CN114933962A - Nucleic acid detection device - Google Patents

Nucleic acid detection device Download PDF

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Publication number
CN114933962A
CN114933962A CN202210670949.7A CN202210670949A CN114933962A CN 114933962 A CN114933962 A CN 114933962A CN 202210670949 A CN202210670949 A CN 202210670949A CN 114933962 A CN114933962 A CN 114933962A
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China
Prior art keywords
test tube
tube body
reagent
push rod
cavity
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CN202210670949.7A
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Chinese (zh)
Inventor
卢先东
李玮
何永斌
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Ningbo Aigene Technology Co ltd
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Ningbo Aigene Technology Co ltd
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Priority to CN202210670949.7A priority Critical patent/CN114933962A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a nucleic acid detection device, which comprises a test tube and a heating module, wherein the test tube comprises a test tube body, a push rod arranged in an inner cavity of the test tube body in a sliding manner, a flexible sealing cover covering the top of the test tube body and a base arranged at the bottom of the test tube body, the bottom of the inner cavity of the test tube body is upwards provided with a thimble, an accommodating cavity used for pre-loading reagent paper is arranged in the side wall of the test tube body, the bottom of the push rod is provided with a diluent cavity pre-loaded with a diluent, the bottom of the diluent cavity is provided with a sealing membrane, the heating module is used for heating the reagent cavity in the base, when the device is used, the push rod can be pushed downwards by pressing the flexible sealing cover so that the sealing membrane is broken by the thimble, the diluent flows into the reagent cavity, the reagent liquid in the reagent cavity enters the accommodating cavity from the reagent cavity, and the reagent liquid permeates upwards along the reagent paper and displays a detection result on the reagent paper. The nucleic acid detection device is convenient to operate and high in safety.

Description

Nucleic acid detection device
Technical Field
The invention relates to the technical field of assay, in particular to a nucleic acid detection device.
Background
The substance for detecting the nucleic acid is nucleic acid of the virus, the nucleic acid detection is to find out whether the respiratory tract specimen, blood or feces of the patient have nucleic acid of the virus invaded from the outside to determine whether the respiratory tract specimen, the blood or the feces are infected by the new coronavirus, so that once the nucleic acid is detected to be positive, the fact that the virus exists in the patient can be proved, after the new coronavirus infects the human body, the new coronavirus is propagated in a respiratory tract system firstly, so that whether the human body is infected by the virus can be judged by detecting the viral nucleic acid in the respiratory tract epithelial exfoliated cells, and the nucleic acid detection positive can be used as a gold standard for diagnosing the infection of the novel coronavirus.
Disclosure of Invention
The invention aims to provide a nucleic acid detection device which is convenient to operate and high in safety.
The present invention provides a nucleic acid detecting apparatus including a test tube including:
the test tube comprises a test tube body, wherein the top of the test tube body is provided with an opening, the bottom of the test tube body is provided with a closed opening, the test tube body is provided with an inner cavity, the bottom of the inner cavity is upwards provided with a thimble, and the bottom end of the test tube body is provided with a circulation hole communicated with the inner cavity; an accommodating cavity used for pre-loading reagent paper is formed in the side wall of the test tube body along the length direction of the side wall, a flow channel communicated with the accommodating cavity and used for upward permeation of reagent liquid is arranged at the bottom of the test tube body, and one end of the flow channel penetrates through the bottom end of the test tube body to form a reagent hole;
the flexible sealing cover is covered on the top of the test tube body so as to seal the top opening of the test tube body;
the push rod is arranged in a sliding mode along the length direction of the inner cavity, the top of the push rod extends into the flexible sealing cover, the bottom of the push rod is located above the ejector pin, a diluent cavity filled with diluent in advance is formed in the bottom of the push rod, and a sealing film is arranged at the bottom of the diluent cavity;
the base is detachably connected to the bottom of the test tube body, a sealing structure is arranged between the base and the bottom of the test tube body, and a reagent cavity for placing a pre-filled detection reagent is arranged in the base;
when the flexible sealing cover is pressed, the flexible sealing cover is used for pushing the push rod downwards to enable the sealing film to be broken through by the ejector pin, diluent flows downwards into the reagent cavity from the circulation hole, reagent liquid in the reagent cavity enters the accommodating cavity from the reagent hole through the circulation channel, and the reagent liquid penetrates upwards along the reagent paper and displays a detection result on the reagent paper.
