CN114907977B

CN114907977B - Support frame and biological reaction device

Info

Publication number: CN114907977B
Application number: CN202210807610.7A
Authority: CN
Inventors: 于搏洋; 李飙; 许俊; 胡福林; 易帅; 丁晨; 唐辉; 徐江运; 徐龙渤; 徐茏林
Original assignee: Zhejiang JYSS Bio Engineering Co Ltd
Current assignee: Zhejiang JYSS Bio Engineering Co Ltd
Priority date: 2022-07-11
Filing date: 2022-07-11
Publication date: 2022-11-01
Anticipated expiration: 2042-07-11
Also published as: CN114907977A

Abstract

The invention relates to a support frame and a biological reaction device. The support frame is used for supporting the biological reaction bag in the culture tank, the support frame can be fixedly arranged on the inner wall of the culture tank or the outer wall of the biological reaction bag, and the support frame is arranged between the biological reaction bag and the culture tank; the support frame comprises a support part, a supporting part which is raised compared with the inner wall of the culture tank is arranged on the support part, and the supporting part is used for supporting the biological reaction bag relative to the culture tank; above-mentioned support frame, the supporting part that swells through the inner wall for the culture tank props up biological reaction bag for the culture tank to reduce or even eliminate biological reaction bag's fold, avoid the fold to influence the culture solution and wash away biological reaction bag inner wall on the one hand, increase the dissolved oxygen effect among the culture process, on the other hand avoids the fold because of cell culture solution washes away biological reaction bag for a long time, leads to fold department to take place plastic deformation and form the "white point" of leaking easily.

Description

Support frame and biological reaction device

Technical Field

The invention relates to the technical field related to a biological reaction device, in particular to a support frame and a biological reaction device.

Background

The bioreactor bag is a bag-packaging device prepared from a membrane material, is widely applied to laboratory research and commercial production operation, and can be particularly used for production operation of ready-to-use cell culture, purification, fluid treatment and the like.

The liquid-filled bioreactor bag is filled into a culture tank, and particularly, a large-capacity bioreactor bag inevitably produces wrinkles on a membrane material on the bottom surface of the bioreactor bag, which adversely affects a bioreactor process.

Disclosure of Invention

Accordingly, there is a need for a support frame and a bioreactor.

The invention provides a support frame, which is used for supporting a biological reaction bag in a culture tank, can be fixedly arranged on the inner wall of the culture tank or the outer wall of the biological reaction bag, and is arranged between the biological reaction bag and the culture tank; the support frame includes support piece, be equipped with on the support piece than the supporting part that the culture tank inner wall uplift, the supporting part be used for with the biological reaction bag for the culture tank props up.

Above-mentioned support frame, the supporting part that swells through the inner wall for the culture tank props up biological reaction bag for the culture tank to reduce or even eliminate biological reaction bag's fold, avoid the fold to influence the culture solution and wash away biological reaction bag inner wall on the one hand, increase the dissolved oxygen effect among the culture process, on the other hand avoids the fold because of cell culture solution washes away biological reaction bag for a long time, leads to fold department to take place plastic deformation and form the "white point" of leaking easily.

In one embodiment, the radial section of the support portion is arched, and the radial section of the support portion gradually decreases in the axial direction and tends to a point.

It can be understood that, the supporting part with the arched radial section can support the biological reaction bag and avoid new creases caused by the extrusion of the biological reaction bag by the edge of the outer surface of the supporting part when compared with the supporting part with the edge on the radial section. In addition, the radial section of the support part is gradually reduced along the axial direction of the support part and tends to be a little, and the change of the height of the support part in the axial direction is smoother in addition to the change of the radial section, so that the appearance of new creases can be further reduced.

In one embodiment, the support further comprises a baffle portion connected to one end of the support portion having a larger radial dimension, and/or the baffle portion is connected to one end of the support portion having a smaller radial dimension.

It will be appreciated that the flap portion can act as a mechanical reinforcement to prevent the support portion from being damaged by crimping in the radial direction or by deformation under pressure in the projection direction during use.

In one embodiment, the baffle part comprises a first baffle fixed at one end of the support part with a larger radial dimension, and the first baffle has a radian suitable for the cylindrical part of the culture tank.

