CN114933664B - Extraction method of pentosan - Google Patents

Extraction method of pentosan Download PDF

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CN114933664B
CN114933664B CN202210670285.4A CN202210670285A CN114933664B CN 114933664 B CN114933664 B CN 114933664B CN 202210670285 A CN202210670285 A CN 202210670285A CN 114933664 B CN114933664 B CN 114933664B
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pentosan
fermentation
drum
pipe
tank
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CN114933664A (en
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张庆玲
冯军伟
董必辉
段兰兰
闫佳佳
刘世欣
周志强
戴厚同
董得平
李林海
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Henan Feitian Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0057—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Xylans, i.e. xylosaccharide, e.g. arabinoxylan, arabinofuronan, pentosans; (beta-1,3)(beta-1,4)-D-Xylans, e.g. rhodymenans; Hemicellulose; Derivatives thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F29/00—Mixers with rotating receptacles
    • B01F29/60—Mixers with rotating receptacles rotating about a horizontal or inclined axis, e.g. drum mixers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Sustainable Development (AREA)
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Abstract

The invention belongs to the field of pentosan preparation, and particularly relates to an extraction method of pentosan, which comprises the following steps: (1) Grinding wheat into flour, adding water, homogenizing and heating at 20-35 deg.C for 10-12 hr, separating by three-phase turbo centrifuge 3 to obtain coarse pentosan liquid, wheat gluten liquid and rest starch milk; curing the wheat gluten liquid, dehydrating and drying to obtain a finished wheat gluten product; the method has high extraction rate of pentosan and can ensure the content of part of vitamins in the processing process.

Description

Extraction method of pentosan
Technical Field
The invention belongs to the field of pentosan preparation, and particularly relates to an extraction method of pentosan.
Background
Pentosan is mainly a pentose polymer, and researches in recent years show that pentosan not only reduces blood sugar and blood fat, but also has the functions of regulating intestinal flora, enhancing immunity and resisting tumors, and is frequently used in the field of food processing.
Disclosure of Invention
The invention aims to provide an extraction method of pentosan, which has high extraction rate of pentosan and can ensure the content of part of vitamins in the processing process.
A method for extracting pentosan comprises the following steps: (1) Grinding wheat into flour, adding water, homogenizing and heating at 30-40 deg.C for 10-12 hr, and separating by three-phase turbine centrifuge to obtain coarse pentosan liquid, wheat gluten liquid and rest starch milk; curing the wheat gluten liquid, dehydrating and drying to obtain a finished wheat gluten product;
(2) Filtering the rest starch milk with 30-40% water, mixing with the crude pentosan solution, fermenting to obtain alcohol product and mash, adding protease, cellulase and alpha-amylase into the mash, adding modified nano porous silica, continuing fermenting at 40-50 deg.C for 3-4h, filtering starch, protein and fat by microfiltration, filtering organic acid, amino acid and salt by secondary microfiltration, and dehydrating to obtain pentosan fine product. Further, the modified porous silica is prepared by the following steps: adding ethanol solution into nanometer porous silicon dioxide, performing ultrasonic treatment at 400-500W for 20-30min, cleaning with clear water for 1-2min, adding nanometer powdered vitamin, adding collagen, stirring at 45-50 deg.C, oven drying, and grinding to obtain nanometer powder, i.e. modified porous silicon dioxide.
Further, the mass volume ratio of the flour to the water in the step (1) is 1-2kg; the mass-volume ratio of the mash to the protease, the cellulase, the alpha-amylase and the modified nano-porous silica is (20-30L).
Further, in the preparation step of the modified porous silica: the mass concentration of the ethanol solution is 65-75%; the mass ratio of the nano-porous silicon dioxide, the vitamins and the collagen is as follows: 1-2mg.
A system for an extraction method for preparing pentosan comprises a flour milling machine, wherein the flour milling machine is communicated with a homogenizer, the homogenizer is communicated with a three-phase turbo-centrifuge, the three-phase turbo-centrifuge comprises a coarse pentosan outlet, a wheat gluten outlet and other starch outlets, the wheat gluten outlet is connected with a first ripening tank, the first ripening tank is connected with a dehydrator, the dehydrator is connected with a dryer, and the dryer is connected with a packaging system; the other starch outlets are connected with a filter, the filter is communicated with a second aging tank, the inlet of the second aging tank is also connected with a coarse pentosan outlet, the outlet of the second aging tank is communicated with a fermentation microfiltration device, the first outlet of the fermentation microfiltration device is connected with an alcohol collecting tank, the second outlet of the fermentation microfiltration device is communicated with a dryer, and the dryer is connected with a finished product packaging system.
