CN114853844B - Protein purification refrigeration component collector - Google Patents
Protein purification refrigeration component collector Download PDFInfo
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- CN114853844B CN114853844B CN202210629981.0A CN202210629981A CN114853844B CN 114853844 B CN114853844 B CN 114853844B CN 202210629981 A CN202210629981 A CN 202210629981A CN 114853844 B CN114853844 B CN 114853844B
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- 238000005057 refrigeration Methods 0.000 title claims abstract description 76
- 238000001742 protein purification Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 101
- 238000012360 testing method Methods 0.000 claims abstract description 26
- 238000002347 injection Methods 0.000 claims abstract description 24
- 239000007924 injection Substances 0.000 claims abstract description 24
- 230000007246 mechanism Effects 0.000 claims abstract description 22
- 238000001704 evaporation Methods 0.000 claims description 38
- 230000008020 evaporation Effects 0.000 claims description 32
- 238000007664 blowing Methods 0.000 claims description 30
- 230000001105 regulatory effect Effects 0.000 claims description 27
- 229920000742 Cotton Polymers 0.000 claims description 10
- 238000009434 installation Methods 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000003507 refrigerant Substances 0.000 claims 1
- 238000004811 liquid chromatography Methods 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 description 18
- 108090000623 proteins and genes Proteins 0.000 description 18
- 238000001816 cooling Methods 0.000 description 14
- 230000002209 hydrophobic effect Effects 0.000 description 11
- 238000000746 purification Methods 0.000 description 10
- 239000002585 base Substances 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 238000009833 condensation Methods 0.000 description 5
- 230000005494 condensation Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000012460 protein solution Substances 0.000 description 5
- 239000008346 aqueous phase Substances 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000003957 anion exchange resin Substances 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000005496 tempering Methods 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000000110 cooling liquid Substances 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to the technical field of liquid chromatography and discloses a protein purification refrigeration component collector, which comprises a case, wherein a refrigeration cavity is arranged in the case, a display screen is arranged at the top end of the case, a temperature sensor is arranged in the refrigeration cavity and is electrically connected with the display screen, a refrigeration module is arranged in the refrigeration cavity, a collection frame is arranged in the refrigeration cavity, a plurality of placing grooves for placing test tubes are formed in the collection frame, and a mounting seat is arranged in the case; according to the invention, the temperature in the refrigerating cavity can be monitored in real time through the temperature sensor, the temperature is displayed through the display screen, the temperature adjustment is more convenient, components in the test tube can be rapidly extracted through the collecting injection head, and the condensed water in the refrigerating cavity can be collected through the condensed water collecting mechanism, so that the quantity of the condensed water beside the test tube rack is reduced, the temperature adjustment speed is prevented from being reduced due to the fact that the content of the condensed water is too high, the temperature adjustment speed is high, and the temperature control precision is higher.
Description
Technical Field
The invention relates to the technical field of liquid chromatography, in particular to a protein purification refrigeration component collector.
Background
Protein purification uses the inherent similarity and difference between different proteins, uses the similarity between various proteins to remove the contamination of non-protein substances, and uses the difference between proteins to purify the target protein from other proteins. The size, shape, charge, hydrophobicity, solubility and biological activity of each protein will vary, and these differences can be used to extract the protein from a mixture such as E.coli lysate to obtain recombinant proteins.
Chinese patent CN106432410a discloses a protein separation and purification device, the feed tank is connected with the two-aqueous phase system through a first drainage tube, and the distillation device is connected with the anion exchange resin device through a second drainage tube. Compared with the prior art, the protein separation and purification device provided by the invention has the advantages that the two-aqueous phase system consisting of the charging tank and the high polymer/salt and the anion exchange resin device are arranged, the protein separation and purification steps are separately processed, the two-aqueous phase system consisting of the high polymer/salt is subjected to primary separation and purification through the charging of the charging tank, and then the two-aqueous phase system consisting of the high polymer/salt is subjected to secondary separation and purification through the anion exchange resin device, so that the pollution-free and step-by-step and sectional processing are realized, the effect is ideal, the protein separation is realized through the adjustment of the temperature, the pH value and the like in the earlier stage, the continuous and automatic production of the protein separation and purification is realized, and the separation and purification efficiency is high.
The protein needs to collect the refrigeration component after the purification is finished, the temperature needs to be strictly controlled in the collection process, the temperature needs to be regulated through the refrigeration component when the temperature is controlled, and the component collectors widely used in the market at present do not have a temperature control device, so that the temperature is not convenient to regulate.
