CN114891618A

CN114891618A - Aseptic automatic sample injector of 96 orifice plates

Info

Publication number: CN114891618A
Application number: CN202210366276.6A
Authority: CN
Inventors: 邱元正; 袁文慧; 刘超; 李果; 刘勇; 卢善竑; 王芸芸
Original assignee: Xiangya Hospital of Central South University
Current assignee: Xiangya Hospital of Central South University
Priority date: 2022-04-08
Filing date: 2022-04-08
Publication date: 2022-08-12
Anticipated expiration: 2042-04-08
Also published as: CN114891618B

Abstract

The invention discloses a 96-pore plate sterile automatic sample injector which comprises a base, wherein one side of the base is connected with a sample plate to be injected in a sliding manner, a slot for the sample plate to be injected to slide is formed in the base, a liquid containing box for containing sample liquid is connected in the base in a sliding manner, a second sliding chute for the liquid containing box to slide is formed in the base, a plurality of groups of dripping holes are formed in the base, the dripping holes are communicated with the slot and the second sliding chute, a control assembly for controlling the sample liquid to flow out is arranged in the liquid containing box, a pressure rod is connected in the second sliding chute in a sliding manner, and a lifting mechanism for lifting the pressure rod is arranged in the base. Second dysmorphism piece extrusion extension rod for the depression bar extrudees the sliding plate, thereby makes to produce the gap between trapezoidal sprue and the liquid outlet nozzle, thereby makes to add sample liquid from the gap between trapezoidal sprue and the liquid outlet nozzle drip to treat every downthehole of application of sample board, and then realizes the application of sample in batches, avoids the application of sample error.

Description

Aseptic automatic sample injector of 96 orifice plates

Technical Field

The invention relates to the technical field of cell culture, in particular to a 96-pore plate sterile automatic sample injector.

Background

The cell culture series can promote the primary generation of cells: such as nerve cell passage cells, animal and human body cells such as tumor cells and the like are widely used in various colleges and universities, scientific research institutions and bioengineering companies at home and abroad, and a 96-hole cell culture plate made of imported optically transparent pure polystyrene may be selected in the cell culture process.

Among them 96-well plate is because the hole number is more, when the liquid in the hole plate is added with the sample, the traditional approach is that the operator adds to it one by one in proper order, and this is very time-consuming, and very easy causes the interpolation error, therefore needs a 96-well plate aseptic autosampler to solve above-mentioned problem urgently.

Disclosure of Invention

Objects of the invention

In view of this, the present invention provides an aseptic autosampler for 96-well plates, so as to realize batch loading of 96-well plates and reduce loading errors.

(II) technical scheme

In order to achieve the technical purpose, the invention provides a 96-pore plate sterile automatic sample injector which comprises the following components in parts by weight:

it includes the base, one side sliding connection of base remains to add the model, the gliding slot of confession application of sample board of treating is seted up to the inside of base, the inside sliding connection of base has the flourishing liquid box that is used for placing application of sample liquid, the gliding second spout of confession flourishing liquid box is seted up to the inside of base, the multiunit hole that drips has been seted up to the inside of base, the hole that drips is linked together with slot, second spout, the inside of flourishing liquid box is provided with and is used for controlling the control assembly who adds the outflow of sample liquid, the inside sliding connection of second spout has the depression bar, the inside of base is provided with carries out the elevating system who goes up and down to the depression bar.

It should be noted that, through having seted up the hole that drips, can be so that the drain nozzle inserts the hole that drips to let the drain nozzle be close to the top of waiting to add the model, thereby ensure accurate drippage, and reduce the long time that the application of sample liquid exposes outside, ensure the aseptic nature of application of sample ware.

Preferably, the control assembly comprises a sliding plate slidably connected with the inner walls of the left side and the right side of the liquid containing box, the left side and the right side of the sliding plate are fixedly connected with T-shaped sliding blocks, the inner walls of the left side and the right side of the liquid containing box are respectively provided with a T-shaped sliding chute for the T-shaped sliding blocks to slide, the inner walls of the left side and the right side of the liquid containing box are fixedly connected with a fixed plate, the bottom of the sliding plate is fixedly connected with a plurality of groups of regulating and controlling rods corresponding to the plurality of groups of dripping holes, the regulating and controlling rods penetrate through the fixed plate and are slidably connected with the fixed plate, a first elastic part is sleeved on the outer side of each regulating and controlling rod, and two ends of each first elastic part are respectively fixedly connected with the bottom side of the sliding plate and the top side of the fixed plate.