Further, the fermentation microfiltration device comprises a support, the support comprises two vertical plates which are arranged oppositely, the lower ends of the vertical plates are connected through a transverse plate, the surfaces of the vertical plates are perpendicular to the surface of the transverse plate, a rotatable fermentation tank is arranged between the two vertical plates, the fermentation tank is cylindrical and hollow, the side wall of the fermentation tank is in sliding connection with the two vertical plates through a connecting rod, one end of the connecting rod is connected with the side wall of the fermentation tank, the other end of the connecting rod is connected with the vertical plates through a bearing to form a freely rotating structure of the fermentation tank between the two vertical plates, a fermentation liquid inlet and an air inlet are arranged on the fermentation tank, openable sealing covers are arranged on the fermentation liquid inlet and the air inlet, a heater is arranged in the fermentation tank, the fermentation tank is of a cylindrical cavity structure, a cleaning opening is arranged on the side wall of the fermentation tank, but be equipped with open closed sealing plug on the washing mouth, the fermentation cylinder passes through communicating pipe intercommunication collection liquid jar, it includes first drum to collect liquid jar, the second drum is established to first drum endotheca, the interval has between first drum and the second drum, the upper shed parallel and level of first drum and second drum just is connected through the upper plate, first drum under shed is less than the under shed of second drum, the under shed of first drum passes through the hypoplastron and seals, communicating pipe upper end opening and fermentation cylinder intercommunication, be equipped with the first opening of several on the upper plate, first opening is towards the fermentation cylinder direction, the second opening is opposite with the direction of first opening, the middle section lateral wall and the second drum inside wall fixed connection of communicating pipe, communicating pipe lower extreme opening is located the interval position between first drum and the second drum.
Further, communicating pipe include upper segment pipe, well section pipe and hypomere pipe, the length direction and the middle section pipe length direction of upper segment pipe are the obtuse angle in the coplanar, this obtuse angle is towards the place direction of first drum, be equipped with the solenoid valve on the upper segment pipe, the length direction and the middle section pipe length direction of hypomere pipe are the obtuse angle in the coplanar, this obtuse angle is towards the place direction of first drum, middle section pipe mid portion is the rubber tube, rubber tube upper end opening and lower extreme opening pass through screw thread and middle section union coupling, the filter screen device can be dismantled to the rubber tube endotheca.
Further, the rubber tube inside wall be equipped with the several plush copper, the plush copper is along the even equidistant range of length direction of rubber tube, the filter screen device includes the circular filter of several, the circular axial plane border of two adjacent filters is connected through two spinal branch vaulting poles, the filter includes the ring, the ring internal fixation covers filtration membrane, when the filter screen device inserted the rubber tube, the plush copper was located between two adjacent filters.
Furthermore, the lateral wall of first drum set up the detachable condensation bag through the primary and secondary subsides, be equipped with primary and secondary subsides on this first drum lateral wall and collude the face, the condensation bag is equipped with the cotton face that uses with this colludes the face cooperation.
Furthermore, the pore diameters of the filter membranes are respectively 0.012-0.015 mu m.
In the system and the equipment manufacturing process, a fermentation tank and a liquid collection tank are arranged in a fermentation microfiltration device to form a rotatable structure, alcohol and mash can be separated out by rotating the position of the fermentation tank and the liquid collection tank, a detachable communicating pipe structure is arranged, a filter screen can be replaced at any time, a cleaning opening is also arranged, filtered residues can be discharged by gravity to facilitate cleaning, the filtering speed can be accelerated by the self gravity of materials in the filtering process, the filtering effect is accelerated, the fermentation tank can be rotated to enable the fermentation tank to be uniformly mixed in the fermentation process, and the pentosan extraction rate is also increased after the equipment is used.
