CN114839172A - Optical manipulation and fluorescence imaging system and imaging method - Google Patents
Optical manipulation and fluorescence imaging system and imaging method Download PDFInfo
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- CN114839172A CN114839172A CN202210417159.8A CN202210417159A CN114839172A CN 114839172 A CN114839172 A CN 114839172A CN 202210417159 A CN202210417159 A CN 202210417159A CN 114839172 A CN114839172 A CN 114839172A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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Abstract
The invention discloses an optical control and fluorescence imaging system and an imaging method, wherein the imaging system comprises an optical control light source, a fluorescence detection unit and a fluorescence detection unit, wherein the optical control light source is used for generating first laser in a first direction; the spatial light modulation unit is used for adjusting an included angle between the first laser and the first direction and projecting the first laser to the observed sample; the fluorescence excitation light source group is used for generating second laser and projecting the second laser to the observed sample; a sample end lens movable in a second direction to approach or depart from the observed sample for changing the focus position of the first laser light in the second direction, wherein the second direction is vertical to the image plane of the sample end lens; and the imaging unit receives the fluorescence signal of the observed sample. The imaging system of the invention realizes the precise optical control of the observed biological cells in the fluorescence imaging field of view.
Description
Technical Field
The present invention relates to the field of fluorescence imaging technology, and more particularly, to an optical control and fluorescence imaging system and method.
Background
The fluorescence imaging technology realizes imaging by using biological cells and tissues specifically marked by a fluorescent probe. The wide-spectrum fluorescence imaging band including visible light (400-650nm), near-infrared first-region (NIR-I650-900 nm) and near-infrared second-region fluorescence (NIR-II 1000-1700nm) is widely applied to the research of life science. However, when the single fluorescence imaging technology is applied to the study of thick tissues and living organisms, the imaging of the image plane is interfered by fluorescence emitted from a non-image plane due to the limited depth of field of the imaging objective lens; meanwhile, the single fluorescence imaging cannot realize the manipulation (opening of ion channels) of the observed cells on the image plane.
The optical manipulation technology is to stimulate biological cells by light with specific wavelength regulation, and to manipulate the cells in living animals in real time with millisecond-level time precision and subcellular-level space precision, and has been widely applied to biomedical research (for example, optogenetic technologies based on ion channel proteins ChR, Arch and NpHR can regulate and control the switching of different ion channels of nerve cells to activate nerve excitation or inhibit nerve excitation).
However, no device is available at present which can realize accurate optical control of observed biological cells in a fluorescence imaging field of view and the optical control and the fluorescence imaging are linked in real time. In particular, an imaging device which does not have the combination of a near infrared optical manipulation technology and a near infrared II-zone fluorescence imaging technology with high tissue penetration depth and high space-time resolution exists.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide an optical control and fluorescence imaging system, which can realize accurate optical control on observed biological cells in a fluorescence imaging visual field, reduce fluorescence crosstalk of different planes and acquire richer information of the observed biological cells.
In order to achieve the above object, an embodiment of the present invention provides an optical steering and fluorescence imaging system, which includes an optical steering light source, a spatial light modulation unit, a fluorescence excitation light source set, a sample end lens, and an imaging unit.
The optical control light source is used for generating first laser in a first direction; the spatial light modulation unit adjusts an included angle between the first laser and the first direction and projects the first laser to an observed sample; the fluorescence excitation light source group is used for generating second laser and projecting the second laser onto the observed sample; the sample end lens can move in a second direction to approach or depart from the observed sample so as to change the focus position of the first laser in the second direction, and the second direction is vertical to the image plane of the sample end lens; the imaging unit receives a fluorescence signal of an observed sample.
In one or more embodiments of the invention, the imaging system further includes an imaging end lens, and the imaging end lens is matched with the sample end lens to transmit the fluorescence signal of the observed sample to the imaging unit in an object-image conjugate relation.
In one or more embodiments of the present invention, the imaging system further includes a filter structure disposed at a front end of the imaging end lens for filtering stray light of non-fluorescent signals.
