CN114778515A - Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care product - Google Patents
Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care product Download PDFInfo
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- CN114778515A CN114778515A CN202210445723.7A CN202210445723A CN114778515A CN 114778515 A CN114778515 A CN 114778515A CN 202210445723 A CN202210445723 A CN 202210445723A CN 114778515 A CN114778515 A CN 114778515A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Abstract
The invention discloses a method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care products, belonging to the field of food detection. The invention determines the Raman characteristic peak of sibutramine and fenfluramine; then, pretreating the weight-reducing health-care product sample, and extracting by using an extraction solvent to obtain a solution to be detected; and then judging whether sibutramine and fenfluramine exist in the sample to be detected according to the Raman spectrogram of the solution to be detected. The method provided by the invention has the advantages that the lowest detection concentration of sibutramine and fenfluramine in the weight-losing health-care product is 25mg/kg, the qualitative analysis can be carried out on the sibutramine and fenfluramine in the weight-losing health-care product, and the detection time of a single sample is controlled within 2 min.
Description
Technical Field
The invention relates to a method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care products, belonging to the field of food detection.
Background
In recent years, with the continuous improvement of living standard, the obesity problem becomes more serious, and troubles many people. Based on the mind, a plurality of merchants have promoted weight-reducing health care products which are favored by consumers, however, some merchants often incorporate substances which are prohibited from being detected by national regulations into the weight-reducing health care products under the drive of benefits to obtain a larger benefit space. At present, substances mainly added in weight-losing health care products by illegal merchants are sibutramine and fenfluramine, wherein the sibutramine is a medicine acting on 5-hydroxytryptamine and noradrenaline pathways and can enhance satiety of a human body; fenfluramine is also a drug acting on 5-hydroxytryptamine, and can reduce appetite and further achieve the purpose of losing weight. The two medicines have strong side effects, and when a consumer takes the weight-reducing health-care product containing the medicines, symptoms such as diarrhea, headache, hypotension, epilepsy and the like can appear, so that the addition of the two substances in the weight-reducing health-care product is forbidden in China. At present, detection methods for forbidden additives in health care products mainly comprise TLC (thin layer chromatography) methods, chromatography methods and the like, and the methods have the problems of complex operation, long detection time and the like, and are not suitable for large-scale field screening, so that a method for rapidly and accurately screening the forbidden additives is needed to be established.
Surface Enhanced Raman Scattering (SERS) is a technology combining nanotechnology with ordinary Raman spectroscopy, and a nanostructure is used to enhance Raman Scattering of an analyte and quench background fluorescence, so that the SERS has the advantages of high sensitivity, high screening speed and the like, and is widely used for detecting pollutants in food in recent years, however, the SERS detection method is generally limited by a complex food substrate and an Enhanced substrate, and further application of the SERS detection method in the field of food detection is limited. The traditional SERS substrate is mainly a noble metal sol, the substrate is expensive and poor in stability, and further application of the SERS technology is limited to a certain extent, so that the development of a novel substrate is urgently needed to expand the application range.
Au @ ZIF-8 is a metal-organic framework (MOFs) material, is a novel nano porous functional material, is commonly used in the field of catalysis, and has not been applied to the surface enhanced Raman technology.
Disclosure of Invention
[ problem ] to
At present, detection methods for forbidden additives in health care products mainly comprise TLC (thin layer chromatography) methods, chromatography methods and the like, and the methods have the problems of complex operation, long detection time and the like, are not suitable for large-scale field screening, so that a method for rapidly and accurately screening the forbidden additives is needed to be established. The SERS detection is rapid and convenient, the sensitivity is high, and the detection time is short, however, the traditional SERS method uses noble metal sol as a reinforced substrate, so the detection limit of the method is relatively higher than that of a high performance liquid chromatography tandem mass spectrometry, and how to develop a novel substrate and reduce the detection limit is a problem worth discussing.
[ solution ]
In order to solve at least one problem, the invention provides a method for simultaneously detecting sibutramine and fenfluramine by using a surface enhanced raman method, which is simple to operate, has short detection time and can effectively improve the detection efficiency under the condition of ensuring the accuracy.