Preferably, the bottom fixedly connected with multiunit liquid outlet nozzle of flourishing liquid box, the liquid outlet that is linked together with the liquid outlet nozzle inside is seted up to the bottom of flourishing liquid box, the regulation and control pole runs through the liquid outlet and extends to the trapezoidal sprue of the inside one end fixedly connected with of liquid outlet nozzle, the outside of trapezoidal sprue supports tightly each other with the inner wall of liquid outlet nozzle.

Preferably, the first dysmorphism piece of first fixedly connected with in both sides about flourishing liquid box top, the gliding first spout of confession first dysmorphism piece is seted up to the inside of base, first spout is linked together with the second spout, and two sets of one side that first dysmorphism piece is close to each other has all seted up T type draw-in groove, the bottom side at both ends is all fixedly connected with T type fixture block about the depression bar, T type fixture block sliding fit in the inside of T type draw-in groove.

Preferably, all sliding connection has the extension rod in both ends about the depression bar, the extension rod is located the inside first limiting plate of one end fixedly connected with of depression bar, the inside of depression bar is seted up and is supplied the gliding first spacing groove of first limiting plate, the right side cell wall fixedly connected with second elastic component of first spacing groove, one end of second elastic component and one side fixed connection of first limiting plate, the extension rod extends to the inside one end of second spout and laminates each other with the top of first dysmorphism piece.

Preferably, first movable groove has all been seted up to the left and right sides cell wall of first spout, the inside sliding connection in first movable groove has second dysmorphism piece, one side of second dysmorphism piece supports tightly each other with one side of first dysmorphism piece, one side cell wall fixed connection of telescopic link and first movable groove is passed through to one side of second dysmorphism piece, the outside cover of telescopic link is equipped with the third elastic component, the both ends of third elastic component respectively with one side of second dysmorphism piece, one side cell wall fixed connection in first movable groove.

Preferably, elevating system includes the square post with the middle part fixed connection of depression bar, the gliding square groove of square post that supplies is seted up to the inside of base, square groove is linked together with the second spout, there is the threaded rod inside of square post through threaded connection, the top of threaded rod runs through square post, base and rotates with the base to be connected, the second activity groove has been seted up to the inside of base, the threaded rod extends to the inside one end of second activity inslot and rotates with the top cell wall of second activity groove to be connected.

Preferably, the bottom of the threaded rod is fixedly connected with a second limiting plate, and a second limiting groove for the second limiting plate to slide is formed in the square column.

Preferably, the outer side of the threaded rod is fixedly connected with a worm wheel, the outer side of the worm wheel is meshed with a worm, the left end of the worm is rotatably connected with the left side groove wall of the second movable groove, the bottom side groove wall of the second movable groove is fixedly provided with a motor, and the output shaft of the motor is fixedly connected with the right end of the worm.

Preferably, the inside fixedly connected with of base goes up the appearance pipe, the gliding sliding hole of confession appearance pipe is all seted up in the inside of depression bar, sliding plate, fixed plate, go up appearance pipe sliding fit in the inside of sliding hole, the top of going up the appearance pipe runs through the base and the top of going up the appearance pipe blocks up there is the stopper. Wherein, the stopper of the top jam of sample loading pipe passes through the top fixed connection of rope with the base to ensure that the stopper can not lose easily.

According to the technical scheme, the method has the following beneficial effects:

1: the in-process that the depression bar moved down makes the extension rod promote flourishing liquid box downstream earlier, drives the liquid outlet nozzle and inserts the drippage hole for the liquid outlet nozzle is close to and waits to add the model, thereby reduces the distance between waiting to add model and the liquid outlet nozzle, ensures the aseptic nature of sample injector.

2: second dysmorphism piece extrusion extension rod for the depression bar extrudees the sliding plate, thereby makes to produce the gap between trapezoidal sprue and the liquid outlet nozzle, thereby makes to add sample liquid from the gap between trapezoidal sprue and the liquid outlet nozzle drip to treat every downthehole of application of sample board, and then realizes the application of sample in batches, avoids the application of sample error.

3: the T-shaped clamping block slides in the T-shaped clamping groove in a clamping manner, so that the pressing rod can drive the liquid containing box to ascend or descend, and the device is convenient to use.