Drawings
FIG. 1 is a system flow diagram of the present invention;
FIG. 2 is a schematic structural diagram of a fermentation microfiltration device;
FIG. 3 is a schematic structural view of a liquid collection tank;
FIG. 4 is a view showing a connection relationship between the communication pipe and the second cylinder;
FIG. 5 is a diagram showing the position relationship between the filter plate and the support rod;
FIG. 6 is a schematic view of a filter plate;
fig. 7 is a schematic structural view of the first cylinder and the condensation bag.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1:
the extraction method of pentosan comprises the following steps: (1) Grinding wheat into flour, adding water, homogenizing and heating at 30 deg.C for 10 hr, separating with three-phase turbine centrifuge to obtain coarse pentosan liquid, wheat gluten liquid and starch milk; and curing the wheat gluten liquid, dehydrating and drying to obtain a finished wheat gluten product.
(2) Filtering the rest starch milk with 30% water, mixing with the crude pentosan solution, fermenting to obtain alcohol product and mash, adding protease, cellulase and alpha-amylase into the mash, adding modified nano porous silicon dioxide, fermenting at 50 deg.C for 3 hr, filtering starch, protein and fat by microfiltration, filtering organic acid, amino acid and salt by secondary microfiltration, and dehydrating to obtain pentosan fine product.
The modified porous silica is prepared by the following steps: adding ethanol solution into nanometer porous silicon dioxide, performing ultrasonic treatment at 200W for 20min, cleaning with clear water for 1min, and adding nanometer powdered vitamin B 2 Then adding collagen, stirring uniformly at 45 ℃, drying and grinding to obtain nanoscale powder, namely the modified porous silicon dioxide.
The mass volume ratio of the flour to the water in the step (1) is 1kg; the mass-volume ratio of the mash to the protease, the cellulase, the alpha-amylase and the modified nano-porous silica is 20L, 10mg.
The preparation steps of the modified porous silica are as follows: the mass concentration of the ethanol solution is 65-75%; the nano porous silicon dioxide and the vitamin B 2 The mass ratio of the collagen to the collagen is as follows: 1mg.
Example 2:
a method for extracting pentosan comprises the following steps: (1) Grinding wheat into flour, adding water, homogenizing and heating at 35 deg.C for 11 hr, separating with three-phase turbine centrifuge to obtain coarse pentosan liquid, wheat gluten liquid and rest starch milk; and curing the wheat gluten liquid, dehydrating and drying to obtain a finished wheat gluten product.
(2) Filtering the rest starch milk with 35% of water, mixing with the crude pentosan solution, fermenting to obtain alcohol finished product and mash, adding protease, cellulase and alpha-amylase into the mash, adding modified nano porous silica, continuing fermenting at 40 deg.C for 3.5h, filtering starch, protein and fat by microfiltration, filtering organic acid, amino acid and salt by secondary microfiltration, and dehydrating to obtain pentosan fine product.
The modified porous silica is prepared by the following steps: adding ethanol solution into nanometer porous silicon dioxide, performing ultrasonic treatment at 450W for 25min, cleaning with clear water for 1.5min, adding nanometer powdery vitamin calcium, adding collagen, stirring at 48 deg.C, oven drying, and grinding to obtain nanometer powder, i.e. modified porous silicon dioxide.
In the step (1), the mass volume ratio of the flour to the water is 1.5kg; the mass volume ratio of the mash to the protein enzyme, the cellulase, the alpha-amylase and the modified nano-porous silica is 25L, 18mg.
The preparation steps of the modified porous silica are as follows: the mass concentration of the ethanol solution is 70 percent; the mass ratio of the nano-porous silicon dioxide, the calcium and the collagen is as follows: 1.5mg.
Example 3:
a method for extracting pentosan comprises the following steps: (1) Grinding wheat into flour, adding water, homogenizing and heating at 40 deg.C for 12 hr, separating with three-phase turbine centrifuge to obtain coarse pentosan liquid, wheat gluten liquid and rest starch milk; and curing the wheat gluten liquid, dehydrating and drying to obtain a finished wheat gluten product.
(2) Filtering the rest starch milk with 40% of water, mixing with the crude pentosan solution, fermenting to obtain alcohol finished product and mash, adding protease, cellulase and alpha-amylase into the mash, adding modified nano porous silicon dioxide, continuing fermenting for 4h at 45 ℃, filtering starch, protein and fat by using a microfiltration mode, filtering organic acid, amino acid and salt by using a secondary microfiltration mode, and dehydrating to obtain pentosan fine product.