Accordingly, there is a need to provide a protein purification refrigeration component collector that addresses the above-described problems.
Disclosure of Invention
The invention aims to provide a protein purification refrigeration component collector which solves the problems in the background technology.
Based on the thought, the invention provides the following technical scheme: the utility model provides a protein purification refrigeration component collector, includes the quick-witted case, the machine incasement is provided with the refrigeration chamber, the top of machine case is provided with the display screen, the refrigeration intracavity is provided with temperature sensor, temperature sensor with display screen electric connection, the refrigeration intracavity is provided with refrigeration module, the refrigeration intracavity is provided with the collection frame, a plurality of standing grooves that are used for placing the test tube have been seted up on the collection frame, the machine incasement is provided with the mount pad, fixed mounting has the collection injection head on the mount pad, the pointed end towards the standing groove setting of collection injection head, the machine incasement is provided with comdenstion water collection mechanism.
As a further scheme of the invention, the protein purification refrigeration component collector also comprises a component collecting valve, a collecting pipe, a singlechip control system arranged on a base platform, an RS232 interface arranged on the singlechip control system, an Ethernet interface, a pulse signal and a power module arranged on a chassis.
As a further scheme of the invention, a supporting seat is fixedly arranged in the case, a Y-axis moving arm is arranged on the side wall of the supporting seat, an X-axis moving arm is arranged on the Y-axis moving arm in a sliding manner, the mounting seat is arranged on the X-axis moving arm in a sliding manner, a Z-axis moving arm is arranged in the case, and the Z-axis moving arm is in transmission connection with the refrigerating frame.
As a further scheme of the invention, the refrigerating module comprises a refrigerating frame, the refrigerating frame is fixedly arranged in a refrigerating cavity, the refrigerating frame is hollow, the collecting frame is arranged in the refrigerating frame, refrigerating pipes are arranged on the refrigerating frame in a communicating manner, and two end parts of the refrigerating pipes are communicated with the refrigerating frame.
As a further scheme of the invention, the condensed water collecting mechanism comprises a water receiving disc, wherein the water receiving disc is fixedly arranged in the case, the water receiving disc is positioned right below the refrigerating frame, a supporting rod is fixedly arranged at the top end of the water receiving disc, a drainage ring is fixedly arranged at the top end of the supporting rod, the drainage ring is arranged in a square frame shape and is in sliding fit with the refrigerating frame, the outer annular wall of the drainage ring is attached to the inner side wall of the refrigerating frame, a strip-shaped groove is formed in the bottom wall of the refrigerating frame, and the supporting rod penetrates through the strip-shaped groove.
As a further scheme of the invention, a temperature regulating box is fixedly arranged in the case, the temperature regulating box is arranged under the water receiving disc, a heat exchange section is arranged on the refrigerating pipe, the heat exchange section is arranged in the temperature regulating box, an evaporation plate is arranged in the temperature regulating box and is rotationally connected with the temperature regulating box through a rotating shaft, a condensation water pipe is arranged at the bottom end of the water receiving disc in a communicating manner, the bottom end of the condensation water pipe stretches into the temperature regulating box, and an air blast mechanism is arranged at one side of the temperature regulating box.
As a further scheme of the invention, the air blowing mechanism comprises an air inlet pipe, an air blowing box and a fan box, wherein the air blowing box is fixedly arranged on the side wall of the temperature regulating box, the air blowing box is communicated with the inner cavity of the temperature regulating box, the fan box is arranged between the air inlet pipe and the air blowing box, the air inlet pipe is communicated with the air blowing box through the fan box, a fan is arranged in the fan box, the side wall of the air blowing box is communicated with an exhaust box, the air blowing box is opposite to the exhaust box, and the side wall of the exhaust box far away from the temperature regulating box is communicated with an exhaust pipe.
As a further scheme of the invention, a plurality of condensate pipes are provided, drainage ropes are arranged in the condensate pipes, a limit groove is formed in the bottom wall of the refrigeration frame, the top ends of the drainage ropes penetrate through the limit groove and are fixedly connected with the bottom wall of the drainage ring, and the bottom ends of the drainage ropes are fixedly connected with one side, far away from the rotating shaft, of the evaporation plate.