The first purpose of the invention is to provide a method for qualitatively detecting sibutramine and fenfluramine, which comprises the following steps:
(1) preparing a surface-enhanced Raman substrate Au @ ZIF-8;
(2) mixing the sample liquid with Au @ ZIF-8, then carrying out surface enhanced Raman spectrum detection, and acquiring corresponding spectral information;
(3) if the spectral information contains the following characteristic peaks: 631. 731, 821, 1091, 1137 and 1594cm-1If the sample solution contains sibutramine; if the spectral information contains the following characteristic peaks: 753. 1361, 1446 and 1601cm-1If the sample solution contains fenfluramine; if the spectral information contains the following characteristic peaks: 731. 1137 and 1361cm-1And the sample solution contains sibutramine and fenfluramine at the same time.
In one embodiment of the present invention, the presence or absence of sibutramine and fenfluramine is determined based on the presence of the qualitative peaks;
in one embodiment of the invention, in the step (1), the preparation method of the surface enhanced Raman substrate Au @ ZIF-8 comprises the steps of wrapping the surface of gold nanoparticles with polyvinylpyrrolidone as a bridging agent, and sequentially adding a zinc nitrate solution and a 2-methylimidazole solution into the gold nanoparticles modified by the bridging agent to finally obtain Au @ ZIF-8.
In one embodiment of the invention, the preparation method of the surface enhanced Raman substrate Au @ ZIF-8 comprises the following steps:
A. preparing gold sol: mixing the potassium chloroaurate solution with ultrapure water, heating, stirring and heating to boil, then adding the trisodium citrate solution, and continuously stirring to obtain a dark red gold sol solution;
B. modification of gold sol: mixing the prepared gold sol solution with 2.5% of polyvinylpyrrolidone aqueous solution, and stirring to obtain polyvinylpyrrolidone modified gold sol;
C. obtaining a novel core-shell substrate: taking the modified gold sol, centrifuging, removing supernatant after centrifuging, adding methanol, and uniformly mixing to obtain a modified gold sol solution; and adding the modified gold sol solution into a zinc nitrate solution, stirring, continuously adding a 2-methylimidazole solution, stirring, standing, centrifuging after the reaction is finished, washing a product with methanol, and drying the product to obtain the novel core-shell substrate Au @ ZIF-8.
In one embodiment of the invention, the novel core-shell substrate Au @ ZIF-8 is used when Au @ ZIF-8 is dispersed in methanol.
In one embodiment of the present invention, in step a, the gold sol and the 2.5% polyvinylpyrrolidone aqueous solution are used in a volume ratio of 1: 4.
In one embodiment of the invention, in the step A, the concentration of the zinc nitrate solution and the concentration of the 2-methylimidazole solution are both 20-30 mmol/L; 25mmol/L are preferred.
In one embodiment of the invention, in the step a, the volume ratio of the zinc nitrate solution to the 2-methylimidazole solution is 1: 3.
In one embodiment of the present invention, in step A, the amount of the zinc nitrate solution is 0.1mL, and the amount of the 2-methylimidazole solution is 0.3 mL.
In one embodiment of the present invention, in step a, the amount of the zinc nitrate solution is 0.8mL, and the amount of the 2-methylimidazole solution is 2.4 mL.
In one embodiment of the present invention, in step a, the amount of the zinc nitrate solution is 4mL, and the amount of the 2-methylimidazole solution is 12 mL.
In one embodiment of the invention, in the step a, the volume ratio of the zinc nitrate solution to the modified gold sol is 0.1-4: 20, and the preferred volume ratio is 0.1: 20.
In one embodiment of the invention, the preparation method of the Au @ ZIF-8 in the step A specifically comprises the following steps: mixing 10mL of prepared gold sol with 40 mL2.5% of polyvinylpyrrolidone aqueous solution, and stirring for 24h to obtain polyvinylpyrrolidone modified gold sol; taking 15mL of modified gold sol, centrifuging (10000r, 10min), centrifuging, removing supernatant, adding 5mL of methanol, and mixing uniformly to obtain the modified gold sol solution. And (2) respectively adding 0.1mL of 25mmol/L zinc nitrate solution into the modified gold sol solution, stirring for 5min, continuously adding 0.3mL of 25 mmol/L2-methylimidazole solution, stirring for 5min, standing for 15min, centrifuging (10000r, 10min) after the reaction is finished, washing for 3 times by using methanol, and putting the product into a 60 ℃ oven for overnight drying to obtain the Au @ ZIF-8 material.
