CN114878842A - Whole embryo immunohistochemical experiment container of zebra fish - Google Patents
Whole embryo immunohistochemical experiment container of zebra fish Download PDFInfo
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- CN114878842A CN114878842A CN202210455150.6A CN202210455150A CN114878842A CN 114878842 A CN114878842 A CN 114878842A CN 202210455150 A CN202210455150 A CN 202210455150A CN 114878842 A CN114878842 A CN 114878842A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a zebra fish whole embryo immunohistochemical experiment container which comprises a box body, a box cover, an accommodating pipe and a controller, wherein a plurality of reagent pipes are arranged in the box body, and each reagent pipe is used for accommodating a dyeing reagent; a driving structure is arranged on one side of the box cover facing the box body, the driving structure is detachably connected with the accommodating tube, and the driving structure is used for driving the accommodating tube to move; a liquid hole is formed in the bottom of the accommodating tube, and the accommodating tube is used for accommodating the zebra fish whole embryo; the controller is electrically connected with the driving structure and is used for controlling the driving structure to drive the accommodating pipes so that the accommodating pipes sequentially enter each reagent pipe to operate. According to the technical scheme provided by the invention, the accommodating tubes are controlled by the driving structure to sequentially enter the reagent tubes for dyeing operation, and the dyeing time and sequence are controlled by the controller, so that the full automation of an immunohistochemical experiment is realized, the experiment difficulty is reduced, and the working intensity of workers is reduced.
Description
Technical Field
The invention relates to the technical field of experimental instruments, in particular to a zebra fish whole embryo immunohistochemical experimental container.
Background
The live zebra fish screening model has a plurality of advantages, and overcomes the defects of the original in vitro model in the verification of absorption, distribution, metabolism and excretion links and the defects of long experimental period, complex operation and high cost of the traditional in vivo screening model. The zebra fish is a vertebral animal, the similarity of the zebra fish and human genes is as high as about 85%, and the experimental result is strong in comparability. Compared with mammals such as rats, the zebra fish whole embryo is transparent, can observe and analyze a plurality of organs simultaneously, and has the advantages of short experimental period, large sample capacity, high result reliability and low required cost. More importantly, the administration mode is simple, and the small molecular substances dissolved in water can directly enter the zebra fish body through the skin, gills and digestive system. In the process of carrying out the immunohistochemical experiment on the zebra fish whole embryo (the zebra fish whole embryo with the size of about 0.9-2.9mm), a dyeing reagent needs to be repeatedly replaced to soak the whole embryo, the single soaking time is short (about 30s-5min), so that the whole embryo needs to be repeatedly moved or the reagent needs to be replaced, the moving process is troublesome and takes long time, and the whole operation process needs to be operated by a worker in a laboratory for a long time, so that a large amount of working time of the worker is occupied.
Disclosure of Invention
The invention mainly aims to provide a zebra fish whole embryo immunohistochemical experiment container, and aims to solve the problems that the experiment process is complex and a large amount of working time needs to be occupied by workers.
In order to realize the purpose, the technical scheme provided by the invention is as follows:
a zebra fish whole embryo immunohistochemical experiment container comprises a box body, a box cover, an accommodating pipe and a controller, wherein a plurality of reagent pipes are arranged in the box body, and each reagent pipe is used for accommodating a dyeing reagent; a driving structure is arranged on one side, facing the box body, of the box cover, the driving structure is detachably connected with the accommodating tube, and the driving structure is used for driving the accommodating tube to move; the bottom of the accommodating tube is provided with a liquid hole, and the accommodating tube is used for accommodating the zebra fish whole embryo; the controller is electrically connected with the driving structure and used for controlling the driving structure to drive the accommodating tubes so that the accommodating tubes sequentially enter the reagent tubes to operate.