It can be understood that, in the operation process of the bioreactor, under the action of centrifugal force, the bioreactor bag pushes the support part to abut against the inner wall of the culture tank, and the radian of the first baffle is matched with the cylindrical part of the culture tank, so that the support part can be tightly abutted against the inner wall of the cylindrical part, and the fixing firmness of the support frame is increased.

In one embodiment, the support further comprises a transition portion disposed between the first baffle and the support portion, and the transition portion is planar.

It will be appreciated that the transition portion prevents sharp edges from forming on the support member, which could damage the bioreactor bag.

In one embodiment, the baffle part comprises a first baffle and a second baffle, the first baffle is fixedly arranged at one end of the support part with a larger radial dimension, and the second baffle is fixedly arranged at one end of the support part with a smaller radial dimension; and the first baffle has a radian suitable for the cylindrical part of the culture tank.

It can be understood that the two ends of the supporting part are respectively provided with the first baffle and the second baffle, so that the mechanical strength of the supporting part can be greatly improved, and the deformation of the supporting part caused by the scouring of the culture solution in the operation process of the biological reaction device is avoided. In addition, at the biological reaction device operation in-process, under the effect of centrifugal force, the biological reaction bag can push support piece to butt in the culture tank inner wall, and the radian of first baffle is fit for with the cylinder portion of culture tank to guarantee that support piece can closely offset with the inner wall of cylinder portion, with the fixed firm degree that increases the support frame. The setting of second baffle can avoid the support frame sharp-pointed department to appear to prevent that the biological reaction bag is punctured to the support frame.

In one embodiment, the bottom of the first baffle plate and the bottom of the second baffle plate are in the same curved surface, and the curved surface is matched with the curved surface of the side, relatively close to the cylindrical part, of the circular truncated cone part of the culture tank.

It can be understood that the two curved surfaces are suitable for enabling the support frame to be more stably arranged on the culture tank, so that the stability of the support frame in the operation process of the biological reaction device is ensured.

In one embodiment, the support frame comprises a fixed structure connected to the support; the fixing structure comprises a male buckle and a female buckle, one of the male buckle and the female buckle is fixedly arranged on the supporting piece, and the other one of the male buckle and the female buckle is fixedly arranged on the biological reaction bag or the culture tank.

It can be understood that, through the fixed mode of son knot and box joint, easy operation, and can dismantle as required, increased the general usefulness of support frame.

In one embodiment, the support member is adhesively secured to the culture tank or the bioreactor bag; or the support piece is a metal piece and is welded and fixed on the culture tank; or the supporting piece is a plastic piece and is welded and fixed on the biological reaction bag.

The invention provides a biological reaction device, which comprises a culture tank, a biological reaction bag and the support frame, wherein the biological reaction bag is contained in the culture tank, the support frame is fixedly arranged on the inner wall of the culture tank or the outer wall of the biological reaction bag, and the support frame is used for supporting the biological reaction bag relative to the culture tank.

In one embodiment, the culture tank comprises a cylindrical part and a circular table part, the cylindrical part is connected to the large-diameter end of the circular table part and is positioned at the top of the circular table part, and the support frame is arranged on one side of the circular table part, which is relatively close to the cylindrical part.

It can be understood that the fold of the biological reaction bag mainly appears at the connecting position of the cylindrical section and the circular truncated cone section, and the connecting position of the cylindrical section and the circular truncated cone section of the biological reaction bag can be fully unfolded by the arrangement, so that the fold eliminating effect of the support frame is maximized.

In one embodiment, the support frame further includes a first baffle and a second baffle, the first baffle is fixedly disposed at one end of the support portion relatively close to the inner wall of the culture tank, and the second baffle is fixedly disposed at one end of the support portion relatively close to the inside of the culture tank.

It can be understood that the arrangement of the first baffle plate can increase the stability of the support frame when the support frame is abutted against the inner wall of the culture tank; the setting of second baffle can prevent that the biological reaction bag is punctured to the support frame.

