CN114878440B

CN114878440B - Sample analyzer and hole blockage detection method thereof

Info

Publication number: CN114878440B
Application number: CN202210801324.XA
Authority: CN
Inventors: 池书锐; 褚聪; 甘小锋
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2022-07-08
Filing date: 2022-07-08
Publication date: 2022-10-28
Anticipated expiration: 2042-07-08
Also published as: CN114878440A

Abstract

The application discloses a sample analyzer and a hole blockage detection method thereof. The hole blockage detection method of the sample analyzer comprises the steps of establishing pressure on a pressure tank according to a pressure value required by a sample detection item; the pressure can is used for releasing pressure to drive a sample to be detected to a detection channel to execute a sample detection process; in the process of executing a sample detection process, acquiring a current pressure value in a pressure tank in real time, and establishing a pressure change curve based on the current pressure value acquired in real time; and obtaining the curve slope of the pressure change curve from the pressure change curve, and determining the hole plugging information of the detection channel based on the curve slope. Through the embodiment, the pressure change curve is established by utilizing the acquired current pressure value, and the hole plugging information of the detection channel is determined from the curve slope of the current pressure change curve, so that the hole plugging condition of the detection channel can be accurately and quickly judged, and the accuracy and the reliability of the sample detection result are improved.

Description

Sample analyzer and hole blockage detection method thereof

Technical Field

The application relates to the technical field of medical detection and analysis, in particular to a sample analyzer and a hole blockage detection method thereof.

Background

The sample analyzer comprises a blood cell analyzer, the blood cell analyzer is used as one of the sample analyzers commonly used for medical detection, and the blood cell analyzer is used for detecting parameters such as the number, the occupied proportion and the like of blood cells (red blood cells, white blood cells and blood platelets) in blood. With the progress of technology and the development of science and technology, the function of the blood cell analyzer is continuously expanded, the performance is continuously improved, the automation degree is continuously improved, and the blood cell analyzer is widely applied clinically.

The sample analyzer may include a detection channel such as a counting cell or a flow cell, the counting cell having a small pore through which the sample passes to measure the number of cells in the sample that pass through the pore. The flow chamber is provided with a detection flow channel, and cells in the sample pass through the flow channel one by one so as to realize the purpose of sample detection.

In the prior art, the situation that the micropores or the detection flow channels are blocked in the measurement process due to insufficient cleaning force of the sample analyzer or other impurities contained in the sample to be detected may occur, so that the accuracy and reliability of the detection result are affected, and therefore, the sample analyzer needs to detect the change and blocking conditions of the micropores or the detection flow channels.

Disclosure of Invention

In order to solve the problems in the prior art, the application provides a sample analyzer and a hole blockage detection method thereof.

In order to solve the above technical problem, the present application provides a method for detecting a hole blockage of a sample analyzer, the method comprising: establishing pressure for the pressure tank according to the pressure value required by the sample detection project; the pressure can is used for releasing pressure to drive a sample to be detected to a detection channel to execute a sample detection process; in the process of executing the sample detection process, acquiring a current pressure value in the pressure tank in real time, and establishing a pressure change curve based on the current pressure value acquired in real time; and obtaining the curve slope of the pressure change curve from the pressure change curve, and determining the hole plugging information of the detection channel based on the curve slope.

In one embodiment, the determining of the pore blocking information of the detection channel based on the slope of the curve includes: when the slope of the curve is determined to be kept unchanged based on the slope of the pressure change curve, the slope of the curve is compared with a preset slope to obtain a first comparison result; and determining that the detection channel is in an abnormal state of continuous hole blockage in the sample detection process when the absolute value of the curve slope is smaller than the absolute value of the preset slope and a first difference value between the absolute value of the curve slope and the absolute value of the preset slope is larger than a first preset threshold value based on the first comparison result.

In one embodiment, the obtaining a curve slope of the pressure variation curve from the pressure variation curve and determining the pore blocking information of the detection channel based on the curve slope includes: obtaining the initial slope of the initial line segment of the pressure change curve when the slope of the pressure change curve is changed based on the pressure change curve; comparing the absolute value of the initial slope with the absolute value of a preset slope, and determining that a second difference value between the absolute value of the initial slope and the absolute value of the preset slope is smaller than a second preset threshold; under the condition that the second difference value is smaller than a second preset threshold value, acquiring the characteristic slope of at least one other line segment except the initial line segment from the pressure change curve, wherein the characteristic slope is different from the initial slope; comparing the absolute value of the characteristic slope with the absolute value of the preset slope to obtain a second comparison result; determining whether the detection passage is in an abnormal state of halfway clogging of the hole in performing the sample detection based on the second comparison result.

In one embodiment, the determining whether the detection channel is in an abnormal state of halfway clogging in the process of performing the sample detection based on the second comparison result includes: determining that the detection channel is in an abnormal state of hole blocking midway in the process of executing the sample detection when the absolute value of the characteristic slope is determined to be smaller than the absolute value of the preset slope based on the second comparison result and a third difference value between the absolute value of the characteristic slope and the absolute value of the preset slope is greater than a second preset threshold; and determining that the detection channel is in a normal detection state in the process of executing the sample detection when determining that a third difference value between the absolute value of the characteristic slope and the absolute value of the preset slope is smaller than the second preset threshold value based on the second comparison result.

In one embodiment, after the determination that the detection channel is in an abnormal state of halfway clogging in the course of performing the sample detection, the method includes: acquiring the number of characteristic slopes of which the absolute value conforming to the slope is smaller than the absolute value of the preset slope and the difference value between the absolute value of the slope and the absolute value of the preset slope is larger than or equal to the second preset threshold; determining a number of times the detection channel is blocked during performance of the sample detection based on the number of characteristic slopes.

