CN114788890A - Attapulgite-melanin hemostatic gel and preparation method and application thereof - Google Patents
Attapulgite-melanin hemostatic gel and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an attapulgite-melanin hemostatic gel and a preparation method and application thereof, wherein the attapulgite-melanin hemostatic gel is compounded by an attapulgite-melanin composite component and a gel base material; the attapulgite-melanin composite component is compounded from attapulgite and natural melanin particles; firstly, uniformly mixing an attapulgite solution and a natural melanin particle solution in a water bath; then adding a chitosan solution, stirring, carrying out ice-water bath precooling, and then adding ammonium sulfate and tetramethyl ethylene diamine to prepare a gel precursor; finally, the attapulgite-melanin hemostatic gel is obtained by freezing reaction, unfreezing, washing and drying. The composite gel material is prepared by compounding the attapulgite-melanin composite component formed by compounding the attapulgite particles and the natural melanin particles with the gel base material, and the attapulgite particles and the natural melanin particles synergistically improve the hemostatic performance and have good biological safety.
Description
Technical Field
The invention belongs to the field of functional hemostatic materials, and particularly relates to attapulgite-melanin hemostatic gel and a preparation method and application thereof.
Background
The development and utilization of safe and efficient hemostatic materials are the key to the success of pre-hospital first aid. The main hemostatic components of the existing common hemostatic materials comprise chitosan, silicon dioxide, zeolite and the like. The chitosan hemostatic material has a porous structure and amino groups, can increase the adhesion of platelets and promote blood components such as red blood cells to aggregate at a wound site to form thrombus (Du, X, Liu, Y, Yan, H, et al, anti-infection and pro-complex hydrogel adhesive for less tissue and close. biomacromolecules 2020,21,1243 and 1253), but the application of the chitosan hemostatic material is limited because the chitosan hemostatic material is difficult to degrade and easily causes in vivo anaphylactic reaction. Mesoporous silica can promote the activation of coagulation factor XII and accelerate coagulation cascade to promote coagulation (Wang, C., Zhou, H., Niu, H., et al, tannic acid-loaded mesoporous silica for rapid homology and antibacterial activity, Biomaterials science.2018,6,3318-3331), but the potential metabolic toxicity and difficulty in cleaning powder particles at wounds limit the application thereof. The zeolite-based hemostatic material can adsorb water in blood at a wound to promote aggregation of platelets and coagulation factors, and release calcium ions to activate platelets to accelerate the coagulation process (Liang, y., Xu, c., Liu f., et al., experiment at least one of the coagulation in a biostatic vitamin interface of graphene span. acs Applied Materials interfaces, 2019, 2311, 23848-. Therefore, there is a need to develop a hemostatic material that combines rapid blood coagulation with biosafety.
Disclosure of Invention
Aiming at the defects of the existing hemostatic material, the invention aims to provide an attapulgite-melanin hemostatic gel and a preparation method and application thereof.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
an attapulgite-melanin hemostatic gel is prepared from attapulgite-melanin composite component and gel matrix; the attapulgite-melanin composite component is prepared by compounding attapulgite and natural melanin particles.
Further, the mass ratio of the attapulgite-melanin composite component to the gel base material is 0.2-1.2: 1.
further, the mass ratio of the attapulgite to the natural melanin granules is 10-100: 1.
further, the gel base material is selected from at least one of chitosan, gelatin and fibrin.
The invention also provides a preparation method of the attapulgite-melanin hemostatic gel, which comprises the steps of uniformly mixing the attapulgite solution and the natural melanin granule solution in water bath; then adding a chitosan solution, stirring, carrying out ice-water bath precooling, and then adding ammonium sulfate and tetramethyl ethylene diamine to prepare a gel precursor; finally, the attapulgite-melanin hemostatic gel is obtained by freezing reaction, unfreezing, washing and drying.
The attapulgite in the invention can be obtained by conventional treatments of attapulgite raw ore, such as water washing, ultrasonic stirring, multiple centrifugal separation and the like; the natural melanin granules can be extracted from animal hair, feathers, fungi, bacteria, etc., and can be obtained by, for example, diluting at least one of animal hair, feathers, fungi, bacteria in a phosphate buffer, performing enzymolysis or ultrasound, then performing water washing, centrifugation, drying, etc. by conventional treatment (refer to The emulsification of The biological structure of natural animal resources Journal of The Royal Society Interface,2018,15,20180045), and further preferably have a size of 200 to 400 nm. The treatment means of the attapulgite and the natural melanin granules in the invention are the prior conventional processes, and are not described again.