As a further scheme of the invention, the top wall of the evaporation plate is provided with a groove, and a plurality of cotton wool are arranged in the groove and vertically arranged on the bottom wall of the groove.
As a further scheme of the invention, a plurality of collecting injection heads are arranged, the collecting injection heads are fixedly arranged on the mounting seat, a plurality of conveying pipes are fixedly arranged on the side wall of the mounting seat far away from the collecting injection heads, and the conveying pipes are respectively communicated with the collecting injection heads.
As a further scheme of the invention, the bottom end of the condensate pipe is provided with a drain hole, and the water outlet end of the drain outlet is arranged towards one side of the evaporation plate, which is close to the rotating shaft.
As a further scheme of the invention, the drainage rope comprises a hydrophobic section and a hydrophilic section, wherein the hydrophobic section is arranged above the hydrophilic section, the joint of the hydrophobic section and the hydrophilic section is arranged in the condensation water pipe, the hydrophobic section is made of a hydrophobic material, and the hydrophilic section is made of a hydrophilic material.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, the cooling module is used for cooling the test tubes in the cooling cavity, the collection frame is used for accurately placing a plurality of test tubes, meanwhile, a plurality of groups of protein solutions are purified, the temperature in the cooling cavity can be monitored in real time through the temperature sensor, the temperature is displayed through the display screen, the temperature adjustment is convenient, the components in the test tubes can be rapidly extracted through the collection injection head, the condensed water in the cooling cavity can be collected through the condensed water collection mechanism, the quantity of condensed water beside the test tube frame is reduced, the slowing of the temperature adjustment speed caused by the overhigh content of the condensed water is avoided, the temperature adjustment speed is high, and the temperature control precision is high.
Drawings
FIG. 1 is a schematic three-dimensional structure of the present invention;
FIG. 2 is a schematic three-dimensional structure of a chassis according to the present invention;
FIG. 3 is a schematic view of a three-dimensional structure of the invention with a chassis removed;
FIG. 4 is a schematic view of a three-dimensional structure of a mounting base and a collection injector of the present invention;
FIG. 5 is a schematic view of a three-dimensional structure of the condensate collection mechanism of the present invention;
FIG. 6 is an enlarged schematic view of the structure of FIG. 5A according to the present invention;
FIG. 7 is a schematic three-dimensional view of the water pan and the temperature regulating box of the present invention;
FIG. 8 is a schematic cross-sectional view of a condensate collection mechanism according to the present invention;
FIG. 9 is an enlarged schematic view of the structure of FIG. 8B according to the present invention;
fig. 10 is an enlarged schematic view of the structure of fig. 8 at C according to the present invention.
In the figure: 1. a chassis; 101. a refrigerating chamber; 2. a display screen; 3. a support base; 4. a Y-axis moving arm; 5. an X-axis moving arm; 6. a mounting base; 7. a refrigeration frame; 701. a limit groove; 8. a collection rack; 9. a water receiving tray; 10. a temperature regulating box; 11. collecting the injection head; 12. a delivery tube; 13. a drainage ring; 14. a refrigeration tube; 1401. a heat exchange section; 15. a support rod; 16. a blower box; 17. a fan case; 18. an air inlet pipe; 19. an exhaust box; 20. an exhaust pipe; 21. a condenser water pipe; 2101. a drain hole; 22. a drainage rope; 23. a rotating shaft; 24. an evaporation plate; 25. cotton wool.
Detailed Description
Referring to fig. 1 to 5, in the embodiment of the invention, a protein purification refrigeration component collector comprises a case 1, wherein a refrigeration cavity 101 is arranged in the case 1, a display screen 2 is arranged at the top end of the case 1, a temperature sensor is arranged in the refrigeration cavity 101 and is electrically connected with the display screen 2, a refrigeration module is arranged in the refrigeration cavity 101, a collection frame 8 is arranged in the refrigeration cavity 101, a plurality of placing grooves for placing test tubes are formed in the collection frame 8, a mounting seat 6 is arranged in the case 1, a collection injection head 11 is fixedly arranged on the mounting seat 6, the tip end of the collection injection head 11 faces the placing grooves, and a condensed water collection mechanism is arranged in the case 1; during the use, place the test tube that is equipped with protein solution in the standing groove on collecting rack 8, close quick-witted case 1 after, cool down to the test tube in the refrigeration chamber 101 through refrigerating module, the redrive mount pad 6 removes, make and collect injection head 11 insert in the test tube, and absorb the protein in the test tube, thereby realize the collection of the protein after the purification, can accurately place a plurality of test tubes through collecting rack 8, carry out purification treatment to multiunit protein solution simultaneously, but temperature in the real-time supervision refrigeration chamber 101 through temperature sensor, and show the temperature through display screen 2, it is comparatively convenient to adjust the temperature, but collect the component in the test tube fast through collecting injection head 11, can collect the comdenstion water in the refrigeration chamber 101 through comdenstion water collection mechanism, the quantity of the side comdenstion water of test tube rack has been reduced, avoid comdenstion water content too high to lead to the tempering speed to become slow, the tempering speed is fast, the control by temperature precision is higher.
