CN114870067A

CN114870067A - Elastin hydrogel material for wound repair and preparation method thereof

Info

Publication number: CN114870067A
Application number: CN202210498777.XA
Authority: CN
Inventors: 严乐平; 徐云升; 罗亮; 陈佳礽; 田多梅; 万欢欢
Original assignee: Seventh Affiliated Hospital Of Sun Yat Sen University Shenzhen
Current assignee: Seventh Affiliated Hospital Of Sun Yat Sen University Shenzhen
Priority date: 2022-05-09
Filing date: 2022-05-09
Publication date: 2022-08-09
Anticipated expiration: 2042-05-09
Also published as: CN114870067B

Abstract

The invention discloses an elastin hydrogel material for wound repair and a preparation method thereof, which is to dissolve soluble elastin in deionized water or buffer solution to prepare elastin aqueous solution; then adding acrylate-polyethylene glycol-N-hydroxysuccinimide or methacrylate-polyethylene glycol-N-hydroxysuccinimide, and reacting under the conditions of filling nitrogen at normal temperature and keeping out of the sun to obtain modified elastin; dialyzing the modified elastin in deionized water, freezing and freeze-drying to obtain dry modified elastin; and dissolving the dry modified elastin in deionized water or phosphate physiological buffer solution, adding a photoinitiator, and irradiating under a light source with a certain wavelength to prepare the elastin hydrogel material. The invention has simple process, quick reaction, high grafting rate and high elastin content.

Description

Elastin hydrogel material for wound repair and preparation method thereof

Technical Field

The invention belongs to the field of biological dressings, and particularly relates to an elastin hydrogel material for wound repair and a preparation method thereof.

Background

A great number of full-thickness skin defects caused by various accidents every year are urgently needed to be repaired for patients with wound surface. At present, the clinical treatment method of wound defects mainly comprises autologous or allogeneic skin transplantation. However, autograft donors have limited donor sites and cause damage to donor sites, and xenotransplantation is prone to immunological rejection and safety problems. The development of biological materials and regenerative medicine in recent years provides new hope for wound repair. The biological material can be used as wound dressing or dermal repair filler for promoting wound healing. The hydrogel material can absorb exudates to create a moist environment for the wound, and can form a physical barrier between the wound surface and the outside to isolate bacterial invasion, so that great application potential is shown in wound surface repair.

Elastin (Elastin) is a water-insoluble three-dimensional network structure formed by cross-linking soluble tropoelastin secreted by cells under the action of in vivo enzymes. Elastin is present in tissues and organs such as skin, lung, blood vessel, etc., and plays a role in maintaining the elasticity of the tissues and organs. The elastin has the functions of promoting skin cell migration and proliferation and promoting vascularization, so the elastin-based biomaterial is an ideal wound repair material. Currently, there are two main categories of elastin-based biomaterials: the first type is natural elastin extracted from animals, which has large yield and low cost, is suitable for large-scale production and application, but is insoluble in water and difficult to process, and is generally applied after being hydrolyzed into soluble elastin; the second type is the synthesis of human tropoelastin or elastin-like polypeptide by bacteria through genetic engineering methods, but the method has low yield and high cost. Therefore, if the hydrolyzed soluble natural elastin is modified to prepare the hydrogel material capable of being formed in situ, the requirements of wound repair of different depths and shapes can be met, and the application of the hydrogel material in wound repair is greatly expanded.

The literature reports the research of preparing hydrogel by compounding modified natural elastin with polyethylene glycol diacrylate and gelatin (Polymers,2020,12,670), which comprises the following specific steps: (1) respectively reacting soluble elastin and gelatin with annular 2-iminothiolane hydrochloride (Traut's reagent), grafting sulfydryl on the elastin and the gelatin, wherein the reaction takes about 1 hour, and the grafted sulfydryl is easy to generate intramolecular cross-linking, thereby influencing the subsequent reaction efficiency; (2) respectively reacting the elastin grafted with the sulfydryl and the gelatin grafted with the sulfydryl with polyethylene glycol diacrylate (the molecular weight is 8 kDa; two double bonds are respectively arranged at two ends of a molecular chain) for overnight to respectively obtain an elastin-polyethylene glycol (the double bonds can further carry out crosslinking reaction) hydrogel precursor and a gelatin-polyethylene glycol precursor, wherein the weight ratio of the elastin or the gelatin in the precursor is about 60%; (3) mixing the gelatin-polyethylene glycol precursor and the elastin-polyethylene glycol precursor, and crosslinking under the action of ultraviolet light to prepare the elastin/gelatin/polyethylene glycol hydrogel. Therefore, the hydrogel prepared in the document has many (two) earlier reaction steps, long time consumption (the first reaction step is 1 hour, and the second reaction step needs to be over night), low efficiency (the mercapto group is easy to generate intramolecular cross-linking to influence the subsequent grafting reaction, and the mercapto group can simultaneously react with double bonds at two ends of the polyethylene glycol diacrylate to cause the polyethylene glycol diacrylate grafted to the elastin to lose the photocrosslinking capacity); the polyethylene glycol diacrylate ester used in the reaction has large molecular weight, so that the protein content in the final product is only about 60 percent, and the cell adhesion of the hydrogel can be weakened due to the high polyethylene glycol content; in addition, the grafting yield in this reaction was only about 60% (the change in the primary amine group content before and after modification was measured).