Further, the temperature of the water bath was 45-55 ℃.
Further, the mass concentration of the chitosan solution is 1-10 wt%, the stirring time is 12-48h, and the temperature of the ice water bath is 0-4 ℃.
Furthermore, the temperature of the freezing reaction is-10 to-20 ℃, and the reaction time is 24-48 h.
The invention also provides application of the attapulgite-melanin hemostatic gel as a hemostatic active component to preparation of emergency wound hemostatic materials, hemostatic products, wound repair materials or wound healing materials.
Compared with the prior art, the invention has the advantages that:
(1) in the attapulgite-melanin hemostatic gel, the attapulgite and melanin granules can promote adhesion and aggregation of red blood cells and platelets, activate the platelets, activate coagulation cascade reaction, synergistically accelerate the coagulation process and obviously improve the hemostatic performance of the gel material.
(2) In the attapulgite-melanin hemostatic gel, the porosity of the attapulgite-melanin hemostatic gel can quickly adsorb water in blood at a wound and concentrate blood coagulation substances, and meanwhile, a gel base material has a porous cross-linked structure similar to extracellular matrix, so that the attapulgite-melanin hemostatic gel is beneficial to migration and adhesion of cells and regeneration of wound tissues and promotes a healing process.
(3) In the attapulgite-melanin hemostatic gel, the gel base material, the attapulgite and the natural melanin particles have good biological safety, so that the prepared attapulgite-melanin hemostatic gel can realize safe and efficient hemostatic performance and is beneficial to the healing process of wounds.
Drawings
FIG. 1 is an SEM image of an attapulgite-melanin hemostatic gel (Pal-MGs HGs; example 2); the upper picture is an SEM picture of the overall porous morphology structure of the Pal-MGs HGs hemostatic gel, the SEM picture of the lower left corner presents an enlarged morphology structure of the pores of the Pal-MGs HGs hemostatic gel, and the SEM picture of the lower right corner presents a partially enlarged attapulgite and natural melanin particles of the pores of the Pal-MGs HGs hemostatic gel.
FIG. 2 is a bar graph of hemostasis time for different hemostatic materials; wherein: blank control group (NC), attapulgite-melanin hemostatic gel (Pal-MGs HGs; example 2), attapulgite (Pal; comparative example 1), natural melanin granules (MGs; comparative example 2), attapulgite-melanin composite powder (Pal-MGs; comparative example 3), commercial plain gauze (Medical gauze; comparative example 4).
Detailed Description
The present invention will be further described with reference to the following specific embodiments and the accompanying drawings, which are intended to make the technical features, scheme flows and innovation points of the present invention more clear. It should also be understood that the detailed description and specific examples are intended to provide illustration only, and that various other modifications and changes may occur to those skilled in the art upon reading this disclosure and are intended to be within the scope of the invention as claimed.
Example 1
Taking 30g of attapulgite (palygorskite) raw ore, dissolving in 800mL of deionized water (mass ratio of 3: 80), ultrasonically stirring for 8h, and carrying out suction filtration. And (3) re-suspending the attapulgite obtained by suction filtration in 300mL of deionized water, naturally settling for 10min, and taking the supernatant for centrifugal separation at the rotating speed of 8000 rpm. And (4) drying the precipitate obtained by centrifugation in an oven at 60 ℃ for 24 hours to obtain the attapulgite.
Commercially available feather or hair is taken, washed with acetone for 3 times, washed with deionized water for 3 times, and cut into pieces. The obtained feathers or hair were added to 40mL of phosphate buffer (pH 6.8), 0.4g of Dithiothreitol (DTT) was added thereto, and the mixture was mixed well under N 2 Shaking at 37 deg.C for 12h under atmosphere. Add 1mL of 20mg/mL proteinase K and 0.2g DTT and continue shaking for 12 h. Centrifuging at 8000rpm, washing with deionized water for 3 times, and removing supernatant to obtain precipitate. The resulting pellet was mixed with 0.4mL of proteinase K and 32mg of DTT in 16mL of phosphate buffer, resuspended, and shaken for 12 hours. Centrifuging, removing supernatant, adding 6mL of phosphate buffer and 0.12mL of polyethylene glycol octyl phenyl ether, oscillating for 6h, centrifuging, washing with methanol for 3 times and deionized water for 3 times in sequence. The resulting precipitate was suspended with 0.4mL of proteinase K and 32mg of DTT in 16mL of phosphate buffer, and shaken at 37 ℃ for 12 hours, and this operation was repeated 2 times. And after the enzymolysis reaction is completed, standing for 12 hours at 37 ℃, centrifuging, discarding the supernatant, washing for 3 times by using deionized water, and drying at 50 ℃ to obtain natural melanin particles with the particle size range of 200-400 nm.