In one or more embodiments of the present invention, the imaging system further includes a dichroic mirror group disposed on an optical path from the optical manipulation light source and the fluorescence laser light source group to coaxially couple the first laser light and the second laser light and guide the first laser light and the second laser light into a sample end lens.
In one or more embodiments of the present invention, the spatial light modulation unit is disposed between the optical control light source and the dichroic mirror group, and includes a spatial light modulator and a relay mirror group matched with the spatial light modulator, the spatial light modulator is sequentially disposed along the first laser emission path, the spatial light modulator is configured to achieve the first laser collimation and output the first laser at a certain angle, an output angle of the first laser collimated by the spatial light modulator is adjustable, and an adjustable range of the output angle is: and forms an angle of 0-40 degrees with the first direction.
In one or more embodiments of the present invention, the imaging system further includes a control unit, where the control unit is connected to the optical control light source, the spatial light modulation unit, the fluorescence excitation light source group, and the imaging unit, and is configured to control light emitting time sequences of the optical control light source and the fluorescence excitation light source group, change an output angle of the first laser light adjusted by the spatial light modulation unit, and control the imaging unit to acquire a fluorescence signal at a certain time sequence.
In one or more embodiments of the present invention, the control unit has an internal clock function, and can control the optical control light source, the fluorescence excitation light source group, the spatial light modulation unit and the imaging unit to cooperatively work in a time sequence within nanosecond precision.
In one or more embodiments of the present invention, the imaging system further includes a displacement device, the displacement device is connected to the sample end lens and the control unit, and the position of the sample end lens relative to the observed sample in the second direction can be adjusted under the control of the control unit.
In one or more embodiments of the present invention, the imaging unit may convert the received fluorescent signal into an image signal; the imaging system further comprises an input/output unit, wherein the input/output unit is connected with the control unit and the imaging unit and is used for sending a control instruction to the control unit and acquiring and displaying an image signal of the imaging unit.
In one or more embodiments of the present invention, the coverage wavelength band of the optical manipulation light source is 350-.
In one or more embodiments of the present invention, the fluorescence laser light source set includes a first laser, a light guide fiber and an integrator, wherein the excitation light generated by the first laser is transmitted to the integrator by the light guide fiber; and/or
The optical control light source comprises a second laser, an optical fiber and a mirror group, and control light generated by the second laser is transmitted to the mirror group through the optical fiber.
In one or more embodiments of the present invention, the imaging unit includes at least 2 sets of detectors, wherein one set of detectors is an indium gallium arsenic detector sensitive to the 900-1700nm waveband.
The embodiment of the present invention further provides an optical steering and wide spectrum fluorescence imaging method, which is based on the above-mentioned optical steering and fluorescence imaging system, and the imaging method includes:
generating second laser by the fluorescence excitation light source group and projecting the second laser to the observed sample to enable the observed sample to generate a fluorescence signal under the irradiation of the second laser;
the imaging unit receives the fluorescence signal of the observed sample in real time and forms a fluorescence image;
the method comprises the steps that a first laser is generated by an optical control light source and is projected onto an observed sample after passing through a spatial light modulation unit and a sample end lens;
the spatial light modulation unit is used for adjusting an included angle between the first laser and the first direction so as to change the XY spatial position of the first laser focused on the image plane of the sample end lens, and the position of the focus point of the first laser in the direction vertical to the image plane of the sample end lens is changed by adjusting the movement of the sample end lens so as to carry out light control on the observed sample.
Compared with the prior art, the optical control and fluorescence imaging system provided by the embodiment of the invention realizes accurate optical control on observed biological cells in a fluorescence imaging field.
The optical control and fluorescence imaging system provided by the embodiment of the invention realizes real-time linkage of optical control and fluorescence imaging, can reduce fluorescence crosstalk of different planes, and can acquire richer information of observed biological cells.
The optical control and fluorescence imaging system provided by the embodiment of the invention can be used for carrying out real-time control on an observed sample in an imaging field in combination with optical control while providing high-penetration-depth near-infrared optical imaging.