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides an elastin hydrogel material for wound repair, which is simple in process, rapid in reaction, high in grafting rate and elastin content, and a preparation method thereof.

The purpose of the invention is realized by the following technical scheme:

a preparation method of an elastin hydrogel material for wound repair comprises the following steps:

(1) dissolving 0.5-2 parts by mass of soluble elastin in 100 parts of deionized water or buffer solution to prepare an elastin aqueous solution;

(2) adding 0.1-1 part by mass of acrylate-polyethylene glycol-N-hydroxysuccinimide (AC-PEG-NHS) or methacrylate-polyethylene glycol-N-hydroxysuccinimide (MeAC-PEG-NHS) into the elastin aqueous solution, and filling nitrogen at normal temperature and reacting under the conditions of light resistance to obtain modified elastin;

(3) dialyzing the modified elastin in deionized water, placing the dialyzed modified elastin aqueous solution in an environment with the temperature of < -15 ℃ to freeze, and then freeze-drying in a freeze dryer to obtain dry modified elastin;

(4) dissolving 0.5-20 parts by mass of the dry modified elastin in 100 parts of deionized water or phosphate Physiological (PBS) buffer solution, adding a photoinitiator, and then irradiating under a light source with a certain wavelength to prepare the elastin hydrogel material.

Preferably, in step (1), the soluble elastin is derived from a hydrolysate of insoluble elastin in fish or mammalian tissue.

Preferably, in the step (1), the buffer solution is 0.01M phosphate physiological buffer solution or 0.01-0.06M NaH ₂ PO ₄ A buffer solution; the pH value of the buffer solution is 8.0-8.3.

Preferably, in the step (2), the polymerization degree of PEG in the AC-PEG-NHS or the MeAC-PEG-NHS is 4-20.

Preferably, in the step (2), the reaction time of the AC-PEG-NHS or MeAC-PEG-NHS and the elastin aqueous solution is 0.5-3 hours.

Preferably, in the step (3), the dialysis time of the modified elastin is 24-48 hours, and the cut-off molecular weight of the dialysis bag is 1-50 kDa.

Preferably, in step (4), the phosphate physiological buffer solution has a concentration of 0.01M and a pH value of 7.0-7.5.

Preferably, in the step (4), the wavelength of the irradiation light is 365nm or 405 nm; when 365nm ultraviolet light is adopted for irradiation, the corresponding photoinitiator is an ultraviolet photoinitiator, such as 2-hydroxy-4' - (2-hydroxyethoxy) -2-methyl propiophenone (Irgacure 2959); when irradiated with 405nm blue light, the corresponding photoinitiator is a blue light initiator, such as lithium phenyl-2, 4, 6-trimethylbenzoylphosphinate (LAP). The final concentration of the added initiator is 0.01-0.5%.

Preferably, in the step (4), the photocrosslinking irradiation time is 0.5-10 minutes.

In the step (4), 0.1-10 parts by mass of other water-soluble polymers with photo-crosslinking functional groups can be added, the water-soluble polymers and 0.5-10 parts by mass of dry modified elastin are dissolved in 100 parts of deionized water or phosphate physiological buffer solution, 0.01-0.5 part of photoinitiator is added, and then the elastin composite hydrogel material is formed by irradiating for a certain time under a light source with a certain wavelength.

The other water-soluble polymers having a photo-crosslinking functional group include, but are not limited to, the original or modified products of the following polymers: protein polymers (gelatin, collagen, silk fibroin, polypeptide, keratin), polysaccharide polymers (alginic acid, hyaluronic acid, chitosan, gellan gum, carrageenan), other synthetic water-soluble polymers, or any combination thereof.

