CN114908004A - Bifidobacterium infantis capable of preventing and/or treating atopic dermatitis and application thereof - Google Patents
Bifidobacterium infantis capable of preventing and/or treating atopic dermatitis and application thereof Download PDFInfo
- Publication number
- CN114908004A CN114908004A CN202210433128.1A CN202210433128A CN114908004A CN 114908004 A CN114908004 A CN 114908004A CN 202210433128 A CN202210433128 A CN 202210433128A CN 114908004 A CN114908004 A CN 114908004A
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- Prior art keywords
- bifidobacterium infantis
- atopic dermatitis
- rego
- mice
- food
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Abstract
The invention discloses bifidobacterium infantis capable of preventing and/or treating atopic dermatitis and application thereof, wherein the bifidobacterium infantis is identified as bifidobacterium infantis (Bifidobacterium infantis)Bifidobacterium infantis) The strain is named as REGO and is preserved in China center for type culture Collection with the preservation date of 2021, 03 and 24 days and the preservation number of CCTCC No. M2021268. The bifidobacterium infantis provided by the invention has good growth characteristics, can improve the swelling degree of atopic dermatitis, reduce the total IgE level in serum and the degranulation phenomenon of mast cells, recover the immune balance between Th1/Th2 in local tissues, remarkably reduce the release of histamine in the local tissues, and relieve the skin pruritus symptom, thereby relieving the inflammation of the atopic dermatitis; the product is prepared into a corresponding product for relieving atopic dermatitis, and can remarkably relieve symptoms and inhibit inflammation; therefore, the bifidobacterium infantis can be used for preparing medicines, functional foods and food/medicine additive components capable of preventing and/or treating atopic dermatitis, and has high application value.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium infantis capable of preventing and/or treating atopic dermatitis and application thereof.
Background
Atopic Dermatitis (AD) is a chronic, recurrent, inflammatory skin disease that is often accompanied by severe itching and other allergic diseases. Meanwhile, AD is a common non-infectious skin disease affecting up to 20% of children and 2-8% of adults in most countries worldwide, and its clinical manifestations are diversified and can be divided into infancy, childhood, and adolescence according to its occurrence, development and distribution characteristics
The three stages of juvenile and adult, wherein most of AD patients occur in infancy, and serious patients can even continue to adult, which is one of worldwide public health problems due to the repeated serious influence on the life quality of the patients, and therefore needs to be paid more attention.
The manifestations of AD, similar to asthma and allergic rhinitis, are mainly associated with local infiltration of T lymphocytes secreting interleukin-4 (IL-4), interleukin-13 (IL-13), etc., whereas IL-4, IL-13, associated with the development of a phenotype that regulates helper T cell 2(Th2), results in immunoglobulin (Ig) overproduction and eosinophilia; also, elevated levels of total IgE and allergen-specific IgE are a characteristic feature of AD, which is shared by at least 50% of AD patients, and this IgE is primarily targeted to environmental allergens. Therefore, there are several abnormal immune response steps in the development and progression of AD, such as: presentation of allergens by langerhans cells and skin dendritic cells, abnormal immune response mainly including Th2, regulatory T cell dysfunction, excessive IgE production, increased eosinophils, and the like.
At present, no therapeutic drug with definite curative effect for AD exists for constipation, and the adopted solution mainly relieves the symptoms of AD by external drugs such as glucocorticoid drugs, calcineurin inhibitors, external antimicrobial preparations, antihistamines, anti-inflammatory mediator drugs, immunosuppressants and the like. However, the above drugs have great adverse reactions, and most patients have concerns about hormone drugs, which not only bring great difficulty to the treatment of AD, but also bring great trouble to the lives of patients, and in addition, the drugs have poor tolerance for individual patients.
