CN114847227A - Construction method and application of sheep chronic cold stress model - Google Patents
Construction method and application of sheep chronic cold stress model Download PDFInfo
- Publication number
- CN114847227A CN114847227A CN202210591793.3A CN202210591793A CN114847227A CN 114847227 A CN114847227 A CN 114847227A CN 202210591793 A CN202210591793 A CN 202210591793A CN 114847227 A CN114847227 A CN 114847227A
- Authority
- CN
- China
- Prior art keywords
- temperature
- sheep
- cold stress
- days
- construction method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001494479 Pecora Species 0.000 title claims abstract description 96
- 230000008645 cold stress Effects 0.000 title claims abstract description 69
- 230000001684 chronic effect Effects 0.000 title claims abstract description 57
- 238000010276 construction Methods 0.000 title claims abstract description 24
- 238000001816 cooling Methods 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 15
- 230000009467 reduction Effects 0.000 claims description 5
- 230000033228 biological regulation Effects 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 229940079593 drug Drugs 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 230000006389 acute stress response Effects 0.000 claims description 2
- 230000003938 response to stress Effects 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 238000003745 diagnosis Methods 0.000 claims 1
- 230000000384 rearing effect Effects 0.000 claims 1
- 230000008859 change Effects 0.000 abstract description 16
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 238000010171 animal model Methods 0.000 abstract description 6
- 230000001932 seasonal effect Effects 0.000 abstract description 6
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 8
- 230000037396 body weight Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 5
- 235000019687 Lamb Nutrition 0.000 description 4
- 230000002354 daily effect Effects 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000035882 stress Effects 0.000 description 4
- 230000035924 thermogenesis Effects 0.000 description 4
- 206010044565 Tremor Diseases 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 208000001034 Frostbite Diseases 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000009529 body temperature measurement Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000002027 skeletal muscle Anatomy 0.000 description 2
- 230000002277 temperature effect Effects 0.000 description 2
- 238000004861 thermometry Methods 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 208000009084 Cold Injury Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002595 cold damage Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a construction method and application of a sheep chronic cold stress model, and relates to the technical field of animal model construction. It includes: in a feeding room for feeding sheep to be treated, the temperature in the feeding room is continuously and gradiently cooled to-35 ℃ to-20 ℃, and the temperature is kept for 20-25 days at constant temperature. The construction method of the sheep chronic cold stress model provided by the invention is not influenced by day and night temperature difference and seasonal change of the nutritive value of pasture, and can accurately control the temperature at any time, so that the sheep chronic cold stress model with higher quality is provided, and an animal model basis is provided for the follow-up study of the cold stress reaction behavior of sheep.
Description
Technical Field
The invention relates to the technical field of animal model construction, in particular to a construction method and application of a sheep chronic cold stress model.
Background
Cold stress is one of the important factors that restrict livestock production. In northern areas of China, pasture is cold and long in winter, the growing season of pasture is short, and low temperature in winter often induces a series of stress reactions (such as diseases like dyspepsia and diarrhea) to livestock, so that the production performance of the livestock is reduced, and an extreme low temperature event (snow disaster) even leads to massive freezing and death of the livestock. Researchers have found that for every 1 ℃ reduction in temperature, the maintenance requirement for a sheep weighing 35kg will increase by 3.6MJ/kg for ambient temperatures between-18 ℃ and 0 ℃ 0.75 . The temperature is reduced by 1 ℃ every time, and the weight of the sheep loses 5.0-6.0 g every day. It is also found that under the cold stress of-5 ℃, the energy consumption of 40.1kJ is increased for each 1g of the body weight of the Aletan sheep, and the energy consumption of 190.5kJ is increased for each 1g of the body weight of the Hu sheep.
At present, the research on the cold stress reaction of sheep is an important subject by constructing a cold stress model of sheep. However, many sheep cold stress models are derived from a seasonal temperature-changing method, and the sheep winter cold stress model constructed based on natural environment change is strong in operability and simple in management mode. However, the temperature control is not accurate enough, the temperature is in continuous fluctuation in the daytime during the test period, the temperature difference between the highest temperature and the lowest temperature in the daytime is large, and meanwhile, the influence of seasonal change of the nutrient value of the pasture on a cold stress model is ignored, so that the cold stress behavior of the sheep is not objective enough.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a construction method and application of a sheep chronic cold stress model, and provides a more standardized and faster construction method of the sheep chronic cold stress model through accurate temperature control, temperature reduction and constant temperature time regulation.