An elastin hydrogel material for wound repair is prepared by the method.

The principle of the invention is as follows: acrylate-polyethylene glycol-N-hydroxysuccinimide (AC-PEG-NHS) or methacrylate-polyethylene glycol-N-hydroxysuccinimide (MeAC-PEG-NHS) is adopted to react with elastin, and double bonds which can generate photo-crosslinking reaction are grafted to elastin. In the reaction of AC-PEG-NHS or MeAC-PEG-NHS adopted by the invention, the hydroxysuccinimide ester group can directly generate nucleophilic reaction with primary amine on elastin, thereby grafting double bond to the primary amine group of elastin. The modified elastin prepared by the invention contains double bonds, and can mutually generate addition reaction under the irradiation of a photocatalyst and a light source with a certain wavelength to form three-dimensional cross-linked polymer network-hydrogel.

Compared with the prior art, the invention has the following advantages and effects:

(1) the steps for preparing the photocrosslinking elastin hydrogel precursor, namely the modified elastin, only need one step, while the method in the prior art needs two steps, so that the method is simpler.

(2) The modification reaction time of the elastin grafting double bond can be controlled within 0.5-3 hours, while the reaction of the elastin grafting double bond (the reaction with the polyethylene glycol diacrylate) in the prior art needs to be over night, the reaction time is longer, and the preparation efficiency of the method is high.

(3) The grafting efficiency of the primary amino group on the elastin can reach more than 90%, while the grafting efficiency of the prior art to the primary amino group on the elastin is only about 60%, and the method of the invention has high modification grafting efficiency.

(4) The polymerization degree of PEG in the graft acrylate-polyethylene glycol-N-hydroxysuccinimide (AC-PEG-NHS) or methacrylate-polyethylene glycol-N-hydroxysuccinimide (MeAC-PEG-NHS) can be controlled to be 1-20, and the molecular weight can be controlled to be 300-1300 Da; the molecular weight of the grafted polyethylene glycol diacrylate ester in the prior art is 8000; therefore, the protein content of the modified final product of the invention can reach more than 90 percent at most, while the protein content of the modified final product of the prior art is about 60 percent at most; since polyethylene glycol has anti-adhesion properties, the increased protein content of the hydrogel of the invention contributes to the increased cell adhesion of the hydrogel of the invention.

(5) The elastin modification product prepared in the invention can be independently prepared into photo-crosslinking hydrogel, while the prior art only prepares elastin/gelatin/polyethylene glycol composite hydrogel and does not prepare pure modified elastin hydrogel; therefore, the method of the invention has more advantages in preparing the elastin hydrogel and can also verify the effect of the pure elastin hydrogel in wound repair.

Drawings

FIG. 1 is a schematic reaction diagram of AC-PEG-NHS modified elastin.

FIG. 2 is a Fourier transform Infrared Spectroscopy (FTIR) of unmodified Elastin (Elastin) and modified Elastin (Elastin-PEG-AC) prepared in example 9.

Fig. 3 is an appearance view of the elastin hydrogel prepared in example 10.

Detailed Description

In order that the invention may be readily understood, reference will now be made in detail to the specific embodiments of the invention. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that, for a person skilled in the art, many variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Example 1

This example provides a method for modifying photocrosslinkable elastin, comprising the following steps: 0.05g of soluble elastin was dissolved in 10mL of 0.01M phosphate physiological buffer pH 8.0 to prepare an aqueous elastin solution. To the elastin aqueous solution was added 0.01g methacrylate-polyethylene glycol-N-hydroxysuccinimide (polyethylene glycol degree of polymerization 4), and the reaction was stirred at room temperature under nitrogen and in the dark for 3 hours. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin.

Example 2

This example provides a method for modifying photocrosslinkable elastin, comprising the following steps: 0.2g of soluble elastin was dissolved in 10mL of 0.01M phosphate physiological buffer pH 8.0 to prepare an aqueous elastin solution. To the elastin aqueous solution was added 0.1g methacrylate-polyethylene glycol-N-hydroxysuccinimide (polyethylene glycol polymerization degree 20), and the reaction was stirred at room temperature under nitrogen and in the dark for 0.5 hours. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin.