Compared with the prior art, the invention designs the convenient nucleic acid detection device, the test tube comprises a test tube body, a flexible sealing cover arranged at the top of the test tube body, a push rod arranged in an inner cavity of the test tube body and a base arranged at the bottom of the test tube body, the flexible sealing cover covers the top of the test tube body so as to seal an opening at the top of the test tube body, the base is connected at the bottom of the test tube body in a sealing way, the test tube body, the flexible sealing cover and the base enclose to form a sealing cavity, and the pre-loaded reagent paper is arranged in an accommodating cavity in the side wall of the test tube body, namely the pre-loaded reagent paper is arranged in the sealing cavity, so that the reagent paper is not polluted before detection, the accuracy of a detection result can be ensured, and a false positive result is avoided; meanwhile, a reagent cavity for placing a pre-filled detection reagent is arranged in the base, namely the pre-filled detection reagent is also placed in the sealed cavity, so that the pre-filled detection reagent in the reagent cavity is not polluted before use; when the reagent box is used, the base is taken down firstly, a nucleic acid sample to be detected is added, the base is installed again, the reagent box is heated by heating equipment, the flexible sealing cover is flexible and can deform when being pressed, so that the flexible sealing cover can be pressed to push the push rod downwards to enable the sealing film at the bottom end of the push rod to be broken by the ejector pin, a diluent flows into the reagent box downwards from the flow hole, a reagent liquid in the reagent box enters the accommodating cavity from the reagent hole through the flow channel, and the reagent liquid permeates upwards along the reagent paper and displays a detection result on the reagent paper; the detection device and the detection method can ensure no interference in the detection process and improve the accuracy of the detection result, and the device is convenient and quick to use, does not need to queue and pile for nucleic acid, avoids the poor infection risk in queuing, improves the safety of nucleic acid detection and is beneficial to normalization of nucleic acid detection.
As a preferable scheme of the invention, the inner wall of the test tube body is provided with a through groove communicated with the accommodating cavity, the groove bottom of the through groove is lower than a result display area of the reagent paper, and the through groove is used for stabilizing the air pressure in the test tube when the push rod slides and preventing the reagent liquid in the accommodating cavity from overflowing. In the detection process, in order to ensure that the air pressure in the sealed cavity is stable and the push rod can slide smoothly along the length direction of the test tube body, the inner wall of the test tube body is provided with the through groove communicated with the accommodating groove, and the through groove has the function of stabilizing the pressure and ensuring that reagent liquid upwards permeates onto the reagent paper at the bottom of the accommodating cavity instead of completely immersing the reagent paper.
As a preferable scheme of the invention, the push rod comprises a push rod and a piston head arranged at the bottom end of the push rod, the diluent cavity is formed at the bottom of the piston head, a rubber sleeve is wrapped outside the piston head, and the piston head is arranged in a sliding manner along the length direction of the inner wall of the test tube body through the rubber sleeve; the push rod is cross along the shape of the radial section of the test tube body, and the top of the push rod is provided with a pressing platform which is convenient for stable pressing. Through the design of the structure of the push rod, the push rod comprises a push rod and a piston head, the piston head is arranged at the bottom end of the push rod, a rubber sleeve wraps the outer part of the piston head, when the push rod slides in the test tube body, the push rod slides in a sealing way through the rubber sleeve and the inner wall of the test tube body, so that after a sealing film at the bottom of the push rod is broken, a diluent cannot flow above the piston head through a gap between the piston head and the inner wall of the test tube body, the diluent is guaranteed to flow downwards into a reagent cavity, and the smooth operation of nucleic acid detection is guaranteed; moreover, the push rod is cross-shaped instead of circular along the radial section of the test tube body, so that the weight of the push rod can be reduced; the top of push rod is provided with the platform of pressing of being convenient for stably press for when pressing flexible sealing lid, can stably press the platform of pressing of push rod on, make the push rod smooth, the steady slip.
Furthermore, the push rod is located push rod upper portion is provided with presses deformable gag lever post in back, the gag lever post erects on test tube body open-top's the terminal surface, the gag lever post is used for preventing the ejector pin removes downwards. Through the deformable gag lever post of design on barred body upper portion for the gag lever post is not being pressed the fore-stock and on test tube body open-top's upper end face, has guaranteed that the ejector pin can not move down in the transit, prevents that the envelope from being punctureed, and when pressing the ejector pin downwards, the gag lever post takes place deformation or rupture, and the ejector pin just can move down so that the envelope is broken by the top.
As a preferable scheme of the invention, the flexible sealing cover comprises a hard rotary cover, a flexible sleeve and a pressing handle, the hard rotary cover is in threaded connection with the outer wall of the opening at the top of the test tube body, an opening is formed in the middle of the hard rotary cover, the lower end of the flexible sleeve extends into the opening and is circumferentially and hermetically fixed with the opening, and the upper end of the flexible sleeve is hermetically fixed at the bottom of the pressing handle. Through the design of the structure of the flexible sealing cover, the lower end of the flexible sleeve extends into the opening of the hard spiral cover, and when the hard spiral cover is screwed to the top of the test tube body, the flexible sleeve extending into the hard spiral cover is pressed against the edge of the top opening of the test tube body, so that the hard spiral cover is in sealing connection with the top opening of the test tube body; the flexible sleeve is made of flexible materials and can deform when pressed; the pressing handle is further arranged, so that a user can press downwards conveniently, the contact area during pressing is increased, and the pressing is more stable.