Drawings

FIG. 1 is a schematic view showing the structure of a bioreactor bag according to an embodiment;

FIG. 2 is a schematic sectional view showing the structure of the culture tank and the support frame in one embodiment;

FIG. 3 is a partial schematic view showing the structure of a culture tank, a bioreactor bag, and a holder according to one embodiment;

FIG. 4 is a schematic view of a support frame according to an embodiment;

FIG. 5 is a schematic perspective view of the support of FIG. 4 at another angle;

FIG. 6 is a schematic perspective view of the support of FIG. 4 at another angle;

FIG. 7 is a schematic cross-sectional view of the support frame of FIG. 4;

FIG. 8 is an enlarged view of a portion of FIG. 2 at X;

FIG. 9 is a schematic cross-sectional view of the support frame of FIG. 4 along a longitudinal section of one of the bus bars;

FIG. 10 is a partial schematic structural view of a culture tank, a bioreactor bag and a support frame in another embodiment;

FIG. 11 is a partial schematic structural view of a culture tank, a bioreactor bag and a support frame in another embodiment;

fig. 12 is a partially enlarged view of a portion Y in fig. 11.

100. A support frame; 10. a support member; 11. a support portion; 12. a baffle portion; 121. a first baffle plate; 122. a second baffle; 13. a side edge; 14. a transition section; 20. a fixed structure; 21. an extension portion; 22. a sub-buckle; 23. a female buckle; 200. a culture tank; 210. a circular table portion; 220. a cylindrical portion; 300. a biological reaction bag; 310. welding edges; 320. a cylindrical section; 330. a circular table section; A. axial direction; B. radial direction; C. a projection direction; E. a bottom surface.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

It will be understood that when an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. When an element is referred to as being "secured to" another element, it can be directly secured to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "or/and" includes any and all combinations of one or more of the associated listed items.

The bioreactor device comprises a bioreactor and a bioreactor bag 300, wherein the bioreactor comprises a shaking table (not shown) and a culture tank 200, the bioreactor bag 300 is used for containing a culture solution, the bioreactor bag 300 is contained in the culture tank 200, and the shaking table can drive the culture tank 200 to move, so as to drive the culture solution to flow. However, when the bioreactor bag 300 filled with liquid is loaded into the culture tank 200, particularly, when the bioreactor bag 300 having a large capacity is loaded, wrinkles are inevitably generated on the membrane material of the bottom surface of the bioreactor bag 300, which may adversely affect the bioreactor process.

Based on this, please refer to fig. 1, fig. 2 and fig. 3, the present invention first provides a support frame 100, the support frame 100 is used for supporting a bioreactor bag 300 in a culture tank 200, the support frame 100 can be fixed on the inner wall of the culture tank 200 or the outer wall of the bioreactor bag 300, and the support frame 100 is disposed between the bioreactor bag 300 and the culture tank 200; the support frame 100 includes a support member 10, a support part 11 protruding from the inner wall of the culture tank 200 is provided on the support member 10, and the support part 11 is used for supporting the bioreactor bag 300 with respect to the culture tank 200.

Since the supporting portion 11 of the supporting member 10 is protruded with respect to the inner wall of the culture tank 200, when the bioreactor bag 300 is placed on the inner wall of the culture tank 200 and the supporting member 10, the supporting member 10 can press the bioreactor bag 300 corresponding to the supporting portion 11 toward the inside thereof through the supporting portion 11, i.e., the bioreactor bag 300 is supported with respect to the culture tank 200, thereby achieving the effect of reducing or even eliminating wrinkles of the bioreactor bag 300.

The folds of the biological reaction bag 300 are reduced, so that on one hand, the effect of the folds on scouring the inner wall of the biological reaction bag 300 by the cell culture solution can be avoided, and the dissolved oxygen effect in the culture process is increased; on the other hand, the phenomenon that plastic deformation occurs at the folds and 'white spots' are formed, which are easy to leak, due to the fact that the cell culture fluid washes the folds of the biological reaction bag 300 for a long time can be avoided.

In some embodiments, culture tank 200 is a culture tank in a torrent bioreactor.

In the operation process of the culture tank in the torrent bioreactor, the culture tank 200 is driven by the shaking table and does eccentric motion according to a certain eccentric distance and rotating speed, and the cell culture solution in the culture tank 200 generates turbulent flow similar to tidal effect.

The turbulent flow generated by the cell culture fluid continuously washes the inner wall of the biological reaction bag 300, so that the oxygen bubbles in the cell culture fluid are broken or divided into smaller bubbles by impact, thereby achieving the effect of increasing the dissolved oxygen amount and the dissolved oxygen efficiency in the cell culture fluid.

