CN114870063B - Modified glucan nano microsphere composite dressing and preparation method thereof - Google Patents

Modified glucan nano microsphere composite dressing and preparation method thereof Download PDF

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Publication number
CN114870063B
CN114870063B CN202210602814.7A CN202210602814A CN114870063B CN 114870063 B CN114870063 B CN 114870063B CN 202210602814 A CN202210602814 A CN 202210602814A CN 114870063 B CN114870063 B CN 114870063B
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layer
modified
solution
microspheres
sodium alginate
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CN114870063A (en
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郝好杰
王雪蒙
李梓源
易军
陈惠华
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Beijing Rmbio Tech Co ltd
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Beijing Rmbio Tech Co ltd
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    • B32B5/02—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by structural features of a fibrous or filamentary layer
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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • Materials Engineering (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Toxicology (AREA)
  • Medicinal Preparation (AREA)
  • Materials For Medical Uses (AREA)
  • Nonwoven Fabrics (AREA)

Abstract

The invention discloses a modified glucan nanoparticle composite dressing, which comprises an active layer, a waterproof layer, a fungus isolation layer and a protective layer, wherein the active layer is a skin-friendly layer, the waterproof layer and the fungus isolation layer are intermediate layers, and the protective layer is an outermost layer; the active layer is a sodium alginate-polyvinyl alcohol composite fiber film with modified glucan nano-microspheres dispersed in the fiber, and the modified glucan nano-microspheres are the glucan nano-microspheres coated with growth factors.

Description

Modified glucan nano microsphere composite dressing and preparation method thereof
Technical Field
The invention relates to the field of medical materials, in particular to a dressing with a promoting effect on wound healing.
Background
In daily life, skin is often damaged and ulcerated, such as bruises, wounds, burns and scalds, diabetic feet, aged legs and feet, postoperative incision infections, and the like. Some open wounds do not heal easily and are susceptible to secondary infections, for which patients suffer great pain. Therefore, the selection of an ideal wound dressing is of great significance and has great market demands. The ideal dressing should have the following characteristics: can ensure the sterile environment of the wound, has certain air permeability and water absorbability, and has certain mechanical strength and the like. The ideal wound dressing not only can relieve pain for patients, but also can accelerate the healing speed of wounds.
The wound healing process is a complex and precise physiological process, and involves the interaction of various cells and growth factors, and the growth factors have various biological effects of inducing and promoting cell proliferation and differentiation, stimulating angiogenesis, promoting collagen synthesis and the like, so that the wound healing process has important significance for cell proliferation and repair at the wound. Many products and related studies use growth factors in adjuvants as release agents for slow release systems. The therapeutic effect of promoting wound repair and healing is achieved by slowly releasing the growth factors at the wound.
The electrostatic spinning technology is a common preparation technology of wound dressing, and the fiber prepared by electrostatic spinning has the advantages of large surface area, high porosity, good air permeability and hygroscopicity, and provides a good environment for wound repair and healing. However, the complex and fragile advanced conformation of the growth factor is extremely easy to denature and inactivate due to the severe conditions in the preparation process of the tissue engineering scaffold, so that the growth factor is directly mixed into an electrospinning solution for electrospinning, and the inactivation of the active factor is easily caused by an organic solvent, and the problem restricts the combination of the growth factor and the technology for preparing the wound dressing by electrospinning.
Disclosure of Invention
Therefore, the application provides a modified glucan nanoparticle composite dressing and a preparation method thereof, so as to solve the problem that the growth factors in the prior art are difficult to be effectively combined with the technology of preparing the wound dressing by electrostatic spinning.
In order to achieve the above object, the present application provides the following technical solutions:
in a first aspect, the application provides a modified glucan nanoparticle composite dressing, which comprises an active layer, a waterproof layer, a fungus isolation layer and a protective layer, wherein the active layer is a skin-friendly layer, the waterproof layer and the fungus isolation layer are intermediate layers, and the protective layer is an outermost layer; the active layer is a sodium alginate-polyvinyl alcohol composite fiber film with modified glucan nano-microspheres dispersed in the fiber, and the modified glucan nano-microspheres are the glucan nano-microspheres coated with growth factors.
