CN114916463A - Experimental animals anxiety trigeminy composite behavior detection device - Google Patents
Experimental animals anxiety trigeminy composite behavior detection device Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
- A01K15/027—Exercising equipment, e.g. tread mills, carousels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Abstract
The utility model provides a compound action detection device of experimental animals anxiety trigeminy, relates to biological correlation industry technical field, including base, spacious field case, overhead maze, light and dark case and support, fixed surface is connected with the support on the base, and the top of support from left to right in proper order fixedly connected with spacious field case, overhead maze and light and dark case, spacious field case, overhead maze and light and dark case interconnect in proper order to respectively through first passageway, second passageway intercommunication, the support is hidden respectively in the below of spacious field case, overhead maze, light and dark case. The invention can synchronously measure three different behavior experiments, can minimize the influence of individual emotion fluctuation of experimental animals, avoids the defects of anxiety behavior reduction and lack of referential measured data caused by separate measurement among different devices, and simultaneously reduces the animal cost and the time cost. Experimental data prove that the effect of the invention is superior to that of the traditional ethology test, and the invention can be popularized as a test mode for replacing the original ethology test.
Description
Technical Field
The invention relates to the technical field of bio-related industries, in particular to a triple-combination behavior detection device for anxiety of experimental animals.
Background
The open field experiment is also called an open box experiment, is a classical ethology method for evaluating the motor ability and emotional state (anxiety, depression and the like) of rodent experimental animals, and is also a method for evaluating the autonomous behavior of the experimental animals in a new and different environment and exploring behavior and tensity. The method is widely applied to basic researches of rehabilitation medicine, geriatric medicine, psychiatry, neuroscience, pharmacology and the like. The occurrence frequency and the duration of certain behaviors of the experimental animal in the novel environment are used for reflecting the autonomous behavior and the exploration behavior of the experimental animal in the strange environment, and the quantity of excrement is used for reflecting the tension. For example, common observation indicators include residence time and times of animals in the central area, standing times, dressing times, movement speed, movement distance, rest time, and the like.
The elevated plus maze is used for inspecting the anxiety state of animals by utilizing the exploration characteristics of the animals to the new and different environments and the fear of high-suspension open arms to form contradictory conflict behaviors. The elevated plus maze has a pair of open arms and a pair of closed arms, rodents tend to move in the closed arms due to darkness but move in the open arms due to curiosity and exploratory property, and when facing a novel stimulus, the animals simultaneously generate an exploratory impulse and fear, which cause conflicting exploration and avoidance behaviors, thereby generating anxiety psychology. The anxiolytic can obviously increase the times and time for entering the open arm, and the cross maze is higher from the ground, which is equivalent to a human standing on the cliff, so that the experimental subject generates fear and uneasy mind. The elevated plus maze is widely applied to the fields of scientific research and computer-aided education of a plurality of subjects such as new drug development screening/evaluation, pharmacology, toxicology, preventive medicine, neurobiology, animal psychology, behavior biology and the like, and is a classic experiment for developing behavioral research, particularly anxiety and depression research.
The light and dark box experiment is a widely used behavior detection method, is used for screening and evaluating the activity of anxiolytic drugs, and can also be used for researching the pathological mechanism of anxiety disorder. Because the dark box experiment has the advantages of simple operation and no need of training animals in advance, the method becomes a classical method for evaluating the behavior of the rodent state anxiety and the idiosyncratic anxiety. The light and dark box utilizes the fact that rodents like to explore a novel environment, but is forced to retreat due to the fact that the rodents dislike the bright light in a bright room, so that a contradiction conflict state is formed, and the number of times of box penetrating is reduced. Anxiolytic drugs can significantly increase the number of craters worn by the rats and the time in the light room.
The most common solution is to use a series of different experimental tests, however as the tests increase the accuracy of the tests necessarily decreases — an experiment in one test may have an impact (usually inhibitory behavior) in subsequent tests, and repeated tests may also create an environmental aversion. Ultimately results in: mice that exhibit high levels of anxiety-like behavior in one facility exhibit low anxiety behavior in a different facility, and the experimenter will never be able to estimate or eliminate this inconsistent factor.