The invention is realized by the following steps:
the invention provides a construction method of a sheep chronic cold stress model, which comprises the following steps: in a feeding room for feeding sheep to be treated, the temperature in the feeding room is continuously and gradiently cooled to-35 ℃ to-20 ℃, and the temperature is kept for 20-25 days at constant temperature.
The construction method of the sheep chronic cold stress model provided by the invention is not influenced by day and night temperature difference and seasonal change of the nutritive value of pasture, and can accurately control the temperature at any time, so that the sheep chronic cold stress model with higher quality is provided, and an animal model basis is provided for the follow-up study of the cold stress reaction behavior of sheep.
The rapid temperature drop can be avoided by continuous gradient cooling to cause adverse temperature effects, including but not limited to sheep: cold tremor, frostbite, and freeze death. By adopting the continuous gradient cooling, the unexpected influence caused by irregular change of steady step can be avoided.
In the preferred embodiment of the present invention, the continuous gradient cooling is to reduce the temperature once every two days, wherein the temperature is reduced by 4-6 ℃ every time. Control interval two days is cooled down and is adjusted, can prevent on the one hand that the cooling time is too short, leads to the unable adaptive temperature of sheep, causes bad influence, including being not limited to causing the sheep: trembling, cold injury, freeze death, etc.; on the other hand, a corresponding animal model can be obtained in a shorter time, and the time for constructing the model is shortened.
The inventor finds that precise temperature control, final temperature range and continuous gradient change mode are main factors for inducing sheep chronic cold stress.
In an alternative embodiment, the temperature is lowered by 4 ℃, 4.5 ℃, 5 ℃, 5.5 ℃ or 6 ℃ each time.
In the preferred embodiment of the present invention, the temperature is lowered by 5 ℃ each time.
In the preferred embodiment of the present invention, the initial temperature of the feeding chamber is controlled to 20 ℃. + -. 0.5 ℃.
In the preferred embodiment of the invention, the initial temperature in the feeding chamber is set to be 20 ℃, the temperature is reduced by 5 ℃ every two days, and the constant temperature of-20 ℃ is kept for 23-25 days. Under the temperature control condition, the temperature can be controlled more accurately, adverse reaction caused by rapid cooling is avoided, and influence caused by temperature fluctuation and large temperature difference in the daytime under the natural environment condition is also avoided. The method provided by the invention accurately and objectively constructs the sheep chronic cold stress model and provides an effective way for the follow-up deep study of the sheep cold stress reaction.
In the preferred embodiment of the present invention, the temperature is maintained at-20 ℃ for 24 days.
In the following embodiment, the sheep constant-temperature cold room adopts a continuous gradient cooling mode (5 ℃ per 2 days on average), the specific temperature change is 20 ℃,15 ℃,10 ℃, 5 ℃,0 ℃, 5 ℃,10 ℃,15 ℃ and 20 ℃, the temperature is adjusted at 9 ℃ in the morning every time, the target temperature is reached within 30min, the time interval of temperature adjustment is 2 days every time, and finally the temperature is maintained at-20 ℃ for 24 days, and the whole test period is 41 days.
In a preferred embodiment of the present invention, the temperature in the feeding chamber is reduced to the target temperature within 20-30min during continuous gradient cooling.
Cooling and adjusting at 9-10 am; in the preferred embodiment of the present invention, the temperature reduction is adjusted at 9 am.
It should be noted that, as long as the sheep underwent a decrease in body weight (including but not limited to a case where the daily gain was negative) compared to the weight before treatment, the model construction was considered successful. Furthermore, a significant increase in heart rate in the sheep of the treated group was also considered successful in model construction, and chronic cold stress sheep with lower tissue temperatures (longidorsi and gastrocnemius) and rectal temperatures than the sheep of the non-treated group. The lower temperature indicates that the metabolic thermogenesis process of the sheep is not enough to maintain the same temperature as that of the sheep in the control group, and the sheep are in stress reaction. These results demonstrate that continuous gradient cooling successfully induces a sheep model of chronic cold stress.
The invention also provides an application of the sheep chronic cold stress model constructed by the construction method in sheep chronic cold stress reaction research, and the research aims at diagnosing or treating non-diseases.
The invention also provides application of the sheep chronic cold stress model constructed by the construction method in screening sheep anti-chronic stress response medicines or acute stress response medicines.