Example 3

This example provides a method for modifying photocrosslinkable elastin, comprising the following steps: 0.15g of soluble elastin was dissolved in 10mL of 0.01M phosphate physiological buffer pH 8.3 to prepare an aqueous elastin solution. To the elastin aqueous solution was added 0.08g methacrylate-polyethylene glycol-N-hydroxysuccinimide (polyethylene glycol polymerization degree 10), and the reaction was stirred at room temperature under nitrogen and in the dark for 2 hours. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin.

Example 4

This example provides a method for modifying photocrosslinkable elastin, comprising the following steps: 0.05g of soluble elastin dissolved in 10mL of 0.01M NaH at pH 8.0 ₂ PO ₄ The buffer solution is prepared into an elastin water solution. To the elastin aqueous solution was added 0.01g methacrylate-polyethylene glycol-N-hydroxysuccinimide (polyethylene glycol polymerization degree 8), and the reaction was stirred at room temperature under nitrogen and in the dark for 1 hour. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin.

Example 5

This example provides a method for modifying photocrosslinkable elastin, comprising the steps of: 0.15g of soluble elastin dissolved in 10mL of 0.01M NaH at pH 8.3 ₂ PO ₄ The buffer solution is prepared into an elastin water solution. 0.05g of methacrylate-polyethylene glycol-N-hydroxysuccinimide (the polymerization degree of polyethylene glycol is 10) is added into the elastin aqueous solution, and the mixture is filled with nitrogen gas at normal temperature and stirred for reaction for 3 hours in the dark. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and the purified protein solution was then lyophilized in a lyophilizerAnd (4) freeze-drying to obtain the modified elastin.

Example 6

This example provides a method for modifying photocrosslinkable elastin, comprising the following steps: 0.2g soluble elastin in 10mL pH 8.3 0.06M NaH ₂ PO ₄ The buffer solution is prepared into an elastin water solution. To the elastin aqueous solution was added 0.1g methacrylate-polyethylene glycol-N-hydroxysuccinimide (polyethylene glycol degree of polymerization 4), and the reaction was stirred at room temperature under nitrogen and in the dark for 0.5 hours. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin.

Example 7

This example provides a method for modifying photocrosslinkable elastin, comprising the following steps: 0.08g of soluble elastin was dissolved in 10mL of 0.01M phosphate physiological buffer pH 8.0 to prepare an aqueous elastin solution. 0.02g of acrylic ester-polyethylene glycol-N-hydroxysuccinimide (the polymerization degree of polyethylene glycol is 4) is added into the elastin aqueous solution, and the mixture is filled with nitrogen gas at normal temperature and stirred for reaction for 3 hours in the dark. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin. The reaction scheme of AC-PEG-NHS modified elastin is shown in figure 1.

Example 8

This example provides a method for modifying photocrosslinkable elastin, comprising the following steps: 0.15g of soluble elastin was dissolved in 10mL of 0.01M phosphate physiological buffer pH 8.3 to prepare an aqueous elastin solution. 0.1g of acrylic ester-polyethylene glycol-N-hydroxysuccinimide (the polymerization degree of polyethylene glycol is 20) is added into the elastin aqueous solution, and the mixture is filled with nitrogen gas at normal temperature and stirred for reaction for 0.5 hour in the dark. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin.

Example 9

The present embodiment provides a device capable ofThe modification method of the photocrosslinking elastin comprises the following steps: 0.1g soluble elastin in 10mL pH 8.3 0.06M NaH ₂ PO ₄ The buffer solution is prepared into an elastin water solution. 0.04g of acrylate-polyethylene glycol-N-hydroxysuccinimide (the polymerization degree of polyethylene glycol is 4) is added into the elastin aqueous solution, and the mixture is filled with nitrogen gas at normal temperature and stirred for reaction for 3 hours in the dark. The reaction was dialyzed in a dialysis bag with a molecular weight cut-off of 3kDa for 48 hours, and then the purified protein solution was lyophilized in a lyophilizer to obtain modified elastin.

The modified elastin prepared in any of examples 1-9 was characterized using a fourier transform infrared spectrometer. The modified elastin can be found to have new absorption peaks at 892 and 1069 wavenumbers, which are the out-of-plane bending vibration of C-H carbon-carbon double bond on acrylic acid and the C-O-C stretching vibration absorption peak on polyethylene glycol (PEG), respectively, and the successful modification is proved, as shown in FIG. 2.