Disclosure of Invention
The invention provides a triple combined behavior detection device for anxiety of experimental animals, which can synchronously measure three different behavior experiments, minimize the influence of individual emotion fluctuation, avoid the defects of anxiety behavior reduction and measurement data lack of referential property caused by separate measurement among different devices, and simultaneously reduce the cost of animals and the time cost. Experimental data prove that the effect of the invention is superior to that of the traditional behavioristics test, and the invention can be popularized as a test mode for replacing the original behavioristics test.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the utility model provides a compound action detection device of experimental animals anxiety trigeminy, includes base, spacious field case, overhead maze, light and dark case and support, base upper surface fixedly connected with support, the top of support from left to right in proper order fixedly connected with spacious field case, overhead maze and light and dark case, spacious field case, overhead maze and light and dark case interconnect in proper order to communicate through first passageway, second passageway respectively, the support hide the below at spacious field case, overhead maze, light and dark case respectively.
Preferably, the open-field box is of a box body structure with an open top end, and the upper surface of the box bottom is divided into 1 central area and 8 peripheral areas by horizontal and vertical crossed lines; the light and dark box sequentially comprises a dark box and a light box which are mutually attached and fixedly connected from left to right, the dark box and the light box are both of a closed structure, the dark box is communicated with the light box through a third channel, the third channel penetrates through the bottom of the side wall where the dark box is connected with the light box, and the bottom of the side wall of the dark box, which faces one side of the elevated labyrinth, is provided with a second channel and is communicated with the elevated labyrinth through the second channel; spacious field case lateral wall bottom towards overhead maze one side be equipped with first passageway to communicate through first passageway and overhead maze.
Preferably, the inner wall of the dark box is coated with black and is internally provided with a red illuminating lamp, and the inner wall of the bright box is coated with white and is internally provided with a white illuminating lamp.
Preferably, the elevated labyrinth is the elevated labyrinth of cross, include along the first cantilever that left and right sides direction set up and along the fore-and-aft direction setting and constitute the second cantilever of cruciform structure with first cantilever, the cross-section of first cantilever be fluted body structure, the cross-section of second cantilever be square structure, be equipped with the platform in first cantilever and second cantilever cross connection's central point position.
Preferably, the elevated labyrinth is a horizontally arranged circular labyrinth, the left end and the right end of the circular labyrinth are respectively provided with a first groove section, a first platform section is respectively arranged between the opposite ends of the 2 first groove sections, and the 2 first groove sections are respectively communicated with the open field box and the light and dark box.
Preferably, the elevated labyrinth is a straight-line labyrinth, which comprises second groove sections at two ends and a second platform section connected between the 2 second groove sections, wherein the 2 second groove sections are respectively communicated with the open field box and the light and dark box.
Preferably, the base is arranged between the base and the bracket; the bracket is respectively arranged between the open field box, the light and dark box and the elevated labyrinth; the elevated labyrinth is detachably and fixedly connected with the light and dark box and the open field box through bolts respectively.
The experimental animal anxiety triple composite behavior detection device has the beneficial effects that:
the invention can synchronously measure three different behavior experiments, can minimize the influence of individual emotion fluctuation of experimental animals, avoids the defects of anxiety behavior reduction and measurement data lack of referential caused by separate measurement among different devices, and simultaneously reduces the cost of animals and the time cost. Experimental data prove that the effect of the invention is superior to that of the traditional behavioristics test, and the invention can be popularized as a test mode for replacing the original behavioristics test.