In one embodiment, after the determination that the detection channel is in an abnormal state of halfway clogging in the course of performing the sample detection, the method includes: acquiring at least one other line segment corresponding to the characteristic slope, wherein the absolute value of the slope is smaller than the absolute value of the preset slope, and the difference between the absolute value of the slope and the absolute value of the preset slope is larger than the second preset threshold; respectively acquiring the storage time of each other line segment in the pressure transformation curve; selecting the target line segment with the duration time larger than a preset time threshold from the other line segments; calculating the number of the target line segments, and determining the number of times the detection channel is blocked during the sample detection based on the number of the target line segments.

In one embodiment, the method comprises: in the process of executing the sample detection, generating a hole blocking reference picture based on parameter information obtained when the detection channel detects the sample to be detected; after determining the pore blocking information of the detection channel based on the slope of the curve, the method comprises: and when the detection channel is determined to be in the abnormal state based on the hole blocking information, performing characteristic analysis on data in the hole blocking reference image based on the hole blocking information, and further determining whether the detection channel is in the abnormal state of the hole blocking.

In an embodiment, the generating a hole plugging reference map based on parameter information obtained when the detection channel detects the sample to be detected includes: establishing a particle number graph based on the real-time particle number passing through the detection channel in the sample to be detected; the performing feature analysis on the data in the plugged hole reference map based on the plugged hole information to further determine whether the detection channel is in an abnormal state of plugged holes includes: and performing characteristic analysis on the particle number in the particle number diagram to obtain a particle number analysis result, and further determining whether the detection channel is in an abnormal state of hole blockage or not based on the hole blockage information.

In an embodiment, the generating a hole plugging reference map based on parameter information obtained when the detection channel detects the sample to be detected includes: forming a voltage profile based on voltage signals between positive and negative electrodes of the detection channel; the performing feature analysis on the data in the plugged hole reference map based on the plugged hole information to further determine whether the detection channel is in an abnormal state of plugged holes includes: and performing characteristic analysis on the voltage signals in the voltage curve graph to obtain a voltage analysis result, and further determining whether the detection channel is in the abnormal state of hole blockage based on the hole blockage information.

In an embodiment, the step of using the pressure tank to release the pressure to drive the sample to be detected to the detection channel to execute the sample detection process includes: the pressure tank is used for releasing pressure to drive a first sample to be detected to a first detection channel to execute a first sample detection process, and drive a second sample to be detected to a second detection channel to execute a second sample detection process; generating a first hole blocking reference map based on first parameter information obtained when the first detection channel detects the first sample to be detected in the process of executing the first sample detection flow, and generating a second hole blocking reference map based on second parameter information obtained when the second detection channel detects the second sample to be detected in the process of executing the second sample detection flow; after determining the plugged hole information of the detection channel based on the slope of the curve, the method comprises: when the detection channel is determined to be in an abnormal state based on the hole blocking information, judging whether a first abnormal condition exists in the first detection channel according to the first hole blocking reference image and judging whether a second abnormal condition exists in the second detection channel according to the second hole blocking reference image; determining whether the first detection channel is in the abnormal state of the blocked hole or not based on the first judgment conclusion, the second judgment conclusion and the hole blocking information, and determining whether the second detection channel is in the abnormal state of the blocked hole or not based on the first judgment conclusion, the second judgment conclusion and the hole blocking information.

In order to solve the above technical problem, the present application provides a sample analyzer, the sample analyzer includes a pressure source, a pressure tank and a detection channel, the sample analyzer is used for executing the above-mentioned hole blockage detection method, the pressure source is connected to the pressure tank, the pressure tank is connected to the detection channel, through the pressure source with the pressure tank is for providing pressure when the detection channel executes a sample detection process.

In one embodiment, the detection channel comprises a counting cell or a flow cell.

Compared with the prior art, the hole blockage detection method of the sample analyzer comprises the following steps: establishing pressure for the pressure tank according to the pressure value required by the sample detection project; the pressure can is used for releasing pressure to drive a sample to be detected to a detection channel to execute a sample detection process; in the process of executing a sample detection process, acquiring a current pressure value in a pressure tank in real time, and establishing a pressure change curve based on the current pressure value acquired in real time; and obtaining the curve slope of the pressure change curve from the pressure change curve, and determining the hole plugging information of the detection channel based on the curve slope. Through the embodiment, the pressure change curve is established by utilizing the acquired current pressure value, and the hole plugging information of the detection channel is determined from the curve slope of the current pressure change curve, so that the hole plugging condition of the detection channel can be accurately and quickly judged, and the accuracy and the reliability of the sample detection result are improved.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic structural view of a first embodiment of a sample analyzer provided herein;

FIG. 2 is a schematic structural view of a second embodiment of a sample analyzer provided herein;

FIG. 3 is a schematic flow chart diagram illustrating one embodiment of a method for detecting a plugged hole in a sample analyzer;

FIG. 4 is a schematic flowchart of a first embodiment of step S304 in FIG. 3;

fig. 5 is a flowchart illustrating a second embodiment of step S304 in fig. 3.

Detailed Description

The present application will be described in further detail with reference to the following drawings and examples. It is to be noted that the following examples are only illustrative of the present application, and do not limit the scope of the present application. Likewise, the following examples are only some examples and not all examples of the present application, and all other examples obtained by a person of ordinary skill in the art without any inventive step are within the scope of the present application.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.

Throughout the description of the present application, it is intended that the terms "mounted," "disposed," "connected," and "connected" be construed broadly and encompass, for example, fixed connections, removable connections, or integral connections unless expressly stated or limited otherwise; can be mechanically connected or electrically connected; they may be directly connected or may be connected via an intermediate medium. To one of ordinary skill in the art, the foregoing may be combined in any suitable manner with the specific meaning ascribed to the present application.

The application provides a sample analyzer, and sample analyzer is applied to blood analysis field or biochemical analysis field for detect the sample, more common sample analyzer can be blood analyzer.