Adding 100mg of attapulgite into 4mL of deionized water, ultrasonically dispersing for 20min, mechanically stirring for 2h, and repeating for 3 times. Adding 10mg of natural melanin granules into 4mL of deionized water, carrying out water bath at 50 ℃, carrying out ultrasonic dispersion for 10min, mechanically stirring for 2h, and repeating for 3 times. 4mL of attapulgite solution and 4mL of melanin granule solution are mixed in equal proportion, and stirred in a water bath at 50 ℃ for 6 h. Mixing chitosan and glacial acetic acid in a ratio of 1:1 in proportion, dissolving in deionized water to prepare a chitosan solution with the mass concentration of 1 wt%. Mixing 10mL of chitosan solution with the attapulgite-melanin solution (the mass ratio of the attapulgite-melanin composite component to the gel base material is 1.1:1), stirring for 24h, and pre-cooling in ice-water bath. In an ice-water bath, 0.5mL of ammonium sulfate (80mg/mL) and 0.5mL of tetramethylethylenediamine (20. mu.g/mL) were added in this order to the prepared mixed solution. The gel precursor is placed in a refrigerator at the temperature of-20 ℃ and reacted for 24 h. Taking out an attapulgite-melanin gel sample, washing with deionized water, and freeze-drying to obtain the final attapulgite-melanin hemostatic gel.
Example 2
20g of attapulgite (palygorskite) raw ore is taken and dissolved in 800mL of deionized water (mass ratio is 1: 40), ultrasonic stirring is carried out for 8h, and suction filtration is carried out. And (3) resuspending the attapulgite obtained by suction filtration in 200mL of deionized water, naturally settling for 10min, and taking supernatant to perform centrifugal separation at the rotating speed of 8000 rpm. And (4) drying the precipitate obtained by centrifugation in an oven at 80 ℃ for 8h to obtain the attapulgite.
Commercially available feathers or hairlines were washed with acetone 3 times, deionized water 3 times, and cut into pieces. The obtained feathers or hairs were added to 40mL of phosphate buffer (pH 6.8), 0.4g of Dithiothreitol (DTT) was added thereto, and the mixture was mixed well under N 2 Shaking at 37 deg.C for 12h under atmosphere. Add 1mL of 20mg/mL proteinase K and 0.2g DTT and continue shaking for 12 h. Centrifuging at 8000rpm, washing with deionized water for 3 times, and removing supernatant to obtain precipitate. The resulting pellet was mixed with 0.4mL of proteinase K and 32mg of DTT in 16mL of phosphate buffer, resuspended, and shaken for 12 hours. Centrifuging, removing supernatant, adding 6mL phosphate buffer and 0.12mL polyethylene glycol octyl phenyl ether, shaking for 6h, centrifuging, washing with methanol for 3 times, and washing with deionized water for 3 times. Obtained byThe precipitate was suspended with 0.4mL of proteinase K and 32mg of DTT in 16mL of phosphate buffer, and shaken at 37 ℃ for 12 hours, and this operation was repeated 2 times. And after the enzymolysis reaction is completed, standing for 12 hours at 37 ℃, centrifuging, discarding the supernatant, washing for 3 times by using deionized water, and drying at 50 ℃ to obtain natural melanin particles with the particle size range of 200-400 nm.