Therefore, there is still a need for a drug or a treatment that does not cause side effects to patients, can be used to combat the onset of allergy and the development of skin inflammation, relieve the symptoms of skin itch, eczema and the like in patients, and can be applied to various types of patients with good tolerance to the patients. Probiotics are active microorganisms which are beneficial to a host and change the composition of flora at a certain part of the host by colonizing in a human body. Compared with common medicines, the probiotics has the advantages of high safety, no side effect, low cost and the like. Therefore, a method of alleviating AD by using probiotics would be an important means that can be preferentially taken and has low side effects. Research on the effect of bifidobacterium infantis on AD alleviation will have great influence on food science, microbiology, preventive medicine and other aspects, so that screening bifidobacterium infantis with an effect of alleviating AD is necessary.
Disclosure of Invention
The purpose of the invention is as follows: in view of the problems of the prior art, the invention aims to provide bifidobacterium infantis capable of preventing and/or treating atopic dermatitis and application thereof.
The technical scheme is as follows: in order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
bifidobacterium infantis identified as Bifidobacterium infantis (B.infantis)Bifidobacteriuminfantis) The strain is named as REGO and is preserved in China center for type culture Collection with the preservation date of 2021, 03 and 24 days, the preservation number is CCTCC No. M2021268, and the preservation address is Wuhan university in Wuhan Han China.
Bifidobacterium infantis: (Bifidobacterium infantis) The strain is derived from fresh excrement sample of healthy female infant, the 16S rDNA sequence of the strain is shown as SEQ ID NO.1 through sequencing analysis, the sequence obtained through sequencing is compared with the nucleic acid sequence in NCB, and the result shows that the strain is bifidobacterium infantis and is named as bifidobacterium infantis REGO (R) (C)Bifidobacterium infantisREGO)。
Bifidobacterium infantis: (Bifidobacterium infantis) The colonies on MRS medium are convex to pad, complete in edge, soft, moist, white and shiny, and have the following characteristics: .
(1) Significantly improve the ear swelling degree of atopic dermatitis mice;
(2) the total IgE level in the serum of the atopic dermatitis mouse is obviously reduced by regulating the immune system of the mouse in vivo;
(3) the method is favorable for reducing the mastocyte degranulation phenomenon in a mouse body so as to obviously improve the dermatitis inflammation of the atopic dermatitis mouse;
(4) significantly reducing the level of cytokines (IL-4 and IL-13) related to Th2 cells in local tissues of mice so as to restore the immune balance between Th1/Th2 in the local tissues of atopic dermatitis mice;
(5) significantly reduces the release of histamine in local tissues of the mice, relieves the skin itch symptom of the atopic dermatitis mice, and relieves the dermatitis inflammation of the atopic dermatitis mice.
The invention also provides application of the bifidobacterium infantis in preparing medicines or foods for preventing and/or treating atopic dermatitis. Or the application of the bifidobacterium infantis in preparing medicines or foods for relieving atopic dermatitis.
Preferably, in the medicine or food, the viable count of the bifidobacterium infantis is not less than 1 x 10 6 CFU/g or 1X 10 6 CFU/mL。
Preferably, the dosage form of the medicine is powder, granules, capsules, tablets, pills or oral liquid; the food is a health food; or the food comprises dairy products, bean products, meat products or fruit and vegetable products; or the food is a beverage or snack.
The invention also provides a composition containing the bifidobacterium infantis.
Preferably, the composition comprises the bifidobacterium infantis, a pharmaceutical carrier and/or a pharmaceutical excipient.
Further preferably, the drug carrier comprises microcapsules, microspheres, nanoparticles and/or liposomes; the pharmaceutic adjuvant comprises an excipient and/or an additive.
More preferably, the excipient comprises a binder, filler, disintegrant and/or lubricant; the additive comprises a solubilizer, a cosolvent and/or a preservative.
Preferably, the composition comprises the bifidobacterium infantis and edible auxiliary materials.
