CN114839285A - Method for analyzing characteristic compounds of tobacco essence and application thereof - Google Patents

Method for analyzing characteristic compounds of tobacco essence and application thereof Download PDF

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Publication number
CN114839285A
CN114839285A CN202210433799.8A CN202210433799A CN114839285A CN 114839285 A CN114839285 A CN 114839285A CN 202210433799 A CN202210433799 A CN 202210433799A CN 114839285 A CN114839285 A CN 114839285A
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China
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analyzing
tobacco
reagent
characteristic
mixing
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Inventor
马梦婕
李炜
付金存
韩云龙
郭宏霞
刘琪
袁益来
朱莹
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China Tobacco Jiangsu Industrial Co Ltd
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China Tobacco Jiangsu Industrial Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/30—Control of physical parameters of the fluid carrier of temperature
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7206—Mass spectrometers interfaced to gas chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/025—Gas chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N2030/042—Standards
    • G01N2030/045—Standards internal
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/067—Preparation by reaction, e.g. derivatising the sample

Abstract

The invention provides a method for analyzing a characteristic compound of tobacco essence and application thereof, wherein the method for analyzing the characteristic compound of the tobacco essence comprises the following steps: mixing a sample to be detected with an internal standard substance, then drying, mixing and reacting with an oximation reagent, mixing and reacting with a silanization reagent, and finally detecting by GC-MS to obtain a result; the oximation reagent is a combination of methoxylamine hydrochloride and hydroxylamine hydrochloride. The analysis method provided by the invention can efficiently and quickly detect the characteristic compounds in the tobacco essence, and has accurate detection result and high precision.

