CN114890551A - Tandem type biological denitrification method based on bacterial-algae symbiosis - Google Patents
Tandem type biological denitrification method based on bacterial-algae symbiosis Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 title claims abstract description 20
- 239000002351 wastewater Substances 0.000 claims abstract description 52
- 238000006243 chemical reaction Methods 0.000 claims abstract description 45
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 230000008569 process Effects 0.000 claims abstract description 18
- 230000001546 nitrifying effect Effects 0.000 claims abstract description 14
- 230000003647 oxidation Effects 0.000 claims abstract description 13
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 13
- 229910021529 ammonia Inorganic materials 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 65
- 229910052757 nitrogen Inorganic materials 0.000 claims description 22
- 241000195493 Cryptophyta Species 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 14
- 239000012528 membrane Substances 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 6
- 238000012856 packing Methods 0.000 claims description 5
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 3
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 241001453382 Nitrosomonadales Species 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims 8
- 239000000945 filler Substances 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 abstract description 11
- 239000010865 sewage Substances 0.000 abstract description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 10
- 239000001301 oxygen Substances 0.000 abstract description 10
- 238000005265 energy consumption Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 abstract description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 230000007423 decrease Effects 0.000 description 8
- 238000005273 aeration Methods 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 241000108664 Nitrobacteria Species 0.000 description 3
- 230000001651 autotrophic effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000001590 oxidative effect Effects 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
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- 230000036632 reaction speed Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/307—Nitrification and denitrification treatment characterised by direct conversion of nitrite to molecular nitrogen, e.g. by using the Anammox process
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention provides a serial biological denitrification method based on bacterial-algae symbiosis, which relates to the technical field of sewage treatment and aims to solve the problems of higher energy consumption and lower efficiency in the existing wastewater denitrification treatment process 2 ‑ -N, providing a reaction matrix for anammox in reactor No. two; culturing anaerobic ammonium oxidation bacteria in a second reactorAn anaerobic ammonia oxidation biomembrane system is constructed in the second reactor and is cultured separately from the shortcut nitrifying bacteria, so that the high dissolved oxygen condition required by shortcut nitrifying is avoided, the inhibition effect on anaerobic ammonia oxidation is realized, the efficiency of utilizing ammonia nitrogen by anaerobic ammonia oxidation is improved, and the high-efficiency denitrification is realized.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a serial biological denitrification method based on bacterial-algae symbiosis.
Background
Along with the continuous improvement of the living standard of people in China and the continuous improvement of a pipeline collecting system, the types and components of the generated sewage and wastewater become complicated, and the water quality of the inlet water of the sewage treatment plant generally has the characteristics of low organic matter content and high nitrogen and phosphorus content. However, the mainstream sewage treatment process commonly adopted by domestic sewage treatment plants has the problems of high energy consumption, high organic matter consumption and the like, and particularly has the problem that the total nitrogen of the effluent is difficult to reach the standard when the sewage with low C/N ratio is treated, so that a novel low-energy-consumption high-efficiency biological denitrification device and method are urgently needed in China to meet the development requirements of the economic society of China.
Anammox is a biological denitrification technique emerging in recent years, and is to use anammox bacteria to remove NH under anaerobic conditions 4 + -N and NO 2 － Conversion of-N to N 2 The denitrification process. Compared with the traditional nitrification-denitrification process, the anaerobic ammonia oxidation process reduces aeration consumption (60 percent), organic carbon requirement (100 percent), sludge yield (90 percent) and greenhouse gas emission (N) 2 O and CO 2 ). Although anammox offers so many advantages over the traditional process, it also has certain limitations. Since nitrogen in sewage is usually NH 4 + -N form, absent substrate NO for anammox reaction 2 － and-N, so it is difficult to directly utilize anammox. The existing method is to add partial NH in water body for advanced treatment by using shortcut nitrifying bacteria 4 + Oxidation of-N to NO 2 － -N followed by anammox. However, the short-cut nitrification requires a large amount of aeration and a large amount of energy. With the continuous promotion of the policy of 'double carbon' in China, the reduction of energy consumption becomes the direction in the water treatment industry.
The bacteria-algae symbiotic system is a sewage treatment technology combining bacteria and microalgae, and is widely concerned by researchers in recent years. Based on the resource-friendly development concept, the symbiosis and the novel removal of the bacteria and the algae are realizedCombining nitrogen process, adopting biomembrane method to couple microalgae and short-range nitrifying bacteria into integrated biomembrane system to form autotrophic biomembrane system with symbiosis of bacteria and algae, wherein the microalgae in the system provides oxygen for the short-range nitrifying bacteria to utilize through photosynthesis, and further NH in water is carried out under the action of the short-range nitrifying bacteria 4 + Oxidation of-N to NO 2 － And N, the biological membrane system can ensure biomass, does not need aeration, greatly reduces aeration energy consumption and treatment cost, can provide a reaction substrate for subsequent anaerobic ammonia oxidation, promotes smooth reaction, and finally realizes low-energy-consumption efficient denitrification of sewage through a second anaerobic ammonia oxidation biological membrane system. Therefore, the novel biological denitrification technology based on the symbiosis of the bacteria and the algae has better application prospect in the aspect of sewage denitrification.