The sample analyzer of the present application may be a hematology analyzer for performing one or more of routine testing of blood, testing of specific proteins, biochemical immunoassay, and testing of blood coagulation. Among them, conventional Blood tests include, but are not limited to, WBC (White Blood Cell) test, HGB (Hemoglobin) test, RBC (red Blood Cell) test, DIFF (DIFFerential, white Blood Cell five classification) test, or RET (reticulocyte) test. Specific proteins include, but are not limited to, the detection of SAA (serum amyloid A protein), CRP (C-reactive protein), TRF (tramsferrin, transferrin), hs-CRP (hypersensitive C-reactive protein), and D-Dimer (D-Dimer). Immunoassays include, but are not limited to, detection assays for PCT (procalcitonin ) and IL-6 (interleukin-6). For example, the blood analyzer is a combined-examination machine for blood routine, SAA and CRP. In other embodiments, the sample analyzer can also be used for specific protein detection and immunoassay detection, and the blood analyzer is a joint inspection integrated machine for detecting SAA, CRP, PCT and IL-6.

Referring to fig. 1 and 2, fig. 1 is a schematic structural view of a first embodiment of a sample analyzer provided in the present application, and fig. 2 is a schematic structural view of a second embodiment of the sample analyzer provided in the present application.

As shown in fig. 1, the sample analyzer includes a pressure source, a pressure canister, and a detection channel, wherein the detection channel includes a counting cell. The counting cell is filled with a conductive solvent and is divided into a front cell and a rear cell by the detection micropores of the counting cell; the front pool and the back pool are respectively provided with a positive electrode and a negative electrode, the positive electrode and the negative electrode are connected with one end of a constant current source, and the electrode arranged in the front pool, the conductive solvent, the electrode arranged in the back pool and the constant current source form a closed loop which is connected in series. The pressure sensor is provided at the pressure tank for detecting a pressure change in the pressure tank.

The pressure source can output positive pressure and negative pressure, in this embodiment, the pressure source outputs negative pressure to the pressure tank to make the pressure tank establish sufficient negative pressure, and in the process that the counting cell detects the sample, the pressure tank continuously releases negative pressure to make the particles in the counting cell pass through the detection micropore of counting cell, realize that the sample detects. The pressure sensor is used for acquiring pressure change in the pressure tank in real time, and a control system of the sample analyzer can establish a pressure change curve based on the pressure change condition of the pressure tank.

As shown in fig. 2, the sample analyzer includes a pressure source, a pressure canister, and a detection channel, wherein the detection channel includes a flow chamber. The flow chamber is provided with a cavity, a liquid inlet, a sample inlet and a liquid outlet, the cavity is used for detecting a sample, the liquid inlet is used for communicating sheath liquid, and the sheath liquid can enter the cavity from the liquid inlet. The sheath liquid enters the cavity from the liquid inlet, and forms sheath flow in the cavity of the flow chamber, and the sheath flow is in a laminar flow state, so that the flow chamber is better protected. The sample enters the flow chamber from the sample inlet of the flow chamber through the injector and can stably and uniformly pass through the sheath flow, the sheath flow has better wrapping and binding effects on the flowing sample, and the sample can be detected through the detection flow channel of the flow chamber under the wrapping of the sheath flow. The pressure sensor is provided at the pressure tank for detecting a pressure change in the pressure tank.

The pressure source can output positive pressure and negative pressure, in this embodiment, the pressure source outputs the positive pressure to the pressure tank to establish a sufficient amount of positive pressure for the pressure tank, and in the process that the flow chamber detects the sample, the pressure tank continuously releases the positive pressure to enable particles in the flow chamber to pass through the detection flow channel of the flow chamber, so as to realize sample detection. The pressure sensor is used for acquiring pressure change in the pressure tank in real time, and a control system of the sample analyzer can establish a pressure change curve based on the pressure change condition of the pressure tank.

In the embodiment of the sample analyzer, the sample is detected through the detection micropores or the detection flow channels, however, in the actual operation process, the situation that the detection micropores or the detection flow channels are blocked in the measurement process may occur due to insufficient cleaning force to the sample analyzer in advance or other impurities contained in the sample to be detected, so that the accuracy and reliability of the detection result are affected.

In order to solve the above technical problems in the prior art, the present application provides a method for detecting a hole blockage of a sample analyzer, referring to fig. 3, where fig. 3 is a schematic flow chart of an embodiment of the method for detecting a hole blockage of a sample analyzer, and specifically includes the following steps S301 to S304.

Step S301: and establishing pressure for the pressure tank according to the pressure value required by the sample detection item.

The pressure tank can temporarily store positive pressure or negative pressure, and after pressure is built on the pressure tank, the pressure can be released to the detection channel through the pressure tank, so that sample detection is realized. The pressure required for each item to be tested is different, the pressure for the pressure tank can be established based on the pressure required for the item to be tested, and illustratively, a redundancy can be added on the basis of the pressure required for the item, and the sum of the redundancy and the required pressure is used as the pressure required for the pressure tank to be established.

Step S302: and releasing pressure by using the pressure tank to drive the sample to be detected to the detection channel to execute the sample detection process.

When a sample detection process needs to be executed, the pressure in the pressure tank can be released so as to drive particles in the sample to pass through the detection micropores or the detection flow channels in the detection channel one by one, and a sample detection result is obtained from the result that the particles pass through the detection micropores.

In the case where the sample analyzer performs the sample detection using the impedance detection, the pressure tank releases the negative pressure to the detection channel to perform the sample detection through the detection channel by the negative pressure. In the case where the sample analyzer performs the sample detection using optical detection (e.g., flow detection technology), the pressure tank releases a positive pressure to the detection channel to perform the sample detection through the detection channel under the action of the positive pressure.

Step S303: and in the process of executing the sample detection process, acquiring the current pressure value in the pressure tank in real time, and establishing a pressure change curve based on the current pressure value acquired in real time.

During the execution of the sample testing process, the pressure in the pressure tank will gradually decrease with the time of the test. The pressure tank can be connected with a pressure sensor, in the process of executing a sample detection process, the pressure change condition in the pressure tank is obtained in real time through the pressure sensor, and a pressure change curve of the relation between time and pressure change is established based on the current pressure value obtained in real time.