Adding 100mg of attapulgite into 4mL of deionized water, performing ultrasonic dispersion for 20 min-mechanically stirring for 2h, and repeating for 3 times. Adding 10mg of natural melanin granules into 4mL of deionized water, carrying out water bath at 50 ℃, carrying out ultrasonic dispersion for 10min, mechanically stirring for 2h, and repeating for 3 times. Mixing 4mL of attapulgite solution and 4mL of natural melanin granule solution at equal ratio, and stirring in water bath at 50 deg.C for 6 h. Mixing chitosan and glacial acetic acid in a ratio of 1:1 is dissolved in deionized water to prepare a chitosan solution with the mass concentration of 5 wt%. Mixing 10mL of chitosan solution with the attapulgite-melanin solution (the mass ratio of the attapulgite-melanin composite component to the gel base material is 0.22:1), stirring for 24h, and pre-cooling in ice water bath. In an ice-water bath, 1mL of ammonium sulfate (100mg/mL) and 1mL of tetramethylethylenediamine (20. mu.g/mL) were added in this order to the prepared mixed solution. The gel precursor was placed in a-20 ℃ freezer and allowed to react for 24 h. Taking out the attapulgite-melanin gel sample, thawing, washing with deionized water for 3 times, and naturally drying to obtain the final attapulgite-melanin hemostatic gel.
Example 3
20g of attapulgite (palygorskite) raw ore is taken and dissolved in 800mL of deionized water (mass ratio is 1: 40), ultrasonic stirring is carried out for 8h, and suction filtration is carried out. And (3) resuspending the attapulgite obtained by suction filtration in 200mL of deionized water, naturally settling for 10min, and taking supernatant to perform centrifugal separation at the rotating speed of 8000 rpm. And (4) drying the precipitate obtained by centrifugation in an oven at 80 ℃ for 8h to obtain the attapulgite.
Commercially available feather or hair is taken, washed with acetone for 3 times, washed with deionized water for 3 times, and cut into pieces. The obtained feathers or hairs were added to 40mL of phosphate buffer (pH 6.8), 0.4g of Dithiothreitol (DTT) was added thereto, and the mixture was mixed well under N 2 Shaking at 37 deg.C for 12h under atmosphere. 1mL of 20mg/mL protein was addedEnzyme K and 0.2g DTT, shaking was continued for 12 h. Centrifuging at 8000rpm, washing with deionized water for 3 times, and removing supernatant to obtain precipitate. The resulting pellet was mixed with 0.4mL proteinase K and 32mg DTT in 16mL phosphate buffer, resuspended, and shaken for 12 h. Centrifuging, removing supernatant, adding 6mL of phosphate buffer and 0.12mL of polyethylene glycol octyl phenyl ether, oscillating for 6h, centrifuging, washing with methanol for 3 times and deionized water for 3 times in sequence. The resulting precipitate was suspended with 0.4mL of proteinase K and 32mg of DTT in 16mL of phosphate buffer, and shaken at 37 ℃ for 12 hours, and this operation was repeated 2 times. And after the enzymolysis reaction is completed, standing for 12 hours at 37 ℃, centrifuging, discarding the supernatant, washing for 3 times by using deionized water, and drying at 50 ℃ to obtain natural melanin particles with the particle size range of 200-400 nm.
Adding 1000mg of attapulgite into 4mL of deionized water, ultrasonically dispersing for 20min, mechanically stirring for 2h, and repeating for 6 times. Adding 10mg of natural melanin granules into 4mL of deionized water, carrying out water bath at 50 ℃, carrying out ultrasonic dispersion for 10min, mechanically stirring for 2h, and repeating for 3 times. Mixing 4mL of attapulgite solution and 4mL of natural melanin granule solution at equal ratio, and stirring in water bath at 50 deg.C for 24 h. Mixing chitosan and glacial acetic acid in a ratio of 1:1 in deionized water to prepare a chitosan solution with the mass concentration of 10 wt%. Mixing 10mL of chitosan solution with the attapulgite-melanin solution (the mass ratio of the attapulgite-melanin composite component to the gel base material is 1.01:1), stirring for 24h, and pre-cooling in ice-water bath. In an ice-water bath, 1mL of ammonium sulfate (120mg/mL) and 1mL of tetramethylethylenediamine (20. mu.g/mL) were added in this order to the prepared mixed solution. The gel precursor was placed in a-10 ℃ refrigerator and reacted for 48 h. Taking out an attapulgite-melanin gel sample, washing with deionized water, and freeze-drying to obtain the final attapulgite-melanin hemostatic gel.