The modified porous silica is prepared by the following steps: adding ethanol solution into nanometer porous silicon dioxide, performing ultrasonic treatment at 500W for 30min, cleaning with clear water for 2min, and adding nanometer powdered vitamin B 2 Then adding collagen, stirring uniformly at 50 ℃, drying and grinding to obtain nanoscale powder, namely the modified porous silicon dioxide.
The mass volume ratio of the flour to the water in the step (1) is 2kg; the mass-volume ratio of the mash to the protease, the cellulase, the alpha-amylase and the modified nano-porous silica is 30L, 20mg.
The preparation steps of the modified porous silica are as follows: the mass concentration of the ethanol solution is 75 percent; the nano porous silicon dioxide and the vitamin B 2 The mass ratio of the collagen to the collagen is as follows: 2mg.
The system for the extraction method for preparing pentosan, as shown in fig. 1-7, comprises a flour mill 1, wherein the flour mill 1 is communicated with a homogenizer 2, the homogenizer 2 is communicated with a three-phase turbo-centrifuge 3, the three-phase turbo-centrifuge 3 comprises a coarse pentosan outlet 301, a vital gluten outlet 302 and other starch outlets 303, the vital gluten outlet 302 is connected with a first curing tank 4, the first curing tank 4 is connected with a dehydrator 5, the dehydrator 5 is connected with a dryer 6, and the dryer 6 is connected with a packaging system 7; the rest starch outlets 303 are connected with a filter 8, the filter 8 is communicated with a second curing tank 9, the inlet 901 of the second curing tank is also connected with a crude pentosan outlet 301, the outlet 902 of the second curing tank is communicated with a fermentation microfiltration device 10, the first outlet of the fermentation microfiltration device 10 is connected with an alcohol collecting tank 11, the second outlet of the fermentation microfiltration device 10 is communicated with a dryer 12, and the dryer 12 is connected with a finished product packaging system 7. The fermentation microfiltration device 10 comprises a support 101, the support 101 comprises two opposite vertical plates 1011, the lower ends of the vertical plates 1011 are connected through a transverse plate 1012, the plate surfaces of the vertical plates 1011 are perpendicular to the plate surface of the transverse plate 1012, a rotatable fermentation tank 102 is arranged between the two vertical plates 1011, the fermentation tank 102 is cylindrical and hollow inside, when in use, the circular axial surface of the fermentation tank 102 faces towards the transverse plate or the upper direction, the side wall of the fermentation tank 102 is connected with the two vertical plates 1011 in a sliding way through a connecting rod, one end of the connecting rod is connected with the side wall of the fermentation tank 102, the other end of the connecting rod is connected with the vertical plates 1011 through a bearing 13 to form a structure that the fermentation tank 102 is located between the two vertical plates 1011 and can rotate freely, the fermentation tank 102 is provided with a fermentation liquor inlet 103 and an air inlet 105, the fermentation liquor inlet 103 and the air inlet 105 are both provided with openable sealing covers 105, a cylindrical heater 106 is arranged in the fermentation tank 102, the fermentation tank 102 is of a cavity structure, the side wall of the fermentation tank 102 is provided with a cleaning opening 107, the cleaning opening 107 is provided with an openable sealing plug 108, the fermentation tank 102 is communicated with a liquid collecting tank 109 through a communicating pipe 110, the liquid collecting tank 109 comprises a first cylinder 1091, a second cylinder 1092 is sleeved in the first cylinder 1091, a space is arranged between the first cylinder 1091 and the second cylinder 1092, the upper openings of the first cylinder 1091 and the second cylinder 1092 are flush and are connected through an upper plate 1093, the lower opening of the first cylinder 1091 is lower than the lower opening of the second cylinder 1092, the cylinder height of the first cylinder 1091 is larger than that of the second cylinder 1092, the lower opening of the first cylinder 1091 is sealed through a lower plate, the upper end opening of the communicating pipe 110 is communicated with the fermentation tank 102, the upper plate 1093 is provided with four first openings 1094, the first openings 1094 face the fermentation tank 102, the second openings 1905 are opposite to the first openings 1094, the side wall of the middle section of the communicating pipe 110 is fixedly connected with the inner side wall of the second cylinder 1092, the lower end opening of the communication pipe 110 is located at a spaced position between the first cylinder 1091 and the second cylinder 1092. The upper portion and the lower portion of the middle section of the communication pipe 110 are fixed on the inner side wall of the second cylinder 1092 through fixing clamping seats respectively, the communication pipe 110 comprises an upper section pipe 1101, a middle section pipe 1102 and a lower section pipe 1103, the length direction of the upper section pipe 1101 and the length direction of the middle section pipe 1102 form an obtuse angle in the same plane, the obtuse angle faces the direction of the first cylinder 1091, an electromagnetic valve 1105 is arranged on the upper section pipe 1101, the length direction of the lower section pipe 1103 and the length direction of the middle section pipe 1102 form an obtuse angle in the same plane, the obtuse angle faces the direction of the first cylinder 1091, the middle section pipe 1102 is a rubber pipe 1104, an upper end opening and a lower end opening of the rubber pipe 1104 are connected with the middle section pipe 1102 through threads, and the detachable filter screen device 1106 is sleeved in the rubber pipe 1104.