In the optical manipulation and fluorescence imaging system of the embodiment of the invention, the manipulation position of the optical manipulation realizes the variable of any XY spatial position in an image plane by controlling the spatial light modulator; the displacement device enables the sample end lens to move perpendicular to the observed sample, so that the z-axis position of the image plane can be changed, and the control of the control light on any XYZ spatial position of the observed sample can be realized.
According to the optical control and fluorescence imaging system, the fluorescence excitation, the optical control and information acquisition multi-event real-time linkage is realized through the control unit, so that the optical control and fluorescence imaging system has a better and wider application prospect.
Drawings
FIG. 1 is a diagram of an optical steering and fluorescence imaging system in accordance with an embodiment of the present invention;
FIG. 2 is a diagram of a method for optical manipulation and fluorescence imaging in accordance with an embodiment of the present invention.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.
The broad spectrum fluorescence refers to the fluorescence in the wavelength range of 350-1700 nm. Based on the wide application of fluorescence imaging technology and optical control technology in life science or biomedical research, but at present, no equipment can realize accurate optical control on observed biological cells in a fluorescence imaging field of view and the optical control and the fluorescence imaging are linked in real time. The invention creatively provides an optical control and fluorescence imaging system, in particular to a combined imaging system of a near-infrared optical control technology and a near-infrared II-zone fluorescence imaging technology, which realizes accurate optical control of observed biological cells in a fluorescence imaging field and real-time linkage of the optical control and the fluorescence imaging.
As shown in fig. 1, an embodiment of the present invention provides an optical steering and fluorescence imaging system, which includes an optical steering light source 1, a spatial light modulation unit 2, a fluorescence excitation light source group 3, a first dichroic mirror 30, a second dichroic mirror 31, a sample-side lens 20, an imaging-side lens 21, an imaging unit 22, a filtering structure 32, a displacement device 33, a control unit 24, and an input-output unit 25.
The coverage waveband of the fluorescence excitation light source group 3 is 350-1250nm, and is used for providing second laser which can excite the fluorescence probe in the observed sample A in the area 4 to be detected (for the convenience of understanding, the second laser is hereinafter generally referred to as excitation light because the second laser is used for exciting the fluorescence probe in the observed sample A); the excitation light is near-infrared excitation light or combined light of visible light and near-infrared excitation light. The fluorescence excitation light source group 3 comprises a first laser 3-1, a light guide optical fiber 3-2 and a light homogenizing mirror 3-3, wherein the first laser 3-1 is preferably an 808nm laser, and 808nm excitation light generated by the 808nm laser is transmitted to the light homogenizing mirror 3-3 through the light guide optical fiber 3-2.
The optical manipulation light source 1 is used for providing a first laser capable of performing optical manipulation (for the first laser is used for performing optical manipulation on the observed sample a, such as opening an ion channel of a biological cell of the observed sample a. for convenience of understanding, hereinafter, the first laser is collectively referred to as a manipulation light), and the coverage wavelength band thereof is 350-. The optical control light source 1 comprises a second laser 1-1, an optical fiber 1-2 and a mirror group 1-3, the second laser 1-1 is preferably a 980nm or 1064nm laser, and the 980nm or 1064nm control light generated by the second laser 1-1 is transmitted to the mirror group 1-3 through the optical fiber 1-2.
The spatial light modulation unit 2 is disposed between the optical manipulation light source 1 and the first dichroic mirror 30 and connected to the control unit 24. The spatial light modulation unit 2 comprises a spatial light modulator 2-1 and a relay lens group 2-2 matched with the spatial light modulator 2-1, the spatial light modulator 2-1 is sequentially arranged along the control light emitting path, the applicable wave band 350-1350nm of the spatial light modulator 2-1 can be a reflection type based on a micro mirror or a transmission type of liquid crystal, and the light energy utilization rate is more than or equal to 60%. The spatial light modulator 2-1 can realize collimation of the control light and output the control light at a certain angle, the output angle is adjustable, the adjustable range is any angle of 0-40 degrees with a first direction, and the first direction is the first laser emitting direction of the optical control light source 1.