In this embodiment, preferably, the invention adopts a single-chip microcomputer control system, and the protein purification refrigeration component collector further comprises a component collecting valve, a collecting pipe, a single-chip microcomputer control system arranged on a base platform, an RS232 interface arranged on the single-chip microcomputer control system, an Ethernet interface, a pulse signal and a power module arranged on the case 1; the power supply module is connected with a 220V power socket, a current power supply channel is arranged on a singlechip control system, the singlechip control system is used for supplying power to the refrigerating module, the temperature of the refrigerating cavity 101 is reduced to 4-8 ℃ after the refrigerating module is powered for 3-4 minutes, the display screen 2 displays the current temperature, a protein purification refrigerating component collector can perform collection work at the moment, the singlechip control system is controlled by an upper computer through an RS232 interface, an X-axis moving arm 5 and a Y-axis moving arm 4,Z are controlled through a remote program, corresponding actions are completed within a definite time period, meanwhile, the upper computer program is used for identifying, the collection valve is controlled to collect corresponding test tubes in a corresponding time period through the X-axis moving arm, the upper computer is matched with different collection volumes at the moment according to different projects, and a pulse signal input interface is used for receiving pulse trigger control signals sent by other chromatographic analysis equipment; after the development cost of hardware is fully considered, a singlechip control mode is adopted, a PLC control mode with high price is abandoned, a 32-bit MCU is used as a control core, and the defects of low processing speed, easy halt, small memory and unreliable data storage of a common 8-bit MCU program are abandoned by applying the high-speed digital signal processing capability, a powerful multitask management function and an embedded control function; the refrigerating cavity 101 adopts the stainless steel arm, the interlayer is provided with the heat insulation surface, the chassis 1 adopts galvanized sheet and spray surface treatment, and the refrigerating cavity is economical and resistant to strong acid and alkali, and has good heat insulation effect; the invention uses a novel controller, has a powerful floating point operation function, accurately calculates the outflow time and outflow volume of liquid, and realizes the collection control of the timing titration amount. The peak-output pulse signals output by other chromatographic analysis equipment are captured rapidly, so that the XYZ three-axis accurate control is realized, the component collector tube moves up and down through the Y axis, the height of the component collector tube entering the inside of the collecting tube is adjusted, and the collecting tube frames with different heights can be met. Through control program, realize automatic rising, the waste liquid pipeline of installation discharges the waste liquid in collection frame 8 base bottom, can fine control waste liquid discharge, and simultaneously at the collecting pipe in the operation, the liquid is unrestrained, has avoided the corruption that causes collection frame 8 and refrigeration chamber 101. The miniature injection head is adopted, so that the collecting liquid is prevented from being stuck to the injection head and splashing; the peltier refrigerating module is adopted, so that the power is low, the refrigerating effect is good, the cooling is fast, and the constant temperature is stable; the inlet collecting frame 8 is adopted, so that the strong acid and alkali resistance effect is good; by adopting the touch type liquid crystal display screen 2, different collection modes and parameter settings can be carried out according to the needs, the running state of the component collector can be monitored better, the temperature can be monitored in real time, and the power module provides power for the whole system.
In this embodiment, preferably, the chassis 1 is fixedly provided with a support seat 3, a Y-axis moving arm 4 is disposed on a side wall of the support seat 3, an X-axis moving arm 5 is slidably disposed on the Y-axis moving arm 4, the mounting seat 6 is slidably mounted on the X-axis moving arm 5, the Y-axis moving arm 4 is used for driving the X-axis moving arm 5 to slide up and down relative to the Y-axis moving arm 4, and the X-axis moving arm 5 is used for driving the mounting seat 6 to slide left and right relative to the X-axis moving arm 5.