The invention has the following beneficial effects:
the construction method of the sheep chronic cold stress model provided by the invention is not influenced by day and night temperature difference and seasonal change of the nutritive value of pasture, and can accurately control the temperature at any time, so that the sheep chronic cold stress model with higher quality is provided, and an animal model basis is provided for the follow-up study of the cold stress reaction behavior of sheep.
The inventors can avoid the adverse temperature effects caused by sharp temperature drops by continuous gradient cooling, including but not limited to sheep: cold tremor, frostbite, and freeze death. Compared with a winter sheep cold stress model induced by seasonal temperature change, the method can accurately control the temperature condition of the sheep under the chronic cold stress, avoids the influence caused by temperature fluctuation and large temperature difference in the daytime under the natural environment condition, and lays a foundation condition for deeply researching the behavior change, the physiological characteristics and the molecular regulation of the sheep under the chronic cold stress.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a diagram of continuous gradient cooling in a constant temperature cold chamber of a sheep;
FIG. 2 is a graph of the difference in body weight, daily gain and heart rate between chronic cold stress sheep and control groups;
FIG. 3 is a graph of statistical difference in rectal temperature between chronic cold stress sheep and a control group;
FIG. 4 is a graph of statistical results of the difference in temperature between the longissimus dorsi of a chronic cold stress sheep and a control group;
FIG. 5 is a graph showing statistical results of differences in gastrocnemius temperature between a chronic cold-stressed sheep and a control group.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The embodiment provides a method for constructing a sheep chronic cold stress model, which comprises the following steps:
(1) selecting a research object and designing a test;
selecting 24 Hu sheep with age of 4-5 months, average initial weight of 35.0 +/-0.52 kg and good health condition; the average group was divided into 2 groups, including a control group (constant temperature 20 ℃ C., maintained for 41 days) and a chronic cold stress group (including 17 days of continuous gradient cooling and 24 days as a formal test period), and the test period was 41 in total.
(2) Establishing a sheep chronic cold stress model.
The sheep constant-temperature cold room adopts a continuous gradient cooling mode (5 ℃ is reduced every 2 days on average), the specific temperature change is 20 ℃,15 ℃,10 ℃, 5 ℃,0 ℃, 5 ℃,10 ℃,15 ℃ and 20 ℃, the temperature is adjusted at 9 am every time, the target temperature is reached within 30min, the time interval of temperature adjustment is 2 days every time, and finally the temperature is continuously maintained for 24 days at 20 ℃ below zero.
(3) And (4) detecting the temperature of the sheep tissue.
Changes in temperature of the longissimus dorsi and gastrocnemius muscles of the sheep were continuously monitored on days 40, 41 of the experimental period at 10 time points: 8:00,9:00,10:00,11:00,12:00,13:00,14:00,15:00,16,00,17:00.
Example 2
Compared with example 1, the difference is only in step (2), and the rest of the steps are the same.
And (2) establishing a sheep chronic cold stress model.
The sheep constant-temperature cold room adopts a continuous gradient cooling mode (the temperature is reduced by 4 ℃ every 2 days on average), the specific temperature change is 20 ℃,16 ℃,12 ℃, 8 ℃, 4 ℃,0 ℃, 4 ℃, 8 ℃,12 ℃,16 ℃ and 20 ℃, the temperature is adjusted at 9 am every time, the target temperature is reached within 30min, the time interval of adjusting the temperature is 2 days every time, and finally the temperature is continuously maintained for 24 days at 20 ℃ below zero.
Example 3
Compared with example 1, the difference is only in step (2), and the rest of the steps are the same.
And (2) establishing a sheep chronic cold stress model.
The sheep constant-temperature cold room adopts a continuous gradient cooling mode (the temperature is reduced by 6 ℃ every 2 days on average), the specific temperature change is 25 ℃, 19 ℃,13 ℃, 7 ℃,1 ℃, 4 ℃ below zero, 10 ℃ below zero, 16 ℃ below zero and 20 ℃ below zero, the temperature is adjusted at 9 am every time, the target temperature is reached within 30min, the time interval of adjusting the temperature is 2 days every time, and finally the temperature is continuously maintained at 20 ℃ below zero for 24 days.