Preferably, each of said reagent tubes constitutes at least one row of well groups; the driving structure comprises at least one track, and the extending direction of the track is parallel to the arrangement direction of the hole groups; the rail is provided with an electric control sliding seat in a sliding manner, one side of the electric control sliding seat facing the hole group is provided with a lifter, and the electric control sliding seat is used for driving the lifter to slide along the rail; the lifting end of the lifter is detachably connected with the accommodating tube, and the lifter is used for driving the accommodating tube to enter and exit the reagent tube; the controller is electrically connected with the electric control sliding seat and the lifter respectively, and is used for controlling the electric control sliding seat and the lifter respectively so that the accommodating pipes sequentially enter the reagent pipes in the hole group to operate.
Preferably, the lifting end of the lifter is provided with an installation seat, one side of the installation seat facing the hole group is provided with a connecting column, and one end of the connecting column far away from the installation seat is provided with an external thread; the holding pipe is provided with an internal thread at one end far away from the liquid hole, and the holding pipe is in threaded connection with the connecting column.
Preferably, one end of the connecting column, which is far away from the mounting seat, is open, a camera is arranged in the connecting column, the camera is electrically connected with the controller, and the camera is used for shooting an operation image of the accommodating tube entering the reagent tube and sending the operation image to the controller; and a transparent water-stop sheet is arranged at the opening of the connecting column.
Preferably, the bottom of the accommodating tube is provided with a partition plate, the partition plate comprises a first transverse plate and a second transverse plate which are arranged in a crossed manner, the first transverse plate is perpendicular to the second transverse plate, and the first transverse plate and the second transverse plate are perpendicular to the bottom of the accommodating tube respectively.
Preferably, a fixed plate and two supporting plates are respectively arranged in the box body, one of the supporting plates is fixedly arranged on one side in the box body, and the other supporting plate is fixedly arranged on the other side in the box body; the fixed plates are horizontally arranged in the box body, one of the supporting plates supports one end of each fixed plate, the other supporting plate supports the other end of each fixed plate, and the two fixed plates are used for enabling the fixed plates to be suspended in the box body; one side of the fixing plate facing the box cover forms an operation space in the box body, and the operation space is used for the accommodating tube to move; each reagent tube is fixed to the fixing plate.
Preferably, one side of each supporting plate facing the box cover is provided with a limiting block respectively; limiting through holes are formed in two ends of the fixing plate respectively, one limiting block is inserted into one limiting through hole, and the other limiting block is inserted into the other limiting through hole.
Preferably, a liquid discharge pipe is arranged at one end of the reagent pipe, which is far away from the box cover, one end of the liquid discharge pipe is communicated with the reagent pipe, and a sealing cover is arranged at the other end of the liquid discharge pipe; the sealing cover is provided with a connecting hole at one end facing the liquid discharge pipe, an internal thread is arranged in the connecting hole, an external thread is arranged at one end of the liquid discharge pipe far away from the fixed pipe, and the sealing cover is in threaded connection with the liquid discharge pipe.
Preferably, a constant temperature space is formed in the box body on one side of the fixing plate, which is away from the operation space, and constant temperature liquid is stored in the constant temperature space, so that each reagent tube extending into the constant temperature space is kept in a preset temperature range; a temperature detector is arranged in the constant-temperature space, the temperature detector is electrically connected with the controller, and the temperature detector is used for detecting temperature data of constant-temperature liquid and sending the temperature data to the controller; the temperature control device is arranged outside the box body, a heating pipe and a cooling pipe are respectively arranged in the constant temperature space, the heating pipe and the cooling pipe respectively penetrate through the box body to be connected with the temperature control device, and the temperature control device is used for controlling the temperature of the constant temperature liquid through the heating pipe and the cooling pipe; the controller is electrically connected with the temperature control equipment, and the controller is used for controlling the temperature control equipment according to the temperature data.
Compared with the prior art, the invention at least has the following beneficial effects:
hold the intraductal dyeing operation that gets into each reagent in proper order through drive structure control to time and order through controller control dyeing have realized the full automatization of immunohistochemical experiment, have reduced the degree of difficulty of experiment, and staff's working strength.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of a zebra fish whole embryo immunohistochemical experimental container according to the present invention;
FIG. 2 is an enlarged view of the structure at A in FIG. 1;
fig. 3 is a schematic structural diagram of the box body in a top view.