4: the motor starts for the worm rotates the drive worm wheel and rotates, makes square post slide along the threaded rod in square inslot downwards, thereby plays the purpose of the ascending decline of control depression bar, and then makes things convenient for the better application of sample of the device.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a schematic diagram of a 96-well plate sterile autosampler according to the present invention;

FIG. 2 is a schematic diagram of the internal structure of a base of a 96-well plate sterile autosampler according to the present invention;

FIG. 3 is an enlarged schematic view of the structure at A in FIG. 2 according to the present invention;

FIG. 4 is an enlarged schematic view of the structure at B in FIG. 2 according to the present invention;

FIG. 5 is a schematic diagram of a structure of a dripping well of a 96-well plate sterile autosampler according to the present invention;

FIG. 6 is a schematic diagram of the structure of a 96-well plate sterile autosampler wand according to the present invention.

Description of the drawings: 1. a base; 2. a plate to be loaded with sample; 3. a slot; 4. a liquid containing box; 5. a first chute; 6. a first shaped block; 7. a fixing plate; 8. a sliding plate; 9. a regulating and controlling rod; 10. a first elastic member; 11. a trapezoidal block; 12. a liquid outlet nozzle; 13. a liquid outlet; 14. a T-shaped fixture block; 15. a T-shaped clamping groove; 16. a pressure lever; 17. an extension rod; 18. a first limit plate; 19. a second elastic member; 20. a first limit groove; 21. a second shaped block; 22. a telescopic rod; 23. a third elastic member; 24. a first movable slot; 25. a square column; 26. a square groove; 27. a threaded rod; 28. a second limiting plate; 29. a second limit groove; 30. a worm gear; 31. a worm; 32. a motor; 33. a second movable slot; 34. a drip hole; 35. a second chute; 36. and (4) loading a sample tube.

Detailed Description

The following description is merely exemplary in nature and is not intended to limit the present disclosure, application, or uses. It should be understood that throughout the drawings, identical or similar reference numerals indicate identical or similar parts and features. The drawings are only schematic representations of the concepts and principles of the embodiments of the disclosure, and do not necessarily show specific dimensions or proportions of the various embodiments of the disclosure. Certain features that are part of a particular figure may be exaggerated in order to illustrate relevant details or structures of embodiments of the present disclosure.

Referring to FIGS. 1-6:

example one

The utility model provides a 96 orifice plate aseptic automatic sample injector, which comprises a base 1, one side sliding connection of base 1 remains to add the model 2, the supply is seted up in the inside of base 1 and is waited to add the gliding slot 3 of model 2, the inside sliding connection of base 1 has the flourishing liquid box 4 that is used for placing the appearance liquid, the gliding second spout 35 of confession flourishing liquid box 4 is seted up to the inside of base 1, multiunit dripping hole 34 has been seted up to the inside of base 1, dripping hole 34 and slot 3, second spout 35 is linked together, the inside of flourishing liquid box 4 is provided with and is used for controlling the control assembly that adds the appearance liquid outflow, the inside sliding connection of second spout 35 has depression bar 16, the inside of base 1 is provided with the elevating system who goes up and down to depression bar 16.

Specifically, the control assembly is including the sliding plate 8 with the left and right sides cell wall sliding connection of flourishing liquid box 4, the equal fixedly connected with T type slider in both sides about sliding plate 8, the gliding T type spout of confession T type slider has all been seted up to the left and right sides inner wall of flourishing liquid box 4, the left and right sides inner wall fixedly connected with fixed plate 7 of flourishing liquid box 4, the bottom fixedly connected with of sliding plate 8 and the corresponding multiunit regulation and control pole 9 of multiunit dropping hole 34, regulation and control pole 9 run through fixed plate 7 and with fixed plate 7 sliding connection, the outside cover of regulation and control pole 9 is equipped with first elastic component 10, the both ends of first elastic component 10 respectively with the bottom side of sliding plate 8, the top side fixed connection of fixed plate 7. It should be noted that the first elastic member 10 may be a spring, and in an original state, the outer side of the trapezoidal block 11 and the inner wall of the liquid outlet nozzle 12 can be tightly attached to each other.

More specifically, the bottom fixedly connected with multiunit drain nozzle 12 of flourishing liquid box 4, the liquid outlet 13 that is linked together with the inside of drain nozzle 12 is seted up to the bottom of flourishing liquid box 4, and regulation and control pole 9 runs through liquid outlet 13 and extends to the trapezoidal sprue 11 of the inside one end fixedly connected with of drain nozzle 12, and the outside of trapezoidal sprue 11 supports tightly each other with the inner wall of drain nozzle 12.

