CN114862183A

CN114862183A - Nucleic acid purification control method, gene detection control method, and related apparatus

Info

Publication number: CN114862183A
Application number: CN202210475400.2A
Authority: CN
Inventors: 李立策; 刘小彬; 肖东
Original assignee: Chengdu Hanchen Guangyi Bioengineering Co ltd; Chengdu Hanchen Guangyi Technology Co ltd
Current assignee: Chengdu Hanchen Guangyi Bioengineering Co ltd; Chengdu Hanchen Guangyi Technology Co ltd
Priority date: 2022-04-29
Filing date: 2022-04-29
Publication date: 2022-08-05

Abstract

The application provides a nucleic acid purification control method, a gene detection control method and a related device, and relates to the technical field of gene detection. In the case of obtaining the respective nucleic acid purification strategy of at least one sample group to be detected comprising at least one biological sample to be processed of the same batch, can combine the respective working states of a plurality of nucleic acid purification cooperative modules included in the gene detection equipment for each sample group to be processed, calling at least one adaptive target nucleic acid purification cooperative module on a single sample group to be processed to sequentially execute each nucleic acid purification process included by the corresponding nucleic acid purification strategy, so that all the nucleic acid purification cooperative modules can mutually cooperate to carry out rapid automatic nucleic acid purification operation on a single biological sample, or a large number of biological samples are subjected to concurrent automatic nucleic acid purification operation, so that the nucleic acid purification efficiency of the samples is effectively improved, the gene detection efficiency is integrally improved, and the high-flux effect and the automatic effect of the gene detection operation of the samples are realized.

Description

Nucleic acid purification control method, gene detection control method, and related apparatus

Technical Field

The application relates to the technical field of gene detection, in particular to a nucleic acid purification control method, a gene detection control method and a related device.

Background

With the continuous development of molecular biotechnology, gene detection technology has been widely applied in the fields of clinical medicine, agricultural breeding, judicial identification, food safety and the like. The extraction and purification of nucleic acid are used as the basis of gene detection, and are important preconditions for improving the quality and efficiency of gene detection. In the face of ever-increasing gene detection requirements, how to effectively improve nucleic acid extraction and purification efficiency and further achieve automatic and high-throughput gene detection effects becomes an important technical problem to be solved urgently at present.

Disclosure of Invention

In view of the above, an object of the present application is to provide a nucleic acid purification control method and apparatus, a gene detection device, and a readable storage medium, which can perform rapid and automated nucleic acid purification operations on a single biological sample, and also perform concurrent automated nucleic acid purification operations on a large number of biological samples, thereby effectively improving the nucleic acid purification efficiency of the sample, improving the gene detection efficiency as a whole, and achieving a high-throughput effect and an automation effect of the gene detection operation.

In order to achieve the above purpose, the embodiments of the present application employ the following technical solutions:

in a first aspect, the present application provides a method for controlling nucleic acid purification, the method comprising:

obtaining nucleic acid purification strategies of at least one sample group to be processed of the same batch, wherein each sample group to be processed corresponds to one nucleic acid purification strategy, and each sample group to be processed comprises at least one biological sample to be processed;

and calling a target nucleic acid purification cooperative module on each sample group to be processed according to the respective working states of a plurality of nucleic acid purification cooperative modules with different functions, which are included in the gene detection equipment, to sequentially execute the nucleic acid purification processes included in the corresponding nucleic acid purification strategies, wherein each nucleic acid purification process of the same nucleic acid purification strategy corresponds to at least one target nucleic acid purification cooperative module.

In an alternative embodiment, each nucleic acid purification cooperation module executes the added processes of the process execution queue in sequence according to the process arrangement status of the process execution queue of the module, and deletes the executed processes of the process execution queue correspondingly;

in this case, the step of calling the target nucleic acid purification cooperative module to sequentially perform each nucleic acid purification process included in the corresponding nucleic acid purification strategy on the sample group to be processed according to the respective operating states of the plurality of nucleic acid purification cooperative modules having different functions included in the gene detection apparatus includes:

sequentially aiming at each nucleic acid purification process in a nucleic acid purification strategy corresponding to a single sample group to be processed, detecting the current working state of at least one target nucleic acid purification cooperation module executing the nucleic acid purification process;

and if the current working states of all the target nucleic acid purification cooperative modules are detected to be idle states, directly calling all the target nucleic acid purification cooperative modules to execute the nucleic acid purification process on the sample group to be processed in a mutually matched manner, wherein the process execution queue of each nucleic acid purification cooperative module in the idle state is an empty queue.

In an optional embodiment, the step of calling the target nucleic acid purification cooperative module to sequentially perform each nucleic acid purification process included in the corresponding nucleic acid purification strategy on the sample group to be processed according to the respective working states of the plurality of nucleic acid purification cooperative modules with different functions included in the genetic testing apparatus further includes:

if the current working state of at least one target nucleic acid purification cooperative module is detected to be busy, adding the nucleic acid purification process into the process execution queues of all the target nucleic acid purification cooperative modules, wherein the process execution queue of each nucleic acid purification cooperative module in the busy state is a non-empty queue;

and when the nucleic acid purification process is at the top position of the queue in the process execution queues of all the target nucleic acid purification cooperation modules, calling all the target nucleic acid purification cooperation modules to cooperatively execute the nucleic acid purification process on the sample group to be processed.

In an alternative embodiment, the step of adding the nucleic acid purification process to the process execution queue of each of the target nucleic acid purification coordination modules includes:

determining the execution priority of the nucleic acid purification process according to the purification operation completion status and/or process execution emergency status corresponding to the nucleic acid purification process;

adding the nucleic acid purification process to the process execution queue of each target nucleic acid purification coordination module, and performing priority rearrangement on all the unexecuted processes in the process execution queue of each target nucleic acid purification coordination module, wherein the unexecuted processes closer to the top position of the queue have higher execution priority.

In a second aspect, the present application provides a method for controlling gene testing, the method comprising:

calling an amplification reagent adding module included in the gene detection equipment to add a PCR amplification reaction reagent on a target PCR plate;

according to the nucleic acid purification control method in any one of the preceding embodiments, a plurality of nucleic acid purification coordination modules with different functions included in the genetic testing equipment are invoked to cooperatively execute corresponding nucleic acid purification processes on at least one sample group to be processed in the same batch, and the at least one sample group to be processed after nucleic acid purification is transferred to the target PCR plate, so as to obtain nucleic acid samples to be detected, wherein each sample group to be processed includes at least one biological sample to be processed;

calling a sample amplification processing module included in the gene detection equipment to enable all nucleic acid samples to be detected on the target PCR plate to complete gene amplification reaction under the action of the PCR amplification reaction reagent;

and calling a gene fluorescence detection module included in the gene detection equipment to perform fluorescence detection on all nucleic acid samples to be detected after gene amplification on the target PCR plate is completed, so as to obtain corresponding sample gene detection results.