The reference numbers illustrate:
1-box body; 11-a box cover; 12-a control panel; 13-a support plate; 14-a limiting block; 15-a working space; 16-a constant temperature space; 17-heating the tube; 18-a cooling tube;
2-accommodating a tube; 21-a separator;
3, fixing a plate; 31-a reagent tube; 32-a limiting through hole; 33-a drain pipe; 34-a sealing cover;
4-a drive configuration; 41-track; 42-an electrically controlled slide; 43-a lifter; 44-a mounting seat; 45-connecting column; 46-a micro motor;
the implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "connected," "secured," and the like are to be construed broadly, and for example, "secured" may be a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In addition, the technical solutions in the embodiments of the present invention may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination of technical solutions should not be considered to exist, and is not within the protection scope of the present invention.
The invention provides a zebra fish whole embryo immunohistochemical experiment container.
As shown in fig. 1 to 3, the container for the zebra fish whole embryo immunohistochemical experiment comprises a box body 1, a box cover 11, a containing tube 2 and a controller (not shown in the figure), wherein a plurality of reagent tubes 31 are arranged in the box body 1, and each reagent tube 31 is used for containing a staining reagent; a driving structure 4 is arranged on one side of the box cover 11 facing the box body 1, the driving structure 4 is detachably connected with the accommodating tube 2, and the driving structure 4 is used for driving the accommodating tube 2 to move; a liquid hole (not shown in the figure) is formed in the bottom of the accommodating tube 2, and the accommodating tube 2 is used for accommodating the zebra fish whole embryo; the controller is electrically connected with the driving structure 4, and is used for controlling the driving structure 4 to drive the accommodating tube 2 so that the accommodating tube 2 sequentially enters each reagent tube 31 for operation.
Hold pipe 2 and get into each reagent pipe 31 in proper order through drive structure 4 control and carry out the dyeing operation to time and order through controller control dyeing have realized the full automatization of immunohistochemical experiment, have reduced the degree of difficulty of experiment, and staff's working strength.
Specifically, a control panel 12 is disposed on a side of the box cover 11 away from the box body 1, the control panel 12 is electrically connected to the controller, and the control panel 12 is configured to send a control command to the controller, so that the controller controls the operation time and the operation sequence of the driving structure 4 to drive the accommodating tube 2 into a reagent tube 31.
Specifically, the diameter of the whole zebra fish embryo is about 0.9mm-2.9mm, and the aperture of the liquid hole is 30 meshes-40 meshes. Thus, the whole zebra fish embryo is prevented from leaking out of the liquid hole, and the reagent is ensured to rapidly enter the accommodating tube 2.
Each reagent tube 31 constitutes at least one row of well groups; the driving structure 4 comprises at least one track 41, the extension direction of the track 41 being parallel to the arrangement direction of the hole groups; an electric control sliding seat 42 is arranged on the rail 41 in a sliding mode, a lifter 43 is arranged on one side, facing the hole group, of the electric control sliding seat 42, and the electric control sliding seat 42 is used for driving the lifter 43 to slide along the rail 41; the lifting end of the lifter 43 is detachably connected with the accommodating tube 2, and the lifter 43 is used for driving the accommodating tube 2 to move in and out of the reagent tube 31; the controller is electrically connected to the electrically controlled slide 42 and the lifter 43, and the controller is used for controlling the electrically controlled slide 42 and the lifter 43, respectively, so that the accommodating tube 2 sequentially enters each reagent tube 31 in the hole group for operation.
Specifically, the number of hole groups is equal to the number of tracks 41.
Specifically, the hole group includes eight reagent tubes 31, and at least two groups are provided in the cartridge body 1.
The lifting end of the lifter 43 is provided with an installation seat 44, one side of the installation seat 44 facing the hole group is provided with a connecting column 45, and one end of the connecting column 45 far away from the installation seat 44 is provided with an external thread; the end, far away from the liquid hole, of the accommodating pipe 2 is provided with an internal thread, and the accommodating pipe 2 is in threaded connection with the connecting column 45.