As an embodiment, the composition comprises the use of a composition comprising said Bifidobacterium infantis (b: (a))Bifidobacterium infantis) The dairy product, the bean product or the fruit and vegetable product are produced by the leaven; or comprises the Bifidobacterium infantis (Bifidobacterium infantis) The solid beverage of (1).
Preferably, the preparation method of the leaven is that bifidobacterium infantis (b)Bifidobacterium infantis) Inoculating the strain into a culture medium according to the inoculum size accounting for 2-4% of the total mass of the culture medium, and culturing at 37 +/-2 ℃ for 16 +/-2 h to obtain a culture solution; centrifuging the culture solution to obtain thalli; cleaning the bacteria with phosphate buffer solution with the pH value of 7.2-7.4, and then resuspending the bacteria with a freeze-drying protective agent to obtain a resuspension solution; and (4) freeze-drying the heavy suspension by adopting a vacuum freezing method to obtain the leavening agent.
Further preferred is:
the mass ratio of the freeze-drying protective agent to the thallus is 2: 1.
The culture medium comprises 87.5 percent of water, 12 percent of skim milk, 0.6 percent of glucose, 1.4 percent of tryptone and 0.4 percent of yeast extract solution by mass of the total mass of the culture medium.
The pH of the medium was 6.8.
The protective agent comprises 100g/L of skimmed milk powder, 150g/L of trehalose and 10g/L of L-sodium glutamate.
As another embodiment, the composition further comprises a bacterial agent obtained by drying a bacterial solution containing the bifidobacterium infantis. Further preferably, the drying adopts vacuum freeze drying or other bacteria liquid drying processes.
The invention finally provides the application of the composition in preparing a medicament or food for preventing and/or treating atopic dermatitis.
Has the advantages that: the bifidobacterium infantis provided by the invention has good growth characteristics, can improve the swelling degree of atopic dermatitis, reduce the total IgE level in serum and the degranulation phenomenon of mast cells, recover the immune balance between Th1/Th2 in local tissues, remarkably reduce the release of histamine in the local tissues, and relieve the skin pruritus symptom, thereby relieving the inflammation of the atopic dermatitis; the product is prepared into a corresponding product for relieving atopic dermatitis, and can remarkably relieve symptoms and inhibit inflammation; therefore, the bifidobacterium infantis can be used for preparing medicines, functional foods and food/medicine additive components capable of preventing and/or treating atopic dermatitis, and has high application value.
Drawings
FIG. 1: comparison of the effect of different groups on reducing the thickness of the ears in atopic dermatitis mice.
FIG. 2: comparison of the effects of different groups on reducing the serum total IgE levels of atopic dermatitis mice.
FIG. 3: comparison of the effects of different groups on the reduction of IL-4 levels in the skin tissues of atopic dermatitis mice.
FIG. 4: comparison of the effects of different groups on the reduction of IL-13 levels in the skin tissues of atopic dermatitis mice.
FIG. 5: comparison of the effects of different groups on reducing histamine levels in skin tissues of atopic dermatitis mice.
Detailed Description
The invention is further illustrated by the following examples. These examples are purely illustrative and they are intended to describe the invention in detail only and should not be interpreted as limiting the invention. The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.
The media referred to in the examples below are as follows.
MRS solid medium (g/L): 10g/L of peptone, 10g/L of beef extract, 20g/L of glucose, 2g/L of sodium acetate, 5g/L of yeast powder and 2g/L, K of diammonium hydrogen citrate 2 PO 4 ·3H 2 O 2.6g/L、MgSO 4 ·7H 2 O 0.1g/L、MnSO 4 0.05g/L, Tween 801 mL/L, agar 20g/L and cysteine hydrochloride 0.5 g/L.
MRS liquid medium (g/L): 10g/L of peptone, 10g/L of beef extract, 20g/L of glucose, 2g/L of sodium acetate, 5g/L of yeast powder and 2g/L, K of diammonium hydrogen citrate 2 PO 4 ·3H 2 O 2.6g/L、MgSO 4 ·7H 2 O.1g/L, MnSO40.05g/L, Tween 801 mL/L, cysteine hydrochloride 0.5 g/L.