As a preferable scheme of the invention, the test tube body is made of transparent materials, a plurality of edge surfaces are uniformly arranged on the outer wall of the test tube body in the circumferential direction, and one edge surface is right opposite to the result display surface of the reagent paper. The material of the test tube body is selected, and the test tube body is made of transparent material, so that the condition in the test tube body can be observed conveniently, and particularly the display detection result of the reagent paper can be observed; a plurality of faceted pebble is evenly set to test tube body's outer wall circumference, and one of them faceted pebble is just to the result display surface of reagent paper, and the transparent plane is more convenient for observe the display result on the reagent paper.
As a preferable scheme of the invention, a step structure is arranged at the bottom of the inner cavity along the circumferential direction of the thimble, and the step structure is used for abutting against the rubber sleeve to prevent the diluent from overflowing. Through set up the stair structure in the bottom of inner chamber along thimble circumference for when the piston head pushed down the inner chamber bottom, the rubber sleeve offsets with the stair structure, can strengthen the leakproofness between rubber sleeve and the stair structure, prevents that the diluent is excessive.
In a preferred embodiment of the present invention, a stopper is provided at a bottom end of the cuvette body so as to protrude downward, and the flow hole and the reagent hole are respectively located at both sides of the stopper. Set up the dog through the bottom at test tube body is outstanding downwards, and opening and reagent hole are located the both sides of dog respectively, when making the diluent flow down in the reagent chamber from the opening, the dog has the guide effect to the diluent, and the bottom of dog diminishes the back to the space of reagent chamber bottom, the diluent has the trend of flowing from opening wash-in to the reagent chamber downwards, again from the opposite side be the reagent hole, wash in the holding chamber that the pre-installation has reagent paper in the circulation passageway, guaranteed that reagent liquid enters into the holding chamber smoothly.
Furthermore, a limiting block used for limiting the flow rate of the reagent liquid is arranged on the side wall of the bottom of the accommodating cavity. In order to avoid reagent liquid to splash to reagent paper upper portion when rushing into the holding chamber, set up the stopper in the up lateral wall position of a bit in the bottom in holding chamber, the stopper can restrict the velocity of flow of reagent liquid, blocks reagent liquid in the bottom in reagent chamber for reagent liquid slowly upwards permeates from reagent paper bottom.
As a preferred scheme of the invention, the base is in threaded connection with the outer wall of the bottom of the test tube body, and a sealing ring is sleeved on the outer wall of the bottom of the test tube body; or, base threaded connection in on the bottom inner wall of test tube body, the cover is equipped with the sealing washer on the outer wall of base. Through base threaded connection on test tube body, realize the dismantlement between base and the test tube body and be connected, it is convenient to install and dismantle, establishes the sealing washer again on the bottom outer wall of test tube body, guarantees the sealing connection between base and the test tube body. Or, set up the external screw thread on the base, set up the internal thread on the test tube body, base threaded connection is equipped with the sealing washer on the outer wall of base on the bottom inner wall of test tube body, adopts this kind of sealing connection mode also in the protection scope of this application.
As a preferable scheme of the invention, the reagent chamber heating device further comprises a heating module, the heating module comprises a fixed seat, a slot for inserting the base is arranged on the upper surface of the fixed seat, a heat conduction block for heating the reagent chamber is arranged in the slot, and an electric heating block is arranged at the bottom of the heat conduction block. Through designing heating module, heating module includes the fixing base, and the upper surface of fixing base is provided with the slot that is used for the base to peg graft, during the use, inserts the base in the slot, and the heat conduction piece in the slot passes through the electric heating piece heating, is the outer wall looks adaptation with the reagent chamber with the structural design of heat conduction piece, can improve the heating effect in reagent chamber, and the heating is even. In the invention, the electric heating block can be electrified and heated through a dry battery, a lithium battery or a USB interface.
Drawings
FIG. 1 is a schematic view of the overall structure of a test tube according to the present invention;
FIG. 2 is a front view showing the overall structure of the test tube according to the present invention;
FIG. 3 is a cross-sectional view taken along the line A-A in FIG. 2;
FIG. 4 is a schematic view of the overall structure of the push rod of the present invention;
FIG. 5 is a first schematic view of the overall structure of the cuvette body according to the present invention;
FIG. 6 is a schematic view of the overall structure of the test tube body according to the present invention;
FIG. 7 is a front view showing the entire structure of the cuvette body according to the present invention;
FIG. 8 is a cross-sectional view taken along the line B-B in FIG. 7;
FIG. 9 is a schematic view of a connecting structure between a heating module and a base in the present invention.