Description

Method for analyzing characteristic compounds of tobacco essence and application thereof
Technical Field
The invention belongs to the field of analysis, particularly relates to a method for analyzing a characteristic compound of tobacco essence and application thereof, and particularly relates to an accurate method for analyzing the characteristic compound of the tobacco essence and application thereof.
Background
The characteristic compound of the tobacco essence is mainly water-soluble saccharide. The water-soluble sugar is an important primary metabolite in the growth and development process of the tobacco, and not only is directly related to photosynthesis and respiration of the tobacco, but also directly influences the quality and quality of the tobacco. The content of the water-soluble sugar is closely related to the fragrance, the irritation and the tar generation of the tobacco, for example, the water-soluble reducing sugar and the amino compound can be combusted to generate furan Maillard reaction products, so that the quality of the tobacco can be effectively improved, the wood miscellaneous gas is reduced, and the tobacco fragrance is increased. In addition, tobacco with high sugar content also causes an increase in toxic and carcinogenic substances in the mainstream smoke, mainly formaldehyde, acetaldehyde, acetone, acrolein, and the like. Acetaldehyde has also been reported to increase smoker resistance to nicotine. The difference of the water-soluble sugar content in the tobacco of different varieties, parts and production places also causes the obvious difference of the compatibility of the tobacco leaves. At present, a detection method for water-soluble sugar in tobacco is relatively mature, but no universal method for detecting characteristic components in tobacco products such as tobacco essence exists. Therefore, how to provide an accurate and effective analysis method for characteristic components in the tobacco essence becomes a problem to be solved urgently.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method for analyzing a characteristic compound of tobacco essence and application thereof, and particularly provides an accurate method for analyzing the characteristic compound of the tobacco essence and application thereof. The analysis method provided by the invention can efficiently and quickly detect the characteristic compounds in the tobacco essence, and has accurate detection result and high precision.
In order to achieve the purpose, the invention adopts the following technical scheme:
on one hand, the invention provides a method for analyzing tobacco essence characteristic compounds, which comprises the following steps:
mixing a sample to be detected with an internal standard substance, then blowing the mixture to be dried, mixing the mixture with an oximation reagent for reaction, then mixing the mixture with a silanization reagent for reaction, and finally detecting the mixture by GC-MS to obtain a result.
The oximation reagent is a combination of methoxylamine hydrochloride and hydroxylamine hydrochloride.
According to the method, the specific oximation reagent methoxylamine hydrochloride and hydroxylamine hydrochloride are compounded and act synergistically, so that characteristic compounds in the tobacco essence can be effectively oximated, and the accuracy and precision of detection and analysis are improved.
Wherein the characteristic compounds are water-soluble saccharides and other substances, including lactic acid, benzyl alcohol, glycerol, pinoresinol, fructose, mannitol, sucrose, maltose, etc.
Preferably, the internal standard comprises palmitic acid-d 31 Myristic acid-d 27 Or tridecanoic acid-d 25 Any one of them.
Preferably, the temperature of the mixing reaction with the oximation agent is 25-35 ℃.
Preferably, the time for mixing and reacting with the oximation reagent is 80-120 min.
Preferably, the mass ratio of the sample to be detected to the oximation reagent is (200- & ltSUB- & gt 300) & gt 1.
Preferably, the mass ratio of the methoxylamine hydrochloride to the hydroxylamine hydrochloride is 5 (1-2).
The temperature of the mixing reaction with the oximation agent can be 25 ℃, 26 ℃, 27 ℃, 28 ℃, 29 ℃, 30 ℃, 31 ℃, 32 ℃, 33 ℃, 34 ℃ or 35 ℃, the time can be 80min, 90min, 100min, 110min or 120min, the mass ratio of the sample to be tested to the oximation agent can be 200:1, 220:1, 240:1, 260:1, 280:1 or 300:1, the mass ratio of the methoxyamine hydrochloride to the hydroxylamine hydrochloride can be 5:1, 5:1.5 or 5:2, but the above-mentioned values are not limited, and other values in the above-mentioned value range are also applicable.
Preferably, the silylating agent is a combination of BSTFA (bis (trimethylsilyl) trifluoroacetamide), MSTFA (N-methyl-N- (trimethylsilane) trifluoroacetamide), and MTBSTFA (N- (t-butyldimethylsilyl) -N-methyltrifluoroacetamide).
The silanization reagent is compounded by BSTFA, MSTFA and MTBSTFA, and under the synergistic action, an oximated sample to be detected can be sufficiently silanized, so that the detection accuracy and precision are further improved.
Preferably, the mass ratio of BSTFA, MSTFA and MTBSTFA is (1-2): (1-2): 1-2).
Preferably, the ratio of the mass of the sample to be detected to the volume of the silylation reagent is 1 (400-600) g/muL.
Preferably, the temperature for mixing and reacting with the silanization reagent is 30-40 ℃ and the time is 50-70 min.
In the mass ratio of BSTFA, MSTFA and MTBSTFA, the number of BSTFA may be 1, 1.2, 1.4, 1.6, 1.8 or 2, the number of MSTFA may be 1, 1.2, 1.4, 1.6, 1.8 or 2, the number of MTBSTFA may be 1, 1.2, 1.4, 1.6, 1.8 or 2, the ratio of the mass of the sample to the volume of the silylation reagent may be 1:400 g/. mu.L, 1:450 g/. mu.L, 1:500 g/. mu.L, 1:550 g/. mu.L or 1:600 g/. mu.L, and the reaction time with the silylation reagent may be 30 ℃, 31 ℃, 32 ℃, 33 ℃, 34 ℃, 35 ℃, 36 ℃, 37 ℃, 38 ℃, 39 ℃ or 40 ℃, and the time may be 50min, 55min, 60min, 65min or 70min, but is not limited to the values listed above, and other values in the same range may be applied.