It can be understood that if there are wrinkles on the inner wall of the bioreactor bag 300, on the one hand, the effect of the cell culture fluid washing the inner wall of the bioreactor bag 300 is affected, and thus the dissolved oxygen effect is affected; on the other hand, the cell culture fluid washes the wrinkles of the bioreactor bag 300 for a long time, which causes stress accumulation at the wrinkles, eventually generates "white spots" of plastic deformation and fatigue marks accompanying the "shell type", and the wrinkles generating the "white spots" are prone to leak, thereby causing a large loss.

The traditional stirring paddle type bioreactor adopts the working principle that a stirring paddle positioned in the middle of the bioreactor rotates to drive cell culture solution to flow so as to increase the dissolved oxygen amount and the dissolved oxygen efficiency of the cell culture solution, and the dissolved oxygen of the bioreactor does not depend on the inner wall of a biological reaction bag, so that the influence of the inner wall wrinkles of the biological reaction bag on the work of the biological reaction bag can be almost ignored, and technicians in the field have no motivation to arrange the support frame on the basis of the stirring paddle type bioreactor.

Referring to fig. 3, 4, 5 and 6, in some embodiments, the radial section of the supporting portion 11 is arched, and the radial section of the supporting portion 11 gradually decreases along the axial direction and tends to a point.

For the convenience of the following description, the axial direction a will be defined in the direction in which the axis of the support portion 11 is located; defining any direction on a radial plane as a radial direction B; the radial section of the support portion 11 refers to a cross section of the support portion 11 formed on a plane perpendicular to the axial direction a.

The supporting part 11 with the arched radial section can support the biological reaction bag 300 and avoid new creases caused by the fact that the edges of the outer surface of the supporting part 11 extrude the biological reaction bag 300 when compared with the supporting part 11 with edges on the radial section; the arch shape may be a part of a circle or an ellipse, or may be another arc shape as long as the new fold is not generated by pressing the bioreactor bag 300, and the present application is not limited thereto. In addition, the radial section of the support portion 11 gradually decreases along the axial direction thereof and tends to trend to a point, and in addition to the change of the radial section, the change of the height of the support portion 11 in the axial direction is smoother in transition from large to small, so that the occurrence of new creases can be further reduced.

As shown in FIGS. 3, 4 and 7, for the convenience of the following description, the plane formed by the two sides of the support part 11 contacting the culture tank 200 is referred to as a bottom surface E, and a direction perpendicular to the bottom surface E is defined as a projection direction C.

In some embodiments, the projection of the supporting portion 11 along the projection direction C is substantially fan-shaped, the radial cross section of the supporting portion 11 can gradually converge along the axial direction a, and the end of the supporting portion 11 with smaller radial dimension is still arch-shaped. So set up, can enough prevent that the supporting part from too sharply stabbing bioreactor bag 300, can effectively avoid bioreactor bag 300 bottom surface and the emergence of the condition of the new crease of arch edge extrusion appearance again.

Referring to fig. 6 and 7, in some embodiments, the supporting member 10 further includes a baffle portion 12, the baffle portion 12 is connected to one end of the supporting portion 11 with a larger radial dimension, and/or the baffle portion 12 is connected to one end of the supporting portion 11 with a smaller radial dimension.

The baffle portion 12 may be directly connected to the support portion 11, or may be indirectly connected to the support portion 11 through another structure; the baffle portion 12 can serve as a mechanical reinforcement to prevent the support portion 11 from being damaged by curling in the radial direction B or being deformed by pressure in the projection direction C during use.

Of course, the baffle portion 12 may be provided at other positions in the axial direction A, for example, a reinforcing rib may be provided on the side of the support portion 11 relatively close to the inner wall of the culture tank to form the baffle portion 12, as long as the baffle portion 12 can perform a mechanical reinforcing function.

Referring to fig. 3 and 4, in some embodiments, the bottom of the baffle portion 12 and the two sides 13 of the supporting portion 11 along the axial direction a can abut against the inner wall of the culture tank 200, so as to increase the fitting stability of the support member 10 and the inner wall of the culture tank 200.

Referring to fig. 7 and 8, in some embodiments, the baffle portion 12 includes a first baffle 121 and a second baffle 122, the first baffle 121 is fixedly disposed at one end of the support portion 11 with a larger radial dimension, and the second baffle 122 is fixedly disposed at one end of the support portion 11 with a smaller radial dimension; the height h1 of the first baffle 121 is greater than the height h2 of the second baffle 122, and the first baffle 121 has a curvature corresponding to the cylindrical portion 220 of the culture tank 200.