Step S304: and obtaining the curve slope of the pressure change curve from the pressure change curve, and determining the hole plugging information of the detection channel based on the curve slope.

The sample detection may be an optical detection or an impedance detection. When the detection channel normally detects a sample to be detected, the established pressure change curve can be approximated to be in the form of a linear function, the slope of the curve is a constant value or the slope of the curve floats in an error range, wherein the horizontal axis of the coordinate axis of the pressure change curve represents pressure, and the vertical axis represents time. Specifically, when positive pressure is established in the pressure tank, the pressure in the pressure tank is gradually reduced when sample detection is performed, the pressure change curve can be approximated as a decreasing linear function, and the slope of the curve is a negative number; when negative pressure is built up in the pressure tank, the pressure in the pressure tank gradually rises when sample detection is performed, and the pressure change curve can be approximated as an increasing linear function, with the slope of the curve being a positive number.

The plugging hole information can be determined through the slope of the curve in the pressure change curve, wherein the plugging hole information can comprise: normally detecting, namely, not having the hole blocking condition; blocking holes midway, namely, not blocking holes when starting to detect, and blocking holes in the detecting process; the whole process is blocked, namely, when the sample detection is started, the situation of blocking the hole already occurs; pressure tank leaks, etc. Through the embodiment, the pressure change curve is established by utilizing the acquired current pressure value, and the hole plugging information of the detection channel is determined from the curve slope of the current pressure change curve, so that the hole plugging condition of the detection channel can be accurately and quickly judged, and the accuracy and the reliability of the sample detection result are improved.

Referring to fig. 4, fig. 4 is a schematic flowchart of a first embodiment of step S304 in fig. 3, and specifically includes the following steps S401 to S402.

Step S401: and when the slope of the curve is determined to be kept unchanged based on the slope of the curve of the pressure change curve, comparing the slope of the curve with a preset slope to obtain a first comparison result.

In this embodiment, the condition that the slope of the curve remains unchanged can be understood as a condition that the slope of the curve obtained by real-time monitoring is kept unchanged from an initial point of the pressure change curve, that is, the pressure change curve is a straight line which is approximate to a linear function, and at this time, two conditions may exist, one is a normal detection condition, and the other is a condition that the hole is blocked by the full Cheng Qingwei.

To better distinguish between the two cases, the slope of the curve may be compared with a predetermined slope, thereby obtaining the plugging information based on the first comparison result. The preset slope can be the slope of a standard curve which is similar to a linear function and is tested for multiple times, and when the slope of the curve is the preset slope, the detection channel can be determined to be in a normal working state.

Step S402: and determining that the detection channel is in an abnormal state of continuous hole blockage in the process of executing sample detection when the absolute value of the slope of the curve is smaller than the absolute value of the preset slope and the first difference value between the absolute value of the slope of the curve and the absolute value of the preset slope is larger than a first preset threshold value based on the first comparison result.

The first comparison result may include three comparison results in which the absolute value of the slope of the curve is smaller than, greater than, or equal to the absolute value of the preset slope. Determining that the absolute value of the slope of the curve is smaller than the absolute value of the preset slope, so that the probability of the situation that the detection channel is in the hole blocking state can be preliminarily judged to be high, in order to improve the accuracy of the hole blocking judgment, the absolute value of the slope of the curve and the absolute value of the preset slope can be subtracted to obtain a first difference value, when the first difference value is larger than a first preset threshold value, the difference between the slope of the curve and the preset slope is determined to be large, and the detection channel can be determined to be in the abnormal state of continuous hole blocking in the process of executing sample detection; when the first difference is smaller than or equal to a first preset threshold, the difference between the slope of the curve and the preset slope is determined to be small, and other negligible abnormal conditions which do not influence the detection result can be determined, namely the pressure in the sample detection process is determined to fluctuate within the error range, and at the moment, the detection channel can be determined to be in a normal state without blocking the hole in the sample detection process.

In the prior art, a hole blockage situation may not be identified only by a method for judging particle flow or small hole voltage, that is, when a hole blockage occurs just before sample detection, but a hole may be slightly blocked, the number of particles or voltage detected in a statistical process is in a relatively stable state, that is, the statistics of the particle flow is still stable, the hole blockage situation cannot be identified at this time, the statistics of the voltage is also stable, and the hole blockage situation cannot be identified at this time, so that the hole blockage judgment cannot be performed according to parameters of the relatively stable number of particles or voltage. In the invention, the condition that slight hole blockage occurs at the initial stage of sample detection can be identified by comparing the slope of the pressure curve detected from the pressure tank with the preset slope, and the accuracy of hole blockage judgment in the sample detection process is effectively improved by the detection method provided by the invention.

Referring to fig. 5, fig. 5 is a schematic flowchart of a second embodiment of step S304 in fig. 3, and specifically includes the following steps S501 to S505.

Step S501: and when the slope of the pressure change curve obtained based on the pressure change curve changes, acquiring the initial slope of the initial line segment of the pressure change curve.

The change in the slope of the curve means that the slope of the curve in the pressure change curve has a different slope from the initial slope. That is, the slope of the pressure variation curve is changed from the initial slope, and at this time, a situation of hole blockage may occur in the process of sample detection.

To better determine whether the above situation is present, the pressure variation curve may be analyzed, that is, an initial slope of an initial segment of the pressure variation curve is obtained, where the initial slope is an initial slope of an initial segment of the pressure variation curve when the initial segment starts from an initial point of the pressure variation curve and changes to a first slope.

Step S502: and comparing the absolute value of the initial slope with the absolute value of the preset slope, and determining that a second difference value between the absolute value of the initial slope and the absolute value of the preset slope is smaller than a second preset threshold value.