In a third aspect, the present application provides a nucleic acid purification control apparatus comprising:

the purification strategy acquisition module is used for acquiring nucleic acid purification strategies of at least one to-be-processed sample group of the same batch, wherein each to-be-processed sample group corresponds to one nucleic acid purification strategy, and each to-be-processed sample group comprises at least one to-be-processed biological sample;

and the purification component calling module is used for calling a target nucleic acid purification cooperation module on each sample group to be processed according to the respective working states of a plurality of nucleic acid purification cooperation modules with different functions, which are included in the gene detection equipment, so as to sequentially execute each nucleic acid purification process included in the corresponding nucleic acid purification strategy, wherein each nucleic acid purification process of the same nucleic acid purification strategy corresponds to at least one target nucleic acid purification cooperation module.

In an alternative embodiment, each nucleic acid purification coordination module sequentially executes the added processes of the process execution queue according to the process arrangement status of its own process execution queue, and deletes the executed processes of the process execution queue accordingly, where the purification component calling module includes:

the working state detection submodule is used for sequentially detecting the current working state of at least one target nucleic acid purification cooperative module executing the nucleic acid purification process aiming at each nucleic acid purification process in the nucleic acid purification strategy corresponding to a single sample group to be processed;

and the purification procedure execution submodule is used for directly calling all the target nucleic acid purification coordination modules to be mutually matched to execute the nucleic acid purification procedure on the sample group to be processed if the working state detection submodule detects that the current working states of all the target nucleic acid purification coordination modules are idle states, wherein the procedure execution queue of each nucleic acid purification coordination module in the idle state is an empty queue.

In an alternative embodiment, the purification component invoking module further comprises:

a purification process loading sub-module, configured to add the nucleic acid purification process to the respective process execution queues of all target nucleic acid purification cooperative modules if the working state detection sub-module detects that the current working state of at least one target nucleic acid purification cooperative module is a busy state, where the process execution queue of each nucleic acid purification cooperative module in the busy state is a non-empty queue;

and the purification procedure execution sub-module is further configured to invoke all the target nucleic acid purification coordination modules to cooperatively execute the nucleic acid purification procedure on the sample group to be processed when the nucleic acid purification procedure is at the top end position of the queue in the procedure execution queues of all the target nucleic acid purification coordination modules.

In an alternative embodiment, the purification process loading sub-module comprises:

a process priority determining unit for determining an execution priority of the nucleic acid purification process based on a completion status of the nucleic acid purification operation and/or an emergency status of process execution corresponding to the nucleic acid purification process;

and a process adding and rearranging unit for adding the nucleic acid purification process to the process execution queue of each target nucleic acid purification coordination module and rearranging the priority of all the unexecuted processes in the process execution queue of each target nucleic acid purification coordination module, wherein the more the unexecuted processes are closer to the top position of the queue, the higher the execution priority is.

In a fourth aspect, the present application provides a gene testing control apparatus, comprising:

the reagent adding and calling module is used for calling an amplification reagent adding module included in the gene detection equipment to add a PCR amplification reaction reagent on the target PCR plate;

the nucleic acid purification control device in any one of the preceding embodiments, configured to invoke a plurality of nucleic acid purification coordination modules with different functions included in the genetic testing equipment to perform, in coordination with each other, a corresponding nucleic acid purification process on at least one sample group to be processed of the same batch, and transfer the at least one sample group to be processed after nucleic acid purification to the target PCR plate, so as to obtain nucleic acid samples to be detected, where each sample group to be processed includes at least one biological sample to be processed;

the amplification processing calling module is used for calling the sample amplification processing module included by the gene detection equipment to enable all nucleic acid samples to be detected on the target PCR plate to complete gene amplification reaction under the action of the PCR amplification reaction reagent;

and the gene detection calling module is used for calling the gene fluorescence detection module included in the gene detection equipment to perform fluorescence detection on all nucleic acid samples to be detected on the target PCR plate after gene amplification is completed, so as to obtain corresponding sample gene detection results.

In a fifth aspect, the present application provides a gene detection apparatus, including a processor, a memory, an amplification reagent adding module, a sample amplification processing module, a gene fluorescence detection module, and a plurality of nucleic acid purification cooperative modules with different functions, where the plurality of nucleic acid purification cooperative modules cooperatively perform a nucleic acid purification operation on a biological sample, the amplification reagent adding module is configured to add a PCR amplification reaction reagent to a PCR plate, the sample amplification processing module is configured to prompt the nucleic acid sample to complete a gene amplification reaction under the action of the PCR amplification reaction reagent, and the gene fluorescence detection module is configured to perform fluorescence detection on the nucleic acid sample after the gene amplification is completed, so as to obtain a corresponding sample gene detection result;

the memory stores a computer program executable by the processor, and the processor may execute the computer program to call the plurality of nucleic acid purification cooperation modules to implement the nucleic acid purification control method according to any one of the foregoing embodiments, or call the amplification reagent addition module, the sample amplification processing module, the genetic fluorescence detection module, and the plurality of nucleic acid purification cooperation modules to implement the genetic detection control method according to the foregoing embodiments.

In a sixth aspect, the present application provides a readable storage medium having stored thereon a computer program which, when executed by a processor, implements the nucleic acid purification control method according to any one of the preceding embodiments or the gene detection control method according to the preceding embodiments.

Therefore, the beneficial effects of the embodiment of the application can include the following:

under the condition of obtaining the nucleic acid purification strategy of at least one to-be-processed sample group comprising at least one to-be-processed biological sample in the same batch, the method can be used for calling at least one adaptive target nucleic acid purification cooperation module on a single to-be-processed sample group to sequentially execute each nucleic acid purification process included by the corresponding nucleic acid purification strategy by combining the respective working states of a plurality of nucleic acid purification cooperation modules included by the gene detection equipment according to each to-be-processed sample group, so that all the nucleic acid purification cooperation modules can be used for carrying out rapid automatic nucleic acid purification operation on the single biological sample in a mutual cooperation mode or carrying out concurrent automatic nucleic acid purification operation on a large number of biological samples, the nucleic acid purification efficiency of the sample is effectively improved, the gene detection efficiency is integrally improved, and the high-flux effect and the automation effect of the gene detection operation are realized.

In order to make the aforementioned objects, features and advantages of the present application more comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic diagram showing the constitution of a gene assaying device according to an embodiment of the present invention;

FIG. 2 is a schematic flow chart of a method for controlling nucleic acid purification according to an embodiment of the present disclosure;

FIG. 3 is a flowchart illustrating one of the sub-steps included in step S220 in FIG. 2;

FIG. 4 is a second schematic flowchart illustrating the sub-steps included in step S220 in FIG. 2;

FIG. 5 is a schematic flow chart of the sub-steps included in sub-step S223 in FIG. 4;

FIG. 6 is a schematic flow chart of a gene detection control method provided in the embodiments of the present application;

FIG. 7 is a schematic diagram showing the composition of a nucleic acid purification control apparatus according to an embodiment of the present application;

FIG. 8 is a schematic diagram of the purification component invocation module of FIG. 7;

FIG. 9 is a schematic diagram of the loading submodule of the purification process of FIG. 8;

FIG. 10 is a schematic diagram showing the configuration of a gene detection control apparatus according to an embodiment of the present application.