Drawings
FIG. 1: the invention is a schematic front structure;
FIG. 2 is a schematic diagram: the invention is a schematic view of a top-down structure;
FIG. 3 is a partial cross-sectional view of the invention in a top view;
FIG. 4 is a schematic top view of the circular elevated labyrinth of the present invention;
FIG. 5 is a schematic top view of the present invention with a straight elevated labyrinth;
FIG. 6 is a comparison graph of the total course experimental data in the open field experiment of the present invention and the conventional open field box;
FIG. 7 is a comparison graph of the distance data of the central area in the open field experiment between the present invention and the conventional open field box;
FIG. 8 is a comparison graph of central region entry times data in an open field experiment of the present invention and a conventional open field chamber;
FIG. 9 is a graph comparing the percentage of open arm access times in the present invention versus a conventional elevated plus maze experiment;
FIG. 10 is a graph comparing the percent open arm dwell time data in the present invention versus a conventional elevated plus maze experiment;
FIG. 11 is a comparison graph of distance data in bright area between the present invention and the conventional light and dark box experiment;
FIG. 12 is a graph comparing the data of the residence time in the bright area in the experiment of the present invention and the conventional light and dark boxes;
1. a base; 2. a support; 3. an open field box; 3-1, central zone; 3-2, peripheral zone; 4. a cross-shaped elevated maze; 4-1, a first cantilever; 4-2, a second cantilever; 4-3, a platform; 5. a dark box; 6. the box is exposed; 7. a first channel; 8. a second channel; 9. a third channel; 10. a first groove section; 11. a first platform section; 12. a second groove segment; 13. a second platform section.
Detailed Description
Examples 1,
The utility model provides a compound action detection device of experimental animals anxiety trigeminy, is shown as figure 1-3, including base 1, spacious field case 3, overhead maze, light and dark case and support 2, 1 upper surface fixedly connected with support 2 on the base, the top of support 2 from left to right fixedly connected with spacious field case 3, overhead maze and light and dark case in proper order, spacious field case 3, overhead maze and light and dark case interconnect in proper order to communicate through first passageway 7, second passageway 8 respectively, support 2 hide respectively in the below of spacious field case 3, overhead maze, light and dark case, avoid experimental animals climbing support to escape, make the unsettled assigned height of overhead maze (the support only is the signal in the picture simultaneously, the support can be for satisfying all kinds of supports of above-mentioned condition).
As shown in fig. 1-3, the open-field box 3 is a box structure with an open top, and the upper surface of the box bottom is divided into 1 central area 3-1 and 8 peripheral areas 3-2 by horizontal and vertical crossed lines; the light and dark box sequentially comprises a dark box 5 and a light box 6 which are mutually attached and fixedly connected from left to right, the dark box and the light box are both of a closed structure, the dark box is communicated with the light box through a third channel 9, the third channel 9 penetrates through the bottom of the side wall of the dark box connected with the light box, and the bottom of the side wall of the dark box, which faces one side of the elevated labyrinth, is provided with a second channel 8 and is communicated with the elevated labyrinth through the second channel 8; spacious field case 3 lateral wall bottom towards overhead maze one side be equipped with first passageway 7 to communicate through first passageway 7 and overhead maze.
As shown in fig. 1-3, the inner wall of the dark box 5 is coated with black and is internally provided with a red illuminating lamp, and the inner wall of the bright box 6 is coated with white and is internally provided with a white illuminating lamp.
The embodiment is the most basic implementation mode of the invention, and the anxiety triple experiment can be carried out by combining the three behavior experiment devices together, thereby overcoming the defects of reduced anxiety behavior of experimental animals and unreliable experimental data caused by separately carrying out experiments in the traditional experiment method.
Examples 2,
On the basis of embodiment 1, the present embodiment is further improved as follows:
as shown in fig. 1-3, the elevated maze is a cross elevated maze, comprising a first cantilever 4-1 arranged along the left-right direction and a second cantilever 4-2 arranged along the front-back direction and forming a cross structure with the first cantilever, the cross section of the first cantilever 4-1 is a groove structure, i.e. the experimental animal is in the environment with two closed sides, the cross section of the second cantilever is a square structure, i.e. the experimental animal on the upper surface of the second cantilever faces the environment with two open sides, a platform 4-3 is arranged at the center of the cross connection of the first cantilever and the second cantilever, and the experimental animal can select the exploration direction.
Examples 3,
On the basis of the embodiment 1, the embodiment is further improved as follows:
as shown in fig. 4, the elevated labyrinth is a horizontally arranged circular labyrinth, the left and right ends of the circular labyrinth are respectively provided with a first groove section 10, a first platform section 11 is respectively arranged between the opposite ends of the 2 first groove sections 10, and the 2 first groove sections 10 are respectively communicated with the open field box 3 and the light and dark boxes.