In this embodiment, preferably, a Z-axis moving arm is disposed in the chassis 1, and the Z-axis moving arm is in transmission connection with the refrigeration frame 7, and the Z-axis moving arm is used to drive the refrigeration frame 7 and the collection frame 8 to move back and forth relative to the chassis 1, and the X-axis moving arm 5, the Y-axis moving arm 4, and the Z-axis moving arm are all in the prior art, so they are not described in detail.
In this embodiment, preferably, the refrigeration module includes a refrigeration frame 7, the refrigeration frame 7 is fixedly installed in the refrigeration cavity 101, the refrigeration frame 7 is hollow, the collection rack 8 is disposed in the refrigeration frame 7, a refrigeration pipe 14 is disposed on the refrigeration frame 7 in a communicating manner, and two ends of the refrigeration pipe 14 are both communicated with the refrigeration frame 7; the refrigerating frame 7 and the refrigerating pipe 14 are filled with a cooling liquid for transferring heat.
In this embodiment, preferably, the collection injector 11 is provided in plurality, the collection injectors 11 are fixedly mounted on the mounting base 6, the side wall of the mounting base 6 away from the collection injector 11 is fixedly provided with a plurality of conveying pipes 12, and the conveying pipes 12 are respectively communicated with the collection injectors 11; thereby simultaneously collecting the components in a plurality of test tubes.
Referring to fig. 3 to 5, in the embodiment of the present invention, the condensate water collecting mechanism includes a water receiving disc 9, the water receiving disc 9 is fixedly installed in the chassis 1, and the water receiving disc 9 is located under the refrigeration frame 7, a supporting rod 15 is fixedly installed at the top end of the water receiving disc 9, a drainage ring 13 is fixedly installed at the top end of the supporting rod 15, the drainage ring 13 is configured as a square frame, the drainage ring 13 is in sliding fit with the refrigeration frame 7, an outer annular wall of the drainage ring 13 is attached to an inner side wall of the refrigeration frame 7, a strip-shaped groove is formed in a bottom wall of the refrigeration frame 7, and the supporting rod 15 penetrates through the strip-shaped groove; when the inside wall of refrigeration frame 7 produces the comdenstion water, along with refrigeration frame 7 back and forth movement under Z axle motion arm for drainage ring 13 moves relative refrigeration frame 7, and the comdenstion water of refrigeration frame 7 inner wall gathers gradually on drainage ring 13, and flows downwards along the rampart of drainage ring 13, finally falls on water collector 9, thereby realizes the collection to the comdenstion water, and the collection of comdenstion water is gone on by oneself at refrigeration frame 7 removal in-process, does not need other mechanisms to drive, and it is comparatively convenient to use.
In this embodiment, preferably, the temperature adjusting box 10 is fixedly installed in the chassis 1, the temperature adjusting box 10 is disposed under the water pan 9, the heat exchange section 1401 is disposed on the cooling tube 14, the heat exchange section 1401 is disposed in the temperature adjusting box 10, a cooling component is disposed at the bottom of an inner cavity of the temperature adjusting box 10, the cooling component is a cooling plate, and the like, and the heat exchange section 1401 can be cooled by the cooling plate, so that the cooling tube 14 is cooled, and the type of the cooling plate is bs400.
Referring to fig. 5 to 10, in the embodiment of the present invention, an evaporation plate 24 is disposed in the temperature adjustment box 10, the evaporation plate 24 is rotatably connected with the temperature adjustment box 10 through a rotating shaft 23, a condensation water pipe 21 is disposed at the bottom end of the water pan 9 in a communicating manner, the bottom end of the condensation water pipe 21 extends into the temperature adjustment box 10, and a blowing mechanism is disposed at one side of the temperature adjustment box 10; the condensed water entering the water receiving disc 9 flows into the temperature regulating box 10 through the condensed water pipe 21 and falls on the evaporating plate 24, the air is blown into the temperature regulating box 10 through the air blowing mechanism, so that the water on the evaporating plate 24 is evaporated, the heat is absorbed during evaporation of the condensed water, the temperature in the temperature regulating box 10 is reduced, and the heat exchange section 1401 of the refrigerating pipe 14 is arranged in the temperature regulating box 10, so that the refrigerating pipe 14 is cooled better, and the utilization of the condensed water is realized.