Icon: 10-a gene detection device; 11-a memory; 12-a processor; 13-nucleic acid purification synergy module; 14-amplification reagent addition module; 15-a sample amplification processing module; 16-gene fluorescence detection module; 100-nucleic acid purification control means; 110-a purification strategy acquisition module; 120-purification component calling module; 121-a working state detection submodule; 122-purification process execution submodule; 123-purification process loading submodule; 1231-process priority determination unit; 1232-Process addition of rearrangement units; 300-a gene detection control device; 310-reagent addition calling module; 320-an amplification processing calling module; 330-Gene detection calling Module.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, as generally described and illustrated in the figures herein, could be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present application, it is to be understood that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.

Referring to fig. 1, fig. 1 is a schematic diagram illustrating a gene detecting apparatus 10 according to an embodiment of the present disclosure. In the embodiment of the present application, the genetic testing apparatus 10 can be used for performing a sample nucleic acid purification operation on at least one biological sample to be processed, and performing PCR amplification and fluorescence detection on the nucleic acid sample obtained by purification, so as to obtain a genetic testing result of the nucleic acid sample. In this process, the gene detection device 10 can perform rapid automatic nucleic acid purification operation on a single biological sample, or perform concurrent automatic nucleic acid purification operation on a large number of biological samples, so as to improve the hardware resource utilization rate of the device and ensure the nucleic acid purification efficiency of the biological samples, and on the other hand, can integrally control the cooperative execution of automatic operation functions such as sample nucleic acid purification, PCR amplification reaction system configuration, PCR amplification reaction, PCR fluorescence detection and the like in the gene detection process according to the nucleic acid purification operation progress of the biological samples, thereby further improving the gene detection efficiency of the sample and realizing the high flux effect and the automatic effect of the gene detection operation. The biological sample may include biological macromolecules, blood, cells, tissues, organs, and other samples of an organism.

In this embodiment, the gene assaying device 10 may include a memory 11, a processor 12, an amplification reagent adding module 14, a sample amplification processing module 15, a gene fluorescence detecting module 16, a gene assaying control apparatus 300, and a plurality of functionally different nucleic acid purification coordination modules 13. Wherein, the memory 11, the processor 12, the amplification reagent adding module 14, the sample amplification processing module 15, the gene fluorescence detection module 16 and the plurality of nucleic acid purification cooperation modules 13 are electrically connected to each other directly or indirectly to realize data transmission or interaction. For example, the memory 11, the processor 12, the amplification reagent adding module 14, the sample amplification processing module 15, the gene fluorescence detection module 16, and the nucleic acid purification coordination modules 13 may be electrically connected to each other through one or more communication buses or signal lines.

In this embodiment, the Memory 11 may be, but is not limited to, a Random Access Memory (RAM), a Read Only Memory (ROM), a Programmable Read-Only Memory (PROM), an Erasable Read-Only Memory (EPROM), an electrically Erasable Read-Only Memory (EEPROM), and the like. The memory 11 is used for storing a computer program, and the processor 12 can execute the computer program after receiving an execution instruction.

In this embodiment, the processor 12 may be an integrated circuit chip having signal processing capabilities. The Processor 12 may be a general-purpose Processor including at least one of a Central Processing Unit (CPU), a Graphics Processing Unit (GPU), a Network Processor (NP), a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), an off-the-shelf programmable gate array (FPGA) or other programmable logic device, a discrete gate or transistor logic device, and discrete hardware components. The general purpose processor may be a microprocessor or the processor may be any conventional processor or the like that implements or executes the methods, steps and logic blocks disclosed in the embodiments of the present application.

In this embodiment, a plurality of the nucleic acid purification cooperative modules 13 cooperate with each other to realize an automated nucleic acid purification operation of a biological sample to be processed. Wherein, a plurality of the nucleic acid purification cooperation modules 13 can respectively execute one or more operations related to nucleic acid purification function in an automated gene detection process, and specifically, the operations of the hardware modules related to the nucleic acid purification cooperation modules 13 in the automated gene detection process include: extracting one or more biological samples to be processed in the same group into corresponding octal tubes, adding a nucleic acid purification reagent into the octal tubes containing the biological samples to be processed, adding an internal reference reagent into the octal tubes containing the biological samples to be processed, moving the octal tubes added with relevant reagents (including the purification reagent and/or the internal reference reagent) to an oscillation cracking operation area, adding a lysis solution and a magnetic bead solution into the octal tubes bearing the biological samples to be processed in the oscillation cracking operation area, heating and oscillating the octal tubes added with the lysis solution and the magnetic bead solution in the oscillation cracking operation area, adding a cleaning solution into the octal tubes after heating and oscillating, extracting the cleaning solution adsorbed with gene sample impurities in the octal tubes, adding an eluent into the octal tubes, extracting sample nucleic acids of the purified biological samples to be processed from the surfaces of the magnetic beads in the octal tubes, and transferring the sample nucleic acids to a PCR (PolymerChase in Reaction, polymerase chain reaction) plate and transferring the PCR plate loaded with sample nucleic acid of the biological sample to be processed to an amplification zone, etc.

In this embodiment, the amplification reagent adding module 14 is used for injecting a PCR reaction reagent into the PCR plate, so that the nucleic acid sample to be detected contained in the PCR plate can be subjected to gene amplification under the action of the PCR reaction reagent.

In this embodiment, the sample amplification processing module 15 is used to enable the sample nucleic acid (i.e. the nucleic acid sample to be detected) of the biological sample to be processed contained in the PCR plate to complete a gene amplification reaction under the action of the PCR amplification reaction reagent. The sample amplification processing module 15 controls the PCR amplification reaction temperature of the biological sample nucleic acid to be processed by performing metal bath on the PCR plate to which the PCR amplification reaction reagent and the sample nucleic acid of the biological sample to be processed are added, so as to realize the "denaturation-annealing-extension" cycle of the gene in the biological sample nucleic acid to be processed, and amplify the specific gene in the biological sample nucleic acid to be processed.

In this embodiment, the gene fluorescence detection module 16 is configured to perform fluorescence detection on sample nucleic acid of the to-be-processed biological sample placed on the PCR plate after the PCR amplification reaction is completed, so as to obtain a gene detection result corresponding to the sample nucleic acid.

In this embodiment, the genetic testing control apparatus 300 may comprise a nucleic acid purification control apparatus 100, wherein the nucleic acid purification control apparatus 100 comprises at least one software function module capable of being stored in the memory 11 in the form of software or firmware or being solidified in the operating system of the genetic testing device 10. The processor 12 can be used to execute executable modules stored in the memory 11, such as software functional modules and computer programs included in the nucleic acid purification control apparatus 100. The gene detection device 10 can perform rapid and automatic nucleic acid purification operation on a single biological sample through the nucleic acid purification control device 100, or perform concurrent and automatic sample nucleic acid purification operation on a large number of biological samples, so that the hardware resource utilization rate of the device is improved, the sample nucleic acid purification efficiency of the biological samples is ensured, and basic conditions are provided for realizing high-throughput and automatic gene detection operation.