Alternatively, the growth factor includes hepatocyte growth factor, platelet-derived growth factor, vascular endothelial growth factor, transforming growth factor, epidermal growth factor, fibroblast growth factor, and nerve growth factor.
Optionally, the waterproof layer is specifically a non-woven fabric, and is used for isolating the water absorption layer and the fungus isolation layer, preventing the fungus isolation layer from being impregnated with seepage liquid, and protecting the fungus isolation layer.
Optionally, the bacteria-isolating layer is specifically a melt-blown non-woven fabric made of polypropylene special resin with high melt index, and static electricity is generated by the bacteria-isolating layer under the action of friction force, so that fine particles are adsorbed, and the bacteria filtering effect is achieved.
Optionally, the protective layer is specifically a waterproof treated polypropylene nonwoven fabric, which is used to block the entry of droplets and liquids so that the bacteria-barrier layer is in a dry environment.
In a second aspect, the present application provides a method for preparing a modified glucose nanoparticle composite dressing, comprising the steps of:
s1, preparation of growth factors:
performing subculture on umbilical cord mesenchymal stem cells, subculturing to 3-6 generations, culturing for 3-5 days each generation, and collecting culture medium supernatant after culturing the cells; concentrating, sub-packaging and freeze-drying the collected culture medium supernatant under aseptic conditions to obtain growth factors, and hermetically preserving for later use;
s2, preparing modified glucan nanometer microspheres:
s2.1, respectively dissolving glucan and PEG6000 into 5% (w/w) solutions, uniformly mixing the two solutions according to a certain proportion, adding a proper amount of growth factors prepared in the S1, and continuously and uniformly mixing to obtain a raw material solution;
s2.2, carrying out freeze drying treatment on the raw material solution to obtain freeze-dried raw material powder;
s2.3, dissolving the freeze-dried powder in dichloromethane, uniformly mixing, centrifuging to remove upper liquid, collecting bottom powder, and repeating for 3-5 times until the liquid is completely removed;
s2.4, drying the powder obtained in the step S2.3 at 40 ℃ to obtain modified glucose nano-microspheres with diameters of 0.1-5 mu m, and sealing and preserving the modified glucose nano-microspheres for later use;
s3, preparing a sodium alginate-polyvinyl alcohol composite fiber membrane with modified glucan nano microspheres dispersed therein:
s3.1, mixing and uniformly stirring a polyvinyl alcohol solution and a sodium alginate solution, then adding a proper amount of modified glucose nano-microspheres prepared in the step S2.4, and uniformly stirring to obtain a spinning solution;
s3.2, spinning by taking a spinning solution as a raw material, and spinning to obtain a raw material fiber membrane;
s3.3, performing cross-linking treatment on the raw material fiber membrane to obtain a sodium alginate-polyvinyl alcohol composite fiber membrane with modified glucan nano-microspheres dispersed in the fiber;
s4, assembling:
respectively taking non-woven fabrics with the same shape and size, melt-blown non-woven fabrics made of polypropylene special resin with high melt index, waterproof polypropylene non-woven fabrics and sodium alginate-polyvinyl alcohol composite fiber films with modified glucan nano-microspheres dispersed in the fibers prepared in the step S3, placing the sodium alginate-polyvinyl alcohol composite fiber films with the modified glucan nano-microspheres dispersed in the fibers in the lowest layer to serve as skin-friendly layers, sequentially stacking the non-woven fabrics and the melt-blown non-woven fabrics made of polypropylene special resin with high melt index on the skin-friendly layers to serve as intermediate layers, and placing the waterproof polypropylene non-woven fabrics on the intermediate layers to serve as the outermost layers; the method comprises the steps of sewing a skin-friendly layer, a middle layer and an outermost layer together by using hydrophobic polypropylene fibers which are not adhered to wounds as sewing materials through a needling process to obtain the modified glucose nanometer microsphere composite dressing, wherein a sodium alginate-polyvinyl alcohol composite fiber film with modified glucan nanometer microspheres dispersed in the fibers is used as an active layer, a non-woven fabric and a melt-blown non-woven fabric made of polypropylene special resin with high melt index are respectively used as a waterproof layer and a bacteria-isolating layer, and the polypropylene non-woven fabric subjected to waterproof treatment is used as a protective layer.