Example 1: and (3) screening the bifidobacterium infantis and identifying strains.
1. Screening
Taking excrement of healthy female infants from Sagao Rugao city in Jiangsu province as a sample, pretreating the sample, storing the pretreated sample in about 20% of glycerol in a refrigerator at the temperature of-80 ℃, taking out the sample, thawing the sample, uniformly mixing the sample, sucking 0.5mL of the sample, adding the sample into 4.5mL of the sample, performing gradient dilution by using 0.9% of normal saline containing 0.05% of cysteine, selecting a proper gradient diluent to be coated on an MRS solid culture medium added with 0.05% of cysteine, culturing the sample at the temperature of 37 ℃ for 48 hours, selecting a typical colony, streaking and purifying the typical colony on the MRS solid culture medium, selecting a single colony, transferring the single colony to an MRS liquid culture medium (containing 0.05% of cysteine) for enrichment, and preserving the single colony by using 30% of glycerol to obtain a strain REGO.
2. Identification
Extracting genome of REGO, amplifying and sequencing 16S rDNA of REGO, wherein the nucleotide sequences amplified by 16S rDNA of REGO are respectively shown as SEQ ID NO.1, and the sequence is subjected to nucleic acid sequence comparison in NCBI, and the result shows that the strain is bifidobacterium infantis and named as bifidobacterium infantis REGO (REGO) ((R))Bifidobacterium infantisREGO)。
The obtained Bifidobacterium infantis is classified and named as Bifidobacterium infantis REGO (B)Bifidobacteri- um infantisREGO) The strain has been preserved in China center for type culture Collection (preservation center of Wuhan university) at 31.3.31.2021, with the preservation number of CCTCC No. M2021268 and the preservation address of No. 299 in the Wuhan City, Hubei province.
Example 2: culture of Bifidobacterium infantis
Bifidobacterium infantis (Bifidobacterium infantis) The bacterial colony is observed after the REGO is inoculated into an MRS solid culture medium (containing 0.05% cysteine) and cultured for 48 hours at 37 ℃, and the bacterial colony is found to be convex to pad-shaped, complete in edge, soft, moist, white and glossy.
Bifidobacterium infantis (Bifidobacterium infantis) Inoculating REGO into MRS liquid culture medium (containing 0.05% cysteine), anaerobically culturing at 37 deg.C for 24 hr, transferring into fresh MRS liquid culture medium (containing 0.05% cysteine), culturing under the same conditions for 24 hr, centrifuging thallus at 6000g for 15min, washing thallus with 0.9% physiological saline at 6000g, centrifuging again at 6000g for 10min to obtain thallus, and resuspending with 30% sucrose solutionAnd freezing at-80 deg.c for use. The experimental results are as follows: the initial viable count is 1 × 10 11 CFU/mL, viable count 1X 10 after 1 week 11 CFU/mL, the order of magnitude does not change, show that the bacterial liquid does not influence the experiment after being frozen, and can be used for animal experiments.