In this embodiment, the cross-sectional structure of the first groove section 10 is the same as that of the first cantilever 4-1; the cross-sectional structure of the first platform section 11 is the same as that of the second cantilever 4-2, and the experimental animal can selectively climb onto the first platform section 11 or stay in the first groove section 10.
Examples 4,
On the basis of the embodiment 1, the embodiment is further improved as follows:
as shown in fig. 5, the elevated labyrinth is a straight labyrinth, and includes second groove sections 12 located at two ends and a second platform section 13 connected between the 2 second groove sections 12, and the 2 second groove sections 12 are respectively communicated with the open field box and the bright and dark box.
In this embodiment, the cross-sectional structure of the second groove section 12 is the same as that of the first cantilever 4-1; the cross-sectional structure of the second platform section 13 is the same as that of the second cantilever 4-2, and the experimental animal can selectively climb onto the second platform section 13 or stay in the second groove section 12.
Examples 5,
On the basis of the above embodiment, the present embodiment is further modified as follows:
as shown in fig. 1-5, between the base 1 and the support 2; the bracket 2 is respectively arranged between the open field box, the light and dark box and the elevated labyrinth; the elevated labyrinth, the light and dark box and the open field box are respectively and fixedly connected through bolts, so that the elevated labyrinth is convenient to store and transport.
Experimental example:
evaluation of pharmacodynamics
1. Experimental animals:
144 male C57BL/6J mice (hereinafter referred to as C57) with a body mass of 20 + -2 g were adaptively bred for 7 days, and 12 mice were divided into 2 groups, the first group being: normal control group (36) and positive drug group (36). The second batch comprises the following steps: normal control group (36) and positive drug group (36).
144 male Kunming mice (KM for short) with the body mass of 20 +/-2 g are bred adaptively for 7 days, each group of 12 mice is divided into 2 batches according to a random block design method (according to the body mass and the total movement distance), and the first batch is divided into: normal control group (36) and positive drug group (36). The second batch comprises the following steps: normal control group (36) and positive drug group (36).
2. Administration:
purified water is filled into a normal group, the gastric lavage diazepam is filled into a positive drug group at a concentration of 2mg/kg, and the gastric lavage administration dosage of the mice is 10 ml/kg. Mice were gavaged daily at a fixed time in the morning (9:00) for 7 days. And (3) measuring the behavior of the mouse 1h after the last gavage by adopting an open field, an elevated plus maze, a light and dark box device and SuperMaze software of the mouse.
3. The experimental method comprises the following steps:
the first group of mouse control group and the positive drug group are divided into 3 groups respectively and are used for open field, elevated plus maze and light and dark box tests respectively.
The open field experiment is carried out in a traditional open field box, the bottom surface is equally divided into squares, the middle area is a central area, and the rest areas are peripheral areas. The bottom and the periphery of the open field box are all white. The experiment was performed in a quiet, low red environment, and the mice were initially placed in the center of an open field box and observed for activity within 5 min. Recording the total movement path, the movement path of the central area and the entering times of the central area of the mouse.
The elevated plus maze experiment is carried out by a traditional elevated plus maze experiment device, the head of a mouse faces to any direction of an open arm (equivalent to a second cantilever 4-2), the head of the mouse is placed in a central area (equivalent to a platform 4-3) crossed with the elevated plus maze, a video is recorded through a camera, the movement track of the mouse in 5min is recorded, and related behavior indexes are analyzed. The following relevant indexes are mainly recorded: time to open arm (OT), number of times to open arm (OE), time to close arm (CT) (equivalent to the first cantilever 4-1), and number of times to close arm (CE). OE% and OT% were calculated. The calculation method is as follows: OE% ═ OE/(OE + CE) × 100%, OT% ═ OT/(OT + CT) × 100%.
The light and dark box experiment is carried out in a traditional light and dark box experiment device, each mouse is placed in the center of a light area towards a partition wall at the beginning, and a door inserted between the partition walls is drawn away (which is equivalent to opening a third channel). One entry of the animal into the compartment with four paws is recorded. The computer records the movement track of the mouse within 5min, and the Total movement Distance (Total Distance), the bright area residence Time (Time in the light area), the bright area entry times (Entries in the light area) and the bright area Distance (Distance in the light area) are used as the indexes of movement and anxiety.