Further, the first dysmorphism piece 6 of all fixedly connected with in both sides about the liquid containing box 4 top, the gliding first spout 5 of confession first dysmorphism piece 6 is seted up to the inside of base 1, first spout 5 is linked together with second spout 35, T type draw-in groove 15 has all been seted up to one side that two sets of first dysmorphism pieces 6 are close to each other, the bottom side at both ends is all fixedly connected with T type fixture block 14 about 16 depression bars, T type fixture block 14 sliding fit in the inside of T type draw-in groove 15.

Furthermore, both ends all have sliding connection to have an extension rod 17 about depression bar 16, extension rod 17 is located the inside one end fixedly connected with first limiting plate 18 of depression bar 16, the inside of depression bar 16 is seted up and is supplied the gliding first spacing groove 20 of first limiting plate 18, the right side cell wall fixedly connected with second elastic component 19 of first spacing groove 20, one end and one side fixed connection of first limiting plate 18 of second elastic component 19, extension rod 17 extends to the inside one end of second spout 35 and laminates each other with the top of first dysmorphism piece 6. It is worth mentioning that the second elastic element 19 can be a spring, and in its original state, it can make one end of the extension rod 17 inside the second sliding slot 35 and abut against the top of the first profile block 6.

Preferably, first movable groove 24 has all been seted up to the left and right sides cell wall of first spout 5, the inside sliding connection of first movable groove 24 has second dysmorphism piece 21, one side of second dysmorphism piece 21 and one side of first dysmorphism piece 6 support tightly each other, one side of second dysmorphism piece 21 is passed through telescopic link 22 and one side cell wall fixed connection of first movable groove 24, the outside cover of telescopic link 22 is equipped with third elastic component 23, the both ends of third elastic component 23 respectively with one side of second dysmorphism piece 21, one side cell wall fixed connection of first movable groove 24. It should also be mentioned that the third elastic element 23 can be a spring and in its original state can cause the second shaped block 21 to hold the extension rod 17 so that it can slide between the two sets of first shaped blocks 6.

It is also worth mentioning that the inclined surface of the second special-shaped block 21 and the inclined surface of the first special-shaped block 6 are attached to each other, so that the second special-shaped block 21 can move along the inclined surface of the first special-shaped block 6, and the second special-shaped block 21 can conveniently extend out of the first movable groove 24 and return to the first movable groove 24 again when the first special-shaped block 6 ascends or descends.

Example two

The utility model provides an aseptic automatic sample injector of 96 orifice plates, it is on the basis of embodiment one, elevating system includes the square post 25 with the middle part fixed connection of depression bar 16, square groove 26 that supplies the square post 25 to slide is seted up to the inside of base 1, square groove 26 is linked together with second spout 35, there is threaded rod 27 square post 25's inside through threaded connection, square post 25 is run through on the top of threaded rod 27, base 1 just rotates with base 1 to be connected, second movable groove 33 has been seted up to the inside of base 1, threaded rod 27 extends to the inside one end of second movable groove 33 and is connected with the top cell wall rotation of second movable groove 33.

Specifically, the bottom of the threaded rod 27 is fixedly connected with a second limiting plate 28, and a second limiting groove 29 for allowing the second limiting plate 28 to slide is formed in the square column 25. It should be noted that, by providing the second limit plate 28, the square column 25 may not be completely moved out of the square groove 26 during the downward movement.

More specifically, a worm wheel 30 is fixedly connected to the outer side of the threaded rod 27, a worm 31 is meshed with the outer side of the worm wheel 30, the left end of the worm 31 is rotatably connected with the left side groove wall of a second movable groove 33, a motor 32 is fixedly mounted on the bottom side groove wall of the second movable groove 33, and the output shaft of the motor 32 is fixedly connected with the right end of the worm 31.

Further, a sample loading tube 36 is fixedly connected to the inside of the base 1, sliding holes for the sample loading tube 36 to slide are formed in the pressure lever 16, the sliding plate 8 and the fixing plate 7, the sample loading tube 36 is in sliding fit with the inside of the sliding holes, the top end of the sample loading tube 36 penetrates through the base 1, and a plug is plugged at the top of the sample loading tube 36.