In this embodiment, the genetic testing control apparatus 300 may further include at least one software function module capable of being stored in the memory 11 in the form of software or firmware or being fixed in the operating system of the genetic testing device 10. The processor 12 can be used to execute the executable modules stored in the memory 11, such as the software functional modules and the computer programs included in the genetic testing control apparatus 300. The gene detection device 10 can cooperate with the execution of the sample nucleic acid purification, the PCR amplification reaction system configuration, the PCR amplification reaction, the PCR fluorescence detection and other automatic operation functions in the gene detection process integrally according to the nucleic acid purification operation progress of the biological sample on the basis of the automatic sample nucleic acid purification operation of the biological sample by the gene detection control device 300, thereby effectively improving the execution efficiency of the whole process of the gene detection from the biological sample to be processed to a specific gene detection result, and realizing the high flux effect and the automatic effect of the gene detection operation.

It is understood that the block diagram shown in FIG. 1 is only two schematic components of the genetic testing device 10, and that the genetic testing device 10 may include more or fewer components than shown in FIG. 1, or may have a different configuration than shown in FIG. 1. The components shown in fig. 1 may be implemented in hardware, software, or a combination thereof.

In the present application, in order to ensure that the gene detection apparatus 10 can perform a rapid automatic nucleic acid purification operation on a single biological sample, or perform a concurrent automatic nucleic acid purification operation on a large number of biological samples, so as to improve the efficiency of nucleic acid purification of the sample, the embodiments of the present application achieve the foregoing object by providing a nucleic acid purification control method. The method for controlling nucleic acid purification provided by the present application is described in detail below.

Referring to fig. 2, fig. 2 is a schematic flow chart of a method for controlling nucleic acid purification according to an embodiment of the present disclosure. In the embodiment of the present application, the method for controlling nucleic acid purification shown in fig. 2 may include steps S210 and S220.

Step S210, obtaining a nucleic acid purification strategy of at least one to-be-processed sample group of the same batch, where each to-be-processed sample group corresponds to one nucleic acid purification strategy, and each to-be-processed sample group includes at least one to-be-processed biological sample.

In this embodiment, for a plurality of to-be-processed biological samples in the same batch, the sample nucleic acid purification operation may be performed on each of the to-be-processed biological samples individually, or the plurality of to-be-processed biological samples in the same batch may be divided into one or more to-be-processed sample groups, so that each to-be-processed sample group includes at least one independent sample, i.e., to-be-processed biological sample, on which the sample nucleic acid purification operation is to be performed. Generally, the nucleic acid purification strategies of a plurality of biological samples to be processed in a sample group to be processed are the same, that is, all biological samples to be processed in the same sample group to be processed adopt the same nucleic acid purification procedure.

In the automatic nucleic acid purification process, a plurality of biological samples to be processed in one sample group to be processed can be transferred to the same octuple tube to be subjected to sample nucleic acid purification operation, so that the nucleic acid purification efficiency of the biological samples to be processed is improved. Correspondingly, the nucleic acid purification strategy corresponding to each sample group to be processed records the name of the reagent, the amount of the reagent, the adding order of the reagent, the specific operation content and the execution sequence corresponding to the sample nucleic acid purification process, and at least one target nucleic acid purification cooperation module required to be used when the operation is executed, wherein the target nucleic acid purification cooperation module is the nucleic acid purification cooperation module 13 participating in the execution of the corresponding sample nucleic acid purification process. Based on different biological samples to be processed or different gene detection types, different sample nucleic acid purification processes exist, and target nucleic acid purification synergistic modules related to the different sample nucleic acid purification processes can be partially identical, completely identical or completely different.

Furthermore, the same sample nucleic acid purification operation process is usually performed on a plurality of biological samples to be processed belonging to the same batch, and after the sample nucleic acid purification operation, the obtained nucleic acid samples are transferred to the same PCR plate for PCR amplification and fluorescence detection, so as to obtain the gene detection result of the biological samples to be processed. Taking a 48-well PCR plate as an example, 48 biological samples to be processed in the same batch are divided into 6 sample groups to be processed, the sample groups are respectively transferred to 6 eight-connecting tubes for sample nucleic acid purification, and the nucleic acid samples obtained by purification are transferred to the 48-well PCR plate for PCR amplification and fluorescence detection so as to obtain gene detection results of the biological samples to be processed. It should be understood that, when the nucleic acid purification strategies performed on a plurality of biological samples to be processed in the same batch are different, the biological samples to be processed that perform the same nucleic acid purification strategy may also be divided into one or more groups of samples to be processed, and the groups are respectively transferred to the eight tubes to perform the corresponding sample nucleic acid purification strategies, and the nucleic acid samples obtained after the sample nucleic acid purification operations are transferred to the same or different PCR plates for PCR amplification and fluorescence detection, so as to obtain the gene detection results of the biological samples to be processed.

Step S220, for each sample group to be processed, according to the respective working states of the multiple nucleic acid purification coordination modules with different functions included in the gene detection device, a target nucleic acid purification coordination module is invoked to sequentially execute each nucleic acid purification process included in the corresponding nucleic acid purification strategy on the sample group to be processed.

In this embodiment, when the gene testing apparatus 10 needs to perform the nucleic acid purification operation on the sample group to be processed of the same batch, according to the respective working states of the multiple nucleic acid purification cooperation modules 13 of the gene testing apparatus 10, one or more nucleic acid purification cooperation modules 13 of the multiple nucleic acid purification cooperation modules 13 are invoked to sequentially perform each nucleic acid purification process in the nucleic acid purification strategy of the sample group to be processed for each sample group to be processed, so as to automatically and rapidly complete the nucleic acid purification operation on the one or more sample groups to be processed of the same batch, thereby performing the concurrent automated nucleic acid purification operation on a large number of biological samples while performing the rapid automated nucleic acid purification operation on a single biological sample, further effectively improving the nucleic acid purification efficiency of the sample, and improving the gene detection efficiency as a whole, the high flux effect and the automation effect of the gene detection operation are realized.

Therefore, by executing the steps S210 and S220, all the nucleic acid purification cooperative modules 13 can perform a rapid automatic nucleic acid purification operation on a single biological sample in a cooperative manner, or perform a concurrent automatic nucleic acid purification operation on a large number of biological samples, so that the nucleic acid purification efficiency of the sample is effectively improved, the gene detection efficiency is integrally improved, and a high-throughput effect and an automation effect of the gene detection operation are realized.

In the present application, in order to ensure that the gene detection apparatus 10 can effectively perform a plurality of corresponding nucleic acid purification processes on at least one sample group to be processed, fully schedule and utilize module hardware resources, and simultaneously avoid process implementation interference with a certain nucleic acid purification process of another sample group to be processed, the present application achieves the foregoing object by effectively controlling the process execution timing of the process to be performed (i.e., the nucleic acid purification process of a certain sample group to be processed that needs to be performed) performed by each nucleic acid purification coordination module 13. The following describes in detail a process execution timing control scheme of the nucleic acid purification coordination module 13 provided in the present application.