Comparing the absolute value of the initial slope with the absolute value of the preset slope can obtain three comparison results that the absolute value of the initial slope is smaller than, larger than or equal to the absolute value of the preset slope. When it is determined that a second difference between the absolute value of the initial slope and the absolute value of the preset slope is smaller than a second preset threshold, the detection channel can be considered to be in a normal working state at the beginning stage of executing sample detection, that is, it is considered that the pressure in the sample detection process fluctuates within an error range; upon determining that a second difference between the absolute value of the initial slope and the absolute value of the preset slope is greater than or equal to a second preset threshold, it may be considered that the detection channel may be an abnormal situation of a blocked hole at an initial stage of performing the sample detection.

Step S503: and acquiring the characteristic slope of at least one other line segment except the initial line segment from the pressure change curve under the condition that the second difference is smaller than a second preset threshold, wherein the characteristic slope is different from the initial slope.

And under the condition that the second difference value is smaller than a second preset threshold value, determining that the detection channel is in a normal working state at the initial stage of executing the sample detection. At this time, the characteristic slope of at least one other line segment except the initial line segment can be obtained from the pressure change curve, wherein the characteristic slope is different from the initial slope. The other line segments can be considered as the line segments that are bent in relation to the initial line segment in the pressure change curve.

Step S504: and comparing the absolute value of the characteristic slope with the absolute value of the preset slope to obtain a second comparison result.

The second comparison result may include three second comparison results in which the absolute value of the characteristic slope is smaller than, greater than, or equal to the absolute value of the preset slope.

Step S505: and determining whether the detection passage is in an abnormal state of halfway hole blocking in the process of performing the sample detection based on the second comparison result.

The second comparison result may include that when the absolute value of the characteristic slope is smaller than the absolute value of the preset slope, it is determined that the pressure in the sample detection process fluctuates within the error range, that is, it may be determined that the detection channel is in a normal state without blocking the hole in the process of performing the sample detection; the second comparison result may include that when the absolute value of the characteristic slope is greater than or equal to the absolute value of the preset slope, it may be determined that the probability that the detection channel is in an abnormal state of a midway hole blocking in the process of performing the sample detection is high.

In one embodiment, the step of determining whether the detection channel is in an abnormal state of halfway clogging of the hole in performing the sample detection based on the second comparison result (step S505) includes: and determining that the detection channel is in an abnormal state of midway hole plugging in the process of executing sample detection when the absolute value of the characteristic slope is smaller than the absolute value of the preset slope and a third difference value between the absolute value of the characteristic slope and the absolute value of the preset slope is larger than a second preset threshold value based on the second comparison result. And when determining that a third difference value between the absolute value of the characteristic slope and the absolute value of the preset slope is smaller than a second preset threshold value based on the second comparison result, determining that the detection channel is in a normal detection state in the process of executing sample detection.

The second comparison result may include three comparison results in which the absolute value of the characteristic slope is smaller than, greater than, or equal to the absolute value of the preset slope. When the absolute value of the characteristic slope is determined to be smaller than the absolute value of the preset slope, the condition that the detection channel is possibly in the midway hole blocking state can be preliminarily judged, the absolute value of the characteristic slope and the absolute value of the preset slope can be subjected to difference to obtain a third difference value, when the third difference value is larger than a second preset threshold value, the difference between the characteristic slope and the preset slope is determined to be large, and the detection channel can be determined to be in the midway hole blocking abnormal state in the sample detection process; when the third difference is smaller than or equal to a second preset threshold, determining that the difference between the characteristic slope and the preset slope is smaller, and determining that the detection channel is in a normal detection state without blocking the hole in the process of performing sample detection.

In one embodiment, after determining that the detection channel is in an abnormal state of halfway clogging in the sample detection, the method includes: acquiring the number of characteristic slopes of which the absolute value conforming to the slope is smaller than the absolute value of the preset slope and the difference value between the absolute value of the slope and the absolute value of the preset slope is larger than or equal to a second preset threshold; determining the number of times the detection channel is blocked during the process of performing the sample detection based on the number of characteristic slopes.

After the abnormal state of the blocked hole is determined, the number of times of detecting the blocked hole of the channel in the process of executing sample detection can be determined through the number of characteristic slopes in the pressure change curve, so that the situation of the blocked hole of the channel can be accurately and quickly determined based on the number of times of detecting the blocked hole of the channel, and the accuracy and the reliability of a sample detection result can be improved.

In another embodiment, after determining that the detection channel is in an abnormal state of halfway clogging in performing the sample detection, the method includes: acquiring at least one other line segment corresponding to the characteristic slope, wherein the absolute value of the slope is smaller than the absolute value of the preset slope, and the difference between the absolute value of the slope and the absolute value of the preset slope is larger than a second preset threshold, and respectively acquiring the storage time of each other line segment in the pressure conversion curve; selecting a target line segment with the access time longer than a preset time threshold from other line segments; the number of target line segments is calculated, and the number of times the detection channel is blocked during the process of performing the sample detection is determined based on the number of target line segments.

The absolute value of the slope is smaller than the absolute value of the preset slope, and the characteristic slope of which the difference value between the absolute value of the slope and the absolute value of the preset slope is larger than a second preset threshold value is the characteristic slope of a line segment which is bent with the initial line segment in the pressure change curve. The pressure change curve is a relation graph of pressure in the pressure tank and time, after at least one other line segment corresponding to the characteristic slope is obtained, the duration time of the line segment in the pressure change curve can be determined, when the duration time is short, namely the duration time is smaller than a preset time threshold value, the situation that a pipeline in a short hole blocking situation or a pipeline in a liquid path is blocked temporarily can be considered in the detection process, the situation is a reasonable situation which can be accepted in the sample detection process and does not influence the result, and the situation that the duration time is short can not be considered in the calculation of the hole blocking times; when the duration is longer, that is, the duration is greater than the preset time threshold, it may be considered that the hole blocking condition is a certain period of time in the detection process, which is an abnormal condition that may affect the detection result in the sample detection process, and the situation that the duration is greater than the preset time threshold needs to be considered in calculating the hole blocking times. Selecting a target line segment with the access time longer than a preset time threshold from other line segments; the accuracy of the times of hole blockage detection can be improved by only calculating the number of the target line segments as the times of detecting the hole blockage of the channel in the process of executing sample detection.