In this embodiment, preferably, the air blowing mechanism includes an air inlet pipe 18, an air blowing box 16 and a fan box 17, the air blowing box 16 is fixedly installed on a side wall of the temperature regulating box 10, the air blowing box 16 is communicated with an inner cavity of the temperature regulating box 10, the fan box 17 is disposed between the air inlet pipe 18 and the air blowing box 16, the air inlet pipe 18 and the air blowing box 16 are communicated through the fan box 17, a fan is disposed in the fan box 17, an exhaust box 19 is disposed on a side wall of the air blowing box 16 in a communicating manner, the air blowing box 16 is disposed opposite to the exhaust box 19, and an exhaust pipe 20 is disposed on a side wall, far from the temperature regulating box 10, of the exhaust box 19 in a communicating manner; the fan can be started to blow air into the blowing box 16, and the blown air evaporates the condensed water and then is discharged through the exhaust box 19 and the exhaust pipe 20, so that the excessive water vapor content in the case 1 is avoided.
In this embodiment, preferably, a plurality of condensate pipes 21 are provided, a plurality of drain ropes 22 are disposed in the condensate pipes 21, a limiting groove 701 is formed in the bottom wall of the refrigeration frame 7, the top end of each drain rope 22 passes through the limiting groove 701 and is fixedly connected with the bottom wall of the corresponding drain ring 13, and the bottom end of each drain rope 22 is fixedly connected with one side, far away from the rotating shaft 23, of the evaporation plate 24; when the refrigeration frame 7 moves, the limiting groove 701 on the refrigeration frame 7 pushes the drainage rope 22 to move, so that the bottom end of the drainage rope 22 moves upwards, the evaporation plate 24 is driven to rotate upwards, when the refrigeration frame 7 moves reversely, the bottom end of the drainage rope 22 moves downwards, so that the evaporation plate 24 rotates downwards under the action of self gravity, water entering the water receiving disc 9 flows into the condensate pipe 21, water in the condensate pipe 21 is led out in the process of moving the drainage rope 22 up and down, the water outlet speed of the condensate pipe 21 is controlled, and the phenomenon that the evaporation plate 24 cannot evaporate due to too high drainage speed of the condensate pipe 21 is avoided.
In this embodiment, preferably, a groove is formed on the top wall of the evaporation plate 24, and a plurality of cotton fibers 25 are disposed in the groove, and the cotton fibers 25 stand on the bottom wall of the groove; through the setting of cotton fibre 25 for can have more water to adhere to on cotton fibre 25, make the area of contact increase of water and wind, thereby improve the evaporation rate of water, and at evaporating plate 24 upper and lower pivoted in-process, cotton fibre 25 can last the swing, make cotton fibre 25 by the difficult subsides on the diapire of recess behind the water wetting.
In this embodiment, preferably, the bottom end of the condensate pipe 21 is provided with a drain hole, the water outlet end of the drain hole is disposed towards one side of the evaporation plate 24 near the rotating shaft 23, when the condensate water generating speed is faster, the drain hole can be used for draining water, in the process of rotating the evaporation plate 24, on one hand, the position of the condensate water drops on the evaporation plate 24 continuously changes, so that the condensate water is rapidly and uniformly distributed on the evaporation plate 24, on the other hand, the included angle between the wind direction of the wind blown by the blower box 16 and the evaporation plate 24 continuously changes, so that the wind energy is blown on the evaporation plate 24 from multiple angles, the contact area between the water drops on the evaporation plate 24 and the wind is increased, and the evaporation speed of the water drops is increased.
In this embodiment, preferably, the drainage rope 22 includes a hydrophobic section and a hydrophilic section, the hydrophobic section is disposed above the hydrophilic section, the junction of the hydrophobic section and the hydrophilic section is disposed in the condenser water pipe 21, the hydrophobic section is made of a hydrophobic material, the hydrophilic section is made of a hydrophilic material, the hydrophobic section can avoid excessive water to be adsorbed at the top of the drainage rope 22, the evaporation efficiency is affected, and the hydrophilic end can enable more water to be adsorbed at the bottom of the drainage rope 22, so that evaporation of water can be performed when wind blows on the drainage rope 22, and the evaporation efficiency of water is improved.