Example 10

This example provides a method for preparing a photocrosslinkable elastin hydrogel: in examples 1 to 9, 200mg of the lyophilized modified elastin prepared in any of the examples was dissolved in 2mL of phosphate physiological buffer solution (pH7.4) containing blue light initiator lithium phenyl-2, 4, 6-trimethylbenzoylphosphinate (0.3mg/mL) to prepare 100mg/mL of elastin hydrogel precursor solution. Dropping 100uL of the solution into a fixed mould by using a pipette, and irradiating for 3 minutes by using a bluelight instrument (with the wavelength of 405nm) to form the hydrogel in situ. FIG. 3 is an appearance of the elastin hydrogel prepared, the hydrogel diameter being 5 mm.

Example 11

This example provides a method for preparing a photocrosslinkable elastin hydrogel: in examples 1 to 9, 200mg of the lyophilized modified elastin prepared in any of the examples was dissolved in 2mL of deionized water containing the blue light initiator lithium phenyl-2, 4, 6-trimethylbenzoylphosphinate (0.5mg/mL) to prepare a 100mg/mL elastin hydrogel precursor solution. Dropping 100uL of the solution into a fixed mould by using a pipette, and irradiating for 3 minutes by using a bluelight instrument (with the wavelength of 405nm) to form the hydrogel in situ.

Example 12

This example provides a method for preparing a photocrosslinkable elastin hydrogel: in examples 1 to 9, 200mg of the lyophilized modified elastin prepared in any of the examples was dissolved in 2mL of phosphate physiological buffer solution (pH7.4) containing UV initiator 2-hydroxy-4' - (2-hydroxyethoxy) -2-methylpropiophenone (0.5mg/mL) to prepare a 100mg/mL elastin hydrogel precursor solution. Dropping 100uL of the solution into a fixed mould by using a pipette, and irradiating for 4 minutes by using an ultraviolet instrument (the wavelength is 365nm) to form hydrogel in situ.

Example 13

This example provides a method for preparing a photo-crosslinkable elastin/gelatin composite hydrogel: in examples 1 to 9, 100mg of the lyophilized modified elastin prepared in any of the examples was dissolved in 2mL of phosphate physiological buffer solution (pH7.4) containing 2-hydroxy-4' - (2-hydroxyethoxy) -2-methylpropiophenone (0.5mg/mL) as a UV initiator to prepare a 100mg/mL elastin hydrogel precursor solution. 100mg of methacrylated gelatin is added to the precursor solution and dissolved to prepare 200mg/mL of composite hydrogel precursor solution. Dropping 100uL of the solution into a fixed mould by using a pipette, and irradiating for 4 minutes by using an ultraviolet instrument (the wavelength is 365nm) to form hydrogel in situ.

Example 14

This example provides a method for preparing a photo-crosslinkable elastin/hyaluronic acid composite hydrogel: in examples 1 to 9, 150mg of the lyophilized modified elastin prepared in any of the examples was dissolved in 2mL of deionized water containing the blue light initiator lithium phenyl-2, 4, 6-trimethylbenzoylphosphinate (0.5mg/mL) to prepare a 150mg/mL elastin hydrogel precursor solution. 50mg of methacrylated hyaluronic acid is added to the precursor solution and dissolved to prepare 200mg/mL of composite hydrogel precursor solution. Dropping 100uL of the solution into a fixed mould by using a pipette, and irradiating for 3 minutes by using a bluelight instrument (with the wavelength of 405nm) to form the hydrogel in situ.

Test example 1

The wound surface with the diameter of 10mm is prepared by taking the full-thickness skin defect wound surface of the back of a C57BL/6 mouse as a model, and a layer of silica gel ring is arranged around the wound surface of all the mice to prevent skin contraction. The allogenic control was used with a sample size of 3. Grouping experiments: the elastin hydrogel group prepared by the invention is a commercialized acrylic acid modified gelatin group and a blank control group. Treatment mode of experimental group: in examples 1 to 9, 200mg of the lyophilized modified elastin prepared in any of the examples was dissolved in 2mL of a physiological buffer solution of phosphate (pH7.4) containing the blue light initiator lithium phenyl-2, 4, 6-trimethylbenzoylphosphinate (0.3mg/mL) to prepare a 100mg/mL elastin hydrogel precursor solution, and filtered and sterilized in a 0.22 μm sterile filter. 100uL of the sterilized elastin hydrogel precursor solution is dripped to the wound surface by a liquid-transferring gun, and the in-situ gelling is carried out after the irradiation for 3 minutes by a blue light instrument. The commercial acrylic acid modified gelatin group has the same experimental procedures of protein concentration and crosslinking as the elastin group. The control group was not treated at all. The wound was then bandaged with 3M breathable medical tape and secured with an elastic bandage to prevent the tape from falling off. The wound surface was photographed 3, 7 and 14 days after surgery to observe the healing condition of the wound. Wound tissue samples were taken at each time point for HE staining, masson staining. The wound pictures show that the healing speed of the elastin hydrogel group is obviously faster than that of the blank control group, and the elastin hydrogel group has no obvious difference with the commercial acrylic acid modified gelatin group. HE staining results suggested that the elastin hydrogel group had more angiogenesis than the other two groups, indicating that the elastin hydrogel of the present invention was more conducive to dermal regeneration. The masson staining results showed that the elastin containing group produced more, denser, and stronger collagen fibers than the other two groups. Therefore, the elastin hydrogel prepared by the invention is an ideal wound repair material.