The inside wall of rubber tube 1104 be equipped with seven plush copper 1107, plush copper 1107 is along the even equidistant range of rubber tube 1104's length direction, filter screen device 1106 includes eight circular filter 1108, the circular axial face border of two adjacent filter 1108 is connected through two spinal branch vaulting poles 1109, eight filter top-down are along the even equidistant range of vertical direction, filter 1108 includes ring 1110, ring 1110 internal fixation covers filtration membrane 1111, when filter screen device 1106 inserts the rubber cylinder, plush copper 1107 is located between two adjacent filter 1108. The outer side wall of the first cylinder 1091 is provided with a detachable condensation bag 1112 through a primary-secondary paste, the outer side wall of the first cylinder 1091 is provided with a primary-secondary paste hook surface, and the condensation bag 1112 is provided with a cotton surface matched with the hook surface for use.
When the wheat gluten processing device is used, wheat is placed into a flour mill 1 to be ground into flour, water is added, the flour is fed into a homogenizer 2 through a pipeline, the flour is homogenized and heated for 10-12 hours at the temperature of 35 ℃ in the homogenizer 2, then the flour comes out of the homogenizer 2 and enters a three-phase turbo centrifuge 3, the homogenized material is separated by the three-phase turbo centrifuge 3 and is respectively divided into coarse pentosan liquid, wheat gluten liquid and other starch milk, the coarse pentosan liquid, the wheat gluten liquid and the other starch milk flow out of a coarse pentosan outlet 301, a wheat gluten outlet 302 and other starch outlets 303, the wheat gluten outlet 302 is connected with a first curing tank 4 for curing, the cured material enters a dehydrator 5 to dehydrate the wheat gluten liquid, the dehydrator 5 is connected with a dryer 6, and the dehydrated wheat gluten enters the dryer 6 to be dried and then enters a packaging system 7. And the rest starch milk flows out from the rest starch outlet 303, is filtered by the filter 8, is partially dehydrated, enters the second curing tank 9, is subjected to curing in the second curing tank 9, enters the fermentation microfiltration device 10 after being cured, is fermented, is separated into alcohol and mash, and enters the alcohol collecting tank 11 from the first outlet of the fermentation microfiltration device 10 to obtain an alcohol finished product, and enters the dryer 12 from the second outlet of the fermentation microfiltration device 10, and is dried and then enters the finished product packaging system 7.
When the fermentation microfiltration device 10 is used, the electromagnetic valve 1105 is closed, mature liquid from the second maturation tank 9 enters from the fermentation liquid inlet 103 of the fermentation tank 102, saccharomyces cerevisiae is put in, the air inlet 105 is opened, sterile gas is conveyed, the heater 106 is opened, the heater 106 is heated at constant temperature, after saccharomyces cerevisiae is bred, the air inlet 105 and the fermentation liquid inlet 103 are closed, the liquid collection tank 109 is turned over to the upper side of the fermentation tank 102, the electromagnetic valve 1105 is opened, during fermentation, alcohol is volatile and enters the liquid collection tank 109 along the communicating pipe 110, the gasified alcohol is liquefied again by the condensation bag 1112, comes out from the first outlet of the fermentation microfiltration device 10 and enters the alcohol collection tank 11 to obtain an alcohol finished product, after fermentation, alcohol separation is finished, the communicating pipe 110 is detached, when the rubber cylinder is inserted into the filter screen device 1106, the convex head is positioned between two adjacent filter plates 1108, the middle section of the communicating pipe 110 is communicated with the upper section pipe and the lower section pipe of the communicating pipe 1101 again, after fermentation, protease, the filter screen 1103 is added into the filtrate of the filter screen 1106, the filtrate of the second fermentation tank 110, the filtrate is dried by gravity, the second filtration device 102, the second fermentation tank 102, and the second fermentation tank 102 is obtained. The pore diameters of the filter membranes are respectively 0.012-0.015 mu m.