Set up first baffle 121 and second baffle 122 respectively at the both ends of supporting part 11, can increase substantially support piece 10's mechanical strength, avoid supporting part 11 to produce deformation because the erodeing of culture solution in the bioreactor operation process. In addition, during the operation of the bioreactor, under the action of centrifugal force, the bioreactor bag 300 pushes the support member 10 to abut against the inner wall of the culture tank 200, and the radian of the first baffle 121 is matched with the cylindrical portion 220 of the culture tank 200, so as to ensure that the support member 10 can abut against the inner wall of the cylindrical portion 220, thereby increasing the fixing firmness of the support frame 100. The second baffle 122 is disposed to prevent the support frame 100 from being sharp, so as to prevent the support frame 100 from puncturing the bioreactor bag 300.

In addition, when the support frame 100 is fixed to the inner wall of the cylindrical portion 220 by bonding or welding the first baffle 121, the first baffle 121 is closely attached to the inner wall of the cylindrical portion 220, so that the bonding area of bonding or welding can be increased, and the support frame 100 can be fixed more firmly.

It is understood that, in other embodiments, only the first baffle 121 or only the second baffle 122 may be provided, which is not limited herein.

In some embodiments, the support 10 further comprises a transition 14. The transition portion 14 is disposed between the first baffle 121 and the support portion 11, and the transition portion 14 is a plane. So configured, the transition portion 14 can prevent a sharp edge from being formed on the support member 10, so as to prevent damage to the bioreactor bag 300.

Of course, in some other embodiments not shown, the transition portion 14 may not be provided, or the transition portion 14 with other shapes may be provided, which is not limited herein.

In some embodiments, the support 10 can be directly secured to the culture tank 200 or bioreactor bag 300.

For example, the support member 10 may be bonded to the culture tank 200 or the bioreactor bag 300 by the first barrier 121, may be bonded by the two side edges 13 of the support member 11 and the bottom edges of the first barrier 121 and the second barrier 122, or may be bonded by another additional structure, which is not limited herein.

Or the support member 10 is a metal member, and the support member 10 is welded and fixed to the culture tank 200; alternatively, the supporting member 10 is a plastic member, and the supporting member 10 is welded and fixed to the bioreactor bag 300. When the first barrier 121 is welded and fixed, the periphery thereof may be welded to the cylindrical portion 220 of the culture pot 200, the two sides 13 of the support part 11 and/or the bottom edge of the second barrier 122 may be welded to the circular truncated cone portion 210 of the culture pot 200, or the two sides and/or the bottom edge may be welded to another structure.

In some embodiments, the support member 10 may be connected to the culture tank 200 or the bioreactor bag 300 by other structures.

Referring to fig. 10, in some embodiments, the supporting frame 100 includes a fixing structure 20, the fixing structure 20 is connected to the supporting member 10; the fixing structure 20 includes an extension portion 21, the extension portion 21 is connected to the support member 10, the support member 10 is disposed on a side of the circular platform portion 210 of the culture tank 200 relatively close to the circular column portion 220, the extension portion 21 extends out of the culture tank 200, and the extended portion can be clamped or snap-fixed on the top of the peripheral wall of the culture tank 200.

In some embodiments, the extension portion of the extension portion 21 is U-shaped to achieve the connection between the support frame 100 and the culture tank 200 by being engaged with the top of the peripheral wall of the culture tank 200; of course, the extension portion 21 may have other shapes as long as it can be engaged with the culture tank 200.

In some embodiments, the extension portion of the extension portion 21 can be fixed to the culture tank 200 by adhesion, screw connection, or other conventional fixing means, so as to achieve the fixed connection between the support frame 100 and the culture tank 200.

In some embodiments, as shown in fig. 11 and 12, the support 100 includes a fixing structure 20, the fixing structure 20 being connected to the support 10; the fixing structure 20 includes a male buckle 22 and a female buckle 23, one of the male buckle 22 and the female buckle 23 is fixed on the support 10, and the other is fixed on the bioreactor bag 300 or the culture tank 200. Specifically, be equipped with the joint structure that can elastic deformation in the box 23, seted up the joint groove in the joint structure, be equipped with the arch on the sub-knot 22, this arch can stretch into the joint groove, and the joint structure passes through this arch of elasticity chucking. It is understood that the female button 23 may be disposed on the bioreactor bag 300, and the positions of the male button 22 and the female button 23 may be changed, which is not limited herein.