Description of reference numerals:
1. a test tube; 2. a test tube body; 2.1, an inner cavity; 2.2, flow holes; 2.3, an accommodating cavity; 2.4, a flow channel; 2.5, reagent wells; 2.6, a through groove; 2.7, a prism surface; 2.8, a step structure; 2.9, a stop block; 2.10, a limiting block; 3. a thimble; 4. reagent paper; 5. a flexible sealing cover; 5.1, hard screw capping; 5.2, a flexible sleeve; 5.3, pressing a handle; 6. pushing the rod; 6.1, a push rod; 6.1.1, a limiting rod; 6.2, piston head; 6.3, rubber sleeve; 6.4, a diluent cavity; 6.5, sealing the membrane; 7. a base; 7.1, a reagent cavity; 7.2, a sealing ring; 8. a heating module; 8.1, fixing seats; 8.2, a slot; 8.3, heat conducting blocks; 8.4, an electric heating block.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in FIGS. 1 to 4, the present invention shows a nucleic acid detecting apparatus, which comprises a test tube 1 and a heating module 8, wherein the heating module 8 is used for heating the bottom of the test tube 1 to ensure the smooth detection.
Wherein, test tube 1 includes: the test tube comprises a test tube body 2, a flexible sealing cover 5, a push rod 6 and a base 7.
The top of the test tube body 2 is provided with an opening, the bottom of the test tube body 2 is provided with a closed opening, the test tube body 2 is provided with an inner cavity 2.1, the bottom of the inner cavity 2.1 is upwards provided with a thimble 3, and the bottom end of the test tube body 2 is provided with a circulation hole 2.2 communicated with the inner cavity 2.1; an accommodating cavity 2.3 used for pre-loading reagent paper 4 is formed in the side wall of the test tube body 2 along the length direction, a flow channel 2.4 communicated with the accommodating cavity 2.3 and used for upward permeation of reagent liquid is arranged at the bottom of the test tube body 2, and one end of the flow channel 2.4 penetrates through the bottom end of the test tube body 2 to form a reagent hole 2.5;
the flexible sealing cover 5 is covered on the top of the test tube body 2 to seal the top opening of the test tube body 2;
the push rod 6 is arranged in a sliding mode along the length direction of the inner cavity 2.1, the top of the push rod 6 extends into the flexible sealing cover 5, the bottom of the push rod 6 is located above the ejector pin 3, a diluent cavity 6.4 filled with diluent in advance is arranged at the bottom of the push rod 6, and a sealing film 6.5 is arranged at the bottom of the diluent cavity 6.4;
the base 7 is detachably connected to the bottom of the test tube body 2, a sealing structure is arranged between the base 7 and the bottom of the test tube body 2, and a reagent cavity 7.1 for placing a pre-filled detection reagent is arranged in the base 7;
when the flexible sealing cover 5 is pressed, the flexible sealing cover 5 is used for pushing the push rod 6 downwards to enable the sealing film 6.5 to be broken by the thimble 3, the diluent flows downwards into the reagent cavity 7.1 from the circulating hole 2.2, the reagent liquid in the reagent cavity 7.1 enters the accommodating cavity 2.3 from the reagent hole 2.5 through the circulating channel 2.4, and the reagent liquid penetrates upwards along the reagent paper 4 and displays the detection result on the reagent paper 4.
More specifically, as shown in fig. 5 and 6, the inner wall of the test tube body 2 is provided with a through groove 2.6 communicated with the accommodating cavity 2.3, the bottom of the through groove 2.6 is lower than the result display area of the reagent paper 4, and the through groove 2.6 is used for stabilizing the air pressure in the test tube when the push rod 6 slides and preventing the reagent liquid from overflowing from the accommodating cavity 2.3. In the detection process, in order to ensure stable air pressure in the sealed cavity and facilitate smooth sliding of the push rod 6 along the length direction of the test tube body 2, the inner wall of the test tube body 2 is provided with the through groove 2.6 communicated with the accommodating groove, and the through groove 2.6 has the functions of stabilizing pressure and ensuring that reagent liquid upwards permeates onto the reagent paper 4 at the bottom of the accommodating cavity 2.3 instead of completely immersing the reagent paper 4, so that the bottom of the through groove 2.6 is lower than a result display area of the reagent paper 4, so that the reagent liquid in the accommodating cavity 2.3 can overflow from the through groove 2.6 to the inner cavity 2.1 of the test tube body 2 when the reagent liquid is overfilled, and stable display of a detection result is ensured.