Specifically, the lifting end of the lifter 43 is provided with a micro motor 46, the output end of the micro motor 46 is connected to the mounting seat 44, and the micro motor 46 is used for driving the mounting seat 44 to rotate, so that the accommodating tube 2 rotates when entering the reagent tube 31. Thus ensuring the full contact between the whole zebra fish embryo and the reagent.
One end of the connecting column 45, which is far away from the mounting seat 44, is open, a camera is arranged in the connecting column 45 and electrically connected with the controller, and the camera is used for shooting an operation image of the accommodating tube 2 entering the reagent tube 31 and sending the operation image to the controller; a transparent water-stop sheet (not shown) is provided at the opening of the connecting column 45. The camera is arranged to record the reaction process of the whole zebra fish embryo and leave detailed experimental records.
Specifically, the controller is also used for marking the abnormal zebra fish whole embryo in the operation image and forming a rapid experiment image of the abnormal zebra fish whole embryo; the control panel 12 includes a display screen (not shown) for displaying the working image and the rapid experiment image, respectively. The abnormal zebra fish whole embryo is the zebra fish whole embryo with abnormal color in the same batch (strong or good color). The fast experimental image is an image (for example, 10 times or 20 times) with multiple playing speeds of the abnormal whole zebra fish embryo in the operation image, so that a worker can conveniently and fast observe the abnormal whole zebra fish embryo.
The bottom of the tube containing tube 2 is provided with a partition plate 21, the partition plate 21 comprises a first transverse plate and a second transverse plate which are arranged in a crossed mode, the first transverse plate is perpendicular to the second transverse plate, and the first transverse plate and the second transverse plate are perpendicular to the bottom of the tube containing tube 2 respectively. The partition 21 is provided to divide the inside of the accommodating tube 2 into a plurality of regions for observation.
Specifically, the partition 21 and the accommodating tube 2 are detachably connected.
The box body 1 is internally provided with a fixed plate 3 and two supporting plates 13 respectively, wherein one supporting plate 13 is fixedly arranged at one side in the box body 1, and the other supporting plate 13 is fixedly arranged at the other side in the box body 1; the fixing plates 3 are horizontally arranged in the box body 1, one supporting plate 13 supports one end of the fixing plate 3, the other supporting plate 13 supports the other end of the fixing plate 3, and the two fixing plates 3 are used for enabling the fixing plate 3 to be suspended in the box body 1; the side of the fixing plate 3 facing the box cover 11 forms a working space 15 in the box body 1, and the working space 15 is used for moving the accommodating tube 2; the reagent tubes 31 are fixed to the fixing plate 3. The arrangement of the fixing plate 3 and the support plate 13 allows each reagent vessel 31 to be suspended, thus avoiding the reagent vessel 31 as a support and leaving a working space 15 for accommodating the tube 2 to enter and exit the reagent vessel 31.
Specifically, one side of the fixing plate 3 departing from the supporting plate 13 is provided with a handle, so that the fixing plate 3 can be conveniently taken down by a worker.
Limiting blocks 14 are respectively arranged on one side of each supporting plate 13 facing the box cover 11; the two ends of the fixing plate 3 are respectively provided with a limiting through hole 32, one limiting block 14 is inserted into one limiting through hole 32, and the other limiting block 14 is inserted into the other limiting through hole 32. The arrangement of the limiting block 14 and the limiting through hole 32 can facilitate the fixing plate 3 to be accurately hung on the two supporting plates 13.
Specifically, the outer edge of the limiting block 14 is provided with an elastic layer, so that the limiting block 14 and the limiting through hole 32 can be tightly connected.
A liquid discharge pipe 33 is arranged at one end of the reagent pipe 31 far away from the box cover 11, one end of the liquid discharge pipe 33 is communicated with the reagent pipe 31, and a sealing cover 34 is arranged at the other end of the liquid discharge pipe 33; one end of the sealing cover 34 facing the liquid discharge pipe 33 is provided with a connecting hole, an internal thread is arranged in the connecting hole, one end of the liquid discharge pipe 33 far away from the fixed pipe is provided with an external thread, and the sealing cover 34 is in threaded connection with the liquid discharge pipe 33. The provision of the drain tube 33 and the sealing cap 34 may facilitate the user to replace the reagents in the individual reagent tubes 31.