The first dichroic mirror 30 and the second dichroic mirror 31 are sequentially arranged on the light path emitted by the optical control light source 1 and the fluorescence excitation light source group 3, and the first dichroic mirror 30 is used for realizing coaxial coupling of the control light and the excitation light; second dichroic mirror 31 is of a multi-channel design, and is configured to allow the control light and the excitation light to enter sample-side lens 20 or to perform fluorescence excitation and emission so as to allow the control light and the excitation light to enter imaging-side lens 21.
The sample end lens 20 is located on the optical path between the second dichroic mirror 31 and the area to be detected 4 and is disposed near the area to be detected 4, and the imaging end lens 21 is disposed near the imaging unit 24. The sample end lens 20 is matched with the imaging end lens 21, and is used for transmitting the fluorescence signal of the observed sample A to the imaging unit in an object-image conjugate relation. The sample end lens 20 is matched with the relay lens group 2-2 of the spatial light modulation unit 2, so that the control light can be focused on an image plane, and the size of a focused spot is 1-500 mu m.
The imaging unit 22 comprises at least 2 detectors, and at least one group of indium gallium arsenic detectors sensitive to 900-1700nm bands is included therein. In the present embodiment, the imaging unit 22 is composed of an InGaAs detector with response of 900-1700nm and a CCD with a wavelength of 350-900 nm. The imaging unit 22 is used for acquiring the 900-1700nm fluorescence signals excited by the observed sample A in the region 4 to be detected and converting the fluorescence signals into image signals.
The filter structure 32 is disposed at the front end of the imaging lens 21 and is used for filtering stray light of non-fluorescent signals. In the process of transmitting the fluorescence signal to the imaging unit 22, the filtering structure 32 filters out the stray light except the fluorescence signal, and a high-quality fluorescence image is obtained.
The displacement device 33 is connected to the sample end lens 20 and is used for adjusting the vertical movement of the sample end lens 20 relative to the area to be detected 4 so as to change the position of the control light focus.
The control unit 24 is connected to the optical control light source 1, the fluorescence excitation light source group 3, the spatial light modulation unit 2, the imaging unit 22, and the displacement device 33, and is configured to control light emitting timings of the optical control light source 1 and the fluorescence excitation light source group 3, change a control light output angle of the spatial light modulation unit 2, control the displacement device 33 to drive the sample end lens 20 to move to change a position where control light is focused, and control the imaging unit 22 to acquire an image signal at a certain timing.
The input-output unit 25 is connected to the control unit 24 and the imaging unit 22, and is configured to send a control instruction to the control unit 24 and acquire and display an image signal of the imaging unit 22.
As shown in fig. 2, an embodiment of the present invention further provides an imaging method of the above-mentioned optical steering and fluorescence imaging system, including:
the first laser 3-1 in the fluorescence excitation light source group 3 generates the excitation light 51, and the excitation light is transmitted to the dodging mirror 3-3 through the light guide optical fiber 3-2. The dodging mirror 3-3 homogenizes the exciting light 51 and projects the homogenized exciting light to the first dichroic mirror 30 and the second dichroic mirror 31 to perform twice reflection and enter the sample end lens 20; wherein the first dichroic mirror 30 reflects light less than 900nm and transmits light greater than 900 nm; second dichroic mirror 31 is multichannel.
The exciting light 51 is uniformly projected onto the observed sample A in the region 4 to be detected by the sample end lens 20, and the observed sample A can be a living biological cell (a fluorescent probe containing 350-1700nm waveband). The fluorescent probe in the living organism cell is excited by the exciting light 51 to generate the fluorescent signal 52 with the wave band of 350-1700nm, and the fluorescent signal 52 can be various wavelengths or one wavelength, but necessarily comprises the fluorescent signal with the wave band of 900-1700nm, so that the deep layer of the observed sample A can be imaged. The generated fluorescence signal 52 is transmitted to the imaging unit 22 in an object-image conjugate relationship by the sample-side lens 20 and the imaging-side lens 21. In the process of transmitting the fluorescence signal 52 to the imaging unit 22, the filtering structure 32 filters out the stray light except the fluorescence signal 52, and a high-quality fluorescence image 53 is obtained. The fluorescence image 53 is transmitted to the input/output unit 25 to be displayed.