The working principle of the invention is as follows: placing test tubes filled with protein solution in a placing groove on a collecting rack 8, closing a machine case 1, cooling the test tubes in a refrigerating cavity 101 through a refrigerating module, driving a mounting seat 6 to move, enabling a collecting injection head 11 to be inserted into the test tubes, absorbing proteins in the test tubes, collecting purified proteins, accurately placing a plurality of test tubes through the collecting rack 8, purifying a plurality of groups of protein solution, monitoring the temperature in the refrigerating cavity 101 in real time through a temperature sensor, displaying the temperature through a display screen 2, adjusting the temperature conveniently, rapidly extracting components in the test tubes through the collecting injection head 11, collecting condensed water in the refrigerating cavity 101 through a condensed water collecting mechanism, reducing the quantity of condensed water beside the test tube rack, avoiding slowing down the temperature adjustment speed due to overhigh condensed water content, and having high temperature adjustment speed and high temperature control precision; when the inner side wall of the refrigeration frame 7 generates condensed water, the drainage ring 13 moves relative to the refrigeration frame 7 along with the front-back movement of the refrigeration frame 7 under the Z-axis movement arm, and in the moving process of the refrigeration frame 7, the condensed water on the inner wall of the refrigeration frame 7 gradually gathers on the drainage ring 13 and flows downwards along the annular wall of the drainage ring 13, and finally falls on the water receiving disc 9, so that the condensed water is collected; the condensed water entering the water receiving disc 9 flows into the temperature regulating box 10 through the condensed water pipe 21 and falls on the evaporating plate 24, the air is blown into the temperature regulating box 10 through the air blowing mechanism, so that the water on the evaporating plate 24 is evaporated, the condensed water absorbs heat during evaporation, the temperature in the temperature regulating box 10 is reduced, and the heat exchange section 1401 of the refrigerating pipe 14 is arranged in the temperature regulating box 10, so that the refrigerating pipe 14 is cooled better; when the refrigeration frame 7 moves, the limiting groove 701 on the refrigeration frame 7 pushes the drainage rope 22 to move, so that the bottom end of the drainage rope 22 moves upwards, the evaporation plate 24 is driven to rotate upwards, when the refrigeration frame 7 moves reversely, the bottom end of the drainage rope 22 moves downwards, so that the evaporation plate 24 rotates downwards under the action of self gravity, water entering the water receiving disc 9 flows into the condensate pipe 21, water in the condensate pipe 21 is led out in the process of moving the drainage rope 22 up and down, the water outlet speed of the condensate pipe 21 is controlled, and the phenomenon that the evaporation plate 24 cannot evaporate due to too high drainage speed of the condensate pipe 21 is avoided.
Claims (5)
1. The utility model provides a protein purification refrigeration component collector, includes the quick-witted case, the machine incasement is provided with the refrigeration chamber, the top of machine case is provided with the display screen, the refrigeration intracavity is provided with temperature sensor, temperature sensor with display screen electric connection, its characterized in that: the refrigerating chamber is internally provided with a refrigerating module, the refrigerating chamber is internally provided with a collecting frame, the collecting frame is provided with a plurality of placing grooves for placing test tubes, the machine case is internally provided with a mounting seat, the mounting seat is fixedly provided with a collecting injection head, the tip of the collecting injection head is arranged towards the placing grooves, and the machine case is internally provided with a condensed water collecting mechanism;
the refrigerating module comprises a refrigerating frame, the refrigerating frame is fixedly arranged in a refrigerating cavity, the refrigerating frame is hollow, the collecting frame is arranged in the refrigerating frame, refrigerating pipes are arranged on the refrigerating frame in a communicating mode, and two end portions of the refrigerating pipes are communicated with the refrigerating frame;
a supporting seat is fixedly installed in the case, a Y-axis moving arm is arranged on the side wall of the supporting seat, an X-axis moving arm is arranged on the Y-axis moving arm in a sliding mode, the installation seat is installed on the X-axis moving arm in a sliding mode, a Z-axis moving arm is arranged in the case, and the Z-axis moving arm is in transmission connection with the refrigeration frame;
the condensed water collecting mechanism comprises a water receiving disc, the water receiving disc is fixedly arranged in the case, the water receiving disc is positioned right below the refrigerating frame, a supporting rod is fixedly arranged at the top end of the water receiving disc, a drainage ring is fixedly arranged at the top end of the supporting rod, the drainage ring is in sliding fit with the refrigerating frame, the outer annular wall of the drainage ring is attached to the inner side wall of the refrigerating frame, a strip-shaped groove is formed in the bottom wall of the refrigerating frame, and the supporting rod penetrates through the strip-shaped groove;
the refrigerator comprises a refrigerator body, a water receiving disc, a refrigerating pipe, an evaporation plate, a temperature adjusting box, a condensing water pipe and an air blowing mechanism, wherein the temperature adjusting box is fixedly installed in the refrigerator body and is arranged right below the water receiving disc, the refrigerating pipe is provided with a heat exchange section, the heat exchange section is arranged in the temperature adjusting box, the evaporation plate is arranged in the temperature adjusting box and is rotationally connected with the temperature adjusting box through a rotating shaft, the bottom end of the water receiving disc is communicated with the condensing water pipe, the bottom end of the condensing water pipe extends into the temperature adjusting box, and one side of the temperature adjusting box is provided with the air blowing mechanism;
the collecting injection heads are provided with a plurality of collecting injection heads, the collecting injection heads are fixedly installed on the installation base, a plurality of conveying pipes are fixedly installed on the installation base, far away from the side wall of the collecting injection heads, and the conveying pipes are respectively communicated with the collecting injection heads.