Experimental example 1
Sheep tissues, longissimus dorsi (back), gastrocnemius (hind leg), implanted implantable temperature chip thermolchip (SureFlap, usa) were selected before the start of the experiment. Then, the patient was wiped with iodophor to avoid infection.
From the first day of the test, the sheep was subjected to a continuous gradient cooling in a constant temperature cold room according to the method of example 1, with the temperature change being 20 ℃,15 ℃,10 ℃, 5 ℃,0 ℃, 5 ℃,10 ℃,15 ℃ and 20 ℃, and the temperature was adjusted at 9 am each time to reach the target temperature within 30min, with the time interval between each adjustment of the temperature being 2 days, and finally maintained at-20 ℃ for 24 days. The control group was normally fed at room temperature (20 ℃), fed and managed as in example 1. The continuous gradient cool down procedure is illustrated with reference to fig. 1.
After construction, each lamb was measured for changes in sheep body weight and heart rate on day 38 of the full term.
TABLE 1 weight and Heart Rate Difference in sheep from Chronic Cold stress and control groups
Compared with the control group, the weight of the chronic cold stress group is obviously reduced (the initial weight is 35kg), and the average daily gain (-12.55 +/-20.483 g/d) is obviously lower than that of the control group (48.84 +/-15.113 g/d) (Table 1, A-B in figure 2). While there was a significant increase in body weight and heart rate in the chronic cold stress group (table 1, shown as C in figure 2). The weight reduction and the heart rate increase of the sheep subjected to continuous gradient cooling indicate that the cold stress reaction of the sheep is induced, the maintenance demand of the chronic cold stress sheep is increased, and the heart rate increase may be related to the increase of oxygen absorption and consumption of a group calling of the chronic cold stress.
After construction, rectal temperature was measured on day 39 of the experimental period for each lamb.
Specifically, when an electronic anal thermometer (ohm dragon MC-347, ohm dragon health care (China) Limited, Liaoning) is used for measuring temperature, the anal thermometer is thoroughly disinfected by iodine, and then is coated with a lubricant; the root of the tail of the animal is lifted by one hand of a temperature measurer and pushed to the side, and the other hand-held thermometer is slowly inserted into the anus of the sheep for about 3-5 cm; the reading was maintained for 10 seconds and recorded, and 3 consecutive measurements were averaged. After the test, the test paper is wiped clean by using the disinfectant iodine tincture for thorough disinfection for reuse.
TABLE 2 rectal temperature differences between sheep in the Chronic Cold stress group and the control group
Compared with the control group, the rectal temperature of the chronic cold stress group is lower than that of the control group at 6 time points of continuous temperature measurement. The mean value of the chronic cold stress group was significantly lower than that of the control group by 0.28 ℃ (table 2). In particular, the rectal temperature of the sheep in the chronic cold stress group was significantly lower than that in the control group at 0.50 ℃, 0.53 ℃, 0.20 ℃ and the maximum temperature difference was 0.53 ℃ at 3 time points (9:00,10:00,11:00) of continuous temperature measurement (table 2, fig. 3). The continuous gradient cooling causes the rectal temperature of the sheep to be reduced, but the rectal temperature of the sheep in the chronic cold stress group is in a normal range (38-40 ℃), which shows that although the heart rate is increased to possibly represent the increase of respiratory oxygen consumption and the enhancement of metabolic thermogenesis, the enhanced metabolic thermogenesis process is still insufficient to maintain the rectal temperature equal to that of the sheep in the control group, and the sheep are in stress reaction.
After construction, each lamb was measured for different tissue temperatures on days 40 and 41 of the experimental period.
Specifically, each day 8:00 a morning, using a reader BioReader (SureFlap, usa) fitted with an implanted temperature chip to press close to the longissimus dorsi (back) and gastrocnemius (hind legs) of each lamb, scan with gentle movement, perform chip identification and temperature reading. The time of temperature reading record is: 8:00,9:00,10:00,11:00,12:00,13:00,14:00,15:00,16,00,17:00.
TABLE 3 temperature difference between longissimus dorsi of sheep in chronic cold stress group and control group
Compared with the control group, the temperature of the longissimus dorsi muscle of the chronic cold stress group was lower than that of the control group at 10 time points of continuous thermometry (table 3, fig. 4). The average dorsomus longissimus temperature of the chronic cold stress group was 0.32 ℃ lower than that of the control group (table 3). In particular, the rectal temperature of the sheep in the chronic cold stress group is significantly lower than that of the control group by 0.75 ℃ at 8:00, and the temperature difference is maximum at the moment. In addition, the temperature of the 9:00 and 10:00 chronic cold stress group sheep was lower than that of the control group by 0.61 and 0.53 respectively, although there was no statistically significant difference, which may be caused by measurement errors.