In another aspect, the present invention also provides the use of an assay as described above in the testing of tobacco products.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for analyzing characteristic compounds of tobacco essence, which is characterized in that a specific oximation reagent, namely methoxylamine hydrochloride and hydroxylamine hydrochloride, are adopted, and the specific oximation reagent, namely methoxylamine hydrochloride and hydroxylamine hydrochloride are compounded and act synergistically, so that the characteristic compounds in the tobacco essence can be effectively oximated, and the accuracy and precision of detection and analysis are improved; and by adopting the compounding of BSTFA, MSTFA and MTBSTFA and the synergistic action, the oximated sample to be detected can be fully silanized, and the detection accuracy and precision are further improved.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following further describes the technical solution of the present invention with reference to the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
In the following examples, the sample to be tested is peru extract, which is purchased from huabao essence, inc.
Example 1
The embodiment provides a method for analyzing a characteristic compound of tobacco essence, which comprises the following specific steps:
weighing 0.2g of sample to be tested, accurately weighing 0.0001g of sample to be tested, and placing the sample in a 10mL centrifuge tube. Accurately add 0.2mL palmitic acid-d separately 31 The solution (5g/L) and 5mL of methanol were vortexed and shaken for 20min, filtered through a 0.22 μm organic phase filter, and then 100 μ L of the solution was dried with nitrogen. Then, 30. mu.L of a 20mg/mL solution of methoxylamine hydrochloride in pyridine and 10. mu.L of 18mg/mL solution of hydroxylamine hydrochloride in ethanol were added, and after vortexing for 2 minutes, the mixture was reacted at 30 ℃ for 100 minutes, and then 30. mu.L of BSTFA, 30. mu.L of MSTFA and 30. mu.L of MTBSTFA were added, and after vortexing for 30 seconds, the mixture was reacted at 37 ℃ for 60 minutes. And finally transferring the derivatized solution into a micro sample injection device for GC-MS analysis.
Specification of chromatographic column: 30m × 0.25mm × 0.25 μm, and the stationary phase is (5% -phenyl) -methyl polysiloxane; carrier gas: helium (4.1); sample inlet temperature: 290 ℃; temperature programming: the initial temperature is 80 ℃, the temperature is kept for 3min, the temperature is increased to 310 ℃ at the speed of 8 ℃/min, and the temperature is kept for 10 min.
The detection results are as follows:
serial number Retention time/min Name of substance Content/mg
1 11.374 Lactic acid 0.74
2 14.003 Benzyl alcohol 16.11
3 17.487 Glycerol 0.6
4 31.141 Pinecone alcohol 1.85
5 31.651 Fructose 1 0.36
6 32.167 Fructose 2 5.97
7 33.288 Mannitol 0.15
8 45.292 Sucrose 0.86
9 46.838 Maltose 1 66.14
10 47.224 Maltose 2 12.88
The results fully show that the analysis method provided by the invention can effectively detect the content of the characteristic compounds in the tobacco essence (wherein fructose 1 and fructose 2, maltose 1 and maltose 2 are isomers).
Example 2
The embodiment provides a method for analyzing characteristic compounds of tobacco flavor, which is consistent with embodiment 1 except that BSTFA is not added in the specific steps, and a reduced part is proportionally distributed to MSTFA and MTBSTFA.
Example 3
The embodiment provides a method for analyzing characteristic compounds of tobacco flavor, which is consistent with embodiment 1 except that MSTFA is not added in the specific steps, and a reduced part is proportionally distributed to BSTFA and MTBSTFA.
Example 4
The embodiment provides an analysis method of characteristic compounds of tobacco flavors, which is the same as the embodiment 1 except that MTBTSFA is not added in the specific steps and the reduced part is proportionally distributed to MSTFA and BSTFA.
Example 5
The embodiment provides a method for analyzing characteristic compounds of tobacco flavor, which is consistent with the embodiment 1 except that MSTFA and BSTFA are not added in the specific steps and part of the MSTFA and the BSTFA are reduced and distributed to MTBTFA.
Example 6
The embodiment provides a method for analyzing characteristic compounds of tobacco flavor, which is consistent with the embodiment 1 except that MTBTFA and BSTFA are not added in the specific steps and part of the MTBTFA and BSTFA is reduced and distributed to MSTFA.
Example 7
The embodiment provides a method for analyzing characteristic compounds of tobacco flavor, which is consistent with the embodiment 1 except that MSTFA and MTBSSTFA are not added in the specific steps and part of the MSTFA and MTBSSTFA are reduced.
Comparative example 1
The comparative example provides a method for analyzing a characteristic compound of tobacco essence, and the method is consistent with the example 1 except that methoxylamine hydrochloride is not added and part of hydroxylamine hydrochloride is reduced in the specific steps.
Comparative example 2
The comparative example provides a method for analyzing a characteristic compound of tobacco essence, and the method is consistent with the example 1 except that hydroxylamine hydrochloride is not added in the specific steps and part of hydroxylamine hydrochloride is reduced and distributed to methoxylamine hydrochloride.
The methods of examples 1-7 and comparative examples 1-2 were repeated 6 times the same day, and the in-day precision was calculated as follows (using lactic acid as an example):
group of Example 1 Example 2 Example 3 Example 4 Example 5
RSD/％ 2.49 3.54 2.97 3.48 3.62
Group of Example 6 Example 7 Comparative example 1 Comparative example 2
RSD/％ 4.03 3.88 4.35 3.94
The methods of examples 1-7 and comparative examples 1-2 were then repeated once daily for a total of five days, with the results calculated as follows (using lactic acid as an example):
group of Example 1 Example 2 Example 3 Example 4 Example 5
RSD/％ 2.21 3.48 3.21 3.67 3.43
Group of Example 6 Example 7 Comparative example 1 Comparative example 2
RSD/％ 4.21 3.72 4.16 4.16
The results show that the method provided by the invention has the advantages that by adopting the compounding of methoxylamine hydrochloride and hydroxylamine hydrochloride and the compounding of BSTFA, MSTFA and MTBSSTFA, the daily precision and the daytime precision of the analysis method are obviously improved, and the accuracy of the detection result is improved.
The applicant states that the method for analyzing the characteristic compounds of the tobacco flavor and the application thereof are described by the above examples, but the invention is not limited to the above examples, that is, the invention is not limited to the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.