In an embodiment of the present invention, the preparation method of the gold sol in step a specifically comprises: mixing 3mL of 10mg/mL potassium chloroaurate solution with 47mL of ultrapure water, heating, stirring and heating to boil, adding 2mL of 1% concentrated trisodium citrate solution, and continuously stirring for 20min to obtain a deep red gold sol solution.
In one embodiment of the present invention, the sample liquid in step (2) is obtained by mixing a sample to be tested and an extraction solvent uniformly, and then performing ultrasonic treatment and centrifugation; and obtaining a sample solution.
In one embodiment of the present invention, the sample to be tested is a weight-loss health product.
In an embodiment of the present invention, the sample to be tested is a pretreated weight-reducing health product, specifically, the weight-reducing health product is ground to obtain the ground weight-reducing health product.
In one embodiment of the invention, the extraction solvent is methanol.
In one embodiment of the present invention, the ratio of the amount of the extraction solvent to the sample to be tested in mL/g is 10: 1.
in one embodiment of the present invention, the preparation process of the sample liquid is: accurately weighing 1.000g of weight-losing health-care products, grinding the weight-losing health-care products into powder, putting the powder into a centrifuge tube, adding 10mL of methanol, fully oscillating, performing ultrasonic treatment for 10min, and centrifuging at 10000 r/min.
In one embodiment, the centrifugation is 10000r/min for 10 min.
In one embodiment of the invention, the mixing is by vortexing.
In one embodiment of the present invention, the raman spectrum detection conditions in step (2) are: scanning with a laser Raman spectrometer with 785nm excitation light source for 3s for 2 times.
In one embodiment of the invention, the Au @ ZIF-8 in the step (2) is prepared by mixing a solution and a sample solution; the solvent is methanol.
In one embodiment of the present invention, the conditions under which the Au @ ZIF-8 is prepared as a solution in step (2) are: 5mL of a solvent was used for dispersion per 30mg of gold nanoparticles prepared from potassium chloroaurate.
In one embodiment of the invention, the volume ratio of the sample solution to the Au @ ZIF-8 in the step (2) is 1: 1-4, further optimized to 1: 3.
in one embodiment of the invention, in the step (2), the sample solution and the Au @ ZIF-8 are uniformly mixed and then subjected to Raman detection within 2 min.
The invention also provides application of the method in the field of food detection.
[ advantageous effects ]
(1) The method can simultaneously detect sibutramine and fenfluramine in the weight-losing health-care products.
(2) The lowest detection concentration of sibutramine and fenfluramine in the weight-losing health-care products detected by the method is 25 mg/kg.
(3) The method can be used for qualitatively analyzing the forbidden addition of sibutramine and fenfluramine in the weight-reducing health care products, the detection time of a single sample is controlled within 2min, and a quick and convenient detection method is provided for quickly and qualitatively screening the forbidden addition of sibutramine and fenfluramine in the weight-reducing health care products.
Drawings
FIG. 1 shows a comparison of Sibutramine enhanced Raman spectra with enhanced Raman spectra; wherein a is Sibutramine solid Raman spectrum and b is enhanced Raman spectrum.
FIG. 2 is a comparison of fenfluramine enhanced Raman spectra and enhanced Raman spectra; wherein a is a fenfluramine solid Raman spectrum, and b is an enhanced Raman spectrum.
Fig. 3 is raman spectra of mixed solutions of sibutramine and fenfluramine at different concentrations; wherein from a to g are respectively a substrate, 20mg/L, 25mg/L, 50mg/L, 100mg/L, 250mg/L and 500mg/L solution.
FIG. 4 is a comparison graph of Raman spectra of the weight-reducing health product with different labeling levels and the labeled extract; wherein a is a blank weight-reducing health care product extract, b is 20mg/kg, c is 25mg/kg, d is 50mg/kg, e is 100mg/kg, f is 250mg/kg, and g is 500 mg/kg.