Referring to fig. 3, fig. 3 is a flowchart illustrating one of sub-steps included in step S220 in fig. 2. In this embodiment, the step S220 may include a sub-step S221 and a sub-step S222.

And a substep S221, sequentially aiming at each nucleic acid purification process in the nucleic acid purification strategy corresponding to the single sample group to be processed, detecting the current working state of at least one target nucleic acid purification cooperation module executing the nucleic acid purification process.

In this embodiment, each nucleic acid purification cooperative module 13 individually corresponds to a process execution queue, the process execution queue is used for recording to-be-executed nucleic acid purification processes from each to-be-processed sample group that need to be executed by the corresponding nucleic acid purification cooperative module 13, and all the nucleic acid purification cooperative modules 13 execute the to-be-executed nucleic acid purification processes recorded in the process execution queue in sequence according to the process arrangement status in their process execution queues.

The nucleic acid purification processes to be executed recorded in the process execution queue are sorted according to the priority and/or the queue adding time, and when the nucleic acid purification cooperation module 13 works, the added processes close to the top end of the queue in the corresponding process execution queue are executed before the added processes far away from the top end of the queue.

When each nucleic acid purification cooperation module 13 completes the execution of an added process in the corresponding process execution queue, the completed added process is an executed process in the process execution queue, and at this time, the nucleic acid purification cooperation module 13 deletes the executed process in its process execution queue accordingly.

Therefore, the process execution queue of each nucleic acid purification coordination module 13 when the corresponding working state is in the idle state is an empty queue, and the process execution queue of each nucleic acid purification coordination module 13 when the corresponding working state is in the busy state is a non-empty queue. When the gene detection device 10 performs a sample nucleic acid purification operation on a certain sample group to be processed, before each nucleic acid purification process in the nucleic acid purification strategy corresponding to the sample group to be processed is performed, whether the current process execution queues of all target nucleic acid purification coordination modules corresponding to the current nucleic acid purification process to be performed are empty queues or non-empty queues is detected, so as to determine the current working state of the target nucleic acid purification coordination modules corresponding to the current nucleic acid purification process to be performed.

In the sub-step S222, if it is detected that the current working states of all the target nucleic acid purification coordination modules are idle states, all the target nucleic acid purification coordination modules are directly called to cooperatively execute the nucleic acid purification process on the sample group to be processed.

In this embodiment, if it is detected that the current working statuses of all target nucleic acid purification coordination modules corresponding to a certain nucleic acid purification process to be performed are idle statuses, at this time, there is no process implementation interference caused by a certain nucleic acid purification process of other sample groups to be processed, that is, it indicates that the genetic testing apparatus 10 can directly invoke all target nucleic acid purification coordination modules corresponding to the current nucleic acid purification process to be performed to cooperatively perform the nucleic acid purification process, the genetic testing apparatus 10 will directly add the current nucleic acid purification process to be performed in the process execution queue of all target nucleic acid purification coordination modules related to the current nucleic acid purification process to be performed, and invoke all target nucleic acid purification coordination modules related to the current nucleic acid purification process to be performed to cooperatively perform the nucleic acid purification process, thereby effectively avoiding the problem that the control instruction conflicts occur in each nucleic acid purification cooperation module 13 in the gene detection device 10.

Optionally, referring to fig. 4, fig. 4 is a second schematic flowchart of the sub-steps included in step S220 in fig. 2. In this embodiment, the step S220 may further include a substep S223 and a substep S224.

In the substep S223, if it is detected that there is at least one target nucleic acid purification coordination module in which the current working status is busy, the nucleic acid purification process is added to the process execution queue of each of all the target nucleic acid purification coordination modules.

In this embodiment, when there are a plurality of sample groups to be processed to concurrently perform the sample nucleic acid purification operation, one target nucleic acid purification coordination module can only process a certain operation in the nucleic acid purification process corresponding to one sample group to be processed at a certain time, and before the gene detection apparatus 10 performs each nucleic acid purification process in the nucleic acid purification strategy corresponding to each sample group to be processed, it is necessary to detect the current operating state of the target nucleic acid purification coordination module required by the nucleic acid purification process.

If it is detected that the current working state of at least one target nucleic acid purification coordination module in all target nucleic acid purification coordination modules required by a currently to-be-executed nucleic acid purification process of a certain sample group to be processed is busy, it indicates that the target nucleic acid purification coordination module in the busy state is unavailable, for example, a certain target nucleic acid purification coordination module is occupied due to the execution of a certain nucleic acid purification process of another sample group to be processed, and cannot execute the nucleic acid purification process currently required to be executed by a certain sample group to be processed. At this time, the gene detection device 10 adds the currently to-be-executed nucleic acid purification process corresponding to the to-be-processed sample group to the process execution queue of each of all target nucleic acid purification modules required for executing the nucleic acid purification process, so that resources are reasonably allocated when the plurality of to-be-processed sample groups concurrently execute the sample nucleic acid purification operation, and resource conflict is avoided.

In the sub-step S224, when the nucleic acid purification process is at the top of the queue in the process execution queue of each of all target nucleic acid purification modules required for performing the nucleic acid purification process, all target nucleic acid purification modules required for performing the nucleic acid purification process are called to perform the nucleic acid purification process on the sample group to be processed in cooperation with each other.

In this embodiment, after the gene detection apparatus 10 adds the currently performed nucleic acid purification process of each sample group to be processed, which concurrently performs the sample nucleic acid purification operation, to the process execution queue of each respective target nucleic acid purification cooperative module related to the nucleic acid purification process, the gene detection apparatus 10 monitors the process arrangement position of the nucleic acid purification process in the process execution queue of each respective target nucleic acid purification cooperative module in real time, and determines that the nucleic acid purification process can be successfully performed by the target nucleic acid purification cooperative module required for performing the nucleic acid purification process when the nucleic acid purification process is at the top end position of the queue in the process execution queue of each respective target nucleic acid purification cooperative module, and at this time, the gene detection apparatus 10 cooperatively performs the nucleic acid purification process on the corresponding sample group to be processed by calling all target nucleic acid purification cooperative modules related to the nucleic acid purification process correspondingly Therefore, the sample nucleic acid purification operation can be sequentially and concurrently executed by a plurality of sample groups to be processed, and the problem of control instruction conflict of each nucleic acid purification coordination module 13 in the gene detection device 10 can be effectively avoided.

Therefore, by executing the substeps 221 to the substep S224, the rapid automatic nucleic acid purification operation on a single sample group to be processed can be realized, or the process execution time of the process to be executed of a plurality of sample groups to be processed, which is executed concurrently, can be integrally scheduled and effectively controlled, so that the nucleic acid purification processes of the plurality of sample groups to be processed are prevented from interfering or colliding with each other, the process to be executed of each sample group to be processed can be normally executed, and the execution efficiency of the plurality of nucleic acid purification processes can be improved.