Optionally, in S2.1:
the addition amount of the growth factors is the growth factors: dextran: PEG6000 = 1:800-1000: 1000-3000 (w/w/w). A step of
Optionally, in S3.1:
the polyethylene solution is specifically 5% -15% of polyethylene water solution, and the sodium alginate solution is specifically 0.5% -5% of sodium alginate water solution;
the mixing ratio of the polyethylene solution to the sodium alginate solution is 1:2-5:1;
the addition amount of the modified glucose nano-microsphere is 1-10% of the mass fraction of the mixed solution of the polyethylene solution and the sodium alginate solution.
Optionally, in S3.2, the spinning is specifically:
and (3) spinning the spinning solution by electrostatic spinning equipment, wherein the voltage is regulated to 10-30kv, the distance between a spinning nozzle and a receiving plate is 5-15cm, the flow rate is 0.1-1mL/h, and the spinning temperature is 20-30 ℃.
Optionally, in S3.3, the crosslinking treatment specifically includes:
CaCl with concentration of 1% -5% is prepared 2 The raw material fiber film prepared in S3.2 is put into CaCl 2 In the absolute ethyl alcohol solution of (2), crosslinking is carried out in the room temperature environment, and the crosslinking time is 1-12 h; after the crosslinking is completed, the CaCl remained on the surface of the crosslinked raw material fiber membrane is removed by absolute ethyl alcohol 2 And drying at room temperature to obtain the sodium alginate-polyvinyl alcohol composite fiber membrane with the modified glucan nano-microspheres dispersed in the fiber.
Compared with the prior art, the application has the following beneficial effects:
(1) According to the modified glucan nanoparticle composite dressing, the active layer is the sodium alginate-polyvinyl alcohol composite fiber film wrapping the glucan nanoparticle of the growth factor, the growth factor is wrapped in the glucan nanoparticle, and then the microsphere is dispersed in the composite fiber, so that the wrapping method can ensure the effective utilization of the growth factor and ensure the slow release of the growth factor, the growth factor is most effectively utilized at a wound, the glucan has good biocompatibility, the wound healing is promoted to a certain extent, the activity of various cells is enhanced, the wound healing is promoted effectively, and meanwhile, the wound dressing has an immunoregulation effect, can resist bacterial and fungal infection, and has a certain protection effect on the wound.
(2) The application provides a modified glucan nanometer microsphere composite dressing, the sodium alginate-polyvinyl alcohol composite fiber membrane that adopts becomes the hydrogel after absorbing the wound seepage liquid, is favorable to releasing the glucan nanometer microsphere of parcel growth factor and reaches the wound, accelerates the healing of wound, and the hydrogel can not scab the wound, has guaranteed the wettability and the comfort of wound, can also promote the healing of wound.
(3) The application provides a modified glucan nanometer microsphere composite dressing, waterproof layer, fungus-proof layer and protective layer effectually guaranteed the aseptic environment of wound, prevent wound secondary infection.
(4) The preparation method of the modified glucan nanoparticle composite dressing mainly adopts an electrostatic spinning technology, the electrostatic spinning technology is simple in operation process, and the prepared non-manufactured film has ultrahigh specific surface area and porosity, so that the preparation method is an ideal method for preparing the tissue engineering scaffold with surface activity; because the growth factors are wrapped in the glucan nanometer microspheres, the growth factors can be effectively wrapped in the fiber membrane during electrostatic spinning without damaging the activity of the fiber membrane, and the organic combination of the growth factors and the technology for preparing the wound dressing by electrostatic spinning is realized.