Example 3: effect of Bifidobacterium infantis on ear thickness in atopic dermatitis mice
30 healthy female C57BL/6 mice weighing 18-20g were randomly divided into 5 groups of 10 mice each, and 3 groups were: a blank group (control group), a model group administered with 2, 4-dinitrofluorobenzene (2, 4-dinitrofluorobenzene, DNFB), and a REGO group administered with Bifidobacterium infantis REGO, wherein the REGO group is a treatment group. The experiment was carried out for four weeks: the first week is the adaptation period of the mice; beginning intragastric administration in the second week until the experiment is finished, and respectively performing intragastric administration on the Bifidobacterium infantis REGO bacterial liquid in the treatment groups by 0.2mL (the total amount of viable bacteria in single intragastric administration is 1 × 10) 9 Performing gavage at the dosage of CFU)/one time, wherein the blank group and the model group are not subjected to bacterial liquid intervention, and only the same amount of physiological saline is used as a control; the third week to the fourth week are modeling periods, the model group and the right ear of the mouse in the treatment group are smeared with 50 mu L of DNFB solution with the concentration of 0.5% for skin lesion sensitization and excitation on the 1 st day of modeling, the model group and the right ear of the mouse in the treatment group are smeared with 20 mu L of DNFB solution with the concentration of 0.2% on the 5 th, 8 th, 11 th and 14 th days, and the right ear of the mouse in the blank group is smeared with the same amount of acetone/olive oil matrix solution as a control; all groups were free water and food intake.
The ear thickness of 5 groups of mice was measured by a digital display micrometer screw before the start of molding (i.e., day 0), and after the end of molding (i.e., day 15), blood was taken and the mice were sacrificed, and then the ear thickness of the mice was immediately measured, and the measurement results are shown in fig. 1.
As shown in FIG. 1, Bifidobacterium infantis REGO can significantly reduce the thickness of the ears of mice with atopic dermatitis, and relieve the swelling degree of the ears of the mice. The above experimental results show that bifidobacterium infantis REGO has the effect of relieving the thickness and swelling degree of the ears of atopic dermatitis mice.
Example 4: effect of Bifidobacterium infantis on eosinophil infiltration in atopic dermatitis mice
30 healthy female C57BL/6 mice weighing 18-20g were randomly divided into 5 groups of 10 mice each, and 3 groups were: a blank group (control group), a model group administered with 2, 4-dinitrofluorobenzene (2, 4-dinitrofluorobenzene, DNFB), and a REGO group administered with Bifidobacterium infantis REGO, wherein the REGO group is a treatment group.
The experiment was carried out for four weeks: the first week is the adaptation period of the mice; beginning intragastric administration in the second week until the experiment is finished, and respectively performing intragastric administration on the Bifidobacterium infantis REGO bacterial liquid in the treatment groups by 0.2mL (the total amount of viable bacteria in single intragastric administration is 1 × 10) 9 Performing gavage at the dosage of CFU)/one time, wherein the blank group and the model group are not subjected to bacterial liquid intervention, and only the same amount of physiological saline is used as a control; the third week to the fourth week are modeling periods, the skin on the back of 5 groups of mice is depilated on the 1 st day of modeling, the area is about 2.5cm multiplied by 2.5cm, 50 mu L of DNFB solution with the concentration of 0.5% is used for coating the hair removal area on the back of the model group and the hair removal area on the back of the mice of the treatment group for skin damage sensitization and excitation, 20 mu L of DNFB solution with the concentration of 0.2% is used for coating the hair removal area on the back of the model group and the hair removal area on the back of the mice of the treatment group on the 5 th day, the 8 th day, the 11 th day and the 14 th day, and the hair removal area on the back of the mice of the blank group is only coated with the same amount of acetone/olive oil matrix solution as a control; all groups were free water and food intake.
After the molding is finished (i.e. day 15), blood is taken and the mice are sacrificed, and the serum total IgE level of the serum is measured by an enzyme-linked immunosorbent assay kit (the result is shown in figure 2).
As can be seen from fig. 2, bifidobacterium infantis REGO significantly reduced the serum total IgE level of mice compared to the model group, and was beneficial to suppress Th2 type immune response, thereby helping to alleviate the pathological inflammation of atopic dermatitis. The experiments show that the bifidobacterium infantis REGO can obviously reduce the total IgE level in the serum of mice and is beneficial to relieving the pathological inflammation of atopic dermatitis.