Comparative example 1
20g of attapulgite (palygorskite) raw ore is taken and dissolved in 800mL of deionized water (mass ratio is 1: 40), ultrasonic stirring is carried out for 8h, and suction filtration is carried out. And (3) resuspending the attapulgite obtained by suction filtration in 200mL of deionized water, naturally settling for 10min, and taking supernatant to perform centrifugal separation at the rotating speed of 8000 rpm. And (4) drying the precipitate obtained by centrifugation in an oven at 80 ℃ for 8 hours to obtain the attapulgite.
Comparative example 2
Commercially available feathers or hairlines were washed with acetone 3 times, deionized water 3 times, and cut into pieces. The obtained feathers or hairs were added to 40mL of phosphate buffer (pH 6.8), 0.4g of Dithiothreitol (DTT) was added thereto, and the mixture was mixed well under N 2 Shaking at 37 deg.C for 12h under atmosphere. Add 1mL of 20mg/mL proteinase K and 0.2g DTT and continue shaking for 12 h. Centrifuging at 8000rpm, washing with deionized water for 3 times, and removing supernatant to obtain precipitate. The resulting pellet was mixed with 0.4mL proteinase K and 32mg DTT in 16mL phosphate buffer, resuspended, and shaken for 12 h. Centrifuging, removing supernatant, adding 6mL phosphate buffer and 0.12mL polyethylene glycol octyl phenyl ether, shaking for 6h, centrifuging, washing with methanol for 3 times, and washing with deionized water for 3 times. The resulting precipitate was suspended with 0.4mL of proteinase K and 32mg of DTT in 16mL of phosphate buffer, and shaken at 37 ℃ for 12 hours, and this operation was repeated 2 times. And after the enzymolysis reaction is completed, standing for 12 hours at 37 ℃, centrifuging, discarding supernatant, washing for 3 times by using deionized water, and drying at 50 ℃ to obtain natural melanin particles with the particle size range of 200-400 nm.
Comparative example 3
20g of attapulgite (palygorskite) raw ore is taken and dissolved in 800mL of deionized water (mass ratio of 1: 40), ultrasonic stirring is carried out for 8h, and suction filtration is carried out. And (3) resuspending the attapulgite obtained by suction filtration in 200mL of deionized water, naturally settling for 10min, and taking supernatant to perform centrifugal separation at the rotating speed of 8000 rpm. And (4) drying the precipitate obtained by centrifugation in an oven at 80 ℃ for 8h to obtain the attapulgite.
Commercially available feathers or hairlines were washed with acetone 3 times, deionized water 3 times, and cut into pieces. The obtained feathers or hairs were added to 40mL of phosphate buffer (pH 6.8), 0.4g of Dithiothreitol (DTT) was added thereto, and the mixture was mixed well under N 2 Shaking at 37 deg.C for 12h under atmosphere. Add 1mL of 20mg/mL proteinase K and 0.2g DTT and continue shaking for 12 h. Centrifuging at 8000rpm, washing with deionized water for 3 times, and removing supernatant to obtain precipitate. The resulting precipitate was mixed with 0.4mL of proteinEnzyme K, 32mg DTT were added to 16mL of phosphate buffer, resuspended, and shaken for 12 h. Centrifuging, removing supernatant, adding 6mL phosphate buffer and 0.12mL polyethylene glycol octyl phenyl ether, shaking for 6h, centrifuging, washing with methanol for 3 times, and washing with deionized water for 3 times. The resulting precipitate was suspended with 0.4mL of proteinase K and 32mg of DTT in 16mL of phosphate buffer, and shaken at 37 ℃ for 12 hours, and this operation was repeated 2 times. And after the enzymolysis reaction is completed, standing for 12 hours at 37 ℃, centrifuging, discarding supernatant, washing for 3 times by using deionized water, and drying at 50 ℃ to obtain natural melanin particles with the particle size range of 200-400 nm.
4mL of 12mM Tris buffer was prepared, pH was adjusted to 8, and sonication was carried out for 1 h. Adding 10mg natural melanin granules into the solution, magnetically stirring for 2 h-ultrasonic dispersion for 10min at the temperature of 50 ℃ in a water bath, and repeating the operation for 3 times, wherein the ultrasonic power is 100w and the frequency is 40 kHz. 100mg of attapulgite nano-particles are dissolved in 4mL of deionized water and stirred for 12h at the temperature of 50 ℃ in a water bath. Mixing the natural melanin granule solution and attapulgite solution at equal ratio, performing ultrasonic dispersion for 10 min-magnetically stirring for 2h in water bath at 50 deg.C, repeating the operation for 6 times, with ultrasonic power of 100w and frequency of 40 kHz. Standing the obtained attapulgite-melanin composite solution for 24h, and drying in an oven at 50 deg.C to obtain attapulgite-melanin composite powder.