FIG. 5 is a comparison graph of Raman intensity of a liquid to be measured and Au @ ZIF-8 mixed at different ratios, wherein a is 1:1, b is 1:2, c is 1:3, and d is 1: 4.
FIG. 6 is a comparison of Raman intensity at different mixing times, where a is 1min, b is 2min, c is 3min, and d is 4 min.
Detailed Description
The following description is of preferred embodiments of the invention, and it is to be understood that the embodiments are for the purpose of illustrating the invention better and are not to be taken in a limiting sense.
Example 1
A method for qualitatively detecting sibutramine and fenfluramine in weight-losing health-care products comprises the following steps:
(1) preparing a Raman enhancement substrate:
soaking a used glassware (a round-bottom flask) and a used rotor in aqua regia for 12 hours, taking out, washing with ultrapure water for three times, and drying in a 60 ℃ oven for later use; preparing trisodium citrate aqueous solution with the mass fraction of 1% and potassium chloroaurate solution with the mass fraction of 10mg/mL for later use; heating the oil bath pan to 120 ℃ and keeping the temperature constant; setting parameters of a magnetic stirrer: 565 r/min;
adding 47mL of ultrapure water and 3mL of potassium chloroaurate into a round-bottom flask, and fully mixing; putting the round-bottom flask into a 120 ℃ oil bath pot, stirring by using a magnetic stirrer and keeping the temperature constant until the solution is boiled; adding 2mL of 1% trisodium citrate aqueous solution, and continuously stirring at constant temperature of 120 ℃ for 20 min; cooling to normal temperature to obtain gold sol for later use;
and secondly, mixing 10mL of prepared gold sol with 40mL of 2.5% polyvinylpyrrolidone aqueous solution, and stirring for 24 hours to obtain polyvinylpyrrolidone modified gold sol.
And thirdly, taking 15mL of the modified gold sol, centrifuging (10000r, 10min), centrifuging, removing supernatant, adding 5mL of methanol, and uniformly mixing to obtain the polyvinylpyrrolidone modified gold sol. Taking polyvinylpyrrolidone modified gold sol, adding 0.1mL of 25mmol/L zinc nitrate solution, stirring for 5min, continuously adding 0.3mL of 25 mmol/L2-methylimidazole solution, stirring for 5min, standing for 15min, centrifuging (10000r, 10min) after the reaction is finished, washing for 3 times by using methanol, and putting the product into a 60 ℃ oven for overnight drying to obtain Au @ ZIF-8 materials with different thicknesses. When in use, Au @ ZIF-8 is dispersed in 5mL of methanol.
(2) Qualitative spectrum peaks of standard sibutramine and fenfluramine:
weighing 5mg of sibutramine standard substance, dissolving the sibutramine standard substance by using methanol, transferring the solution to a 5mL brown volumetric flask to obtain a 1mg/mL standard solution, and storing the solution at 4 ℃ for later use;
weighing 5mg of fenfluramine standard substance, dissolving the fenfluramine standard substance by using methanol, transferring the solution to a 5mL brown volumetric flask to obtain a 1mg/mL standard solution, and storing the solution at 4 ℃ for later use;
weighing 5mg of sibutramine and fenfluramine standard substances, dissolving the sibutramine and fenfluramine standard substances by using methanol, transferring the solution to a 5mL brown volumetric flask to obtain a 1mg/mL mixed solution, and diluting the mixed solution step by step into a mixed solution with the concentration of 500, 250, 100, 50, 25 and 20mg/L to dilute the mixed solution to 4 ℃ for storage and later use;
mixing 10 mu L of sibutramine solution with 30 mu L of Au @ ZIF-8, dripping the mixture onto a glass sheet wrapped by tinfoil, and scanning by using a laser Raman spectrometer with 785nm of excitation light source for 3s and 2 times to obtain a Raman spectrum of the sibutramine standard solution (figure 1);
as can be seen from fig. 1: characteristic peaks 631, 731, 821, 1091, 1137 and 1594cm of sibutramine-1。
In the range of 400-2000 cm-1In the range of 631cm-1And 821cm-1The position of the vibration is 731cm-1Ascribed to-CH in cyclobutane2Swing in plane of (3), 1091cm-1Vibration of benzene ring at 1137cm-1The attribution is C-H vibration in the benzene ring surface, 1594cm-1The double bond stretching vibration of the internal olefin is adopted.