Example 5: effect of Bifidobacterium infantis on IL-4, IL-13 levels in skin tissues of atopic dermatitis mice
30 healthy female C57BL/6 mice weighing 18-20g were randomly divided into 5 groups of 10 mice each, and 3 groups were: a blank group (control group), a model group administered with 2, 4-dinitrofluorobenzene (2, 4-dinitrofluorobenzene, DNFB), and a REGO group administered with Bifidobacterium infantis REGO, wherein the REGO group is a treatment group.
The experiment was carried out for four weeks: the first week is the adaptation period of the mice; beginning intragastric administration in the second week until the experiment is finished, and respectively performing intragastric administration on the Bifidobacterium infantis REGO bacterial liquid in the treatment groups by 0.2mL (the total amount of viable bacteria in single intragastric administration is 1 × 10) 9 Performing gavage at the dosage of CFU)/one time, wherein the blank group and the model group are not subjected to bacterial liquid intervention, and only the same amount of physiological saline is used as a control; the third week to the fourth week are modeling periods, the skin on the back of 5 groups of mice is depilated on the 1 st day of modeling, the area is about 2.5cm multiplied by 2.5cm, 50 mu L of DNFB solution with the concentration of 0.5% is used for coating the hair removal area on the back of the model group and the hair removal area on the back of the mice of the treatment group for skin damage sensitization and excitation, 20 mu L of DNFB solution with the concentration of 0.2% is used for coating the hair removal area on the back of the model group and the hair removal area on the back of the mice of the treatment group on the 5 th day, the 8 th day, the 11 th day and the 14 th day, and the hair removal area on the back of the mice of the blank group is only coated with the same amount of acetone/olive oil matrix solution as a control; all groups were free water and food intake.
After the molding is finished (namely, day 15), blood is taken and the mice are sacrificed, then skin tissues of depilated areas on the backs of the mice are taken, and the levels of Th2 type immunoreaction cytokines IL-4 and IL-13 are measured by an enzyme-linked immunosorbent assay kit (the results are shown in figures 3-4).
As can be seen from fig. 3-4, bifidobacterium infantis REGO significantly reduced the levels of IL-4 and IL-13 in mouse skin tissues and significantly suppressed Th2 type immune response, compared to the model group, thereby contributing to the alleviation of pathological inflammatory conditions caused by Th2 type abnormal immune response. The experiments show that the bifidobacterium infantis REGO can obviously reduce the levels of cytokines IL-4 and IL-13 related to Th2 type immune response, and is helpful for relieving pathological inflammation states caused by Th2 type abnormal immune response.
Example 6: effect of Bifidobacterium infantis on Histamine levels in skin tissues of atopic dermatitis mice
30 healthy female C57BL/6 mice weighing 18-20g were randomly divided into 5 groups of 10 mice each, and 3 groups were: a blank group (control group), a model group administered with 2, 4-dinitrofluorobenzene (2, 4-dinitrofluorobenzene, DNFB), and a REGO group administered with Bifidobacterium infantis REGO, wherein the REGO group is a treatment group.
The experiment was carried out for four weeks: the first week is the adaptation period of the mice; beginning intragastric administration in the second week until the experiment is finished, and respectively intragastric administration of Bifidobacterium infantis REGO bacterial solution in the treatment groups by 0.2mL (the total amount of viable bacteria in single intragastric administration is 1 × 10) 9 Performing gavage at the dosage of CFU)/one time, wherein the blank group and the model group are not subjected to bacterial liquid intervention, and only the same amount of physiological saline is used as a control; the third week to the fourth week is a molding period, the skin on the back of 5 groups of mice is depilated on the 1 st day of molding, the area is about 2.5cm multiplied by 2.5cm, 50 microliter of DNFB solution with the concentration of 0.5% is smeared on the depilated areas on the back of the mice of the treatment group for skin damage sensitization and excitation, 20 microliter of DNFB solution with the concentration of 0.2% is smeared on the depilated areas on the back of the mice of the treatment group on the 5 th day, the 8 th day, the 11 th day and the 14 th day, and the depilated areas on the back of the mice of the blank group are smeared with the same amount of acetone/olive oil matrix solution as a control; all groups were free water and food intake.