The push rod 6 comprises a push rod 6.1 and a piston head 6.2 arranged at the bottom end of the push rod 6.1, a diluent cavity 6.4 is formed in the bottom of the piston head 6.2, a rubber sleeve 6.3 wraps the outer part of the piston head 6.2, and the piston head 6.2 is arranged in a sliding mode along the length direction of the inner wall of the test tube body 2 through the rubber sleeve 6.3; the shape of push rod 6.1 along the radial cross-section of test tube body 2 is cross, and the top of push rod 6.1 is provided with the platform of pressing of being convenient for stably press. Through the design of the structure of the push rod 6, the push rod 6 comprises a push rod 6.1 and a piston head 6.2, the piston head 6.2 is arranged at the bottom end of the push rod 6.1, a rubber sleeve 6.3 wraps the outside of the piston head 6.2, and when the push rod 6 slides in the test tube body 2, the push rod slides in a sealing way through the rubber sleeve 6.3 and the inner wall of the test tube body 2, so that after a sealing membrane 6.5 at the bottom of the push rod 6 is broken, a diluent cannot flow to the upper part of the piston head 6.2 through a gap between the piston head 6.2 and the inner wall of the test tube body 2, the diluent is ensured to flow downwards into a reagent cavity 7.1, and the smooth operation of nucleic acid detection is ensured; moreover, the push rod 6.1 is cross-shaped instead of circular along the radial section of the test tube body 2, so that the weight of the push rod 6.1 can be reduced; the top of push rod 6.1 is provided with the platform of pressing of being convenient for stably press for when pressing flexible sealing lid 5, can stably press the platform of pressing of push rod 6 on, make push rod 6 smooth, steady slip.
Push rod 6.1 is located push rod 6.1 upper portion and is provided with deformable gag lever post 6.1.1 after pressing, and gag lever post 6.1.1 erects on the open-topped terminal surface in test tube body 2, and gag lever post 6.1.1 is used for preventing push rod 6 downstream. Through at bar upper portion design deformable gag lever post 6.1.1 for gag lever post 6.1.1 erects on the open-topped upper end face of test tube body 2 before not being pressed, has guaranteed that push rod 6 can not move down in the transit, prevents to seal membrane 6.5 and is punctured, when pushing down push rod 6, gag lever post 6.1.1 takes place deformation or rupture, and push rod 6 just can move down so that to seal membrane 6.5 and be punctured.
In the invention, the flexible sealing cover 5 comprises a hard rotary cover 5.1, a flexible sleeve 5.2 and a pressing handle 5.3, the hard rotary cover 5.1 is in threaded connection with the outer wall of the top opening of the test tube body 2, an opening is formed in the middle of the hard rotary cover 5.1, the lower end of the flexible sleeve 5.2 extends into the opening and is circumferentially and hermetically fixed with the opening, and the upper end of the flexible sleeve 5.2 is hermetically fixed at the bottom of the pressing handle 5.3. Through designing the structure of the flexible sealing cover 5, the lower end of the flexible sleeve 5.2 extends into an opening of the hard rotary cover 5.1, and when the hard rotary cover 5.1 is screwed to the top of the test tube body 2 in a threaded manner, the flexible sleeve 5.2 extending into the hard rotary cover 5.1 is pressed against the edge of the top opening of the test tube body 2, so that the hard rotary cover 5.1 is in sealing connection with the top opening of the test tube body 2; the flexible sleeve 5.2 is made of flexible materials and can deform when pressed; the pressing handle 5.3 is further arranged, so that a user can press downwards conveniently, the contact area during pressing is increased, and the pressing is more stable.
In the invention, the test tube body 2 is made of transparent materials, a plurality of edge surfaces 2.7 are uniformly arranged on the outer wall of the test tube body 2 in the circumferential direction, and one edge surface 2.7 is right opposite to a result display surface of the reagent paper 4. By selecting the material of the test tube body 2, the test tube body 2 is made of transparent material, so that the condition in the test tube body 2, particularly the display detection result of the reagent paper 4, can be observed conveniently; a plurality of faceted pebble 2.7 is evenly set to test tube body 2's outer wall circumference, and one of them faceted pebble 2.7 is just to the result display surface of reagent paper 4, and the transparent plane is more convenient for observe the display result on the reagent paper 4.
In addition, as shown in fig. 7 and 8, the bottom of the inner cavity 2.1 is provided with a step structure 2.8 along the circumferential direction of the thimble 3, and the step structure 2.8 is used for abutting against the rubber sleeve 6.3 to prevent the diluent from overflowing. Through set up stair structure 2.8 along thimble 3 circumference at the bottom of inner chamber 2.1 for when piston head 6.2 pushed down inner chamber 2.1 bottom, rubber sleeve 6.3 offsets with stair structure 2.8, can strengthen the leakproofness between rubber sleeve 6.3 and stair structure 2.8, prevents that the diluent is excessive.