The tester (user) sends a control program 54 to the control unit 24 based on the fluorescence image 53 displayed by the input-output unit 25. The control program 54 includes an I/O signal 55 sent to the optical control light source 1 to control the light output and power adjustment of the control light; sending a time sequence I/O signal 56 to the imaging unit 22 to control the imaging unit 22 to acquire in real time; an instruction 58 to adjust the beam direction of the manipulation light 57 is issued to the spatial light modulation unit 2.
The control unit 24 may use a multifunctional acquisition card (with analog I/O, digital I/O and counter/timer) with an internal clock function as a core device, and is implemented by labview programming, and the control unit 24 may send an I/O signal (TTL synchronization signal) to the optical control light source 1, the fluorescence excitation light source group 3, the spatial light modulation unit 2, and the imaging unit 22 with time sequence, and the time sequence has nanosecond precision, so as to control the optical control light source 1, the fluorescence excitation light source group 3, the spatial light modulation unit 2, and the imaging unit 22 to cooperatively work with the time sequence.
After the optical control light source 1 receives the I/O signal 55, control light 57 generated by the second laser 1-1 is transmitted to the mirror group 1-3 through the optical fiber 1-2; the steering light 57 output from the mirror groups 1-3 enters the spatial light modulation unit 2. The control light passing through the spatial light modulation unit 2 is reflected twice by the first dichroic mirror 30 and the second dichroic mirror 31 and enters the imaging end lens 20, at this time, the control light 57 is focused into an image plane of the observed sample a, and light control is realized on the observed sample a at the focusing point, for example, 1064nm light opens a biological cell ion channel.
After receiving the command 58, the spatial light modulation unit 2 changes the beam direction of the control light, so as to change the focusing XY space position of the control light on the image plane, thereby changing the XY space position of the light control, and at this time, the control light is focused on the biological cell in the image plane to realize the light control on the biological cell in the target region to be detected. The sample end lens 20 is moved by the displacement device 33 perpendicularly to the observed sample a, so that the z-axis position of the control light focus can be changed, and at the moment, the control light can control the XYZ arbitrary spatial position of the observed sample a.
The imaging unit 22 receives the time sequential I/O signals 56 for real-time fluorescence signal acquisition. The feedback instruction 60 changes the light intensity, pulse frequency, etc. of the fluorescence excitation light source group 3 according to the actual requirements of the observed sample a, and at this time, the fluorescence excitation, optical control, and multi-event real-time linkage of information acquisition are completed.
Compared with the prior art, the optical control and fluorescence imaging system provided by the embodiment of the invention realizes accurate optical control on observed biological cells in a fluorescence imaging field.
The optical control and fluorescence imaging system provided by the embodiment of the invention realizes real-time linkage of optical control and fluorescence imaging, can reduce fluorescence crosstalk of different planes, and can acquire richer information of observed biological cells.
The optical control and fluorescence imaging system provided by the embodiment of the invention can be used for carrying out real-time control on an observed sample in an imaging field in combination with optical control while providing high-penetration-depth near-infrared optical imaging.
In the optical manipulation and fluorescence imaging system of the embodiment of the invention, the manipulation position of the optical manipulation realizes the variable of any XY spatial position in an image plane by controlling the spatial light modulator; the sample end lens is enabled to move perpendicular to the observed sample through the displacement device, so that the position of the z axis of the image plane can be changed, and the control of the control light on the XYZ arbitrary space position of the observed sample can be realized.
The optical control and fluorescence imaging system provided by the embodiment of the invention realizes real-time linkage of fluorescence excitation, optical control and information acquisition multiple events through the control unit, so that the optical control and fluorescence imaging system has better and wider application prospect.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. An optical steering and fluorescence imaging system, comprising:
an optical control light source for generating a first laser in a first direction;
the spatial light modulation unit is used for adjusting an included angle between the first laser and the first direction and projecting the first laser to the observed sample;
the fluorescence excitation light source group is used for generating second laser and projecting the second laser to the observed sample;
a sample end lens movable in a second direction to approach or depart from the observed sample for changing the focus position of the first laser light in the second direction, wherein the second direction is vertical to the image plane of the sample end lens;
and the imaging unit receives the fluorescence signal of the observed sample.