After the molding is finished (namely day 15), blood is taken and the mice are sacrificed, then skin tissues of the depilated area on the back of the mice are taken, and inflammatory factors related to the degranulation condition of the mast cells are measured by an enzyme-linked immunosorbent assay kit: histamine (results are shown in figure 5).
As can be seen from fig. 5, bifidobacterium infantis REGO significantly reduced the level of histamine in skin tissues of mice compared to the model group, so that it can be concluded that bifidobacterium infantis REGO significantly reduced the degranulation of mast cells, prevented the release of histamine in skin damaged tissues, and alleviated the skin itching condition of mice. The above experiments show that bifidobacterium infantis REGO can significantly reduce the level of histamine in skin tissues and inhibit the degranulation condition of mast cells in the skin tissues, thereby relieving the pruritus of the skin of mice and preventing the further development of atopic dermatitis.
Example 7: preparation method of solid beverage containing Bifidobacterium infantis REGO
Inoculating bifidobacterium infantis REGO into a culture medium according to the inoculation amount accounting for 3% of the total mass of the culture medium, and culturing at 37 ℃ for 18 hours to obtain a culture solution; centrifuging the culture solution to obtain thalli; will be culturedCentrifuging the solution to obtain thalli; cleaning the bacteria for 3 times by using a phosphate buffer solution with the pH value of 7.2-7.4, and then re-suspending the bacteria by using a trehalose freeze-drying protective agent with the trehalose concentration of 100g/L (the mass ratio of the freeze-drying protective agent to the bacteria is 2:1) to obtain a re-suspension; freeze-drying the heavy suspension by adopting a vacuum freezing method to obtain bifidobacterium infantis REGO bacterial powder; wherein the culture medium comprises 87.7 percent of water, 10 percent of enzyme hydrolysis skim milk, 0.5 percent of glucose, 1.5 percent of tryptone and 0.3 percent of yeast extract solution by mass of the total culture medium; the pH of the medium was 6.8; will contain 1X 10 10 And (3) mixing the CFU bifidobacterium infantis REGO powder with maltodextrin, wherein the total mass of the powder and the maltodextrin is 1g, so as to obtain the solid beverage rich in the bifidobacterium infantis REGO. Taking 10g of the solid beverage containing the bifidobacterium infantis REGO, re-dissolving the solid beverage with physiological saline, fixing the volume to 20mL, and feeding each mouse with 0.2mL of stomach every day for three weeks, so that the symptom of atopic dermatitis of the mouse can be effectively relieved, and the solid beverage has excellent effect on preventing and/or treating the atopic dermatitis.
Example 8: fermented food containing bifidobacterium infantis REGO prepared by using the same
Selecting fresh vegetables or fruits, cleaning, and squeezing to obtain juice, wherein the juice comprises one or more of Chinese cabbage, white radish, cucumber, beet and yellow peach products as raw materials. Sterilizing the juiced raw materials at high temperature instantly, sterilizing at 140 deg.C for 2 s, immediately cooling to 37 deg.C, inoculating Bifidobacterium infantis REGO or microbial agent leaven containing the strain to make the concentration of Bifidobacterium infantis REGO reach 10 8 More than CFU/mL, and refrigerating and storing at the temperature of 4 ℃ to obtain the fruit and vegetable beverage containing the live bifidobacterium infantis REGO bacteria. The method comprises the step of producing fermented food by using bifidobacterium infantis REGO as a fermenting microorganism, wherein the fermented food comprises solid food, liquid food and semi-solid food. The fermented food comprises dairy products, bean products and fruit and vegetable products, wherein the dairy products comprise fermented dairy products, cream, cheese, milk-containing beverages and milk powder; the bean product comprises soybean milk and soybean milk powder; the fruit and vegetable products comprise Chinese cabbage, white radish, cucumber, beet and yellow peach products. The prepared fermented food is effective in relievingThe symptoms of atopic dermatitis in mice, has an excellent effect in preventing and/or treating atopic dermatitis.