In other embodiments, in order to further improve the accuracy and reliability of the sample analyzer, other parameters may be referred to and combined with the pressure change curve to jointly serve as information for judging whether the detection channel is blocked.

Specifically, the method further comprises the steps of generating a hole plugging reference map based on parameter information obtained when the detection channel detects the sample to be detected in the process of executing sample detection; after determining the plugged hole information of the detection channel based on the slope of the curve (step S304), the method includes: and when the detection channel is determined to be in the abnormal state based on the hole plugging information, performing characteristic analysis on data in the hole plugging reference map based on the hole plugging information, and further determining whether the detection channel is in the abnormal state of the hole plugging.

The detection channel can be a flow chamber or a counting cell, and when the detection channel is the flow chamber, the parameter information can be a particle number map; when the detection channel is a counting cell, the parameter information may be a voltage graph formed by a particle number map and/or a voltage signal. When determining that the detection channel is in the abnormal state based on the hole plugging information, in order to further determine whether the detection channel is plugged, feature analysis may be performed on data in the hole plugging reference map based on the hole plugging information, so as to further determine whether the detection channel is in the abnormal state of the hole plugging.

In an embodiment, generating a hole plugging reference map based on parameter information obtained when a detection channel detects a sample to be detected includes: establishing a particle number graph based on the real-time particle number passing through a detection channel in a sample to be detected; carrying out characteristic analysis on the data in the plugged hole reference diagram based on the plugged hole information, and further determining whether the detection channel is in an abnormal state of the plugged hole, wherein the characteristic analysis comprises the following steps: and performing characteristic analysis on the particle number in the particle number graph to obtain a particle number analysis result, and further determining whether the detection channel is in an abnormal state of hole blockage based on the hole blockage information.

In the process of detecting the sample in the flow chamber or the counting cell, the particles in the sample can pass through the detection flow channel or the detection micropores of the detection channel one by one, and then a particle number and time relation graph, namely a particle number graph, is established based on the particle number passing through the detection micropores. In the case that the hole blocking information is abnormal, the particle number in the particle number map may be subjected to feature analysis to obtain a particle number analysis result, so as to further determine whether the detection channel is in an abnormal state of hole blocking based on the analysis result. In the particle number diagram, if a curve of the particle number diagram changes suddenly in a certain period of time and the slope of the curve of the pressure change curve diagram changes in the certain period of time, it can be considered that no particle passes through the detection flow channel in the certain period of time, and it is determined that the detection channel is in an abnormal state of hole blocking; in the particle number diagram, the curve of the whole detection time period does not have mutation, namely the detection flow channel of the whole detection time period is considered to have particles passing through, and the detection channel is determined to be in a normal detection state.

In another embodiment, generating a hole plugging reference map based on parameter information obtained when a detection channel detects a sample to be detected includes: forming a voltage curve graph based on voltage signals between a positive electrode and a negative electrode of the detection channel; carrying out characteristic analysis on the data in the plugged hole reference diagram based on the plugged hole information, and further determining whether the detection channel is in an abnormal state of the plugged hole, wherein the characteristic analysis comprises the following steps: and performing characteristic analysis on the voltage signals in the voltage curve graph to obtain a voltage analysis result, and further determining whether the detection channel is in the abnormal state of hole plugging or not based on the hole plugging information.

When the detection channel comprises a counting cell, the counting cell is filled with a conductive solvent and is divided into a front cell and a rear cell by a detection micropore of the counting cell; the front pool and the back pool are respectively provided with a positive electrode and a negative electrode, the positive electrode and the negative electrode are connected with one end of a constant current source, and the electrode arranged in the front pool, the conductive solvent, the electrode arranged in the back pool and the constant current source form a closed loop which is connected in series. According to the technical principle, a voltage curve graph can be formed based on voltage signals between the positive electrode and the negative electrode of the detection channel, characteristic analysis is carried out on the voltage signals in the voltage curve graph to obtain a voltage analysis result, and whether the detection channel is in the abnormal state of hole blockage is further determined based on hole blockage information. For example, when the voltage value in the voltage graph remains unchanged, it may be determined that the detection channel is in a normal detection state; when the voltage value in the voltage curve graph changes suddenly in a certain time period and the slope of the curve of the voltage curve graph changes in the certain time period, the detection channel can be determined to be in the abnormal state of hole blocking.

Through the embodiment, whether the detection channel is blocked or not is further judged by utilizing the combination of the pressure change curve and other reference parameters, and the accuracy of judging whether the detection channel is blocked or not can be improved.

In the above embodiment, the pressure tank corresponds to one detection channel, in other embodiments, the pressure tank may also provide pressure for a plurality of detection channels at the same time, and when the pressure tank may also provide pressure for a plurality of detection channels at the same time, it may be determined whether a pressure releasing process of the pressure tank is abnormal through a pressure change curve, and then the abnormal detection channel is searched for from other parameters.

Specifically, taking the pressure tank corresponding to the two detection channels as an example, in this embodiment, the step of using the pressure released by the pressure tank to drive the sample to be detected to the detection channel to execute the sample detection process (step S302) includes: the pressure tank is used for releasing pressure to drive a first sample to be detected to a first detection channel to execute a first sample detection process, and drive a second sample to be detected to a second detection channel to execute a second sample detection process; in the process of executing the first sample detection process, a first hole blocking reference map is generated based on first parameter information obtained when the first detection channel detects the first sample to be detected, and in the process of executing the second sample detection process, a second hole blocking reference map is generated based on second parameter information obtained when the second detection channel detects the second sample to be detected.