Mixing 10 mu L of fenfluramine solution with 30 mu L of Au @ ZIF-8, then dropwise adding the mixture onto a glass sheet wrapped by tinfoil, and scanning by using a laser Raman spectrometer with 785nm as an excitation light source for 3s, wherein the scanning times are 2 times, so as to obtain a Raman spectrum of the fenfluramine standard solution (figure 2);
as can be seen from fig. 2: characteristic peaks 753, 1361, 1446 and 1601cm of fenfluramine-1。
In the range of 400-2000 cm-1, wherein 753cm-1Vibration of benzene ring at 1341cm-1At 1446cm under C-F telescopic vibration-1And 1601cm-1C ═ C where benzene ring is located vibrates telescopically.
Mixing 10 mu L of mixed solution with 30 mu L of Au @ ZIF-8, then dropwise adding the mixture onto a glass sheet wrapped by tinfoil, and scanning by using a laser Raman spectrometer with 785nm as an excitation light source for 3s, wherein the scanning times are 2 times, so as to obtain Raman spectra of the mixed solution with different concentrations (figure 3);
in the range of 400-2000 cm < -1 >, the characteristic peaks of the two are 731, 1137 and 1361cm when the two exist at the same time-1。
(3) Actual sample detection
Accurately weighing 1.000g of weight-losing health-care products, grinding the weight-losing health-care products into powder, putting the powder into a centrifuge tube, adding 10mL of methanol, fully oscillating, performing ultrasonic treatment for 10min, centrifuging at 10000rpm, and taking supernate for Raman detection;
mixing 10 μ L of labeling extractive solution with 30 μ L of Au @ ZIF-8, dripping onto tinfoil wrapped glass sheet, scanning with 785nm laser Raman spectrometer with 3s excitation light source for 2 times to obtain Raman spectrogram, as shown in FIG. 4, at labeling concentration of 25mg/kg, characteristic peaks of sibutramine and fenfluramine can be observed, 731, 1137cm-1The characteristic peak can judge the existence of sibutramine, 1361cm-1The characteristic peak can judge that fenfluramine exists, so the detection limit is 25 mg/kg.
Example 2
The volume ratio of the mixed solution and Au @ ZIF-8 in example 1 was adjusted to 1:1, 1:2, 1:3, and 1:4, and the other volume ratios were kept the same as those in example 1, and the detection was performed.
The detection result is as follows: mixing the gold sol and the solution to be detected according to different proportions, wherein the result is shown in fig. 5, and the volume of the gold sol and the volume of the solution to be detected are 1: at 2, the signal intensity is highest.
Example 3
The mixing time of the mixed solution and Au @ ZIF-8 in the example 1 was adjusted to 1min, 2min, 3min and 4min, and the others were kept the same as those in the example 1, and then the detection was performed.
The detection result is as follows: and mixing Au @ ZIF-8 with the solution to be detected according to different time, wherein the result is shown in figure 6, the mixing time is 2min, and the signal intensity is highest.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A method for qualitatively detecting sibutramine and fenfluramine is characterized by comprising the following steps:
(1) preparing a surface-enhanced Raman substrate Au @ ZIF-8;
(2) mixing the sample liquid with Au @ ZIF-8, then carrying out surface enhanced Raman spectrum detection, and acquiring corresponding spectral information;
(3) if the spectral information contains the following characteristic peaks: 631. 731, 821, 1091, 1137 and 1594cm-1If so, the sample solution contains sibutramine; if the spectral information contains the following characteristic peaks: 753. 1361, 1446 and 1601cm-1If so, the sample solution contains fenfluramine; if the spectral information contains the following characteristic peaks: 731. 1137 and 1361cm-1And then the sample solution contains sibutramine and fenfluramine at the same time.
