medium comprises corn stalk biochar, bentonite, zinc sulfite, magnesium sulfate, sodium alginate, vitamin B2, glutathione, glycylglycine, lignin, glucose and fructose.
2. The culture substrate according to claim 1, wherein: the culture medium comprises 5-15 parts of corn straw biochar, 20-40 parts of bentonite, 0.05-0.5 part of zinc sulfite, 0.1-0.5 part of magnesium sulfate, 0.1-0.5 part of sodium alginate, 0.1-5 parts of vitamin B21-5 parts, 1-3 parts of glutathione, 1-3 parts of glycylglycine, 3-7 parts of lignin, 3-7 parts of glucose and 3-7 parts of fructose.
3. The culture substrate according to claim 2, wherein: the culture medium comprises 10 parts of corn straw biochar, 30 parts of bentonite, 0.1 part of zinc sulfite, 0.3 part of magnesium sulfate, 0.3 part of sodium alginate, 23 parts of vitamin B, 2 parts of glutathione, 2 parts of glycylglycine, 5 parts of lignin, 5 parts of glucose and 5 parts of fructose.
4. The culture substrate according to claim 2, wherein: the concentration of the zinc sulfite is 0.05-0.15mg/L, the concentration of the magnesium sulfate is 0.01-0.1mg/L, the concentration of the sodium alginate is 0.01-0.1mg/L, the concentration of the vitamin B2 is 10-15mg/L, the concentration of the glutathione is 3-7mg/L, the concentration of the glycylglycine is 3-7mg/L, the concentration of the glucose is 1800mg/L and the concentration of the fructose is 800 mg/L.
5. The culture substrate according to claim 4, wherein: the concentration of the zinc sulfite is 0.1mg/L, the concentration of the magnesium sulfate is 0.05mg/L, the concentration of the sodium alginate is 0.05mg/L, the concentration of the vitamin B2 is 12mg/L, the concentration of the glutathione is 5mg/L, the concentration of the glycylglycine is 5mg/L, the concentration of the glucose is 1500mg/L, and the concentration of the fructose is 1000 mg/L.
6. The culture substrate according to claim 1, wherein: the preparation method of the corn straw biochar comprises the following steps: and (3) putting the corn straws at 400 ℃ for carbonization for 5-7h to prepare the corn straw biochar.
7. The culture substrate according to claim 6, wherein: the specific surface area of the corn straw biochar is 100-500m 2 ·g -1 。
8. The culture substrate according to claim 7, wherein: the specific surface area of the corn straw biochar is 100-200m 2 ·g -1 。
9. The culture substrate according to claim 1, wherein: the ratio of the corn straw biochar to the bentonite is 1: 2-5.
10. The culture substrate according to claim 9, wherein: the ratio of the corn straw biochar to the bentonite is 1: 3.
11. The culture substrate according to claim 1, wherein: the ratio of the glutathione to the glycylglycine is 1: 1-3.
12. The culture substrate according to claim 11, wherein: the ratio of the glutathione to the glycylglycine is 1: 1.
13. Use of a culture substrate according to any one of claims 1 to 12 for the cultivation of arbuscular mycorrhizal fungi.
14. Use according to claim 13, characterized in that: the arbuscular mycorrhizal fungi is arbuscular mycorrhizal fungi strain Rhizophagus irregularis M1.
15. An arbuscular mycorrhizal fungi culture characterized by: the culture is obtained by culturing arbuscular mycorrhizal fungi by the culture substrate of any one of claims 1-12; the arbuscular mycorrhizal fungi is an arbuscular mycorrhizal fungi strain Rhizopagus irregularis M1.
16. Use of the culture of claim 15 for agricultural production or ecological environment restoration.
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| CN115504839A (en) * | 2022-09-27 | 2022-12-23 | 苏州农业职业技术学院 | Microbial compound bacterial fertilizer and preparation method thereof |
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| CN109197385A (en) * | 2018-09-20 | 2019-01-15 | 浙江世佳科技有限公司 | A kind of arbuscular mycorrhizal fungi culture substrate |
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| CN101565689A (en) * | 2009-05-26 | 2009-10-28 | 浙江师范大学 | Production method for high-density pure arbuscular mycorrhizal fungal spore |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115504839A (en) * | 2022-09-27 | 2022-12-23 | 苏州农业职业技术学院 | Microbial compound bacterial fertilizer and preparation method thereof |
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