When a sample detection process needs to be executed, the pressure in the pressure tank can be released so as to simultaneously drive particles of samples in the two detection channels to pass through the detection micropores or the detection flow channels in the detection channels one by one, and a sample detection result is obtained from the result that the particles pass through the detection micropores or the detection flow channels. The first detection channel and the second detection channel can be a counting cell or a flow chamber, when the detection channel is the counting cell, the reference information can comprise a particle number and/or a voltage signal, and the pore-blocking reference map comprises a voltage curve graph and/or a particle number map; when the detection channel is a flow cell, the reference information may include a particle count, and the plugged hole reference map includes a particle count map.

After determining the plugged hole information of the detection channel based on the slope of the curve (step S304), the method includes: when the detection channel is determined to be in an abnormal state based on the hole plugging information, judging whether a first abnormal condition exists in the first detection channel according to the first hole plugging reference image and judging whether a second abnormal condition exists in the second detection channel according to the second hole plugging reference image; determining whether the first detection channel is in the abnormal state of the blocked hole or not based on the first judgment conclusion, the second judgment conclusion and the hole blocking information, and determining whether the second detection channel is in the abnormal state of the blocked hole or not based on the first judgment conclusion, the second judgment conclusion and the hole blocking information.

When it is determined that the detection channel is in an abnormal state based on the hole plugging information, in order to further determine that the first detection channel and/or the second detection channel is plugged, it may be determined whether the first detection channel has a first abnormal condition and whether the second detection channel has a second abnormal condition, simultaneously, by the first hole plugging reference map and the second hole plugging reference map. The abnormal condition includes an abnormal condition that the detection channel is blocked, for example, in the case that the slope of the curve in the pressure change diagram changes, when the reference diagram is a particle number diagram, if the curve of the particle number diagram changes suddenly in a certain period of time in the first detection channel and/or the second detection channel and the slope of the curve of the pressure change curve graph changes in the period of time, it can be considered that the detection channel in which the curve of the particle number diagram changes suddenly in the period of time does not pass through particles, and then it is determined that the corresponding detection channel is in the abnormal condition of blocking the hole; if the change of the slope of the curve in the pressure change diagram floats in the error range and the curve of the whole time period does not change suddenly in the particle number diagram, namely the particles pass through the detection micropores of the whole time period, the detection channel is determined to be in a normal detection state; when the reference graph is a voltage change graph, when the voltage value in the voltage curve graph is kept unchanged, the detection channel can be determined to be in a normal detection state; under the condition that the slope of the curve in the pressure change diagram changes, if the voltage value of the first detection channel and/or the second detection channel in a certain period of time changes and the slope of the curve of the pressure change diagram changes in the period of time, it can be considered that no particle passes through the detection channel with the sudden change of the curve of the voltage change diagram in the period of time, and further the corresponding detection channel can be determined to be in an abnormal condition.

Whether a first abnormal condition exists in the first detection channel or not can be obtained according to the hole plugging reference image of the first detection channel, whether a second abnormal condition exists in the second detection channel or not can be obtained according to the hole plugging reference image of the second detection channel, namely whether the first detection channel is in the abnormal state of the plugged hole or not can be determined based on the first judgment conclusion, the second judgment conclusion and the hole plugging information, and whether the second detection channel is in the abnormal state of the plugged hole or not can be determined based on the first judgment conclusion, the second judgment conclusion and the hole plugging information.

In the above embodiment, the pressure tank can also provide pressure for the two detection channels at the same time, determine whether the pressure releasing process of the pressure tank is abnormal or not through the pressure change curve, and then determine the abnormal detection channel through the curve slope of the pressure change curve graph and other parameters, so that the detection channel with the abnormal hole plugging can be quickly and accurately found, and the accuracy of sample detection and the timeliness of sample detection can be conveniently improved.

In addition, if the above functions are implemented in the form of software functions and sold or used as a standalone product, the functions may be stored in a storage medium readable by a mobile terminal, that is, the present application also provides a storage device storing program data, which can be executed to implement the method of the above embodiments, the storage device may be, for example, a usb disk, an optical disk, a server, etc. That is, the present application may be embodied as a software product, which includes several instructions for causing an intelligent terminal to perform all or part of the steps of the methods described in the embodiments.

In the description of the present application, reference to the description of "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like is intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present application, "plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

Any process or method descriptions in flow charts or otherwise described herein may be understood as representing modules, segments, or portions of code which include one or more executable instructions for implementing specific logical functions or steps of the process, and the scope of the preferred embodiments of the present application includes other implementations in which functions may be executed out of order from that shown or discussed, including substantially concurrently or in reverse order, depending on the functionality involved, as would be understood by those reasonably skilled in the art of the present application.

The logic and/or steps represented in the flowcharts or otherwise described herein, such as an ordered listing of executable instructions that can be viewed as implementing logical functions, can be embodied in any computer-readable medium for use by or in connection with an instruction execution system, apparatus, or device (e.g., a personal computer, server, network device, or other system that can fetch the instructions from the instruction execution system, apparatus, or device and execute the instructions). For the purposes of this description, a "computer-readable medium" can be any means that can contain, store, communicate, propagate, or transport the program for use by or in connection with the instruction execution system, apparatus, or device. More specific examples (a non-exhaustive list) of the computer-readable medium would include the following: an electrical connection (electronic device) having one or more wires, a portable computer diskette (magnetic device), a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable medium could even be paper or another suitable medium upon which the program is printed, as the program can be electronically captured, via for instance optical scanning of the paper or other medium, then compiled, interpreted or otherwise processed in a suitable manner if necessary, and then stored in a computer memory.

The above description is only an embodiment of the present application, and is not intended to limit the scope of the present application, and all equivalent structures or equivalent processes performed by the present application and the contents of the attached drawings, which are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.