It can be understood that the support frame 100 is fixed by the clamping connection between the male buckle 22 and the female buckle 23, the operation is simple, and the support frame can be detached as required, so that the universality of the support frame 100 is improved.

In some embodiments, each support 10 may be fixed on the culture tank 200 or the bioreactor bag 300 by one or more snap fasteners, and a plurality of female fasteners 23 may also be fixed on the culture tank 200 or the bioreactor bag 300 circumferentially, so that the support 10 fixed with the male fasteners 22 can be snap-fixed to any female fastener 23 according to a folding position or an actual requirement, further increasing the versatility of the support frame 100.

Referring to fig. 1, 2 and 8, a second aspect of the present invention provides a bioreactor including a culture tank 200, a bioreactor bag 300 and the support frame 100, wherein the bioreactor bag 300 is contained in the culture tank 200, the support frame 100 is fixed on an inner wall of the culture tank 200 or an outer wall of the bioreactor bag 300, and the support frame 100 is used for supporting the bioreactor bag 300 relative to the culture tank 200.

The culture tank 200 is a culture tank in a torrent bioreactor, and the support frame 100 supports the biological reaction bag 300, so that wrinkles of the biological reaction bag 300 can be reduced or even eliminated, on one hand, the wrinkles can be prevented from influencing the effect of cell culture solution on flushing the inner wall of the biological reaction bag 300, and the dissolved oxygen effect in the culture process is increased; on the other hand, the problems of plastic deformation 'white spots' at the folds and finally leakage caused by the fact that the cell culture fluid washes the folds of the biological reaction bag 300 for a long time can be avoided.

As shown in FIGS. 2, 8 and 9, in some embodiments, the culture tank 200 includes a cylindrical portion 220 and a circular table portion 210, the cylindrical portion 220 is connected to a large-diameter end of the circular table portion 210 and is located at the top of the circular table portion 210, and the support frame 100 is disposed on a side of the circular table portion 210 relatively close to the cylindrical portion 220.

The shape of the bioreactor bag 300 is similar to that of the culture tank 200, and the bioreactor bag 300 also has a cylindrical section 320 and a truncated cone section 330, the cylindrical section 320 and the truncated cone section 330 form a welding edge 310 in the welding and forming process, the bioreactor bag 300 can be abutted against the inner wall of the culture tank 200 after being placed in the culture tank 200, and the wrinkles of the bioreactor bag 300 mainly appear at the connecting position of the cylindrical section 320 and the truncated cone section 330.

It can be understood that, by disposing the support frame 100 at a side of the circular truncated cone portion 210 relatively close to the cylindrical portion 220, the connection position of the cylindrical section 320 and the circular truncated cone section 330 of the bioreactor bag 300 can be sufficiently spread, thereby maximizing the wrinkle removing effect of the support frame 100.

It is understood that one or more support members 10 may be provided, and two or more support members 10 may be uniformly arranged around the joint of the cylindrical part 220 and the circular truncated part 210 in the culture tank 200, or may be arranged according to the actual folding condition.

In one embodiment, the first baffle 121 is fixed to the end of the support 11 relatively close to the inner wall of the culture tank 200, and the second baffle 122 is fixed to the end of the support 11 relatively close to the inner wall of the culture tank 200; the bottom of the first baffle 121 and the bottom of the second baffle 122 are formed in the same curved surface corresponding to the curved surface of the circular table portion 210 of the culture tank 200 on the side closer to the cylindrical portion 220, and the curvature of the first baffle 121 corresponds to the inner curvature of the cylindrical portion 220.

During the reaction of the culture tank 200, the bioreactor bag 300 and the support frame 100 have a tendency to move radially outward from the center of the culture tank 200 due to the centrifugal force. The bottom of the first baffle 121 and the bottom of the second baffle 122 are located on the same curved surface, the curved surface is matched with the curved surface on one side of the circular table part 210 of the culture tank 200, which is relatively close to the cylindrical part 220, and the radian of the first baffle 121 is matched with the internal radian of the cylindrical part 220, so that the first baffle 121 can be tightly attached to the inner wall of the cylindrical part 220, and the bottom of the support member 10 can be tightly abutted to the circular table part 210, therefore, in the reaction process of the culture tank 200, the bioreactor bag 300 extrudes the support frame 100 under the action of centrifugal force, and the support frame 100 is tightly abutted to the culture tank 200 under the action of centrifugal force, so that the support frame 100 and the culture tank 200 are relatively fixed, and the firmness and fixation degree of the support frame 100 is further increased.