The working principle is as follows: firstly, sample adding liquid is poured into the liquid containing box 4 from the top end of the sample adding pipe 36, after the sample adding is finished, the plug is plugged, then the prepared sample adding plate 2 to be added is inserted into the slot 3 in a sliding manner, then, sample adding can be carried out on the pore plate in the sample adding plate 2 to be added, when sample adding is needed, the motor 32 is started, the worm 31 is rotated to drive the worm wheel 30 to rotate, the threaded rod 27 is rotated, the square column 25 is driven to move downwards in the square slot 26 along the threaded rod 27, meanwhile, the second limit plate 28 also moves upwards in the second limit slot 29, the square column 25 drives the pressure rod 16 to move downwards in the second sliding slot 35, as the T-shaped clamping block 14 is clamped in the T-shaped clamping slot 15, the pressure rod 16 drives the extension rod 17 to press the first special-shaped block 6, the first special-shaped block 6 drives the liquid containing box 4 to move downwards, and the liquid outlet 12 moves downwards and passes through the dripping hole 34, thereby effectively reducing the time length of the sample adding liquid exposed outside;

when the first special-shaped block 6 moves to be completely separated from the second special-shaped block 21 and is not attached any more, the third elastic element 23 releases to drive the telescopic rod 22 to extend, so that the second special-shaped block 21 moves towards the extension rod 17 along the inclined surface of the first special-shaped block 6, then the second special-shaped block 21 begins to press the extension rod 17, the first limiting plate 18 moves in the first limiting groove 20, so that the second elastic element 19 is compressed, then the extension rod 17 is pressed by the first special-shaped block 6 in the process that the press rod 16 continues to descend, so that the press rod 16 is not blocked by the first special-shaped block 6 and begins to move downwards along the two groups of first special-shaped blocks 6, at the moment, the T-shaped clamping block 14 slides in the T-shaped clamping groove 15, and after the press rod 16 contacts the sliding plate 8 in the process of moving downwards, the sliding plate 8 is pushed to move downwards in the liquid containing box 4, so that the adjusting and controlling rod 9 is driven to move downwards, so that the first elastic element 10 is pressed, trapezoidal sprue 11 moves down in drain nozzle 12, and then makes trapezoidal sprue 11 and drain nozzle 12 no longer laminate, further makes the application of sample liquid flow out through liquid outlet 13 and through the inside that liquid outlet 12 and trapezoidal sprue between gap inflow drain nozzle 12, and finally the drippage is to treating every downthehole in application of sample board 2.

Exemplary embodiments of the proposed solution of the present disclosure have been described in detail above with reference to preferred embodiments, however, it will be understood by those skilled in the art that many variations and modifications may be made to the specific embodiments described above, and that many combinations of the various technical features, structures presented in the present disclosure may be made without departing from the concept of the present disclosure, without departing from the scope of the present disclosure, which is defined by the appended claims.

Claims

1. A96-well plate sterile automatic sample injector comprises a base (1) and is characterized in that, one side of the base (1) is connected with a sample plate (2) to be added in a sliding way, a slot (3) for the sample plate (2) to be added to slide is arranged in the base (1), the interior of the base (1) is connected with a liquid containing box (4) used for containing sample adding liquid in a sliding way, a second chute (35) for the liquid containing box (4) to slide is arranged in the base (1), a plurality of groups of dripping holes (34) are arranged inside the base (1), the dripping holes (34) are communicated with the slot (3) and the second sliding chute (35), a control component for controlling the outflow of the sample adding liquid is arranged in the liquid containing box (4), the inside sliding connection of second spout (35) has depression bar (16), the inside of base (1) is provided with the elevating system who goes up and down depression bar (16).

2. The sterile automatic sample injector with a 96-pore plate according to claim 1, characterized in that the control assembly comprises a sliding plate (8) slidably connected with the inner walls of the left and right sides of the liquid containing box (4), the left and right sides of the sliding plate (8) are fixedly connected with T-shaped sliding blocks, the inner walls of the left and right sides of the liquid containing box (4) are provided with T-shaped sliding grooves for the T-shaped sliding blocks to slide, the inner walls of the left and right sides of the liquid containing box (4) are fixedly connected with a fixed plate (7), the bottom of the sliding plate (8) is fixedly connected with a plurality of adjusting and controlling rods (9) corresponding to the plurality of dripping holes (34), the adjusting and controlling rods (9) penetrate through the fixed plate (7) and are slidably connected with the fixed plate (7), a first elastic element (10) is sleeved on the outer side of the adjusting and controlling rods (9), and two ends of the first elastic element (10) are respectively connected with the bottom side of the sliding plate (8), The top side of the fixing plate (7) is fixedly connected.