In addition, the bottom end of the test tube body 2 is provided with a stop block 2.9 in a downward protruding mode, and the flow hole 2.2 and the reagent hole 2.5 are respectively positioned on two sides of the stop block 2.9. Through the bottom at test tube body 2 outstanding dog 2.9 that sets up downwards, and flow hole 2.2 and reagent hole 2.5 are located dog 2.9's both sides respectively, when making the thinner flow down to reagent chamber 7.1 in from flow hole 2.2, dog 2.9 has the guide effect to the thinner, and the bottom of dog 2.9 diminishes to the space of reagent chamber 7.1 bottom back, the thinner has the trend of flowing that dashes into in reagent chamber 7.1 downwards from flow hole 2.2, again from the opposite side be reagent hole 2.5, dash in the holding chamber 2.3 that is preloaded with reagent paper 4 in flow channel 2.4, guaranteed that reagent liquid enters into holding chamber 2.3 smoothly.
In addition, a limiting block 2.10 used for limiting the flow rate of the reagent liquid is arranged on the side wall of the bottom of the accommodating cavity 2.3. In order to avoid reagent liquid to splash to 4 upper portions of reagent paper when rushing into holding chamber 2.3, set up stopper 2.10 in the up lateral wall position of a bit in the bottom of holding chamber 2.3, stopper 2.10 can restrict the velocity of flow of reagent liquid, stop reagent liquid in the bottom of reagent chamber 7.1 for reagent liquid slowly upwards permeates from 4 bottoms of reagent paper.
In addition, the sealing film 6.5 is made of aluminum foil.
In the embodiment, the base 7 is in threaded connection with the outer wall of the bottom of the test tube body 2, and the outer wall of the bottom of the test tube body 2 is sleeved with the sealing ring 7.2; or, base 7 threaded connection is on the bottom inner wall of test tube body 2, and the cover is equipped with the sealing washer on base 7's the outer wall. Through 7 threaded connection of base on test tube body 2, realize being connected dismantled between base 7 and the test tube body 2, it is convenient to install and dismantle, establishes sealing washer 7.2 again at the cover on the bottom outer wall of test tube body 2, guarantees the sealing connection between base 7 and the test tube body 2. Or, set up the external screw thread on the base 7, set up the internal thread on the test tube body 2, base 7 threaded connection is on test tube body 2's bottom inner wall, and the cover is equipped with sealing washer 7.2 on base 7's the outer wall, adopts this kind of sealing connection mode also in the protection scope of this application.
In order to facilitate the installation and the disassembly of the base 7 to be convenient, the outer wall of the base 7 is also provided with anti-slip lines, and when the anti-slip lines are screwed or taken down, the hand friction force is increased, so that the operation is convenient.
As shown in fig. 9, the present invention further includes a heating module 8, the heating module 8 includes a fixing base 8.1, a slot 8.2 for inserting the base 7 is disposed on an upper surface of the fixing base 8.1, a heat conduction block 8.3 for heating the reagent chamber 7.1 is disposed in the slot 8.2, and an electric heating block 8.4 is disposed at a bottom of the heat conduction block 8.3. Through designing heating module 8, heating module 8 includes fixing base 8.1, fixing base 8.1's upper surface is provided with the slot 8.2 that is used for base 7 to peg graft, during the use, insert slot 8.2 with base 7 in, heat conduction piece 8.3 in the slot 8.2 heats through electric heating piece 8.4, the structural design with heat conduction piece 8.3 is for the outer wall looks adaptation with reagent chamber 7.1, can improve reagent chamber 7.1's heating effect, the heating is even. In the invention, the electric heating block 8.4 can be electrified and heated through a dry battery, a lithium battery or a USB interface.
When the test tube is used, firstly, the base 7 is screwed down from the test tube body 2, then a nucleic acid sample to be detected is added into the reagent cavity 7.1 of the base 7, then the base 7 is remounted at the bottom of the test tube body 2, the base 7 is inserted into the slot 8.2 of the fixed seat 8.1 of the heating module 8, after being heated by the electric heating block 8.4, the flexible sealing cover 5 is pressed downwards to push the push rod 6 to move downwards, so that the sealing membrane 6.5 is burst, the diluent flows downwards into the reagent cavity 7.1 from the circulation hole 2.2, and the reagent liquid in the reagent cavity 7.1 is flushed into the accommodating cavity 2.3 from the reagent hole 2.5 and the circulation channel 2.4, the reagent liquid in the accommodating cavity 2.3 permeates upwards along the pre-filled reagent paper 4, and a detection result is displayed on the reagent paper 4, and because the test tube body 2 is transparent, a detector can directly check the detection result through the edge surface 2.7, thereby being very convenient.