(5) The application provides a preparation method of modified glucan nanometer microsphere composite dressing adopts the acupuncture technology to make active layer, waterproof layer, fungus isolation layer and protective layer, makes there is sufficient clearance to carry out oxygen exchange between every layer of fibrous membrane, has guaranteed the gas permeability of dressing promptly, can guarantee again that the subsides between every layer of dressing are difficult for the slippage, have increased dressing and the degree of adherence and the comfort of surface of a wound.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings that are required to be used in the description of the embodiments of the present invention will be briefly described below. It will be apparent to those skilled in the art that the drawings in the following description are merely exemplary and that other drawings may be derived from the drawings provided without the inventive effort to those skilled in the art.
The following drawings are provided to enable those skilled in the art to understand and read the disclosure, and are not intended to limit the applicable scope of the present invention, and any modifications may be made without affecting the efficacy or reach of the invention.
Fig. 1 is a schematic structural diagram of a modified dextran nanoparticle composite dressing provided in embodiment 1 of the present application;
fig. 2 is a schematic microstructure diagram of a sodium alginate-polyvinyl alcohol composite fiber membrane in which modified dextran nanospheres are dispersed in the fiber in the modified dextran nanosphere composite dressing provided in embodiment 1 of the present application;
fig. 3 is a schematic structural diagram of an electrostatic spinning device used in the preparation method of the modified dextran nanoparticle composite dressing provided in embodiment 1 of the present application.
Drawings
In the figure: the composite dressing comprises a 1-active layer, a 2-waterproof layer, a 3-bacteria isolation layer, a 4-protective layer, a 5-modified glucan nano microsphere composite dressing, a 6-injection pump, a 7-electrospinning solution, an 8-high voltage power supply, a 9-receiving plate and 10-modified glucan nano microsphere.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
In the description of the present invention, the terms "comprises," "comprising," and any variations thereof, if any, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements but may include other steps or elements not expressly listed but inherent to such process, method, article, or apparatus or steps or elements added based on further optimization of the inventive concept.
Example 1
First, the embodiment provides a modified glucan nanoparticle composite dressing 5, which comprises an active layer 1, a waterproof layer 2, a fungus-isolating layer 3 and a protective layer 4, wherein the active layer 1 is a skin-friendly layer, the waterproof layer 2 and the fungus-isolating layer 3 are intermediate layers, and the protective layer 4 is an outermost layer; the active layer 1 is a sodium alginate-polyvinyl alcohol composite fiber film with modified glucan nano-microspheres dispersed in the fiber, and the modified glucan nano-microspheres are glucan nano-microspheres coated with growth factors; the waterproof layer 2 is made of common polypropylene non-woven fabrics, and mainly comprises an active layer 1 and a fungus-isolating layer 3 for isolating and absorbing seepage, so that the fungus-isolating layer 3 is prevented from being impregnated with seepage, and a certain protection effect is achieved on the fungus-isolating layer 3; the bacteria-isolating layer 3 is a melt-blown non-woven fabric made of special polypropylene resin with high melt index, which is economical and practical, and generates static electricity under the action of friction force, so that tiny particles are adsorbed, the bacteria-isolating layer 3 has the function of filtering bacteria, and the bacteria-isolating layer 3 provides a certain protection effect on the healing environment of wounds and prevents secondary infection of the wounds; the protective layer 4 is made of waterproof polypropylene non-woven fabric, and mainly has the effect of preventing spray, and ensures the dry environment of the fungus-isolation layer 3.
In this example, the growth factors used are various growth factors including Hepatocyte Growth Factor (HGF), platelet-derived growth factor (PDGF), vascular Endothelial Growth Factor (VEGF), transforming Growth Factor (TGF), epidermal Growth Factor (EGF), fibroblast Growth Factor (FGF), nerve Growth Factor (NGF) and the like.