Alternatively, in an embodiment of the present embodiment, when a nucleic acid purification process that is not directly performed but currently required to be performed by a certain sample group to be processed is faced by the gene testing apparatus 10, the nucleic acid purification process may be directly added to the end position of the sequence of process execution sequences of all target nucleic acid purification coordination modules required for performing the nucleic acid purification process.

Optionally, in order to further optimize the execution efficiency of the sample nucleic acid purification process, when a plurality of sample groups to be processed concurrently execute respective sample nucleic acid purification processes, the genetic testing apparatus 10 may adjust the ordering of one or more nucleic acid purification processes to be executed in the process execution queue of one or more target nucleic acid purification coordination modules based on an overall optimization or local optimization algorithm, so as to adjust the execution sequence of the current nucleic acid purification processes to be executed in each sample group to be processed, avoid process deadlock, and implement overall scheduling of the nucleic acid purification progress of the plurality of concurrently executed samples to be processed.

Specifically, referring to fig. 5, fig. 5 is a flowchart illustrating the sub-steps included in the sub-step S223 in fig. 4. In another embodiment of this embodiment, the substep S223 may include a substep S2231 and a substep S2232, so as to regulate and control the sequence of the nucleic acid purification processes currently required to be performed by the corresponding genetic testing apparatus 10 for a plurality of groups of samples to be processed that are concurrently performed, so as to ensure that each nucleic acid purification coordination module 13 in the genetic testing apparatus 10 can perform the nucleic acid purification process that is required to be performed in a priority manner in cooperation with each other.

In substep S2231, the execution priority of the nucleic acid purification process is determined based on the completion status of the purification operation and/or the emergency status of the process execution corresponding to the nucleic acid purification process.

In the present embodiment, when the gene assaying device 10 only needs to determine the execution priority of the nucleic acid purification process that corresponds to the nucleic acid purification process that needs to be executed but is not directly executed according to the completion status of the nucleic acid purification operation, the execution priority of the nucleic acid purification process that corresponds to the higher completion status of the nucleic acid purification operation may be set to be higher to ensure that the group of samples to be processed that has the higher completion status of the purification operation can be preferentially completed for all the processes of nucleic acid purification. For example, the ratio of the completed nucleic acid purification processes is determined according to all the nucleic acid purification processes that need to be performed for each sample group to be processed, and the degree of completion of the nucleic acid purification operation for the sample group to be processed is obtained. When the gene assaying device 10 determines the execution priority corresponding to the nucleic acid purification process that needs to be executed but is not directly executed only according to the completion status of the nucleic acid purification operation, the subsequent nucleic acid purification process to be executed of the sample group to be processed, which has a high completion of the nucleic acid purification operation, may be scheduled preferentially, thereby completing preferentially all the nucleic acid purification processes of the sample group to be processed.

When the gene assaying device 10 only needs to determine the execution priority corresponding to the nucleic acid purification process that needs to be executed but is not directly executed according to the emergency situation of the execution of the nucleic acid process, the execution priority of the nucleic acid purification process that is more urgent in the emergency situation of the execution of the process can be set to be higher, so as to ensure that the execution delay of each nucleic acid purification process corresponding to the same sample group to be processed does not occur, and ensure the nucleic acid purification effect of the sample group to be processed. For example, if a biological sample to be processed in a sample group to be processed needs to obtain a gene detection result as soon as possible, the gene testing apparatus 10 may set the sample group to be processed to a high priority level during a nucleic acid purification operation on the sample group to be processed, and preferentially schedule the nucleic acid purification process of the sample group to be processed, so as to ensure that all the nucleic acid purification operations on the sample group to be processed are completed as soon as possible.

When the gene assaying device 10 needs to determine the execution priority corresponding to the nucleic acid purification process that needs to be executed but is not directly executed according to the completion status of the nucleic acid purification operation in cooperation with the process execution emergency, a priority weight may be assigned to each of the completion status of the nucleic acid purification operation and the process execution emergency of the sample group to be processed, so that the execution priority of the nucleic acid purification process of each sample group to be processed is obtained by the priority weighted sum.

In the substep S2232, the nucleic acid purification process is added to the process execution queue of each target nucleic acid purification coordination module, and all the unexecuted processes in the process execution queue of each target nucleic acid purification coordination module are rearranged in priority, wherein the unexecuted processes closer to the top of the queue are higher in execution priority.

Therefore, the present application can control the sequence of the nucleic acid purification processes to be performed by the corresponding gene detection apparatus 10 by performing the sub-step S2231 and the sub-step S2232, so as to ensure that each nucleic acid purification coordination module 13 in the gene detection apparatus 10 can cooperate with each other to preferentially perform the nucleic acid purification processes that are urgently performed.

In the present application, in order to ensure that the gene detection apparatus 10 can effectively improve the execution efficiency of the whole process from the biological sample to be processed to the specific gene detection result on the basis of performing the automated nucleic acid purification operation on the biological sample, and achieve the high-throughput effect and the automation effect of the gene detection operation, the embodiments of the present application achieve the foregoing objects by providing a gene detection control method. The gene detection control method provided in the present application is described in detail below.

Referring to fig. 6, fig. 6 is a schematic flow chart of a gene detection control method according to an embodiment of the present application. In the embodiment of the present application, the gene assaying control method shown in fig. 6 may include steps S410 to S440.

And step S410, calling an amplification reagent adding module included in the gene detection equipment to add PCR amplification reaction reagents on the target PCR plate.

In this embodiment, the amplification reagent adding module 14 in the gene detecting apparatus 10 can add a PCR amplification reaction reagent into the reaction well of the target PCR plate, specifically, according to the nucleic acid purification progress of each to-be-processed sample group, the amplification reagent adding module 14 is called to add the PCR amplification reaction reagent, the whole sample nucleic acid purification operation and the amplification reaction reagent adding operation are scheduled to be executed cooperatively, the effective connection of each functional module is systematically realized in the gene detecting process of the to-be-processed biological sample, and the PCR reaction system construction efficiency is improved.

Step S420, according to the nucleic acid purification control method, a plurality of nucleic acid purification cooperative modules with different functions included in the gene detection device are called to cooperate with each other in a nucleic acid purification process corresponding to at least one sample group to be processed in the same batch, and at least one sample group to be processed after nucleic acid purification is transferred to a target PCR plate, so that nucleic acid samples to be detected are obtained, wherein each sample group to be processed includes at least one biological sample to be processed.