In summary, the invention has the following advantages:
1) the test tube comprises a test tube body 2, a flexible sealing cover 5 arranged at the top of the test tube body 2, a push rod 6 arranged in an inner cavity 2.1 of the test tube body 2 and a base 7 arranged at the bottom of the test tube body 2, wherein the flexible sealing cover 5 covers the top of the test tube body 2 so as to seal the top opening of the test tube body 2, the base 7 is connected to the bottom of the test tube body 2 in a sealing way, the test tube body 2, the flexible sealing cover 5 and the base 7 enclose to form a sealing cavity, and pre-loaded reagent paper 4 is arranged in an accommodating cavity 2.3 in the side wall of the test tube body 2, namely the pre-loaded reagent paper 4 is arranged in the sealing cavity, so that the reagent paper 4 is not polluted before detection, the accuracy of a detection result can be ensured, and a false positive result is avoided;
2) meanwhile, a reagent cavity 7.1 for placing a pre-filled detection reagent is arranged in the base 7, namely the pre-filled detection reagent is also placed in the sealed cavity, so that the pre-filled detection reagent in the reagent cavity 7.1 is ensured to be not polluted before use; when the reagent box is used, the base 7 is taken down firstly, a nucleic acid sample to be detected is added, the base 7 is installed again, the reagent cavity 7.1 is heated by heating equipment, the flexible sealing cover 5 is flexible and can deform when being pressed, so that the flexible sealing cover 5 can be pressed to push the push rod 6 downwards to enable the sealing film 6.5 at the bottom end of the push rod 6 to be broken by the thimble 3, diluent flows downwards into the reagent cavity 7.1 from the flow hole 2.2, reagent liquid in the reagent cavity 7.1 flows into the accommodating cavity 2.3 from the reagent hole 2.5 through the flow channel 2.4, and the reagent liquid permeates upwards along the reagent paper 4 and displays a detection result on the reagent paper 4; the detection device and the detection method can ensure no interference in the detection process, improve the accuracy of the detection result, are convenient and quick when in use, do not need to queue and pile for nucleic acid, avoid the poor infection risk in queuing, improve the safety of nucleic acid detection and are beneficial to normalization of nucleic acid detection;
3) heating module 8 includes fixing base 8.1, fixing base 8.1's upper surface is provided with the slot 8.2 that is used for base 7 to peg graft, in using, insert slot 8.2 with base 7 in, heat conduction piece 8.3 in the slot 8.2 is through electric heating piece 8.4 ohmic heating, be the outer wall looks adaptation with reagent chamber 7.1 with heat conduction piece 8.3's structural design, can improve reagent chamber 7.1's heating effect, the heating is even, furthermore, electric heating piece 8.4 accessible dry battery, the lithium cell or carry out ohmic heating through the USB interface, and is also very convenient, and low cost.
It should be noted that all the directional indicators (such as upper, lower, left, right, front, rear, inner and outer … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the figure), and if the specific posture is changed, the directional indicator is changed accordingly.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integral; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made without departing from the spirit and scope of the invention.

Claims (10)

1. A nucleic acid detecting apparatus, comprising a test tube (1), wherein the test tube (1) comprises:
the test tube comprises a test tube body (2), wherein the top of the test tube body (2) is provided with an opening, the bottom of the test tube body (2) is provided with a closed opening, the test tube body (2) is provided with an inner cavity (2.1), the bottom of the inner cavity (2.1) is upwards provided with a thimble (3), and the bottom end of the test tube body (2) is provided with a circulation hole (2.2) communicated with the inner cavity (2.1); an accommodating cavity (2.3) used for pre-installing reagent paper (4) is formed in the side wall of the test tube body (2) along the length direction of the side wall, a flow channel (2.4) communicated with the accommodating cavity (2.3) and used for upward permeation of reagent liquid is arranged at the bottom of the test tube body (2), and one end of the flow channel (2.4) penetrates through the bottom end of the test tube body (2) to form a reagent hole (2.5);
the flexible sealing cover (5) is covered on the top of the test tube body (2) so as to seal the top opening of the test tube body (2);
the push rod (6) is arranged in a sliding mode along the length direction of the inner cavity (2.1), the top of the push rod (6) extends into the flexible sealing cover (5), the bottom of the push rod (6) is located above the ejector pin (3), a diluent cavity (6.4) filled with a diluent in advance is formed in the bottom of the push rod (6), and a sealing film (6.5) is arranged at the bottom of the diluent cavity (6.4);
the base (7) is detachably connected to the bottom of the test tube body (2), a sealing structure is arranged between the base (7) and the bottom of the test tube body (2), and a reagent cavity (7.1) for placing a pre-filled detection reagent is arranged in the base (7);
when the flexible sealing cover (5) is pressed, the flexible sealing cover (5) is used for pushing the push rod (6) downwards to enable the sealing membrane (6.5) to be broken by the ejector pin (3), diluent flows downwards into the reagent cavity (7.1) from the circulation hole (2.2), reagent liquid in the reagent cavity (7.1) enters the accommodating cavity (2.3) from the reagent hole (2.5) through the circulation channel (2.4), and the reagent liquid penetrates upwards along the reagent paper (4) and displays a detection result on the reagent paper (4).