Second, the embodiment provides a preparation method of a modified glucan nanoparticle composite dressing, which comprises the following steps:
(1) Respectively dissolving dextran and PEG6000 into 5% (w/w) solutions, uniformly mixing the two solutions according to a certain proportion, adding a certain amount of growth factors, and continuously uniformly mixing; (growth factor: dextran: PEG6000 = 1:1000:3000 (w/w)) subjecting the uniformly mixed solution to freeze-drying treatment, dissolving the freeze-dried powder in dichloromethane, uniformly mixing, centrifuging to remove the upper layer liquid, specifically PEG, and collecting the powder at the bottom of the centrifuge tube; repeating for 3-5 times, and completely eluting the PEG; drying the obtained powder at 40 ℃ at low temperature to obtain modified glucan nano-microspheres with diameters of 0.1-5 mu m, and sealing and preserving the modified glucan nano-microspheres for later use;
(2) Polyvinyl alcohol (PVA) is dissolved in water, stirred and dissolved for 4-6 hours at 90 ℃ to prepare a 5% PVA aqueous solution;
(3) Sodium alginate is dissolved in water to prepare 0.9% sodium alginate solution;
(4) Uniformly mixing and stirring the PVA aqueous solution obtained in the step (2) and the sodium alginate solution obtained in the step (3) according to the ratio of 1:1 to obtain a sodium alginate-polyvinyl alcohol solution;
(5) Adding 2% of modified glucan nano-microspheres prepared in the step (1) into the sodium alginate-polyvinyl alcohol solution, and uniformly stirring to prepare an electrospinning solution 7;
(6) Spinning the electrospinning solution 7 prepared in the step (5) through an electrospinning device; during spinning, the electrospinning solution 7 is added into the injection pump 6, the voltage of the high-voltage power supply 8 is regulated to 10-30kv, the distance between a spinning nozzle of the injection pump 6 and the receiving plate 9 is 5-15cm, the flow rate is 0.1-1mL/h, and the spinning temperature is 20-30 ℃; preparing a raw material fiber membrane by electrostatic spinning;
(7) CaCl is added with 2 Dissolving in absolute ethanol to obtain 2% CaCl 2 Is an absolute ethanol solution of (a);
placing the raw material fiber membrane in the step (6) into CaCl prepared in the step (7) 2 In the absolute ethyl alcohol solution of (2) and crosslinking is carried out in the room temperature environment, wherein the crosslinking time is 2h; after the crosslinking is completed, the CaCl remained on the surface of the crosslinked raw material fiber membrane is removed by absolute ethyl alcohol 2 Drying at room temperature to obtain a sodium alginate-polyvinyl alcohol composite fiber membrane with modified glucan nano-microspheres dispersed in the fiber as an active layer of the dressing;
(8) Taking common polypropylene non-woven fabrics with the same shape and size, melt-blown non-woven fabrics made of special polypropylene resin with high melt index and polypropylene non-woven fabrics subjected to waterproof treatment as a waterproof layer, a bacteria-isolating layer and a protective layer respectively, sequentially superposing the active layer prepared in the step (7) with the waterproof layer, the bacteria-isolating layer and the protective layer, taking hydrophobic polypropylene fibers which are not adhered to wounds as suture materials, and combining the active layer, the waterproof layer, the bacteria-isolating layer and the protective layer together by a needling process to prepare the modified glucan nanoparticle composite dressing; wherein the skin-friendly layer is an active layer, the middle layer is a waterproof layer and a bacteria-isolating layer, and the outermost layer is a protective layer.
Example 2
First, this embodiment provides a method for preparing a modified dextran nanoparticle composite dressing, which has steps substantially identical to those of the preparation method provided in embodiment 1, and is different in that:
in step (1), growth factors: dextran: PEG6000 = 1:800:1000 (w/w/w);
in the step (2), an 8% PVA aqueous solution is prepared;
in the step (3), preparing a 1.5% sodium alginate solution;
in the step (4), the mixing ratio of the PVA aqueous solution to the sodium alginate solution is 1.5:2;
in the step (5), the addition amount of the modified glucan nano microsphere is 10% of the mass fraction of the sodium alginate-polyvinyl alcohol solution;
in step (7), caCl 2 The concentration of the absolute ethanol solution of (2) is 3%;
in step (8), the crosslinking time was 4 hours.
Second, the embodiment provides a modified glucan nanoparticle composite dressing, which is prepared by the preparation method provided by the embodiment.