TABLE 4 temperature Change in gastrocnemius muscles of sheep in Chronic Cold-stressed and control groups
The gastrocnemius temperature was lower in the chronic cold stress group than in the control group at 10 time points of continuous thermometry (except 14:00) compared to the control group (table 4, fig. 5). The average gastrocnemius temperature in the chronic cold stress group was 0.29 ℃ lower than that in the control group, and there was a tendency to decrease (Table 4). In particular, the gastrocnemius temperature of the sheep in the chronic cold stress group is significantly lower than 0.87 ℃, 0.61 ℃ and the temperature difference is 0.87 ℃ at the maximum at 8:00 and 9:00 respectively.
The experimental results show that the daily gain of the chronic cold stress sheep is negative, the weight is reduced, and the heart rate is obviously increased. In particular, the trends in tissue temperature (longissimus dorsi and gastrocnemius) were consistent with rectal temperature and were lower than the control group at the time points measured, further suggesting that the metabolic thermogenesis process in sheep was not sufficient to maintain the same temperature as the control group sheep, and the sheep were in stress. These results demonstrate that continuous gradient cooling successfully induces a sheep model of chronic cold stress.
In conclusion, the establishment of the method lays a foundation condition for deeply researching the behavior change, the physiological characteristics and the molecular regulation of the sheep under the chronic cold stress.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A construction method of a sheep chronic cold stress model is characterized by comprising the following steps: in a feeding room for feeding sheep to be treated, the temperature in the feeding room is continuously and gradiently cooled to-35 ℃ to-20 ℃, and the temperature is kept for 20-25 days at constant temperature.
2. The construction method according to claim 1, wherein the continuous gradient cooling is to reduce the temperature once every two days, and each time, the temperature is reduced by 4-6 ℃.
3. The method of claim 2, wherein the temperature is lowered by 5 ℃ each time.
4. The constructing method according to claim 3, wherein the initial temperature of the rearing chamber is controlled to 20 ℃ ± 0.5 ℃.
5. The constructing method according to claim 4, wherein the initial temperature in the feeding chamber is set to 20 ℃, the temperature is reduced by 5 ℃ every two days, and the temperature is kept constant at-20 ℃ for 23-25 days.
6. The method of claim 5, wherein the temperature is maintained at-20 ℃ for 24 days.
7. The construction method according to claim 1, wherein the temperature in the feeding chamber is reduced to the target temperature within 20-30min during the continuous gradient temperature reduction.
8. The construction method according to claim 1, wherein the temperature reduction adjustment is performed at 9-10 am;
preferably, the cooling regulation is performed at 9 a.m.
9. Use of a model of chronic cold stress in sheep constructed by a method according to any one of claims 1 to 8 in the study of chronic cold stress in sheep for the purpose of non-disease diagnosis or treatment.