2. A protein purification refrigeration component collector according to claim 1, wherein: the protein purification refrigeration component collector also comprises a component collecting valve, a collecting pipe, a singlechip control system arranged on the base platform, an RS232 interface arranged on the singlechip control system, an Ethernet interface, a pulse signal and a power module arranged on the chassis.
3. A protein purification refrigeration component collector according to claim 1, wherein: the air blowing mechanism comprises an air inlet pipe, an air blowing box and a fan box, wherein the air blowing box is fixedly arranged on the side wall of the temperature regulating box, the air blowing box is communicated with the inner cavity of the temperature regulating box, the fan box is arranged between the air inlet pipe and the air blowing box, the air inlet pipe is communicated with the air blowing box through the fan box, a fan is arranged in the fan box, an exhaust box is arranged on the side wall of the air blowing box in a communicated mode, the air blowing box is opposite to the exhaust box, and an exhaust pipe is arranged on the side wall of the exhaust box, far away from the temperature regulating box, in a communicated mode.
4. A protein purification refrigeration component collector according to claim 1, wherein: the condenser water pipe is provided with a plurality of, and is a plurality of all be provided with the drainage rope in the condenser water pipe, the spacing groove has been seted up to the diapire of refrigeration frame, the top of drainage rope pass the spacing groove with the diapire fixed connection of drainage ring, the bottom of drainage rope with one side fixed connection that the pivot was kept away from to the evaporating plate.
5. The protein purification refrigerant component collector as defined in claim 4, wherein: the top wall of the evaporation plate is provided with a groove, a plurality of cotton wool are arranged in the groove, and the cotton wool is vertically arranged on the bottom wall of the groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210629981.0A CN114853844B (en) | 2022-06-06 | 2022-06-06 | Protein purification refrigeration component collector |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210629981.0A CN114853844B (en) | 2022-06-06 | 2022-06-06 | Protein purification refrigeration component collector |
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| CN114853844A CN114853844A (en) | 2022-08-05 |
| CN114853844B true CN114853844B (en) | 2023-10-27 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004197979A (en) * | 2002-12-16 | 2004-07-15 | Hoshizaki Electric Co Ltd | Freezing device |
| CN204502467U (en) * | 2014-12-31 | 2015-07-29 | 江苏汉邦科技有限公司 | Full-automatic machine mechanical arm fraction collector |
| CN106432410A (en) * | 2016-11-29 | 2017-02-22 | 东北农业大学 | Protein separation and purification device and protein separation and purification method |
| CN111748465A (en) * | 2020-07-20 | 2020-10-09 | 宋文海 | Condensate water collection device for biological cultivation |
-
2022
- 2022-06-06 CN CN202210629981.0A patent/CN114853844B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004197979A (en) * | 2002-12-16 | 2004-07-15 | Hoshizaki Electric Co Ltd | Freezing device |
| CN204502467U (en) * | 2014-12-31 | 2015-07-29 | 江苏汉邦科技有限公司 | Full-automatic machine mechanical arm fraction collector |
| CN106432410A (en) * | 2016-11-29 | 2017-02-22 | 东北农业大学 | Protein separation and purification device and protein separation and purification method |
| CN111748465A (en) * | 2020-07-20 | 2020-10-09 | 宋文海 | Condensate water collection device for biological cultivation |
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| CN114853844A (en) | 2022-08-05 |
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