Disclosure of Invention
In view of the problems in the prior art, the invention discloses a serial connection type biological denitrification method based on the symbiosis of bacteria and algae, which adopts the technical scheme that the method comprises the following steps,
step 1, leading the NH with the concentration of 90-110mg/L in a water inlet tank 4 + N waste water enters a reactor I through a water inlet pipe I, and a stirring device in the reactor I is started to stir the waste water; open lighting device simultaneously, lighting device includes a plurality of banks, reactor SBBR sequencing batch biofilm reactor, and reactor adopts transparent material, little algae of biofilm layer can be under the illumination condition on the material frame in reactor, thereby produce oxygen through photosynthesis and improve aquatic dissolved oxygen concentration, promote the shortcut nitrobacteria of biofilm inlayer to take place the shortcut nitrification reaction, little algae can adopt the chlorella, utilize the oxygen that little algae produced, provide the shortcut nitrobacteria, make the shortcut nitrobacteria with NH 4 + Conversion of-N to NO 2 - N, conversion 100%, conversion equation NH 4 + +1.5O 2 →NO 2 - +H 2 O+2H + ；
step 4, adding NH with the concentration of 90-110mg/L into the water inlet tank 4 + Directly conveying the N wastewater into an intermediate water tank through a clean water No. 2 pipe and a water inlet No. two pump, and uniformly mixing the N wastewater with the effluent of the reactor No. one;
and 7, increasing the pH value in the second reactor from 7.5-7.9 to 8.0-8.4, gradually increasing the pH value, closing the stirring device when the pH value does not rise any more and slightly falls, and then reacting a substrate NH in the second reactor 4 + -N and NO 2 - N is completely consumed, and the reaction of the second reactor is finished
And 8, after the reaction of the second reactor is finished, opening the water outlet device, and conveying a water outlet in the second reactor to a water outlet tank through a water outlet pump to finish denitrification treatment.
The invention has the beneficial effects that: the invention couples short-cut nitrifying bacteria and autotrophic nitrogen removal microorganisms such as microalgae and the like, constructs a bacteria-algae symbiotic system in a reactor I, generates oxygen by the photosynthesis of the microalgae at the outer layer of a biomembrane in the reactor I, and realizes short-cut nitrification by the short-cut nitrifying bacteria at the inner layer of the biomembrane by utilizing the oxygen in water, namely NH 4 + Oxidation of-N to NO 2 - -N, NO aeration equipment is needed in the process, the aeration energy consumption is saved, and NO generated by short-cut nitrification 2 - N, providing a reaction substrate for anammox in reactor No. two.
Furthermore, anaerobic ammonia oxidizing bacteria are cultured in the second reactor, an anaerobic ammonia oxidizing biomembrane system is constructed in the second reactor and is cultured separately from the shortcut nitrifying bacteria, so that the high dissolved oxygen condition required by shortcut nitrifying is avoided, the inhibition effect on anaerobic ammonia oxidizing is improved, the efficiency of utilizing ammonia nitrogen by anaerobic ammonia oxidizing is improved, and efficient denitrification is realized.
Furthermore, the two constructed biological membrane systems are autotrophic biological membrane systems, no additional carbon source is needed, energy is saved, efficiency is high, the transformation requirements of 'double carbon' and green water plants in China are met, and the two constructed biological membrane systems have great utilization value.
The lamp group is adopted to provide a light source for the microalgae on the outer layer of the biological membrane in the reactor I, so that the oxygen production rate of the microalgae is improved; meanwhile, when the biological membrane is illuminated, the photothermal effect provides a more appropriate reaction temperature for the microorganisms, so that the reaction activity of the microorganisms is improved, the reaction speed is accelerated, and the treatment cost is further reduced.
Drawings
In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.
FIG. 1 is a schematic view of the structure of the present invention.
In the figure: the system comprises a first reactor-1, a filling frame 2, a second reactor-3, a second filling frame-4, a water inlet tank-5, a first water inlet pipe-6, a first water inlet pump-7, a second water inlet pipe-8, a second water inlet pump-9, a first connecting pipe-11, a second connecting pipe-13, a first connecting pump-10, a second connecting pump-14, an intermediate water tank-12, a water outlet pipe-16, a water outlet tank-17 and a water outlet pump-15.