The features of the above-described embodiments may be arbitrarily combined, and for the sake of brevity, all possible combinations of the features in the above-described embodiments are not described, but should be construed as being within the scope of the present disclosure as long as there is no contradiction between the combinations of the features.

It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that suitable changes and modifications of the above embodiments are within the scope of the claimed invention as long as they are within the spirit and scope of the present invention.

Claims

1. A support frame, characterized in that the support frame (100) is used for supporting a bioreactor bag (300) in a culture tank (200), the support frame (100) can be fixedly arranged on the inner wall of the culture tank (200) or the outer wall of the bioreactor bag (300), and the support frame (100) is arranged between the bioreactor bag (300) and the culture tank (200);

the support frame (100) comprises a support member (10), a support part (11) which is raised compared with the inner wall of the culture tank (200) is arranged on the support member (10), and the support part (11) is used for supporting the biological reaction bag (300) relative to the culture tank (200);

the support frame (100) further comprises a baffle plate part (12), the baffle plate part (12) comprises a first baffle plate (121), the radial section of the support part (11) is gradually reduced along the axial direction of the support part and tends to a little, and the first baffle plate (121) is fixedly arranged at one end of the support part (11) with a larger radial dimension.

2. Support according to claim 1, characterized in that the radial section of the support (11) is arched.

3. The support frame of claim 1, wherein the first baffle (121) has an arc shape conforming to the cylindrical portion (220) of the culture tank (200).

4. A support stand according to claim 3, wherein the support (10) further comprises a transition (14), the transition (14) being arranged between the first apron (121) and the support (11), and the transition (14) being planar.

5. A support bracket as claimed in claim 1, characterised in that the flap portion (12) further comprises a second flap (122), the second flap (122) being secured to the end of the support portion (11) having a relatively small radial dimension.

6. A support stand according to claim 5, wherein the bottom of the first apron (121) and the bottom of the second apron (122) are in the same curved surface which conforms to the curved surface of the circular table portion (210) of the culture tank (200) on the side which is relatively close to the cylindrical portion (220).

7. The support bracket of any one of claims 1 to 6, wherein the support bracket (100) comprises a fixed structure (20), the fixed structure (20) being connected to the support (10);

the fixing structure (20) comprises a sub-buckle (22) and a female buckle (23), one of the sub-buckle (22) and the female buckle (23) is fixedly arranged on the support member (10), and the other is fixedly arranged on the biological reaction bag (300) or the culture tank (200).

8. The support frame of any one of claims 1 to 6, wherein the support member (10) is adhesively secured to the culture tank (200) or the bioreactor bag (300); or the support (10) is made of metal, and the support (10) is welded and fixed on the culture tank (200); or, the supporting member (10) is a plastic member, and the supporting member (10) is welded and fixed to the biological reaction bag (300).

9. A bioreactor apparatus, comprising a culture tank (200), a bioreactor bag (300) and the support frame of any one of claims 1 to 8, wherein the bioreactor bag (300) is accommodated in the culture tank (200), the support frame (100) is fixedly arranged on the inner wall of the culture tank (200) or the outer wall of the bioreactor bag (300), and the support frame (100) is used for supporting the bioreactor bag (300) relative to the culture tank (200).

10. The bioreactor apparatus according to claim 9, wherein the culture tank (200) comprises a cylindrical portion (220) and a circular table portion (210), the cylindrical portion (220) is connected to a large diameter end of the circular table portion (210) and is located at a top of the circular table portion (210), and the support frame (100) is disposed at a side of the circular table portion (210) relatively close to the cylindrical portion (220).

11. The bioreactor apparatus according to claim 10, wherein the supporting frame (100) further comprises a second baffle plate (122), the first baffle plate (121) is fixed to an end of the supporting portion (11) relatively close to the inner wall of the culture tank (200), and the second baffle plate (122) is fixed to an end of the supporting portion (11) relatively close to the inner part of the culture tank (200).