Example 3
First, this embodiment provides a method for preparing a modified dextran nanoparticle composite dressing, which has steps substantially identical to those of the preparation method provided in embodiment 1, and is different in that:
in step (1), growth factors: dextran: PEG6000 = 1:800:1000 (w/w/w);
in the step (2), a 13% PVA aqueous solution is prepared;
in the step (3), preparing a 1.2% sodium alginate solution;
in the step (4), the mixing ratio of the PVA aqueous solution to the sodium alginate solution is 1.5:1;
in the step (5), the addition amount of the modified glucan nano microsphere is 10% of the mass fraction of the sodium alginate-polyvinyl alcohol solution;
in step (7), caCl 2 The concentration of the absolute ethanol solution is 5%;
in step (8), the crosslinking time was 1h.
Second, the embodiment provides a modified glucan nanoparticle composite dressing, which is prepared by the preparation method provided by the embodiment.
The technical features of the above embodiments may be arbitrarily combined, and for brevity, all of the possible combinations of the technical features of the above embodiments are not described; these examples, which are not explicitly written, should also be considered as being within the scope of the present description.
The invention has been described above with particularity and detail in connection with general description and specific embodiments. It should be noted that it is obvious that several variations and modifications can be made to this specific embodiment without departing from the spirit of the present invention, which are all within the scope of protection of the present application. Accordingly, the scope of protection of the present application is to be determined by the claims appended hereto.

Claims (6)

1. The preparation method of the modified glucan nanoparticle composite dressing is characterized by comprising the following steps of:
s1, preparation of growth factors:
performing subculture on umbilical cord mesenchymal stem cells, subculturing to 3-6 generations, culturing for 3-5 days each generation, and collecting culture medium supernatant after culturing the cells; concentrating, sub-packaging and freeze-drying the collected culture medium supernatant under aseptic conditions to obtain growth factors, and hermetically preserving for later use;
s2, preparing modified glucan nanometer microspheres:
s2.1, respectively dissolving glucan and PEG6000 into 5% (w/w) solutions, uniformly mixing the two solutions according to a certain proportion, adding a proper amount of the growth factor prepared in the S1, and continuously and uniformly mixing to obtain a raw material solution;
s2.2, carrying out freeze drying treatment on the raw material solution to obtain freeze-dried raw material powder;
s2.3, dissolving the freeze-dried raw material powder in dichloromethane, uniformly mixing, centrifuging to remove upper liquid, collecting bottom powder, and repeating for 3-5 times until the liquid is completely removed;
s2.4, drying the powder obtained in the step S2.3 at the temperature of 40 ℃ to obtain modified glucose nano-microspheres with the diameter of 0.1-5 mu m, and sealing and preserving the modified glucose nano-microspheres for later use;
s3, preparing a sodium alginate-polyvinyl alcohol composite fiber membrane with modified glucan nano microspheres dispersed therein:
s3.1, mixing and uniformly stirring a polyvinyl alcohol solution and a sodium alginate solution, then adding a proper amount of the modified glucose nano-microspheres prepared in the step S2.4, and uniformly stirring to obtain a spinning solution;
s3.2, spinning by taking the spinning solution as a raw material, and spinning to obtain a raw material fiber membrane;
s3.3, performing cross-linking treatment on the raw material fiber membrane to obtain a sodium alginate-polyvinyl alcohol composite fiber membrane with modified glucan nano-microspheres dispersed in the fiber;
s4, assembling:
respectively taking common polypropylene non-woven fabrics with the same shape and size, melt-blown non-woven fabrics made of special high-melt-index polypropylene resin, waterproof-treated polypropylene non-woven fabrics and sodium alginate-polyvinyl alcohol composite fiber films with modified glucan nano-microspheres dispersed in the fibers, which are prepared in the step S3, placing the sodium alginate-polyvinyl alcohol composite fiber films with the modified glucan nano-microspheres dispersed in the fibers in the lowest layer as skin-friendly layers, sequentially stacking the common polypropylene non-woven fabrics and the melt-blown non-woven fabrics made of special high-melt-index polypropylene resin on the skin-friendly layers to serve as middle layers, and placing the waterproof-treated polypropylene non-woven fabrics on the middle layers to serve as the outermost layers; the method comprises the steps of sewing a skin-friendly layer, a middle layer and an outermost layer together by using hydrophobic polypropylene fibers which are not adhered to wounds as sewing materials through a needling process to obtain the modified glucose nano microsphere composite dressing, wherein a sodium alginate-polyvinyl alcohol composite fiber film with modified glucan nano microspheres dispersed in the fibers is used as an active layer, a waterproof layer and a bacteria-isolating layer are respectively used as common polypropylene non-woven fabrics and melt-blown non-woven fabrics made of high-melt-index polypropylene special resins, and the waterproof treated polypropylene non-woven fabrics are used as protective layers;
the modified glucan nanoparticle composite dressing comprises an active layer, a waterproof layer, a fungus isolation layer and a protective layer, wherein the active layer is a skin-friendly layer, the waterproof layer and the fungus isolation layer are intermediate layers, and the protective layer is an outermost layer; the active layer is a sodium alginate-polyvinyl alcohol composite fiber film with modified glucan nano-microspheres dispersed in the fiber, and the modified glucan nano-microspheres are glucan nano-microspheres coated with growth factors;
the growth factors include hepatocyte growth factor, platelet-derived growth factor, vascular endothelial growth factor, transforming growth factor, epidermal growth factor, fibroblast growth factor and nerve growth factor;
the bacteria-isolating layer is specifically a melt-blown non-woven fabric made of polypropylene special resin with high melt index, and static electricity is generated by the bacteria-isolating layer under the action of friction force, so that fine particles are adsorbed, and the bacteria filtering effect is achieved;
the waterproof layer is specifically a common polypropylene non-woven fabric and is used for isolating the active layer from the bacteria isolation layer and preventing the bacteria isolation layer from being impregnated with seepage liquid, so as to protect the bacteria isolation layer.
2. The method for preparing a modified glucan nanoparticle composite dressing according to claim 1, wherein the protective layer is specifically a polypropylene nonwoven fabric subjected to waterproof treatment, and is used for blocking the entry of droplets and liquid so as to enable the bacteria-isolating layer to be in a dry environment.
3. The method for preparing the modified glucose nanoparticle composite dressing according to claim 1, wherein in S2.1:
the growth factor: the glucan: PEG6000 = 1:800-1000: 1000 to 3000 (w/w/w).
4. The method for preparing the modified glucose nanoparticle composite dressing according to claim 1, wherein in S3.1:
the polyvinyl alcohol solution is specifically a 5% -15% polyvinyl alcohol aqueous solution, and the sodium alginate solution is specifically a 0.5% -5% sodium alginate aqueous solution;
the mixing ratio of the polyvinyl alcohol solution to the sodium alginate solution is 1:2-5:1;
the addition amount of the modified glucose nano-microspheres is 1-10% of the mass fraction of the mixed solution of the polyvinyl alcohol solution and the sodium alginate solution.
5. The preparation method of the modified glucose nanoparticle composite dressing according to claim 1, wherein in S3.2, the spinning specifically comprises:
and (3) spinning the spinning solution through electrostatic spinning equipment, wherein during spinning, the voltage is regulated to 10-30kv, the distance between a spinning nozzle and a receiving plate is 5-15cm, the flow rate is 0.1-1mL/h, and the spinning temperature is 20-30 ℃.
6. The method for preparing a modified glucose nanoparticle composite dressing according to claim 1, wherein in S3.3, the crosslinking treatment specifically comprises:
CaCl with concentration of 1% -5% is prepared 2 The raw material fiber film prepared in the step S3.2 is put into the CaCl 2 Crosslinking is carried out in the absolute ethyl alcohol solution at room temperature for 1-12 h; after the crosslinking is completed, the CaCl remained on the surface of the crosslinked raw material fiber membrane is removed by absolute ethyl alcohol 2 And drying at room temperature to obtain the sodium alginate-polyvinyl alcohol composite fiber membrane in which the modified glucan nano-microspheres are dispersed.
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