Detailed Description
The technical scheme of the invention is clearly and completely described in the following with reference to the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Moreover, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1
As shown in figure 1, the invention discloses a tandem type biological denitrification method based on bacterial-algae symbiosis, which adopts the technical scheme that the method comprises the following steps:
step 1, adding NH with a certain concentration in a water inlet tank 5 4 + Introducing the N wastewater into a first reactor 1,stirring the wastewater in the first reactor 1, and illuminating the first reactor 1;
step 4, adding NH into the water inlet tank 5 4 + N of the remaining waste water is sent to the intermediate tank 12;
And 8, after the reaction of the second reactor 3 is finished, conveying the wastewater in the second reactor 3 into a water outlet tank 17 to finish denitrification.
As a preferred technical scheme of the invention, in the step 1, NH with the concentration of 90mg/L is introduced into the first reactor 1 4 + -N waste water.
As a preferred technical scheme of the invention, in the step 2, NH is adopted 4 + Conversion of-N to NO 2 — The chemical reaction equation of N is NH 4 + +1.5O 2 →NO 2 - +H 2 O+2H + 。
In a preferred embodiment of the present invention, in step 2, the PH of the wastewater gradually decreases from 8.0, and when the PH does not decrease any more and the PH increases, the PH of the wastewater is 7.3.
As a preferred technical solution of the present invention, in the step 5, NH is in the water in the intermediate water tank 12 4 + -N and NO 2 - -N content ratio of 1: 1.32.
as a preferred embodiment of the present invention, in the step 6, NH is performed 4 + -N and NO 2 - Conversion of-N to N 2 The chemical reaction equation of (A) is NH 4 + +1.32NO 2 - +0.066HCO 3 - +0.13H + →1.02N 2 +0.26NO 3 -
+0.066CH 2 O 0.5 N 0.15 +2.03H 2 0。
In a preferred embodiment of the present invention, in step 7, the PH of the wastewater gradually increases from 7.5, and when the PH does not increase any more and decreases, the PH of the wastewater is 8.0.
As a preferred technical scheme of the invention, the first reactor 1 and the second reactor 3 both adopt SBBR sequencing batch biofilm reactors.
In a preferred technical scheme of the invention, the microalgae in the packing frame 2 in the reactor 1 is chlorella.
In a preferred embodiment of the present invention, the anaerobic ammonium oxidation bacteria are cultured on the second packing rack 4 in the second reactor 3.
Example 2
This example differs from example 1 in that: in the step 1, NH with the concentration of 110mg/L is introduced into the first reactor 1 4 + -N waste water.
In a preferred embodiment of the present invention, in step 2, the PH of the wastewater gradually decreases from 7.4, and when the PH does not decrease any more and the PH increases, the PH of the wastewater is 7.7.
In a preferred embodiment of the present invention, in step 7, the PH of the wastewater gradually rises from 7.9, and when the PH does not rise any more and falls, the PH of the wastewater is 8.4.
Example 3
This example differs from example 1 in that: in the step 1, NH with the concentration of 100mg/L is introduced into the first reactor 1 4 + -N waste water.
In a preferred embodiment of the present invention, in step 2, the PH of the wastewater gradually decreases from 7.7, and when the PH does not decrease any more and the PH increases, the PH of the wastewater is 7.5.
In a preferred embodiment of the present invention, in step 7, the PH of the wastewater gradually increases from 7.7, and when the PH does not increase any more and decreases, the PH of the wastewater is 8.2.
The working principle of the invention is as follows: the effective volumes of the first reactor 1 and the second reactor 3 are 14L, and NH is arranged in the water inlet tank 5 4 + Simulated NH content at an N concentration of 100mg/L 4 + N waste water enters the first reactor 1 under the power of a water inlet first pump I3, the pH of the waste water in the first reactor 1 is adjusted to about 8.2 before the reaction starts, the power of each group of lamps in the lamp group is 50W, the distance from the outer wall of the reactor is 10cm-15cm, and a pH meter on the first reactor 1 is used for detecting and recording the reaction state in the first reactor 1; when the PH value of the wastewater in the first reactor 1 is stable and does not fall any more, the lamp bank is turned off and the stirring is stopped, the wastewater in the first reactor 1 enters the middle water tank 12 under the power of the first pump 10, and then the second water inlet pump 9 is turned on to enable a part of the water inlet tank 5 to contain NH 4 + N waste water is conveyed to the intermediate tank 12; then, after the water in the middle water tank 12 is uniformly mixed, the water is conveyed into the second reactor 3 through the second pump 14; the PH value of the wastewater is adjusted to about 7.8 before and after the second reactor 3 starts to react, and a PH meter on the second reactor 3 is used for detecting and recording the reaction state in the second reactor 3; when the second number is reversedWhen the pH value in the reactor 3 does not rise any more, the stirring in the reactor 3 II is stopped, and the reactor 3 II is discharged into the water outlet tank 17 after settling for half an hour.