Specifically, the reagent tube 31 and the fixing plate 3 are integrally formed, so that the unstable connection between the reagent tube 31 and the fixing plate 3 can be prevented from affecting the entrance and exit of the accommodating tube 2. Avoiding a slight collision causes both the reagent tubes 31 to become inclined.
A constant temperature space 16 is formed in the box body 1 at one side of the fixing plate 3, which is far away from the operation space 15, and constant temperature liquid is stored in the constant temperature space 16, so that each reagent tube 31 extending into the constant temperature space 16 is kept in a preset temperature range; a temperature detector is arranged in the constant temperature space 16 and electrically connected with the controller, and the temperature detector is used for detecting temperature data of constant temperature liquid and sending the temperature data to the controller; the temperature control device is arranged outside the box body 1, the heating pipe 17 and the cooling pipe 18 are respectively arranged in the constant temperature space 16, the heating pipe 17 and the cooling pipe 18 respectively penetrate through the box body 1 to be connected with the temperature control device, and the temperature control device is used for controlling the temperature of constant temperature liquid through the heating pipe 17 and the cooling pipe 18; the controller is electrically connected with the temperature control equipment and is used for controlling the temperature control equipment according to the temperature data. The temperature regulation of whole experimentation can be guaranteed in the setting of constant temperature space 16, and the temperature control scope is 25 degrees centigrade to 70 degrees centigrade in the immunohistochemical experimentation, and needs the long-term temperature of guaranteeing when the operation of single reagent pipe 31, so thermostatic equipment can reduce the reliance to outside equipment.
Specifically, the temperature detector and the temperature control device are common devices in the prior art, and therefore are not described herein in detail.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (9)
1. The zebra fish whole embryo immunohistochemical experiment container is characterized by comprising a box body, a box cover, an accommodating pipe and a controller, wherein a plurality of reagent pipes are arranged in the box body, and each reagent pipe is used for accommodating a dyeing reagent; a driving structure is arranged on one side, facing the box body, of the box cover, the driving structure is detachably connected with the accommodating tube, and the driving structure is used for driving the accommodating tube to move; the bottom of the accommodating tube is provided with a liquid hole, and the accommodating tube is used for accommodating the zebra fish whole embryo; the controller is electrically connected with the driving structure and used for controlling the driving structure to drive the accommodating tubes so that the accommodating tubes sequentially enter the reagent tubes to operate.
2. The container for zebrafish whole embryo immunohistochemical experiment of claim 1, wherein each reagent tube comprises at least one row of well groups; the driving structure comprises at least one track, and the extending direction of the track is parallel to the arrangement direction of the hole groups; the rail is provided with an electric control sliding seat in a sliding manner, one side of the electric control sliding seat facing the hole group is provided with a lifter, and the electric control sliding seat is used for driving the lifter to slide along the rail; the lifting end of the lifter is detachably connected with the accommodating tube, and the lifter is used for driving the accommodating tube to enter and exit the reagent tube; the controller is electrically connected with the electric control sliding seat and the lifter respectively, and is used for controlling the electric control sliding seat and the lifter respectively so that the accommodating pipes sequentially enter the reagent pipes in the hole group to operate.
3. The zebrafish whole embryo immunohistochemical experiment container according to claim 2, wherein the lifting end of the lifter is provided with a mounting seat, one side of the mounting seat facing the hole group is provided with a connecting column, and one end of the connecting column away from the mounting seat is provided with an external thread; the holding pipe is provided with an internal thread at one end far away from the liquid hole, and the holding pipe is in threaded connection with the connecting column.