The applicant states that the present invention is illustrated in detail by the above examples, but the present invention is not limited to the above detailed methods, i.e. it is not meant that the present invention must rely on the above detailed methods for its implementation. It should be understood by those skilled in the art that any modifications of the present invention, equivalent substitutions of the raw materials of the product of the present invention, and the addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Sequence listing
<110> Zhenjiang beneficial bacteria engineering biology Co., Ltd
<120> Bifidobacterium infantis capable of preventing and/or treating atopic dermatitis and use thereof
<141> 2022-04-22
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gtccggtgtg aaagtccatc gcctaacggt ggatctgcgc cgggtacggg cgggctggag 660
tgcggtaggg gagactggaa ttcccggtgt aacggtggaa tgtgtagata tcgggaagaa 720
caccaatggc gaaggcaggt ctctgggccg tcactgacgc tgaggagcga aagcgtgggg 780
agcgaacagg attagatacc ctggtagtcc acgccgtaaa cggtggatgc tggatgtggg 840
gccctttcca cgggtcccgt gtcggagcca acgcgttaag catcccgcct ggggagtacg 900
gccgcaaggc taaaactcaa agaaattgac gggggcccgc acaagcggcg gagcatgcgg 960
gattaattcg atgcaacgcg aaggaccctt acctggggct tgacatgtgc cggatcgccg 1020
tggagacacg gtttcccttc ggggccggtt cacaggtggt tgcatggtcg gtcgtcagct 1080
cgtgttcgtg agatgtttgg gtaagttccc gcacgacgca ccctcgccgc atgtttggca 1140
acggttgatg ccggaaacct catgtgggac cgcggggtca cctcggaagg gagggtggga 1200
atgaccgtca cgattc 1216
Claims (9)
1. A Bifidobacterium infantis characterized in that it is identified as Bifidobacterium infantis: (A), (B), (C)Bifidobacterium infantis) The strain is named as REGO and is preserved in China center for type culture Collection with the preservation date of 2021, 03 and 24 days and the preservation number of CCTCC No. M2021268.
2. Use of Bifidobacterium infantis according to claim 1 for the preparation of a medicament or food for the prophylaxis and/or treatment of atopic dermatitis.
3. The use according to claim 2, wherein the medicament or food product has a viable count of Bifidobacterium infantis of not less than 1 x 10 6 CFU/g or 1X 10 6 CFU/mL。
4. The use according to claim 2, wherein the food product is a health food; or the food comprises dairy products, bean products, meat products or fruit and vegetable products.
5. A composition comprising bifidobacterium infantis as claimed in claim 1.
6. Composition according to claim 5, characterized in that it comprises Bifidobacterium infantis as claimed in claim 1, a pharmaceutical carrier and/or a pharmaceutical excipient.
7. The composition of claim 5, wherein the composition comprises Bifidobacterium infantis as in claim 1 and a dietary supplement.
8. The composition according to claim 5, wherein the composition comprises a bacterial preparation obtained by drying a bacterial solution containing Bifidobacterium infantis according to claim 1.
9. Use of the composition according to claim 5 for the preparation of a medicament or food for the prevention and/or treatment of atopic dermatitis.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115518080A (en) * | 2022-10-20 | 2022-12-27 | 微康益生菌(苏州)股份有限公司 | Application of bifidobacterium lactis BLA80 in improving and relieving anaphylactic reaction |
-
2022
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115518080A (en) * | 2022-10-20 | 2022-12-27 | 微康益生菌(苏州)股份有限公司 | Application of bifidobacterium lactis BLA80 in improving and relieving anaphylactic reaction |
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