In the denitrification process in the prior art, the denitrification rate of the wastewater is 83-89%, the biological denitrification of the symbiosis of bacteria and algae is carried out based on the denitrification process, the denitrification rate is 85-90%, the total nitrogen content in the wastewater before denitrification is 90-110mg/L, and the total nitrogen content in the wastewater after denitrification is 8.0-16.0 mg/L.
Components not described in detail herein are prior art.
Although the present invention has been described in detail with reference to the specific embodiments, the present invention is not limited to the above embodiments, and various changes and modifications without inventive changes may be made within the knowledge of those skilled in the art without departing from the spirit of the present invention.
Claims (10)
1. A tandem type biological denitrification method based on bacterial-algae symbiosis is characterized by comprising the following steps:
step 1, adding NH with a certain concentration in a water inlet tank (5) 4 + -N wastewater is passed into reactor No. 1, the wastewater in reactor No. 1 is stirred, and reactor No. 1 is illuminated;
step 2, after the pH value in the first reactor (1) is stable, the first reactor (1) is closed to be illuminated, stirring is stopped, and NH in the wastewater is treated by microalgae and shortcut nitrifying bacteria in a packing frame (2) in the first reactor (1) 4 + Conversion of all of-N to NO 2 - N, the reaction in reactor No. one (1) is finished;
step 3, conveying the wastewater in the first reactor (1) to an intermediate water tank (12);
step 4, the water inlet tank (5) contains NH 4 + -N of the remaining waste water is conveyed to an intermediate tank (12);
step 5, controlling and connecting the total flow in the first pipe (11) and the total flow in the second water inlet pipe (8) by controlling and connecting the operating power and time of the first pump (10) and the second water inlet pump (9)Amount of NH in the water in the intermediate tank (12) 4 + -N and NO 2 - -the N content ratio is maintained at a certain ratio;
step 6, conveying the wastewater in the intermediate water tank (12) into a second reactor (3), stirring the wastewater in the second reactor (3), and enabling NH in the water to pass through an anaerobic ammonia oxidation biological membrane on a second filling frame (4) in the second reactor (3) 4 + -N and NO 2 - Conversion of-N to N 2 ；
Step 7, after the pH value in the second reactor (3) is stable, the stirring in the second reactor (3) is closed, and at the moment, a reaction substrate NH in the second reactor (3) 4 + -N and NO 2 - And N is completely consumed, and the reaction of the second reaction device is finished.
And 8, after the reaction of the second reactor (3) is finished, conveying the wastewater in the second reactor (3) to a water outlet tank (17) to finish denitrification.
2. The in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: in the step 1, NH with the concentration of 90-110mg/L is introduced into the first reactor (1) 4 + -N waste water.
3. The in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: in said step 2, NH 4 + Conversion of-N to NO 2 — The chemical reaction equation of-N is NH 4 + +1.5O 2 →NO 2 - +H 2 O+2H + 。
4. The in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: in the step 2, the pH value of the wastewater is gradually reduced from 8.0-7.4, and when the pH value is not reduced any more and the pH value is increased, the pH value of the wastewater is 7.3-7.7.
5. A series connection according to claim 1The biological denitrification method based on the symbiosis of bacteria and algae is characterized in that: in the step 5, NH in the water in the intermediate water tank (12) 4 + -N and NO 2 - -N content ratio of 1: 1.32.
6. the in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: in said step 6, NH 4 + -N and NO 2 - Conversion of-N to N 2 The chemical reaction equation of (A) is NH 4 + +1.32NO 2 - +0.066HCO 3 - +0.13H + →1.02N 2 +0.26NO 3 - +0.066CH 2 O 0.5 N 0.15 +2.03H 2 0。
7. The in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: in the step 7, the pH value of the wastewater gradually rises from 7.5 to 7.9, and when the pH value does not rise any more and falls, the pH value of the wastewater is 8.0 to 8.4.
8. The in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: the first reactor (1) and the second reactor (3) both adopt SBBR sequencing batch biofilm reactors.
9. The in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: microalgae in the filler frame (2) in the first reactor (1) is chlorella.
10. The in-line bacterial algae symbiosis based biological denitrification process as claimed in claim 1, wherein: anaerobic ammonia oxidizing bacteria are cultured on a second filling frame (4) in the second reactor (3).
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| CN115259386A (en) * | 2022-08-19 | 2022-11-01 | 济南大学 | System and method for treating wastewater by using oligodynamic bacteria and algae through completely autotrophic biomembrane |
| CN115571982A (en) * | 2022-10-08 | 2023-01-06 | 中国电建集团华东勘测设计研究院有限公司 | Biological denitrification method of bacteria-algae symbiotic system based on pH and DO control |
| CN116040810A (en) * | 2023-03-03 | 2023-05-02 | 北京工业大学 | Sewage treatment system and sewage treatment method |
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