2. The nucleic acid detecting apparatus according to claim 1, characterized in that: set up on the inner wall of test tube body (2) with logical groove (2.6) that holding chamber (2.3) are linked together, the tank bottom that leads to groove (2.6) is less than the result display area of reagent paper (4), lead to groove (2.6) and be used for when pushing away stick (6) and sliding stablize atmospheric pressure in the test tube and be used for preventing reagent liquid overflow in holding chamber (2.3).
3. The nucleic acid detecting apparatus according to claim 2, characterized in that: push rod (6) include push rod (6.1) and set up in piston head (6.2) of push rod (6.1) bottom, the bottom of piston head (6.2) has been seted up diluent chamber (6.4), the outside parcel of piston head (6.2) has rubber sleeve (6.3), piston head (6.2) are passed through rubber sleeve (6.3) are followed test tube body (2) inner wall's length direction slides and sets up.
4. The nucleic acid detecting apparatus according to claim 3, characterized in that: push rod (6.1) are located push rod (6.1) upper portion is provided with deformable gag lever post (6.1.1) after pressing, gag lever post (6.1.1) erect on test tube body (2) open-top's terminal surface, gag lever post (6.1.1) are used for preventing push rod (6) downstream.
5. The nucleic acid detecting apparatus according to claim 1, characterized in that: flexible sealing lid (5) include stereoplasm spiral cover (5.1), flexible cover (5.2) and press (5.3), stereoplasm spiral cover (5.1) with test tube body's (2) open-top outer wall threaded connection, the middle part of stereoplasm spiral cover (5.1) is provided with the trompil, the lower extreme of flexible cover (5.2) stretches into in the trompil and with trompil circumference is sealed fixedly, the upper end seal of flexible cover (5.2) is fixed in press the bottom of (5.3).
6. The nucleic acid detecting apparatus according to claim 1, characterized in that: test tube body (2) adopt transparent material to make, circumference evenly is provided with a plurality of faceted pebble (2.7) on the outer wall of test tube body (2), one of them faceted pebble (2.7) just to the result display surface of reagent paper (4).
7. The nucleic acid detecting apparatus according to claim 3, wherein: the bottom of the inner cavity (2.1) is provided with a step structure (2.8) along the circumferential direction of the thimble (3), and the step structure (2.8) is used for being abutted against the rubber sleeve (6.3) to prevent the diluent from overflowing.
8. The nucleic acid detecting apparatus according to claim 1, characterized in that: the bottom end of the test tube body (2) is provided with a stop block (2.9) in a downward protruding mode, and the flow hole (2.2) and the reagent hole (2.5) are located on two sides of the stop block (2.9) respectively.
9. The nucleic acid detecting apparatus according to claim 1, characterized in that: the base (7) is in threaded connection with the outer wall of the bottom of the test tube body (2), and a sealing ring (7.2) is sleeved on the outer wall of the bottom of the test tube body (2); or, base (7) threaded connection in on the bottom inner wall of test tube body (2), the cover is equipped with the sealing washer on the outer wall of base (7).
10. The nucleic acid detecting apparatus according to any one of claims 1 to 9, characterized in that: still include heating module (8), heating module (8) are including fixing base (8.1), the upper surface of fixing base (8.1) is provided with and is used for slot (8.2) that base (7) are pegged graft, it is right to be provided with in slot (8.2) be used for heat conduction piece (8.3) of reagent chamber (7.1) heating, the bottom of heat conduction piece (8.3) is provided with electrical heating piece (8.4).
CN202210670949.7A 2022-06-10 2022-06-10 Nucleic acid detection device Pending CN114933962A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210670949.7A CN114933962A (en) 2022-06-10 2022-06-10 Nucleic acid detection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210670949.7A CN114933962A (en) 2022-06-10 2022-06-10 Nucleic acid detection device

Publications (1)

Publication Number Publication Date
CN114933962A true CN114933962A (en) 2022-08-23

Family

ID=82866699

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210670949.7A Pending CN114933962A (en) 2022-06-10 2022-06-10 Nucleic acid detection device

Country Status (1)

Country Link
CN (1) CN114933962A (en)

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