3. The sterile automatic sample injector with 96-pore plates as claimed in claim 2, characterized in that a plurality of groups of liquid outlets (12) are fixedly connected to the bottom of the liquid containing box (4), a liquid outlet (13) communicated with the inside of the liquid outlet (12) is formed in the bottom of the liquid containing box (4), the adjusting and controlling rod (9) penetrates through the liquid outlet (13) and extends to one end inside the liquid outlet (12) to be fixedly connected with a trapezoidal block (11), and the outer side of the trapezoidal block (11) is abutted against the inner wall of the liquid outlet (12).

4. The sterile automatic sample injector with 96-well plate according to claim 3, characterized in that the first special-shaped block (6) is fixedly connected to the left and right sides of the top of the liquid containing box (4), the first sliding groove (5) for the first special-shaped block (6) to slide is formed in the base (1), the first sliding groove (5) is communicated with the second sliding groove (35), the T-shaped clamping grooves (15) are formed in the sides of the two groups of first special-shaped blocks (6) close to each other, the T-shaped clamping blocks (14) are fixedly connected to the bottom sides of the left and right ends of the pressure lever (16), and the T-shaped clamping blocks (14) are slidably fitted in the T-shaped clamping grooves (15).

5. The sterile autosampler with 96-pore plate according to claim 4, characterized in that the left and right ends of the pressure lever (16) are slidably connected with an extension rod (17), the extension rod (17) is located at one end of the interior of the pressure lever (16) and fixedly connected with a first limit plate (18), the interior of the pressure lever (16) is provided with a first limit groove (20) for the first limit plate (18) to slide, the right groove wall of the first limit groove (20) is fixedly connected with a second elastic member (19), one end of the second elastic member (19) is fixedly connected with one side of the first limit plate (18), and one end of the extension rod (17) extending to the interior of the second sliding groove (35) is mutually attached to the top of the first special-shaped block (6).

6. The sterile automatic sample injector with 96 pore plates as claimed in claim 5, wherein the first movable groove (24) is formed in each of the left and right side groove walls of the first sliding groove (5), the second shaped block (21) is slidably connected to the inside of the first movable groove (24), one side of the second shaped block (21) and one side of the first shaped block (6) are abutted against each other, one side of the second shaped block (21) is fixedly connected to one side groove wall of the first movable groove (24) through a telescopic rod (22), a third elastic member (23) is sleeved on the outer side of the telescopic rod (22), and two ends of the third elastic member (23) are fixedly connected to one side of the second shaped block (21) and one side groove wall of the first movable groove (24) respectively.

7. The sterile autosampler with 96-well plate according to claim 1, characterized in that, the lifting mechanism comprises a square column (25) fixedly connected to the middle of the pressure bar (16), the inside of the base (1) is opened with a square groove (26) for the square column (25) to slide, the square groove (26) is communicated with the second sliding groove (35), the inside of the square column (25) is connected with a threaded rod (27) through a thread, the top end of the threaded rod (27) penetrates through the square column (25), the base (1) and is rotatably connected with the base (1), the inside of the base (1) is opened with a second movable groove (33), and one end of the threaded rod (27) extending to the inside of the second movable groove (33) is rotatably connected with the top groove wall of the second movable groove (33).

8. The sterile autosampler with 96-well plate according to claim 7, characterized in that the bottom of the threaded rod (27) is fixedly connected with a second limit plate (28), and the inside of the square column (25) is opened with a second limit groove (29) for the second limit plate (28) to slide.

9. The sterile autosampler with 96 pore plates according to claim 8, characterized in that the outer side of the threaded rod (27) is fixedly connected with a worm wheel (30), the outer side of the worm wheel (30) is engaged with a worm (31), the left end of the worm (31) is rotatably connected with the left side groove wall of the second movable groove (33), the bottom side groove wall of the second movable groove (33) is fixedly provided with a motor (32), and the output shaft of the motor (32) is fixedly connected with the right end of the worm (31).

10. The sterile autosampler with 96-well plate according to claim 2, characterized in that the base (1) is fixedly connected with a sample loading tube (36), the pressure bar (16), the sliding plate (8) and the fixing plate (7) are all provided with sliding holes for the sample loading tube (36) to slide, the sample loading tube (36) is in sliding fit with the sliding holes, the top end of the sample loading tube (36) penetrates through the base (1) and the top of the sample loading tube (36) is plugged with a plug.