In this embodiment, the specific process of invoking the plurality of nucleic acid purification coordination modules 13 with different functions included in the genetic testing apparatus 10 to execute the respectively matched nucleic acid purification strategies on the one or more sets of samples to be processed of the same batch in coordination with each other may refer to the above detailed description of step S220 and sub-steps S221 to S224, that is, the step of invoking the plurality of nucleic acid purification coordination modules 13 with different functions included in the genetic testing apparatus 10 to execute the respectively matched nucleic acid purification strategies on the at least one set of samples to be processed of the same batch in coordination with each other may include: for each sample group to be processed, according to the respective working states of the multiple nucleic acid purification cooperative modules 13 with different functions included in the gene detection device 10, a target nucleic acid purification cooperative module is invoked on the sample group to be processed to sequentially execute the nucleic acid purification processes included in the corresponding nucleic acid purification strategies, wherein each nucleic acid purification process of the same nucleic acid purification strategy corresponds to at least one target nucleic acid purification cooperative module.

Therefore, when the step S420 is executed, the gene detecting apparatus 10 can be implemented according to the specific content of the step S220 included in the above-mentioned nucleic acid purification control method, so as to transfer the sample nucleic acid obtained by performing nucleic acid purification on at least one sample group to be processed of the same batch onto the target PCR plate added with the PCR amplification reaction reagent, and obtain the currently corresponding nucleic acid sample to be detected. Wherein each nucleic acid sample to be detected individually corresponds to one biological sample to be processed.

Step S430, a sample amplification processing module included in the gene detection device 10 is called, so that all the nucleic acid samples to be detected on the target PCR plate complete the gene amplification reaction under the action of the PCR amplification reaction reagent.

Step S440, a gene fluorescence detection module included in the gene detection device 10 is called to perform fluorescence detection on all to-be-detected nucleic acid samples after gene amplification is completed on the target PCR plate, so as to obtain corresponding sample gene detection results.

Therefore, the present application can further realize the automatic gene detection operation of the biological samples to be processed by executing the steps S410 to S440 on the basis of performing the automatic nucleic acid purification operation on the biological samples to be processed of the same batch, so as to effectively improve the nucleic acid purification efficiency and the gene detection efficiency of the samples, and realize the high flux effect and the automatic effect of the gene detection operation of the samples.

In the present application, in order to ensure that the gene assaying device 10 can execute the above-described nucleic acid purification control method by the nucleic acid purification control apparatus 100, the present application realizes the aforementioned functions by dividing the functional blocks of the nucleic acid purification control apparatus 100. The following describes the specific composition of the nucleic acid purification control apparatus 100 provided in the present application.

Referring to FIG. 7, FIG. 7 is a schematic diagram illustrating the structure of a control apparatus 100 for nucleic acid purification according to an embodiment of the present disclosure. In the embodiment of the present application, the nucleic acid purification control apparatus 100 may include a purification strategy acquisition module 110 and a purification component calling module 120.

A purification strategy obtaining module 110, configured to obtain nucleic acid purification strategies of at least one sample group to be processed of the same batch, where each sample group to be processed includes at least one biological sample to be processed.

A purification component calling module 120, configured to, for each sample group to be processed, call a target nucleic acid purification coordination module on the sample group to be processed according to respective working states of a plurality of nucleic acid purification coordination modules with different functions included in the gene detection device to sequentially perform each nucleic acid purification process included in a corresponding nucleic acid purification strategy, where each nucleic acid purification process of the same nucleic acid purification strategy corresponds to at least one target nucleic acid purification coordination module.

Alternatively, referring to fig. 8, fig. 8 is a schematic diagram illustrating the refinement component invocation module 120 in fig. 7. In the embodiment of the present application, each nucleic acid purification coordination module 13 sequentially executes the added processes of the process execution queue according to the process arrangement status of its own process execution queue, and deletes the executed processes of the process execution queue accordingly, in which case the purification component calling module 120 may include an operating status detection sub-module 121 and a purification process execution sub-module 122.

The working state detection submodule 121 is configured to detect, sequentially for each nucleic acid purification process in the nucleic acid purification strategy corresponding to a single sample group to be processed, a current working state of at least one target nucleic acid purification coordination module that performs the sample nucleic acid purification process.

A purification process execution sub-module 122, configured to, if the working state detection sub-module 121 detects that the current working states of all the target nucleic acid purification cooperative modules are idle states, directly invoke all the target nucleic acid purification cooperative modules to cooperatively execute the nucleic acid purification process on the sample group to be processed, where a process execution queue of each nucleic acid purification cooperative module in the idle state is an empty queue.

In this embodiment, the purification component calling module 120 may further include a purification process loading sub-module 123.

A purification process loading sub-module 123, configured to add the nucleic acid purification process to the respective process execution queues of all the target nucleic acid purification coordination modules if the working status detection sub-module 121 detects that the current working status of at least one target nucleic acid purification coordination module is busy, where the process execution queue of each nucleic acid purification coordination module in the busy status is a non-empty queue.

The purification process execution sub-module 122 is further configured to, in a case that the nucleic acid purification process is at a top position of the sequence in the process execution sequence of each of all the target nucleic acid purification coordination modules, invoke all the target nucleic acid purification coordination modules to cooperatively execute the nucleic acid purification process on the sample group to be processed.

Optionally, referring to fig. 9, fig. 9 is a schematic diagram illustrating the composition of the purification process loading submodule 123 in fig. 8. In this embodiment, the purification process loading sub-module 123 may include a process priority determination unit 1231 and a process addition rearrangement unit 1232.

A process priority determination unit 1231 configured to determine an execution priority of the nucleic acid purification process based on a completion status of the nucleic acid purification operation and/or a process execution emergency status corresponding to the nucleic acid purification process.

A process-adding rearranging unit 1232 for adding the nucleic acid purification process to the process-execution queue of each target nucleic acid purification cooperative module and rearranging the priority of all the unexecuted processes in the process-execution queue of each target nucleic acid purification cooperative module, wherein the unexecuted processes closer to the top position of the queue have higher execution priority.

The basic principle and the technical effects of the nucleic acid purification control apparatus 100 according to the embodiment of the present application are the same as those of the nucleic acid purification control method described above. For a brief description, the contents of the above description of the control method for nucleic acid purification can be referred to where not mentioned in this example section.

In the present application, in order to ensure that the gene testing apparatus 10 can execute the above-mentioned gene testing control method through the gene testing control device 300, the present application implements the aforementioned functions by dividing the function modules of the gene testing control device 300. The specific components of the gene testing control device 300 provided in the present application are described below.

Referring to fig. 10, fig. 10 is a schematic diagram illustrating a configuration of a gene detection control apparatus 300 according to an embodiment of the present application. In the embodiment of the present application, the gene testing control apparatus 300 may include a reagent addition invoking module 310, a nucleic acid purification control apparatus 100, an amplification process invoking module 320, and a gene testing invoking module 330.

And a reagent adding and calling module 310, configured to call an amplification reagent adding module included in the gene detection apparatus to add a PCR amplification reaction reagent on the target PCR plate.

The nucleic acid purification control device 100 is configured to invoke a nucleic acid purification process corresponding to at least one sample group to be processed in the same batch by using a plurality of nucleic acid purification cooperative modules with different functions included in the genetic testing apparatus in a cooperative manner, and transfer at least one sample group to be processed after nucleic acid purification to a target PCR plate to obtain nucleic acid samples to be detected, where each sample group to be processed includes at least one biological sample to be processed.