10. Use of the sheep chronic cold stress model constructed by the construction method according to any one of claims 1 to 8 in screening sheep anti-chronic stress response drugs or acute stress response drugs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210591793.3A CN114847227A (en) | 2022-05-27 | 2022-05-27 | Construction method and application of sheep chronic cold stress model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210591793.3A CN114847227A (en) | 2022-05-27 | 2022-05-27 | Construction method and application of sheep chronic cold stress model |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114847227A true CN114847227A (en) | 2022-08-05 |
Family
ID=82640746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210591793.3A Pending CN114847227A (en) | 2022-05-27 | 2022-05-27 | Construction method and application of sheep chronic cold stress model |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114847227A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444439A (en) * | 2008-12-22 | 2009-06-03 | 山东大学 | Method for preparing hypertension mouse model |
| CN103184189A (en) * | 2011-12-29 | 2013-07-03 | 北京富通华生物科技有限公司 | Cultivation method of cross-bred wagy |
| CN108029637A (en) * | 2018-01-31 | 2018-05-15 | 浙江大学 | A kind of method that Layer Production Performance is maintained under the conditions of cold stress |
| CN208783582U (en) * | 2018-09-03 | 2019-04-26 | 四川农业大学 | A kind of anti-cold stress detection device of drosophila |
| CN111493026A (en) * | 2020-04-30 | 2020-08-07 | 苏州必睿康医药科技有限公司 | Construction method of non-human animal model for type 2 diabetes and application of obtained animal |
-
2022
- 2022-05-27 CN CN202210591793.3A patent/CN114847227A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101444439A (en) * | 2008-12-22 | 2009-06-03 | 山东大学 | Method for preparing hypertension mouse model |
| CN103184189A (en) * | 2011-12-29 | 2013-07-03 | 北京富通华生物科技有限公司 | Cultivation method of cross-bred wagy |
| CN108029637A (en) * | 2018-01-31 | 2018-05-15 | 浙江大学 | A kind of method that Layer Production Performance is maintained under the conditions of cold stress |
| CN208783582U (en) * | 2018-09-03 | 2019-04-26 | 四川农业大学 | A kind of anti-cold stress detection device of drosophila |
| CN111493026A (en) * | 2020-04-30 | 2020-08-07 | 苏州必睿康医药科技有限公司 | Construction method of non-human animal model for type 2 diabetes and application of obtained animal |
Non-Patent Citations (3)
| Title |
|---|
| 张杉洲等: "《最新公园动物园动物科学饲养技术与疾病防疫及常见疾病诊治应用手册第1卷》", vol. 1, 30 September 2004, 银声音像出版社, pages: 188 * |
| 苏莹莹: "《长期冷刺激对肉仔鸡环境适应性的影响》", 《中国博士学位论文全文数据库农业科技辑》, no. 09, pages 050 - 15 * |
| 高静雯: "《寒冷应激对阿勒泰羔羊及杂交种脂质能量代谢通路及免疫功能的影响》", 《中国优秀硕士学位论文全文数据库农业科技辑》, no. 01, pages 05 - 199 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fuchs et al. | Heart rate sensor validation and seasonal and diurnal variation of body temperature and heart rate in domestic sheep | |
| Giuseppe et al. | Biological rhythm in livestock | |
| Allden et al. | The determinants of herbage intake by grazing sheep: the interrelationship of factors influencing herbage intake and availability | |
| Kelly et al. | Digestive development of the early-weaned pig: 1. Effect of continuous nutrient supply on the development of the digestive tract and on changes in digestive enzyme activity during the first week post-weaning | |
| Wilson et al. | Modification in the sexual behavior of male rats produced by changing the stimulus female. | |
| Fashina-Bombata et al. | Influence of salinity on the developmental stages of African catfish Heterobranchus longifilis (Valenciennes, 1840) | |
| Gray | The mechanism of ciliary movement. III.—The effect of temperature | |
| Mitchell et al. | The effect of age, sex, and castration on the basal heat production of chickens | |
| Rasmussen | Changes in the activity budgets of yellow baboons (Papio cynocephalus) during sexual consortships | |
| Bodey et al. | Longitudinal studies of reproducibility and variability of indirect (oscillometric) blood pressure measurements in dogs: evidence for tracking | |
| Hulbert | Metabolism and the development of endothermy | |
| CN114847227A (en) | Construction method and application of sheep chronic cold stress model | |
| Mzilikazi et al. | Lack of torpor in free-ranging southern lesser galagos, Galago moholi: ecological and physiological considerations | |
| Gaddour et al. | Growth curve estimation in pure goat breeds and crosses of first and second generation in Tunisian oases | |
| Wagnon et al. | Bovine laterality | |
| Pulikanti et al. | Physiological responses of broiler embryos to in ovo implantation of temperature transponders | |
| Swiergiel et al. | Effect of diet and temperature acclimation on thermoregulatory behaviour in piglets | |
| Husain et al. | Pasture management to minimise the detrimental effects of pre‐lamb shearing | |
| Broom | The effects of biotechnology on animal welfare | |
| Byrd et al. | Measuring piglet castration pain using linear and non-linear measures of heart rate variability | |
| Broom | Assessing the welfare of transgenic animals | |
| Archer et al. | Differences in the acute pain responses of two breeds of lamb following castration and tail docking with the rubber ring method | |
| Wiesner | The experimental study of senescence | |
| ULOMOVICH et al. | Morphogenesis Of The Hind Leg Distal Muscles Of Hissar Sheep Of Different Breeds In Different Ecological Conditions | |
| Godoi et al. | Physiological and behavioral response of foals to hot iron or freeze branding |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220805 |