Claims

1. A method of detecting a plugged hole in a sample analyzer, the method comprising:

establishing pressure on the pressure tank according to the pressure value required by the sample detection item;

the pressure can is used for releasing pressure to drive a sample to be detected to a detection channel to execute a sample detection process;

in the process of executing the sample detection process, acquiring a current pressure value in the pressure tank in real time, and establishing a pressure change curve based on the current pressure value acquired in real time;

obtaining the curve slope of the pressure change curve from the pressure change curve, and determining the hole plugging information of the detection channel based on the curve slope;

in the process of executing the sample detection, generating a hole blocking reference map based on parameter information obtained when the detection channel detects the sample to be detected, wherein the hole blocking reference map comprises a particle number map and/or a voltage curve map, the particle number map is established based on the real-time particle number of the sample to be detected passing through the detection channel, and the voltage curve map is formed based on a voltage signal between a positive electrode and a negative electrode of the detection channel;

performing characteristic analysis on the particle number in the particle number diagram to obtain a particle number analysis result, and further determining whether the detection channel is in an abnormal state of hole blockage based on the hole blockage information; and/or the presence of a gas in the gas,

and performing characteristic analysis on the voltage signals in the voltage curve graph to obtain a voltage analysis result, and further determining whether the detection channel is in the abnormal state of hole blockage based on the hole blockage information.

2. The method of claim 1, wherein the determining the hole plugging information of the detection channel based on the slope of the curve comprises:

when the slope of the curve is determined to be kept unchanged based on the slope of the pressure change curve, the slope of the curve is compared with a preset slope to obtain a first comparison result;

and determining that the detection channel is in an abnormal state of continuous hole blockage in the sample detection process when the absolute value of the curve slope is smaller than the absolute value of the preset slope and a first difference value between the absolute value of the curve slope and the absolute value of the preset slope is larger than a first preset threshold value based on the first comparison result.

3. The method according to claim 1, wherein obtaining a slope of the pressure variation curve from the pressure variation curve and determining the hole plugging information of the detection channel based on the slope of the curve comprises:

obtaining the initial slope of the initial line segment of the pressure change curve when the slope of the pressure change curve is changed based on the pressure change curve;

comparing the absolute value of the initial slope with the absolute value of a preset slope, and determining that a second difference value between the absolute value of the initial slope and the absolute value of the preset slope is smaller than a second preset threshold value;

under the condition that the second difference value is smaller than a second preset threshold value, acquiring the characteristic slope of at least one other line segment except the initial line segment from the pressure change curve, wherein the characteristic slope is different from the initial slope;

comparing the absolute value of the characteristic slope with the absolute value of the preset slope to obtain a second comparison result;

determining whether the detection passage is in an abnormal state of halfway clogging of the hole in performing the sample detection based on the second comparison result.

4. The hole plugging detection method according to claim 3, wherein said determining whether the detection passage is in an abnormal state of halfway plugging in the process of performing the sample detection based on the second comparison result comprises:

determining that the detection channel is in an abnormal state of hole blocking midway in the process of executing the sample detection when the absolute value of the characteristic slope is determined to be smaller than the absolute value of the preset slope based on the second comparison result and a third difference value between the absolute value of the characteristic slope and the absolute value of the preset slope is greater than a second preset threshold;

and determining that the detection channel is in a normal detection state in the process of executing the sample detection when determining that a third difference value between the absolute value of the characteristic slope and the absolute value of the preset slope is smaller than the second preset threshold value based on the second comparison result.

5. The plugged hole detection method according to claim 4, wherein after the determination that the detection channel is in an abnormal state of a halfway plugged hole in the course of performing the sample detection, the method comprises:

acquiring the number of characteristic slopes of which the absolute value conforming to the slope is smaller than the absolute value of the preset slope and the difference value between the absolute value of the slope and the absolute value of the preset slope is larger than or equal to the second preset threshold;

determining a number of times the detection channel is plugged in the course of performing the sample detection based on the number of characteristic slopes.

6. The plugged hole detection method according to claim 4, wherein after the determination that the detection channel is in an abnormal state of a halfway plugged hole in the course of performing the sample detection, the method comprises:

acquiring at least one other line segment corresponding to the characteristic slope, wherein the absolute value of the slope is smaller than the absolute value of the preset slope, and the difference between the absolute value of the slope and the absolute value of the preset slope is larger than the second preset threshold;

respectively acquiring the storage duration of each other line segment in the pressure change curve;

selecting the target line segment with the duration time larger than a preset time threshold from the other line segments;

calculating the number of the target line segments, and determining the number of times the detection channel is blocked during the sample detection based on the number of the target line segments.

7. The method according to claim 1, wherein the step of using the pressure released by the pressure tank to drive the sample to be detected to the detection channel to perform a sample detection process comprises:

the pressure can is used for releasing pressure to drive a first sample to be detected to a first detection channel to execute a first sample detection process, and drive a second sample to be detected to a second detection channel to execute a second sample detection process;

generating a first hole blocking reference picture based on first parameter information obtained when the first detection channel detects the first sample to be detected in the process of executing the first sample detection flow, and generating a second hole blocking reference picture based on second parameter information obtained when the second detection channel detects the second sample to be detected in the process of executing the second sample detection flow; wherein the first plugged hole reference map comprises a particle count map and/or a voltage profile, and the second plugged hole reference map comprises a particle count map and/or a voltage profile;

after determining the plugged hole information of the detection channel based on the slope of the curve, the method comprises:

when the detection channel is determined to be in an abnormal state based on the hole blocking information, judging whether a first abnormal condition exists in the first detection channel according to the first hole blocking reference image and judging whether a second abnormal condition exists in the second detection channel according to the second hole blocking reference image;

determining whether the first detection channel is in the abnormal state of the blocked hole or not based on the first judgment conclusion, the second judgment conclusion and the hole blocking information, and determining whether the second detection channel is in the abnormal state of the blocked hole or not based on the first judgment conclusion, the second judgment conclusion and the hole blocking information.

8. A sample analyzer, comprising a pressure source, a pressure tank and a detection channel, wherein the sample analyzer is used for performing the method according to any one of claims 1 to 7, the pressure source is connected to the pressure tank, the pressure tank is connected to the detection channel, and the pressure source and the pressure tank provide pressure for the detection channel during a sample detection process.

9. The sample analyzer of claim 8, wherein the detection channel comprises a counting cell or a flow cell.