Test example 1:
comparative group 1 was conducted to calculate the extraction rate of pentosan produced by using nano-silica without adding it as starch slurry, adding protease, decomposing amylase, and microfiltering.
In the case of the comparison group 2, the pentosan is used as starch slurry without adding nano silicon dioxide, saccharomycetes are added, and after protease and amylase decomposition, microfiltration is carried out to obtain the extraction rate of the pentosan.
The extraction rates obtained by the methods of the invention in example 1 and example 2 were used, and the nutrient retention was measured, and the results are as follows, wherein the pentosan extraction rate is calculated as the percentage (mass of pentosan after extraction/total mass of starch) x 100% calculated as the amount of 100g of starch, and the vitamin measurement is performed with 1000g of starch as the test amount:
pentosan extraction rate Vitamin B 2 Calcium carbonate
Example 1 41.3％ 7.321mg 4.9mg
Example 2 43.4％ 3.126mg 9.6mg
Comparative group 1 21.5％ 0.032mg 0.03mg
Comparative group 2 25.2％ 0.126mg 0.23mg
From the above, under the condition of adding silicon dioxide, part of vitamins in the pentosan prepared by the method can be reserved, the extraction rate of the pentosan is greatly improved, and the vitamin content is also greatly improved. In contrast group, the vitamins of macromolecules after filtration are also filtered, so that the content of vitamins in the finished product can not be ensured. Test example 2:
using the method of the invention, and simultaneously using the apparatus of the invention, the extraction yields obtained were used as a comparison:
extraction ratio of pentosan Vitamin B 2 Calcium carbonate
Example 1 41.3％ 7.321mg 4.9mg
Example 2 43.4％ 3.126mg 9.6mg
Example 3 52.5％ 9.849mg 5.7mg
From the above, the extraction rate of pentosan and the content of vitamins are greatly improved by using the equipment of the invention.

Claims (9)

1. The extraction method of pentosan is characterized by comprising the following steps: (1) Grinding wheat into flour, adding water, homogenizing at 30-40 deg.C for 10-12h, separating by three-phase turbine centrifuge to obtain coarse pentosan liquid, wheat gluten liquid and rest starch milk; curing the wheat gluten liquid, dehydrating and drying to obtain a finished wheat gluten product; (2) Filtering the rest starch milk with 30-40% water, mixing with the crude pentosan solution, fermenting to obtain alcohol product and mash, adding protease, cellulase and alpha-amylase into the mash, adding modified nano porous silica, continuing fermenting at 40-50 deg.C for 3-4h, filtering starch, protein and fat by microfiltration, filtering organic acid and salt by secondary microfiltration, and dehydrating to obtain pentosan fine product; the modified porous silica is prepared by the following steps: adding ethanol solution into nanometer porous silicon dioxide, performing ultrasonic treatment at 400-500W for 20-30min, cleaning with clear water for 1-2min, adding nanometer powdered vitamin, adding collagen, stirring at 45-50 deg.C, oven drying, and grinding to obtain nanometer powder, i.e. modified porous silicon dioxide.
2. The extraction method of pentosan according to claim 1, wherein the mass volume ratio of flour to water in step (1) is 1-2kg; the mass volume ratio of the mash to the protease, the cellulase, the alpha-amylase and the modified nano-porous silica is 20-30L, 10-20mg.
3. The method for extracting pentosan according to claim 1, wherein said modified porous silica is prepared by the steps of: the mass concentration of the ethanol solution is 65-75%; the mass ratio of the nano-porous silicon dioxide, the vitamins and the collagen is as follows: 1-2mg.