2. The method as claimed in claim 1, wherein in the step (1), the surface enhanced raman substrate Au @ ZIF-8 is prepared by wrapping the surface of gold nanoparticles with polyvinylpyrrolidone as a bridging agent, and adding a zinc nitrate solution and a 2-methylimidazole solution into the bridging agent modified gold nanoparticles in sequence to obtain Au @ ZIF-8.
3. The method according to claim 1, wherein the sample liquid in step (2) is obtained by mixing a sample to be tested with an extraction solvent uniformly, performing ultrasonic treatment, centrifuging, and taking a supernatant; obtaining a sample solution.
4. The method of claim 3, wherein the extraction solvent is methanol.
5. The method according to claim 3, characterized in that the ratio of the amount of extraction solvent to the sample to be tested in mL/g is 10: 1.
6. the method according to claim 1, wherein the Raman spectroscopy detection conditions in step (2) are: scanning with 785nm laser Raman spectrometer for 3s for 2 times.
7. The method of claim 1, wherein the Au @ ZIF-8 in step (2) is formulated as an Au @ ZIF-8 solution and then mixed with the sample fluid; the solvent is methanol.
8. The method of claim 7, wherein the volume ratio of the sample solution to the Au @ ZIF-8 solution in step (2) is 1: 1-4.
9. The method according to any one of claims 1 to 8, wherein the Raman detection is performed within 2min after the sample solution and the Au @ ZIF-8 are uniformly mixed in step (2).
10. Use of the method of any one of claims 1 to 9 in the field of food testing.
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| CN202210445723.7A CN114778515A (en) | 2022-04-26 | 2022-04-26 | Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care product |
| PCT/CN2022/141667 WO2023207168A1 (en) | 2022-04-26 | 2022-12-23 | Method for simultaneously detecting sibutramine and fenfluramine in weight-loss health-care products |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023207168A1 (en) * | 2022-04-26 | 2023-11-02 | 江南大学 | Method for simultaneously detecting sibutramine and fenfluramine in weight-loss health-care products |
| CN117554532A (en) * | 2024-01-10 | 2024-02-13 | 未名环境分子诊断（常熟）有限公司 | Sibutramine detection method |
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| CN104458693B (en) * | 2013-09-25 | 2018-06-01 | 同方威视技术股份有限公司 | For the method for measuring Raman spectrum of illicit drugs inspection |
| CN106198482B (en) * | 2015-05-04 | 2019-07-05 | 清华大学 | The method for whether being added with Western medicine in detection health care product based on Raman spectrum |
| CN107436300B (en) * | 2016-05-26 | 2019-08-16 | 中国科学院化学研究所 | Surface enhanced Raman scattering substrate material and preparation method thereof |
| CN111380855A (en) * | 2018-12-29 | 2020-07-07 | 同方威视技术股份有限公司 | Method for screening illegal drugs in health care products on site |
| CN109967758A (en) * | 2019-03-18 | 2019-07-05 | 北京信息科技大学 | The preparation method of ZIF-8/Au composite surface enhancing Raman substrate |
| CN112730375A (en) * | 2020-12-16 | 2021-04-30 | 厦门大学 | Method for detecting VOC gas by using MOF-coated gold nanoparticles through enhanced Raman spectroscopy |
| CN113857486A (en) * | 2021-08-17 | 2021-12-31 | 中国科学院化学研究所 | Surface-enhanced Raman scattering substrate material and preparation method and application thereof |
| CN114994009A (en) * | 2022-04-20 | 2022-09-02 | 天津科技大学 | Preparation method and application of surface enhanced Raman substrate based on Ag @ ZIF-8 core-shell nanochain |
| CN114778515A (en) * | 2022-04-26 | 2022-07-22 | 江南大学 | Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care product |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2023207168A1 (en) * | 2022-04-26 | 2023-11-02 | 江南大学 | Method for simultaneously detecting sibutramine and fenfluramine in weight-loss health-care products |
| CN117554532A (en) * | 2024-01-10 | 2024-02-13 | 未名环境分子诊断（常熟）有限公司 | Sibutramine detection method |
| CN117554532B (en) * | 2024-01-10 | 2024-04-16 | 未名环境分子诊断（常熟）有限公司 | Sibutramine detection method |
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