The above description is only an example of the present invention, but the present invention is not limited to the above example, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention and are equivalent to each other are included in the protection scope of the present invention.

Claims

1. A preparation method of an elastin hydrogel material for wound repair is characterized by comprising the following steps:

(2) adding 0.1-1 part by mass of acrylate-polyethylene glycol-N-hydroxysuccinimide or methacrylate-polyethylene glycol-N-hydroxysuccinimide into the elastin aqueous solution, and reacting under the conditions of filling nitrogen at normal temperature and keeping out of the sun to obtain modified elastin;

(4) dissolving 0.5-20 parts by mass of the dry modified elastin in 100 parts of deionized water or phosphate physiological buffer solution, adding a photoinitiator, and then irradiating under a light source with a certain wavelength to prepare the elastin hydrogel material.

2. The method for preparing an elastin hydrogel material for wound repair as claimed in claim 1, wherein: in the step (1), the soluble elastin is derived from hydrolysate of insoluble elastin in fish or mammal tissues; the buffer solution is 0.01M phosphate physiological buffer solution or 0.01-0.06M NaH ₂ PO ₄ A buffer solution; the pH value of the buffer solution is 8.0-8.3.

3. The method for preparing an elastin hydrogel material for wound repair as claimed in claim 1, wherein: in the step (2), the polymerization degree of PEG in the acrylate-polyethylene glycol-N-hydroxysuccinimide or the methacrylate-polyethylene glycol-N-hydroxysuccinimide is 4-20.

4. The method for preparing an elastin hydrogel material for wound repair as claimed in claim 1, wherein: in the step (2), the reaction time of the acrylate-polyethylene glycol-N-hydroxysuccinimide or the methacrylate-polyethylene glycol-N-hydroxysuccinimide with the elastin water solution is 0.5-3 hours.

5. The method for preparing an elastin hydrogel material for wound repair as claimed in claim 1, wherein: in the step (4), the wavelength of the irradiation light is 365nm or 405 nm; when 365nm ultraviolet light is adopted for irradiation, the corresponding photoinitiator is an ultraviolet photoinitiator; when 405nm blue light is adopted for irradiation, the corresponding photoinitiator is a blue light initiator; the final concentration of the added initiator is 0.01-0.5%.

6. The method for preparing an elastin hydrogel material for wound repair according to claim 5, wherein the method comprises the following steps: the ultraviolet initiator is 2-hydroxy-4' - (2-hydroxyethoxy) -2-methyl propiophenone; the blue light initiator is phenyl-2, 4, 6-trimethyl benzoyl lithium phosphinate.

7. The method for preparing an elastin hydrogel material for wound repair as claimed in claim 1, wherein: in the step (4), the photocrosslinking irradiation time is 0.5-10 minutes.

8. The method for preparing an elastin hydrogel material for wound repair as claimed in claim 1, wherein: in the step (4), 0.1-10 parts by mass of other water-soluble polymers with photo-crosslinking functional groups can be added, the water-soluble polymers and 0.5-10 parts by mass of dry modified elastin are dissolved in 100 parts of deionized water or phosphate physiological buffer solution, 0.01-0.5 part of photoinitiator is added, and then the elastin composite hydrogel material is formed by irradiating for a certain time under a light source with a certain wavelength.

9. The method for preparing an elastin hydrogel material for wound repair according to claim 8, wherein the method comprises the following steps: the other water-soluble polymers with photo-crosslinking functional groups comprise protein polymers, polysaccharide polymers, other synthetic water-soluble polymers, or any combination of the above polymers.

10. An elastin hydrogel material for wound repair, which is characterized in that: prepared by the method of any one of claims 1 to 9.