4. A preparation system using the extraction method of pentosan of claim 1, which comprises a flour milling machine, wherein the flour milling machine is communicated with a homogenizer, the homogenizer is communicated with a three-phase turbo-centrifuge, the three-phase turbo-centrifuge comprises a crude pentosan outlet, a wheat gluten outlet and other starch outlets, the wheat gluten outlet is connected with a first ripening tank, the first ripening tank is connected with a dehydrator, the dehydrator is connected with a dryer, and the dryer is connected with a packaging system; the other starch outlets are connected with a filter, the filter is communicated with a second aging tank, the inlet of the second aging tank is also connected with a coarse pentosan outlet, the outlet of the second aging tank is communicated with a fermentation microfiltration device, the first outlet of the fermentation microfiltration device is connected with an alcohol collecting tank, the second outlet of the fermentation microfiltration device is communicated with a dryer, and the dryer is connected with a finished product packaging system.
5. The system of claim 4, wherein the fermentation microfiltration device comprises a support, the support comprises two opposite vertical plates, the lower ends of the vertical plates are connected through a transverse plate, the surfaces of the vertical plates are perpendicular to the surface of the transverse plate, a rotatable fermentation tank is arranged between the two vertical plates, the fermentation tank is cylindrical and hollow, the side wall of the fermentation tank is slidably connected with the two vertical plates through a connecting rod, one end of the connecting rod is connected with the side wall of the fermentation tank, the other end of the connecting rod is connected with the vertical plates through a bearing to form a freely rotating structure of the fermentation tank between the two vertical plates, the fermentation tank is provided with a fermentation liquid inlet and an air inlet, the fermentation liquid inlet and the air inlet are both provided with openable sealing covers, a heater is arranged in the fermentation tank, the fermentation tank is cylindrical cavity structure, the side wall of the fermentation tank is provided with a cleaning opening, but be equipped with open closed sealing plug on the washing mouth, the fermentation cylinder passes through communicating pipe intercommunication collection liquid jar, it includes first drum to collect liquid jar, the second drum is established to first drum endotheca, the interval has between first drum and the second drum, the upper shed parallel and level of first drum and second drum just is connected through the upper plate, first drum under shed is less than the under shed of second drum, the under shed of first drum passes through the hypoplastron and seals, communicating pipe upper end opening and fermentation cylinder intercommunication, be equipped with the first opening of several on the upper plate, first opening is towards the fermentation cylinder direction, the second opening is opposite with the direction of first opening, the middle section lateral wall and the second drum inside wall fixed connection of communicating pipe, communicating pipe lower extreme opening is located the interval position between first drum and the second drum.
6. The system according to claim 5, wherein the communicating pipe comprises an upper pipe, a middle pipe and a lower pipe, the length direction of the upper pipe and the length direction of the middle pipe form an obtuse angle in the same plane, the obtuse angle faces the direction of the first cylinder, the upper pipe is provided with an electromagnetic valve, the length direction of the lower pipe and the length direction of the middle pipe form an obtuse angle in the same plane, the obtuse angle faces the direction of the first cylinder, the middle part of the middle pipe is a rubber pipe, an upper end opening and a lower end opening of the rubber pipe are connected with the middle pipe through threads, and a detachable filter screen device is sleeved in the rubber pipe.
7. The system according to claim 6, wherein the inner side wall of the rubber tube is provided with a plurality of raised heads, the raised heads are uniformly and equally spaced along the length direction of the rubber tube, the filter screen device comprises a plurality of circular filter screens, the circular axial surface edges of two adjacent filter screens are connected through two support rods, the filter screens comprise circular rings, the circular rings are fixedly covered with the filter screens, and when the filter screen device is inserted into the rubber tube, the raised heads are positioned between the two adjacent filter screens.
8. The system according to claim 7, wherein the outer side wall of the first cylinder is provided with a detachable condensation bag through a snap sticker, the outer side wall of the first cylinder is provided with a snap sticker hook surface, and the condensation bag is provided with a cotton surface matched with the hook surface for use.
9. The system of claim 8, wherein the pore diameters of the filtration membranes are respectively 0.012-0.015 μm.
CN202210670285.4A 2022-06-14 2022-06-14 Extraction method of pentosan Active CN114933664B (en)

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