2. The optical steering and fluorescence imaging system of claim 1, further comprising an imaging end lens, wherein the imaging end lens cooperates with the sample end lens to transmit the fluorescence signal of the observed sample to the imaging unit in an object-image conjugate relationship.
3. The optical steering and fluorescence imaging system of claim 1, further comprising a set of dichroic mirrors disposed in an optical path from the optical steering light source and the set of fluorescence excitation light sources to coaxially couple the first laser light and the second laser light and direct the first laser light and the second laser light into a sample end lens.
4. The optical steering and fluorescence imaging system according to claim 3, wherein the spatial light modulation unit is disposed between the optical steering light source and the dichroic mirror set, the spatial light modulation unit includes a spatial light modulator and a relay mirror set matched with the spatial light modulator, the spatial light modulator is sequentially disposed along the first laser emission path, the spatial light modulator is configured to achieve the first laser collimation and output the first laser at an angle, and the output angle of the first laser collimated by the spatial light modulator is adjustable, and the adjustable range is: and forms an angle of 0-40 degrees with the first direction.
5. The optical manipulation and fluorescence imaging system according to claim 1, further comprising a control unit, wherein the control unit is connected to the optical manipulation light source, the spatial light modulation unit, the fluorescence excitation light source set and the imaging unit, and configured to control light emitting timings of the optical manipulation light source and the fluorescence excitation light source set, change an output angle of the first laser light adjusted by the spatial light modulation unit, and control the imaging unit to acquire a fluorescence signal at a certain timing.
6. The optical steering and fluorescence imaging system of claim 5, further comprising a displacement device coupled to the sample end lens and the control unit, wherein the position of the sample end lens in the second direction relative to the observed sample is adjustable under the control of the control unit.
7. The optically steered and fluoroscopic imaging system according to claim 5, wherein the imaging unit is capable of converting the received fluoroscopic signals into image signals; the imaging system further comprises an input/output unit, wherein the input/output unit is connected with the control unit and the imaging unit and is used for sending a control instruction to the control unit and acquiring and displaying an image signal of the imaging unit.
8. The optical manipulation and fluorescence imaging system of claim 1, wherein the optical manipulation light source has a wavelength band of 350-.
9. The optical steering and fluorescence imaging system of claim 8, wherein the fluorescence excitation light source set comprises a first laser, a light guide fiber and an integrator, wherein the excitation light generated by the first laser is transmitted to the integrator by the light guide fiber; and/or
The optical control light source comprises a second laser, an optical fiber and a mirror group, and control light generated by the second laser is transmitted to the mirror group through the optical fiber.
10. An optical steering and broad spectrum fluorescence imaging method based on the optical steering and fluorescence imaging system of any one of claims 1 to 9, the imaging method comprising:
generating second laser by the fluorescence excitation light source group and projecting the second laser to the observed sample to enable the observed sample to generate a fluorescence signal under the irradiation of the second laser;
the imaging unit receives the fluorescence signal of the observed sample in real time and forms a fluorescence image;
the method comprises the steps that a first laser is generated by an optical control light source and is projected onto an observed sample after passing through a spatial light modulation unit and a sample end lens;
the spatial light modulation unit is used for adjusting an included angle between the first laser and the first direction so as to change the XY spatial position of the first laser focused on the image plane of the sample end lens, and the position of the focus point of the first laser in the direction vertical to the image plane of the sample end lens is changed by adjusting the movement of the sample end lens so as to carry out light control on the observed sample.
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| CN115316961A (en) * | 2022-10-14 | 2022-11-11 | 南京诺源医疗器械有限公司 | Fluorescent image probe and handheld image detector |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115316961A (en) * | 2022-10-14 | 2022-11-11 | 南京诺源医疗器械有限公司 | Fluorescent image probe and handheld image detector |
| CN115316961B (en) * | 2022-10-14 | 2023-02-24 | 南京诺源医疗器械有限公司 | Handheld fluorescence image probe and handheld image detector |
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