The second batch of mice was tested using a homemade anxiety triple compound behavior detection device.
The mice were placed in the open field center of the anxiety triple-combination behavior detection device and the behavior changes of the mice during the 15 minute trial were recorded.
The parameters of the self-made anxiety triple compound behavior detection device are as follows (a mouse experiment system is taken as an example): the open field box is 50cm long, 50cm wide and 50cm deep, the bottom surface of the open field box is equally divided into 9 squares, the middle area is the central area, the rest area is the peripheral area, and the bottom and the periphery of the open field box are all white. The elevated plus maze comprises 2 opposite open arms with the length of 35cm, the width of 6cm and the height of 20cm and 2 opposite closed arms with the length of 35cm, the width of 6cm and the height of 20cm, wherein the upper parts of the closed arms are open (the cross sections are of groove-shaped structures), the two sides of the open arms are open (the cross sections are of square structures), and a relatively wide part (namely a platform 4-3) with the length of 5cm and the width of 5cm is arranged in the center between the open arms and the closed arms. The light and dark box is composed of two compartments which are separated into two same sizes, the length is 25cm, the width is 25cm and the depth is 30cm, the bottom and the periphery of a light area are all white, the bottom of a dark area is white and the periphery of the dark area is black, the middle inserting door is 6.5cm long and 6.5cm wide, the light area is illuminated by white light, and the dark area is illuminated by red light.
The box body of the open field box, the elevated cross maze and the light and dark box are horizontally connected, and a channel with the length of 5cm and the width of 5cm is arranged at the joint for the mouse to freely pass through.
4. Statistical method
Data were analyzed using GraphPad Prism 8.02 Software (GraphPad Software, inc., San Diego, California, USA). All group tests data were subjected to the normality test (Kolmogorov-Smirnov test) and the homogeneity of variance test (Levene test) before parameter testing. A one-way anova was applied with the check level set at p < 0.05.
5. Results of the experiment
The total course in the open field experiment reflects the motor ability of the animal, the statistical result is shown in fig. 6, the result shows that the two have no significant difference, but the total course collected by the anxiety triple composite behavior detection device is more than that of the traditional open field experiment device, and the motor ability of the animal can be more accurately reflected.
The distance in the central area of the open field experiment reflects the depression/anxiety-like behaviors of the animals, the shorter the stay time is, the more the animals are depressed/anxious (prefer to move in the surrounding area), the statistical result is shown in fig. 7, the result shows that the two are significantly different, the distance data in the central area of the open field acquired by the anxiety triple composite behavior detection device is superior to that of the traditional open field experiment device, and the results of C57 mice and KM mice are consistent.
The number of times of entering the open field experiment central area reflects depression/anxiety-like behaviors of the animals, the smaller the number of times of entering the open field experiment central area, the more depressed/anxious animals are shown (the animals prefer to move in the peripheral area), the statistical result is shown in fig. 8, the results show that the statistics result shows that the statistical result shows that the number of times of entering the open field central area is superior to that of the traditional open field experiment device, and the C57 mice and the KM mice have the same result.
The percentage of time (OT%) for the elevated plus maze experiment to enter the open arm and the number of times (OE%) for the elevated plus maze experiment to enter the open arm reflect the depression/anxiety-like behaviors of the animal, the smaller the percentage is, the more depression/anxiety the animal is (preferring to move in the closed area), the statistical results are shown in figures 9 and 10, the results show that the two are significantly different, and the elevated plus maze experiment data collected by the anxiety triple composite behavior detection device is superior to the traditional elevated plus maze device, and the results of the C57 mouse and the KM mouse are consistent.
The distance and the entering time of the bright area of the light and dark box experiment reflect the depression/anxiety-like behaviors of the animal, the smaller the distance and the time, the more the depression/anxiety of the animal is (the animal prefers to move in the dark area), the statistical results are shown in figures 11 and 12, the results show that the distance and the entering time of the bright area of the light and dark box experiment data acquired by the anxiety triple composite behavior detection device are superior to those of the traditional light and dark box device, and the results of a C57 mouse and a KM mouse are consistent.