4. The zebrafish whole embryo immunohistochemical experiment container according to claim 3, wherein one end of the connecting column, which is far away from the mounting seat, is open, a camera is arranged in the connecting column, the camera is electrically connected with the controller, and the camera is used for shooting an operation image of the accommodating tube entering the reagent tube and sending the operation image to the controller; and a transparent water-stop sheet is arranged at the opening of the connecting column.
5. The zebrafish whole embryo immunohistochemical experiment container according to claim 1, wherein a partition plate is arranged at the bottom of the accommodating tube, the partition plate comprises a first transverse plate and a second transverse plate which are arranged in a crossed manner, the first transverse plate is perpendicular to the second transverse plate, and the first transverse plate and the second transverse plate are perpendicular to the bottom of the accommodating tube respectively.
6. The experimental container for the immunohistochemical analysis of whole zebra fish embryos of any one of claims 1 to 5, wherein a fixing plate and two supporting plates are respectively arranged in the box body, one of the supporting plates is fixedly arranged on one side of the box body, and the other supporting plate is fixedly arranged on the other side of the box body; the fixed plates are horizontally arranged in the box body, one of the supporting plates supports one end of each fixed plate, the other supporting plate supports the other end of each fixed plate, and the two fixed plates are used for enabling the fixed plates to be suspended in the box body; one side of the fixing plate facing the box cover forms an operation space in the box body, and the operation space is used for the accommodating tube to move; each reagent tube is fixed to the fixing plate.
7. The zebrafish whole embryo immunohistochemical experiment container according to claim 6, wherein one side of each supporting plate facing the box cover is provided with a limiting block; limiting through holes are formed in two ends of the fixing plate respectively, one limiting block is inserted into one limiting through hole, and the other limiting block is inserted into the other limiting through hole.
8. The zebrafish whole embryo immunohistochemical experiment container as claimed in claim 6, wherein a liquid discharge pipe is arranged at one end of the reagent pipe, which is far away from the box cover, one end of the liquid discharge pipe is communicated with the reagent pipe, and a sealing cover is arranged at the other end of the liquid discharge pipe; the sealing cover is provided with a connecting hole at one end facing the liquid discharge pipe, an internal thread is arranged in the connecting hole, an external thread is arranged at one end of the liquid discharge pipe far away from the fixed pipe, and the sealing cover is in threaded connection with the liquid discharge pipe.
9. The zebrafish whole embryo immunohistochemical experiment container according to claim 6, wherein a constant temperature space is formed in the box body at one side of the fixing plate, which is far away from the operation space, and the constant temperature space is used for storing constant temperature liquid so as to keep each reagent tube extending into the constant temperature space within a preset temperature range; a temperature detector is arranged in the constant-temperature space, the temperature detector is electrically connected with the controller, and the temperature detector is used for detecting temperature data of constant-temperature liquid and sending the temperature data to the controller; the temperature control device is arranged outside the box body, a heating pipe and a cooling pipe are respectively arranged in the constant temperature space, the heating pipe and the cooling pipe respectively penetrate through the box body to be connected with the temperature control device, and the temperature control device is used for controlling the temperature of the constant temperature liquid through the heating pipe and the cooling pipe; the controller is electrically connected with the temperature control equipment, and the controller is used for controlling the temperature control equipment according to the temperature data.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210455150.6A CN114878842A (en) | 2022-04-24 | 2022-04-24 | Whole embryo immunohistochemical experiment container of zebra fish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210455150.6A CN114878842A (en) | 2022-04-24 | 2022-04-24 | Whole embryo immunohistochemical experiment container of zebra fish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114878842A true CN114878842A (en) | 2022-08-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210455150.6A Pending CN114878842A (en) | 2022-04-24 | 2022-04-24 | Whole embryo immunohistochemical experiment container of zebra fish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114878842A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116008574A (en) * | 2022-12-14 | 2023-04-25 | 黑龙江八一农垦大学 | Immune group ization all-in-one |
-
2022
- 2022-04-24 CN CN202210455150.6A patent/CN114878842A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116008574A (en) * | 2022-12-14 | 2023-04-25 | 黑龙江八一农垦大学 | Immune group ization all-in-one |
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