Claims (10)

1. A method for analyzing tobacco essence characteristic compounds is characterized by comprising the following steps:
mixing a sample to be detected with an internal standard substance, then drying, mixing and reacting with an oximation reagent, mixing and reacting with a silanization reagent, and finally detecting by GC-MS to obtain a result;
the oximation reagent is a combination of methoxylamine hydrochloride and hydroxylamine hydrochloride.
2. The method for analyzing the characteristic compounds of the tobacco flavor according to claim 1, wherein the internal standard substance comprises palmitic acid-d 31 Myristic acid-d 27 Or tridecanoic acid-d 25 Any one of them.
3. The method for analyzing the characteristic compounds of the tobacco flavor according to claim 1 or 2, wherein the temperature for mixing and reacting with the oximation reagent is 25-35 ℃.
4. The analysis method of the cigarette flavor characteristic compound according to any one of claims 1 to 3, wherein the time for mixing and reacting with the oximation reagent is 80 to 120 min.
5. The method for analyzing the characteristic compounds of the tobacco flavor according to any one of claims 1 to 4, wherein the mass ratio of the sample to be tested to the oximation reagent is (200- & 300) & 1.
6. The method for analyzing the characteristic compounds of the tobacco flavor according to any one of claims 1 to 5, wherein the mass ratio of the methoxylamine hydrochloride to the hydroxylamine hydrochloride is 5 (1-2).
7. The method for analyzing the tobacco flavor characteristic compound according to any one of claims 1 to 6, wherein the silylation reagent is a combination of BSTFA, MSTFA and MTBSTFA;
preferably, the mass ratio of BSTFA, MSTFA and MTBSTFA is (1-2): (1-2): 1-2).
8. The method for analyzing the characteristic compounds of the tobacco flavor according to any one of claims 1 to 7, wherein the ratio of the mass of the sample to be tested to the volume of the silylation reagent is 1 (400) g/μ L.
9. The analysis method of the tobacco flavor characteristic compound according to any one of claims 1 to 8, wherein the temperature of the mixing reaction with the silylation reagent is 30 to 40 ℃ and the time is 50 to 70 min.
10. Use of the method for the analysis of a characteristic compound of tobacco flavor according to claims 1 to 9 in the inspection of tobacco products.
CN202210433799.8A 2022-04-24 2022-04-24 Method for analyzing characteristic compounds of tobacco essence and application thereof Pending CN114839285A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102590412A (en) * 2012-01-18 2012-07-18 中国烟草总公司郑州烟草研究院 Method for detecting secondary metabolites in fresh tobacco leaves by using derivatization GC-MS
CN104458934A (en) * 2014-10-24 2015-03-25 贵州省烟草科学研究院 Method for detecting water-soluble sugar in tobacco and tobacco products
CN105092750A (en) * 2014-05-12 2015-11-25 中国科学院大连化学物理研究所 Method for judging quality of fresh tobacco leaf sample in tobacco metabonomics research and kit
CN106483217A (en) * 2016-09-30 2017-03-08 中国烟草总公司郑州烟草研究院 A kind of method that GC MS detects nascent metabolite and secondary metabolitess in fresh tobacco leaves
CN111366671A (en) * 2019-04-25 2020-07-03 中南民族大学 Chemical derivatization-ultra-high performance liquid chromatography-tandem mass spectrometry for simultaneously detecting 18 steroid hormones in serum

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102590412A (en) * 2012-01-18 2012-07-18 中国烟草总公司郑州烟草研究院 Method for detecting secondary metabolites in fresh tobacco leaves by using derivatization GC-MS
CN105092750A (en) * 2014-05-12 2015-11-25 中国科学院大连化学物理研究所 Method for judging quality of fresh tobacco leaf sample in tobacco metabonomics research and kit
CN104458934A (en) * 2014-10-24 2015-03-25 贵州省烟草科学研究院 Method for detecting water-soluble sugar in tobacco and tobacco products
CN106483217A (en) * 2016-09-30 2017-03-08 中国烟草总公司郑州烟草研究院 A kind of method that GC MS detects nascent metabolite and secondary metabolitess in fresh tobacco leaves
CN111366671A (en) * 2019-04-25 2020-07-03 中南民族大学 Chemical derivatization-ultra-high performance liquid chromatography-tandem mass spectrometry for simultaneously detecting 18 steroid hormones in serum

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
宗永立 等: "混合型卷烟加料加香技术研究", 烟草科技, no. 03, pages 3 - 8 *
张丽 等: "植物代谢组学及其在烟草上的应用进展", 中国烟草学报, vol. 21, no. 05, pages 126 - 134 *
方锴 等: "类固醇类激素GC-MS分析中酮基衍生化技术研究进展", 分析化学, vol. 38, no. 05, pages 743 - 751 *
杜启云 等: "卷烟中非挥发性有机酸、糖类、某些氨基酸和磷酸的同时测定", 烟草科技, no. 7, pages 30 - 35 *
赵媛媛 等: "气相色谱中的衍生试剂及新进展", 分析科学学报, vol. 19, no. 01, pages 92 - 96 *

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