The behavior of the C57 and KM mice during the 15 minute trial in the anxiety triple combination behavioral testing apparatus is detailed in figures 6-12: the behavior of the total road course of the open field is shown in fig. 6, the behavior of the central area of the open field is shown in fig. 7, the behavior of the number of times of entering the central area of the open field is shown in fig. 8, the number of times and the percentage of time of entering the open arms of the elevated plus maze are shown in fig. 9 and 10, the entering path of the bright area of the light and dark box is shown in fig. 11, and the staying time of the bright area of the light and dark box is shown in fig. 12.
The above experimental data confirm that: the effect of the experimental animal anxiety triple composite behavior detection device is superior to that of the traditional behavioral test, the triple test provides wider measures by providing synchronous measurement from three different devices, and experimental data are more real and objective, so that fewer special results are generated, and the experimental animal anxiety triple composite behavior detection device is worthy of popularization and application.
Synchronous measurement here means that the mouse can freely pass through three devices, where behavioural data are collected simultaneously.
Claims (7)
1. A experimental animal anxiety triple composite behavior detection device is characterized in that: including base, spacious field case, overhead maze, light and shade case and support, base upper surface fixed connection have the support, the top of support from left to right in proper order spacious field case, overhead maze and light and shade case, spacious field case, overhead maze and light and shade case interconnect in proper order to communicate through first passageway, second passageway respectively, the support hide the below at spacious field case, overhead maze, light and shade case respectively.
2. The experimental animal anxiety triad compound behavior detection device as claimed in claim 1, wherein: the open-field box is of a box body structure with an open top end, and the upper surface of the box bottom is divided into 1 central area and 8 peripheral areas by transverse and vertical crossed lines; the light and dark box sequentially comprises a dark box and a light box which are mutually attached and fixedly connected from left to right, the dark box and the light box are both of a closed structure, the dark box is communicated with the light box through a third channel, the third channel penetrates through the bottom of the side wall of the dark box connected with the light box, and the bottom of the side wall of the dark box, which faces one side of the elevated labyrinth, is provided with a second channel and is communicated with the elevated labyrinth through the second channel; the side wall bottom of the open field box towards one side of the elevated labyrinth is provided with a first channel which is communicated with the elevated labyrinth through the first channel.
3. The experimental animal anxiety triad compound behavior detection device as claimed in claim 2, wherein: the inner wall of the dark box is coated with black and is internally provided with a red illuminating lamp, and the inner wall of the bright box is coated with white and is internally provided with a white illuminating lamp.
4. The triple-combination anxiety behavior test device for laboratory animals according to claim 3, wherein: the elevated labyrinth is a cross elevated labyrinth, comprises a first cantilever arranged along the left and right directions and a second cantilever arranged along the front and back directions and forming a cross structure with the first cantilever, the cross section of the first cantilever is of a groove structure, the cross section of the second cantilever is of a square structure, and a platform is arranged at the central position of the cross connection of the first cantilever and the second cantilever.
5. The triple-combination anxiety behavior test device for laboratory animals according to claim 3, wherein: the elevated labyrinth is a horizontally arranged circular labyrinth, the left end and the right end of the circular labyrinth are respectively provided with a first groove section, the opposite ends of the 2 first groove sections are respectively provided with a first platform section, and the 2 first groove sections are respectively communicated with an open field box and a light and dark box.
6. The triple-combination anxiety behavior test device for laboratory animals according to claim 3, wherein: the elevated labyrinth is a straight-line labyrinth, which comprises second groove sections positioned at two ends and a second platform section connected between the 2 second groove sections, wherein the 2 second groove sections are respectively communicated with the open field box and the light and dark box.
7. The triple-combination experimental animal anxiety behavior detection device as claimed in any one of claims 1 to 6, wherein: the base is arranged between the base and the bracket; the bracket is respectively arranged between the open field box, the light and dark box and the elevated labyrinth; the elevated labyrinth, the light and dark box and the open field box are respectively detachably and fixedly connected through bolts.
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