Comparative example 4
A control group of commercial ordinary gauze (Medical gauze) was prepared by taking commercially available ordinary gauze, sterilizing by ultraviolet irradiation for 1.5h, cutting into small round pieces with a diameter of 1.2cm, and operating in a sterile environment.
The hemostatic performance of the hemostatic material is evaluated by a mouse liver wound bleeding model, obvious wound bleeding is formed on the surface of the mouse liver by using an operating knife, and the hemostatic performance is evaluated by applying the same amount of the material prepared in example 2 and the materials prepared in comparative examples 1-4 to the surface of the wound. As shown in fig. 2, the attapulgite-melanin composite powder prepared in comparative example 3 has significant statistical advantages and exhibits shorter hemostatic time, compared to the attapulgite (Pal) in comparative example 1 and the natural Melanin Granules (MGs) in comparative example 2, which indicates that the attapulgite and the natural melanin composite have synergistic hemostatic performance; compared with the attapulgite-melanin composite powder (Kaolin-MGs) in the comparative example 3, the attapulgite-melanin hemostatic gel (Pal-MGs HGs) in the example 2 has the advantages that the hemostatic time can be further shortened, and the remarkable statistical advantages are achieved, so that the attapulgite-melanin hemostatic gel can realize better hemostatic performance based on the porous structure of the attapulgite-melanin hemostatic gel; and the attapulgite-melanin hemostatic gels (Pal-MGs HGs, example 2) also exhibited significant hemostatic performance advantages over the blank control (NC) and commercial plain gauze (Medical gauze, comparative example 4). In a word, the attapulgite-melanin hemostatic gel prepared by the invention has efficient and safe hemostatic performance based on the synergistic effect of the attapulgite, the natural melanin and the gel base material.
Claims (9)
1. An attapulgite-melanin hemostatic gel is characterized in that: is compounded by attapulgite-melanin composite components and gel base materials; the attapulgite-melanin composite component is prepared by compounding attapulgite and natural melanin particles.
2. Attapulgite-melanin haemostatic gel according to claim 1, characterized in that: the mass ratio of the attapulgite-melanin composite component to the gel base material is 0.2-1.2: 1.
3. attapulgite-melanin haemostatic gel according to claim 1, characterized in that: the mass ratio of the attapulgite to the natural melanin granules is 10-100: 1.
4. attapulgite-melanin haemostatic gel according to claim 1, characterized in that: the natural melanin granules are extracted from at least one of animal hair, feathers, fungi and bacteria.
5. Attapulgite-melanin haemostatic gel according to claim 1, characterized in that: the gel base material is selected from at least one of chitosan, gelatin and fibrin.
6. A method for preparing attapulgite-melanin hemostatic gel according to any one of claims 1 to 5, wherein: firstly, uniformly mixing an attapulgite solution and a natural melanin granule solution in a water bath; then adding a chitosan solution, stirring, carrying out ice-water bath precooling, and then adding ammonium sulfate and tetramethyl ethylene diamine to prepare a gel precursor; finally, the attapulgite-melanin hemostatic gel is obtained by freezing reaction, unfreezing, washing and drying.
7. The method of manufacturing according to claim 6, characterized in that: the temperature of the water bath is 45-55 ℃; the chitosan solution has a mass concentration of 1-10 wt%, the stirring time is 12-48h, and the temperature of the ice water bath is 0-4 ℃.
8. The method of claim 6, wherein: the temperature of the freezing reaction is-10 to-20 ℃, and the reaction time is 24 to 48 hours.
9. Use of the attapulgite-melanin hemostatic gel according to any one of claims 1 to 5 or the attapulgite-melanin hemostatic gel prepared by the preparation method according to any one of claims 6 to 8, characterized in that: the compound is used as a hemostatic active component to prepare an emergency wound hemostatic material, a hemostatic product, a wound repair material or a wound healing material.
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