And the amplification processing calling module 320 is used for calling a sample amplification processing module included in the gene detection equipment to enable all nucleic acid samples to be detected on the target PCR plate to complete gene amplification reaction under the action of the PCR amplification reaction reagent.

And the gene detection calling module 330 is used for calling a gene fluorescence detection module included in the gene detection equipment to perform fluorescence detection on all nucleic acid samples to be detected after gene amplification on the target PCR plate is completed, so as to obtain corresponding sample gene detection results.

The nucleic acid purification control apparatus 100 can correspondingly execute step S220 and substeps S221 to substep S224 of the nucleic acid purification control method, and the specific composition of the nucleic acid purification control apparatus 100 is described in detail in reference to the functional blocks in fig. 7 to 9, which is not repeated herein.

The basic principle and the technical effects of the gene assaying control device 300 according to the embodiment of the present application are the same as those of the gene assaying control method described above. For a brief description, the description of the gene detection control method can be referred to, where not mentioned in this embodiment section.

In the embodiments provided in the present application, it should be understood that the disclosed apparatus and method may be implemented in other ways. The apparatus embodiments described above are merely illustrative, and for example, the flowchart and block diagrams in the figures illustrate the architecture, functionality, and operation of possible implementations of apparatus, methods and computer program products according to embodiments of the present application. In this regard, each block in the flowchart or block diagrams may represent a module, segment, or portion of code, which comprises one or more executable instructions for implementing the specified logical function(s). It should also be noted that, in some alternative implementations, the functions noted in the block may occur out of the order noted in the figures. For example, two blocks shown in succession may, in fact, be executed substantially concurrently, or the blocks may sometimes be executed in the reverse order, depending upon the functionality involved. It will also be noted that each block of the block diagrams and/or flowchart illustration, and combinations of blocks in the block diagrams and/or flowchart illustration, can be implemented by special purpose hardware-based systems which perform the specified functions or acts, or combinations of special purpose hardware and computer instructions.

In addition, functional modules in the embodiments of the present application may be integrated together to form an independent part, or each module may exist separately, or two or more modules may be integrated to form an independent part. The functions, if implemented in the form of software functional modules and sold or used as a stand-alone product, may be stored in a readable storage medium. Based on such understanding, the technical solution of the present application or portions thereof that substantially contribute to the prior art may be embodied in the form of a software product stored in a readable storage medium, which includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method described in the embodiments of the present application. And the aforementioned readable storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, and other various media capable of storing program codes.

In summary, in the nucleic acid purification control method and apparatus, the gene detection device, and the readable storage medium provided by the present application, under the condition that a respective sample nucleic acid purification strategy of at least one to-be-processed sample group including at least one to-be-processed biological sample of the same batch is obtained, for each to-be-processed sample group, in combination with the respective working states of a plurality of nucleic acid purification coordination modules included in the gene detection device, the adapted at least one target nucleic acid purification coordination module is called to sequentially execute each nucleic acid purification process included in the corresponding nucleic acid purification strategy on a single to-be-processed sample group, so that all the nucleic acid purification coordination modules can perform a rapid automatic nucleic acid purification operation on a single biological sample in a mutual coordination manner, or perform a concurrent automatic nucleic acid purification operation on a large number of biological samples, therefore, the purification efficiency of the sample nucleic acid is effectively improved, the gene detection efficiency is integrally improved, and the high-flux effect and the automation effect of the gene detection operation are realized.

The above description is only for various embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily conceive of changes or substitutions within the technical scope of the present application, and all such changes or substitutions are included in the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims

1. A method for controlling purification of a nucleic acid, the method comprising:

2. The method according to claim 1, wherein each nucleic acid purification coordination module executes the added processes of the process execution queue in turn according to the process arrangement status of its own process execution queue, and deletes the executed processes of the process execution queue accordingly;

3. The method according to claim 2, wherein the step of calling the target nucleic acid purification coordination module to sequentially perform the nucleic acid purification processes included in the corresponding nucleic acid purification strategy on the sample group to be processed according to the respective working states of the plurality of nucleic acid purification coordination modules with different functions included in the genetic testing equipment further comprises:

4. The method of claim 3, wherein the step of adding the nucleic acid purification process to the process execution queue of each of the target nucleic acid purification coordination modules comprises:

5. A gene detection control method, comprising:

the nucleic acid purification control method according to any one of claims 1 to 4, wherein a plurality of nucleic acid purification coordination modules with different functions, which are included in the genetic testing equipment, are invoked to cooperatively perform a corresponding nucleic acid purification process on at least one sample group to be processed of the same batch, and the at least one sample group to be processed, which is subjected to nucleic acid purification, is transferred to the target PCR plate, so as to obtain nucleic acid samples to be detected, wherein each sample group to be processed includes at least one biological sample to be processed;

6. A nucleic acid purification control apparatus, characterized in that the apparatus comprises:

7. The apparatus of claim 6, wherein each nucleic acid purification coordination module sequentially executes the added processes of the process execution queue according to the process arrangement status of the process execution queue, and deletes the executed processes of the process execution queue, and the purification component calling module comprises:

8. The apparatus of claim 7, wherein the purification component invocation module further comprises:

9. The apparatus of claim 8, wherein the purification process loading sub-module comprises:

10. A gene testing control device, comprising:

the nucleic acid purification control device of any one of claims 6 to 9, configured to invoke a plurality of nucleic acid purification coordination modules with different functions included in the genetic testing equipment to cooperatively perform a corresponding nucleic acid purification process on at least one sample group to be processed in the same batch, and transfer the at least one sample group to be processed after nucleic acid purification onto the target PCR plate to obtain nucleic acid samples to be detected, where each sample group to be processed includes at least one biological sample to be processed;

11. A gene detection device is characterized by comprising a processor, a memory, an amplification reagent adding module, a sample amplification processing module, a gene fluorescence detection module and a plurality of nucleic acid purification cooperation modules with different functions, wherein the amplification reagent adding module is used for adding PCR amplification reaction reagents to a PCR plate, the sample amplification processing module is used for enabling nucleic acid samples to complete gene amplification reaction under the action of the PCR amplification reaction reagents, the gene fluorescence detection module is used for carrying out fluorescence detection on all the nucleic acid samples after the gene amplification is completed, and the nucleic acid purification cooperation modules are used for carrying out nucleic acid purification operation on biological samples in a mutually matched mode;

the memory stores a computer program executable by the processor, and the processor is capable of executing the computer program to call the plurality of nucleic acid purification coordination modules to implement the nucleic acid purification control method according to any one of claims 1 to 4, or to call the amplification reagent addition module, the sample amplification processing module, the genetic fluorescence detection module, and the plurality of nucleic acid purification coordination modules to implement the genetic detection control method according to claim 5.

12. A readable storage medium on which a computer program is stored, wherein the computer program, when executed by a processor, implements the nucleic acid purification control method according to any one of claims 1 to 4 or the gene detection control method according to claim 5.